Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr5A

Orthogroup ID

OG0005676

Physical Location & Seq

Genomic Coordinates Reverse (-)

90257618 .. 90262363

Gene Structure 4746 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh5AG526200.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2121 bp

ATGCGACTCTATCAGTGTTCACCAACTGTCCCAGTTAGAGCTTTAGCCTCCAATCCTGGAATCTCAGTCGATAAGAAATCAGGGCACTCCATTCTATGTTCTTCAAGCCTCACTTTCCCATCATCAATAGGTCAACCTCAACAAACAGAACCACTTAAAACAGATCCATCAATCCCAGAAACCAGTTTTCACAGATTCTGGGAGCCCAAAATGAGCCCATCAAAGACCACTAGGAAGTTGGAGGTGGCTTCACCAGTCCCAGCTGACATAGACATTGCCAATTCAGTTGAGCCTGTTCACATCTCTGAGATTGCCAAAGACCTTAATCTCAGTCCTGACCACTATGATCTCTATGGAAAATACAAGGCCAAGGTTTTGTTATCTGTGATTGATGAACTGAAAGGATCTAGTGATGGGTATTATGTTGTTGTGGGAGGGATTACTCCTACACCTCTTGGAGAAGGCAAGTCTACCACCACTGTTGGACTGTGTCAAGCTTTGGGTGCTTTTCAAGATAAGAAGGTTGTCACTTGCCTTCGTCAACCATCACAAGGACCAACTTTTGGAATTAAAGGTGGTGCAGCAGGTGGTGGTTACAGTCAAGTGATCCCAATGGATGAGTTTAATCTGCACTTAACAGGAGATATTCACGCAATAACAGCTGCAAACAATCTTCTAGCTGCTGCCATTGATACTCGAATGTTCCATGAAGCAACCCAATCAGATAAGGCTCTAATGAACCGATTATGCCCACCAAACAAAGAAGGAAAAAAGAGCTTTAATGATATCATGTTTAGACGTCTAAAGAAGCTGGGTATCTCCAAGACACAGCCTGAGGACCTTACACCAGAAGAAGTTAAGAAATTTGCTAGGCTTGATATTGATCCAGATTCTATCACCTGGAGAAGAGTAATGGATATAAATGACCGGCTGTTGAGAAAAATAACAGTTGGCCAGGGCCCTGAAGAGAAAGGGATGGTGAGAGAAACAGGATTTGATATTTCAGTTGCCAGTGAGATAATGGCAGTTTTGGCCCTTACGACATCTCTAGCAGATATGAGGGAGAGGCTTGGCAAAATGGTAATTGGAAACAGCAGGGCTGGTGACCCAGTTACTGCCGATGATCTTGGTGTCGGAGGTGCATTAACGGTTCTAATGAAGGATGCTATTAACCCTACATTAATGCAGACTCTTGAGGGGACCCCAGTTCTTGTTCATGCTGGTCCTTTTGCAAATATTGCCCATGGAAATTCCTCCATTGTGGCTGATAAGATTGCGCTAAAGCTGGTGGGACCAGGTGGGTTTGTAGTCACAGAAGCTGGTTTTGGTGCTGATATTGGCACTGAGAAATTTATGAATATAAAATGCCGGTATAGTGGTTTAACACCGCAGTGCGCAATTATAGTTGCCACCATAAGGGCCTTGAAAATGCATGGTGGTGGCCCGGATGTTGTTGCTGGGAAGCCTCTTAATAGTGCCTACATAACTGAGAATGTGGCTCTGGTTGAGGCTGGCTGTGTGAATTTGGCCAGGCATATTACAAATACAAAAGCCTATGGTGTGAATGTTGTTGTTGCCGTGAACAAATTCTCAACTGACTCTGACGCAGAATTGAATGCAGTTCGAAATGCAGCACTGGCTGCTGGGGCATATGATGCTGTGATATGTACTCACCATGCCCATGGTGGAAAAGGAGCGGTAGACCTAGGGATTGCGGTTCAGAAAGCTTGTGAGAATGTTACACAACCATTAAAGTTTTTATATCCTTTGGACTTCAGTATTAAAGAAAAGATAGAGGCAATAGCCAGGTCATATGGTGCAAGTGGTGTTGAATACTCGGAGCAGGCTGAAAAGCAGATTGCAATGTACAGCAGGCAAGGGTTTTCTGGTCTGCCAATATGCATGGCAAAGACCCAGTATTCATTTTCTGATAAAGCTACTGCGAAAGGAGCACCATCCAATTTTGTCTTACCGATAAGGGATGTGAGGGCAAGCATTGGAGCTGGATTTATATATCCTTTGGTAGGAACCATGAGCACAATGCCAGGACTTCCAACTAGGCCTTGCTTTTATGATATTGATCTTGACACCACCACTGGAAGGGTTATTGGTCTTTCTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004329 GO:0004477 GO:0004488 GO:0005488 GO:0005507 GO:0005575 GO:0005576 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005886 GO:0006082 GO:0006139 GO:0006144 GO:0006575 GO:0006725 GO:0006732 GO:0006760 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009108 GO:0009112 GO:0009113 GO:0009256 GO:0009257 GO:0009396 GO:0009507 GO:0009536 GO:0009719 GO:0009725 GO:0009735 GO:0009987 GO:0010033 GO:0010035 GO:0010038 GO:0016020 GO:0016053 GO:0016491 GO:0016645 GO:0016646 GO:0016787 GO:0016810 GO:0016814 GO:0016874 GO:0016879 GO:0018130 GO:0019238 GO:0019438 GO:0019752 GO:0034641 GO:0042221 GO:0042398 GO:0042440 GO:0042558 GO:0042559 GO:0043167 GO:0043169 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0043603 GO:0043604 GO:0043648 GO:0043650 GO:0044237 GO:0044238 GO:0044249 GO:0044271 GO:0044281 GO:0044283 GO:0044424 GO:0044444 GO:0044464 GO:0046112 GO:0046148 GO:0046394 GO:0046483 GO:0046653 GO:0046654 GO:0046686 GO:0046872 GO:0046914 GO:0048046 GO:0050896 GO:0051186 GO:0051188 GO:0055086 GO:0055114 GO:0071704 GO:0071944 GO:0072521 GO:0072522 GO:1901360 GO:1901362 GO:1901564 GO:1901566 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00670 ko00720 ko01100 ko01120 ko01200 map00670 map00720 map01100 map01120 map01200

Pfam Domains

Protein Families

FTHFS

Protein Analysis

706

Amino Acids

75.32

Weight (kDa)

7.88

Isoelectric Point (pI)

35.43

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
FTHFS	PF01268	88 - 706	4e-252	Formate--tetrahydrofolate ligase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0005676)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G50480
fragaria_vesca	FvH4_3g44470 FvH4_3g44470 FvH4_3g44470 FvH4_3g44470 FvH4_3g44470 FvH4_3g44470 FvH4_3g44470
malus_domestica	MD03G1012400.v1.1 MD11G1015100.v1.1
prunus_persica	Prupe.6G013000_v2.0.a1 Prupe.6G013000_v2.0.a1 Prupe.6G013000_v2.0.a1
pyrus_communis	pycom03g01100 pycom11g01040
rosa_chinensis	RchiOBHm_Chr5g0081161
rosa_laevigata	RLG00000036914
rosa_multiflora	Rmu_sc0016124.1_g000001 Rmu_ssc0000432.1_g000078
rosa_roxburghii	Rroxscaffold_1G00002300
rosa_rugosa	Rorug05G0473600
rosa_samantha	Rh1DG124300 Rh5AG526200 Rh5BG550500 Rh5CG573400 Rh5DG560200
rosa_wichuraiana	Rw1G009600 Rw5G048890

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	575
AatII	GACGTC	1 cut(s)	802
Acc16I	TGCGCA	1 cut(s)	1396
Acc36I	ACCTGC	1 cut(s)	575
AccB7I	CCANNNNNTGG	1 cut(s)	563
AccBSI	CCGCTC	1 cut(s)	1697
AccI	GTMKAC	2 cut(s)	470, 1701
AciI	CCGC	3 cut(s)	1388, 1697, 1715
AclWI	GGATC	4 cut(s)	158, 412, 601, 878
AcoI	YGGCCR	2 cut(s)	952, 1527
AcsI	RAATTY	5 cut(s)	863, 1249, 1349, 1522, 1586
AcuI	CTGAAG	2 cut(s)	984, 1759
AcyI	GRCGYC	1 cut(s)	799
AfaI	GTAC	2 cut(s)	1669, 1868
AfiI	CCNNNNNNNGG	5 cut(s)	551, 563, 1416, 2024, 2100
AgsI	TTSAA	5 cut(s)	105, 512, 1426, 1615, 1832
AjnI	CCWGG	7 cut(s)	55, 899, 954, 1294, 1529, 1805, 2044
AjuI	GAANNNNNNNTTGG	2 cut(s)	1308, 1340
AleI	CACNNNNGTG	1 cut(s)	1390
Alw21I	GWGCWC	2 cut(s)	1954, 2039
AlwI	GGATC	4 cut(s)	158, 412, 601, 878
AlwNI	CAGNNNCTG	3 cut(s)	198, 1115, 1319
AoxI	GGCC	8 cut(s)	366, 952, 958, 1032, 1419, 1441, 1527, 2060
ApaI	GGGCCC	1 cut(s)	962
ApeKI	GCWGC	6 cut(s)	581, 662, 680, 683, 1631, 1640
ApoI	RAATTY	5 cut(s)	863, 1249, 1349, 1522, 1586
AseI	ATTAAT	1 cut(s)	1181
Asp700I	GAANNNNTTC	1 cut(s)	1586
AspA2I	CCTAGG	1 cut(s)	1705
AspLEI	GCGC	2 cut(s)	1279, 1397
AsuC2I	CCSGG	1 cut(s)	1445
AsuHPI	GGTGA	6 cut(s)	12, 243, 889, 991, 1115, 1664
AsuII	TTCGAA	1 cut(s)	1624
AvaII	GGWCC	5 cut(s)	554, 838, 1200, 1223, 1292
AvrII	CCTAGG	1 cut(s)	1705
AxyI	CCTNAGG	1 cut(s)	834
BaeGI	GKGCMC	2 cut(s)	87, 962
BalI	TGGCCA	2 cut(s)	954, 1529
BanII	GRGCYC	3 cut(s)	207, 218, 962
Bbv12I	GWGCWC	2 cut(s)	1954, 2039
BbvI	GCAGC	6 cut(s)	593, 649, 667, 670, 1627, 1643
BccI	CCATC	7 cut(s)	127, 175, 226, 407, 553, 970, 1963
BceAI	ACGGC	1 cut(s)	1562
BciT130I	CCWGG	7 cut(s)	57, 901, 956, 1296, 1531, 1807, 2046
BcnI	CCSGG	1 cut(s)	1445
BfaI	CTAG	7 cut(s)	231, 408, 677, 870, 1049, 1706, 2058
BfuAI	ACCTGC	1 cut(s)	575
BisI	GCNGC	6 cut(s)	582, 663, 681, 684, 1632, 1641
BlnI	CCTAGG	1 cut(s)	1705
BlsI	GCNGC	6 cut(s)	583, 664, 682, 685, 1633, 1642
Bme1390I	CCNGG	8 cut(s)	57, 901, 956, 1296, 1445, 1531, 1807, 2046
Bme18I	GGWCC	5 cut(s)	554, 838, 1200, 1223, 1292
BmiI	GGNNCC	6 cut(s)	204, 960, 1201, 1202, 1293, 2029
BmrFI	CCNGG	8 cut(s)	57, 901, 956, 1296, 1445, 1531, 1807, 2046
BmrI	ACTGGG	4 cut(s)	26, 1103, 1199, 1909
BmsI	GCATC	2 cut(s)	1153, 1645
BmuI	ACTGGG	4 cut(s)	26, 1103, 1199, 1909
BpmI	CTGGAG	1 cut(s)	922
Bpu14I	TTCGAA	1 cut(s)	1624
BpuEI	CTTGAG	1 cut(s)	1214
BpuMI	CCSGG	1 cut(s)	1445
BsaBI	GATNNNNATC	2 cut(s)	882, 2079
BsaHI	GRCGYC	1 cut(s)	799
BsaJI	CCNNGG	5 cut(s)	369, 955, 1243, 1681, 1705
Bsc4I	CCNNNNNNNGG	5 cut(s)	551, 563, 1416, 2024, 2100
Bse118I	RCCGGY	2 cut(s)	927, 1368
Bse1I	ACTGG	9 cut(s)	32, 183, 254, 1011, 1109, 1205, 1641, 1915, 2101
Bse21I	CCTNAGG	1 cut(s)	834
Bse3DI	GCAATG	2 cut(s)	273, 1869
Bse8I	GATNNNNATC	2 cut(s)	882, 2079
BseBI	CCWGG	7 cut(s)	57, 901, 956, 1296, 1531, 1807, 2046
BseDI	CCNNGG	5 cut(s)	369, 955, 1243, 1681, 1705
BseGI	GGATG	6 cut(s)	622, 981, 1168, 1453, 1955, 1987
BseJI	GATNNNNATC	2 cut(s)	882, 2079
BseLI	CCNNNNNNNGG	5 cut(s)	551, 563, 1416, 2024, 2100
BseMI	GCAATG	2 cut(s)	273, 1869
BseMII	CTCAG	6 cut(s)	78, 297, 343, 825, 1335, 1479
BseNI	ACTGG	9 cut(s)	32, 183, 254, 1011, 1109, 1205, 1641, 1915, 2101
BseSI	GKGCMC	2 cut(s)	87, 962
BseXI	GCAGC	6 cut(s)	593, 649, 667, 670, 1627, 1643
BseYI	CCCAGC	4 cut(s)	259, 811, 1457, 1643
BsgI	GTGCAG	2 cut(s)	600, 614
BshFI	GGCC	8 cut(s)	368, 954, 960, 1034, 1421, 1443, 1529, 2062
BsiHKAI	GWGCWC	2 cut(s)	1954, 2039
BsiSI	CCGG	3 cut(s)	928, 1369, 1445
BslFI	GGGAC	4 cut(s)	14, 242, 1213, 1305
BslI	CCNNNNNNNGG	5 cut(s)	551, 563, 1416, 2024, 2100
BsmFI	GGGAC	4 cut(s)	14, 242, 1213, 1305
BsmI	GAATGC	1 cut(s)	1621
BsnI	GGCC	8 cut(s)	368, 954, 960, 1034, 1421, 1443, 1529, 2062
Bsp119I	TTCGAA	1 cut(s)	1624
Bsp120I	GGGCCC	1 cut(s)	958
Bsp1286I	GDGCHC	6 cut(s)	87, 207, 218, 962, 1954, 2039
Bsp1407I	TGTACA	1 cut(s)	1866
Bsp143I	GATC	7 cut(s)	163, 346, 404, 606, 883, 1123, 2080
Bsp19I	CCATGG	2 cut(s)	1243, 1681
BspACI	CCGC	3 cut(s)	1388, 1697, 1715
BspANI	GGCC	8 cut(s)	368, 954, 960, 1034, 1421, 1443, 1529, 2062
BspCNI	CTCAG	6 cut(s)	77, 298, 342, 826, 1336, 1480
BspLI	GGNNCC	6 cut(s)	204, 960, 1201, 1202, 1293, 2029
BspMI	ACCTGC	1 cut(s)	575
BspPI	GGATC	4 cut(s)	158, 412, 601, 878
BspT104I	TTCGAA	1 cut(s)	1624
BsrBI	CCGCTC	1 cut(s)	1697
BsrDI	GCAATG	2 cut(s)	273, 1869
BsrFI	RCCGGY	2 cut(s)	927, 1368
BsrGI	TGTACA	1 cut(s)	1866
BsrI	ACTGG	9 cut(s)	32, 183, 254, 1011, 1109, 1205, 1641, 1915, 2101
BssAI	RCCGGY	2 cut(s)	927, 1368
BssECI	CCNNGG	5 cut(s)	369, 955, 1243, 1681, 1705
BssMI	GATC	7 cut(s)	163, 346, 404, 606, 883, 1123, 2080
BssNI	GRCGYC	1 cut(s)	799
BssT1I	CCWWGG	4 cut(s)	369, 1243, 1681, 1705
Bst2UI	CCWGG	7 cut(s)	57, 901, 956, 1296, 1531, 1807, 2046
Bst4CI	ACNGT	6 cut(s)	28, 481, 489, 599, 949, 1150
Bst6I	CTCTTC	2 cut(s)	901, 960
BstACI	GRCGYC	1 cut(s)	799
BstAPI	GCANNNNNTGC	3 cut(s)	1238, 1640, 1655
BstAUI	TGTACA	1 cut(s)	1866
BstBI	TTCGAA	1 cut(s)	1624
BstC8I	GCNNGC	4 cut(s)	1513, 1845, 1874, 1993
BstDEI	CTNAG	6 cut(s)	64, 306, 329, 834, 1344, 1488
BstDSI	CCRYGG	2 cut(s)	1243, 1681
BstEII	GGTNACC	1 cut(s)	1103
BstENI	CCTNNNNNAGG	1 cut(s)	2022
BstF5I	GGATG	6 cut(s)	622, 981, 1168, 1453, 1955, 1987
BstHHI	GCGC	2 cut(s)	1279, 1397
BstKTI	GATC	7 cut(s)	166, 349, 407, 609, 886, 1126, 2083
BstMBI	GATC	7 cut(s)	163, 346, 404, 606, 883, 1123, 2080
BstMWI	GCNNNNNNNGC	9 cut(s)	503, 659, 1031, 1238, 1637, 1640, 1646, 1655, 1949
BstNI	CCWGG	7 cut(s)	57, 901, 956, 1296, 1531, 1807, 2046
BstPI	GGTNACC	1 cut(s)	1103
BstSCI	CCNGG	8 cut(s)	55, 899, 954, 1294, 1443, 1529, 1805, 2044
BstSLI	GKGCMC	2 cut(s)	87, 962
BstV1I	GCAGC	6 cut(s)	593, 649, 667, 670, 1627, 1643
BstX2I	RGATCY	2 cut(s)	163, 404
BstXI	CCANNNNNNTGG	1 cut(s)	1682
BstYI	RGATCY	2 cut(s)	163, 404
Bsu36I	CCTNAGG	1 cut(s)	834
BsuRI	GGCC	8 cut(s)	368, 954, 960, 1034, 1421, 1443, 1529, 2062
BtgI	CCRYGG	2 cut(s)	1243, 1681
BtsCI	GGATG	6 cut(s)	622, 981, 1168, 1453, 1955, 1987
BtsI	GCAGTG	1 cut(s)	1397
BtsIMutI	CAGTG	7 cut(s)	20, 477, 1018, 1341, 1397, 1634, 2094
BveI	ACCTGC	1 cut(s)	575
Cac8I	GCNNGC	4 cut(s)	1513, 1845, 1874, 1993
CaiI	CAGNNNCTG	3 cut(s)	198, 1115, 1319
CfoI	GCGC	2 cut(s)	1279, 1397
Cfr10I	RCCGGY	2 cut(s)	927, 1368
CseI	GACGC	1 cut(s)	1613
CsiI	ACCWGGT	1 cut(s)	1294
Csp6I	GTAC	2 cut(s)	1668, 1867
CspCI	CAANNNNNGTGG	2 cut(s)	463, 498
CviAII	CATG	9 cut(s)	707, 790, 1217, 1244, 1433, 1676, 1682, 1903, 2032
CviQI	GTAC	2 cut(s)	1668, 1867
DdeI	CTNAG	6 cut(s)	64, 306, 329, 834, 1344, 1488
DpnI	GATC	7 cut(s)	165, 348, 406, 608, 885, 1125, 2082
DpnII	GATC	7 cut(s)	163, 346, 404, 606, 883, 1123, 2080
EaeI	YGGCCR	2 cut(s)	952, 1527
Eam1104I	CTCTTC	2 cut(s)	901, 960
EarI	CTCTTC	2 cut(s)	901, 960
Eco130I	CCWWGG	4 cut(s)	369, 1243, 1681, 1705
Eco147I	AGGCCT	1 cut(s)	2062
Eco24I	GRGCYC	3 cut(s)	207, 218, 962
Eco32I	GATATC	1 cut(s)	787
Eco47I	GGWCC	5 cut(s)	554, 838, 1200, 1223, 1292
Eco57I	CTGAAG	2 cut(s)	984, 1759
Eco81I	CCTNAGG	1 cut(s)	834
Eco91I	GGTNACC	1 cut(s)	1103
EcoNI	CCTNNNNNAGG	1 cut(s)	2022
EcoO109I	RGGNCCY	5 cut(s)	838, 958, 959, 1200, 1419
EcoO65I	GGTNACC	1 cut(s)	1103
EcoRII	CCWGG	7 cut(s)	55, 899, 954, 1294, 1529, 1805, 2044
EcoRV	GATATC	1 cut(s)	787
EcoT14I	CCWWGG	4 cut(s)	369, 1243, 1681, 1705
EcoT22I	ATGCAT	2 cut(s)	1434, 1904
EcoT38I	GRGCYC	3 cut(s)	207, 218, 962
ErhI	CCWWGG	4 cut(s)	369, 1243, 1681, 1705
FaeI	CATG	9 cut(s)	710, 793, 1220, 1247, 1436, 1679, 1685, 1906, 2035
FalI	AAGNNNNNCTT	2 cut(s)	97, 129
FaqI	GGGAC	4 cut(s)	14, 242, 1213, 1305
FatI	CATG	9 cut(s)	706, 789, 1216, 1243, 1432, 1675, 1681, 1902, 2031
FauNDI	CATATG	2 cut(s)	1651, 1813
FblI	GTMKAC	2 cut(s)	470, 1701
Fnu4HI	GCNGC	6 cut(s)	582, 663, 681, 684, 1632, 1641
FokI	GGATG	6 cut(s)	629, 988, 1175, 1460, 1942, 1994
FriOI	GRGCYC	3 cut(s)	207, 218, 962
Fsp4HI	GCNGC	6 cut(s)	582, 663, 681, 684, 1632, 1641
FspBI	CTAG	7 cut(s)	231, 408, 677, 870, 1049, 1706, 2058
FspI	TGCGCA	1 cut(s)	1396
GlaI	GCGC	2 cut(s)	1278, 1396
GluI	GCNGC	6 cut(s)	582, 663, 681, 684, 1632, 1641
GsaI	CCCAGC	4 cut(s)	263, 815, 1461, 1647
GsuI	CTGGAG	1 cut(s)	922
HaeIII	GGCC	8 cut(s)	368, 954, 960, 1034, 1421, 1443, 1529, 2062
HapII	CCGG	3 cut(s)	928, 1369, 1445
HgaI	GACGC	1 cut(s)	1613
HhaI	GCGC	2 cut(s)	1279, 1397
Hin1I	GRCGYC	1 cut(s)	799
Hin1II	CATG	9 cut(s)	710, 793, 1220, 1247, 1436, 1679, 1685, 1906, 2035
Hin6I	GCGC	2 cut(s)	1277, 1395
HinP1I	GCGC	2 cut(s)	1277, 1395
HincII	GTYRAC	2 cut(s)	134, 542
HindII	GTYRAC	2 cut(s)	134, 542
HindIII	AAGCTT	2 cut(s)	495, 1725
HinfI	GANTC	6 cut(s)	6, 60, 195, 890, 1189, 1598
HpaII	CCGG	3 cut(s)	928, 1369, 1445
HphI	GGTGA	6 cut(s)	12, 243, 889, 991, 1115, 1664
Hpy166II	GTNNAC	7 cut(s)	20, 134, 298, 471, 542, 1582, 1702
Hpy188I	TCNGA	7 cut(s)	307, 724, 1136, 1603, 1722, 1840, 1930
Hpy188III	TCNNGA	6 cut(s)	335, 512, 887, 1193, 2084, 2118
Hpy8I	GTNNAC	7 cut(s)	20, 134, 298, 471, 542, 1582, 1702
HpyAV	CCTTC	6 cut(s)	455, 514, 545, 758, 1153, 2094
HpyCH4III	ACNGT	6 cut(s)	28, 481, 489, 599, 949, 1150
HpyCH4IV	ACGT	1 cut(s)	799
HpyF10VI	GCNNNNNNNGC	9 cut(s)	503, 659, 1031, 1238, 1637, 1640, 1646, 1655, 1949
HpyF3I	CTNAG	6 cut(s)	64, 306, 329, 834, 1344, 1488
HpySE526I	ACGT	1 cut(s)	799
Hsp92I	GRCGYC	1 cut(s)	799
Hsp92II	CATG	9 cut(s)	710, 793, 1220, 1247, 1436, 1679, 1685, 1906, 2035
HspAI	GCGC	2 cut(s)	1277, 1395
KflI	GGGWCCC	1 cut(s)	1200
Kzo9I	GATC	7 cut(s)	163, 346, 404, 606, 883, 1123, 2080
LmnI	GCTCC	5 cut(s)	202, 1694, 1840, 1949, 2000
Lsp1109I	GCAGC	6 cut(s)	593, 649, 667, 670, 1627, 1643
LweI	GCATC	2 cut(s)	1153, 1645
MabI	ACCWGGT	1 cut(s)	1294
MaeI	CTAG	7 cut(s)	231, 408, 677, 870, 1049, 1706, 2058
MaeII	ACGT	1 cut(s)	799
MaeIII	GTNAC	6 cut(s)	526, 593, 1103, 1111, 1309, 1738
MalI	GATC	7 cut(s)	165, 348, 406, 608, 885, 1125, 2082
MbiI	CCGCTC	1 cut(s)	1697
MboI	GATC	7 cut(s)	163, 346, 404, 606, 883, 1123, 2080
MboII	GAAGA	5 cut(s)	93, 665, 863, 918, 977
MflI	RGATCY	2 cut(s)	163, 404
MhlI	GDGCHC	6 cut(s)	87, 207, 218, 962, 1954, 2039
MlsI	TGGCCA	2 cut(s)	954, 1529
MluNI	TGGCCA	2 cut(s)	954, 1529
MlyI	GAGTC	2 cut(s)	1183, 1592
MmeI	TCCRAC	4 cut(s)	219, 463, 1114, 2078
Mox20I	TGGCCA	2 cut(s)	954, 1529
Mph1103I	ATGCAT	2 cut(s)	1434, 1904
MroXI	GAANNNNTTC	1 cut(s)	1586
MscI	TGGCCA	2 cut(s)	954, 1529
MslI	CAYNNNNRTG	3 cut(s)	1390, 1437, 1680
Msp20I	TGGCCA	2 cut(s)	954, 1529
MspA1I	CMGCKG	2 cut(s)	263, 662
MspI	CCGG	3 cut(s)	928, 1369, 1445
MspR9I	CCNGG	8 cut(s)	57, 901, 956, 1296, 1445, 1531, 1807, 2046
Mva1269I	GAATGC	1 cut(s)	1621
MvaI	CCWGG	7 cut(s)	57, 901, 956, 1296, 1531, 1807, 2046
MwoI	GCNNNNNNNGC	9 cut(s)	503, 659, 1031, 1238, 1637, 1640, 1646, 1655, 1949
NciI	CCSGG	1 cut(s)	1445
NcoI	CCATGG	2 cut(s)	1243, 1681
NdeI	CATATG	2 cut(s)	1651, 1813
NdeII	GATC	7 cut(s)	163, 346, 404, 606, 883, 1123, 2080
NlaIII	CATG	9 cut(s)	710, 793, 1220, 1247, 1436, 1679, 1685, 1906, 2035
NlaIV	GGNNCC	6 cut(s)	204, 960, 1201, 1202, 1293, 2029
NmuCI	GTSAC	3 cut(s)	526, 1103, 1309
NsbI	TGCGCA	1 cut(s)	1396
NsiI	ATGCAT	2 cut(s)	1434, 1904
NspV	TTCGAA	1 cut(s)	1624
OliI	CACNNNNGTG	1 cut(s)	1390
PaqCI	CACCTGC	1 cut(s)	575
PceI	AGGCCT	1 cut(s)	2062
PctI	GAATGC	1 cut(s)	1621
PdmI	GAANNNNTTC	1 cut(s)	1586
PfeI	GAWTC	3 cut(s)	60, 195, 890
PflFI	GACNNNGTC	1 cut(s)	489
PflMI	CCANNNNNTGG	1 cut(s)	563
PfoI	TCCNGGA	1 cut(s)	55
PkrI	GCNGC	6 cut(s)	583, 664, 682, 685, 1633, 1642
PleI	GAGTC	2 cut(s)	1183, 1592
PpsI	GAGTC	2 cut(s)	1183, 1592
PpuMI	RGGWCCY	2 cut(s)	838, 1200
PshBI	ATTAAT	1 cut(s)	1181
Psp5II	RGGWCCY	2 cut(s)	838, 1200
Psp6I	CCWGG	7 cut(s)	55, 899, 954, 1294, 1529, 1805, 2044
PspEI	GGTNACC	1 cut(s)	1103
PspFI	CCCAGC	4 cut(s)	259, 811, 1457, 1643
PspGI	CCWGG	7 cut(s)	55, 899, 954, 1294, 1529, 1805, 2044
PspN4I	GGNNCC	6 cut(s)	204, 960, 1201, 1202, 1293, 2029
PspOMI	GGGCCC	1 cut(s)	958
PspPPI	RGGWCCY	2 cut(s)	838, 1200
PstNI	CAGNNNCTG	3 cut(s)	198, 1115, 1319
PsuI	RGATCY	2 cut(s)	163, 404
PsyI	GACNNNGTC	1 cut(s)	489
PvuII	CAGCTG	2 cut(s)	263, 662
RsaI	GTAC	2 cut(s)	1669, 1868
RsaNI	GTAC	2 cut(s)	1668, 1867
RseI	CAYNNNNRTG	3 cut(s)	1390, 1437, 1680
SatI	GCNGC	6 cut(s)	582, 663, 681, 684, 1632, 1641
Sau3AI	GATC	7 cut(s)	163, 346, 404, 606, 883, 1123, 2080
SchI	GAGTC	2 cut(s)	1183, 1592
ScrFI	CCNGG	8 cut(s)	57, 901, 956, 1296, 1445, 1531, 1807, 2046
SduI	GDGCHC	6 cut(s)	87, 207, 218, 962, 1954, 2039
SexAI	ACCWGGT	1 cut(s)	1294
SfaNI	GCATC	2 cut(s)	1153, 1645
SfuI	TTCGAA	1 cut(s)	1624
SinI	GGWCC	5 cut(s)	554, 838, 1200, 1223, 1292
SmiMI	CAYNNNNRTG	3 cut(s)	1390, 1437, 1680
SmlI	CTYRAG	1 cut(s)	1193
SmoI	CTYRAG	1 cut(s)	1193
SseBI	AGGCCT	1 cut(s)	2062
SsiI	CCGC	3 cut(s)	1388, 1697, 1715
SspI	AATATT	1 cut(s)	1237
SspMI	CTAG	7 cut(s)	231, 408, 677, 870, 1049, 1706, 2058
StuI	AGGCCT	1 cut(s)	2062
StyD4I	CCNGG	8 cut(s)	55, 899, 954, 1294, 1443, 1529, 1805, 2044
StyI	CCWWGG	4 cut(s)	369, 1243, 1681, 1705
TaaI	ACNGT	6 cut(s)	28, 481, 489, 599, 949, 1150
TaiI	ACGT	1 cut(s)	802
TaqI	TCGA	3 cut(s)	69, 697, 1624
TatI	WGTACW	2 cut(s)	1667, 1866
TfiI	GAWTC	3 cut(s)	60, 195, 890
TscAI	CASTG	7 cut(s)	20, 484, 1018, 1348, 1397, 1641, 2101
TseFI	GTSAC	3 cut(s)	526, 1103, 1309
TseI	GCWGC	6 cut(s)	581, 662, 680, 683, 1631, 1640
Tsp45I	GTSAC	3 cut(s)	526, 1103, 1309
TspDTI	ATGAA	7 cut(s)	408, 723, 752, 1172, 1205, 1370, 1911
TspRI	CASTG	7 cut(s)	20, 484, 1018, 1348, 1397, 1641, 2101
Tth111I	GACNNNGTC	1 cut(s)	489
Van91I	CCANNNNNTGG	1 cut(s)	563
VpaK11BI	GGWCC	5 cut(s)	554, 838, 1200, 1223, 1292
VspI	ATTAAT	1 cut(s)	1181
XagI	CCTNNNNNAGG	1 cut(s)	2022
XapI	RAATTY	5 cut(s)	863, 1249, 1349, 1522, 1586
XcmI	CCANNNNNNNNNTGG	3 cut(s)	235, 1018, 1682
XmaJI	CCTAGG	1 cut(s)	1705
XmiI	GTMKAC	2 cut(s)	470, 1701
XmnI	GAANNNNTTC	1 cut(s)	1586
XspI	CTAG	7 cut(s)	231, 408, 677, 870, 1049, 1706, 2058
ZraI	GACGTC	1 cut(s)	800
Zsp2I	ATGCAT	2 cut(s)	1434, 1904

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.