MD02G1312000.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0012261
Physical Location & Seq
Reverse (-)
36713433 .. 36722729
9297 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1312000.v1.1.491

Sequence Viewer

Length: 1011 bp
ATGGAGCATGATCAGGACCAAGAAGCTCTATTTCATTCATACCCTTGTGCATACTACGTGCAAAGCCCATCCACCAACTCCCATTCCAACAGTGCTGATATCCGAAACCCCATCCCGGAATCTGAATTCCACTCCCCAACACGACCCGATCCAGCAAACCCAACCCTTCATGAAGAGGCCACTCGCTTCACTCTCTCGCACTACTTGTCCTCCCGTGGATCCAGTCACTCATTCCTGCACCAAAAAAAGATCTCCTTTGATGCACGTAGTAATGGCACAGGGACCGATAATTGCGAAAACCGTCTGGTTATCGTCAAAGGTAGTGATGATCACTTTGGCGATGAAGATGATGACGAGGAGTATTATTATGTGGTTGGGAAAAGATCGGGGTGGTGGAAGAGGTATTGTTCATATCGATATTCGGATTCGTTTGTATGGATACTTTTGCAAATAAGTTGGAGGGCTATGTTGAGTTTGTGTATCGCACTGCTTGTGTTTTACGTTGTTACAAAGCCCCCACCACCCAAAGTGTCTATTCAGATGGCAGGAATTGACCAATTTGGGCTTGGGGAAGGAGTGGATGGCTCGGGCGTCACAACTAAGTTCCTTACCTGCAATTGTTCCATAAATCTCATAATAGAGAACAAGTCTAAGCTCTTTAGCCTCCACATTCGTCCTCCAGCGATGGAAATGTGCTTCGGCCGGCTACCTTTTGCACTATCACATGGACCAAAGCTATATGCTGAGAGTGGGTCATCAAAATTCAAGTTATATGTTGGAACAAGGAATAAGCCTGTGTATGGTGCTGGAAGAAGCATGGACGATATGCTCAAATCAGGGAAGGGTTTGCCGTTGGTGCTTCGTGTGAAACTGAGCTCAAGTTTTTGGGTCGTTAGGAATCTCATAAAACCTAAATTTCATCACCAAGCAGAATGTTTGTTGGTCCTTGATAGGGCATATGACAAAAAGCATCGTACTCAGGCATACAGTAGCACCTGCATGATGACTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

337

Amino Acids

38.05

Weight (kDa)

8.61

Isoelectric Point (pI)

40.37

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012261)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G08490
fragaria_vesca FvH4_7g01030 FvH4_7g01030 FvH4_7g01030
malus_domestica MD02G1312000.v1.1 MD07G1010600.v1.1
prunus_persica Prupe.2G012900_v2.0.a1
pyrus_communis pycom07g00870
rosa_chinensis RchiOBHm_Chr1g0315961
rosa_laevigata RLG00000030720
rosa_multiflora Rmu_sc0004566.1_g000003 Rmu_sc0005954.1_g000042
rosa_roxburghii Rroxscaffold_4G00331290
rosa_rugosa Rorug01G0007200
rosa_samantha Rh1AG018000 Rh1BG011000 Rh1CG015300 Rh1DG011100
rosa_wichuraiana Rw1G001100

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1004
Acc36I ACCTGC 2 cut(s) 620, 1004
AclWI GGATC 3 cut(s) 143, 213, 226
AcoI YGGCCR 1 cut(s) 700
AcsI RAATTY 3 cut(s) 125, 761, 914
AcyI GRCGYC 1 cut(s) 591
AfaI GTAC 1 cut(s) 976
AfiI CCNNNNNNNGG 3 cut(s) 115, 800, 952
AgsI TTSAA 1 cut(s) 766
AluBI AGCT 4 cut(s) 26, 655, 736, 876
AluI AGCT 4 cut(s) 26, 655, 736, 876
Alw21I GWGCWC 1 cut(s) 878
AlwI GGATC 3 cut(s) 143, 213, 226
Ama87I CYCGRG 1 cut(s) 586
AoxI GGCC 2 cut(s) 177, 700
ApoI RAATTY 3 cut(s) 125, 761, 914
AspS9I GGNCC 4 cut(s) 16, 282, 728, 943
AsuC2I CCSGG 1 cut(s) 116
AsuHPI GGTGA 1 cut(s) 914
AvaI CYCGRG 1 cut(s) 586
AvaII GGWCC 4 cut(s) 16, 282, 728, 943
BamHI GGATCC 1 cut(s) 218
BanII GRGCYC 1 cut(s) 878
Bbv12I GWGCWC 1 cut(s) 878
BccI CCATC 5 cut(s) 76, 119, 535, 575, 679
BceAI ACGGC 1 cut(s) 835
BciVI GTATCC 1 cut(s) 432
BclI TGATCA 2 cut(s) 10, 328
BcnI CCSGG 1 cut(s) 116
BfuAI ACCTGC 2 cut(s) 620, 1004
BfuI GTATCC 1 cut(s) 432
BglII AGATCT 1 cut(s) 249
Bme1390I CCNGG 1 cut(s) 116
Bme18I GGWCC 4 cut(s) 16, 282, 728, 943
BmeT110I CYCGRG 1 cut(s) 586
BmgT120I GGNCC 4 cut(s) 16, 282, 728, 943
BmiI GGNNCC 2 cut(s) 220, 283
BmrFI CCNGG 1 cut(s) 116
BmsI GCATC 2 cut(s) 250, 979
BpmI CTGGAG 1 cut(s) 663
BpuEI CTTGAG 1 cut(s) 862
BpuMI CCSGG 1 cut(s) 116
Bsa29I ATCGAT 1 cut(s) 415
BsaAI YACGTR 2 cut(s) 58, 266
BsaHI GRCGYC 1 cut(s) 591
BsaJI CCNNGG 1 cut(s) 214
BsaXI ACNNNNNCTCC 2 cut(s) 194, 224
Bsc4I CCNNNNNNNGG 3 cut(s) 115, 800, 952
Bse118I RCCGGY 1 cut(s) 702
Bse1I ACTGG 1 cut(s) 222
BseCI ATCGAT 1 cut(s) 415
BseDI CCNNGG 1 cut(s) 214
BseGI GGATG 3 cut(s) 68, 111, 586
BseLI CCNNNNNNNGG 3 cut(s) 115, 800, 952
BseMII CTCAG 3 cut(s) 735, 863, 992
BseNI ACTGG 1 cut(s) 222
BseRI GAGGAG 1 cut(s) 371
BseX3I CGGCCG 1 cut(s) 700
BsgI GTGCAG 1 cut(s) 221
Bsh1285I CGRYCG 1 cut(s) 703
BshFI GGCC 2 cut(s) 179, 702
BshVI ATCGAT 1 cut(s) 415
BsiEI CGRYCG 1 cut(s) 703
BsiHKAI GWGCWC 1 cut(s) 878
BsiHKCI CYCGRG 1 cut(s) 586
BsiSI CCGG 2 cut(s) 116, 703
BslFI GGGAC 1 cut(s) 295
BslI CCNNNNNNNGG 3 cut(s) 115, 800, 952
BsmFI GGGAC 1 cut(s) 295
BsnI GGCC 2 cut(s) 179, 702
BsoBI CYCGRG 1 cut(s) 586
Bsp1286I GDGCHC 1 cut(s) 878
Bsp143I GATC 6 cut(s) 10, 148, 218, 249, 328, 383
BspANI GGCC 2 cut(s) 179, 702
BspCNI CTCAG 3 cut(s) 736, 864, 991
BspDI ATCGAT 1 cut(s) 415
BspHI TCATGA 1 cut(s) 169
BspLI GGNNCC 2 cut(s) 220, 283
BspMI ACCTGC 2 cut(s) 620, 1004
BspPI GGATC 3 cut(s) 143, 213, 226
BsrFI RCCGGY 1 cut(s) 702
BsrI ACTGG 1 cut(s) 222
BssAI RCCGGY 1 cut(s) 702
BssECI CCNNGG 1 cut(s) 214
BssMI GATC 6 cut(s) 10, 148, 218, 249, 328, 383
BssNI GRCGYC 1 cut(s) 591
Bst4CI ACNGT 3 cut(s) 92, 302, 989
Bst6I CTCTTC 2 cut(s) 168, 392
BstACI GRCGYC 1 cut(s) 591
BstBAI YACGTR 2 cut(s) 58, 266
BstC8I GCNNGC 1 cut(s) 704
BstDEI CTNAG 5 cut(s) 600, 651, 744, 872, 978
BstDSI CCRYGG 1 cut(s) 214
BstF5I GGATG 3 cut(s) 68, 111, 586
BstKTI GATC 6 cut(s) 13, 151, 221, 252, 331, 386
BstMBI GATC 6 cut(s) 10, 148, 218, 249, 328, 383
BstMCI CGRYCG 1 cut(s) 703
BstMWI GCNNNNNNNGC 1 cut(s) 856
BstSCI CCNGG 1 cut(s) 114
BstX2I RGATCY 2 cut(s) 218, 249
BstYI RGATCY 2 cut(s) 218, 249
BstZI CGGCCG 1 cut(s) 700
Bsu15I ATCGAT 1 cut(s) 415
BsuI GTATCC 1 cut(s) 432
BsuRI GGCC 2 cut(s) 179, 702
BsuTUI ATCGAT 1 cut(s) 415
BtgI CCRYGG 1 cut(s) 214
BtgZI GCGATG 2 cut(s) 354, 698
BtsCI GGATG 3 cut(s) 68, 111, 586
BtsI GCAGTG 1 cut(s) 485
BtsIMutI CAGTG 2 cut(s) 97, 485
BveI ACCTGC 2 cut(s) 620, 1004
Cac8I GCNNGC 1 cut(s) 704
CciI TCATGA 1 cut(s) 169
Cfr10I RCCGGY 1 cut(s) 702
Cfr13I GGNCC 4 cut(s) 16, 282, 728, 943
ClaI ATCGAT 1 cut(s) 415
CseI GACGC 1 cut(s) 580
Csp6I GTAC 1 cut(s) 975
CviAII CATG 5 cut(s) 8, 170, 725, 817, 1000
CviQI GTAC 1 cut(s) 975
DdeI CTNAG 5 cut(s) 600, 651, 744, 872, 978
DpnI GATC 6 cut(s) 12, 150, 220, 251, 330, 385
DpnII GATC 6 cut(s) 10, 148, 218, 249, 328, 383
EaeI YGGCCR 1 cut(s) 700
EagI CGGCCG 1 cut(s) 700
Eam1104I CTCTTC 2 cut(s) 168, 392
EarI CTCTTC 2 cut(s) 168, 392
Ecl136II GAGCTC 1 cut(s) 876
EclXI CGGCCG 1 cut(s) 700
Eco24I GRGCYC 1 cut(s) 878
Eco32I GATATC 1 cut(s) 100
Eco47I GGWCC 4 cut(s) 16, 282, 728, 943
Eco52I CGGCCG 1 cut(s) 700
Eco53kI GAGCTC 1 cut(s) 876
Eco88I CYCGRG 1 cut(s) 586
EcoICRI GAGCTC 1 cut(s) 876
EcoRI GAATTC 1 cut(s) 125
EcoRV GATATC 1 cut(s) 100
EcoT38I GRGCYC 1 cut(s) 878
FaeI CATG 5 cut(s) 11, 173, 728, 820, 1003
FalI AAGNNNNNCTT 2 cut(s) 239, 271
FaqI GGGAC 1 cut(s) 295
FatI CATG 5 cut(s) 7, 169, 724, 816, 999
FauNDI CATATG 1 cut(s) 958
FbaI TGATCA 2 cut(s) 10, 328
FokI GGATG 3 cut(s) 55, 98, 593
FriOI GRGCYC 1 cut(s) 878
GsuI CTGGAG 1 cut(s) 663
HaeIII GGCC 2 cut(s) 179, 702
HapII CCGG 2 cut(s) 116, 703
HgaI GACGC 1 cut(s) 580
Hin1I GRCGYC 1 cut(s) 591
Hin1II CATG 5 cut(s) 11, 173, 728, 820, 1003
HinfI GANTC 3 cut(s) 119, 425, 898
HpaII CCGG 2 cut(s) 116, 703
HphI GGTGA 1 cut(s) 914
Hpy188I TCNGA 4 cut(s) 104, 124, 424, 540
Hpy188III TCNNGA 2 cut(s) 14, 170
HpyAV CCTTC 3 cut(s) 176, 566, 835
HpyCH4III ACNGT 3 cut(s) 92, 302, 989
HpyCH4IV ACGT 3 cut(s) 57, 265, 501
HpyCH4V TGCA 8 cut(s) 50, 61, 238, 263, 448, 615, 716, 999
HpyF10VI GCNNNNNNNGC 1 cut(s) 856
HpyF3I CTNAG 5 cut(s) 600, 651, 744, 872, 978
HpySE526I ACGT 3 cut(s) 57, 265, 501
Hsp92I GRCGYC 1 cut(s) 591
Hsp92II CATG 5 cut(s) 11, 173, 728, 820, 1003
KroI GCCGGC 1 cut(s) 702
KroNI GCCGGC 1 cut(s) 704
Ksp22I TGATCA 2 cut(s) 10, 328
Kzo9I GATC 6 cut(s) 10, 148, 218, 249, 328, 383
LmnI GCTCC 1 cut(s) 4
LweI GCATC 2 cut(s) 250, 979
MaeII ACGT 3 cut(s) 57, 265, 501
MaeIII GTNAC 3 cut(s) 224, 505, 592
MalI GATC 6 cut(s) 12, 150, 220, 251, 330, 385
MboI GATC 6 cut(s) 10, 148, 218, 249, 328, 383
MboII GAAGA 4 cut(s) 185, 356, 409, 822
MfeI CAATTG 1 cut(s) 616
MflI RGATCY 2 cut(s) 218, 249
MhlI GDGCHC 1 cut(s) 878
MluCI AATT 7 cut(s) 125, 289, 549, 557, 616, 761, 914
MmeI TCCRAC 3 cut(s) 111, 437, 757
MnlI CCTC 7 cut(s) 169, 220, 349, 393, 453, 674, 687
MroNI GCCGGC 1 cut(s) 702
MslI CAYNNNNRTG 1 cut(s) 998
MspI CCGG 2 cut(s) 116, 703
MspR9I CCNGG 1 cut(s) 116
MunI CAATTG 1 cut(s) 616
MwoI GCNNNNNNNGC 1 cut(s) 856
NaeI GCCGGC 1 cut(s) 704
NciI CCSGG 1 cut(s) 116
NdeI CATATG 1 cut(s) 958
NdeII GATC 6 cut(s) 10, 148, 218, 249, 328, 383
NgoMIV GCCGGC 1 cut(s) 702
NlaIII CATG 5 cut(s) 11, 173, 728, 820, 1003
NlaIV GGNNCC 2 cut(s) 220, 283
NmuCI GTSAC 2 cut(s) 224, 592
PagI TCATGA 1 cut(s) 169
PaqCI CACCTGC 1 cut(s) 1004
PdiI GCCGGC 1 cut(s) 704
PfeI GAWTC 3 cut(s) 119, 425, 898
PfoI TCCNGGA 1 cut(s) 114
Ppu21I YACGTR 2 cut(s) 58, 266
Psp124BI GAGCTC 1 cut(s) 878
PspN4I GGNNCC 2 cut(s) 220, 283
PspPI GGNCC 4 cut(s) 16, 282, 728, 943
PsuI RGATCY 2 cut(s) 218, 249
RsaI GTAC 1 cut(s) 976
RsaNI GTAC 1 cut(s) 975
RseI CAYNNNNRTG 1 cut(s) 998
SacI GAGCTC 1 cut(s) 878
Sau3AI GATC 6 cut(s) 10, 148, 218, 249, 328, 383
Sau96I GGNCC 4 cut(s) 16, 282, 728, 943
ScrFI CCNGG 1 cut(s) 116
SduI GDGCHC 1 cut(s) 878
SfaNI GCATC 2 cut(s) 250, 979
SinI GGWCC 4 cut(s) 16, 282, 728, 943
SmiMI CAYNNNNRTG 1 cut(s) 998
SmlI CTYRAG 1 cut(s) 877
SmoI CTYRAG 1 cut(s) 877
Sse9I AATT 7 cut(s) 125, 289, 549, 557, 616, 761, 914
SstI GAGCTC 1 cut(s) 878
StyD4I CCNGG 1 cut(s) 114
TaaI ACNGT 3 cut(s) 92, 302, 989
TaiI ACGT 3 cut(s) 60, 268, 504
TaqI TCGA 1 cut(s) 415
TaqII GACCGA 1 cut(s) 299
TasI AATT 7 cut(s) 125, 289, 549, 557, 616, 761, 914
TfiI GAWTC 3 cut(s) 119, 425, 898
TscAI CASTG 2 cut(s) 97, 492
TseFI GTSAC 2 cut(s) 224, 592
Tsp45I GTSAC 2 cut(s) 224, 592
TspDTI ATGAA 7 cut(s) 23, 27, 158, 186, 357, 399, 908
TspRI CASTG 2 cut(s) 97, 492
VpaK11BI GGWCC 4 cut(s) 16, 282, 728, 943
XapI RAATTY 3 cut(s) 125, 761, 914
XcmI CCANNNNNNNNNTGG 1 cut(s) 563
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.