RLG00000025856

B3 domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0015709
Physical Location & Seq
Forward (+)
48699995 .. 48700408
414 bp
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UTR
Exon/CDS
Intron
RLM00000025856

Sequence Viewer

Length: 414 bp
ATGGAAAACCCTGCAGAAGTGTACAACATGATCGAACTCTTGCTGCAGCCTGCCCATGTCAGATTGGTGATGCAGAAGCAGCTTTCTTTTTCCGACGTGACGACGAGTCTCAACCGTCTAGCAATATCAGAGAATCAAATGAAAGTTGAAAAGTTTCTTAATGAGCAAGAACTGGGGGTTATCAGTGCACAAGTAGGCGTCCCGGTGCGCGTGATAGGACCATTATTTGAGTCGAATTTAGAGTTGAGGAAGTGGAATCTCATGACTGAAGACAATTCAGCCTTATTCTATGTGTTAATTAACGGTTGGGCAGAATTTGTTCGAAGGAATCATCTAAGGCGAAGTGATACAATTCAGCTGTGGTCGTTCCGTATTAACGGCGACCTATGCTTCGCAATTATTAAACTCGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF313

Protein Analysis

138

Amino Acids

15.88

Weight (kDa)

5.71

Isoelectric Point (pI)

43.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
At2g31720-like PF03754 18 - 107 3.4e-12 B3 domain-containing protein At2g31720-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 210
AcsI RAATTY 2 cut(s) 235, 314
AcuI CTGAAG 1 cut(s) 288
AcyI GRCGYC 1 cut(s) 198
AfaI GTAC 1 cut(s) 23
AgsI TTSAA 1 cut(s) 149
AhdI GACNNNNNGTC 1 cut(s) 105
AjiI CACGTC 1 cut(s) 97
AluBI AGCT 2 cut(s) 82, 358
AluI AGCT 2 cut(s) 82, 358
Alw21I GWGCWC 1 cut(s) 190
Alw26I GTCTC 1 cut(s) 113
Alw44I GTGCAC 1 cut(s) 186
ApaLI GTGCAC 1 cut(s) 186
ApeKI GCWGC 3 cut(s) 43, 46, 79
ApoI RAATTY 2 cut(s) 235, 314
ArsI GACNNNNNNTTYG 2 cut(s) 374, 406
Asp700I GAANNNNTTC 2 cut(s) 153, 318
AspLEI GCGC 1 cut(s) 210
AspS9I GGNCC 1 cut(s) 218
AsuC2I CCSGG 1 cut(s) 203
AsuHPI GGTGA 1 cut(s) 79
AsuII TTCGAA 1 cut(s) 322
AvaII GGWCC 1 cut(s) 218
BaeGI GKGCMC 1 cut(s) 190
BbsI GAAGAC 1 cut(s) 276
Bbv12I GWGCWC 1 cut(s) 190
BbvI GCAGC 3 cut(s) 30, 58, 91
BceAI ACGGC 1 cut(s) 394
BcnI CCSGG 1 cut(s) 203
BcoDI GTCTC 1 cut(s) 113
BfaI CTAG 1 cut(s) 119
BfmI CTRYAG 2 cut(s) 12, 44
BisI GCNGC 3 cut(s) 44, 47, 80
BlsI GCNGC 3 cut(s) 45, 48, 81
Bme1390I CCNGG 1 cut(s) 203
Bme18I GGWCC 1 cut(s) 218
BmeRI GACNNNNNGTC 1 cut(s) 105
BmgBI CACGTC 1 cut(s) 97
BmgT120I GGNCC 1 cut(s) 218
BmrFI CCNGG 1 cut(s) 203
BmrI ACTGGG 1 cut(s) 182
BmsI GCATC 1 cut(s) 60
BmuI ACTGGG 1 cut(s) 182
BpiI GAAGAC 1 cut(s) 276
Bpu14I TTCGAA 1 cut(s) 322
BpuMI CCSGG 1 cut(s) 203
BsaHI GRCGYC 1 cut(s) 198
Bse1I ACTGG 1 cut(s) 177
BseNI ACTGG 1 cut(s) 177
BseSI GKGCMC 1 cut(s) 190
BseXI GCAGC 3 cut(s) 30, 58, 91
Bsh1236I CGCG 1 cut(s) 210
BsiHKAI GWGCWC 1 cut(s) 190
BsiSI CCGG 1 cut(s) 203
BslFI GGGAC 1 cut(s) 185
BsmAI GTCTC 1 cut(s) 113
BsmFI GGGAC 1 cut(s) 185
Bsp119I TTCGAA 1 cut(s) 322
Bsp1286I GDGCHC 1 cut(s) 190
Bsp1407I TGTACA 1 cut(s) 21
Bsp143I GATC 1 cut(s) 30
BspFNI CGCG 1 cut(s) 210
BspHI TCATGA 1 cut(s) 261
BspMAI CTGCAG 2 cut(s) 16, 48
BspT104I TTCGAA 1 cut(s) 322
BsrGI TGTACA 1 cut(s) 21
BsrI ACTGG 1 cut(s) 177
BssMI GATC 1 cut(s) 30
BssNI GRCGYC 1 cut(s) 198
Bst4CI ACNGT 2 cut(s) 116, 305
BstACI GRCGYC 1 cut(s) 198
BstAUI TGTACA 1 cut(s) 21
BstBI TTCGAA 1 cut(s) 322
BstC8I GCNNGC 1 cut(s) 51
BstDEI CTNAG 1 cut(s) 335
BstFNI CGCG 1 cut(s) 210
BstHHI GCGC 1 cut(s) 210
BstKTI GATC 1 cut(s) 33
BstMAI GTCTC 1 cut(s) 113
BstMBI GATC 1 cut(s) 30
BstMWI GCNNNNNNNGC 2 cut(s) 79, 387
BstSCI CCNGG 1 cut(s) 201
BstSFI CTRYAG 2 cut(s) 12, 44
BstSLI GKGCMC 1 cut(s) 190
BstUI CGCG 1 cut(s) 210
BstV1I GCAGC 3 cut(s) 30, 58, 91
BstV2I GAAGAC 1 cut(s) 276
BtrI CACGTC 1 cut(s) 97
BtsIMutI CAGTG 1 cut(s) 190
Cac8I GCNNGC 1 cut(s) 51
CciI TCATGA 1 cut(s) 261
CfoI GCGC 1 cut(s) 210
Cfr13I GGNCC 1 cut(s) 218
CseI GACGC 1 cut(s) 187
Csp6I GTAC 1 cut(s) 22
CviAII CATG 3 cut(s) 28, 56, 262
CviJI RGCY 4 cut(s) 49, 82, 281, 358
CviKI_1 RGCY 4 cut(s) 49, 82, 281, 358
CviQI GTAC 1 cut(s) 22
DdeI CTNAG 1 cut(s) 335
DpnI GATC 1 cut(s) 32
DpnII GATC 1 cut(s) 30
DriI GACNNNNNGTC 1 cut(s) 105
Eam1105I GACNNNNNGTC 1 cut(s) 105
Eco47I GGWCC 1 cut(s) 218
Eco57I CTGAAG 1 cut(s) 288
FaeI CATG 3 cut(s) 31, 59, 265
FaiI YATR 5 cut(s) 29, 57, 263, 291, 388
FaqI GGGAC 1 cut(s) 185
FatI CATG 3 cut(s) 27, 55, 261
Fnu4HI GCNGC 3 cut(s) 44, 47, 80
Fsp4HI GCNGC 3 cut(s) 44, 47, 80
FspBI CTAG 1 cut(s) 119
GlaI GCGC 1 cut(s) 209
GluI GCNGC 3 cut(s) 44, 47, 80
HapII CCGG 1 cut(s) 203
HgaI GACGC 1 cut(s) 187
HhaI GCGC 1 cut(s) 210
Hin1I GRCGYC 1 cut(s) 198
Hin1II CATG 3 cut(s) 31, 59, 265
Hin6I GCGC 1 cut(s) 208
HinP1I GCGC 1 cut(s) 208
HinfI GANTC 5 cut(s) 106, 133, 230, 256, 328
HpaII CCGG 1 cut(s) 203
HphI GGTGA 1 cut(s) 79
Hpy166II GTNNAC 2 cut(s) 22, 188
Hpy188I TCNGA 3 cut(s) 62, 94, 130
Hpy188III TCNNGA 1 cut(s) 262
Hpy8I GTNNAC 2 cut(s) 22, 188
Hpy99I CGWCG 2 cut(s) 98, 106
HpyAV CCTTC 1 cut(s) 318
HpyCH4III ACNGT 2 cut(s) 116, 305
HpyCH4IV ACGT 1 cut(s) 96
HpyCH4V TGCA 4 cut(s) 14, 46, 73, 188
HpyF10VI GCNNNNNNNGC 2 cut(s) 79, 387
HpyF3I CTNAG 1 cut(s) 335
HpySE526I ACGT 1 cut(s) 96
Hsp92I GRCGYC 1 cut(s) 198
Hsp92II CATG 3 cut(s) 31, 59, 265
HspAI GCGC 1 cut(s) 208
Kzo9I GATC 1 cut(s) 30
LpnPI CCDG 4 cut(s) 24, 63, 158, 216
Lsp1109I GCAGC 3 cut(s) 30, 58, 91
LweI GCATC 1 cut(s) 60
MaeI CTAG 1 cut(s) 119
MaeII ACGT 1 cut(s) 96
MaeIII GTNAC 1 cut(s) 97
MalI GATC 1 cut(s) 32
MboI GATC 1 cut(s) 30
MboII GAAGA 1 cut(s) 281
MhlI GDGCHC 1 cut(s) 190
MluCI AATT 6 cut(s) 235, 274, 297, 314, 351, 396
MlyI GAGTC 2 cut(s) 115, 239
MmeI TCCRAC 1 cut(s) 117
MnlI CCTC 1 cut(s) 240
MroXI GAANNNNTTC 2 cut(s) 153, 318
MseI TTAA 5 cut(s) 159, 296, 300, 375, 402
MspA1I CMGCKG 1 cut(s) 358
MspI CCGG 1 cut(s) 203
MspR9I CCNGG 1 cut(s) 203
MvnI CGCG 1 cut(s) 210
MwoI GCNNNNNNNGC 2 cut(s) 79, 387
NciI CCSGG 1 cut(s) 203
NdeII GATC 1 cut(s) 30
NlaIII CATG 3 cut(s) 31, 59, 265
NmuCI GTSAC 1 cut(s) 97
NspV TTCGAA 1 cut(s) 322
PacI TTAATTAA 1 cut(s) 300
PagI TCATGA 1 cut(s) 261
PdmI GAANNNNTTC 2 cut(s) 153, 318
PfeI GAWTC 3 cut(s) 133, 256, 328
PkrI GCNGC 3 cut(s) 45, 48, 81
PleI GAGTC 2 cut(s) 114, 238
PpsI GAGTC 2 cut(s) 114, 238
PspPI GGNCC 1 cut(s) 218
PstI CTGCAG 2 cut(s) 16, 48
PvuII CAGCTG 1 cut(s) 358
RsaI GTAC 1 cut(s) 23
RsaNI GTAC 1 cut(s) 22
SaqAI TTAA 5 cut(s) 159, 296, 300, 375, 402
SatI GCNGC 3 cut(s) 44, 47, 80
Sau3AI GATC 1 cut(s) 30
Sau96I GGNCC 1 cut(s) 218
SchI GAGTC 2 cut(s) 115, 239
ScrFI CCNGG 1 cut(s) 203
SduI GDGCHC 1 cut(s) 190
SetI ASST 4 cut(s) 84, 99, 360, 387
SfaNI GCATC 1 cut(s) 60
SfcI CTRYAG 2 cut(s) 12, 44
SfuI TTCGAA 1 cut(s) 322
SinI GGWCC 1 cut(s) 218
Sse9I AATT 6 cut(s) 235, 274, 297, 314, 351, 396
SspMI CTAG 1 cut(s) 119
StyD4I CCNGG 1 cut(s) 201
TaaI ACNGT 2 cut(s) 116, 305
TaiI ACGT 1 cut(s) 99
TaqI TCGA 4 cut(s) 33, 233, 322, 408
TasI AATT 6 cut(s) 235, 274, 297, 314, 351, 396
TatI WGTACW 1 cut(s) 21
TfiI GAWTC 3 cut(s) 133, 256, 328
Tru1I TTAA 5 cut(s) 159, 296, 300, 375, 402
Tru9I TTAA 5 cut(s) 159, 296, 300, 375, 402
TscAI CASTG 1 cut(s) 190
TseFI GTSAC 1 cut(s) 97
TseI GCWGC 3 cut(s) 43, 46, 79
Tsp45I GTSAC 1 cut(s) 97
TspDTI ATGAA 1 cut(s) 155
TspGWI ACGGA 1 cut(s) 359
TspRI CASTG 1 cut(s) 190
VneI GTGCAC 1 cut(s) 186
VpaK11BI GGWCC 1 cut(s) 218
XapI RAATTY 2 cut(s) 235, 314
XmnI GAANNNNTTC 2 cut(s) 153, 318
XspI CTAG 1 cut(s) 119
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.