Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr11

Orthogroup ID

OG0005676

Physical Location & Seq

Genomic Coordinates Forward (+)

1197425 .. 1201770

Gene Structure 4346 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD11G1015100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1914 bp

ATGAGTCCTTCATCCAAGACGGCGAGGAAGCTGCAGGTGGCGTCGCCAGTGCCGGCGGATATCGACATTGCCAACTCTGTCGAGCCTTTCCACATCTCTGAGATTGCAAAAGAGCTTAATCTCAGTCCCGACCACTACGATCTTTATGGGAAATACAAAGCCAAGGTTTTGTTATCAGTGATTGATGAGCTGAAAGGATCTGGAGATGGGTATTATGTGGTGGTTGGAGGGATTACTCCTACCCCTCTTGGAGAAGGCAAGTCTACCACTACTGTTGGACTCTGTCAAGCTTTGGGTGCTTTTCAGGATAAGAAGGTTGTTACTTGCCTTCGTCAGCCATCACAAGGACCAACTTTTGGAATCAAAGGGGGTGCAGCAGGTGGTGGTTACAGTCAGGTGATACCAATGGATGAGTTTAATCTTCACATGACGGGAGATATTCATGCAATAACAGCTGCAAACAATCTTCTGGCTGCTGCCATTGATACTCGGATTTTCCACGAGGCAACCCAATCAGATAAGGCTCTACTGAACCGGTTATGTCCACCAAACAAAGAAGGAAAACGGAGCTTCAATGACATCATGTTTAGACGTCTAAAGAAGCTGAGTATCTCCAAGACCACGCCCGAGGAGCTTACACCGGAAGAAGTTAAGCAATTTGCCAGGCTTGATATTGACCCAAATTCTATCACATGGAGAAGAGTAATGGATATAAATGACCGGTTCTTGAGGAAGATTTCAATTGGTCAAGGCCCTGACGAGAAAGGGATGGTAAGAGAAACAGGATTTGATATTTCAGTTGCCAGTGAAATAATGGCAGTTTTGGCCCTTACAACATCTCTAACGGATATGAGGGAGAGGCTTGGGAAAATGGTGATTGGAAACAGCAGGGCTGGTGACCCTGTAACCGCCGATGATCTTGGTGTTGGAGGTGCTTTAACAGTGCTAATGAAGGATGCTATTAACCCTACATTAATGCAGACTCTTGAGGGAACCCCAGTTCTTGTTCATGCTGGTCCTTTTGCAAACATTGCGCACGGGAATTCCTCCATTGTGGCTGATAAGATTGCACTGAAACTGGTAGGACCAGGTGGGTTTGTGGTCACAGAAGCTGGTTTTGGTGCTGATATTGGTACTGAAAAGTTCATGAACATAAAATGCAGGTATAGTGGTTTAACACCACAATGTGCAGTTATAGTTGCAACTATAAGGGCCTTGAAAATGCATGGTGGCGGGCCTGATGTTGTTGCCGGGAAGCCTCTTAACAAGGCCTACGTTACTGAGGATGTTGCTTTGGTTGAGGCGGGTTGTGTGAATTTGGCCAAGCATATTTCAAACACAAAAGCCTATGGTGTGAATGTTGTTGTTGCCGTGAACAAGTTCTCGACCGATAGTGAAGCAGAACTAAATGCAGTCAGAAATGCAGCATTGGCTGCTGGGGCATATGATGCCGTTATTTGTACTCATCACGCCCATGGTGGAAAAGGAGCGGTAGACCTAGGAATTGCGGTTCAAAAAGCCTGTGAGAATGTTACACAACCTTTGAAGTTCTTATATCCTCTAGAGTTTAGTATTAAAGAGAAAATAGAAGCAATAGCCAAGTCATATGGAGCGAGCGGTGTTGAATACTCAGAGCAGGCTGAGAAACAAATTGAGATGTACAGCAAGCAAGGGTTTTCTGGTCTGCCCATATGCATGGCGAAAACCCAGTATTCGTTTTCACATAATGCTTCTGAGAAAGGAGCCCCCACCGGATTCGTCTTACCAATAAGGGATGTAAGAGCTAGCATTGGAGCTGGATTCATCTATCCTTTGGTGGGAACAATGAGTACAATGCCGGGTCTTCCAACTCGCCCTTGCTTCTATGACATTGATCTTGACACTGCAACCGGAAGGGTCATCGGTCTTTCTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004329 GO:0004477 GO:0004488 GO:0005488 GO:0005507 GO:0005575 GO:0005576 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005886 GO:0006082 GO:0006139 GO:0006144 GO:0006575 GO:0006725 GO:0006732 GO:0006760 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009108 GO:0009112 GO:0009113 GO:0009256 GO:0009257 GO:0009396 GO:0009507 GO:0009536 GO:0009719 GO:0009725 GO:0009735 GO:0009987 GO:0010033 GO:0010035 GO:0010038 GO:0016020 GO:0016053 GO:0016491 GO:0016645 GO:0016646 GO:0016787 GO:0016810 GO:0016814 GO:0016874 GO:0016879 GO:0018130 GO:0019238 GO:0019438 GO:0019752 GO:0034641 GO:0042221 GO:0042398 GO:0042440 GO:0042558 GO:0042559 GO:0043167 GO:0043169 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0043603 GO:0043604 GO:0043648 GO:0043650 GO:0044237 GO:0044238 GO:0044249 GO:0044271 GO:0044281 GO:0044283 GO:0044424 GO:0044444 GO:0044464 GO:0046112 GO:0046148 GO:0046394 GO:0046483 GO:0046653 GO:0046654 GO:0046686 GO:0046872 GO:0046914 GO:0048046 GO:0050896 GO:0051186 GO:0051188 GO:0055086 GO:0055114 GO:0071704 GO:0071944 GO:0072521 GO:0072522 GO:1901360 GO:1901362 GO:1901564 GO:1901566 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00670 ko00720 ko01100 ko01120 ko01200 map00670 map00720 map01100 map01120 map01200

Pfam Domains

Protein Families

FTHFS

Protein Analysis

638

Amino Acids

67.78

Weight (kDa)

6.9

Isoelectric Point (pI)

35.35

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
FTHFS	PF01268	19 - 637	2.5e-253	Formate--tetrahydrofolate ligase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0005676)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G50480
fragaria_vesca	FvH4_3g44470 FvH4_3g44470 FvH4_3g44470 FvH4_3g44470 FvH4_3g44470 FvH4_3g44470 FvH4_3g44470
malus_domestica	MD03G1012400.v1.1 MD11G1015100.v1.1
prunus_persica	Prupe.6G013000_v2.0.a1 Prupe.6G013000_v2.0.a1 Prupe.6G013000_v2.0.a1
pyrus_communis	pycom03g01100 pycom11g01040
rosa_chinensis	RchiOBHm_Chr5g0081161
rosa_laevigata	RLG00000036914
rosa_multiflora	Rmu_sc0016124.1_g000001 Rmu_ssc0000432.1_g000078
rosa_roxburghii	Rroxscaffold_1G00002300
rosa_rugosa	Rorug05G0473600
rosa_samantha	Rh1DG124300 Rh5AG526200 Rh5BG550500 Rh5CG573400 Rh5DG560200
rosa_wichuraiana	Rw1G009600 Rw5G048890

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	2 cut(s)	25, 368
AatII	GACGTC	1 cut(s)	595
Acc16I	TGCGCA	1 cut(s)	1035
Acc36I	ACCTGC	3 cut(s)	25, 368, 1152
AccB7I	CCANNNNNTGG	1 cut(s)	356
AccBSI	CCGCTC	2 cut(s)	1490, 1617
AccI	GTMKAC	2 cut(s)	263, 1494
AciI	CCGC	7 cut(s)	56, 909, 1233, 1304, 1490, 1508, 1617
AclWI	GGATC	1 cut(s)	205
AcoI	YGGCCR	1 cut(s)	1320
AcsI	RAATTY	3 cut(s)	682, 1042, 1315
AcyI	GRCGYC	2 cut(s)	41, 592
AdeI	CACNNNGTG	1 cut(s)	1187
AfaI	GTAC	4 cut(s)	1135, 1462, 1661, 1831
AfiI	CCNNNNNNNGG	3 cut(s)	344, 356, 1817
AgeI	ACCGGT	2 cut(s)	534, 720
AgsI	TTSAA	7 cut(s)	574, 741, 1219, 1335, 1514, 1546, 1625
AjnI	CCWGG	2 cut(s)	662, 1087
AjuI	GAANNNNNNNTTGG	2 cut(s)	1101, 1133
AloI	GAACNNNNNNTCC	4 cut(s)	984, 1016, 1494, 1526
AlwI	GGATC	1 cut(s)	205
AlwNI	CAGNNNCTG	1 cut(s)	1112
Ama87I	CYCGRG	1 cut(s)	626
AoxI	GGCC	6 cut(s)	751, 825, 1212, 1235, 1269, 1320
ApeKI	GCWGC	7 cut(s)	31, 374, 455, 473, 476, 1424, 1433
ApoI	RAATTY	3 cut(s)	682, 1042, 1315
AseI	ATTAAT	1 cut(s)	974
AsiGI	ACCGGT	2 cut(s)	534, 720
Asp700I	GAANNNNTTC	1 cut(s)	1379
AspA2I	CCTAGG	1 cut(s)	1498
AspLEI	GCGC	1 cut(s)	1036
AspS9I	GGNCC	7 cut(s)	347, 752, 826, 1016, 1085, 1212, 1235
AsuC2I	CCSGG	2 cut(s)	1252, 1839
AsuHPI	GGTGA	3 cut(s)	409, 886, 908
AsuNHI	GCTAGC	1 cut(s)	1784
AvaI	CYCGRG	1 cut(s)	626
AvaII	GGWCC	3 cut(s)	347, 1016, 1085
AvrII	CCTAGG	1 cut(s)	1498
BalI	TGGCCA	1 cut(s)	1322
BanII	GRGCYC	1 cut(s)	1747
BauI	CACGAG	1 cut(s)	500
BbsI	GAAGAC	1 cut(s)	1835
BbvI	GCAGC	7 cut(s)	18, 386, 442, 460, 463, 1420, 1436
BccI	CCATC	3 cut(s)	200, 346, 763
BceAI	ACGGC	3 cut(s)	36, 1355, 1436
BcgI	CGANNNNNNTGC	2 cut(s)	13, 47
BciT130I	CCWGG	2 cut(s)	664, 1089
BcnI	CCSGG	2 cut(s)	1252, 1839
BfaI	CTAG	3 cut(s)	1499, 1562, 1785
BfmI	CTRYAG	1 cut(s)	32
BfuAI	ACCTGC	3 cut(s)	25, 368, 1152
BisI	GCNGC	7 cut(s)	32, 375, 456, 474, 477, 1425, 1434
BlnI	CCTAGG	1 cut(s)	1498
BlsI	GCNGC	7 cut(s)	33, 376, 457, 475, 478, 1426, 1435
Bme1390I	CCNGG	4 cut(s)	664, 1089, 1252, 1839
Bme18I	GGWCC	3 cut(s)	347, 1016, 1085
BmeT110I	CYCGRG	1 cut(s)	626
BmgT120I	GGNCC	7 cut(s)	347, 752, 826, 1016, 1085, 1212, 1235
BmiI	GGNNCC	2 cut(s)	994, 1744
BmrFI	CCNGG	4 cut(s)	664, 1089, 1252, 1839
BmrI	ACTGGG	2 cut(s)	992, 1702
BmsI	GCATC	2 cut(s)	946, 1438
BmtI	GCTAGC	1 cut(s)	1788
BmuI	ACTGGG	2 cut(s)	992, 1702
BpiI	GAAGAC	1 cut(s)	1835
BpmI	CTGGAG	1 cut(s)	222
BpuEI	CTTGAG	2 cut(s)	748, 1007
BpuMI	CCSGG	2 cut(s)	1252, 1839
BsaHI	GRCGYC	2 cut(s)	41, 592
BsaJI	CCNNGG	4 cut(s)	162, 627, 1474, 1498
BsaWI	WCCGGW	5 cut(s)	534, 640, 720, 1751, 1889
Bsc4I	CCNNNNNNNGG	3 cut(s)	344, 356, 1817
Bse118I	RCCGGY	3 cut(s)	52, 534, 720
Bse1I	ACTGG	5 cut(s)	47, 804, 998, 1083, 1708
Bse3DI	GCAATG	2 cut(s)	66, 1029
BseBI	CCWGG	2 cut(s)	664, 1089
BseDI	CCNNGG	4 cut(s)	162, 627, 1474, 1498
BseGI	GGATG	6 cut(s)	11, 415, 774, 961, 1291, 1780
BseLI	CCNNNNNNNGG	3 cut(s)	344, 356, 1817
BseMI	GCAATG	2 cut(s)	66, 1029
BseMII	CTCAG	7 cut(s)	90, 136, 596, 1272, 1632, 1644, 1725
BseNI	ACTGG	5 cut(s)	47, 804, 998, 1083, 1708
BseRI	GAGGAG	1 cut(s)	644
BseXI	GCAGC	7 cut(s)	18, 386, 442, 460, 463, 1420, 1436
BseYI	CCCAGC	1 cut(s)	1436
BsgI	GTGCAG	2 cut(s)	393, 1209
Bsh1285I	CGRYCG	1 cut(s)	1389
BshFI	GGCC	6 cut(s)	753, 827, 1214, 1237, 1271, 1322
BshTI	ACCGGT	2 cut(s)	534, 720
BsiEI	CGRYCG	1 cut(s)	1389
BsiHKCI	CYCGRG	1 cut(s)	626
BsiSI	CCGG	8 cut(s)	53, 535, 641, 721, 1251, 1752, 1838, 1890
BslFI	GGGAC	1 cut(s)	111
BslI	CCNNNNNNNGG	3 cut(s)	344, 356, 1817
BsmFI	GGGAC	1 cut(s)	111
BsnI	GGCC	6 cut(s)	753, 827, 1214, 1237, 1271, 1322
BsoBI	CYCGRG	1 cut(s)	626
Bsp1286I	GDGCHC	1 cut(s)	1747
Bsp1407I	TGTACA	1 cut(s)	1659
Bsp143I	GATC	4 cut(s)	139, 197, 916, 1873
Bsp19I	CCATGG	1 cut(s)	1474
BspACI	CCGC	7 cut(s)	56, 909, 1233, 1304, 1490, 1508, 1617
BspANI	GGCC	6 cut(s)	753, 827, 1214, 1237, 1271, 1322
BspCNI	CTCAG	7 cut(s)	91, 135, 597, 1273, 1633, 1643, 1726
BspHI	TCATGA	1 cut(s)	1146
BspLI	GGNNCC	2 cut(s)	994, 1744
BspMAI	CTGCAG	1 cut(s)	36
BspMI	ACCTGC	3 cut(s)	25, 368, 1152
BspOI	GCTAGC	1 cut(s)	1788
BspPI	GGATC	1 cut(s)	205
BsrBI	CCGCTC	2 cut(s)	1490, 1617
BsrDI	GCAATG	2 cut(s)	66, 1029
BsrFI	RCCGGY	3 cut(s)	52, 534, 720
BsrGI	TGTACA	1 cut(s)	1659
BsrI	ACTGG	5 cut(s)	47, 804, 998, 1083, 1708
BssAI	RCCGGY	3 cut(s)	52, 534, 720
BssECI	CCNNGG	4 cut(s)	162, 627, 1474, 1498
BssMI	GATC	4 cut(s)	139, 197, 916, 1873
BssNI	GRCGYC	2 cut(s)	41, 592
BssSI	CACGAG	1 cut(s)	500
BssT1I	CCWWGG	3 cut(s)	162, 1474, 1498
Bst2BI	CACGAG	1 cut(s)	500
Bst2UI	CCWGG	2 cut(s)	664, 1089
Bst4CI	ACNGT	3 cut(s)	274, 392, 943
Bst6I	CTCTTC	1 cut(s)	694
BstACI	GRCGYC	2 cut(s)	41, 592
BstAPI	GCANNNNNTGC	3 cut(s)	1031, 1433, 1448
BstAUI	TGTACA	1 cut(s)	1659
BstC8I	GCNNGC	6 cut(s)	54, 1235, 1615, 1638, 1667, 1786
BstDEI	CTNAG	7 cut(s)	99, 122, 605, 1281, 1630, 1641, 1734
BstDSI	CCRYGG	1 cut(s)	1474
BstEII	GGTNACC	1 cut(s)	896
BstF5I	GGATG	6 cut(s)	11, 415, 774, 961, 1291, 1780
BstHHI	GCGC	1 cut(s)	1036
BstKTI	GATC	4 cut(s)	142, 200, 919, 1876
BstMBI	GATC	4 cut(s)	139, 197, 916, 1873
BstMCI	CGRYCG	1 cut(s)	1389
BstMWI	GCNNNNNNNGC	9 cut(s)	296, 452, 631, 824, 1031, 1430, 1433, 1439, 1448
BstNI	CCWGG	2 cut(s)	664, 1089
BstPI	GGTNACC	1 cut(s)	896
BstSCI	CCNGG	4 cut(s)	662, 1087, 1250, 1837
BstSFI	CTRYAG	1 cut(s)	32
BstV1I	GCAGC	7 cut(s)	18, 386, 442, 460, 463, 1420, 1436
BstV2I	GAAGAC	1 cut(s)	1835
BstX2I	RGATCY	1 cut(s)	197
BstXI	CCANNNNNNTGG	1 cut(s)	1696
BstYI	RGATCY	1 cut(s)	197
BsuRI	GGCC	6 cut(s)	753, 827, 1214, 1237, 1271, 1322
BtgI	CCRYGG	1 cut(s)	1474
BtsCI	GGATG	6 cut(s)	11, 415, 774, 961, 1291, 1780
BtsI	GCAGTG	1 cut(s)	1881
BtsIMutI	CAGTG	6 cut(s)	54, 183, 811, 948, 1070, 1881
BveI	ACCTGC	3 cut(s)	25, 368, 1152
Cac8I	GCNNGC	6 cut(s)	54, 1235, 1615, 1638, 1667, 1786
CaiI	CAGNNNCTG	1 cut(s)	1112
CciI	TCATGA	1 cut(s)	1146
CfoI	GCGC	1 cut(s)	1036
Cfr10I	RCCGGY	3 cut(s)	52, 534, 720
Cfr13I	GGNCC	7 cut(s)	347, 752, 826, 1016, 1085, 1212, 1235
CseI	GACGC	1 cut(s)	30
CsiI	ACCWGGT	1 cut(s)	1087
Csp6I	GTAC	4 cut(s)	1134, 1461, 1660, 1830
CspAI	ACCGGT	2 cut(s)	534, 720
CspCI	CAANNNNNGTGG	2 cut(s)	256, 291
CviAII	CATG	9 cut(s)	427, 443, 583, 693, 1010, 1147, 1226, 1475, 1696
CviQI	GTAC	4 cut(s)	1134, 1461, 1660, 1830
DdeI	CTNAG	7 cut(s)	99, 122, 605, 1281, 1630, 1641, 1734
DpnI	GATC	4 cut(s)	141, 199, 918, 1875
DpnII	GATC	4 cut(s)	139, 197, 916, 1873
DraIII	CACNNNGTG	1 cut(s)	1187
EaeI	YGGCCR	1 cut(s)	1320
Eam1104I	CTCTTC	1 cut(s)	694
EarI	CTCTTC	1 cut(s)	694
EciI	GGCGGA	1 cut(s)	71
Eco130I	CCWWGG	3 cut(s)	162, 1474, 1498
Eco147I	AGGCCT	1 cut(s)	1271
Eco24I	GRGCYC	1 cut(s)	1747
Eco32I	GATATC	1 cut(s)	61
Eco47I	GGWCC	3 cut(s)	347, 1016, 1085
Eco88I	CYCGRG	1 cut(s)	626
Eco91I	GGTNACC	1 cut(s)	896
EcoO109I	RGGNCCY	2 cut(s)	752, 1212
EcoO65I	GGTNACC	1 cut(s)	896
EcoRI	GAATTC	1 cut(s)	1042
EcoRII	CCWGG	2 cut(s)	662, 1087
EcoRV	GATATC	1 cut(s)	61
EcoT14I	CCWWGG	3 cut(s)	162, 1474, 1498
EcoT22I	ATGCAT	2 cut(s)	1227, 1697
EcoT38I	GRGCYC	1 cut(s)	1747
ErhI	CCWWGG	3 cut(s)	162, 1474, 1498
FaeI	CATG	9 cut(s)	430, 446, 586, 696, 1013, 1150, 1229, 1478, 1699
FaqI	GGGAC	1 cut(s)	111
FatI	CATG	9 cut(s)	426, 442, 582, 692, 1009, 1146, 1225, 1474, 1695
FauI	CCCGC	2 cut(s)	1226, 1297
FauNDI	CATATG	3 cut(s)	1444, 1606, 1691
FblI	GTMKAC	2 cut(s)	263, 1494
Fnu4HI	GCNGC	7 cut(s)	32, 375, 456, 474, 477, 1425, 1434
FokI	GGATG	5 cut(s)	422, 781, 968, 1298, 1787
FriOI	GRGCYC	1 cut(s)	1747
Fsp4HI	GCNGC	7 cut(s)	32, 375, 456, 474, 477, 1425, 1434
FspBI	CTAG	3 cut(s)	1499, 1562, 1785
FspI	TGCGCA	1 cut(s)	1035
GlaI	GCGC	1 cut(s)	1035
GluI	GCNGC	7 cut(s)	32, 375, 456, 474, 477, 1425, 1434
GsaI	CCCAGC	1 cut(s)	1440
GsuI	CTGGAG	1 cut(s)	222
HaeIII	GGCC	6 cut(s)	753, 827, 1214, 1237, 1271, 1322
HapII	CCGG	8 cut(s)	53, 535, 641, 721, 1251, 1752, 1838, 1890
HgaI	GACGC	1 cut(s)	30
HhaI	GCGC	1 cut(s)	1036
Hin1I	GRCGYC	2 cut(s)	41, 592
Hin1II	CATG	9 cut(s)	430, 446, 586, 696, 1013, 1150, 1229, 1478, 1699
Hin6I	GCGC	1 cut(s)	1034
HinP1I	GCGC	1 cut(s)	1034
HindIII	AAGCTT	1 cut(s)	288
HinfI	GANTC	6 cut(s)	4, 279, 360, 982, 1755, 1800
HpaII	CCGG	8 cut(s)	53, 535, 641, 721, 1251, 1752, 1838, 1890
HphI	GGTGA	3 cut(s)	409, 886, 908
Hpy166II	GTNNAC	4 cut(s)	264, 545, 1375, 1495
Hpy188I	TCNGA	6 cut(s)	100, 492, 517, 1418, 1633, 1735
Hpy8I	GTNNAC	4 cut(s)	264, 545, 1375, 1495
Hpy99I	CGWCG	1 cut(s)	46
HpyAV	CCTTC	7 cut(s)	18, 248, 307, 338, 551, 946, 1887
HpyCH4III	ACNGT	3 cut(s)	274, 392, 943
HpyCH4IV	ACGT	2 cut(s)	592, 1275
HpyF10VI	GCNNNNNNNGC	9 cut(s)	296, 452, 631, 824, 1031, 1430, 1433, 1439, 1448
HpyF3I	CTNAG	7 cut(s)	99, 122, 605, 1281, 1630, 1641, 1734
HpySE526I	ACGT	2 cut(s)	592, 1275
Hsp92I	GRCGYC	2 cut(s)	41, 592
Hsp92II	CATG	9 cut(s)	430, 446, 586, 696, 1013, 1150, 1229, 1478, 1699
HspAI	GCGC	1 cut(s)	1034
KroI	GCCGGC	1 cut(s)	52
KroNI	GCCGGC	1 cut(s)	54
Kzo9I	GATC	4 cut(s)	139, 197, 916, 1873
LmnI	GCTCC	6 cut(s)	567, 631, 1487, 1610, 1742, 1793
Lsp1109I	GCAGC	7 cut(s)	18, 386, 442, 460, 463, 1420, 1436
LweI	GCATC	2 cut(s)	946, 1438
MabI	ACCWGGT	1 cut(s)	1087
MaeI	CTAG	3 cut(s)	1499, 1562, 1785
MaeII	ACGT	2 cut(s)	592, 1275
MaeIII	GTNAC	7 cut(s)	319, 386, 896, 904, 1102, 1276, 1531
MalI	GATC	4 cut(s)	141, 199, 918, 1875
MbiI	CCGCTC	2 cut(s)	1490, 1617
MboI	GATC	4 cut(s)	139, 197, 916, 1873
MboII	GAAGA	6 cut(s)	413, 458, 656, 711, 745, 1835
MfeI	CAATTG	1 cut(s)	741
MflI	RGATCY	1 cut(s)	197
MhlI	GDGCHC	1 cut(s)	1747
MlsI	TGGCCA	1 cut(s)	1322
MluCI	AATT	7 cut(s)	656, 682, 741, 1042, 1315, 1503, 1650
MluNI	TGGCCA	1 cut(s)	1322
MlyI	GAGTC	3 cut(s)	13, 273, 976
MmeI	TCCRAC	4 cut(s)	205, 256, 907, 1871
Mox20I	TGGCCA	1 cut(s)	1322
Mph1103I	ATGCAT	2 cut(s)	1227, 1697
MroNI	GCCGGC	1 cut(s)	52
MroXI	GAANNNNTTC	1 cut(s)	1379
MscI	TGGCCA	1 cut(s)	1322
MseI	TTAA	9 cut(s)	117, 417, 651, 938, 963, 974, 1175, 1263, 1575
MslI	CAYNNNNRTG	3 cut(s)	1183, 1473, 1694
Msp20I	TGGCCA	1 cut(s)	1322
MspA1I	CMGCKG	1 cut(s)	455
MspI	CCGG	8 cut(s)	53, 535, 641, 721, 1251, 1752, 1838, 1890
MspR9I	CCNGG	4 cut(s)	664, 1089, 1252, 1839
MunI	CAATTG	1 cut(s)	741
MvaI	CCWGG	2 cut(s)	664, 1089
MwoI	GCNNNNNNNGC	9 cut(s)	296, 452, 631, 824, 1031, 1430, 1433, 1439, 1448
NaeI	GCCGGC	1 cut(s)	54
NciI	CCSGG	2 cut(s)	1252, 1839
NcoI	CCATGG	1 cut(s)	1474
NdeI	CATATG	3 cut(s)	1444, 1606, 1691
NdeII	GATC	4 cut(s)	139, 197, 916, 1873
NgoMIV	GCCGGC	1 cut(s)	52
NheI	GCTAGC	1 cut(s)	1784
NlaIII	CATG	9 cut(s)	430, 446, 586, 696, 1013, 1150, 1229, 1478, 1699
NlaIV	GGNNCC	2 cut(s)	994, 1744
NmuCI	GTSAC	2 cut(s)	896, 1102
NsbI	TGCGCA	1 cut(s)	1035
NsiI	ATGCAT	2 cut(s)	1227, 1697
PagI	TCATGA	1 cut(s)	1146
PaqCI	CACCTGC	2 cut(s)	25, 368
PceI	AGGCCT	1 cut(s)	1271
PdiI	GCCGGC	1 cut(s)	54
PdmI	GAANNNNTTC	1 cut(s)	1379
PfeI	GAWTC	3 cut(s)	360, 1755, 1800
PflFI	GACNNNGTC	1 cut(s)	282
PflMI	CCANNNNNTGG	1 cut(s)	356
PinAI	ACCGGT	2 cut(s)	534, 720
PkrI	GCNGC	7 cut(s)	33, 376, 457, 475, 478, 1426, 1435
PleI	GAGTC	3 cut(s)	12, 273, 976
PpsI	GAGTC	3 cut(s)	12, 273, 976
PshBI	ATTAAT	1 cut(s)	974
Psp6I	CCWGG	2 cut(s)	662, 1087
PspEI	GGTNACC	1 cut(s)	896
PspFI	CCCAGC	1 cut(s)	1436
PspGI	CCWGG	2 cut(s)	662, 1087
PspN4I	GGNNCC	2 cut(s)	994, 1744
PspPI	GGNCC	7 cut(s)	347, 752, 826, 1016, 1085, 1212, 1235
PsrI	GAACNNNNNNTAC	2 cut(s)	1813, 1845
PstI	CTGCAG	1 cut(s)	36
PstNI	CAGNNNCTG	1 cut(s)	1112
PsuI	RGATCY	1 cut(s)	197
PsyI	GACNNNGTC	1 cut(s)	282
PvuII	CAGCTG	1 cut(s)	455
RsaI	GTAC	4 cut(s)	1135, 1462, 1661, 1831
RsaNI	GTAC	4 cut(s)	1134, 1461, 1660, 1830
RseI	CAYNNNNRTG	3 cut(s)	1183, 1473, 1694
SaqAI	TTAA	9 cut(s)	117, 417, 651, 938, 963, 974, 1175, 1263, 1575
SatI	GCNGC	7 cut(s)	32, 375, 456, 474, 477, 1425, 1434
Sau3AI	GATC	4 cut(s)	139, 197, 916, 1873
Sau96I	GGNCC	7 cut(s)	347, 752, 826, 1016, 1085, 1212, 1235
SchI	GAGTC	3 cut(s)	13, 273, 976
ScrFI	CCNGG	4 cut(s)	664, 1089, 1252, 1839
SduI	GDGCHC	1 cut(s)	1747
SexAI	ACCWGGT	1 cut(s)	1087
SfaNI	GCATC	2 cut(s)	946, 1438
SfcI	CTRYAG	1 cut(s)	32
SinI	GGWCC	3 cut(s)	347, 1016, 1085
SmiMI	CAYNNNNRTG	3 cut(s)	1183, 1473, 1694
SmlI	CTYRAG	2 cut(s)	727, 986
SmoI	CTYRAG	2 cut(s)	727, 986
Sse9I	AATT	7 cut(s)	656, 682, 741, 1042, 1315, 1503, 1650
SseBI	AGGCCT	1 cut(s)	1271
SsiI	CCGC	7 cut(s)	56, 909, 1233, 1304, 1490, 1508, 1617
SspMI	CTAG	3 cut(s)	1499, 1562, 1785
StuI	AGGCCT	1 cut(s)	1271
StyD4I	CCNGG	4 cut(s)	662, 1087, 1250, 1837
StyI	CCWWGG	3 cut(s)	162, 1474, 1498
TaaI	ACNGT	3 cut(s)	274, 392, 943
TaiI	ACGT	2 cut(s)	595, 1278
TaqI	TCGA	3 cut(s)	63, 81, 1385
TaqII	GACCGA	2 cut(s)	1403, 1892
TasI	AATT	7 cut(s)	656, 682, 741, 1042, 1315, 1503, 1650
TatI	WGTACW	3 cut(s)	1460, 1659, 1829
TfiI	GAWTC	3 cut(s)	360, 1755, 1800
Tru1I	TTAA	9 cut(s)	117, 417, 651, 938, 963, 974, 1175, 1263, 1575
Tru9I	TTAA	9 cut(s)	117, 417, 651, 938, 963, 974, 1175, 1263, 1575
TscAI	CASTG	6 cut(s)	54, 183, 811, 948, 1077, 1888
TseFI	GTSAC	2 cut(s)	896, 1102
TseI	GCWGC	7 cut(s)	31, 374, 455, 473, 476, 1424, 1433
Tsp45I	GTSAC	2 cut(s)	896, 1102
TspDTI	ATGAA	6 cut(s)	431, 965, 998, 1135, 1163, 1792
TspGWI	ACGGA	2 cut(s)	580, 860
TspRI	CASTG	6 cut(s)	54, 183, 811, 948, 1077, 1888
Tth111I	GACNNNGTC	1 cut(s)	282
Van91I	CCANNNNNTGG	1 cut(s)	356
VpaK11BI	GGWCC	3 cut(s)	347, 1016, 1085
VspI	ATTAAT	1 cut(s)	974
XapI	RAATTY	3 cut(s)	682, 1042, 1315
XbaI	TCTAGA	1 cut(s)	1561
XcmI	CCANNNNNNNNNTGG	1 cut(s)	811
XmaJI	CCTAGG	1 cut(s)	1498
XmiI	GTMKAC	2 cut(s)	263, 1494
XmnI	GAANNNNTTC	1 cut(s)	1379
XspI	CTAG	3 cut(s)	1499, 1562, 1785
ZraI	GACGTC	1 cut(s)	593
Zsp2I	ATGCAT	2 cut(s)	1227, 1697

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.