Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr03

Orthogroup ID

OG0005676

Physical Location & Seq

Genomic Coordinates Forward (+)

942712 .. 946931

Gene Structure 4220 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD03G1012400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2052 bp

ATGCTCCTCAGAACCCAATCTCTATCGCTCTTCATCTTCAACCAATCAACAACCGAAACCAGAGCGCATAAAACCCAAGCATTTCAGATCAAACCCACCAAGCAAGATTCGAAATTTGACTTTTTCGACGACCCCACGATGAGTTCTTCATCCAAAACGGCGAGGAAGCTGCAGGTGGCGTCGCCGGTGCCGGCGGACATCGACATTGCCAACTCTGTCGAGCCTCTCCACATCTCTGAGATTGCAAAAGAGCTTCATCTCAGTCCCGATCACTATGATCTTTATGGAAAGTACAAGGCCAAGGTTTTGTTATCAGTGATTGATGAGCTGAAAGGATCTGGAGATGGGTATTATGTGGTGGTTGGAGGGATAACTCCTACCCCTCTTGGAGAAGGCAAGTCGACCACTACTGTTGGACTCTGTCAAGCTTTGGGTGCTTTTCAGGATAAGAAGGTTGTTACTTGCCTTCGTCAACCATCACAAGGACCAACATTTGGAATCAAAGGGGGTGCAGCAGGTGGTGGTTATAGTCAGGTGATCCCAATGGATGAGTTTAATCTTCACATGACAGGAGATATTCATGCAATAACAGCTGCAAACAATCTTCTGGCTGCTGCCATTGATACTCGGATTTTCCACGAGGCAACCCAATCAGATAAGGCTCTACTGAACCGGTTATGTCCACCAAACAAAGAAGGAAAACGGAGTTTCAATGACATCATGTTTAGACGTCTAAAGAAGCTTGGCATCTCCAAGACCACACCCGAGGAGCTTACACTGGAAGAAGTTAAGCAATTTGCTAGGCTTGATATTGACCCAGATTCTATCACTTGGAGAAGAGTAATGGATATAAATGATCGGTTCTTGAGGAAGATTTCAATTGGCCAAGGCCCTGATGAGAAAGGGATGGTAAGAGAAACAGGATTTGATATTTCAGTTGCCAGTGAAATAATGGCAGTTTTGGCCCTTACAACATCTCTAGCGGATATGAGGGAGAGGCTTGGGAAAATGGTGATTGGAAACAGCAGGGCTGGTGACCCTGTAACTGCTGATGATCTTGGTGTTGGAGGTGCATTAACAGTGCTAATGAAGGATGCTATTAACCCTACATTAATGCAGACTCTTGAGGGAACCCCAGTTCTTGTTCATGCTGGTCCTTTTGCAAACATTGCGCACGGGAATTCCTCTATTGTGGCCGATAAGATTGCACTGAAACTGGTAGGACCAGGTGGGTTTGTAGTCACAGAAGCTGGTTTTGGTGCTGATATTGGTACTGAGAAGTTCATGAACATAAAATGCAGATATAGTGGTTTAACACCACAATGTGCAGTTATAGTTGCAACTATAAGGGCCTTGAAAATGCATGGTGGGGGGCCTGATGTTGTTGCTGGGAAGCCTCTTAACAAGGCCTATGTAACTGAGGATGTGGCTTTGGTTGAGGCGGGTTGTGTGAATTTGGCCAAGCATATTTCAAACACAAAAGCCTATGGTGTGAATGTTGTTGTTGCTGTGAACAAGTTCTCGACTGATAGTGAAGCAGAACTAAATGCAGTCAGAAATGCAGCATTGGCTGCTGGGGCATATGATGCTGTTATTTGCACTCATCACGCCCATGGTGGAAAAGGAGCGGTGGACCTAGGAATTGCAGTTCAGAAAGCCTGCGAGAATGTTACACAACCACTGAAGTTTTTATATCCTCTGGAGTTTAGTATTAAAGAGAAAATAGAAGCAATAGCCAACTCATATGGCGCGAGCAGTGTTGAATACTCAGAGCAGGCTGAGAAACAAATTGAGATGTACAGCAAGCAAGGGTTTTCTGTTCTGCCCATATGCATGGCGAAAACCCAGTATTCGTTTTCACATAATGCTTCTGAGAAAGGAGCTCCCACGGGATTCGTCTTACCAATAAGGGATGTAAGAGCTAGCATTGGAGCTGGATTCATCTATCCTTTGGTGGGAACAATGAGTACAATGCCAGGTCTTCCAACTCGTCCTTGCTTCTATGATATTGATCTTGACACCACAACCGGAAGGGTCATCGGTCTTTCTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004329 GO:0004477 GO:0004488 GO:0005488 GO:0005507 GO:0005575 GO:0005576 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005886 GO:0006082 GO:0006139 GO:0006144 GO:0006575 GO:0006725 GO:0006732 GO:0006760 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009108 GO:0009112 GO:0009113 GO:0009256 GO:0009257 GO:0009396 GO:0009507 GO:0009536 GO:0009719 GO:0009725 GO:0009735 GO:0009987 GO:0010033 GO:0010035 GO:0010038 GO:0016020 GO:0016053 GO:0016491 GO:0016645 GO:0016646 GO:0016787 GO:0016810 GO:0016814 GO:0016874 GO:0016879 GO:0018130 GO:0019238 GO:0019438 GO:0019752 GO:0034641 GO:0042221 GO:0042398 GO:0042440 GO:0042558 GO:0042559 GO:0043167 GO:0043169 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0043603 GO:0043604 GO:0043648 GO:0043650 GO:0044237 GO:0044238 GO:0044249 GO:0044271 GO:0044281 GO:0044283 GO:0044424 GO:0044444 GO:0044464 GO:0046112 GO:0046148 GO:0046394 GO:0046483 GO:0046653 GO:0046654 GO:0046686 GO:0046872 GO:0046914 GO:0048046 GO:0050896 GO:0051186 GO:0051188 GO:0055086 GO:0055114 GO:0071704 GO:0071944 GO:0072521 GO:0072522 GO:1901360 GO:1901362 GO:1901564 GO:1901566 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00670 ko00720 ko01100 ko01120 ko01200 map00670 map00720 map01100 map01120 map01200

Pfam Domains

Protein Families

FTHFS

Protein Analysis

684

Amino Acids

73.2

Weight (kDa)

6.74

Isoelectric Point (pI)

35.62

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
FTHFS	PF01268	65 - 683	8.9e-253	Formate--tetrahydrofolate ligase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0005676)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G50480
fragaria_vesca	FvH4_3g44470 FvH4_3g44470 FvH4_3g44470 FvH4_3g44470 FvH4_3g44470 FvH4_3g44470 FvH4_3g44470
malus_domestica	MD03G1012400.v1.1 MD11G1015100.v1.1
prunus_persica	Prupe.6G013000_v2.0.a1 Prupe.6G013000_v2.0.a1 Prupe.6G013000_v2.0.a1
pyrus_communis	pycom03g01100 pycom11g01040
rosa_chinensis	RchiOBHm_Chr5g0081161
rosa_laevigata	RLG00000036914
rosa_multiflora	Rmu_sc0016124.1_g000001 Rmu_ssc0000432.1_g000078
rosa_roxburghii	Rroxscaffold_1G00002300
rosa_rugosa	Rorug05G0473600
rosa_samantha	Rh1DG124300 Rh5AG526200 Rh5BG550500 Rh5CG573400 Rh5DG560200
rosa_wichuraiana	Rw1G009600 Rw5G048890

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	2 cut(s)	163, 506
AatII	GACGTC	1 cut(s)	733
Acc16I	TGCGCA	1 cut(s)	1173
Acc36I	ACCTGC	2 cut(s)	163, 506
AccB1I	GGYRCC	1 cut(s)	187
AccB7I	CCANNNNNTGG	1 cut(s)	494
AccBSI	CCGCTC	1 cut(s)	1628
AccI	GTMKAC	1 cut(s)	401
AccII	CGCG	1 cut(s)	1751
AciI	CCGC	4 cut(s)	194, 983, 1442, 1628
AclWI	GGATC	2 cut(s)	343, 532
AcoI	YGGCCR	3 cut(s)	883, 1194, 1458
AcsI	RAATTY	3 cut(s)	113, 1180, 1453
AcuI	CTGAAG	1 cut(s)	1703
AcyI	GRCGYC	2 cut(s)	179, 730
AdeI	CACNNNGTG	1 cut(s)	1325
AfaI	GTAC	4 cut(s)	293, 1273, 1799, 1969
AfiI	CCNNNNNNNGG	3 cut(s)	482, 494, 1955
AgeI	ACCGGT	1 cut(s)	672
AgsI	TTSAA	6 cut(s)	40, 712, 879, 1357, 1473, 1763
AjnI	CCWGG	2 cut(s)	1225, 1975
AjuI	GAANNNNNNNTTGG	2 cut(s)	1239, 1271
AloI	GAACNNNNNNTCC	4 cut(s)	1122, 1154, 1632, 1664
Alw21I	GWGCWC	1 cut(s)	1885
AlwI	GGATC	2 cut(s)	343, 532
AlwNI	CAGNNNCTG	1 cut(s)	1250
Ama87I	CYCGRG	1 cut(s)	764
AoxI	GGCC	9 cut(s)	297, 883, 889, 963, 1194, 1350, 1373, 1407, 1458
ApeKI	GCWGC	7 cut(s)	169, 512, 593, 611, 614, 1562, 1571
ApoI	RAATTY	3 cut(s)	113, 1180, 1453
AseI	ATTAAT	1 cut(s)	1112
AsiGI	ACCGGT	1 cut(s)	672
Asp700I	GAANNNNTTC	1 cut(s)	1517
AspA2I	CCTAGG	1 cut(s)	1636
AspLEI	GCGC	3 cut(s)	67, 1174, 1751
AspS9I	GGNCC	8 cut(s)	485, 890, 964, 1154, 1223, 1350, 1373, 1633
AsuHPI	GGTGA	3 cut(s)	547, 1024, 1046
AsuII	TTCGAA	1 cut(s)	110
AsuNHI	GCTAGC	1 cut(s)	1922
AvaI	CYCGRG	1 cut(s)	764
AvaII	GGWCC	4 cut(s)	485, 1154, 1223, 1633
AvrII	CCTAGG	1 cut(s)	1636
BalI	TGGCCA	2 cut(s)	885, 1460
BanI	GGYRCC	1 cut(s)	187
BanII	GRGCYC	1 cut(s)	1885
BauI	CACGAG	1 cut(s)	638
BbsI	GAAGAC	1 cut(s)	1973
Bbv12I	GWGCWC	1 cut(s)	1885
BbvI	GCAGC	7 cut(s)	156, 524, 580, 598, 601, 1558, 1574
BccI	CCATC	3 cut(s)	338, 484, 901
BceAI	ACGGC	1 cut(s)	174
BcgI	CGANNNNNNTGC	4 cut(s)	151, 185, 1187, 1221
BciT130I	CCWGG	2 cut(s)	1227, 1977
BfaI	CTAG	4 cut(s)	801, 980, 1637, 1923
BfmI	CTRYAG	1 cut(s)	170
BfuAI	ACCTGC	2 cut(s)	163, 506
BisI	GCNGC	7 cut(s)	170, 513, 594, 612, 615, 1563, 1572
BlnI	CCTAGG	1 cut(s)	1636
BlsI	GCNGC	7 cut(s)	171, 514, 595, 613, 616, 1564, 1573
Bme1390I	CCNGG	2 cut(s)	1227, 1977
Bme18I	GGWCC	4 cut(s)	485, 1154, 1223, 1633
BmeT110I	CYCGRG	1 cut(s)	764
BmgT120I	GGNCC	8 cut(s)	485, 890, 964, 1154, 1223, 1350, 1373, 1633
BmiI	GGNNCC	3 cut(s)	189, 1132, 1374
BmrFI	CCNGG	2 cut(s)	1227, 1977
BmrI	ACTGGG	2 cut(s)	1130, 1840
BmsI	GCATC	3 cut(s)	756, 1084, 1576
BmtI	GCTAGC	1 cut(s)	1926
BmuI	ACTGGG	2 cut(s)	1130, 1840
BpiI	GAAGAC	1 cut(s)	1973
BpmI	CTGGAG	2 cut(s)	360, 1721
Bpu14I	TTCGAA	1 cut(s)	110
BpuEI	CTTGAG	2 cut(s)	886, 1145
BsaBI	GATNNNNATC	1 cut(s)	2010
BsaHI	GRCGYC	2 cut(s)	179, 730
BsaJI	CCNNGG	6 cut(s)	300, 765, 886, 1612, 1636, 1887
BsaWI	WCCGGW	2 cut(s)	672, 2027
Bsc4I	CCNNNNNNNGG	3 cut(s)	482, 494, 1955
Bse118I	RCCGGY	3 cut(s)	184, 190, 672
Bse1I	ACTGG	5 cut(s)	783, 942, 1136, 1221, 1846
Bse3DI	GCAATG	2 cut(s)	204, 1167
Bse8I	GATNNNNATC	1 cut(s)	2010
BseBI	CCWGG	2 cut(s)	1227, 1977
BseDI	CCNNGG	6 cut(s)	300, 765, 886, 1612, 1636, 1887
BseGI	GGATG	6 cut(s)	149, 553, 912, 1099, 1429, 1918
BseJI	GATNNNNATC	1 cut(s)	2010
BseLI	CCNNNNNNNGG	3 cut(s)	482, 494, 1955
BseMI	GCAATG	2 cut(s)	204, 1167
BseMII	CTCAG	8 cut(s)	22, 228, 274, 1266, 1410, 1770, 1782, 1863
BseNI	ACTGG	5 cut(s)	783, 942, 1136, 1221, 1846
BseRI	GAGGAG	1 cut(s)	782
BseXI	GCAGC	7 cut(s)	156, 524, 580, 598, 601, 1558, 1574
BseYI	CCCAGC	2 cut(s)	1388, 1574
BsgI	GTGCAG	2 cut(s)	531, 1347
Bsh1236I	CGCG	1 cut(s)	1751
BshFI	GGCC	9 cut(s)	299, 885, 891, 965, 1196, 1352, 1375, 1409, 1460
BshNI	GGYRCC	1 cut(s)	187
BshTI	ACCGGT	1 cut(s)	672
BsiHKAI	GWGCWC	1 cut(s)	1885
BsiHKCI	CYCGRG	1 cut(s)	764
BsiSI	CCGG	4 cut(s)	185, 191, 673, 2028
BslFI	GGGAC	1 cut(s)	249
BslI	CCNNNNNNNGG	3 cut(s)	482, 494, 1955
BsmFI	GGGAC	1 cut(s)	249
BsnI	GGCC	9 cut(s)	299, 885, 891, 965, 1196, 1352, 1375, 1409, 1460
BsoBI	CYCGRG	1 cut(s)	764
Bsp119I	TTCGAA	1 cut(s)	110
Bsp1286I	GDGCHC	1 cut(s)	1885
Bsp1407I	TGTACA	1 cut(s)	1797
Bsp143I	GATC	8 cut(s)	87, 268, 277, 335, 537, 856, 1054, 2011
Bsp19I	CCATGG	1 cut(s)	1612
BspACI	CCGC	4 cut(s)	194, 983, 1442, 1628
BspANI	GGCC	9 cut(s)	299, 885, 891, 965, 1196, 1352, 1375, 1409, 1460
BspCNI	CTCAG	8 cut(s)	21, 229, 273, 1267, 1411, 1771, 1781, 1864
BspFNI	CGCG	1 cut(s)	1751
BspHI	TCATGA	1 cut(s)	1284
BspLI	GGNNCC	3 cut(s)	189, 1132, 1374
BspMAI	CTGCAG	1 cut(s)	174
BspMI	ACCTGC	2 cut(s)	163, 506
BspOI	GCTAGC	1 cut(s)	1926
BspPI	GGATC	2 cut(s)	343, 532
BspQI	GCTCTTC	1 cut(s)	35
BspT104I	TTCGAA	1 cut(s)	110
BspT107I	GGYRCC	1 cut(s)	187
BsrBI	CCGCTC	1 cut(s)	1628
BsrDI	GCAATG	2 cut(s)	204, 1167
BsrFI	RCCGGY	3 cut(s)	184, 190, 672
BsrGI	TGTACA	1 cut(s)	1797
BsrI	ACTGG	5 cut(s)	783, 942, 1136, 1221, 1846
BssAI	RCCGGY	3 cut(s)	184, 190, 672
BssECI	CCNNGG	6 cut(s)	300, 765, 886, 1612, 1636, 1887
BssMI	GATC	8 cut(s)	87, 268, 277, 335, 537, 856, 1054, 2011
BssNI	GRCGYC	2 cut(s)	179, 730
BssSI	CACGAG	1 cut(s)	638
BssT1I	CCWWGG	4 cut(s)	300, 886, 1612, 1636
Bst2BI	CACGAG	1 cut(s)	638
Bst2UI	CCWGG	2 cut(s)	1227, 1977
Bst4CI	ACNGT	2 cut(s)	412, 1081
Bst6I	CTCTTC	2 cut(s)	35, 832
BstACI	GRCGYC	2 cut(s)	179, 730
BstAPI	GCANNNNNTGC	3 cut(s)	1169, 1571, 1586
BstAUI	TGTACA	1 cut(s)	1797
BstBI	TTCGAA	1 cut(s)	110
BstC8I	GCNNGC	6 cut(s)	192, 1660, 1753, 1776, 1805, 1924
BstDEI	CTNAG	8 cut(s)	8, 237, 260, 1275, 1419, 1768, 1779, 1872
BstDSI	CCRYGG	2 cut(s)	1612, 1887
BstEII	GGTNACC	1 cut(s)	1034
BstF5I	GGATG	6 cut(s)	149, 553, 912, 1099, 1429, 1918
BstFNI	CGCG	1 cut(s)	1751
BstHHI	GCGC	3 cut(s)	67, 1174, 1751
BstKTI	GATC	8 cut(s)	90, 271, 280, 338, 540, 859, 1057, 2014
BstMBI	GATC	8 cut(s)	87, 268, 277, 335, 537, 856, 1054, 2011
BstMWI	GCNNNNNNNGC	8 cut(s)	434, 590, 962, 1169, 1568, 1571, 1577, 1586
BstNI	CCWGG	2 cut(s)	1227, 1977
BstPI	GGTNACC	1 cut(s)	1034
BstSCI	CCNGG	2 cut(s)	1225, 1975
BstSFI	CTRYAG	1 cut(s)	170
BstUI	CGCG	1 cut(s)	1751
BstV1I	GCAGC	7 cut(s)	156, 524, 580, 598, 601, 1558, 1574
BstV2I	GAAGAC	1 cut(s)	1973
BstX2I	RGATCY	1 cut(s)	335
BstXI	CCANNNNNNTGG	1 cut(s)	1834
BstYI	RGATCY	1 cut(s)	335
BsuRI	GGCC	9 cut(s)	299, 885, 891, 965, 1196, 1352, 1375, 1409, 1460
BtgI	CCRYGG	2 cut(s)	1612, 1887
BtsCI	GGATG	6 cut(s)	149, 553, 912, 1099, 1429, 1918
BtsI	GCAGTG	1 cut(s)	1762
BtsIMutI	CAGTG	7 cut(s)	321, 776, 949, 1086, 1208, 1679, 1762
BveI	ACCTGC	2 cut(s)	163, 506
Cac8I	GCNNGC	6 cut(s)	192, 1660, 1753, 1776, 1805, 1924
CaiI	CAGNNNCTG	1 cut(s)	1250
CciI	TCATGA	1 cut(s)	1284
CfoI	GCGC	3 cut(s)	67, 1174, 1751
Cfr10I	RCCGGY	3 cut(s)	184, 190, 672
Cfr13I	GGNCC	8 cut(s)	485, 890, 964, 1154, 1223, 1350, 1373, 1633
CseI	GACGC	1 cut(s)	168
CsiI	ACCWGGT	1 cut(s)	1225
Csp6I	GTAC	4 cut(s)	292, 1272, 1798, 1968
CspAI	ACCGGT	1 cut(s)	672
CspCI	CAANNNNNGTGG	2 cut(s)	394, 429
CviAII	CATG	8 cut(s)	565, 581, 721, 1148, 1285, 1364, 1613, 1834
CviQI	GTAC	4 cut(s)	292, 1272, 1798, 1968
DdeI	CTNAG	8 cut(s)	8, 237, 260, 1275, 1419, 1768, 1779, 1872
DpnI	GATC	8 cut(s)	89, 270, 279, 337, 539, 858, 1056, 2013
DpnII	GATC	8 cut(s)	87, 268, 277, 335, 537, 856, 1054, 2011
DraIII	CACNNNGTG	1 cut(s)	1325
EaeI	YGGCCR	3 cut(s)	883, 1194, 1458
Eam1104I	CTCTTC	2 cut(s)	35, 832
EarI	CTCTTC	2 cut(s)	35, 832
EciI	GGCGGA	1 cut(s)	209
Ecl136II	GAGCTC	1 cut(s)	1883
Eco130I	CCWWGG	4 cut(s)	300, 886, 1612, 1636
Eco147I	AGGCCT	1 cut(s)	1409
Eco24I	GRGCYC	1 cut(s)	1885
Eco47I	GGWCC	4 cut(s)	485, 1154, 1223, 1633
Eco53kI	GAGCTC	1 cut(s)	1883
Eco57I	CTGAAG	1 cut(s)	1703
Eco88I	CYCGRG	1 cut(s)	764
Eco91I	GGTNACC	1 cut(s)	1034
EcoICRI	GAGCTC	1 cut(s)	1883
EcoO109I	RGGNCCY	3 cut(s)	890, 1350, 1373
EcoO65I	GGTNACC	1 cut(s)	1034
EcoRI	GAATTC	1 cut(s)	1180
EcoRII	CCWGG	2 cut(s)	1225, 1975
EcoT14I	CCWWGG	4 cut(s)	300, 886, 1612, 1636
EcoT22I	ATGCAT	2 cut(s)	1365, 1835
EcoT38I	GRGCYC	1 cut(s)	1885
ErhI	CCWWGG	4 cut(s)	300, 886, 1612, 1636
FaeI	CATG	8 cut(s)	568, 584, 724, 1151, 1288, 1367, 1616, 1837
FaqI	GGGAC	1 cut(s)	249
FatI	CATG	8 cut(s)	564, 580, 720, 1147, 1284, 1363, 1612, 1833
FauI	CCCGC	1 cut(s)	1435
FauNDI	CATATG	3 cut(s)	1582, 1744, 1829
FblI	GTMKAC	1 cut(s)	401
Fnu4HI	GCNGC	7 cut(s)	170, 513, 594, 612, 615, 1563, 1572
FokI	GGATG	6 cut(s)	136, 560, 919, 1106, 1436, 1925
FriOI	GRGCYC	1 cut(s)	1885
Fsp4HI	GCNGC	7 cut(s)	170, 513, 594, 612, 615, 1563, 1572
FspBI	CTAG	4 cut(s)	801, 980, 1637, 1923
FspI	TGCGCA	1 cut(s)	1173
GlaI	GCGC	3 cut(s)	66, 1173, 1750
GluI	GCNGC	7 cut(s)	170, 513, 594, 612, 615, 1563, 1572
GsaI	CCCAGC	2 cut(s)	1392, 1578
GsuI	CTGGAG	2 cut(s)	360, 1721
HaeIII	GGCC	9 cut(s)	299, 885, 891, 965, 1196, 1352, 1375, 1409, 1460
HapII	CCGG	4 cut(s)	185, 191, 673, 2028
HgaI	GACGC	1 cut(s)	168
HhaI	GCGC	3 cut(s)	67, 1174, 1751
Hin1I	GRCGYC	2 cut(s)	179, 730
Hin1II	CATG	8 cut(s)	568, 584, 724, 1151, 1288, 1367, 1616, 1837
Hin6I	GCGC	3 cut(s)	65, 1172, 1749
HinP1I	GCGC	3 cut(s)	65, 1172, 1749
HincII	GTYRAC	2 cut(s)	402, 473
HindII	GTYRAC	2 cut(s)	402, 473
HindIII	AAGCTT	2 cut(s)	426, 740
HinfI	GANTC	7 cut(s)	107, 417, 498, 821, 1120, 1893, 1938
HpaII	CCGG	4 cut(s)	185, 191, 673, 2028
HphI	GGTGA	3 cut(s)	547, 1024, 1046
Hpy166II	GTNNAC	5 cut(s)	402, 473, 683, 1513, 1633
Hpy188I	TCNGA	9 cut(s)	11, 87, 238, 630, 655, 1556, 1653, 1771, 1873
Hpy8I	GTNNAC	5 cut(s)	402, 473, 683, 1513, 1633
Hpy99I	CGWCG	2 cut(s)	131, 184
HpyAV	CCTTC	6 cut(s)	386, 445, 476, 689, 1084, 2025
HpyCH4III	ACNGT	2 cut(s)	412, 1081
HpyCH4IV	ACGT	1 cut(s)	730
HpyF10VI	GCNNNNNNNGC	8 cut(s)	434, 590, 962, 1169, 1568, 1571, 1577, 1586
HpyF3I	CTNAG	8 cut(s)	8, 237, 260, 1275, 1419, 1768, 1779, 1872
HpySE526I	ACGT	1 cut(s)	730
Hsp92I	GRCGYC	2 cut(s)	179, 730
Hsp92II	CATG	8 cut(s)	568, 584, 724, 1151, 1288, 1367, 1616, 1837
HspAI	GCGC	3 cut(s)	65, 1172, 1749
KroI	GCCGGC	1 cut(s)	190
KroNI	GCCGGC	1 cut(s)	192
Kzo9I	GATC	8 cut(s)	87, 268, 277, 335, 537, 856, 1054, 2011
LguI	GCTCTTC	1 cut(s)	35
LmnI	GCTCC	6 cut(s)	9, 769, 1625, 1880, 1888, 1931
Lsp1109I	GCAGC	7 cut(s)	156, 524, 580, 598, 601, 1558, 1574
LweI	GCATC	3 cut(s)	756, 1084, 1576
MabI	ACCWGGT	1 cut(s)	1225
MaeI	CTAG	4 cut(s)	801, 980, 1637, 1923
MaeII	ACGT	1 cut(s)	730
MaeIII	GTNAC	6 cut(s)	457, 1034, 1042, 1240, 1414, 1669
MalI	GATC	8 cut(s)	89, 270, 279, 337, 539, 858, 1056, 2013
MbiI	CCGCTC	1 cut(s)	1628
MboI	GATC	8 cut(s)	87, 268, 277, 335, 537, 856, 1054, 2011
MboII	GAAGA	9 cut(s)	22, 28, 138, 551, 596, 794, 849, 883, 1973
MfeI	CAATTG	1 cut(s)	879
MflI	RGATCY	1 cut(s)	335
MhlI	GDGCHC	1 cut(s)	1885
MlsI	TGGCCA	2 cut(s)	885, 1460
MluCI	AATT	7 cut(s)	113, 794, 879, 1180, 1453, 1641, 1788
MluNI	TGGCCA	2 cut(s)	885, 1460
MlyI	GAGTC	2 cut(s)	411, 1114
MmeI	TCCRAC	4 cut(s)	343, 394, 1045, 2009
Mox20I	TGGCCA	2 cut(s)	885, 1460
Mph1103I	ATGCAT	2 cut(s)	1365, 1835
MroNI	GCCGGC	1 cut(s)	190
MroXI	GAANNNNTTC	1 cut(s)	1517
MscI	TGGCCA	2 cut(s)	885, 1460
MseI	TTAA	8 cut(s)	555, 789, 1076, 1101, 1112, 1313, 1401, 1713
MslI	CAYNNNNRTG	3 cut(s)	1321, 1611, 1832
Msp20I	TGGCCA	2 cut(s)	885, 1460
MspA1I	CMGCKG	1 cut(s)	593
MspI	CCGG	4 cut(s)	185, 191, 673, 2028
MspR9I	CCNGG	2 cut(s)	1227, 1977
MunI	CAATTG	1 cut(s)	879
MvaI	CCWGG	2 cut(s)	1227, 1977
MvnI	CGCG	1 cut(s)	1751
MwoI	GCNNNNNNNGC	8 cut(s)	434, 590, 962, 1169, 1568, 1571, 1577, 1586
NaeI	GCCGGC	1 cut(s)	192
NcoI	CCATGG	1 cut(s)	1612
NdeI	CATATG	3 cut(s)	1582, 1744, 1829
NdeII	GATC	8 cut(s)	87, 268, 277, 335, 537, 856, 1054, 2011
NgoMIV	GCCGGC	1 cut(s)	190
NheI	GCTAGC	1 cut(s)	1922
NlaIII	CATG	8 cut(s)	568, 584, 724, 1151, 1288, 1367, 1616, 1837
NlaIV	GGNNCC	3 cut(s)	189, 1132, 1374
NmuCI	GTSAC	2 cut(s)	1034, 1240
NsbI	TGCGCA	1 cut(s)	1173
NsiI	ATGCAT	2 cut(s)	1365, 1835
NspV	TTCGAA	1 cut(s)	110
PagI	TCATGA	1 cut(s)	1284
PaqCI	CACCTGC	2 cut(s)	163, 506
PceI	AGGCCT	1 cut(s)	1409
PciSI	GCTCTTC	1 cut(s)	35
PdiI	GCCGGC	1 cut(s)	192
PdmI	GAANNNNTTC	1 cut(s)	1517
PfeI	GAWTC	5 cut(s)	107, 498, 821, 1893, 1938
PflFI	GACNNNGTC	1 cut(s)	420
PflMI	CCANNNNNTGG	1 cut(s)	494
PinAI	ACCGGT	1 cut(s)	672
PkrI	GCNGC	7 cut(s)	171, 514, 595, 613, 616, 1564, 1573
PleI	GAGTC	2 cut(s)	411, 1114
PpsI	GAGTC	2 cut(s)	411, 1114
PshBI	ATTAAT	1 cut(s)	1112
Psp124BI	GAGCTC	1 cut(s)	1885
Psp6I	CCWGG	2 cut(s)	1225, 1975
PspEI	GGTNACC	1 cut(s)	1034
PspFI	CCCAGC	2 cut(s)	1388, 1574
PspGI	CCWGG	2 cut(s)	1225, 1975
PspN4I	GGNNCC	3 cut(s)	189, 1132, 1374
PspPI	GGNCC	8 cut(s)	485, 890, 964, 1154, 1223, 1350, 1373, 1633
PsrI	GAACNNNNNNTAC	2 cut(s)	1951, 1983
PstI	CTGCAG	1 cut(s)	174
PstNI	CAGNNNCTG	1 cut(s)	1250
PsuI	RGATCY	1 cut(s)	335
PsyI	GACNNNGTC	1 cut(s)	420
PvuII	CAGCTG	1 cut(s)	593
RsaI	GTAC	4 cut(s)	293, 1273, 1799, 1969
RsaNI	GTAC	4 cut(s)	292, 1272, 1798, 1968
RseI	CAYNNNNRTG	3 cut(s)	1321, 1611, 1832
SacI	GAGCTC	1 cut(s)	1885
SalI	GTCGAC	1 cut(s)	400
SapI	GCTCTTC	1 cut(s)	35
SaqAI	TTAA	8 cut(s)	555, 789, 1076, 1101, 1112, 1313, 1401, 1713
SatI	GCNGC	7 cut(s)	170, 513, 594, 612, 615, 1563, 1572
Sau3AI	GATC	8 cut(s)	87, 268, 277, 335, 537, 856, 1054, 2011
Sau96I	GGNCC	8 cut(s)	485, 890, 964, 1154, 1223, 1350, 1373, 1633
SchI	GAGTC	2 cut(s)	411, 1114
ScrFI	CCNGG	2 cut(s)	1227, 1977
SduI	GDGCHC	1 cut(s)	1885
SexAI	ACCWGGT	1 cut(s)	1225
SfaNI	GCATC	3 cut(s)	756, 1084, 1576
SfcI	CTRYAG	1 cut(s)	170
SfuI	TTCGAA	1 cut(s)	110
SgrAI	CRCCGGYG	1 cut(s)	184
SinI	GGWCC	4 cut(s)	485, 1154, 1223, 1633
SmiMI	CAYNNNNRTG	3 cut(s)	1321, 1611, 1832
SmlI	CTYRAG	2 cut(s)	865, 1124
SmoI	CTYRAG	2 cut(s)	865, 1124
Sse9I	AATT	7 cut(s)	113, 794, 879, 1180, 1453, 1641, 1788
SseBI	AGGCCT	1 cut(s)	1409
SsiI	CCGC	4 cut(s)	194, 983, 1442, 1628
SspMI	CTAG	4 cut(s)	801, 980, 1637, 1923
SstI	GAGCTC	1 cut(s)	1885
StuI	AGGCCT	1 cut(s)	1409
StyD4I	CCNGG	2 cut(s)	1225, 1975
StyI	CCWWGG	4 cut(s)	300, 886, 1612, 1636
TaaI	ACNGT	2 cut(s)	412, 1081
TaiI	ACGT	1 cut(s)	733
TaqI	TCGA	6 cut(s)	110, 126, 201, 219, 401, 1523
TaqII	GACCGA	1 cut(s)	2030
TasI	AATT	7 cut(s)	113, 794, 879, 1180, 1453, 1641, 1788
TatI	WGTACW	3 cut(s)	291, 1797, 1967
TfiI	GAWTC	5 cut(s)	107, 498, 821, 1893, 1938
Tru1I	TTAA	8 cut(s)	555, 789, 1076, 1101, 1112, 1313, 1401, 1713
Tru9I	TTAA	8 cut(s)	555, 789, 1076, 1101, 1112, 1313, 1401, 1713
TscAI	CASTG	7 cut(s)	321, 783, 949, 1086, 1215, 1686, 1762
TseFI	GTSAC	2 cut(s)	1034, 1240
TseI	GCWGC	7 cut(s)	169, 512, 593, 611, 614, 1562, 1571
Tsp45I	GTSAC	2 cut(s)	1034, 1240
TspDTI	ATGAA	9 cut(s)	22, 138, 245, 569, 1103, 1136, 1273, 1301, 1930
TspGWI	ACGGA	1 cut(s)	718
TspRI	CASTG	7 cut(s)	321, 783, 949, 1086, 1215, 1686, 1762
Tth111I	GACNNNGTC	1 cut(s)	420
Van91I	CCANNNNNTGG	1 cut(s)	494
VpaK11BI	GGWCC	4 cut(s)	485, 1154, 1223, 1633
VspI	ATTAAT	1 cut(s)	1112
XapI	RAATTY	3 cut(s)	113, 1180, 1453
XcmI	CCANNNNNNNNNTGG	1 cut(s)	949
XmaJI	CCTAGG	1 cut(s)	1636
XmiI	GTMKAC	1 cut(s)	401
XmnI	GAANNNNTTC	1 cut(s)	1517
XspI	CTAG	4 cut(s)	801, 980, 1637, 1923
ZraI	GACGTC	1 cut(s)	731
Zsp2I	ATGCAT	2 cut(s)	1365, 1835

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.