Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr3

Orthogroup ID

OG0005676

Physical Location & Seq

Genomic Coordinates Forward (+)

883307 .. 887181

Gene Structure 3875 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

pycom03g01100.2

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2061 bp

ATGCTCCTCAGTACCCAATCGTTATCGCTCTTCATCTTCAACCAATCAACAACCGAAACCAGAGCGCTTAAAACCCAAACATTTCAGATCAAACCCACCAAGCAAGATTCGAAATTTGACATTTTCGACGACCCCACGATGAGTTCTTCATCCAAAACGGCGAGGAAGCTGCAGGTGGCGTCGCCGGTGCCGGCGGACATCGACATTGCCAACTCTGTCGAGCCTCTCCACATCTCTGAGATTGCAAAAGAGCTTAATCTCAGTCCCGATCACTATCGATCTTTATGGAAAGTACAAGGCCAAGGCCAAGTTTTGTTATCAGTGATTGATGAGCTGAAAGGATCTGGCGATGGGTATTATGTGGTGGTTGGAGGGATTACTCCTACCCCTCTTGGAGAAGGCAAGTCAACCACTACTGTTGGACTCTGTCAAGCTTTGGGTGCTTTTCAGGATAAGAAGGTTGTTACTTGCCTTCGTCAACCATCACAAGGACCAACTTTTGGAATCAAAGGGGGTGCAGCAGGTGGTGGTTATAGTCAGGTGATCCCAATGGATGAGTTTAATCTTCACATGACAGGAGATATTCATGCAATAACAGCTGCAAACAATCTTCTGGCTGCTGCCATTGATACTCGGATTTTCCACGAGGCAACCCAATCGGATAAGGCTCTACTGAACCGGTTGTGTCCACCAAACAAAGAAGGAAAACGGAGTTTCAATGACATCATGTTTAGACGTCTAAAGAAGCTTGGTATCTCCAAGACCACGCCCGAGGAGCTTACACCGGAAGAAGTTAAGCAATTTGCTAGGCTTGATATTGACCCAGATTCCATCACATGGAGAAGAGTAATGGATATAAATGATCGGTTCTTGAGGAAGATTTCAATTGGCCAAGGCCCTGACGAGAAAGGGATGGTAAGAGAAACGGGATTTGATATTTCAGTTGCCAGTGAAATAATGGCAGTTTTGGCCCTTACAACATCTGTAGCGGATATGAGGGAGAGGCTTGGGAAAATGGTGATTGGAAACAGCAGGGCCGGTGACCCTGTAACTGCCGATCACGATCTTGGTGTTGGAGGTGCATTAACAGTGCTAATGAAGGATGCTATTAACCCTACATTAATGCAAACTCTTGAGGGAACCCCAGTTCTTGTTCATGCTGGTCCTTTTGCAAACATTGCACATGGGAATTCCTCTATTGTGGCTGATAAGATTGCACTGAAACTGGTAGGACCAGGTGGGTTTGTAGTCACAGAAGCTGGTTTTGGTGCTGATATTGGTACTGAGAAGTTCATGAACATAAAATGCAGATATAGTGGTTTAACACCACAATGTGCAGTTATAGTTGCAACTATAAGGGCCTTGAAAATGCATGGTGGCGGGCCTGATGTTGTTGCCGGGAAGCCTCTTAACAAGGCCTATGTAACTGAGGATGTGGCTTTGGTTGAGGCGGGTTGTGTGAATTTGGCCAAGCATATTTCAAACACAAAAGCCTATGGAGTGAATGTTGTTGTTGCTGTGAACAAGTTCTCAACTGATAGTGAAGCAGAACTAAATGCAGTCAGAAATGCAGCATTGGCTGCTGGGGCATATGATGCCGTTATTTGCACTCATCATGCCCATGGTGGAAAAGGAGCGGTGGACCTAGGAATTGCAGTTCAGAAAGCCTGCGAGAATGTTACACAACCACTGAAGTTTTTATATCCTCTGGAGTTTAGTATTAAAGAGAAAATTGAAGCAATAGCCAAGTCATATGGCGCGAGTGGTGTTGAATACTCAGAGCAGGCTGAGAAACAAATTGAGATGTACAGCAAGCAAGGGTTTTCTGGTCTGCCCATATGCATGGCGAAAACCCAGTATTCATTTTCACATAATGCTTCTGAGAAAGGAGCCCCCACGGGATTCGTCTTACCAATAAGGGATGTAAGAGCTAGCATTGGAGCTGGATTCATCTATCCTTTGGTGGGAACAATGAGTACAATGCCAGGTCTTCCAACTCGCCCTTGCTTCTATGACATTGATCTTGACACCACAACCGGAAGGGTCATCGGTCTTTCTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004329 GO:0004477 GO:0004488 GO:0005488 GO:0005507 GO:0005575 GO:0005576 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005886 GO:0006082 GO:0006139 GO:0006144 GO:0006575 GO:0006725 GO:0006732 GO:0006760 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009108 GO:0009112 GO:0009113 GO:0009256 GO:0009257 GO:0009396 GO:0009507 GO:0009536 GO:0009719 GO:0009725 GO:0009735 GO:0009987 GO:0010033 GO:0010035 GO:0010038 GO:0016020 GO:0016053 GO:0016491 GO:0016645 GO:0016646 GO:0016787 GO:0016810 GO:0016814 GO:0016874 GO:0016879 GO:0018130 GO:0019238 GO:0019438 GO:0019752 GO:0034641 GO:0042221 GO:0042398 GO:0042440 GO:0042558 GO:0042559 GO:0043167 GO:0043169 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0043603 GO:0043604 GO:0043648 GO:0043650 GO:0044237 GO:0044238 GO:0044249 GO:0044271 GO:0044281 GO:0044283 GO:0044424 GO:0044444 GO:0044464 GO:0046112 GO:0046148 GO:0046394 GO:0046483 GO:0046653 GO:0046654 GO:0046686 GO:0046872 GO:0046914 GO:0048046 GO:0050896 GO:0051186 GO:0051188 GO:0055086 GO:0055114 GO:0071704 GO:0071944 GO:0072521 GO:0072522 GO:1901360 GO:1901362 GO:1901564 GO:1901566 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00670 ko00720 ko01100 ko01120 ko01200 map00670 map00720 map01100 map01120 map01200

Pfam Domains

Protein Families

FTHFS

Protein Analysis

687

Amino Acids

73.33

Weight (kDa)

6.72

Isoelectric Point (pI)

35.68

Instability Index

Protein Domains (Pfam)

No Pfam domains detected for this protein.

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0005676)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G50480
fragaria_vesca	FvH4_3g44470 FvH4_3g44470 FvH4_3g44470 FvH4_3g44470 FvH4_3g44470 FvH4_3g44470 FvH4_3g44470
malus_domestica	MD03G1012400.v1.1 MD11G1015100.v1.1
prunus_persica	Prupe.6G013000_v2.0.a1 Prupe.6G013000_v2.0.a1 Prupe.6G013000_v2.0.a1
pyrus_communis	pycom03g01100 pycom11g01040
rosa_chinensis	RchiOBHm_Chr5g0081161
rosa_laevigata	RLG00000036914
rosa_multiflora	Rmu_sc0016124.1_g000001 Rmu_ssc0000432.1_g000078
rosa_roxburghii	Rroxscaffold_1G00002300
rosa_rugosa	Rorug05G0473600
rosa_samantha	Rh1DG124300 Rh5AG526200 Rh5BG550500 Rh5CG573400 Rh5DG560200
rosa_wichuraiana	Rw1G009600 Rw5G048890

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	2 cut(s)	163, 512
AatII	GACGTC	1 cut(s)	739
Acc36I	ACCTGC	2 cut(s)	163, 512
AccB1I	GGYRCC	1 cut(s)	187
AccB7I	CCANNNNNTGG	2 cut(s)	500, 837
AccBSI	CCGCTC	1 cut(s)	1637
AccII	CGCG	1 cut(s)	1760
AciI	CCGC	5 cut(s)	194, 989, 1380, 1451, 1637
AclWI	GGATC	2 cut(s)	349, 538
AcoI	YGGCCR	2 cut(s)	889, 1467
AcsI	RAATTY	3 cut(s)	113, 1189, 1462
AcuI	CTGAAG	1 cut(s)	1712
AcyI	GRCGYC	2 cut(s)	179, 736
AdeI	CACNNNGTG	1 cut(s)	1334
AfaI	GTAC	5 cut(s)	13, 294, 1282, 1808, 1978
AfeI	AGCGCT	1 cut(s)	66
AfiI	CCNNNNNNNGG	4 cut(s)	488, 500, 837, 1964
AgeI	ACCGGT	1 cut(s)	678
AgsI	TTSAA	7 cut(s)	40, 718, 885, 1366, 1482, 1736, 1772
AjnI	CCWGG	2 cut(s)	1234, 1984
AjuI	GAANNNNNNNTTGG	2 cut(s)	1248, 1280
AloI	GAACNNNNNNTCC	4 cut(s)	1131, 1163, 1641, 1673
AlwI	GGATC	2 cut(s)	349, 538
AlwNI	CAGNNNCTG	1 cut(s)	1259
Ama87I	CYCGRG	1 cut(s)	770
Aor51HI	AGCGCT	1 cut(s)	66
ApeKI	GCWGC	7 cut(s)	169, 518, 599, 617, 620, 1571, 1580
ApoI	RAATTY	3 cut(s)	113, 1189, 1462
AseI	ATTAAT	1 cut(s)	1121
AsiGI	ACCGGT	1 cut(s)	678
Asp700I	GAANNNNTTC	1 cut(s)	1526
AspA2I	CCTAGG	1 cut(s)	1645
AspLEI	GCGC	2 cut(s)	67, 1760
AspS9I	GGNCC	9 cut(s)	491, 896, 970, 1035, 1163, 1232, 1359, 1382, 1642
AsuC2I	CCSGG	1 cut(s)	1399
AsuHPI	GGTGA	3 cut(s)	553, 1030, 1052
AsuII	TTCGAA	1 cut(s)	110
AsuNHI	GCTAGC	1 cut(s)	1931
AvaI	CYCGRG	1 cut(s)	770
AvaII	GGWCC	4 cut(s)	491, 1163, 1232, 1642
AvrII	CCTAGG	1 cut(s)	1645
BalI	TGGCCA	2 cut(s)	891, 1469
BanI	GGYRCC	1 cut(s)	187
BanII	GRGCYC	1 cut(s)	1894
BauI	CACGAG	1 cut(s)	644
BbsI	GAAGAC	1 cut(s)	1982
BbvI	GCAGC	7 cut(s)	156, 530, 586, 604, 607, 1567, 1583
BccI	CCATC	4 cut(s)	344, 490, 839, 907
BceAI	ACGGC	2 cut(s)	174, 1583
BcgI	CGANNNNNNTGC	4 cut(s)	151, 185, 639, 673
BciT130I	CCWGG	2 cut(s)	1236, 1986
BcnI	CCSGG	1 cut(s)	1399
BfaI	CTAG	3 cut(s)	807, 1646, 1932
BfmI	CTRYAG	2 cut(s)	170, 984
BfoI	RGCGCY	1 cut(s)	68
BfuAI	ACCTGC	2 cut(s)	163, 512
BisI	GCNGC	7 cut(s)	170, 519, 600, 618, 621, 1572, 1581
BlnI	CCTAGG	1 cut(s)	1645
BlsI	GCNGC	7 cut(s)	171, 520, 601, 619, 622, 1573, 1582
Bme1390I	CCNGG	3 cut(s)	1236, 1399, 1986
Bme18I	GGWCC	4 cut(s)	491, 1163, 1232, 1642
BmeT110I	CYCGRG	1 cut(s)	770
BmgT120I	GGNCC	9 cut(s)	491, 896, 970, 1035, 1163, 1232, 1359, 1382, 1642
BmiI	GGNNCC	3 cut(s)	189, 1141, 1891
BmrFI	CCNGG	3 cut(s)	1236, 1399, 1986
BmrI	ACTGGG	2 cut(s)	1139, 1849
BmsI	GCATC	2 cut(s)	1093, 1585
BmtI	GCTAGC	1 cut(s)	1935
BmuI	ACTGGG	2 cut(s)	1139, 1849
BpiI	GAAGAC	1 cut(s)	1982
BpmI	CTGGAG	1 cut(s)	1730
Bpu14I	TTCGAA	1 cut(s)	110
BpuEI	CTTGAG	2 cut(s)	892, 1154
BpuMI	CCSGG	1 cut(s)	1399
Bsa29I	ATCGAT	1 cut(s)	277
BsaBI	GATNNNNATC	2 cut(s)	273, 1062
BsaHI	GRCGYC	2 cut(s)	179, 736
BsaJI	CCNNGG	6 cut(s)	301, 771, 892, 1621, 1645, 1896
BsaWI	WCCGGW	3 cut(s)	678, 784, 2036
BsaXI	ACNNNNNCTCC	2 cut(s)	1491, 1521
Bsc4I	CCNNNNNNNGG	4 cut(s)	488, 500, 837, 1964
Bse118I	RCCGGY	4 cut(s)	184, 190, 678, 1037
Bse1I	ACTGG	4 cut(s)	948, 1145, 1230, 1855
Bse3DI	GCAATG	2 cut(s)	204, 1176
Bse8I	GATNNNNATC	2 cut(s)	273, 1062
BseBI	CCWGG	2 cut(s)	1236, 1986
BseCI	ATCGAT	1 cut(s)	277
BseDI	CCNNGG	6 cut(s)	301, 771, 892, 1621, 1645, 1896
BseGI	GGATG	6 cut(s)	149, 559, 918, 1108, 1438, 1927
BseJI	GATNNNNATC	2 cut(s)	273, 1062
BseLI	CCNNNNNNNGG	4 cut(s)	488, 500, 837, 1964
BseMI	GCAATG	2 cut(s)	204, 1176
BseMII	CTCAG	8 cut(s)	22, 228, 274, 1275, 1419, 1779, 1791, 1872
BseNI	ACTGG	4 cut(s)	948, 1145, 1230, 1855
BseRI	GAGGAG	1 cut(s)	788
BseXI	GCAGC	7 cut(s)	156, 530, 586, 604, 607, 1567, 1583
BseYI	CCCAGC	1 cut(s)	1583
BsgI	GTGCAG	2 cut(s)	537, 1356
Bsh1236I	CGCG	1 cut(s)	1760
BshNI	GGYRCC	1 cut(s)	187
BshTI	ACCGGT	1 cut(s)	678
BshVI	ATCGAT	1 cut(s)	277
BsiHKCI	CYCGRG	1 cut(s)	770
BsiSI	CCGG	7 cut(s)	185, 191, 679, 785, 1038, 1398, 2037
BslFI	GGGAC	1 cut(s)	249
BslI	CCNNNNNNNGG	4 cut(s)	488, 500, 837, 1964
BsmFI	GGGAC	1 cut(s)	249
BsoBI	CYCGRG	1 cut(s)	770
Bsp119I	TTCGAA	1 cut(s)	110
Bsp1286I	GDGCHC	1 cut(s)	1894
Bsp1407I	TGTACA	1 cut(s)	1806
Bsp143I	GATC	9 cut(s)	87, 268, 278, 341, 543, 862, 1057, 1063, 2020
Bsp19I	CCATGG	1 cut(s)	1621
BspACI	CCGC	5 cut(s)	194, 989, 1380, 1451, 1637
BspCNI	CTCAG	8 cut(s)	21, 229, 273, 1276, 1420, 1780, 1790, 1873
BspDI	ATCGAT	1 cut(s)	277
BspFNI	CGCG	1 cut(s)	1760
BspHI	TCATGA	1 cut(s)	1293
BspLI	GGNNCC	3 cut(s)	189, 1141, 1891
BspMAI	CTGCAG	1 cut(s)	174
BspMI	ACCTGC	2 cut(s)	163, 512
BspOI	GCTAGC	1 cut(s)	1935
BspPI	GGATC	2 cut(s)	349, 538
BspQI	GCTCTTC	1 cut(s)	35
BspT104I	TTCGAA	1 cut(s)	110
BspT107I	GGYRCC	1 cut(s)	187
BsrBI	CCGCTC	1 cut(s)	1637
BsrDI	GCAATG	2 cut(s)	204, 1176
BsrFI	RCCGGY	4 cut(s)	184, 190, 678, 1037
BsrGI	TGTACA	1 cut(s)	1806
BsrI	ACTGG	4 cut(s)	948, 1145, 1230, 1855
BssAI	RCCGGY	4 cut(s)	184, 190, 678, 1037
BssECI	CCNNGG	6 cut(s)	301, 771, 892, 1621, 1645, 1896
BssMI	GATC	9 cut(s)	87, 268, 278, 341, 543, 862, 1057, 1063, 2020
BssNI	GRCGYC	2 cut(s)	179, 736
BssSI	CACGAG	1 cut(s)	644
BssT1I	CCWWGG	4 cut(s)	301, 892, 1621, 1645
Bst2BI	CACGAG	1 cut(s)	644
Bst2UI	CCWGG	2 cut(s)	1236, 1986
Bst4CI	ACNGT	2 cut(s)	418, 1090
Bst6I	CTCTTC	2 cut(s)	35, 838
BstACI	GRCGYC	2 cut(s)	179, 736
BstAPI	GCANNNNNTGC	3 cut(s)	1178, 1580, 1595
BstAUI	TGTACA	1 cut(s)	1806
BstBI	TTCGAA	1 cut(s)	110
BstC8I	GCNNGC	6 cut(s)	192, 1382, 1669, 1785, 1814, 1933
BstDEI	CTNAG	8 cut(s)	8, 237, 260, 1284, 1428, 1777, 1788, 1881
BstDSI	CCRYGG	2 cut(s)	1621, 1896
BstEII	GGTNACC	1 cut(s)	1040
BstF5I	GGATG	6 cut(s)	149, 559, 918, 1108, 1438, 1927
BstFNI	CGCG	1 cut(s)	1760
BstH2I	RGCGCY	1 cut(s)	68
BstHHI	GCGC	2 cut(s)	67, 1760
BstKTI	GATC	9 cut(s)	90, 271, 281, 344, 546, 865, 1060, 1066, 2023
BstMBI	GATC	9 cut(s)	87, 268, 278, 341, 543, 862, 1057, 1063, 2020
BstMWI	GCNNNNNNNGC	9 cut(s)	440, 596, 775, 968, 1178, 1577, 1580, 1586, 1595
BstNI	CCWGG	2 cut(s)	1236, 1986
BstPI	GGTNACC	1 cut(s)	1040
BstSCI	CCNGG	3 cut(s)	1234, 1397, 1984
BstSFI	CTRYAG	2 cut(s)	170, 984
BstUI	CGCG	1 cut(s)	1760
BstV1I	GCAGC	7 cut(s)	156, 530, 586, 604, 607, 1567, 1583
BstV2I	GAAGAC	1 cut(s)	1982
BstX2I	RGATCY	1 cut(s)	341
BstXI	CCANNNNNNTGG	1 cut(s)	1843
BstYI	RGATCY	1 cut(s)	341
Bsu15I	ATCGAT	1 cut(s)	277
BsuTUI	ATCGAT	1 cut(s)	277
BtgI	CCRYGG	2 cut(s)	1621, 1896
BtgZI	GCGATG	1 cut(s)	363
BtsCI	GGATG	6 cut(s)	149, 559, 918, 1108, 1438, 1927
BtsIMutI	CAGTG	5 cut(s)	327, 955, 1095, 1217, 1688
BveI	ACCTGC	2 cut(s)	163, 512
Cac8I	GCNNGC	6 cut(s)	192, 1382, 1669, 1785, 1814, 1933
CaiI	CAGNNNCTG	1 cut(s)	1259
CciI	TCATGA	1 cut(s)	1293
CfoI	GCGC	2 cut(s)	67, 1760
Cfr10I	RCCGGY	4 cut(s)	184, 190, 678, 1037
Cfr13I	GGNCC	9 cut(s)	491, 896, 970, 1035, 1163, 1232, 1359, 1382, 1642
ClaI	ATCGAT	1 cut(s)	277
CseI	GACGC	1 cut(s)	168
CsiI	ACCWGGT	1 cut(s)	1234
Csp6I	GTAC	5 cut(s)	12, 293, 1281, 1807, 1977
CspAI	ACCGGT	1 cut(s)	678
CspCI	CAANNNNNGTGG	2 cut(s)	400, 435
CviQI	GTAC	5 cut(s)	12, 293, 1281, 1807, 1977
DdeI	CTNAG	8 cut(s)	8, 237, 260, 1284, 1428, 1777, 1788, 1881
DpnI	GATC	9 cut(s)	89, 270, 280, 343, 545, 864, 1059, 1065, 2022
DpnII	GATC	9 cut(s)	87, 268, 278, 341, 543, 862, 1057, 1063, 2020
DraIII	CACNNNGTG	1 cut(s)	1334
EaeI	YGGCCR	2 cut(s)	889, 1467
Eam1104I	CTCTTC	2 cut(s)	35, 838
EarI	CTCTTC	2 cut(s)	35, 838
EciI	GGCGGA	1 cut(s)	209
Eco130I	CCWWGG	4 cut(s)	301, 892, 1621, 1645
Eco147I	AGGCCT	1 cut(s)	1418
Eco24I	GRGCYC	1 cut(s)	1894
Eco47I	GGWCC	4 cut(s)	491, 1163, 1232, 1642
Eco47III	AGCGCT	1 cut(s)	66
Eco57I	CTGAAG	1 cut(s)	1712
Eco88I	CYCGRG	1 cut(s)	770
Eco91I	GGTNACC	1 cut(s)	1040
EcoO109I	RGGNCCY	2 cut(s)	896, 1359
EcoO65I	GGTNACC	1 cut(s)	1040
EcoRI	GAATTC	1 cut(s)	1189
EcoRII	CCWGG	2 cut(s)	1234, 1984
EcoT14I	CCWWGG	4 cut(s)	301, 892, 1621, 1645
EcoT22I	ATGCAT	2 cut(s)	1374, 1844
EcoT38I	GRGCYC	1 cut(s)	1894
ErhI	CCWWGG	4 cut(s)	301, 892, 1621, 1645
FaqI	GGGAC	1 cut(s)	249
FauI	CCCGC	2 cut(s)	1373, 1444
FauNDI	CATATG	3 cut(s)	1591, 1753, 1838
Fnu4HI	GCNGC	7 cut(s)	170, 519, 600, 618, 621, 1572, 1581
FokI	GGATG	6 cut(s)	136, 566, 925, 1115, 1445, 1934
FriOI	GRGCYC	1 cut(s)	1894
Fsp4HI	GCNGC	7 cut(s)	170, 519, 600, 618, 621, 1572, 1581
FspBI	CTAG	3 cut(s)	807, 1646, 1932
GlaI	GCGC	2 cut(s)	66, 1759
GluI	GCNGC	7 cut(s)	170, 519, 600, 618, 621, 1572, 1581
GsaI	CCCAGC	1 cut(s)	1587
GsuI	CTGGAG	1 cut(s)	1730
HaeII	RGCGCY	1 cut(s)	68
HapII	CCGG	7 cut(s)	185, 191, 679, 785, 1038, 1398, 2037
HgaI	GACGC	1 cut(s)	168
HhaI	GCGC	2 cut(s)	67, 1760
Hin1I	GRCGYC	2 cut(s)	179, 736
Hin6I	GCGC	2 cut(s)	65, 1758
HinP1I	GCGC	2 cut(s)	65, 1758
HincII	GTYRAC	2 cut(s)	408, 479
HindII	GTYRAC	2 cut(s)	408, 479
HindIII	AAGCTT	2 cut(s)	432, 746
HinfI	GANTC	6 cut(s)	107, 423, 504, 827, 1902, 1947
HpaII	CCGG	7 cut(s)	185, 191, 679, 785, 1038, 1398, 2037
HphI	GGTGA	3 cut(s)	553, 1030, 1052
Hpy166II	GTNNAC	5 cut(s)	408, 479, 689, 1522, 1642
Hpy188I	TCNGA	8 cut(s)	87, 238, 636, 661, 1565, 1662, 1780, 1882
Hpy188III	TCNNGA	9 cut(s)	266, 449, 871, 1061, 1133, 1294, 1709, 2024, 2058
Hpy8I	GTNNAC	5 cut(s)	408, 479, 689, 1522, 1642
Hpy99I	CGWCG	2 cut(s)	131, 184
HpyAV	CCTTC	6 cut(s)	392, 451, 482, 695, 1093, 2034
HpyCH4III	ACNGT	2 cut(s)	418, 1090
HpyCH4IV	ACGT	1 cut(s)	736
HpyF10VI	GCNNNNNNNGC	9 cut(s)	440, 596, 775, 968, 1178, 1577, 1580, 1586, 1595
HpyF3I	CTNAG	8 cut(s)	8, 237, 260, 1284, 1428, 1777, 1788, 1881
HpySE526I	ACGT	1 cut(s)	736
Hsp92I	GRCGYC	2 cut(s)	179, 736
HspAI	GCGC	2 cut(s)	65, 1758
KroI	GCCGGC	1 cut(s)	190
KroNI	GCCGGC	1 cut(s)	192
Kzo9I	GATC	9 cut(s)	87, 268, 278, 341, 543, 862, 1057, 1063, 2020
LguI	GCTCTTC	1 cut(s)	35
LmnI	GCTCC	5 cut(s)	9, 775, 1634, 1889, 1940
Lsp1109I	GCAGC	7 cut(s)	156, 530, 586, 604, 607, 1567, 1583
LweI	GCATC	2 cut(s)	1093, 1585
MabI	ACCWGGT	1 cut(s)	1234
MaeI	CTAG	3 cut(s)	807, 1646, 1932
MaeII	ACGT	1 cut(s)	736
MaeIII	GTNAC	6 cut(s)	463, 1040, 1048, 1249, 1423, 1678
MalI	GATC	9 cut(s)	89, 270, 280, 343, 545, 864, 1059, 1065, 2022
MbiI	CCGCTC	1 cut(s)	1637
MboI	GATC	9 cut(s)	87, 268, 278, 341, 543, 862, 1057, 1063, 2020
MboII	GAAGA	9 cut(s)	22, 28, 138, 557, 602, 800, 855, 889, 1982
MfeI	CAATTG	1 cut(s)	885
MflI	RGATCY	1 cut(s)	341
MhlI	GDGCHC	1 cut(s)	1894
MlsI	TGGCCA	2 cut(s)	891, 1469
MluCI	AATT	8 cut(s)	113, 800, 885, 1189, 1462, 1650, 1731, 1797
MluNI	TGGCCA	2 cut(s)	891, 1469
MlyI	GAGTC	1 cut(s)	417
MmeI	TCCRAC	4 cut(s)	349, 400, 1054, 2018
Mox20I	TGGCCA	2 cut(s)	891, 1469
Mph1103I	ATGCAT	2 cut(s)	1374, 1844
MroNI	GCCGGC	1 cut(s)	190
MroXI	GAANNNNTTC	1 cut(s)	1526
MscI	TGGCCA	2 cut(s)	891, 1469
MslI	CAYNNNNRTG	3 cut(s)	1330, 1620, 1841
Msp20I	TGGCCA	2 cut(s)	891, 1469
MspA1I	CMGCKG	1 cut(s)	599
MspI	CCGG	7 cut(s)	185, 191, 679, 785, 1038, 1398, 2037
MspR9I	CCNGG	3 cut(s)	1236, 1399, 1986
MunI	CAATTG	1 cut(s)	885
MvaI	CCWGG	2 cut(s)	1236, 1986
MvnI	CGCG	1 cut(s)	1760
MwoI	GCNNNNNNNGC	9 cut(s)	440, 596, 775, 968, 1178, 1577, 1580, 1586, 1595
NaeI	GCCGGC	1 cut(s)	192
NciI	CCSGG	1 cut(s)	1399
NcoI	CCATGG	1 cut(s)	1621
NdeI	CATATG	3 cut(s)	1591, 1753, 1838
NdeII	GATC	9 cut(s)	87, 268, 278, 341, 543, 862, 1057, 1063, 2020
NgoMIV	GCCGGC	1 cut(s)	190
NheI	GCTAGC	1 cut(s)	1931
NlaIV	GGNNCC	3 cut(s)	189, 1141, 1891
NmuCI	GTSAC	2 cut(s)	1040, 1249
NsiI	ATGCAT	2 cut(s)	1374, 1844
NspV	TTCGAA	1 cut(s)	110
PagI	TCATGA	1 cut(s)	1293
PaqCI	CACCTGC	2 cut(s)	163, 512
PceI	AGGCCT	1 cut(s)	1418
PciSI	GCTCTTC	1 cut(s)	35
PdiI	GCCGGC	1 cut(s)	192
PdmI	GAANNNNTTC	1 cut(s)	1526
PfeI	GAWTC	5 cut(s)	107, 504, 827, 1902, 1947
PflFI	GACNNNGTC	1 cut(s)	426
PflMI	CCANNNNNTGG	2 cut(s)	500, 837
PinAI	ACCGGT	1 cut(s)	678
PkrI	GCNGC	7 cut(s)	171, 520, 601, 619, 622, 1573, 1582
PleI	GAGTC	1 cut(s)	417
PpsI	GAGTC	1 cut(s)	417
PshBI	ATTAAT	1 cut(s)	1121
Psp6I	CCWGG	2 cut(s)	1234, 1984
PspEI	GGTNACC	1 cut(s)	1040
PspFI	CCCAGC	1 cut(s)	1583
PspGI	CCWGG	2 cut(s)	1234, 1984
PspN4I	GGNNCC	3 cut(s)	189, 1141, 1891
PspPI	GGNCC	9 cut(s)	491, 896, 970, 1035, 1163, 1232, 1359, 1382, 1642
PsrI	GAACNNNNNNTAC	2 cut(s)	1960, 1992
PstI	CTGCAG	1 cut(s)	174
PstNI	CAGNNNCTG	1 cut(s)	1259
PsuI	RGATCY	1 cut(s)	341
PsyI	GACNNNGTC	1 cut(s)	426
PvuII	CAGCTG	1 cut(s)	599
RsaI	GTAC	5 cut(s)	13, 294, 1282, 1808, 1978
RsaNI	GTAC	5 cut(s)	12, 293, 1281, 1807, 1977
RseI	CAYNNNNRTG	3 cut(s)	1330, 1620, 1841
SapI	GCTCTTC	1 cut(s)	35
SatI	GCNGC	7 cut(s)	170, 519, 600, 618, 621, 1572, 1581
Sau3AI	GATC	9 cut(s)	87, 268, 278, 341, 543, 862, 1057, 1063, 2020
Sau96I	GGNCC	9 cut(s)	491, 896, 970, 1035, 1163, 1232, 1359, 1382, 1642
SchI	GAGTC	1 cut(s)	417
ScrFI	CCNGG	3 cut(s)	1236, 1399, 1986
SduI	GDGCHC	1 cut(s)	1894
SexAI	ACCWGGT	1 cut(s)	1234
SfaNI	GCATC	2 cut(s)	1093, 1585
SfcI	CTRYAG	2 cut(s)	170, 984
SfuI	TTCGAA	1 cut(s)	110
SgrAI	CRCCGGYG	1 cut(s)	184
SinI	GGWCC	4 cut(s)	491, 1163, 1232, 1642
SmiMI	CAYNNNNRTG	3 cut(s)	1330, 1620, 1841
SmlI	CTYRAG	2 cut(s)	871, 1133
SmoI	CTYRAG	2 cut(s)	871, 1133
Sse9I	AATT	8 cut(s)	113, 800, 885, 1189, 1462, 1650, 1731, 1797
SseBI	AGGCCT	1 cut(s)	1418
SsiI	CCGC	5 cut(s)	194, 989, 1380, 1451, 1637
SspMI	CTAG	3 cut(s)	807, 1646, 1932
StuI	AGGCCT	1 cut(s)	1418
StyD4I	CCNGG	3 cut(s)	1234, 1397, 1984
StyI	CCWWGG	4 cut(s)	301, 892, 1621, 1645
TaaI	ACNGT	2 cut(s)	418, 1090
TaiI	ACGT	1 cut(s)	739
TaqI	TCGA	5 cut(s)	110, 126, 201, 219, 277
TaqII	GACCGA	1 cut(s)	2039
TasI	AATT	8 cut(s)	113, 800, 885, 1189, 1462, 1650, 1731, 1797
TatI	WGTACW	3 cut(s)	292, 1806, 1976
TfiI	GAWTC	5 cut(s)	107, 504, 827, 1902, 1947
TscAI	CASTG	5 cut(s)	327, 955, 1095, 1224, 1695
TseFI	GTSAC	2 cut(s)	1040, 1249
TseI	GCWGC	7 cut(s)	169, 518, 599, 617, 620, 1571, 1580
Tsp45I	GTSAC	2 cut(s)	1040, 1249
TspDTI	ATGAA	9 cut(s)	22, 138, 575, 1112, 1145, 1282, 1310, 1851, 1939
TspGWI	ACGGA	1 cut(s)	724
TspRI	CASTG	5 cut(s)	327, 955, 1095, 1224, 1695
Tth111I	GACNNNGTC	1 cut(s)	426
Van91I	CCANNNNNTGG	2 cut(s)	500, 837
VpaK11BI	GGWCC	4 cut(s)	491, 1163, 1232, 1642
VspI	ATTAAT	1 cut(s)	1121
XapI	RAATTY	3 cut(s)	113, 1189, 1462
XcmI	CCANNNNNNNNNTGG	1 cut(s)	955
XmaJI	CCTAGG	1 cut(s)	1645
XmnI	GAANNNNTTC	1 cut(s)	1526
XspI	CTAG	3 cut(s)	807, 1646, 1932
ZraI	GACGTC	1 cut(s)	737
Zsp2I	ATGCAT	2 cut(s)	1374, 1844

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.