Rh4BG191600

Domain of unknown function (DUF313)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4B
OG0015709
Physical Location & Seq
Reverse (-)
33144206 .. 33145628
1423 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4BG191600.1

Sequence Viewer

Length: 1068 bp
ATGGGGATCAGCCTTGAAGATTTGAAAAGGAGAGAGTATAGCGAGTCTTTTGATGAACTTTTAGCAAAAGCTAAAGAATTTGCAACAGACCGAGAGGAAGCACTTAAAATTTTGAGAAATTGGATTCTGTATGATTTGAGTTGTAGAAAATCCACAAGAGAAGAAAAAAAATCGACTAATATTGATCCGAAGGAGAAGGCTGAAAATCGACATGACAGATGCAAAATAGTGATCAAGAAGATGAAAGCTAGCTCTACGAAGCCATGCAATGATATAAGAAAAGATCTGGTCCCTTGCAATGACGAAGATGAAGAAGCTTCTCATCCTTCTTCTGCAAGGAATAATGATCAGCAGATGAAAAGGAAGCAAAGTCCTTATGCATGCTGCTCCAAAATTAGTGAAGATCAAGATAAATGTTTCAATAAACTCAAAGAAGCTTCTTCTCCTCATTCTTCTACAAGGAAGAAAAGGAAGAGCCAAAGTATTGAAGATCAATATGCATATTACTATACGGTCTATAACTATGCAGGGAAAGCTGGCAATTTGATGAACTTGACGGAGAAGAAGAAGAAGGTGAAGAAGCCACAAGCTTTACCATTTGATGGTCCACCACCAGAGTTGCCTGCGGATTTAAGAAACAAGATCGAATCCCTGCATGGCTCCGAGGCCAAACTAGTAATACAGAAGGAGATTACAAATACTGATGTGAACCGTCATCATGATCGTCTGAACATGCCGAGGAACCAAGTACTGACCATGGACGTTCTCAATGAACAAGAGCGAAAAAAGGTCTTTGAACATGGTTTGGAAGTGAGAGTGATACAACCATGTCTAGCAGAGAGAAACTTACATTTAAGGAAGTGGAATTCGCACAACGGCGGCTATTCCTATGTCTTAAACAGTGGTTGGAAAGATGCTGTTAGTAAGTGTGAGGATCGTATTAGGCCTGGTAATACAATTCAGGTTTGGTCATTCCGCGCCGAGTTGATGATCGCGGAGAGGTCTGGTTTGCCCTTGTGTGCCATGGGCCAAAAAAACTGTAACAAGATGAAATTAGCATTTTCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF313

Protein Analysis

355

Amino Acids

41.04

Weight (kDa)

9.25

Isoelectric Point (pI)

53.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
At2g31720-like PF03754 209 - 308 9.9e-18 B3 domain-containing protein At2g31720-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 602
AccII CGCG 2 cut(s) 978, 995
AciI CCGC 4 cut(s) 626, 879, 976, 995
AclWI GGATC 3 cut(s) 14, 179, 942
AcsI RAATTY 3 cut(s) 77, 108, 865
AfaI GTAC 1 cut(s) 750
AfiI CCNNNNNNNGG 1 cut(s) 602
AgsI TTSAA 5 cut(s) 17, 25, 421, 488, 797
AhlI ACTAGT 1 cut(s) 673
AjnI CCWGG 1 cut(s) 946
AluBI AGCT 7 cut(s) 71, 248, 252, 317, 437, 536, 590
AluI AGCT 7 cut(s) 71, 248, 252, 317, 437, 536, 590
AlwI GGATC 3 cut(s) 14, 179, 942
AoxI GGCC 3 cut(s) 666, 944, 1027
ApeKI GCWGC 1 cut(s) 384
ApoI RAATTY 3 cut(s) 77, 108, 865
AspLEI GCGC 1 cut(s) 980
AspS9I GGNCC 3 cut(s) 289, 605, 1027
AsuHPI GGTGA 1 cut(s) 586
AsuNHI GCTAGC 1 cut(s) 248
AvaII GGWCC 2 cut(s) 289, 605
BbvI GCAGC 1 cut(s) 371
BccI CCATC 1 cut(s) 596
BceAI ACGGC 1 cut(s) 892
BciT130I CCWGG 1 cut(s) 948
BclI TGATCA 2 cut(s) 231, 346
BcuI ACTAGT 1 cut(s) 673
BfaI CTAG 4 cut(s) 249, 674, 833, 1066
BglII AGATCT 1 cut(s) 283
BisI GCNGC 2 cut(s) 385, 880
BlsI GCNGC 2 cut(s) 386, 881
BmcAI AGTACT 1 cut(s) 750
Bme1390I CCNGG 1 cut(s) 948
Bme18I GGWCC 2 cut(s) 289, 605
BmgT120I GGNCC 3 cut(s) 289, 605, 1027
BmiI GGNNCC 3 cut(s) 291, 661, 743
BmrFI CCNGG 1 cut(s) 948
BmsI GCATC 2 cut(s) 209, 904
BmtI GCTAGC 1 cut(s) 252
BsaJI CCNNGG 4 cut(s) 663, 737, 756, 1023
Bsc4I CCNNNNNNNGG 1 cut(s) 602
Bse3DI GCAATG 2 cut(s) 274, 304
BseBI CCWGG 1 cut(s) 948
BseDI CCNNGG 4 cut(s) 663, 737, 756, 1023
BseGI GGATG 1 cut(s) 322
BseLI CCNNNNNNNGG 1 cut(s) 602
BseMI GCAATG 2 cut(s) 274, 304
BseRI GAGGAG 1 cut(s) 435
BseXI GCAGC 1 cut(s) 371
Bsh1236I CGCG 2 cut(s) 978, 995
BshFI GGCC 3 cut(s) 668, 946, 1029
BslFI GGGAC 1 cut(s) 275
BslI CCNNNNNNNGG 1 cut(s) 602
BsmFI GGGAC 1 cut(s) 275
BsnI GGCC 3 cut(s) 668, 946, 1029
Bsp19I CCATGG 2 cut(s) 756, 1023
BspACI CCGC 4 cut(s) 626, 879, 976, 995
BspANI GGCC 3 cut(s) 668, 946, 1029
BspFNI CGCG 2 cut(s) 978, 995
BspHI TCATGA 1 cut(s) 718
BspLI GGNNCC 3 cut(s) 291, 661, 743
BspOI GCTAGC 1 cut(s) 252
BspPI GGATC 3 cut(s) 14, 179, 942
BspQI GCTCTTC 1 cut(s) 467
BsrDI GCAATG 2 cut(s) 274, 304
BssECI CCNNGG 4 cut(s) 663, 737, 756, 1023
BssT1I CCWWGG 2 cut(s) 756, 1023
Bst2UI CCWGG 1 cut(s) 948
Bst4CI ACNGT 4 cut(s) 514, 713, 902, 1040
Bst6I CTCTTC 1 cut(s) 467
BstC8I GCNNGC 4 cut(s) 250, 382, 538, 624
BstDSI CCRYGG 2 cut(s) 756, 1023
BstF5I GGATG 1 cut(s) 322
BstFNI CGCG 2 cut(s) 978, 995
BstHHI GCGC 1 cut(s) 980
BstMWI GCNNNNNNNGC 1 cut(s) 533
BstNI CCWGG 1 cut(s) 948
BstNSI RCATGY 2 cut(s) 384, 736
BstSCI CCNGG 1 cut(s) 946
BstUI CGCG 2 cut(s) 978, 995
BstV1I GCAGC 1 cut(s) 371
BstX2I RGATCY 1 cut(s) 283
BstYI RGATCY 1 cut(s) 283
BsuRI GGCC 3 cut(s) 668, 946, 1029
BtgI CCRYGG 2 cut(s) 756, 1023
BtsCI GGATG 1 cut(s) 322
BtsIMutI CAGTG 1 cut(s) 907
Cac8I GCNNGC 4 cut(s) 250, 382, 538, 624
CciI TCATGA 1 cut(s) 718
CfoI GCGC 1 cut(s) 980
Cfr13I GGNCC 3 cut(s) 289, 605, 1027
Csp6I GTAC 1 cut(s) 749
CspCI CAANNNNNGTGG 2 cut(s) 600, 635
CviQI GTAC 1 cut(s) 749
Eam1104I CTCTTC 1 cut(s) 467
EarI CTCTTC 1 cut(s) 467
Eco130I CCWWGG 2 cut(s) 756, 1023
Eco147I AGGCCT 1 cut(s) 946
Eco47I GGWCC 2 cut(s) 289, 605
EcoRI GAATTC 1 cut(s) 865
EcoRII CCWGG 1 cut(s) 946
EcoT14I CCWWGG 2 cut(s) 756, 1023
EcoT22I ATGCAT 2 cut(s) 382, 502
ErhI CCWWGG 2 cut(s) 756, 1023
FaqI GGGAC 1 cut(s) 275
FbaI TGATCA 2 cut(s) 231, 346
Fnu4HI GCNGC 2 cut(s) 385, 880
FokI GGATG 1 cut(s) 309
Fsp4HI GCNGC 2 cut(s) 385, 880
FspBI CTAG 4 cut(s) 249, 674, 833, 1066
GlaI GCGC 1 cut(s) 979
GluI GCNGC 2 cut(s) 385, 880
HaeIII GGCC 3 cut(s) 668, 946, 1029
HhaI GCGC 1 cut(s) 980
Hin6I GCGC 1 cut(s) 978
HinP1I GCGC 1 cut(s) 978
HindIII AAGCTT 3 cut(s) 315, 435, 588
HinfI GANTC 3 cut(s) 44, 124, 647
HphI GGTGA 1 cut(s) 586
Hpy166II GTNNAC 2 cut(s) 608, 709
Hpy188I TCNGA 3 cut(s) 189, 664, 729
Hpy188III TCNNGA 3 cut(s) 235, 407, 719
Hpy8I GTNNAC 2 cut(s) 608, 709
HpyAV CCTTC 5 cut(s) 184, 190, 336, 565, 679
HpyCH4III ACNGT 4 cut(s) 514, 713, 902, 1040
HpyCH4IV ACGT 1 cut(s) 762
HpyCH4V TGCA 9 cut(s) 83, 222, 267, 297, 335, 380, 500, 527, 655
HpyF10VI GCNNNNNNNGC 1 cut(s) 533
HpySE526I ACGT 1 cut(s) 762
HspAI GCGC 1 cut(s) 978
Ksp22I TGATCA 2 cut(s) 231, 346
LguI GCTCTTC 1 cut(s) 467
LmnI GCTCC 2 cut(s) 392, 665
Lsp1109I GCAGC 1 cut(s) 371
LweI GCATC 2 cut(s) 209, 904
MaeI CTAG 4 cut(s) 249, 674, 833, 1066
MaeII ACGT 1 cut(s) 762
MaeIII GTNAC 1 cut(s) 1040
MflI RGATCY 1 cut(s) 283
MluCI AATT 8 cut(s) 77, 108, 118, 393, 541, 865, 957, 1052
MlyI GAGTC 1 cut(s) 53
MmeI TCCRAC 1 cut(s) 887
MnlI CCTC 6 cut(s) 88, 456, 658, 732, 925, 993
Mph1103I ATGCAT 2 cut(s) 382, 502
MseI TTAA 4 cut(s) 105, 632, 854, 896
MspR9I CCNGG 1 cut(s) 948
MvaI CCWGG 1 cut(s) 948
MvnI CGCG 2 cut(s) 978, 995
MwoI GCNNNNNNNGC 1 cut(s) 533
NcoI CCATGG 2 cut(s) 756, 1023
NheI GCTAGC 1 cut(s) 248
NlaIV GGNNCC 3 cut(s) 291, 661, 743
NmeAIII GCCGAG 2 cut(s) 762, 1006
NsiI ATGCAT 2 cut(s) 382, 502
NspI RCATGY 2 cut(s) 384, 736
PaeI GCATGC 1 cut(s) 384
PagI TCATGA 1 cut(s) 718
PceI AGGCCT 1 cut(s) 946
PciSI GCTCTTC 1 cut(s) 467
PfeI GAWTC 2 cut(s) 124, 647
PflMI CCANNNNNTGG 1 cut(s) 602
PkrI GCNGC 2 cut(s) 386, 881
PleI GAGTC 1 cut(s) 52
PpsI GAGTC 1 cut(s) 52
Psp6I CCWGG 1 cut(s) 946
PspGI CCWGG 1 cut(s) 946
PspN4I GGNNCC 3 cut(s) 291, 661, 743
PspPI GGNCC 3 cut(s) 289, 605, 1027
PsuI RGATCY 1 cut(s) 283
RsaI GTAC 1 cut(s) 750
RsaNI GTAC 1 cut(s) 749
SapI GCTCTTC 1 cut(s) 467
SaqAI TTAA 4 cut(s) 105, 632, 854, 896
SatI GCNGC 2 cut(s) 385, 880
Sau96I GGNCC 3 cut(s) 289, 605, 1027
ScaI AGTACT 1 cut(s) 750
SchI GAGTC 1 cut(s) 53
ScrFI CCNGG 1 cut(s) 948
SfaNI GCATC 2 cut(s) 209, 904
SinI GGWCC 2 cut(s) 289, 605
SpeI ACTAGT 1 cut(s) 673
SphI GCATGC 1 cut(s) 384
Sse9I AATT 8 cut(s) 77, 108, 118, 393, 541, 865, 957, 1052
SseBI AGGCCT 1 cut(s) 946
SsiI CCGC 4 cut(s) 626, 879, 976, 995
SspI AATATT 1 cut(s) 181
SspMI CTAG 4 cut(s) 249, 674, 833, 1066
StuI AGGCCT 1 cut(s) 946
StyD4I CCNGG 1 cut(s) 946
StyI CCWWGG 2 cut(s) 756, 1023
TaaI ACNGT 4 cut(s) 514, 713, 902, 1040
TaiI ACGT 1 cut(s) 765
TaqI TCGA 3 cut(s) 173, 208, 645
TaqII GACCGA 1 cut(s) 105
TasI AATT 8 cut(s) 77, 108, 118, 393, 541, 865, 957, 1052
TatI WGTACW 1 cut(s) 748
TauI GCSGC 1 cut(s) 882
TfiI GAWTC 2 cut(s) 124, 647
Tru1I TTAA 4 cut(s) 105, 632, 854, 896
Tru9I TTAA 4 cut(s) 105, 632, 854, 896
TscAI CASTG 1 cut(s) 907
TseI GCWGC 1 cut(s) 384
TspDTI ATGAA 7 cut(s) 69, 257, 324, 371, 563, 786, 1064
TspGWI ACGGA 1 cut(s) 572
TspRI CASTG 1 cut(s) 907
Van91I CCANNNNNTGG 1 cut(s) 602
VpaK11BI GGWCC 2 cut(s) 289, 605
XapI RAATTY 3 cut(s) 77, 108, 865
XceI RCATGY 2 cut(s) 384, 736
XspI CTAG 4 cut(s) 249, 674, 833, 1066
ZrmI AGTACT 1 cut(s) 750
Zsp2I ATGCAT 2 cut(s) 382, 502
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.