Rroxscaffold_4G00331290

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0012261
Physical Location & Seq
Reverse (-)
65192269 .. 65195440
3172 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00331290.1

Sequence Viewer

Length: 1050 bp
ATGTCACAGCTCAGAAATAAAATGGAGCATGATCATGAAGCAGCTCTATTCCATTCATACCCTGCCTGTGCATACTACGTCCAGAGCCCATCCACCATCTCCCATGCTAACAGCGCCCATATCCGAAACAGCACCATCCCCGAATCCGAATTCCAATTCAACTCCCCCCGACCCGACGCAACAAACCCTACCCTTCACGAGCAGGAAGCAGCCTCTCGAGCCTTCATTCTCTCGCACTACTCCTCCTCCCGCGGCTCCAGCCACTCCTTCTTGCATCACCAGAAAAAGATCTCTTACGACGCAGGTAGCCACGAAAATGGCGAGAACCGTTTGATTATTGTGGATGCCGGCGGCGATGATATTCATAATGGTGATGGTCGTGACGATGATGATGAATATGATTATTACGGGATGAAGAAAAGATCAGGGTGGTGGAAGAGGTACTGTTCGTATAGGAATTCGGACTCGTGTGTGTGGATTTTCATGCAAATTAGTTGGAGGGCTATCTTGAGCTTCTGGGTTGCATTGCTTGTGTTTTACATCGCCACAAAGCCACCTCCACCCAAAGTATCTATTAAGATGGCAGGAATTGGGCAATTTGGGTTAGGGGAAGGAGTTGATGGTTCAGGTGTTTCAACAAAGTTCCTAACCTGCAATTGTTCCATAGACCTAATAATAGAGAACAAGTCTAAGCTCTTTGGACTTCAAATTCATCCTCCAGTGATGGACGTCTCCTTCGGCCGCCTGTCTTTTGCATCATCACATGGACCAAAGCTATATGCTGAACATGGGTCAACAAAGTTCAAGTTATATGTGGGAACAAGAAACAGGCCTATGTATGGTGCTGGAAGAAGCATGGAGGACATGTTAGAGTCAGGCAAGGGTTTGCCTTTGGTGGTTCGAGTCAGATTGCGCTCAAGTTTTCAAGTCGTTTGGAATCTGATAAAGCCTAAGTTCCATCACCAAGCTCAGTGCTCATTAGTCCTTGATCGGGCTTACGACAGAAGGCATCGAACTCAGGCCTACAACAGCACCTGCATAATCACTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

349

Amino Acids

39.45

Weight (kDa)

8.94

Isoelectric Point (pI)

46.36

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012261)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G08490
fragaria_vesca FvH4_7g01030 FvH4_7g01030 FvH4_7g01030
malus_domestica MD02G1312000.v1.1 MD07G1010600.v1.1
prunus_persica Prupe.2G012900_v2.0.a1
pyrus_communis pycom07g00870
rosa_chinensis RchiOBHm_Chr1g0315961
rosa_laevigata RLG00000030720
rosa_multiflora Rmu_sc0004566.1_g000003 Rmu_sc0005954.1_g000042
rosa_roxburghii Rroxscaffold_4G00331290
rosa_rugosa Rorug01G0007200
rosa_samantha Rh1AG018000 Rh1BG011000 Rh1CG015300 Rh1DG011100
rosa_wichuraiana Rw1G001100

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1043
AatII GACGTC 1 cut(s) 732
Acc36I ACCTGC 3 cut(s) 293, 659, 1043
AccII CGCG 1 cut(s) 252
AciI CCGC 4 cut(s) 250, 252, 351, 742
AcoI YGGCCR 1 cut(s) 739
AcsI RAATTY 3 cut(s) 149, 457, 708
AcyI GRCGYC 1 cut(s) 729
AfaI GTAC 1 cut(s) 443
AfiI CCNNNNNNNGG 2 cut(s) 839, 991
AflIII ACRYGT 1 cut(s) 864
AgsI TTSAA 5 cut(s) 160, 636, 707, 805, 926
AluBI AGCT 6 cut(s) 10, 44, 513, 694, 775, 968
AluI AGCT 6 cut(s) 10, 44, 513, 694, 775, 968
Alw21I GWGCWC 1 cut(s) 977
Alw26I GTCTC 1 cut(s) 736
AlwNI CAGNNNCTG 1 cut(s) 1035
Ama87I CYCGRG 1 cut(s) 216
AoxI GGCC 3 cut(s) 739, 830, 1020
ApeKI GCWGC 2 cut(s) 41, 209
ApoI RAATTY 3 cut(s) 149, 457, 708
ArsI GACNNNNNNTTYG 2 cut(s) 719, 751
AspLEI GCGC 2 cut(s) 116, 915
AspS9I GGNCC 1 cut(s) 767
AsuHPI GGTGA 3 cut(s) 269, 383, 953
AvaI CYCGRG 1 cut(s) 216
AvaII GGWCC 1 cut(s) 767
BanII GRGCYC 1 cut(s) 89
BauI CACGAG 2 cut(s) 197, 466
Bbv12I GWGCWC 1 cut(s) 977
BbvI GCAGC 2 cut(s) 53, 221
BccI CCATC 8 cut(s) 97, 104, 143, 368, 574, 614, 718, 966
BclI TGATCA 1 cut(s) 31
BcoDI GTCTC 1 cut(s) 736
BfoI RGCGCY 1 cut(s) 117
BfuAI ACCTGC 3 cut(s) 293, 659, 1043
BglII AGATCT 1 cut(s) 288
BisI GCNGC 5 cut(s) 42, 210, 253, 352, 742
BlsI GCNGC 5 cut(s) 43, 211, 254, 353, 743
Bme18I GGWCC 1 cut(s) 767
BmeT110I CYCGRG 1 cut(s) 216
BmgT120I GGNCC 1 cut(s) 767
BmiI GGNNCC 1 cut(s) 256
BmsI GCATC 4 cut(s) 283, 334, 764, 1018
BpmI CTGGAG 2 cut(s) 241, 702
BpuEI CTTGAG 2 cut(s) 529, 901
BsaHI GRCGYC 1 cut(s) 729
BsaJI CCNNGG 1 cut(s) 250
BsaXI ACNNNNNCTCC 6 cut(s) 227, 230, 257, 260, 851, 881
Bsc4I CCNNNNNNNGG 2 cut(s) 839, 991
Bse118I RCCGGY 1 cut(s) 347
Bse1I ACTGG 1 cut(s) 719
Bse3DI GCAATG 1 cut(s) 524
BseDI CCNNGG 1 cut(s) 250
BseGI GGATG 5 cut(s) 89, 135, 349, 417, 712
BseLI CCNNNNNNNGG 2 cut(s) 839, 991
BseMI GCAATG 1 cut(s) 524
BseMII CTCAG 3 cut(s) 25, 983, 1031
BseNI ACTGG 1 cut(s) 719
BseRI GAGGAG 2 cut(s) 232, 235
BseX3I CGGCCG 1 cut(s) 739
BseXI GCAGC 2 cut(s) 53, 221
Bsh1236I CGCG 1 cut(s) 252
Bsh1285I CGRYCG 1 cut(s) 742
BshFI GGCC 3 cut(s) 741, 832, 1022
BsiEI CGRYCG 1 cut(s) 742
BsiHKAI GWGCWC 1 cut(s) 977
BsiHKCI CYCGRG 1 cut(s) 216
BsiSI CCGG 1 cut(s) 348
BslI CCNNNNNNNGG 2 cut(s) 839, 991
BsmAI GTCTC 1 cut(s) 736
BsmBI CGTCTC 1 cut(s) 736
BsnI GGCC 3 cut(s) 741, 832, 1022
BsoBI CYCGRG 1 cut(s) 216
Bsp1286I GDGCHC 2 cut(s) 89, 977
Bsp143I GATC 4 cut(s) 31, 288, 422, 988
BspACI CCGC 4 cut(s) 250, 252, 351, 742
BspANI GGCC 3 cut(s) 741, 832, 1022
BspCNI CTCAG 3 cut(s) 24, 982, 1030
BspFNI CGCG 1 cut(s) 252
BspHI TCATGA 1 cut(s) 34
BspLI GGNNCC 1 cut(s) 256
BspMI ACCTGC 3 cut(s) 293, 659, 1043
BsrDI GCAATG 1 cut(s) 524
BsrFI RCCGGY 1 cut(s) 347
BsrI ACTGG 1 cut(s) 719
BssAI RCCGGY 1 cut(s) 347
BssECI CCNNGG 1 cut(s) 250
BssMI GATC 4 cut(s) 31, 288, 422, 988
BssNI GRCGYC 1 cut(s) 729
BssSI CACGAG 2 cut(s) 197, 466
Bst2BI CACGAG 2 cut(s) 197, 466
Bst4CI ACNGT 2 cut(s) 329, 446
Bst6I CTCTTC 1 cut(s) 431
BstACI GRCGYC 1 cut(s) 729
BstC8I GCNNGC 1 cut(s) 349
BstDEI CTNAG 5 cut(s) 11, 690, 951, 969, 1017
BstDSI CCRYGG 1 cut(s) 250
BstF5I GGATG 5 cut(s) 89, 135, 349, 417, 712
BstFNI CGCG 1 cut(s) 252
BstH2I RGCGCY 1 cut(s) 117
BstHHI GCGC 2 cut(s) 116, 915
BstKTI GATC 4 cut(s) 34, 291, 425, 991
BstMAI GTCTC 1 cut(s) 736
BstMBI GATC 4 cut(s) 31, 288, 422, 988
BstMCI CGRYCG 1 cut(s) 742
BstMWI GCNNNNNNNGC 3 cut(s) 113, 218, 258
BstNSI RCATGY 1 cut(s) 868
BstUI CGCG 1 cut(s) 252
BstV1I GCAGC 2 cut(s) 53, 221
BstX2I RGATCY 1 cut(s) 288
BstXI CCANNNNNNTGG 1 cut(s) 317
BstYI RGATCY 1 cut(s) 288
BstZI CGGCCG 1 cut(s) 739
BsuRI GGCC 3 cut(s) 741, 832, 1022
BtgI CCRYGG 1 cut(s) 250
BtgZI GCGATG 2 cut(s) 369, 526
BtsCI GGATG 5 cut(s) 89, 135, 349, 417, 712
BtsIMutI CAGTG 2 cut(s) 726, 977
BveI ACCTGC 3 cut(s) 293, 659, 1043
Cac8I GCNNGC 1 cut(s) 349
CaiI CAGNNNCTG 1 cut(s) 1035
CciI TCATGA 1 cut(s) 34
CfoI GCGC 2 cut(s) 116, 915
Cfr10I RCCGGY 1 cut(s) 347
Cfr13I GGNCC 1 cut(s) 767
Cfr42I CCGCGG 1 cut(s) 253
CseI GACGC 2 cut(s) 185, 308
Csp6I GTAC 1 cut(s) 442
CviAII CATG 8 cut(s) 29, 35, 104, 484, 764, 788, 856, 865
CviQI GTAC 1 cut(s) 442
DdeI CTNAG 5 cut(s) 11, 690, 951, 969, 1017
DpnI GATC 4 cut(s) 33, 290, 424, 990
DpnII GATC 4 cut(s) 31, 288, 422, 988
EaeI YGGCCR 1 cut(s) 739
EagI CGGCCG 1 cut(s) 739
Eam1104I CTCTTC 1 cut(s) 431
EarI CTCTTC 1 cut(s) 431
EclXI CGGCCG 1 cut(s) 739
Eco147I AGGCCT 2 cut(s) 832, 1022
Eco24I GRGCYC 1 cut(s) 89
Eco47I GGWCC 1 cut(s) 767
Eco52I CGGCCG 1 cut(s) 739
Eco88I CYCGRG 1 cut(s) 216
EcoRI GAATTC 2 cut(s) 149, 457
EcoT38I GRGCYC 1 cut(s) 89
Esp3I CGTCTC 1 cut(s) 736
FaeI CATG 8 cut(s) 32, 38, 107, 487, 767, 791, 859, 868
FatI CATG 8 cut(s) 28, 34, 103, 483, 763, 787, 855, 864
FauI CCCGC 1 cut(s) 257
FbaI TGATCA 1 cut(s) 31
Fnu4HI GCNGC 5 cut(s) 42, 210, 253, 352, 742
FokI GGATG 5 cut(s) 76, 122, 356, 424, 699
FriOI GRGCYC 1 cut(s) 89
Fsp4HI GCNGC 5 cut(s) 42, 210, 253, 352, 742
GlaI GCGC 2 cut(s) 115, 914
GluI GCNGC 5 cut(s) 42, 210, 253, 352, 742
GsuI CTGGAG 2 cut(s) 241, 702
HaeII RGCGCY 1 cut(s) 117
HaeIII GGCC 3 cut(s) 741, 832, 1022
HapII CCGG 1 cut(s) 348
HgaI GACGC 2 cut(s) 185, 308
HhaI GCGC 2 cut(s) 116, 915
Hin1I GRCGYC 1 cut(s) 729
Hin1II CATG 8 cut(s) 32, 38, 107, 487, 767, 791, 859, 868
Hin6I GCGC 2 cut(s) 114, 913
HinP1I GCGC 2 cut(s) 114, 913
HincII GTYRAC 1 cut(s) 795
HindII GTYRAC 1 cut(s) 795
HinfI GANTC 5 cut(s) 143, 464, 872, 903, 937
HpaII CCGG 1 cut(s) 348
HphI GGTGA 3 cut(s) 269, 383, 953
Hpy166II GTNNAC 1 cut(s) 795
Hpy188I TCNGA 6 cut(s) 14, 125, 148, 463, 908, 942
Hpy188III TCNNGA 6 cut(s) 35, 82, 197, 216, 380, 508
Hpy8I GTNNAC 1 cut(s) 795
Hpy99I CGWCG 2 cut(s) 179, 302
HpyAV CCTTC 6 cut(s) 203, 232, 277, 605, 745, 999
HpyCH4III ACNGT 2 cut(s) 329, 446
HpyCH4IV ACGT 2 cut(s) 78, 729
HpyCH4V TGCA 7 cut(s) 71, 274, 487, 524, 654, 755, 1038
HpyF10VI GCNNNNNNNGC 3 cut(s) 113, 218, 258
HpyF3I CTNAG 5 cut(s) 11, 690, 951, 969, 1017
HpySE526I ACGT 2 cut(s) 78, 729
Hsp92I GRCGYC 1 cut(s) 729
Hsp92II CATG 8 cut(s) 32, 38, 107, 487, 767, 791, 859, 868
HspAI GCGC 2 cut(s) 114, 913
KroI GCCGGC 1 cut(s) 347
KroNI GCCGGC 1 cut(s) 349
Ksp22I TGATCA 1 cut(s) 31
KspI CCGCGG 1 cut(s) 253
Kzo9I GATC 4 cut(s) 31, 288, 422, 988
LmnI GCTCC 2 cut(s) 25, 260
Lsp1109I GCAGC 2 cut(s) 53, 221
LweI GCATC 4 cut(s) 283, 334, 764, 1018
MaeII ACGT 2 cut(s) 78, 729
MaeIII GTNAC 2 cut(s) 3, 380
MalI GATC 4 cut(s) 33, 290, 424, 990
MboI GATC 4 cut(s) 31, 288, 422, 988
MboII GAAGA 3 cut(s) 427, 448, 861
MfeI CAATTG 1 cut(s) 655
MflI RGATCY 1 cut(s) 288
MhlI GDGCHC 2 cut(s) 89, 977
MluCI AATT 8 cut(s) 149, 155, 457, 489, 588, 596, 655, 708
MlyI GAGTC 3 cut(s) 458, 881, 912
MmeI TCCRAC 1 cut(s) 476
MnlI CCTC 8 cut(s) 223, 253, 256, 432, 492, 567, 726, 853
MroNI GCCGGC 1 cut(s) 347
MseI TTAA 2 cut(s) 576, 1048
MslI CAYNNNNRTG 3 cut(s) 33, 315, 369
MspA1I CMGCKG 1 cut(s) 252
MspI CCGG 1 cut(s) 348
MunI CAATTG 1 cut(s) 655
MvnI CGCG 1 cut(s) 252
MwoI GCNNNNNNNGC 3 cut(s) 113, 218, 258
NaeI GCCGGC 1 cut(s) 349
NdeII GATC 4 cut(s) 31, 288, 422, 988
NgoMIV GCCGGC 1 cut(s) 347
NlaIII CATG 8 cut(s) 32, 38, 107, 487, 767, 791, 859, 868
NlaIV GGNNCC 1 cut(s) 256
NmuCI GTSAC 2 cut(s) 3, 380
NspI RCATGY 1 cut(s) 868
PaeR7I CTCGAG 1 cut(s) 216
PagI TCATGA 1 cut(s) 34
PaqCI CACCTGC 1 cut(s) 1043
PceI AGGCCT 2 cut(s) 832, 1022
PciI ACATGT 1 cut(s) 864
PcsI WCGNNNNNNNCGW 1 cut(s) 318
PdiI GCCGGC 1 cut(s) 349
PfeI GAWTC 2 cut(s) 143, 937
PkrI GCNGC 5 cut(s) 43, 211, 254, 353, 743
PleI GAGTC 3 cut(s) 458, 880, 911
PpsI GAGTC 3 cut(s) 458, 880, 911
PscI ACATGT 1 cut(s) 864
PspN4I GGNNCC 1 cut(s) 256
PspPI GGNCC 1 cut(s) 767
PstNI CAGNNNCTG 1 cut(s) 1035
PsuI RGATCY 1 cut(s) 288
RsaI GTAC 1 cut(s) 443
RsaNI GTAC 1 cut(s) 442
RseI CAYNNNNRTG 3 cut(s) 33, 315, 369
SacII CCGCGG 1 cut(s) 253
SaqAI TTAA 2 cut(s) 576, 1048
SatI GCNGC 5 cut(s) 42, 210, 253, 352, 742
Sau3AI GATC 4 cut(s) 31, 288, 422, 988
Sau96I GGNCC 1 cut(s) 767
SchI GAGTC 3 cut(s) 458, 881, 912
SduI GDGCHC 2 cut(s) 89, 977
SfaNI GCATC 4 cut(s) 283, 334, 764, 1018
Sfr274I CTCGAG 1 cut(s) 216
Sfr303I CCGCGG 1 cut(s) 253
SgrBI CCGCGG 1 cut(s) 253
SinI GGWCC 1 cut(s) 767
SlaI CTCGAG 1 cut(s) 216
SmiMI CAYNNNNRTG 3 cut(s) 33, 315, 369
SmlI CTYRAG 3 cut(s) 216, 508, 916
SmoI CTYRAG 3 cut(s) 216, 508, 916
Sse9I AATT 8 cut(s) 149, 155, 457, 489, 588, 596, 655, 708
SseBI AGGCCT 2 cut(s) 832, 1022
SsiI CCGC 4 cut(s) 250, 252, 351, 742
StuI AGGCCT 2 cut(s) 832, 1022
TaaI ACNGT 2 cut(s) 329, 446
TaiI ACGT 2 cut(s) 81, 732
TaqI TCGA 3 cut(s) 217, 901, 1012
TasI AATT 8 cut(s) 149, 155, 457, 489, 588, 596, 655, 708
TauI GCSGC 3 cut(s) 255, 354, 744
TfiI GAWTC 2 cut(s) 143, 937
Tru1I TTAA 2 cut(s) 576, 1048
Tru9I TTAA 2 cut(s) 576, 1048
TscAI CASTG 2 cut(s) 726, 977
TseFI GTSAC 2 cut(s) 3, 380
TseI GCWGC 2 cut(s) 41, 209
Tsp45I GTSAC 2 cut(s) 3, 380
TspDTI ATGAA 8 cut(s) 45, 51, 214, 353, 408, 428, 472, 701
TspRI CASTG 2 cut(s) 726, 977
VpaK11BI GGWCC 1 cut(s) 767
XapI RAATTY 3 cut(s) 149, 457, 708
XceI RCATGY 1 cut(s) 868
XhoI CTCGAG 1 cut(s) 216
ZraI GACGTC 1 cut(s) 730
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.