MD02G1261300.v1.1

Dual specificity phosphatase, catalytic domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0013708
Physical Location & Seq
Reverse (-)
31515784 .. 31519552
3769 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1261300.v1.1.491

Sequence Viewer

Length: 726 bp
ATGGGTGTGGGTCTATCAGTTCTGATAGGGTTGAAAGCAGCAGTCTTGTTCTCGTTGTTTGCATTCCTCAGAAGCTCTGGTTTCACTGTGCTTTCCATACCATTTTTGTACGCCGCTCTGGTATCCGCGTTGGTTTCACTTGCTGCTCACCCATCCATAGACCTTCCCATGCTGCTGGGGAAGAACCCAGATGGGAGTTTCCCCGTTTGGTCGATTATAATGTTCAGCCCCTATTTGTATTTCGTTCGCTTTTTCTCGGCGCTGCGGAGATTGCGGAGTAGGGAGGCTCCGTATACTGAAATCTGTGAGGGTTTGTTCGTCGGCGGGTGGCCTTCTTCGCCGGATAAACTTCCACCAGGGGATCCTGCTATTGTTGATTGCACTTGTGAATTGCCAAGAAAGCCTGAGTTTTCAGGGCATTCTTATTTGTGTGTGCCAACATGGGACACAAGGGCTCCTCCACCGGCTGCCATTGAATCCGCTGTCAAGTGGGCTTGCCGGAAAAGGGATCAGAATAGGCCTGTTTTCGTCCACTGCGCGTACGGTCATGGGAGAAGTGTAGCTGTCATGTGTGCACTACTAGTTGCTCTTGGGGTGGCAGAGGACTGGAAAAATGCTGAGAAACTAATTCGAGAGAAACGTCCTTACATTAGGATGAATGCTCTACACCGCATGGCTCTGGAAGAGTGGTCAAAACATCGTTTATCTCCTTATAAGAAGAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DSPc

Protein Analysis

242

Amino Acids

26.88

Weight (kDa)

9.39

Isoelectric Point (pI)

48.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DSPc PF00782 124 - 232 1.4e-09 Dual specificity phosphatase, catalytic domain
Tc-R-P PF22785 138 - 198 4.5e-07 Polymorphic toxin system, DSP-PTPase phosphatase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013708)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g05130
malus_domestica MD02G1261300.v1.1 MD04G1248500.v1.1 MD07G1059500.v1.1
prunus_persica Prupe.2G067300_v2.0.a1
pyrus_communis pycom02g22340 pycom07g04560 pycom07g04570
rosa_chinensis RchiOBHm_Chr1g0331721
rosa_laevigata RLG00000029719
rosa_multiflora Rmu_co8516993.1_g000001
rosa_roxburghii Rroxscaffold_4G00318850
rosa_rugosa Rorug01G0095900
rosa_samantha Rh1AG119600 Rh1BG091200 Rh1CG114700 Rh1DG125100
rosa_wichuraiana Rw1G009670

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 218, 714
AccBSI CCGCTC 1 cut(s) 116
AccI GTMKAC 1 cut(s) 293
AccII CGCG 2 cut(s) 128, 539
AciI CCGC 7 cut(s) 114, 126, 265, 274, 324, 480, 670
AclWI GGATC 3 cut(s) 356, 369, 516
AfaI GTAC 2 cut(s) 110, 542
AfiI CCNNNNNNNGG 1 cut(s) 505
AgsI TTSAA 2 cut(s) 34, 476
AhlI ACTAGT 1 cut(s) 580
AjnI CCWGG 1 cut(s) 355
AluBI AGCT 2 cut(s) 75, 563
AluI AGCT 2 cut(s) 75, 563
Alw21I GWGCWC 1 cut(s) 577
Alw44I GTGCAC 1 cut(s) 573
AlwI GGATC 3 cut(s) 356, 369, 516
AoxI GGCC 2 cut(s) 329, 518
ApaLI GTGCAC 1 cut(s) 573
ApeKI GCWGC 5 cut(s) 38, 143, 172, 262, 467
AspLEI GCGC 2 cut(s) 262, 539
AsuHPI GGTGA 1 cut(s) 140
BaeGI GKGCMC 1 cut(s) 577
BamHI GGATCC 1 cut(s) 361
BanII GRGCYC 1 cut(s) 457
Bbv12I GWGCWC 1 cut(s) 577
BbvI GCAGC 5 cut(s) 50, 130, 159, 249, 454
BccI CCATC 2 cut(s) 160, 185
BciT130I CCWGG 1 cut(s) 357
BciVI GTATCC 1 cut(s) 133
BcuI ACTAGT 1 cut(s) 580
BfaI CTAG 1 cut(s) 581
BfoI RGCGCY 1 cut(s) 263
BfuI GTATCC 1 cut(s) 133
BisI GCNGC 6 cut(s) 39, 114, 144, 173, 263, 468
BlsI GCNGC 6 cut(s) 40, 115, 145, 174, 264, 469
Bme1390I CCNGG 1 cut(s) 357
BmiI GGNNCC 3 cut(s) 288, 363, 456
BmrFI CCNGG 1 cut(s) 357
BsaJI CCNNGG 1 cut(s) 356
BsaXI ACNNNNNCTCC 4 cut(s) 275, 305, 439, 469
Bsc4I CCNNNNNNNGG 1 cut(s) 505
Bse118I RCCGGY 1 cut(s) 463
Bse1I ACTGG 1 cut(s) 611
BseBI CCWGG 1 cut(s) 357
BseDI CCNNGG 1 cut(s) 356
BseGI GGATG 2 cut(s) 152, 660
BseLI CCNNNNNNNGG 1 cut(s) 505
BseMII CTCAG 3 cut(s) 82, 396, 609
BseNI ACTGG 1 cut(s) 611
BseRI GAGGAG 1 cut(s) 447
BseSI GKGCMC 1 cut(s) 577
BseXI GCAGC 5 cut(s) 50, 130, 159, 249, 454
BseYI CCCAGC 1 cut(s) 175
Bsh1236I CGCG 2 cut(s) 128, 539
BshFI GGCC 2 cut(s) 331, 520
BsiHKAI GWGCWC 1 cut(s) 577
BsiSI CCGG 3 cut(s) 341, 464, 499
BsiWI CGTACG 1 cut(s) 540
BslFI GGGAC 1 cut(s) 458
BslI CCNNNNNNNGG 1 cut(s) 505
BsmFI GGGAC 1 cut(s) 458
BsmI GAATGC 3 cut(s) 62, 418, 664
BsnI GGCC 2 cut(s) 331, 520
Bsp1286I GDGCHC 2 cut(s) 457, 577
Bsp143I GATC 2 cut(s) 361, 508
BspACI CCGC 7 cut(s) 114, 126, 265, 274, 324, 480, 670
BspANI GGCC 2 cut(s) 331, 520
BspCNI CTCAG 3 cut(s) 81, 397, 610
BspFNI CGCG 2 cut(s) 128, 539
BspLI GGNNCC 3 cut(s) 288, 363, 456
BspPI GGATC 3 cut(s) 356, 369, 516
BsrBI CCGCTC 1 cut(s) 116
BsrFI RCCGGY 1 cut(s) 463
BsrI ACTGG 1 cut(s) 611
BssAI RCCGGY 1 cut(s) 463
BssECI CCNNGG 1 cut(s) 356
BssMI GATC 2 cut(s) 361, 508
BssNAI GTATAC 1 cut(s) 294
Bst1107I GTATAC 1 cut(s) 294
Bst2UI CCWGG 1 cut(s) 357
Bst4CI ACNGT 2 cut(s) 88, 545
Bst6I CTCTTC 1 cut(s) 678
BstC8I GCNNGC 1 cut(s) 496
BstDEI CTNAG 3 cut(s) 68, 405, 618
BstF5I GGATG 2 cut(s) 152, 660
BstFNI CGCG 2 cut(s) 128, 539
BstH2I RGCGCY 1 cut(s) 263
BstHHI GCGC 2 cut(s) 262, 539
BstKTI GATC 2 cut(s) 364, 511
BstMBI GATC 2 cut(s) 361, 508
BstMWI GCNNNNNNNGC 3 cut(s) 271, 337, 400
BstNI CCWGG 1 cut(s) 357
BstSCI CCNGG 1 cut(s) 355
BstSLI GKGCMC 1 cut(s) 577
BstUI CGCG 2 cut(s) 128, 539
BstV1I GCAGC 5 cut(s) 50, 130, 159, 249, 454
BstX2I RGATCY 1 cut(s) 361
BstXI CCANNNNNNTGG 1 cut(s) 175
BstYI RGATCY 1 cut(s) 361
BstZ17I GTATAC 1 cut(s) 294
BsuI GTATCC 1 cut(s) 133
BsuRI GGCC 2 cut(s) 331, 520
BtsCI GGATG 2 cut(s) 152, 660
BtsI GCAGTG 1 cut(s) 532
BtsIMutI CAGTG 2 cut(s) 84, 532
Cac8I GCNNGC 1 cut(s) 496
CfoI GCGC 2 cut(s) 262, 539
Cfr10I RCCGGY 1 cut(s) 463
Csp6I GTAC 2 cut(s) 109, 541
CviAII CATG 5 cut(s) 169, 441, 548, 568, 673
CviQI GTAC 2 cut(s) 109, 541
DdeI CTNAG 3 cut(s) 68, 405, 618
DpnI GATC 2 cut(s) 363, 510
DpnII GATC 2 cut(s) 361, 508
Eam1104I CTCTTC 1 cut(s) 678
EarI CTCTTC 1 cut(s) 678
Eco147I AGGCCT 1 cut(s) 520
Eco24I GRGCYC 1 cut(s) 457
EcoRII CCWGG 1 cut(s) 355
EcoT38I GRGCYC 1 cut(s) 457
FaeI CATG 5 cut(s) 172, 444, 551, 571, 676
FaqI GGGAC 1 cut(s) 458
FatI CATG 5 cut(s) 168, 440, 547, 567, 672
FauI CCCGC 1 cut(s) 317
FblI GTMKAC 1 cut(s) 293
Fnu4HI GCNGC 6 cut(s) 39, 114, 144, 173, 263, 468
FokI GGATG 2 cut(s) 139, 667
FriOI GRGCYC 1 cut(s) 457
Fsp4HI GCNGC 6 cut(s) 39, 114, 144, 173, 263, 468
FspBI CTAG 1 cut(s) 581
GlaI GCGC 2 cut(s) 261, 538
GluI GCNGC 6 cut(s) 39, 114, 144, 173, 263, 468
GsaI CCCAGC 1 cut(s) 179
HaeII RGCGCY 1 cut(s) 263
HaeIII GGCC 2 cut(s) 331, 520
HapII CCGG 3 cut(s) 341, 464, 499
HhaI GCGC 2 cut(s) 262, 539
Hin1II CATG 5 cut(s) 172, 444, 551, 571, 676
Hin6I GCGC 2 cut(s) 260, 537
HinP1I GCGC 2 cut(s) 260, 537
HinfI GANTC 1 cut(s) 476
HpaII CCGG 3 cut(s) 341, 464, 499
HphI GGTGA 1 cut(s) 140
Hpy166II GTNNAC 3 cut(s) 294, 532, 575
Hpy188I TCNGA 3 cut(s) 24, 71, 513
Hpy188III TCNNGA 2 cut(s) 632, 680
Hpy8I GTNNAC 3 cut(s) 294, 532, 575
Hpy99I CGWCG 1 cut(s) 323
HpyAV CCTTC 2 cut(s) 173, 342
HpyCH4III ACNGT 2 cut(s) 88, 545
HpyCH4IV ACGT 1 cut(s) 640
HpyCH4V TGCA 3 cut(s) 62, 381, 575
HpyF10VI GCNNNNNNNGC 3 cut(s) 271, 337, 400
HpyF3I CTNAG 3 cut(s) 68, 405, 618
HpySE526I ACGT 1 cut(s) 640
Hsp92II CATG 5 cut(s) 172, 444, 551, 571, 676
HspAI GCGC 2 cut(s) 260, 537
Kzo9I GATC 2 cut(s) 361, 508
LmnI GCTCC 2 cut(s) 292, 460
Lsp1109I GCAGC 5 cut(s) 50, 130, 159, 249, 454
MaeI CTAG 1 cut(s) 581
MaeII ACGT 1 cut(s) 640
MalI GATC 2 cut(s) 363, 510
MbiI CCGCTC 1 cut(s) 116
MboI GATC 2 cut(s) 361, 508
MboII GAAGA 3 cut(s) 193, 327, 695
MflI RGATCY 1 cut(s) 361
MhlI GDGCHC 2 cut(s) 457, 577
MluCI AATT 3 cut(s) 389, 627, 721
MnlI CCTC 5 cut(s) 77, 277, 301, 468, 595
MslI CAYNNNNRTG 1 cut(s) 653
MspA1I CMGCKG 1 cut(s) 482
MspI CCGG 3 cut(s) 341, 464, 499
MspR9I CCNGG 1 cut(s) 357
Mva1269I GAATGC 3 cut(s) 62, 418, 664
MvaI CCWGG 1 cut(s) 357
MvnI CGCG 2 cut(s) 128, 539
MwoI GCNNNNNNNGC 3 cut(s) 271, 337, 400
NdeII GATC 2 cut(s) 361, 508
NlaIII CATG 5 cut(s) 172, 444, 551, 571, 676
NlaIV GGNNCC 3 cut(s) 288, 363, 456
NmeAIII GCCGAG 1 cut(s) 236
PceI AGGCCT 1 cut(s) 520
PcsI WCGNNNNNNNCGW 1 cut(s) 637
PctI GAATGC 3 cut(s) 62, 418, 664
PfeI GAWTC 1 cut(s) 476
Pfl23II CGTACG 1 cut(s) 540
PkrI GCNGC 6 cut(s) 40, 115, 145, 174, 264, 469
PsiI TTATAA 2 cut(s) 218, 714
Psp6I CCWGG 1 cut(s) 355
PspFI CCCAGC 1 cut(s) 175
PspGI CCWGG 1 cut(s) 355
PspLI CGTACG 1 cut(s) 540
PspN4I GGNNCC 3 cut(s) 288, 363, 456
PsuI RGATCY 1 cut(s) 361
RsaI GTAC 2 cut(s) 110, 542
RsaNI GTAC 2 cut(s) 109, 541
RseI CAYNNNNRTG 1 cut(s) 653
SatI GCNGC 6 cut(s) 39, 114, 144, 173, 263, 468
Sau3AI GATC 2 cut(s) 361, 508
ScrFI CCNGG 1 cut(s) 357
SduI GDGCHC 2 cut(s) 457, 577
SetI ASST 4 cut(s) 77, 165, 565, 643
SmiMI CAYNNNNRTG 1 cut(s) 653
SpeI ACTAGT 1 cut(s) 580
Sse9I AATT 3 cut(s) 389, 627, 721
SseBI AGGCCT 1 cut(s) 520
SsiI CCGC 7 cut(s) 114, 126, 265, 274, 324, 480, 670
SspMI CTAG 1 cut(s) 581
StuI AGGCCT 1 cut(s) 520
StyD4I CCNGG 1 cut(s) 355
TaaI ACNGT 2 cut(s) 88, 545
TaiI ACGT 1 cut(s) 643
TaqI TCGA 2 cut(s) 212, 631
TasI AATT 3 cut(s) 389, 627, 721
TauI GCSGC 1 cut(s) 116
TfiI GAWTC 1 cut(s) 476
TscAI CASTG 2 cut(s) 91, 539
TseI GCWGC 5 cut(s) 38, 143, 172, 262, 467
TspDTI ATGAA 1 cut(s) 671
TspGWI ACGGA 1 cut(s) 279
TspRI CASTG 2 cut(s) 91, 539
VneI GTGCAC 1 cut(s) 573
XmiI GTMKAC 1 cut(s) 293
XspI CTAG 1 cut(s) 581
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.