MD02G1302700.v1.1

serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0009722
Physical Location & Seq
Forward (+)
35678230 .. 35679632
1403 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1302700.v1.1.491

Sequence Viewer

Length: 561 bp
ATGCTATCAAATTTAAGTTCATTGCATGTCCTGGACCTTGCAGAAAATCAACTGAATGGAAGCATTCCAGCTAGTTTTGGAGATTTCAAAGCAATGGCGCAGGTGGAAAACACGAACCGTTATCTATTCTATGGGAAGTACAGGGGTGATTACTATGAAGAAAGCTTGGTTGTGAACTTAAAAGGCAAGCCTCAAATTCACACCAAGTTTCTCTTCCTCGTAATCAGCATAGACGTATCTGGAAATCAATTGAGTGGAGATATTCCCGAAGAAATAACTAATTTGGCTGGTTTGATTGCTCTGGATTTATCCAGAAACCACATAAGCGGTCACATTCCTGAAGGCATTTCAAAGTTGAAACAGTTAGAGTCACTTGATCTGTCAAGCAACAAGCTCACAGGTCCAATTCCTCGAAGTTTGTCTTTACTATCATTCCTGGGGTATCTGAATCTGTCAAACAACGACTTCACCGGTAGGATCCCATACACAGGGCAATTAACAACTTTCGATGCGCCATTTTTGCTGGAAATCCAGGCCTCTGTGGTGCTCCTTTTCTTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003002 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004675 GO:0004888 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005768 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006810 GO:0006897 GO:0006898 GO:0006950 GO:0006952 GO:0007154 GO:0007165 GO:0007166 GO:0007167 GO:0007178 GO:0007275 GO:0007389 GO:0008150 GO:0008152 GO:0009595 GO:0009605 GO:0009607 GO:0009617 GO:0009719 GO:0009725 GO:0009733 GO:0009734 GO:0009741 GO:0009742 GO:0009755 GO:0009987 GO:0010033 GO:0010051 GO:0010232 GO:0010233 GO:0010305 GO:0010359 GO:0012505 GO:0014070 GO:0016020 GO:0016043 GO:0016045 GO:0016192 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019199 GO:0019538 GO:0022898 GO:0023052 GO:0031410 GO:0031982 GO:0032409 GO:0032412 GO:0032501 GO:0032502 GO:0032870 GO:0032879 GO:0033036 GO:0033037 GO:0033554 GO:0033993 GO:0034762 GO:0034765 GO:0036211 GO:0038023 GO:0042221 GO:0042545 GO:0042742 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043269 GO:0043401 GO:0043412 GO:0044070 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0045229 GO:0048545 GO:0048856 GO:0050789 GO:0050794 GO:0050896 GO:0051049 GO:0051179 GO:0051234 GO:0051606 GO:0051641 GO:0051704 GO:0051707 GO:0051716 GO:0052386 GO:0052482 GO:0052542 GO:0052543 GO:0052544 GO:0052545 GO:0060089 GO:0065007 GO:0065009 GO:0070727 GO:0070887 GO:0071310 GO:0071365 GO:0071367 GO:0071383 GO:0071396 GO:0071407 GO:0071495 GO:0071554 GO:0071555 GO:0071704 GO:0071840 GO:0071944 GO:0097708 GO:0098542 GO:0098543 GO:0098581 GO:0098657 GO:0140096 GO:1901564 GO:1901700 GO:1901701 GO:1903959
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LCM LRRNT_2 LRR_1 LRR_4 LRR_6 LRR_8

Protein Analysis

187

Amino Acids

20.52

Weight (kDa)

5.28

Isoelectric Point (pI)

25.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_14 PF23598 86 - 152 8.2e-06 Leucine-rich repeat region
LRR_8 PF13855 117 - 156 6.1e-06 Leucine rich repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 91
Acc36I ACCTGC 1 cut(s) 91
AciI CCGC 1 cut(s) 327
AclWI GGATC 2 cut(s) 472, 485
AcsI RAATTY 2 cut(s) 10, 195
AcuI CTGAAG 1 cut(s) 360
AfaI GTAC 1 cut(s) 140
AfiI CCNNNNNNNGG 1 cut(s) 488
AgeI ACCGGT 1 cut(s) 470
AgsI TTSAA 3 cut(s) 88, 351, 358
AjnI CCWGG 3 cut(s) 30, 435, 531
AjuI GAANNNNNNNTTGG 2 cut(s) 197, 229
AluBI AGCT 3 cut(s) 71, 165, 394
AluI AGCT 3 cut(s) 71, 165, 394
Alw21I GWGCWC 1 cut(s) 549
AlwI GGATC 2 cut(s) 472, 485
AoxI GGCC 1 cut(s) 534
ApoI RAATTY 2 cut(s) 10, 195
AsiGI ACCGGT 1 cut(s) 470
AspLEI GCGC 2 cut(s) 100, 514
AspS9I GGNCC 2 cut(s) 34, 401
AsuHPI GGTGA 2 cut(s) 158, 460
AvaII GGWCC 2 cut(s) 34, 401
BamHI GGATCC 1 cut(s) 477
Bbv12I GWGCWC 1 cut(s) 549
BciT130I CCWGG 3 cut(s) 32, 437, 533
BfaI CTAG 1 cut(s) 72
BfuAI ACCTGC 1 cut(s) 91
Bme1390I CCNGG 3 cut(s) 32, 437, 533
Bme18I GGWCC 2 cut(s) 34, 401
BmgT120I GGNCC 2 cut(s) 34, 401
BmiI GGNNCC 1 cut(s) 479
BmrFI CCNGG 3 cut(s) 32, 437, 533
BmsI GCATC 1 cut(s) 499
BsaJI CCNNGG 1 cut(s) 436
BsaWI WCCGGW 1 cut(s) 470
Bsc4I CCNNNNNNNGG 1 cut(s) 488
Bse118I RCCGGY 1 cut(s) 470
Bse3DI GCAATG 2 cut(s) 20, 99
BseBI CCWGG 3 cut(s) 32, 437, 533
BseDI CCNNGG 1 cut(s) 436
BseLI CCNNNNNNNGG 1 cut(s) 488
BseMI GCAATG 2 cut(s) 20, 99
BshFI GGCC 1 cut(s) 536
BshTI ACCGGT 1 cut(s) 470
BsiHKAI GWGCWC 1 cut(s) 549
BsiSI CCGG 1 cut(s) 471
BslI CCNNNNNNNGG 1 cut(s) 488
BsmI GAATGC 1 cut(s) 63
BsnI GGCC 1 cut(s) 536
Bsp1286I GDGCHC 1 cut(s) 549
Bsp143I GATC 2 cut(s) 376, 477
BspACI CCGC 1 cut(s) 327
BspANI GGCC 1 cut(s) 536
BspLI GGNNCC 1 cut(s) 479
BspMI ACCTGC 1 cut(s) 91
BspPI GGATC 2 cut(s) 472, 485
BsrDI GCAATG 2 cut(s) 20, 99
BsrFI RCCGGY 1 cut(s) 470
BssAI RCCGGY 1 cut(s) 470
BssECI CCNNGG 1 cut(s) 436
BssMI GATC 2 cut(s) 376, 477
Bst2UI CCWGG 3 cut(s) 32, 437, 533
Bst4CI ACNGT 2 cut(s) 119, 363
Bst6I CTCTTC 1 cut(s) 218
BstC8I GCNNGC 1 cut(s) 188
BstHHI GCGC 2 cut(s) 100, 514
BstKTI GATC 2 cut(s) 379, 480
BstMBI GATC 2 cut(s) 376, 477
BstMWI GCNNNNNNNGC 1 cut(s) 520
BstNI CCWGG 3 cut(s) 32, 437, 533
BstNSI RCATGY 1 cut(s) 29
BstSCI CCNGG 3 cut(s) 30, 435, 531
BstX2I RGATCY 1 cut(s) 477
BstYI RGATCY 1 cut(s) 477
BsuRI GGCC 1 cut(s) 536
BveI ACCTGC 1 cut(s) 91
Cac8I GCNNGC 1 cut(s) 188
CfoI GCGC 2 cut(s) 100, 514
Cfr10I RCCGGY 1 cut(s) 470
Cfr13I GGNCC 2 cut(s) 34, 401
Csp6I GTAC 1 cut(s) 139
CspAI ACCGGT 1 cut(s) 470
CviAII CATG 1 cut(s) 26
CviJI RGCY 6 cut(s) 71, 165, 190, 287, 394, 536
CviKI_1 RGCY 6 cut(s) 71, 165, 190, 287, 394, 536
CviQI GTAC 1 cut(s) 139
DpnI GATC 2 cut(s) 378, 479
DpnII GATC 2 cut(s) 376, 477
Eam1104I CTCTTC 1 cut(s) 218
EarI CTCTTC 1 cut(s) 218
Eco147I AGGCCT 1 cut(s) 536
Eco47I GGWCC 2 cut(s) 34, 401
Eco57I CTGAAG 1 cut(s) 360
EcoRII CCWGG 3 cut(s) 30, 435, 531
FaeI CATG 1 cut(s) 29
FaiI YATR 6 cut(s) 27, 132, 156, 230, 323, 484
FalI AAGNNNNNCTT 4 cut(s) 197, 229, 406, 438
FatI CATG 1 cut(s) 25
FspBI CTAG 1 cut(s) 72
GlaI GCGC 2 cut(s) 99, 513
HaeIII GGCC 1 cut(s) 536
HapII CCGG 1 cut(s) 471
HhaI GCGC 2 cut(s) 100, 514
Hin1II CATG 1 cut(s) 29
Hin6I GCGC 2 cut(s) 98, 512
HinP1I GCGC 2 cut(s) 98, 512
HindIII AAGCTT 1 cut(s) 163
HinfI GANTC 2 cut(s) 368, 448
HpaII CCGG 1 cut(s) 471
HphI GGTGA 2 cut(s) 158, 460
Hpy166II GTNNAC 1 cut(s) 175
Hpy188I TCNGA 1 cut(s) 447
Hpy188III TCNNGA 5 cut(s) 240, 266, 302, 312, 338
Hpy8I GTNNAC 1 cut(s) 175
HpyAV CCTTC 1 cut(s) 335
HpyCH4III ACNGT 2 cut(s) 119, 363
HpyCH4IV ACGT 1 cut(s) 234
HpyCH4V TGCA 2 cut(s) 25, 41
HpyF10VI GCNNNNNNNGC 1 cut(s) 520
HpySE526I ACGT 1 cut(s) 234
Hsp92II CATG 1 cut(s) 29
HspAI GCGC 2 cut(s) 98, 512
Kzo9I GATC 2 cut(s) 376, 477
LmnI GCTCC 1 cut(s) 552
LweI GCATC 1 cut(s) 499
MaeI CTAG 1 cut(s) 72
MaeII ACGT 1 cut(s) 234
MaeIII GTNAC 2 cut(s) 329, 369
MalI GATC 2 cut(s) 378, 479
MboI GATC 2 cut(s) 376, 477
MboII GAAGA 3 cut(s) 170, 205, 281
MfeI CAATTG 1 cut(s) 248
MflI RGATCY 1 cut(s) 477
MhlI GDGCHC 1 cut(s) 549
MluCI AATT 6 cut(s) 10, 195, 248, 280, 405, 494
MlyI GAGTC 1 cut(s) 377
MnlI CCTC 4 cut(s) 201, 227, 420, 547
MseI TTAA 3 cut(s) 14, 179, 497
MspI CCGG 1 cut(s) 471
MspR9I CCNGG 3 cut(s) 32, 437, 533
MunI CAATTG 1 cut(s) 248
Mva1269I GAATGC 1 cut(s) 63
MvaI CCWGG 3 cut(s) 32, 437, 533
MwoI GCNNNNNNNGC 1 cut(s) 520
NdeII GATC 2 cut(s) 376, 477
NlaIII CATG 1 cut(s) 29
NlaIV GGNNCC 1 cut(s) 479
NmuCI GTSAC 2 cut(s) 329, 369
NspI RCATGY 1 cut(s) 29
PaqCI CACCTGC 1 cut(s) 91
PceI AGGCCT 1 cut(s) 536
PctI GAATGC 1 cut(s) 63
PfeI GAWTC 1 cut(s) 448
PfoI TCCNGGA 1 cut(s) 30
PinAI ACCGGT 1 cut(s) 470
PleI GAGTC 1 cut(s) 376
PpsI GAGTC 1 cut(s) 376
Psp6I CCWGG 3 cut(s) 30, 435, 531
PspGI CCWGG 3 cut(s) 30, 435, 531
PspN4I GGNNCC 1 cut(s) 479
PspPI GGNCC 2 cut(s) 34, 401
PsuI RGATCY 1 cut(s) 477
RsaI GTAC 1 cut(s) 140
RsaNI GTAC 1 cut(s) 139
SaqAI TTAA 3 cut(s) 14, 179, 497
Sau3AI GATC 2 cut(s) 376, 477
Sau96I GGNCC 2 cut(s) 34, 401
SchI GAGTC 1 cut(s) 377
ScrFI CCNGG 3 cut(s) 32, 437, 533
SduI GDGCHC 1 cut(s) 549
SetI ASST 7 cut(s) 39, 73, 105, 167, 237, 396, 403
SfaNI GCATC 1 cut(s) 499
SinI GGWCC 2 cut(s) 34, 401
Sse9I AATT 6 cut(s) 10, 195, 248, 280, 405, 494
SseBI AGGCCT 1 cut(s) 536
SsiI CCGC 1 cut(s) 327
SspMI CTAG 1 cut(s) 72
StuI AGGCCT 1 cut(s) 536
StyD4I CCNGG 3 cut(s) 30, 435, 531
TaaI ACNGT 2 cut(s) 119, 363
TaiI ACGT 1 cut(s) 237
TaqI TCGA 2 cut(s) 412, 507
TasI AATT 6 cut(s) 10, 195, 248, 280, 405, 494
TatI WGTACW 1 cut(s) 138
TfiI GAWTC 1 cut(s) 448
Tru1I TTAA 3 cut(s) 14, 179, 497
Tru9I TTAA 3 cut(s) 14, 179, 497
TseFI GTSAC 2 cut(s) 329, 369
Tsp45I GTSAC 2 cut(s) 329, 369
TspDTI ATGAA 2 cut(s) 9, 171
VpaK11BI GGWCC 2 cut(s) 34, 401
XapI RAATTY 2 cut(s) 10, 195
XceI RCATGY 1 cut(s) 29
XspI CTAG 1 cut(s) 72
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.