Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr07

Orthogroup ID

OG0012915

Physical Location & Seq

Genomic Coordinates Reverse (-)

2098716 .. 2100212

Gene Structure 1497 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD07G1025000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1497 bp

ATGGGGTACCTTTCCTGCAGAGCTCAATCAGCCATCTCCGTCGCCAATTCCCAAACCTCATCGTCCAAAACCACCGCCACCGCCACCCCGGAAAACCCCATCAAGATCCAACACTTCGACTACAGTGATCTTGTCGCCGCCACCAATGGCTTCTCCGACCAGAAGCTTTTAGGCAAAGGCAGCCATGGCTACGTTTACAAAGCTGTGCTCCGTGGCCGTCTTGTTGCCGTCAAAAGACCCTCGAGAGCTCACAGCCAGAGACTCCGACCCACCTCGTCTTCTGGGCCGGAGACCGCGAACGAGGTCGACAATGAGCTCGAGATCTTGTCCAAGATTCAGAGTCCGAGGCTGGTCAATCTTTTGGGGTTTTCGAATGATTCGAAAGAAAGGCTTTTGGTGGTGGAGTTCATGAGCAACGGTACGCTGTACGATGTTTTGCATTCGAGTACAAATCGGCCTCCGAATTGGGGTCGTAGGATTAGATTAGCTTTGCAAACATCTAAGGCAATTGACATTTTACATTCATCGAACCCACCTGTAATTCATAGAGATATTAAGTCTGCTAATGTTTTGATTGATCGTAGTTACAATGCCCGGTTGGGGGATTTTGGATTGGCTCTGAGGTACTGCCAGTTCGACGAGTATAGGCTCCGGTCCACCCCGCCGGCGGGGACGATGGGGTACTTGGACCCTTGCTATGTGACCCCCGACAATTTGAGTACTAAAACTGATGTTTTTAGTTTTGGGATTTTGTTGTTGGAGATTATAAGTGGTAGGAAGGCTATTGATGTTGGGCATTCTCCGCCTTCGATCGTTGATTGGGCTATCCCGCTTATAAAGCGAGGGAAGCTGATAACGGTTTATGATCCGCGAATTGTACCTCCTAAAGACCCTGTTGTGAGGAAGCAATTAGCTGTGATTGCAGCCAAGTGTGTGAGGTCTTGTAGGGAGCGTAGGCCATCGATGAAGGAGGTCGTGGTTTGGCTTACTGGGTTGAGTAAACTGGTTCCCCTGCATTCGTGGAATGGTTTTAACAATCCATGTACAATGGTAGAGACCATGGGGCGCCCGGTTGAATCAAGGAATGCACATTTGACTTCGAAGCTAGAGGGTGTTGGAGAAGAGAATTTGGAAGCCCTAGAAGCGAAATTGACTAGGCAAGCGTTGAGGAATTCGCGGCGAGTTTATTCAGATTTGGGGTTTAGCACCAATCTGATGGAGCTTATGGCAGGTACTGATGGGGAGTCGGAGTTCCATGGTAACGCAGATGGAGATAACTCCAGTTCAAAATCGGCGAATCTGGTTTCTAGATTTGGTAGTGCAAGATATATTGGTAGGAGTAATCAATCAACTCCTGGTAATGGGAAAGGAGTCCCTGATTTGATGCAAAATCATTCTTTTGGGAAAAAGTCATTTACATGTTCAAGGAGAGATGATGCAATGCCTCATGTGAGCGGCAATTCTCGTGCAGCTGCTGGTATACAGCTTGCTGCATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004675 GO:0004888 GO:0005575 GO:0005623 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0007154 GO:0007165 GO:0007166 GO:0007167 GO:0007178 GO:0008150 GO:0008152 GO:0009987 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019199 GO:0019538 GO:0023052 GO:0036211 GO:0038023 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044464 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0060089 GO:0065007 GO:0071704 GO:0071944 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

ko04010 ko04064 ko04620 ko04621 ko04624 ko04722 ko05133 ko05140 ko05142 ko05145 ko05152 ko05162 ko05164 ko05169 map04010 map04064 map04620 map04621 map04624 map04722 map05133 map05140 map05142 map05145 map05152 map05162 map05164 map05169

Pfam Domains

Protein Families

Pkinase Pkinase_Tyr

Protein Analysis

499

Amino Acids

54.61

Weight (kDa)

9.62

Isoelectric Point (pI)

38.5

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Pkinase	PF00069	52 - 326	1.6e-36	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	54 - 326	8.5e-35	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0012915)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G51990
fragaria_vesca	FvH4_7g01890
malus_domestica	MD02G1299900.v1.1 MD07G1025000.v1.1
prunus_persica	Prupe.2G024700_v2.0.a1
pyrus_communis	pycom02g25190 pycom03g05450 pycom07g01930
rosa_chinensis	RchiOBHm_Chr1g0318491
rosa_laevigata	RLG00000030533
rosa_multiflora	Rmu_co8489629.1_g000001
rosa_roxburghii	Rroxscaffold_4G00329110
rosa_rugosa	Rorug01G0021700
rosa_samantha	Rh1AG034100 Rh1BG030200 Rh1CG033600 Rh1DG037000
rosa_wichuraiana	Rw1G002710

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	767, 836
Acc36I	ACCTGC	1 cut(s)	1220
Acc65I	GGTACC	1 cut(s)	6
AccB1I	GGYRCC	2 cut(s)	6, 1065
AccBSI	CCGCTC	1 cut(s)	1455
AccI	GTMKAC	2 cut(s)	306, 1480
AccII	CGCG	3 cut(s)	296, 871, 1177
AclWI	GGATC	2 cut(s)	100, 860
AcoI	YGGCCR	1 cut(s)	214
AcsI	RAATTY	2 cut(s)	1126, 1171
AcyI	GRCGYC	1 cut(s)	1066
AfiI	CCNNNNNNNGG	6 cut(s)	467, 600, 601, 667, 668, 1361
AflIII	ACRYGT	1 cut(s)	1418
AgsI	TTSAA	3 cut(s)	1076, 1287, 1425
AjnI	CCWGG	1 cut(s)	1354
AloI	GAACNNNNNNTCC	2 cut(s)	1235, 1267
Alw21I	GWGCWC	4 cut(s)	25, 210, 250, 318
Alw26I	GTCTC	3 cut(s)	253, 284, 1049
AlwI	GGATC	2 cut(s)	100, 860
AlwNI	CAGNNNCTG	2 cut(s)	1235, 1475
Ama87I	CYCGRG	2 cut(s)	241, 317
AoxI	GGCC	4 cut(s)	214, 284, 455, 956
ApeKI	GCWGC	5 cut(s)	180, 923, 1469, 1472, 1490
ApoI	RAATTY	2 cut(s)	1126, 1171
Asp718I	GGTACC	1 cut(s)	6
AspLEI	GCGC	1 cut(s)	1068
AspS9I	GGNCC	3 cut(s)	284, 654, 688
AsuC2I	CCSGG	3 cut(s)	89, 595, 1070
AsuII	TTCGAA	3 cut(s)	371, 380, 1100
AvaI	CYCGRG	2 cut(s)	241, 317
AvaII	GGWCC	2 cut(s)	654, 688
BanI	GGYRCC	2 cut(s)	6, 1065
BanII	GRGCYC	3 cut(s)	25, 250, 318
BauI	CACGAG	1 cut(s)	1464
BbsI	GAAGAC	1 cut(s)	270
Bbv12I	GWGCWC	4 cut(s)	25, 210, 250, 318
BbvI	GCAGC	5 cut(s)	192, 935, 1459, 1477, 1481
BccI	CCATC	7 cut(s)	41, 107, 670, 967, 1210, 1232, 1262
BceAI	ACGGC	2 cut(s)	201, 212
BciT130I	CCWGG	1 cut(s)	1356
BcnI	CCSGG	3 cut(s)	89, 595, 1070
BcoDI	GTCTC	3 cut(s)	253, 284, 1049
BfaI	CTAG	4 cut(s)	1106, 1139, 1155, 1308
BfmI	CTRYAG	2 cut(s)	16, 121
BfoI	RGCGCY	1 cut(s)	1069
BfuAI	ACCTGC	1 cut(s)	1220
BglII	AGATCT	1 cut(s)	321
BisI	GCNGC	8 cut(s)	138, 181, 924, 1178, 1456, 1470, 1473, 1491
BlsI	GCNGC	8 cut(s)	139, 182, 925, 1179, 1457, 1471, 1474, 1492
BmcAI	AGTACT	1 cut(s)	721
Bme1390I	CCNGG	4 cut(s)	89, 595, 1070, 1356
Bme18I	GGWCC	2 cut(s)	654, 688
BmeT110I	CYCGRG	2 cut(s)	241, 317
BmgT120I	GGNCC	3 cut(s)	284, 654, 688
BmiI	GGNNCC	5 cut(s)	8, 650, 690, 1008, 1067
BmrFI	CCNGG	4 cut(s)	89, 595, 1070, 1356
BmrI	ACTGGG	1 cut(s)	999
BmsI	GCATC	2 cut(s)	1374, 1426
BmuI	ACTGGG	1 cut(s)	999
BpiI	GAAGAC	1 cut(s)	270
BpmI	CTGGAG	1 cut(s)	1264
Bpu14I	TTCGAA	3 cut(s)	371, 380, 1100
BpuMI	CCSGG	3 cut(s)	89, 595, 1070
Bsa29I	ATCGAT	1 cut(s)	962
BsaHI	GRCGYC	1 cut(s)	1066
BsaI	GGTCTC	2 cut(s)	284, 1049
BsaJI	CCNNGG	6 cut(s)	87, 184, 211, 344, 1059, 1255
BsaWI	WCCGGW	1 cut(s)	651
BsaXI	ACNNNNNCTCC	2 cut(s)	1235, 1265
Bsc4I	CCNNNNNNNGG	6 cut(s)	467, 600, 601, 667, 668, 1361
Bse118I	RCCGGY	1 cut(s)	664
Bse1I	ACTGG	4 cut(s)	631, 994, 1008, 1281
Bse3DI	GCAATG	1 cut(s)	1446
BseBI	CCWGG	1 cut(s)	1356
BseCI	ATCGAT	1 cut(s)	962
BseDI	CCNNGG	6 cut(s)	87, 184, 211, 344, 1059, 1255
BseLI	CCNNNNNNNGG	6 cut(s)	467, 600, 601, 667, 668, 1361
BseMI	GCAATG	1 cut(s)	1446
BseMII	CTCAG	1 cut(s)	611
BseNI	ACTGG	4 cut(s)	631, 994, 1008, 1281
BseXI	GCAGC	5 cut(s)	192, 935, 1459, 1477, 1481
BsgI	GTGCAG	1 cut(s)	1488
Bsh1236I	CGCG	3 cut(s)	296, 871, 1177
Bsh1285I	CGRYCG	1 cut(s)	813
BshFI	GGCC	4 cut(s)	216, 286, 457, 958
BshNI	GGYRCC	2 cut(s)	6, 1065
BshVI	ATCGAT	1 cut(s)	962
BsiEI	CGRYCG	1 cut(s)	813
BsiHKAI	GWGCWC	4 cut(s)	25, 210, 250, 318
BsiHKCI	CYCGRG	2 cut(s)	241, 317
BsiSI	CCGG	6 cut(s)	89, 287, 595, 652, 665, 1070
BslFI	GGGAC	2 cut(s)	685, 1358
BslI	CCNNNNNNNGG	6 cut(s)	467, 600, 601, 667, 668, 1361
BsmAI	GTCTC	3 cut(s)	253, 284, 1049
BsmFI	GGGAC	2 cut(s)	685, 1358
BsmI	GAATGC	4 cut(s)	439, 796, 1015, 1090
BsnI	GGCC	4 cut(s)	216, 286, 457, 958
Bso31I	GGTCTC	2 cut(s)	284, 1049
BsoBI	CYCGRG	2 cut(s)	241, 317
Bsp119I	TTCGAA	3 cut(s)	371, 380, 1100
Bsp1286I	GDGCHC	4 cut(s)	25, 210, 250, 318
Bsp1407I	TGTACA	1 cut(s)	1043
Bsp143I	GATC	6 cut(s)	105, 127, 321, 577, 810, 865
Bsp19I	CCATGG	3 cut(s)	184, 1059, 1255
BspANI	GGCC	4 cut(s)	216, 286, 457, 958
BspCNI	CTCAG	1 cut(s)	612
BspDI	ATCGAT	1 cut(s)	962
BspFNI	CGCG	3 cut(s)	296, 871, 1177
BspHI	TCATGA	1 cut(s)	408
BspLI	GGNNCC	5 cut(s)	8, 650, 690, 1008, 1067
BspMAI	CTGCAG	1 cut(s)	20
BspMI	ACCTGC	1 cut(s)	1220
BspPI	GGATC	2 cut(s)	100, 860
BspT104I	TTCGAA	3 cut(s)	371, 380, 1100
BspT107I	GGYRCC	2 cut(s)	6, 1065
BspTNI	GGTCTC	2 cut(s)	284, 1049
BsrBI	CCGCTC	1 cut(s)	1455
BsrDI	GCAATG	1 cut(s)	1446
BsrFI	RCCGGY	1 cut(s)	664
BsrGI	TGTACA	1 cut(s)	1043
BsrI	ACTGG	4 cut(s)	631, 994, 1008, 1281
BssAI	RCCGGY	1 cut(s)	664
BssECI	CCNNGG	6 cut(s)	87, 184, 211, 344, 1059, 1255
BssMI	GATC	6 cut(s)	105, 127, 321, 577, 810, 865
BssNAI	GTATAC	1 cut(s)	1481
BssNI	GRCGYC	1 cut(s)	1066
BssSI	CACGAG	1 cut(s)	1464
BssT1I	CCWWGG	3 cut(s)	184, 1059, 1255
Bst1107I	GTATAC	1 cut(s)	1481
Bst2BI	CACGAG	1 cut(s)	1464
Bst2UI	CCWGG	1 cut(s)	1356
Bst4CI	ACNGT	3 cut(s)	125, 419, 859
Bst6I	CTCTTC	1 cut(s)	1116
BstACI	GRCGYC	1 cut(s)	1066
BstAUI	TGTACA	1 cut(s)	1043
BstBI	TTCGAA	3 cut(s)	371, 380, 1100
BstC8I	GCNNGC	3 cut(s)	666, 1161, 1488
BstDEI	CTNAG	2 cut(s)	501, 620
BstDSI	CCRYGG	4 cut(s)	184, 211, 1059, 1255
BstFNI	CGCG	3 cut(s)	296, 871, 1177
BstH2I	RGCGCY	1 cut(s)	1069
BstHHI	GCGC	1 cut(s)	1068
BstKTI	GATC	6 cut(s)	108, 130, 324, 580, 813, 868
BstMAI	GTCTC	3 cut(s)	253, 284, 1049
BstMBI	GATC	6 cut(s)	105, 127, 321, 577, 810, 865
BstMCI	CGRYCG	1 cut(s)	813
BstMWI	GCNNNNNNNGC	8 cut(s)	29, 180, 186, 802, 838, 847, 920, 1142
BstNI	CCWGG	1 cut(s)	1356
BstNSI	RCATGY	1 cut(s)	1422
BstSCI	CCNGG	4 cut(s)	87, 593, 1068, 1354
BstSFI	CTRYAG	2 cut(s)	16, 121
BstUI	CGCG	3 cut(s)	296, 871, 1177
BstV1I	GCAGC	5 cut(s)	192, 935, 1459, 1477, 1481
BstV2I	GAAGAC	1 cut(s)	270
BstX2I	RGATCY	2 cut(s)	105, 321
BstXI	CCANNNNNNTGG	1 cut(s)	1216
BstYI	RGATCY	2 cut(s)	105, 321
BstZ17I	GTATAC	1 cut(s)	1481
Bsu15I	ATCGAT	1 cut(s)	962
BsuRI	GGCC	4 cut(s)	216, 286, 457, 958
BsuTUI	ATCGAT	1 cut(s)	962
BtgI	CCRYGG	4 cut(s)	184, 211, 1059, 1255
BtsIMutI	CAGTG	1 cut(s)	130
BveI	ACCTGC	1 cut(s)	1220
Cac8I	GCNNGC	3 cut(s)	666, 1161, 1488
CaiI	CAGNNNCTG	2 cut(s)	1235, 1475
CciI	TCATGA	1 cut(s)	408
CfoI	GCGC	1 cut(s)	1068
Cfr10I	RCCGGY	1 cut(s)	664
Cfr13I	GGNCC	3 cut(s)	284, 654, 688
ClaI	ATCGAT	1 cut(s)	962
CviAII	CATG	7 cut(s)	185, 409, 1041, 1060, 1256, 1419, 1448
DdeI	CTNAG	2 cut(s)	501, 620
DinI	GGCGCC	1 cut(s)	1067
DpnI	GATC	6 cut(s)	107, 129, 323, 579, 812, 867
DpnII	GATC	6 cut(s)	105, 127, 321, 577, 810, 865
EaeI	YGGCCR	1 cut(s)	214
Eam1104I	CTCTTC	1 cut(s)	1116
EarI	CTCTTC	1 cut(s)	1116
EciI	GGCGGA	1 cut(s)	792
Ecl136II	GAGCTC	3 cut(s)	23, 248, 316
Eco130I	CCWWGG	3 cut(s)	184, 1059, 1255
Eco24I	GRGCYC	3 cut(s)	25, 250, 318
Eco31I	GGTCTC	2 cut(s)	284, 1049
Eco47I	GGWCC	2 cut(s)	654, 688
Eco53kI	GAGCTC	3 cut(s)	23, 248, 316
Eco88I	CYCGRG	2 cut(s)	241, 317
EcoICRI	GAGCTC	3 cut(s)	23, 248, 316
EcoRI	GAATTC	1 cut(s)	1171
EcoRII	CCWGG	1 cut(s)	1354
EcoT14I	CCWWGG	3 cut(s)	184, 1059, 1255
EcoT38I	GRGCYC	3 cut(s)	25, 250, 318
EgeI	GGCGCC	1 cut(s)	1067
EheI	GGCGCC	1 cut(s)	1067
ErhI	CCWWGG	3 cut(s)	184, 1059, 1255
FaeI	CATG	7 cut(s)	188, 412, 1044, 1063, 1259, 1422, 1451
FalI	AAGNNNNNCTT	2 cut(s)	375, 407
FaqI	GGGAC	2 cut(s)	685, 1358
FatI	CATG	7 cut(s)	184, 408, 1040, 1059, 1255, 1418, 1447
FauI	CCCGC	3 cut(s)	661, 669, 837
FblI	GTMKAC	2 cut(s)	306, 1480
Fnu4HI	GCNGC	8 cut(s)	138, 181, 924, 1178, 1456, 1470, 1473, 1491
FriOI	GRGCYC	3 cut(s)	25, 250, 318
Fsp4HI	GCNGC	8 cut(s)	138, 181, 924, 1178, 1456, 1470, 1473, 1491
FspBI	CTAG	4 cut(s)	1106, 1139, 1155, 1308
GlaI	GCGC	1 cut(s)	1067
GluI	GCNGC	8 cut(s)	138, 181, 924, 1178, 1456, 1470, 1473, 1491
GsuI	CTGGAG	1 cut(s)	1264
HaeII	RGCGCY	1 cut(s)	1069
HaeIII	GGCC	4 cut(s)	216, 286, 457, 958
HapII	CCGG	6 cut(s)	89, 287, 595, 652, 665, 1070
HhaI	GCGC	1 cut(s)	1068
Hin1I	GRCGYC	1 cut(s)	1066
Hin1II	CATG	7 cut(s)	188, 412, 1044, 1063, 1259, 1422, 1451
Hin6I	GCGC	1 cut(s)	1066
HinP1I	GCGC	1 cut(s)	1066
HincII	GTYRAC	1 cut(s)	307
HindII	GTYRAC	1 cut(s)	307
HindIII	AAGCTT	1 cut(s)	164
HinfI	GANTC	8 cut(s)	261, 334, 340, 377, 1076, 1244, 1297, 1371
HpaII	CCGG	6 cut(s)	89, 287, 595, 652, 665, 1070
Hpy166II	GTNNAC	5 cut(s)	196, 307, 657, 1001, 1481
Hpy188I	TCNGA	9 cut(s)	157, 266, 339, 345, 462, 621, 1192, 1215, 1249
Hpy188III	TCNNGA	5 cut(s)	103, 243, 319, 409, 1308
Hpy8I	GTNNAC	5 cut(s)	196, 307, 657, 1001, 1481
Hpy99I	CGWCG	2 cut(s)	44, 641
HpyAV	CCTTC	3 cut(s)	772, 816, 961
HpyCH4III	ACNGT	3 cut(s)	125, 419, 859
HpyCH4IV	ACGT	1 cut(s)	192
HpyF10VI	GCNNNNNNNGC	8 cut(s)	29, 180, 186, 802, 838, 847, 920, 1142
HpyF3I	CTNAG	2 cut(s)	501, 620
HpySE526I	ACGT	1 cut(s)	192
Hsp92I	GRCGYC	1 cut(s)	1066
Hsp92II	CATG	7 cut(s)	188, 412, 1044, 1063, 1259, 1422, 1451
HspAI	GCGC	1 cut(s)	1066
KasI	GGCGCC	1 cut(s)	1065
KpnI	GGTACC	1 cut(s)	10
KroI	GCCGGC	1 cut(s)	664
KroNI	GCCGGC	1 cut(s)	666
Kzo9I	GATC	6 cut(s)	105, 127, 321, 577, 810, 865
LmnI	GCTCC	4 cut(s)	213, 654, 949, 1219
Lsp1109I	GCAGC	5 cut(s)	192, 935, 1459, 1477, 1481
LweI	GCATC	2 cut(s)	1374, 1426
MaeI	CTAG	4 cut(s)	1106, 1139, 1155, 1308
MaeII	ACGT	1 cut(s)	192
MaeIII	GTNAC	3 cut(s)	584, 700, 1259
MalI	GATC	6 cut(s)	107, 129, 323, 579, 812, 867
MbiI	CCGCTC	1 cut(s)	1455
MboI	GATC	6 cut(s)	105, 127, 321, 577, 810, 865
MboII	GAAGA	2 cut(s)	270, 1133
MfeI	CAATTG	1 cut(s)	507
MflI	RGATCY	2 cut(s)	105, 321
MhlI	GDGCHC	4 cut(s)	25, 210, 250, 318
Mly113I	GGCGCC	1 cut(s)	1066
MlyI	GAGTC	4 cut(s)	255, 349, 1253, 1380
MmeI	TCCRAC	6 cut(s)	133, 180, 289, 738, 1096, 1227
MreI	CGCCGGCG	1 cut(s)	664
MroNI	GCCGGC	1 cut(s)	664
MseI	TTAA	2 cut(s)	555, 1032
MslI	CAYNNNNRTG	1 cut(s)	1417
MspA1I	CMGCKG	1 cut(s)	1472
MspI	CCGG	6 cut(s)	89, 287, 595, 652, 665, 1070
MspR9I	CCNGG	4 cut(s)	89, 595, 1070, 1356
MunI	CAATTG	1 cut(s)	507
Mva1269I	GAATGC	4 cut(s)	439, 796, 1015, 1090
MvaI	CCWGG	1 cut(s)	1356
MvnI	CGCG	3 cut(s)	296, 871, 1177
MwoI	GCNNNNNNNGC	8 cut(s)	29, 180, 186, 802, 838, 847, 920, 1142
NaeI	GCCGGC	1 cut(s)	666
NarI	GGCGCC	1 cut(s)	1066
NciI	CCSGG	3 cut(s)	89, 595, 1070
NcoI	CCATGG	3 cut(s)	184, 1059, 1255
NdeII	GATC	6 cut(s)	105, 127, 321, 577, 810, 865
NgoMIV	GCCGGC	1 cut(s)	664
NlaIII	CATG	7 cut(s)	188, 412, 1044, 1063, 1259, 1422, 1451
NlaIV	GGNNCC	5 cut(s)	8, 650, 690, 1008, 1067
NmuCI	GTSAC	1 cut(s)	700
NspI	RCATGY	1 cut(s)	1422
NspV	TTCGAA	3 cut(s)	371, 380, 1100
PaeR7I	CTCGAG	2 cut(s)	241, 317
PagI	TCATGA	1 cut(s)	408
PciI	ACATGT	1 cut(s)	1418
PcsI	WCGNNNNNNNCGW	1 cut(s)	377
PctI	GAATGC	4 cut(s)	439, 796, 1015, 1090
PdiI	GCCGGC	1 cut(s)	666
PfeI	GAWTC	4 cut(s)	334, 377, 1076, 1297
PkrI	GCNGC	8 cut(s)	139, 182, 925, 1179, 1457, 1471, 1474, 1492
Ple19I	CGATCG	1 cut(s)	813
PleI	GAGTC	4 cut(s)	255, 348, 1252, 1379
PluTI	GGCGCC	1 cut(s)	1069
PpsI	GAGTC	4 cut(s)	255, 348, 1252, 1379
PscI	ACATGT	1 cut(s)	1418
PsiI	TTATAA	2 cut(s)	767, 836
Psp124BI	GAGCTC	3 cut(s)	25, 250, 318
Psp6I	CCWGG	1 cut(s)	1354
PspGI	CCWGG	1 cut(s)	1354
PspN4I	GGNNCC	5 cut(s)	8, 650, 690, 1008, 1067
PspPI	GGNCC	3 cut(s)	284, 654, 688
PsrI	GAACNNNNNNTAC	2 cut(s)	617, 649
PstI	CTGCAG	1 cut(s)	20
PstNI	CAGNNNCTG	2 cut(s)	1235, 1475
PsuI	RGATCY	2 cut(s)	105, 321
PvuI	CGATCG	1 cut(s)	813
PvuII	CAGCTG	1 cut(s)	1472
RseI	CAYNNNNRTG	1 cut(s)	1417
SacI	GAGCTC	3 cut(s)	25, 250, 318
SalI	GTCGAC	1 cut(s)	305
SaqAI	TTAA	2 cut(s)	555, 1032
SatI	GCNGC	8 cut(s)	138, 181, 924, 1178, 1456, 1470, 1473, 1491
Sau3AI	GATC	6 cut(s)	105, 127, 321, 577, 810, 865
Sau96I	GGNCC	3 cut(s)	284, 654, 688
ScaI	AGTACT	1 cut(s)	721
SchI	GAGTC	4 cut(s)	255, 349, 1253, 1380
ScrFI	CCNGG	4 cut(s)	89, 595, 1070, 1356
SduI	GDGCHC	4 cut(s)	25, 210, 250, 318
SfaNI	GCATC	2 cut(s)	1374, 1426
SfcI	CTRYAG	2 cut(s)	16, 121
SfoI	GGCGCC	1 cut(s)	1067
Sfr274I	CTCGAG	2 cut(s)	241, 317
SfuI	TTCGAA	3 cut(s)	371, 380, 1100
SgrAI	CRCCGGYG	1 cut(s)	664
SinI	GGWCC	2 cut(s)	654, 688
SlaI	CTCGAG	2 cut(s)	241, 317
SmiMI	CAYNNNNRTG	1 cut(s)	1417
SmlI	CTYRAG	2 cut(s)	241, 317
SmoI	CTYRAG	2 cut(s)	241, 317
SspDI	GGCGCC	1 cut(s)	1065
SspMI	CTAG	4 cut(s)	1106, 1139, 1155, 1308
SstI	GAGCTC	3 cut(s)	25, 250, 318
StyD4I	CCNGG	4 cut(s)	87, 593, 1068, 1354
StyI	CCWWGG	3 cut(s)	184, 1059, 1255
TaaI	ACNGT	3 cut(s)	125, 419, 859
TaiI	ACGT	1 cut(s)	195
TatI	WGTACW	3 cut(s)	446, 719, 1043
TauI	GCSGC	3 cut(s)	140, 1180, 1458
TfiI	GAWTC	4 cut(s)	334, 377, 1076, 1297
Tru1I	TTAA	2 cut(s)	555, 1032
Tru9I	TTAA	2 cut(s)	555, 1032
TscAI	CASTG	1 cut(s)	130
TseFI	GTSAC	1 cut(s)	700
TseI	GCWGC	5 cut(s)	180, 923, 1469, 1472, 1490
Tsp45I	GTSAC	1 cut(s)	700
TspDTI	ATGAA	4 cut(s)	397, 513, 533, 980
TspGWI	ACGGA	2 cut(s)	28, 200
TspRI	CASTG	1 cut(s)	130
VpaK11BI	GGWCC	2 cut(s)	654, 688
XapI	RAATTY	2 cut(s)	1126, 1171
XbaI	TCTAGA	1 cut(s)	1307
XceI	RCATGY	1 cut(s)	1422
XhoI	CTCGAG	2 cut(s)	241, 317
XmiI	GTMKAC	2 cut(s)	306, 1480
XspI	CTAG	4 cut(s)	1106, 1139, 1155, 1308
ZrmI	AGTACT	1 cut(s)	721

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.