RLG00000008087

Domain of unknown function (DUF313)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr2
OG0015709
Physical Location & Seq
Forward (+)
25457520 .. 25458677
1158 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000008087

Sequence Viewer

Length: 1038 bp
ATGGGGATCAGCCTTGCAGATTTGAAAAGGAGAGAGTATAGCGAGTCTTTTGATGAACTTTTGGCAAAAGCTAAAGAATTTGCAACAGACCGAGAGGAAGCACTTGAAATTTTGAGAAATTGGATTCTGTATGATTTGAGTTGTAGAAAATCCACAAGAGAAGGAAAAAATTCGACTAATATTGATCCCAAGGAGAAGGCTGAAAATCGACGTGACAGATACAAAATAGTGATCAAGAAGATGAAAGCTAGCTCTACGAAGCCATGCAGTGATATAAGAAAAGACCTGGTCCCTTGCAATGACGAAGATGAAGAAGCTTCTCATCCTTCTTCTGCGAGGAATAATGATCAGCAGATGAAAAGGAAGCAAAGCCCTTATGCATGCTGCTCCAAAATTAGTGAGGATCAATATAAATGTTTCAATAAACTCAAAGAAACTTCTTCTCCTCATTCTTCTACAAGGAAGAAAAGGAAGAGCCAAAGTATTGAAGATCAACATGCATATTACTATACGGTCTATAACTATACAGGTAAAGATGGCAATTTGATGAACTTGATGGAGACGAAGAAGAAGGTGAAGAAGCCACGAGCTTTACCATTTGATGGTCCACCACCAGAGTTGCCTATGGATTTAAGAAACAAGATCGAATCCCTGCATGGCTCCGAGGCCAAACTAGTAATACAGAAGGAGATTACAAATACTGACGTGAACCGCCATCACGATCGTCTGAATATGCCAAGGAACCAAGTACTAACCATGGACGTTCTCAATGAACAGGAGCGAAAAAAGGTTTTTGAACATGGTTTGGAAGTAAGAGTGATAGAACCATGTCTAGAAGAGAGAAACTTACATTTAAGGAAGTGGAATTCGCACAACGGCGGCTATTCCTATGTCTTAAACAGTGGTTGGAAAGATGTTGTTAGTAAGTGTAAGGATCATATTAGGCCTGGTAATACAATTCAGGTTTGGTCATTTCGAGTTGATGATCGCGGAGAGGTCTGGTTTGCCCTTGTGTGCAATGGGCCAAAAAAACTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF313

Protein Analysis

346

Amino Acids

40.22

Weight (kDa)

9.17

Isoelectric Point (pI)

48.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
At2g31720-like PF03754 211 - 309 3.4e-19 B3 domain-containing protein At2g31720-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 602
AccII CGCG 1 cut(s) 990
AciI CCGC 3 cut(s) 712, 879, 990
AclWI GGATC 4 cut(s) 14, 179, 411, 942
AcsI RAATTY 4 cut(s) 77, 108, 169, 865
AfaI GTAC 1 cut(s) 750
AfiI CCNNNNNNNGG 1 cut(s) 602
AgsI TTSAA 5 cut(s) 25, 107, 421, 488, 797
AhlI ACTAGT 1 cut(s) 673
AjiI CACGTC 2 cut(s) 212, 706
AjnI CCWGG 2 cut(s) 285, 946
AluBI AGCT 5 cut(s) 71, 248, 252, 317, 590
AluI AGCT 5 cut(s) 71, 248, 252, 317, 590
Alw26I GTCTC 1 cut(s) 554
AlwI GGATC 4 cut(s) 14, 179, 411, 942
AoxI GGCC 3 cut(s) 666, 944, 1022
ApeKI GCWGC 1 cut(s) 384
ApoI RAATTY 4 cut(s) 77, 108, 169, 865
Asp700I GAANNNNTTC 1 cut(s) 169
AspS9I GGNCC 3 cut(s) 289, 605, 1022
AsuHPI GGTGA 1 cut(s) 586
AsuNHI GCTAGC 1 cut(s) 248
AvaII GGWCC 2 cut(s) 289, 605
BauI CACGAG 1 cut(s) 585
BbvI GCAGC 1 cut(s) 371
BccI CCATC 4 cut(s) 530, 550, 596, 723
BceAI ACGGC 1 cut(s) 892
BciT130I CCWGG 2 cut(s) 287, 948
BclI TGATCA 2 cut(s) 231, 346
BcoDI GTCTC 1 cut(s) 554
BcuI ACTAGT 1 cut(s) 673
BfaI CTAG 3 cut(s) 249, 674, 833
BisI GCNGC 2 cut(s) 385, 880
BlsI GCNGC 2 cut(s) 386, 881
BmcAI AGTACT 1 cut(s) 750
Bme1390I CCNGG 2 cut(s) 287, 948
Bme18I GGWCC 2 cut(s) 289, 605
BmgBI CACGTC 2 cut(s) 212, 706
BmgT120I GGNCC 3 cut(s) 289, 605, 1022
BmiI GGNNCC 3 cut(s) 291, 661, 743
BmrFI CCNGG 2 cut(s) 287, 948
BmtI GCTAGC 1 cut(s) 252
BsaJI CCNNGG 4 cut(s) 189, 663, 737, 756
BsaXI ACNNNNNCTCC 2 cut(s) 427, 457
Bsc4I CCNNNNNNNGG 1 cut(s) 602
Bse3DI GCAATG 2 cut(s) 304, 1024
BseBI CCWGG 2 cut(s) 287, 948
BseDI CCNNGG 4 cut(s) 189, 663, 737, 756
BseGI GGATG 1 cut(s) 322
BseLI CCNNNNNNNGG 1 cut(s) 602
BseMI GCAATG 2 cut(s) 304, 1024
BseRI GAGGAG 1 cut(s) 435
BseXI GCAGC 1 cut(s) 371
Bsh1236I CGCG 1 cut(s) 990
Bsh1285I CGRYCG 1 cut(s) 724
BshFI GGCC 3 cut(s) 668, 946, 1024
BsiEI CGRYCG 1 cut(s) 724
BslFI GGGAC 1 cut(s) 275
BslI CCNNNNNNNGG 1 cut(s) 602
BsmAI GTCTC 1 cut(s) 554
BsmBI CGTCTC 1 cut(s) 554
BsmFI GGGAC 1 cut(s) 275
BsnI GGCC 3 cut(s) 668, 946, 1024
Bsp19I CCATGG 1 cut(s) 756
BspACI CCGC 3 cut(s) 712, 879, 990
BspANI GGCC 3 cut(s) 668, 946, 1024
BspFNI CGCG 1 cut(s) 990
BspLI GGNNCC 3 cut(s) 291, 661, 743
BspOI GCTAGC 1 cut(s) 252
BspPI GGATC 4 cut(s) 14, 179, 411, 942
BspQI GCTCTTC 1 cut(s) 467
BsrDI GCAATG 2 cut(s) 304, 1024
BssECI CCNNGG 4 cut(s) 189, 663, 737, 756
BssSI CACGAG 1 cut(s) 585
BssT1I CCWWGG 3 cut(s) 189, 737, 756
Bst2BI CACGAG 1 cut(s) 585
Bst2UI CCWGG 2 cut(s) 287, 948
Bst4CI ACNGT 3 cut(s) 514, 902, 1035
Bst6I CTCTTC 2 cut(s) 467, 831
BstC8I GCNNGC 2 cut(s) 250, 382
BstDSI CCRYGG 1 cut(s) 756
BstF5I GGATG 1 cut(s) 322
BstFNI CGCG 1 cut(s) 990
BstMAI GTCTC 1 cut(s) 554
BstMCI CGRYCG 1 cut(s) 724
BstNI CCWGG 2 cut(s) 287, 948
BstNSI RCATGY 2 cut(s) 384, 500
BstSCI CCNGG 2 cut(s) 285, 946
BstUI CGCG 1 cut(s) 990
BstV1I GCAGC 1 cut(s) 371
BsuRI GGCC 3 cut(s) 668, 946, 1024
BtgI CCRYGG 1 cut(s) 756
BtrI CACGTC 2 cut(s) 212, 706
BtsCI GGATG 1 cut(s) 322
BtsI GCAGTG 1 cut(s) 274
BtsIMutI CAGTG 2 cut(s) 274, 907
Cac8I GCNNGC 2 cut(s) 250, 382
Cfr13I GGNCC 3 cut(s) 289, 605, 1022
CsiI ACCWGGT 1 cut(s) 285
Csp6I GTAC 1 cut(s) 749
CspCI CAANNNNNGTGG 2 cut(s) 600, 635
CviAII CATG 7 cut(s) 264, 381, 497, 656, 757, 800, 828
CviQI GTAC 1 cut(s) 749
Eam1104I CTCTTC 2 cut(s) 467, 831
EarI CTCTTC 2 cut(s) 467, 831
Eco130I CCWWGG 3 cut(s) 189, 737, 756
Eco147I AGGCCT 1 cut(s) 946
Eco47I GGWCC 2 cut(s) 289, 605
EcoRI GAATTC 1 cut(s) 865
EcoRII CCWGG 2 cut(s) 285, 946
EcoT14I CCWWGG 3 cut(s) 189, 737, 756
EcoT22I ATGCAT 2 cut(s) 382, 502
ErhI CCWWGG 3 cut(s) 189, 737, 756
Esp3I CGTCTC 1 cut(s) 554
FaeI CATG 7 cut(s) 267, 384, 500, 659, 760, 803, 831
FaqI GGGAC 1 cut(s) 275
FatI CATG 7 cut(s) 263, 380, 496, 655, 756, 799, 827
FbaI TGATCA 2 cut(s) 231, 346
Fnu4HI GCNGC 2 cut(s) 385, 880
FokI GGATG 1 cut(s) 309
Fsp4HI GCNGC 2 cut(s) 385, 880
FspBI CTAG 3 cut(s) 249, 674, 833
GluI GCNGC 2 cut(s) 385, 880
HaeIII GGCC 3 cut(s) 668, 946, 1024
Hin1II CATG 7 cut(s) 267, 384, 500, 659, 760, 803, 831
HindIII AAGCTT 1 cut(s) 315
HinfI GANTC 3 cut(s) 44, 124, 647
HphI GGTGA 1 cut(s) 586
Hpy166II GTNNAC 2 cut(s) 608, 709
Hpy188I TCNGA 2 cut(s) 664, 729
Hpy188III TCNNGA 3 cut(s) 235, 719, 833
Hpy8I GTNNAC 2 cut(s) 608, 709
Hpy99I CGWCG 1 cut(s) 213
HpyAV CCTTC 5 cut(s) 155, 190, 336, 565, 679
HpyCH4III ACNGT 3 cut(s) 514, 902, 1035
HpyCH4IV ACGT 3 cut(s) 211, 705, 762
HpyCH4V TGCA 8 cut(s) 17, 83, 267, 297, 380, 500, 655, 1017
HpySE526I ACGT 3 cut(s) 211, 705, 762
Hsp92II CATG 7 cut(s) 267, 384, 500, 659, 760, 803, 831
Ksp22I TGATCA 2 cut(s) 231, 346
LguI GCTCTTC 1 cut(s) 467
LmnI GCTCC 3 cut(s) 392, 665, 778
Lsp1109I GCAGC 1 cut(s) 371
MabI ACCWGGT 1 cut(s) 285
MaeI CTAG 3 cut(s) 249, 674, 833
MaeII ACGT 3 cut(s) 211, 705, 762
MaeIII GTNAC 1 cut(s) 212
MluCI AATT 8 cut(s) 77, 108, 118, 169, 393, 541, 865, 957
MlyI GAGTC 1 cut(s) 53
MmeI TCCRAC 1 cut(s) 887
MnlI CCTC 6 cut(s) 88, 330, 394, 456, 658, 988
Mph1103I ATGCAT 2 cut(s) 382, 502
MroXI GAANNNNTTC 1 cut(s) 169
MseI TTAA 3 cut(s) 632, 854, 896
MspR9I CCNGG 2 cut(s) 287, 948
MvaI CCWGG 2 cut(s) 287, 948
MvnI CGCG 1 cut(s) 990
NcoI CCATGG 1 cut(s) 756
NheI GCTAGC 1 cut(s) 248
NlaIII CATG 7 cut(s) 267, 384, 500, 659, 760, 803, 831
NlaIV GGNNCC 3 cut(s) 291, 661, 743
NmuCI GTSAC 1 cut(s) 212
NsiI ATGCAT 2 cut(s) 382, 502
NspI RCATGY 2 cut(s) 384, 500
PaeI GCATGC 1 cut(s) 384
PceI AGGCCT 1 cut(s) 946
PciSI GCTCTTC 1 cut(s) 467
PdmI GAANNNNTTC 1 cut(s) 169
PfeI GAWTC 2 cut(s) 124, 647
PflFI GACNNNGTC 1 cut(s) 287
PflMI CCANNNNNTGG 1 cut(s) 602
PkrI GCNGC 2 cut(s) 386, 881
Ple19I CGATCG 1 cut(s) 724
PleI GAGTC 1 cut(s) 52
PpsI GAGTC 1 cut(s) 52
Psp6I CCWGG 2 cut(s) 285, 946
PspGI CCWGG 2 cut(s) 285, 946
PspN4I GGNNCC 3 cut(s) 291, 661, 743
PspPI GGNCC 3 cut(s) 289, 605, 1022
PsyI GACNNNGTC 1 cut(s) 287
PvuI CGATCG 1 cut(s) 724
RsaI GTAC 1 cut(s) 750
RsaNI GTAC 1 cut(s) 749
SapI GCTCTTC 1 cut(s) 467
SaqAI TTAA 3 cut(s) 632, 854, 896
SatI GCNGC 2 cut(s) 385, 880
Sau96I GGNCC 3 cut(s) 289, 605, 1022
ScaI AGTACT 1 cut(s) 750
SchI GAGTC 1 cut(s) 53
ScrFI CCNGG 2 cut(s) 287, 948
SexAI ACCWGGT 1 cut(s) 285
SinI GGWCC 2 cut(s) 289, 605
SpeI ACTAGT 1 cut(s) 673
SphI GCATGC 1 cut(s) 384
Sse9I AATT 8 cut(s) 77, 108, 118, 169, 393, 541, 865, 957
SseBI AGGCCT 1 cut(s) 946
SsiI CCGC 3 cut(s) 712, 879, 990
SspI AATATT 1 cut(s) 181
SspMI CTAG 3 cut(s) 249, 674, 833
StuI AGGCCT 1 cut(s) 946
StyD4I CCNGG 2 cut(s) 285, 946
StyI CCWWGG 3 cut(s) 189, 737, 756
TaaI ACNGT 3 cut(s) 514, 902, 1035
TaiI ACGT 3 cut(s) 214, 708, 765
TaqI TCGA 4 cut(s) 173, 208, 645, 976
TaqII GACCGA 1 cut(s) 105
TasI AATT 8 cut(s) 77, 108, 118, 169, 393, 541, 865, 957
TatI WGTACW 1 cut(s) 748
TauI GCSGC 1 cut(s) 882
TfiI GAWTC 2 cut(s) 124, 647
Tru1I TTAA 3 cut(s) 632, 854, 896
Tru9I TTAA 3 cut(s) 632, 854, 896
TscAI CASTG 2 cut(s) 274, 907
TseFI GTSAC 1 cut(s) 212
TseI GCWGC 1 cut(s) 384
Tsp45I GTSAC 1 cut(s) 212
TspDTI ATGAA 6 cut(s) 69, 257, 324, 371, 563, 786
TspRI CASTG 2 cut(s) 274, 907
Tth111I GACNNNGTC 1 cut(s) 287
Van91I CCANNNNNTGG 1 cut(s) 602
VpaK11BI GGWCC 2 cut(s) 289, 605
XapI RAATTY 4 cut(s) 77, 108, 169, 865
XbaI TCTAGA 1 cut(s) 832
XceI RCATGY 2 cut(s) 384, 500
XmnI GAANNNNTTC 1 cut(s) 169
XspI CTAG 3 cut(s) 249, 674, 833
ZrmI AGTACT 1 cut(s) 750
Zsp2I ATGCAT 2 cut(s) 382, 502
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.