MD02G1271600.v1.1

Domain of unknown function (DUF313)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0015709
Physical Location & Seq
Reverse (-)
32622339 .. 32623478
1140 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1271600.v1.1.491

Sequence Viewer

Length: 1140 bp
ATGGAGAGGAAGATGACATTGGACGATGTGCGAAAGCTTAAAGTTGAGTCTTTCAACAAACTTCTGGCGGAGGTGAAAGCAGTTGCGAAAAACCGAGATGAAGAACTTGACTTGCTCCAAAAGAGAGTTGTGATTGATTTGGTTGAATTTGAAAGAAAAGCCGCCAAGAAAGTTAGAGAAACGAAGAAGCGCAAAGCTGCTTCTGCTTACTATGATGAAGAAAAACCTACTTCTGCTTCTGCTTCTTATCATCGGAAAGACAGGAAGCTTAATTACGGAAGCAGCAGCCGAAGCAGGAAAGGTGAATACGAATACTATGAGGATTGTGATGAGGAGCTTGAAGAGAAACCAAACACGTCGATGATGATGATGAAGAAGAAGAAGAAGAATCAGAAGCCGACCAAGGAAGCCGCAAGCACGAAACCATCCGCTCCATCAATTCACTCTCCACCGCCGGAGTTGCCTAAAGAATTCAAAGAAAAGATTGCATGCTTGAATGGTTACCAAGTGCAATTGGTGATCCAGAAGAAACTTTTCAAGACTGATTTGAGCAGTGGTCATGATCGTCTCTCAATGCCAGTGAATCAAGTGATTAATAAGAACTTTCTCGGCGAAGACGATGAAAGATTAAACGACAATGGATTTGTATATGTGAAAGTGATAGATCCATGCTTAAAGGTGCATGATAACTTAGGGTTGACCAAGTGGAAAGCTCATAACAACAATAATTTTTCCTATTCCTTAAACAGGGGTTGGAACTCAATTTCTACGGATGAGGAGGTTGGGTTTAAGATAGGGGATATGATTCAGGTTTGGTTTTTTCGAGTTAATGATCCCGAGCTTGCCAAAAAAGACTCTATCCGCTTCGCAGTGGTTAAGCTTAAAAATGATTTTAATGGTAGTACGAGTACTATTGATAGGCAAGAAGCTGCTGAATCGTCAATGACAAAGAAAGATGAGGAGGAGAAAAAATATGCAAACGATTGTAGTAGTAGTTGTAGTGTGAGTGCCAGCCAGGAAGTAATCGAATCATCCACGACCAAGAAGCAGTTGGAGTTTGGAGCCACTAGACAACATGTCAGTATCAAGTTTAGAAGCGTAACACTTGCGAAAATTGAGACGGCTGTTTCTGTTTTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF313

Protein Analysis

380

Amino Acids

43.34

Weight (kDa)

9.06

Isoelectric Point (pI)

37.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
At2g31720-like PF03754 165 - 255 8.4e-13 B3 domain-containing protein At2g31720-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 431
AciI CCGC 6 cut(s) 68, 162, 411, 429, 452, 862
AclWI GGATC 3 cut(s) 514, 659, 827
AcsI RAATTY 2 cut(s) 146, 470
AfaI GTAC 2 cut(s) 904, 910
AfiI CCNNNNNNNGG 1 cut(s) 747
AflIII ACRYGT 2 cut(s) 354, 1075
AgsI TTSAA 7 cut(s) 55, 146, 152, 341, 475, 496, 538
AhdI GACNNNNNGTC 1 cut(s) 1076
AjiI CACGTC 1 cut(s) 357
AjnI CCWGG 1 cut(s) 1014
AjuI GAANNNNNNNTTGG 1 cut(s) 34
AluBI AGCT 8 cut(s) 37, 197, 268, 337, 713, 841, 880, 929
AluI AGCT 8 cut(s) 37, 197, 268, 337, 713, 841, 880, 929
Alw26I GTCTC 2 cut(s) 572, 1112
AlwI GGATC 3 cut(s) 514, 659, 827
Ama87I CYCGRG 1 cut(s) 836
ApeKI GCWGC 4 cut(s) 197, 282, 285, 929
ApoI RAATTY 2 cut(s) 146, 470
ArsI GACNNNNNNTTYG 2 cut(s) 626, 658
AseI ATTAAT 1 cut(s) 594
Asp700I GAANNNNTTC 1 cut(s) 533
AspLEI GCGC 1 cut(s) 192
AsuHPI GGTGA 3 cut(s) 85, 314, 529
AvaI CYCGRG 1 cut(s) 836
BarI GAAGNNNNNNTAC 2 cut(s) 257, 289
BbsI GAAGAC 1 cut(s) 621
BbvI GCAGC 4 cut(s) 184, 294, 297, 916
BccI CCATC 2 cut(s) 433, 442
BceAI ACGGC 1 cut(s) 1137
BciT130I CCWGG 1 cut(s) 1016
BcoDI GTCTC 2 cut(s) 572, 1112
BfaI CTAG 1 cut(s) 1068
BisI GCNGC 6 cut(s) 162, 198, 283, 286, 411, 930
BlsI GCNGC 6 cut(s) 163, 199, 284, 287, 412, 931
BmcAI AGTACT 1 cut(s) 910
Bme1390I CCNGG 1 cut(s) 1016
BmeRI GACNNNNNGTC 1 cut(s) 1076
BmeT110I CYCGRG 1 cut(s) 836
BmgBI CACGTC 1 cut(s) 357
BmiI GGNNCC 1 cut(s) 1063
BmrFI CCNGG 1 cut(s) 1016
BpiI GAAGAC 1 cut(s) 621
BsaJI CCNNGG 1 cut(s) 402
BsaXI ACNNNNNCTCC 2 cut(s) 770, 800
Bsc4I CCNNNNNNNGG 1 cut(s) 747
Bse1I ACTGG 1 cut(s) 578
BseBI CCWGG 1 cut(s) 1016
BseDI CCNNGG 1 cut(s) 402
BseGI GGATG 3 cut(s) 425, 778, 1031
BseLI CCNNNNNNNGG 1 cut(s) 747
BseNI ACTGG 1 cut(s) 578
BseRI GAGGAG 4 cut(s) 347, 791, 974, 977
BseXI GCAGC 4 cut(s) 184, 294, 297, 916
BsiHKCI CYCGRG 1 cut(s) 836
BsiSI CCGG 1 cut(s) 455
BslI CCNNNNNNNGG 1 cut(s) 747
BsmAI GTCTC 2 cut(s) 572, 1112
BsmBI CGTCTC 2 cut(s) 572, 1112
BsoBI CYCGRG 1 cut(s) 836
Bsp143I GATC 4 cut(s) 519, 562, 664, 832
BspACI CCGC 6 cut(s) 68, 162, 411, 429, 452, 862
BspHI TCATGA 1 cut(s) 559
BspLI GGNNCC 1 cut(s) 1063
BspPI GGATC 3 cut(s) 514, 659, 827
BsrBI CCGCTC 1 cut(s) 431
BsrI ACTGG 1 cut(s) 578
BssECI CCNNGG 1 cut(s) 402
BssMI GATC 4 cut(s) 519, 562, 664, 832
BssT1I CCWWGG 1 cut(s) 402
Bst2UI CCWGG 1 cut(s) 1016
Bst6I CTCTTC 1 cut(s) 336
BstC8I GCNNGC 4 cut(s) 415, 490, 843, 1012
BstDEI CTNAG 1 cut(s) 691
BstEII GGTNACC 1 cut(s) 500
BstENI CCTNNNNNAGG 1 cut(s) 745
BstF5I GGATG 3 cut(s) 425, 778, 1031
BstHHI GCGC 1 cut(s) 192
BstKTI GATC 4 cut(s) 522, 565, 667, 835
BstMAI GTCTC 2 cut(s) 572, 1112
BstMBI GATC 4 cut(s) 519, 562, 664, 832
BstMWI GCNNNNNNNGC 3 cut(s) 203, 291, 460
BstNI CCWGG 1 cut(s) 1016
BstNSI RCATGY 2 cut(s) 492, 1079
BstPI GGTNACC 1 cut(s) 500
BstSCI CCNGG 1 cut(s) 1014
BstV1I GCAGC 4 cut(s) 184, 294, 297, 916
BstV2I GAAGAC 1 cut(s) 621
BstX2I RGATCY 1 cut(s) 664
BstYI RGATCY 1 cut(s) 664
BtrI CACGTC 1 cut(s) 357
BtsCI GGATG 3 cut(s) 425, 778, 1031
BtsI GCAGTG 2 cut(s) 559, 876
BtsIMutI CAGTG 3 cut(s) 559, 585, 876
Cac8I GCNNGC 4 cut(s) 415, 490, 843, 1012
CciI TCATGA 1 cut(s) 559
CfoI GCGC 1 cut(s) 192
Csp6I GTAC 2 cut(s) 903, 909
CviAII CATG 5 cut(s) 489, 560, 669, 683, 1076
CviQI GTAC 2 cut(s) 903, 909
DdeI CTNAG 1 cut(s) 691
DpnI GATC 4 cut(s) 521, 564, 666, 834
DpnII GATC 4 cut(s) 519, 562, 664, 832
DriI GACNNNNNGTC 1 cut(s) 1076
Eam1104I CTCTTC 1 cut(s) 336
Eam1105I GACNNNNNGTC 1 cut(s) 1076
EarI CTCTTC 1 cut(s) 336
EciI GGCGGA 1 cut(s) 83
Eco130I CCWWGG 1 cut(s) 402
Eco88I CYCGRG 1 cut(s) 836
Eco91I GGTNACC 1 cut(s) 500
EcoNI CCTNNNNNAGG 1 cut(s) 745
EcoO65I GGTNACC 1 cut(s) 500
EcoRI GAATTC 1 cut(s) 470
EcoRII CCWGG 1 cut(s) 1014
EcoT14I CCWWGG 1 cut(s) 402
ErhI CCWWGG 1 cut(s) 402
Esp3I CGTCTC 2 cut(s) 572, 1112
FaeI CATG 5 cut(s) 492, 563, 672, 686, 1079
FatI CATG 5 cut(s) 488, 559, 668, 682, 1075
Fnu4HI GCNGC 6 cut(s) 162, 198, 283, 286, 411, 930
FokI GGATG 3 cut(s) 412, 785, 1018
Fsp4HI GCNGC 6 cut(s) 162, 198, 283, 286, 411, 930
FspBI CTAG 1 cut(s) 1068
GlaI GCGC 1 cut(s) 191
GluI GCNGC 6 cut(s) 162, 198, 283, 286, 411, 930
HapII CCGG 1 cut(s) 455
HhaI GCGC 1 cut(s) 192
Hin1II CATG 5 cut(s) 492, 563, 672, 686, 1079
Hin6I GCGC 1 cut(s) 190
HinP1I GCGC 1 cut(s) 190
HincII GTYRAC 1 cut(s) 699
HindII GTYRAC 1 cut(s) 699
HindIII AAGCTT 3 cut(s) 35, 266, 878
HinfI GANTC 7 cut(s) 47, 388, 583, 805, 854, 935, 1028
HpaII CCGG 1 cut(s) 455
HphI GGTGA 3 cut(s) 85, 314, 529
Hpy166II GTNNAC 1 cut(s) 699
Hpy188I TCNGA 2 cut(s) 255, 393
Hpy188III TCNNGA 4 cut(s) 523, 538, 560, 836
Hpy8I GTNNAC 1 cut(s) 699
Hpy99I CGWCG 1 cut(s) 361
HpyCH4IV ACGT 1 cut(s) 356
HpyCH4V TGCA 4 cut(s) 488, 511, 682, 977
HpyF10VI GCNNNNNNNGC 3 cut(s) 203, 291, 460
HpyF3I CTNAG 1 cut(s) 691
HpySE526I ACGT 1 cut(s) 356
Hsp92II CATG 5 cut(s) 492, 563, 672, 686, 1079
HspAI GCGC 1 cut(s) 190
Kzo9I GATC 4 cut(s) 519, 562, 664, 832
LmnI GCTCC 4 cut(s) 120, 334, 436, 1061
Lsp1109I GCAGC 4 cut(s) 184, 294, 297, 916
MaeI CTAG 1 cut(s) 1068
MaeII ACGT 1 cut(s) 356
MaeIII GTNAC 2 cut(s) 500, 1099
MalI GATC 4 cut(s) 521, 564, 666, 834
MbiI CCGCTC 1 cut(s) 431
MboI GATC 4 cut(s) 519, 562, 664, 832
MfeI CAATTG 1 cut(s) 512
MflI RGATCY 1 cut(s) 664
MluCI AATT 8 cut(s) 146, 271, 438, 470, 512, 727, 762, 1113
MlyI GAGTC 2 cut(s) 56, 848
MmeI TCCRAC 2 cut(s) 734, 1032
MnlI CCTC 7 cut(s) 64, 313, 325, 769, 772, 952, 955
MroXI GAANNNNTTC 1 cut(s) 533
MslI CAYNNNNRTG 1 cut(s) 359
MspI CCGG 1 cut(s) 455
MspR9I CCNGG 1 cut(s) 1016
MunI CAATTG 1 cut(s) 512
MvaI CCWGG 1 cut(s) 1016
MwoI GCNNNNNNNGC 3 cut(s) 203, 291, 460
NdeII GATC 4 cut(s) 519, 562, 664, 832
NlaIII CATG 5 cut(s) 492, 563, 672, 686, 1079
NlaIV GGNNCC 1 cut(s) 1063
NmeAIII GCCGAG 1 cut(s) 588
NspI RCATGY 2 cut(s) 492, 1079
PaeI GCATGC 1 cut(s) 492
PagI TCATGA 1 cut(s) 559
PciI ACATGT 1 cut(s) 1075
PcsI WCGNNNNNNNCGW 1 cut(s) 615
PdmI GAANNNNTTC 1 cut(s) 533
PfeI GAWTC 5 cut(s) 388, 583, 805, 935, 1028
PkrI GCNGC 6 cut(s) 163, 199, 284, 287, 412, 931
PleI GAGTC 2 cut(s) 55, 848
PpsI GAGTC 2 cut(s) 55, 848
PscI ACATGT 1 cut(s) 1075
PshBI ATTAAT 1 cut(s) 594
Psp6I CCWGG 1 cut(s) 1014
PspEI GGTNACC 1 cut(s) 500
PspGI CCWGG 1 cut(s) 1014
PspN4I GGNNCC 1 cut(s) 1063
PsuI RGATCY 1 cut(s) 664
RsaI GTAC 2 cut(s) 904, 910
RsaNI GTAC 2 cut(s) 903, 909
RseI CAYNNNNRTG 1 cut(s) 359
SatI GCNGC 6 cut(s) 162, 198, 283, 286, 411, 930
Sau3AI GATC 4 cut(s) 519, 562, 664, 832
ScaI AGTACT 1 cut(s) 910
SchI GAGTC 2 cut(s) 56, 848
ScrFI CCNGG 1 cut(s) 1016
SmiMI CAYNNNNRTG 1 cut(s) 359
SphI GCATGC 1 cut(s) 492
Sse9I AATT 8 cut(s) 146, 271, 438, 470, 512, 727, 762, 1113
SsiI CCGC 6 cut(s) 68, 162, 411, 429, 452, 862
SspMI CTAG 1 cut(s) 1068
StyD4I CCNGG 1 cut(s) 1014
StyI CCWWGG 1 cut(s) 402
TaiI ACGT 1 cut(s) 359
TaqI TCGA 3 cut(s) 359, 823, 1026
TasI AATT 8 cut(s) 146, 271, 438, 470, 512, 727, 762, 1113
TatI WGTACW 1 cut(s) 908
TauI GCSGC 2 cut(s) 164, 413
TfiI GAWTC 5 cut(s) 388, 583, 805, 935, 1028
TscAI CASTG 3 cut(s) 559, 585, 876
TseI GCWGC 4 cut(s) 197, 282, 285, 929
TspDTI ATGAA 4 cut(s) 114, 231, 386, 636
TspGWI ACGGA 2 cut(s) 291, 785
TspRI CASTG 3 cut(s) 559, 585, 876
VspI ATTAAT 1 cut(s) 594
XagI CCTNNNNNAGG 1 cut(s) 745
XapI RAATTY 2 cut(s) 146, 470
XceI RCATGY 2 cut(s) 492, 1079
XcmI CCANNNNNNNNNTGG 1 cut(s) 1048
XmnI GAANNNNTTC 1 cut(s) 533
XspI CTAG 1 cut(s) 1068
ZrmI AGTACT 1 cut(s) 910
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.