Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr02

Orthogroup ID

OG0012915

Physical Location & Seq

Genomic Coordinates Forward (+)

35467566 .. 35469068

Gene Structure 1503 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD02G1299900.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1503 bp

ATGGGGTACCTTTCTTGCCGAGCTGACTCAGCCATCTCCGTCGCCAATTCCCAAACCTCATCGTCCAAGACCACCGCCACCGCCACCGCTGAAAAGCCTATCAAGATCCAACACTTCGACTACAGTGATCTTGTGGCCGCCACCAATGGCTTCTCCGAACAGAAGCTTTTGGGCAAAGGCAGCCATGGCTATGTCTACAAAGCTATGCTCCGCGGCCGTCTAGTTGCCATCAAAAGACCCTCCAGAGCTCACAGCCGGACTCTCCGACCCAACTCGTGTTCTGCGCCGGAGACCGCCAACGAGGTCGACAACGAGATCGAGATCTTGTCTAAGATTCAGAGTCCGAGGCTGGTCAATCTTTTGGGGTTTTCGAATGATTCGAAAGACAGGCTTTTGGTGGTGGAGTTCATGAGCAACGGTACGCTGTACGATGTTTTACATTCGAGTACGAATCGGCCTCCGAATTGGGGTCGTAGGATTAGATTAGCTTTGCAAACTGCCAAGGCAATTGACATTTTACATTCATCGAGCCCACCTGTAATTCATAGAGATATTAAGTCTGCAAATGTTTTGATTGATCGTAGTTACAATGCCCGGTTGGGGGATTTTGGATTGGCTCTGAGGTACTGCCTGTTCGACGAATATAGGCTCCGGTCGACCCCTCCGGCGGGGACGATGGGGTACTTGGACCCTTGCTATGTGACCCCTGACAATTTGAGTACTAAAACTGATGTTTTTAGTTTTGGGATTTTGTTGTTGGAGATTTTAAGTGGTAGGAAGGCTATAGATGTTGGGCATTCTCCGCCTTCGATTGTGGATTGGGCTATCCCGCTTATAAAGCGAGGGAAGCTGATAACGGTTTATGATCCGCGAATTGTACCTCCTAAGGACCCTGTTGTGAGGAAGCAATTAGCTGTCATTGCAGCCAAGTGTGTGAGGTCTTGTAGGGAGCGTAGGCCAACGATGAAGGAGGTTGTGGTTTGGCTTACCGGGTTGAGTAAACTGGTTCCCCTGCATTCGTGGAATGGTTTTAACAATCCGTGTACAATGGTAGAGACCGTGGGGCGGCCTGTTGAATCGAGGAATGCACATTTGAGTTCGAAGCAAGAGGTTGTTGGGGAAGAGAATTTGGAAGCCTTGGAAGCTAAGTTGACTAGGCGAGTATTGAGGAATTCGCAGAGAGTTTATTCAGATTTGGGGTTTAGTAGCAATCTGATGGAGCTTATGGCAGGTACTGATGGGGAGCCTGACTTTCATGGTAATGCAGATGGAGATGATCGCAGTTCAAAATCAGCGAATTGGGTTTCTAGATTTGGTAGCGGACGATACATTGGCAGGATTAATAAATCAACTCCTGGTAACGGGAAACAAGTCCCTGATTTGATGCAAAATCATTCTTTTGGGAAAAGGTCGTTTACATGTTCAAGGAGAGATGATGCAATGCCTCATGTGAGCGGCAGTTCCCGTGCATCTGCTGGTATACAGCTTGCTGCAGAAATTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004675 GO:0004888 GO:0005575 GO:0005623 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0007154 GO:0007165 GO:0007166 GO:0007167 GO:0007178 GO:0008150 GO:0008152 GO:0009987 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019199 GO:0019538 GO:0023052 GO:0036211 GO:0038023 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044464 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0060089 GO:0065007 GO:0071704 GO:0071944 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

ko04010 ko04064 ko04620 ko04621 ko04624 ko04722 ko05133 ko05140 ko05142 ko05145 ko05152 ko05162 ko05164 ko05169 map04010 map04064 map04620 map04621 map04624 map04722 map05133 map05140 map05142 map05145 map05152 map05162 map05164 map05169

Pfam Domains

Protein Families

Pkinase Pkinase_Tyr

Protein Analysis

501

Amino Acids

55.11

Weight (kDa)

9.57

Isoelectric Point (pI)

43.99

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Pkinase	PF00069	52 - 326	1.1e-36	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	54 - 326	1e-34	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0012915)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G51990
fragaria_vesca	FvH4_7g01890
malus_domestica	MD02G1299900.v1.1 MD07G1025000.v1.1
prunus_persica	Prupe.2G024700_v2.0.a1
pyrus_communis	pycom02g25190 pycom03g05450 pycom07g01930
rosa_chinensis	RchiOBHm_Chr1g0318491
rosa_laevigata	RLG00000030533
rosa_multiflora	Rmu_co8489629.1_g000001
rosa_roxburghii	Rroxscaffold_4G00329110
rosa_rugosa	Rorug01G0021700
rosa_samantha	Rh1AG034100 Rh1BG030200 Rh1CG033600 Rh1DG037000
rosa_wichuraiana	Rw1G002710

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	836
Acc36I	ACCTGC	1 cut(s)	1220
Acc65I	GGTACC	1 cut(s)	6
AccB1I	GGYRCC	1 cut(s)	6
AccBSI	CCGCTC	1 cut(s)	1455
AccI	GTMKAC	4 cut(s)	195, 306, 656, 1480
AccII	CGCG	2 cut(s)	213, 871
AclWI	GGATC	2 cut(s)	100, 860
AcoI	YGGCCR	2 cut(s)	135, 214
AcsI	RAATTY	3 cut(s)	1126, 1171, 1497
AfiI	CCNNNNNNNGG	7 cut(s)	467, 600, 601, 667, 668, 1065, 1361
AflIII	ACRYGT	1 cut(s)	1418
AgsI	TTSAA	3 cut(s)	1076, 1287, 1425
AjnI	CCWGG	1 cut(s)	1354
Alw21I	GWGCWC	1 cut(s)	250
Alw26I	GTCTC	2 cut(s)	284, 1049
AlwI	GGATC	2 cut(s)	100, 860
AlwNI	CAGNNNCTG	1 cut(s)	1235
AoxI	GGCC	5 cut(s)	135, 214, 455, 956, 1067
ApeKI	GCWGC	3 cut(s)	180, 923, 1490
ApoI	RAATTY	3 cut(s)	1126, 1171, 1497
AseI	ATTAAT	1 cut(s)	1341
Asp718I	GGTACC	1 cut(s)	6
AspLEI	GCGC	1 cut(s)	286
AspS9I	GGNCC	2 cut(s)	688, 889
AsuC2I	CCSGG	2 cut(s)	595, 991
AsuII	TTCGAA	3 cut(s)	371, 380, 1100
AvaII	GGWCC	2 cut(s)	688, 889
AxyI	CCTNAGG	1 cut(s)	885
BanI	GGYRCC	1 cut(s)	6
BanII	GRGCYC	2 cut(s)	250, 533
BauI	CACGAG	1 cut(s)	274
Bbv12I	GWGCWC	1 cut(s)	250
BbvI	GCAGC	3 cut(s)	192, 935, 1477
BccI	CCATC	6 cut(s)	41, 236, 670, 1210, 1232, 1262
BceAI	ACGGC	1 cut(s)	201
BciT130I	CCWGG	1 cut(s)	1356
BcnI	CCSGG	2 cut(s)	595, 991
BcoDI	GTCTC	2 cut(s)	284, 1049
BfaI	CTAG	3 cut(s)	221, 1155, 1308
BfmI	CTRYAG	3 cut(s)	121, 783, 1491
BfuAI	ACCTGC	1 cut(s)	1220
BglII	AGATCT	1 cut(s)	321
BisI	GCNGC	7 cut(s)	138, 181, 214, 924, 1067, 1456, 1491
BlsI	GCNGC	7 cut(s)	139, 182, 215, 925, 1068, 1457, 1492
BmcAI	AGTACT	1 cut(s)	721
Bme1390I	CCNGG	3 cut(s)	595, 991, 1356
Bme18I	GGWCC	2 cut(s)	688, 889
BmgT120I	GGNCC	2 cut(s)	688, 889
BmiI	GGNNCC	6 cut(s)	8, 650, 690, 891, 1008, 1245
BmrFI	CCNGG	3 cut(s)	595, 991, 1356
BmsI	GCATC	3 cut(s)	1374, 1426, 1478
BpmI	CTGGAG	1 cut(s)	226
Bpu14I	TTCGAA	3 cut(s)	371, 380, 1100
BpuMI	CCSGG	2 cut(s)	595, 991
BsaBI	GATNNNNATC	1 cut(s)	320
BsaI	GGTCTC	2 cut(s)	284, 1049
BsaJI	CCNNGG	6 cut(s)	184, 211, 344, 501, 1059, 1137
BsaWI	WCCGGW	1 cut(s)	651
Bsc4I	CCNNNNNNNGG	7 cut(s)	467, 600, 601, 667, 668, 1065, 1361
Bse1I	ACTGG	1 cut(s)	1008
Bse21I	CCTNAGG	1 cut(s)	885
Bse3DI	GCAATG	2 cut(s)	918, 1446
Bse8I	GATNNNNATC	1 cut(s)	320
BseBI	CCWGG	1 cut(s)	1356
BseDI	CCNNGG	6 cut(s)	184, 211, 344, 501, 1059, 1137
BseJI	GATNNNNATC	1 cut(s)	320
BseLI	CCNNNNNNNGG	7 cut(s)	467, 600, 601, 667, 668, 1065, 1361
BseMI	GCAATG	2 cut(s)	918, 1446
BseMII	CTCAG	2 cut(s)	42, 611
BseNI	ACTGG	1 cut(s)	1008
BseX3I	CGGCCG	1 cut(s)	214
BseXI	GCAGC	3 cut(s)	192, 935, 1477
Bsh1236I	CGCG	2 cut(s)	213, 871
Bsh1285I	CGRYCG	2 cut(s)	217, 656
BshFI	GGCC	5 cut(s)	137, 216, 457, 958, 1069
BshNI	GGYRCC	1 cut(s)	6
BsiEI	CGRYCG	2 cut(s)	217, 656
BsiHKAI	GWGCWC	1 cut(s)	250
BsiSI	CCGG	6 cut(s)	256, 287, 595, 652, 665, 990
BslFI	GGGAC	2 cut(s)	685, 1358
BslI	CCNNNNNNNGG	7 cut(s)	467, 600, 601, 667, 668, 1065, 1361
BsmAI	GTCTC	2 cut(s)	284, 1049
BsmFI	GGGAC	2 cut(s)	685, 1358
BsmI	GAATGC	3 cut(s)	796, 1015, 1090
BsnI	GGCC	5 cut(s)	137, 216, 457, 958, 1069
Bso31I	GGTCTC	2 cut(s)	284, 1049
Bsp119I	TTCGAA	3 cut(s)	371, 380, 1100
Bsp1286I	GDGCHC	2 cut(s)	250, 533
Bsp1407I	TGTACA	1 cut(s)	1043
Bsp143I	GATC	7 cut(s)	105, 127, 315, 321, 577, 865, 1276
Bsp19I	CCATGG	1 cut(s)	184
BspANI	GGCC	5 cut(s)	137, 216, 457, 958, 1069
BspCNI	CTCAG	2 cut(s)	41, 612
BspFNI	CGCG	2 cut(s)	213, 871
BspHI	TCATGA	1 cut(s)	408
BspLI	GGNNCC	6 cut(s)	8, 650, 690, 891, 1008, 1245
BspMAI	CTGCAG	1 cut(s)	1495
BspMI	ACCTGC	1 cut(s)	1220
BspPI	GGATC	2 cut(s)	100, 860
BspT104I	TTCGAA	3 cut(s)	371, 380, 1100
BspT107I	GGYRCC	1 cut(s)	6
BspTNI	GGTCTC	2 cut(s)	284, 1049
BsrBI	CCGCTC	1 cut(s)	1455
BsrDI	GCAATG	2 cut(s)	918, 1446
BsrGI	TGTACA	1 cut(s)	1043
BsrI	ACTGG	1 cut(s)	1008
BssECI	CCNNGG	6 cut(s)	184, 211, 344, 501, 1059, 1137
BssMI	GATC	7 cut(s)	105, 127, 315, 321, 577, 865, 1276
BssNAI	GTATAC	1 cut(s)	1481
BssSI	CACGAG	1 cut(s)	274
BssT1I	CCWWGG	3 cut(s)	184, 501, 1137
Bst1107I	GTATAC	1 cut(s)	1481
Bst2BI	CACGAG	1 cut(s)	274
Bst2UI	CCWGG	1 cut(s)	1356
Bst4CI	ACNGT	4 cut(s)	125, 419, 859, 1060
Bst6I	CTCTTC	1 cut(s)	1116
BstAUI	TGTACA	1 cut(s)	1043
BstBI	TTCGAA	3 cut(s)	371, 380, 1100
BstC8I	GCNNGC	1 cut(s)	1488
BstDEI	CTNAG	5 cut(s)	28, 330, 620, 885, 1146
BstDSI	CCRYGG	3 cut(s)	184, 211, 1059
BstFNI	CGCG	2 cut(s)	213, 871
BstHHI	GCGC	1 cut(s)	286
BstKTI	GATC	7 cut(s)	108, 130, 318, 324, 580, 868, 1279
BstMAI	GTCTC	2 cut(s)	284, 1049
BstMBI	GATC	7 cut(s)	105, 127, 315, 321, 577, 865, 1276
BstMCI	CGRYCG	2 cut(s)	217, 656
BstMWI	GCNNNNNNNGC	8 cut(s)	29, 180, 186, 802, 838, 847, 920, 1142
BstNI	CCWGG	1 cut(s)	1356
BstNSI	RCATGY	1 cut(s)	1422
BstSCI	CCNGG	3 cut(s)	593, 989, 1354
BstSFI	CTRYAG	3 cut(s)	121, 783, 1491
BstUI	CGCG	2 cut(s)	213, 871
BstV1I	GCAGC	3 cut(s)	192, 935, 1477
BstX2I	RGATCY	2 cut(s)	105, 321
BstYI	RGATCY	2 cut(s)	105, 321
BstZ17I	GTATAC	1 cut(s)	1481
BstZI	CGGCCG	1 cut(s)	214
Bsu36I	CCTNAGG	1 cut(s)	885
BsuRI	GGCC	5 cut(s)	137, 216, 457, 958, 1069
BtgI	CCRYGG	3 cut(s)	184, 211, 1059
BtsIMutI	CAGTG	1 cut(s)	130
BveI	ACCTGC	1 cut(s)	1220
Cac8I	GCNNGC	1 cut(s)	1488
CaiI	CAGNNNCTG	1 cut(s)	1235
CciI	TCATGA	1 cut(s)	408
CfoI	GCGC	1 cut(s)	286
Cfr13I	GGNCC	2 cut(s)	688, 889
Cfr42I	CCGCGG	1 cut(s)	214
CviAII	CATG	5 cut(s)	185, 409, 1256, 1419, 1448
DdeI	CTNAG	5 cut(s)	28, 330, 620, 885, 1146
DpnI	GATC	7 cut(s)	107, 129, 317, 323, 579, 867, 1278
DpnII	GATC	7 cut(s)	105, 127, 315, 321, 577, 865, 1276
EaeI	YGGCCR	2 cut(s)	135, 214
EagI	CGGCCG	1 cut(s)	214
Eam1104I	CTCTTC	1 cut(s)	1116
EarI	CTCTTC	1 cut(s)	1116
EciI	GGCGGA	1 cut(s)	792
Ecl136II	GAGCTC	1 cut(s)	248
EclXI	CGGCCG	1 cut(s)	214
Eco130I	CCWWGG	3 cut(s)	184, 501, 1137
Eco24I	GRGCYC	2 cut(s)	250, 533
Eco31I	GGTCTC	2 cut(s)	284, 1049
Eco47I	GGWCC	2 cut(s)	688, 889
Eco52I	CGGCCG	1 cut(s)	214
Eco53kI	GAGCTC	1 cut(s)	248
Eco81I	CCTNAGG	1 cut(s)	885
EcoICRI	GAGCTC	1 cut(s)	248
EcoO109I	RGGNCCY	1 cut(s)	889
EcoRI	GAATTC	1 cut(s)	1171
EcoRII	CCWGG	1 cut(s)	1354
EcoT14I	CCWWGG	3 cut(s)	184, 501, 1137
EcoT38I	GRGCYC	2 cut(s)	250, 533
ErhI	CCWWGG	3 cut(s)	184, 501, 1137
FaeI	CATG	5 cut(s)	188, 412, 1259, 1422, 1451
FalI	AAGNNNNNCTT	2 cut(s)	375, 407
FaqI	GGGAC	2 cut(s)	685, 1358
FatI	CATG	5 cut(s)	184, 408, 1255, 1418, 1447
FauI	CCCGC	2 cut(s)	661, 837
FblI	GTMKAC	4 cut(s)	195, 306, 656, 1480
Fnu4HI	GCNGC	7 cut(s)	138, 181, 214, 924, 1067, 1456, 1491
FriOI	GRGCYC	2 cut(s)	250, 533
Fsp4HI	GCNGC	7 cut(s)	138, 181, 214, 924, 1067, 1456, 1491
FspBI	CTAG	3 cut(s)	221, 1155, 1308
GlaI	GCGC	1 cut(s)	285
GluI	GCNGC	7 cut(s)	138, 181, 214, 924, 1067, 1456, 1491
GsuI	CTGGAG	1 cut(s)	226
HaeIII	GGCC	5 cut(s)	137, 216, 457, 958, 1069
HapII	CCGG	6 cut(s)	256, 287, 595, 652, 665, 990
HhaI	GCGC	1 cut(s)	286
Hin1II	CATG	5 cut(s)	188, 412, 1259, 1422, 1451
Hin6I	GCGC	1 cut(s)	284
HinP1I	GCGC	1 cut(s)	284
HincII	GTYRAC	3 cut(s)	307, 657, 1152
HindII	GTYRAC	3 cut(s)	307, 657, 1152
HindIII	AAGCTT	1 cut(s)	164
HinfI	GANTC	7 cut(s)	26, 259, 334, 340, 377, 451, 1076
HpaII	CCGG	6 cut(s)	256, 287, 595, 652, 665, 990
Hpy166II	GTNNAC	8 cut(s)	196, 307, 657, 1001, 1044, 1152, 1416, 1481
Hpy188I	TCNGA	8 cut(s)	157, 266, 339, 345, 462, 621, 1192, 1215
Hpy188III	TCNNGA	5 cut(s)	103, 243, 319, 409, 1308
Hpy8I	GTNNAC	8 cut(s)	196, 307, 657, 1001, 1044, 1152, 1416, 1481
Hpy99I	CGWCG	2 cut(s)	44, 641
HpyAV	CCTTC	3 cut(s)	772, 816, 961
HpyCH4III	ACNGT	4 cut(s)	125, 419, 859, 1060
HpyF10VI	GCNNNNNNNGC	8 cut(s)	29, 180, 186, 802, 838, 847, 920, 1142
HpyF3I	CTNAG	5 cut(s)	28, 330, 620, 885, 1146
Hsp92II	CATG	5 cut(s)	188, 412, 1259, 1422, 1451
HspAI	GCGC	1 cut(s)	284
KpnI	GGTACC	1 cut(s)	10
KspI	CCGCGG	1 cut(s)	214
Kzo9I	GATC	7 cut(s)	105, 127, 315, 321, 577, 865, 1276
LmnI	GCTCC	5 cut(s)	213, 654, 949, 1219, 1243
Lsp1109I	GCAGC	3 cut(s)	192, 935, 1477
LweI	GCATC	3 cut(s)	1374, 1426, 1478
MaeI	CTAG	3 cut(s)	221, 1155, 1308
MaeIII	GTNAC	3 cut(s)	584, 700, 1358
MalI	GATC	7 cut(s)	107, 129, 317, 323, 579, 867, 1278
MbiI	CCGCTC	1 cut(s)	1455
MboI	GATC	7 cut(s)	105, 127, 315, 321, 577, 865, 1276
MboII	GAAGA	1 cut(s)	1133
MfeI	CAATTG	1 cut(s)	507
MflI	RGATCY	2 cut(s)	105, 321
MhlI	GDGCHC	2 cut(s)	250, 533
MlyI	GAGTC	3 cut(s)	20, 253, 349
MmeI	TCCRAC	3 cut(s)	133, 289, 738
MseI	TTAA	4 cut(s)	555, 767, 1032, 1341
MslI	CAYNNNNRTG	2 cut(s)	189, 1260
MspA1I	CMGCKG	2 cut(s)	89, 213
MspI	CCGG	6 cut(s)	256, 287, 595, 652, 665, 990
MspR9I	CCNGG	3 cut(s)	595, 991, 1356
MunI	CAATTG	1 cut(s)	507
Mva1269I	GAATGC	3 cut(s)	796, 1015, 1090
MvaI	CCWGG	1 cut(s)	1356
MvnI	CGCG	2 cut(s)	213, 871
MwoI	GCNNNNNNNGC	8 cut(s)	29, 180, 186, 802, 838, 847, 920, 1142
NciI	CCSGG	2 cut(s)	595, 991
NcoI	CCATGG	1 cut(s)	184
NdeII	GATC	7 cut(s)	105, 127, 315, 321, 577, 865, 1276
NlaIII	CATG	5 cut(s)	188, 412, 1259, 1422, 1451
NlaIV	GGNNCC	6 cut(s)	8, 650, 690, 891, 1008, 1245
NmeAIII	GCCGAG	1 cut(s)	44
NmuCI	GTSAC	1 cut(s)	700
NspI	RCATGY	1 cut(s)	1422
NspV	TTCGAA	3 cut(s)	371, 380, 1100
PagI	TCATGA	1 cut(s)	408
PciI	ACATGT	1 cut(s)	1418
PcsI	WCGNNNNNNNCGW	1 cut(s)	377
PctI	GAATGC	3 cut(s)	796, 1015, 1090
PfeI	GAWTC	4 cut(s)	334, 377, 451, 1076
PkrI	GCNGC	7 cut(s)	139, 182, 215, 925, 1068, 1457, 1492
PleI	GAGTC	3 cut(s)	20, 253, 348
PpsI	GAGTC	3 cut(s)	20, 253, 348
PpuMI	RGGWCCY	1 cut(s)	889
PscI	ACATGT	1 cut(s)	1418
PshBI	ATTAAT	1 cut(s)	1341
PsiI	TTATAA	1 cut(s)	836
Psp124BI	GAGCTC	1 cut(s)	250
Psp5II	RGGWCCY	1 cut(s)	889
Psp6I	CCWGG	1 cut(s)	1354
PspGI	CCWGG	1 cut(s)	1354
PspN4I	GGNNCC	6 cut(s)	8, 650, 690, 891, 1008, 1245
PspPI	GGNCC	2 cut(s)	688, 889
PspPPI	RGGWCCY	1 cut(s)	889
PsrI	GAACNNNNNNTAC	2 cut(s)	617, 649
PstI	CTGCAG	1 cut(s)	1495
PstNI	CAGNNNCTG	1 cut(s)	1235
PsuI	RGATCY	2 cut(s)	105, 321
RseI	CAYNNNNRTG	2 cut(s)	189, 1260
SacI	GAGCTC	1 cut(s)	250
SacII	CCGCGG	1 cut(s)	214
SalI	GTCGAC	2 cut(s)	305, 655
SaqAI	TTAA	4 cut(s)	555, 767, 1032, 1341
SatI	GCNGC	7 cut(s)	138, 181, 214, 924, 1067, 1456, 1491
Sau3AI	GATC	7 cut(s)	105, 127, 315, 321, 577, 865, 1276
Sau96I	GGNCC	2 cut(s)	688, 889
ScaI	AGTACT	1 cut(s)	721
SchI	GAGTC	3 cut(s)	20, 253, 349
ScrFI	CCNGG	3 cut(s)	595, 991, 1356
SduI	GDGCHC	2 cut(s)	250, 533
SfaNI	GCATC	3 cut(s)	1374, 1426, 1478
SfcI	CTRYAG	3 cut(s)	121, 783, 1491
Sfr303I	CCGCGG	1 cut(s)	214
SfuI	TTCGAA	3 cut(s)	371, 380, 1100
SgrBI	CCGCGG	1 cut(s)	214
SinI	GGWCC	2 cut(s)	688, 889
SmiMI	CAYNNNNRTG	2 cut(s)	189, 1260
SspMI	CTAG	3 cut(s)	221, 1155, 1308
SstI	GAGCTC	1 cut(s)	250
StyD4I	CCNGG	3 cut(s)	593, 989, 1354
StyI	CCWWGG	3 cut(s)	184, 501, 1137
TaaI	ACNGT	4 cut(s)	125, 419, 859, 1060
TatI	WGTACW	2 cut(s)	719, 1043
TauI	GCSGC	4 cut(s)	140, 216, 1069, 1458
TfiI	GAWTC	4 cut(s)	334, 377, 451, 1076
Tru1I	TTAA	4 cut(s)	555, 767, 1032, 1341
Tru9I	TTAA	4 cut(s)	555, 767, 1032, 1341
TscAI	CASTG	1 cut(s)	130
TseFI	GTSAC	1 cut(s)	700
TseI	GCWGC	3 cut(s)	180, 923, 1490
Tsp45I	GTSAC	1 cut(s)	700
TspDTI	ATGAA	5 cut(s)	397, 513, 533, 980, 1244
TspGWI	ACGGA	2 cut(s)	28, 1029
TspRI	CASTG	1 cut(s)	130
VpaK11BI	GGWCC	2 cut(s)	688, 889
VspI	ATTAAT	1 cut(s)	1341
XapI	RAATTY	3 cut(s)	1126, 1171, 1497
XbaI	TCTAGA	1 cut(s)	1307
XceI	RCATGY	1 cut(s)	1422
XmiI	GTMKAC	4 cut(s)	195, 306, 656, 1480
XspI	CTAG	3 cut(s)	221, 1155, 1308
ZrmI	AGTACT	1 cut(s)	721

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.