Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000001

Orthogroup ID

OG0005676

Physical Location & Seq

Genomic Coordinates Forward (+)

2724051 .. 2728792

Gene Structure 4742 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_1G00002300.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1944 bp

ATGAGCCCATCAAAGACCACTAGGAAGTTGGAGGTGGCTTCACCAGTCCCAGCTGACATAGACATTGCCAATTCAGTTGAGCCTGTTCACATCTCTGAGATTGCCAAAGACCTTAATCTCAGTCCTGACCACTATGATCTCTATGGAAAATACAAGGCCAAGTTTGAATCTTTAAACTGGGTTTTGAGTTTAATGAAGTGTTCTGGGGATTTGATAATGGTTTTGTTATCTGTGATTGATGAACTGAAAGGATCTAGTGATGGGTATTATGTTGTTGTGGGAGGGATTACTCCTACACCTCTTGGAGAAGGCAAGTCTACCACCACTGTTGGACTGTGTCAAGCTTTGGGTGCTTTTCAAGATAAGAAGGTTGTCACTTGCCTTCGTCAACCATCACAAGGACCAACTTTTGGAATTAAAGGTGGTGCAGCAGGTGGTGGTTACAGTCAAGTGATCCCCATGGATGAGTTTAATCTGCACTTAACAGGAGATATTCATGCAATAACAGCTGCAAACAATCTTCTAGCTGCTGCCATTGATACTCGAATGTTCCATGAAGCAACCCAATCAGATAAGGCTCTAATGAACCGATTATGCCCACCAAACAAAGAAGGAAAAAAGAGCTTTAATGATATCATGTTTAGACGTCTAAAGAAGCTGGGTATCTCCAAGACACAGCCTGAGGACCTTACACCAGAAGAAGTTAAGAAATTTGCTAGGCTTGATATTGATCCAGATTCTATCACCTGGAGAAGAGTAATGGATATAAATGACCGGCTGTTGAGAAAAATAACAGTTGGCCAGGGCCCTGAAGAGAAAGGGATGGTGAGAGAAACAGGATTTGATATTTCAGTTGCCAGTGAGATAATGGCAGTTTTGGCCCTTACTACATCTCTAGCAGATATGAGGGAGAGGCTTGGCAAAATGGTAATCGGAAACAGCAGGGCTGGTGACCCAGTTACTGCCGACGATCTTGGTGTCGGAGGTGCATTAACGGTTCTAATGAAGGATGCTATTAACCCTACATTAATGCAGACTCTTGAGGGGACTCCAGTTCTTGTTCATGCTGGTCCTTTTGCAAATATTGCGCATGGAAATTCCTCCATTGTGGCTGATAAGATTGCGCTAAAGCTGGTGGGACCAGGTGGGTTTGTAGTCACAGAAGCTGGTTTTGGTGCTGATATTGGCACTGAGAAATTCATGAATATAAAATGCCGGTATAGTGGTTTAACACCGCAGTGTGCAATTATAGTTGCCACCATAAGGGCCTTGAAAATGCATGGTGGTGGCCCGGATGTTGTTGCTGGGAAGCCTCTTAATAGTGCCTACATGACTGAGAATGTGGCTCTGGTTGAGGCTGGCTGTGTGAATTTGGCCAGGCATATTACAAATACAAAAGCCTATGGTGTGAATGTTGTTGTTGCCGTGAACAAGTTCTCAACTGACTCTGACGCAGAATTAAATGCAGTTCGAAATGCAGCACTGGCTGCTGGGGCGTATGATGCTGTGATATGTACTCACCATGCCCATGGTGGAAAAGGAGCGGTAGACCTAGGGATTGCGGTTCAGAAAGCCTGTGAGAATGTTACACAACCATTAAAGTTTTTATATCCTTTGGAATTCAGTATTAAAGAAAAGATAGAGGCAATAGCCAGTTCAATATCTGCAGCTGAAAAGCAGATTGCAATGTACAGCAGGCAAGGGTTTTCTGGTCTGCCAATATGTATGGCAAAGACCCAGTATTCATTTTCTGATAAAGCTACTGCGAAAGGAGCACCATCCAATTTTGTCTTACCAATAAGGGATGTGAGGGCAAGCATTGGAGCTGGATTTATATATCCTTTGGTAGGAACCATGAGCACAATGCCAGGACTTCCAACTAGGCCTTGCTTTTATGATATTGATCTTGACACCACCACTGGAAGGGTTATTGGTCTTTCTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004329 GO:0004477 GO:0004488 GO:0005488 GO:0005507 GO:0005575 GO:0005576 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005886 GO:0006082 GO:0006139 GO:0006144 GO:0006575 GO:0006725 GO:0006732 GO:0006760 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009108 GO:0009112 GO:0009113 GO:0009256 GO:0009257 GO:0009396 GO:0009507 GO:0009536 GO:0009719 GO:0009725 GO:0009735 GO:0009987 GO:0010033 GO:0010035 GO:0010038 GO:0016020 GO:0016053 GO:0016491 GO:0016645 GO:0016646 GO:0016787 GO:0016810 GO:0016814 GO:0016874 GO:0016879 GO:0018130 GO:0019238 GO:0019438 GO:0019752 GO:0034641 GO:0042221 GO:0042398 GO:0042440 GO:0042558 GO:0042559 GO:0043167 GO:0043169 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0043603 GO:0043604 GO:0043648 GO:0043650 GO:0044237 GO:0044238 GO:0044249 GO:0044271 GO:0044281 GO:0044283 GO:0044424 GO:0044444 GO:0044464 GO:0046112 GO:0046148 GO:0046394 GO:0046483 GO:0046653 GO:0046654 GO:0046686 GO:0046872 GO:0046914 GO:0048046 GO:0050896 GO:0051186 GO:0051188 GO:0055086 GO:0055114 GO:0071704 GO:0071944 GO:0072521 GO:0072522 GO:1901360 GO:1901362 GO:1901564 GO:1901566 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00670 ko00720 ko01100 ko01120 ko01200 map00670 map00720 map01100 map01120 map01200

Pfam Domains

Protein Families

FTHFS

Protein Analysis

647

Amino Acids

68.87

Weight (kDa)

7.16

Isoelectric Point (pI)

32.76

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
FTHFS	PF01268	18 - 647	1.7e-237	Formate--tetrahydrofolate ligase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0005676)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G50480
fragaria_vesca	FvH4_3g44470 FvH4_3g44470 FvH4_3g44470 FvH4_3g44470 FvH4_3g44470 FvH4_3g44470 FvH4_3g44470
malus_domestica	MD03G1012400.v1.1 MD11G1015100.v1.1
prunus_persica	Prupe.6G013000_v2.0.a1 Prupe.6G013000_v2.0.a1 Prupe.6G013000_v2.0.a1
pyrus_communis	pycom03g01100 pycom11g01040
rosa_chinensis	RchiOBHm_Chr5g0081161
rosa_laevigata	RLG00000036914
rosa_multiflora	Rmu_sc0016124.1_g000001 Rmu_ssc0000432.1_g000078
rosa_roxburghii	Rroxscaffold_1G00002300
rosa_rugosa	Rorug05G0473600
rosa_samantha	Rh1DG124300 Rh5AG526200 Rh5BG550500 Rh5CG573400 Rh5DG560200
rosa_wichuraiana	Rw1G009600 Rw5G048890

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	422
AatII	GACGTC	1 cut(s)	649
Acc16I	TGCGCA	1 cut(s)	1089
Acc36I	ACCTGC	1 cut(s)	422
AccB7I	CCANNNNNTGG	1 cut(s)	410
AccBSI	CCGCTC	1 cut(s)	1544
AccI	GTMKAC	2 cut(s)	317, 1548
AciI	CCGC	3 cut(s)	1235, 1544, 1562
AclWI	GGATC	3 cut(s)	259, 448, 725
AcoI	YGGCCR	2 cut(s)	799, 1374
AcsI	RAATTY	5 cut(s)	710, 1096, 1196, 1369, 1619
AcuI	CTGAAG	1 cut(s)	831
AcyI	GRCGYC	1 cut(s)	646
AfaI	GTAC	2 cut(s)	1516, 1691
AfiI	CCNNNNNNNGG	5 cut(s)	398, 410, 1263, 1847, 1923
AgsI	TTSAA	4 cut(s)	167, 359, 1273, 1659
AjnI	CCWGG	5 cut(s)	746, 801, 1141, 1376, 1867
AjuI	GAANNNNNNNTTGG	2 cut(s)	1155, 1187
AleI	CACNNNNGTG	1 cut(s)	1237
Alw21I	GWGCWC	2 cut(s)	1777, 1862
AlwI	GGATC	3 cut(s)	259, 448, 725
AlwNI	CAGNNNCTG	2 cut(s)	962, 1166
AoxI	GGCC	8 cut(s)	156, 799, 805, 879, 1266, 1288, 1374, 1883
ApaI	GGGCCC	1 cut(s)	809
ApeKI	GCWGC	7 cut(s)	428, 509, 527, 530, 1478, 1487, 1667
ApoI	RAATTY	5 cut(s)	710, 1096, 1196, 1369, 1619
AseI	ATTAAT	1 cut(s)	1028
Asp700I	GAANNNNTTC	1 cut(s)	1433
AspA2I	CCTAGG	1 cut(s)	1552
AspLEI	GCGC	2 cut(s)	1090, 1126
AspS9I	GGNCC	9 cut(s)	401, 685, 805, 806, 880, 1070, 1139, 1266, 1289
AsuC2I	CCSGG	1 cut(s)	1292
AsuHPI	GGTGA	5 cut(s)	33, 736, 838, 962, 1511
AsuII	TTCGAA	1 cut(s)	1471
AvaII	GGWCC	4 cut(s)	401, 685, 1070, 1139
AvrII	CCTAGG	1 cut(s)	1552
AxyI	CCTNAGG	1 cut(s)	681
BaeGI	GKGCMC	1 cut(s)	809
BalI	TGGCCA	2 cut(s)	801, 1376
BanII	GRGCYC	2 cut(s)	8, 809
Bbv12I	GWGCWC	2 cut(s)	1777, 1862
BbvI	GCAGC	7 cut(s)	440, 496, 514, 517, 1474, 1490, 1679
BccI	CCATC	5 cut(s)	16, 254, 400, 817, 1786
BceAI	ACGGC	1 cut(s)	1409
BciT130I	CCWGG	5 cut(s)	748, 803, 1143, 1378, 1869
BcnI	CCSGG	1 cut(s)	1292
BfaI	CTAG	7 cut(s)	21, 255, 524, 717, 896, 1553, 1881
BfmI	CTRYAG	1 cut(s)	1665
BfuAI	ACCTGC	1 cut(s)	422
BisI	GCNGC	7 cut(s)	429, 510, 528, 531, 1479, 1488, 1668
BlnI	CCTAGG	1 cut(s)	1552
BlsI	GCNGC	7 cut(s)	430, 511, 529, 532, 1480, 1489, 1669
Bme1390I	CCNGG	6 cut(s)	748, 803, 1143, 1292, 1378, 1869
Bme18I	GGWCC	4 cut(s)	401, 685, 1070, 1139
BmgT120I	GGNCC	9 cut(s)	401, 685, 805, 806, 880, 1070, 1139, 1266, 1289
BmiI	GGNNCC	3 cut(s)	807, 1140, 1852
BmrFI	CCNGG	6 cut(s)	748, 803, 1143, 1292, 1378, 1869
BmrI	ACTGGG	3 cut(s)	187, 950, 1732
BmsI	GCATC	2 cut(s)	1000, 1492
BmuI	ACTGGG	3 cut(s)	187, 950, 1732
BpmI	CTGGAG	2 cut(s)	769, 1035
Bpu14I	TTCGAA	1 cut(s)	1471
BpuEI	CTTGAG	1 cut(s)	1061
BpuMI	CCSGG	1 cut(s)	1292
BsaBI	GATNNNNATC	2 cut(s)	729, 1902
BsaHI	GRCGYC	1 cut(s)	646
BsaJI	CCNNGG	4 cut(s)	459, 802, 1528, 1552
Bsc4I	CCNNNNNNNGG	5 cut(s)	398, 410, 1263, 1847, 1923
Bse118I	RCCGGY	2 cut(s)	774, 1215
Bse1I	ACTGG	9 cut(s)	44, 182, 858, 956, 1052, 1488, 1653, 1738, 1924
Bse21I	CCTNAGG	1 cut(s)	681
Bse3DI	GCAATG	2 cut(s)	63, 1692
Bse8I	GATNNNNATC	2 cut(s)	729, 1902
BseBI	CCWGG	5 cut(s)	748, 803, 1143, 1378, 1869
BseDI	CCNNGG	4 cut(s)	459, 802, 1528, 1552
BseGI	GGATG	6 cut(s)	469, 828, 1015, 1300, 1778, 1810
BseJI	GATNNNNATC	2 cut(s)	729, 1902
BseLI	CCNNNNNNNGG	5 cut(s)	398, 410, 1263, 1847, 1923
BseMI	GCAATG	2 cut(s)	63, 1692
BseMII	CTCAG	5 cut(s)	87, 133, 672, 1182, 1326
BseNI	ACTGG	9 cut(s)	44, 182, 858, 956, 1052, 1488, 1653, 1738, 1924
BseSI	GKGCMC	1 cut(s)	809
BseXI	GCAGC	7 cut(s)	440, 496, 514, 517, 1474, 1490, 1679
BseYI	CCCAGC	4 cut(s)	49, 658, 1304, 1490
BsgI	GTGCAG	2 cut(s)	447, 461
BshFI	GGCC	8 cut(s)	158, 801, 807, 881, 1268, 1290, 1376, 1885
BsiHKAI	GWGCWC	2 cut(s)	1777, 1862
BsiSI	CCGG	3 cut(s)	775, 1216, 1292
BslFI	GGGAC	3 cut(s)	32, 1060, 1152
BslI	CCNNNNNNNGG	5 cut(s)	398, 410, 1263, 1847, 1923
BsmFI	GGGAC	3 cut(s)	32, 1060, 1152
BsnI	GGCC	8 cut(s)	158, 801, 807, 881, 1268, 1290, 1376, 1885
Bsp119I	TTCGAA	1 cut(s)	1471
Bsp120I	GGGCCC	1 cut(s)	805
Bsp1286I	GDGCHC	4 cut(s)	8, 809, 1777, 1862
Bsp1407I	TGTACA	1 cut(s)	1689
Bsp143I	GATC	6 cut(s)	136, 251, 453, 730, 970, 1903
Bsp19I	CCATGG	2 cut(s)	459, 1528
BspACI	CCGC	3 cut(s)	1235, 1544, 1562
BspANI	GGCC	8 cut(s)	158, 801, 807, 881, 1268, 1290, 1376, 1885
BspCNI	CTCAG	5 cut(s)	88, 132, 673, 1183, 1327
BspHI	TCATGA	1 cut(s)	1200
BspLI	GGNNCC	3 cut(s)	807, 1140, 1852
BspMAI	CTGCAG	1 cut(s)	1669
BspMI	ACCTGC	1 cut(s)	422
BspPI	GGATC	3 cut(s)	259, 448, 725
BspT104I	TTCGAA	1 cut(s)	1471
BsrBI	CCGCTC	1 cut(s)	1544
BsrDI	GCAATG	2 cut(s)	63, 1692
BsrFI	RCCGGY	2 cut(s)	774, 1215
BsrGI	TGTACA	1 cut(s)	1689
BsrI	ACTGG	9 cut(s)	44, 182, 858, 956, 1052, 1488, 1653, 1738, 1924
BssAI	RCCGGY	2 cut(s)	774, 1215
BssECI	CCNNGG	4 cut(s)	459, 802, 1528, 1552
BssMI	GATC	6 cut(s)	136, 251, 453, 730, 970, 1903
BssNI	GRCGYC	1 cut(s)	646
BssT1I	CCWWGG	3 cut(s)	459, 1528, 1552
Bst2UI	CCWGG	5 cut(s)	748, 803, 1143, 1378, 1869
Bst4CI	ACNGT	5 cut(s)	328, 336, 446, 796, 997
Bst6I	CTCTTC	2 cut(s)	748, 807
BstACI	GRCGYC	1 cut(s)	646
BstAPI	GCANNNNNTGC	2 cut(s)	1085, 1487
BstAUI	TGTACA	1 cut(s)	1689
BstBI	TTCGAA	1 cut(s)	1471
BstC8I	GCNNGC	3 cut(s)	1360, 1697, 1816
BstDEI	CTNAG	5 cut(s)	96, 119, 681, 1191, 1335
BstDSI	CCRYGG	2 cut(s)	459, 1528
BstEII	GGTNACC	1 cut(s)	950
BstENI	CCTNNNNNAGG	1 cut(s)	1845
BstF5I	GGATG	6 cut(s)	469, 828, 1015, 1300, 1778, 1810
BstHHI	GCGC	2 cut(s)	1090, 1126
BstKTI	GATC	6 cut(s)	139, 254, 456, 733, 973, 1906
BstMBI	GATC	6 cut(s)	136, 251, 453, 730, 970, 1903
BstMWI	GCNNNNNNNGC	9 cut(s)	350, 506, 878, 1085, 1484, 1487, 1493, 1502, 1772
BstNI	CCWGG	5 cut(s)	748, 803, 1143, 1378, 1869
BstPI	GGTNACC	1 cut(s)	950
BstSCI	CCNGG	6 cut(s)	746, 801, 1141, 1290, 1376, 1867
BstSFI	CTRYAG	1 cut(s)	1665
BstSLI	GKGCMC	1 cut(s)	809
BstV1I	GCAGC	7 cut(s)	440, 496, 514, 517, 1474, 1490, 1679
BstX2I	RGATCY	1 cut(s)	251
BstXI	CCANNNNNNTGG	1 cut(s)	1529
BstYI	RGATCY	1 cut(s)	251
Bsu36I	CCTNAGG	1 cut(s)	681
BsuRI	GGCC	8 cut(s)	158, 801, 807, 881, 1268, 1290, 1376, 1885
BtgI	CCRYGG	2 cut(s)	459, 1528
BtsCI	GGATG	6 cut(s)	469, 828, 1015, 1300, 1778, 1810
BtsI	GCAGTG	1 cut(s)	1244
BtsIMutI	CAGTG	6 cut(s)	324, 865, 1188, 1244, 1481, 1917
BveI	ACCTGC	1 cut(s)	422
Cac8I	GCNNGC	3 cut(s)	1360, 1697, 1816
CaiI	CAGNNNCTG	2 cut(s)	962, 1166
CciI	TCATGA	1 cut(s)	1200
CfoI	GCGC	2 cut(s)	1090, 1126
Cfr10I	RCCGGY	2 cut(s)	774, 1215
Cfr13I	GGNCC	9 cut(s)	401, 685, 805, 806, 880, 1070, 1139, 1266, 1289
CseI	GACGC	1 cut(s)	1460
CsiI	ACCWGGT	1 cut(s)	1141
Csp6I	GTAC	2 cut(s)	1515, 1690
CspCI	CAANNNNNGTGG	2 cut(s)	310, 345
CviQI	GTAC	2 cut(s)	1515, 1690
DdeI	CTNAG	5 cut(s)	96, 119, 681, 1191, 1335
DpnI	GATC	6 cut(s)	138, 253, 455, 732, 972, 1905
DpnII	GATC	6 cut(s)	136, 251, 453, 730, 970, 1903
DraI	TTTAAA	1 cut(s)	174
EaeI	YGGCCR	2 cut(s)	799, 1374
Eam1104I	CTCTTC	2 cut(s)	748, 807
EarI	CTCTTC	2 cut(s)	748, 807
Eco130I	CCWWGG	3 cut(s)	459, 1528, 1552
Eco147I	AGGCCT	1 cut(s)	1885
Eco24I	GRGCYC	2 cut(s)	8, 809
Eco32I	GATATC	1 cut(s)	634
Eco47I	GGWCC	4 cut(s)	401, 685, 1070, 1139
Eco57I	CTGAAG	1 cut(s)	831
Eco81I	CCTNAGG	1 cut(s)	681
Eco91I	GGTNACC	1 cut(s)	950
EcoNI	CCTNNNNNAGG	1 cut(s)	1845
EcoO109I	RGGNCCY	4 cut(s)	685, 805, 806, 1266
EcoO65I	GGTNACC	1 cut(s)	950
EcoRI	GAATTC	1 cut(s)	1619
EcoRII	CCWGG	5 cut(s)	746, 801, 1141, 1376, 1867
EcoRV	GATATC	1 cut(s)	634
EcoT14I	CCWWGG	3 cut(s)	459, 1528, 1552
EcoT22I	ATGCAT	1 cut(s)	1281
EcoT38I	GRGCYC	2 cut(s)	8, 809
ErhI	CCWWGG	3 cut(s)	459, 1528, 1552
FaqI	GGGAC	3 cut(s)	32, 1060, 1152
FblI	GTMKAC	2 cut(s)	317, 1548
Fnu4HI	GCNGC	7 cut(s)	429, 510, 528, 531, 1479, 1488, 1668
FokI	GGATG	6 cut(s)	476, 835, 1022, 1307, 1765, 1817
FriOI	GRGCYC	2 cut(s)	8, 809
Fsp4HI	GCNGC	7 cut(s)	429, 510, 528, 531, 1479, 1488, 1668
FspBI	CTAG	7 cut(s)	21, 255, 524, 717, 896, 1553, 1881
FspI	TGCGCA	1 cut(s)	1089
GlaI	GCGC	2 cut(s)	1089, 1125
GluI	GCNGC	7 cut(s)	429, 510, 528, 531, 1479, 1488, 1668
GsaI	CCCAGC	4 cut(s)	53, 662, 1308, 1494
GsuI	CTGGAG	2 cut(s)	769, 1035
HaeIII	GGCC	8 cut(s)	158, 801, 807, 881, 1268, 1290, 1376, 1885
HapII	CCGG	3 cut(s)	775, 1216, 1292
HgaI	GACGC	1 cut(s)	1460
HhaI	GCGC	2 cut(s)	1090, 1126
Hin1I	GRCGYC	1 cut(s)	646
Hin6I	GCGC	2 cut(s)	1088, 1124
HinP1I	GCGC	2 cut(s)	1088, 1124
HincII	GTYRAC	1 cut(s)	389
HindII	GTYRAC	1 cut(s)	389
HindIII	AAGCTT	1 cut(s)	342
HinfI	GANTC	5 cut(s)	167, 737, 1036, 1048, 1445
HpaII	CCGG	3 cut(s)	775, 1216, 1292
HphI	GGTGA	5 cut(s)	33, 736, 838, 962, 1511
Hpy166II	GTNNAC	5 cut(s)	88, 318, 389, 1429, 1549
Hpy188I	TCNGA	7 cut(s)	97, 571, 935, 983, 1450, 1569, 1753
Hpy188III	TCNNGA	7 cut(s)	125, 359, 734, 1040, 1201, 1907, 1941
Hpy8I	GTNNAC	5 cut(s)	88, 318, 389, 1429, 1549
Hpy99I	CGWCG	1 cut(s)	971
HpyAV	CCTTC	6 cut(s)	302, 361, 392, 605, 1000, 1917
HpyCH4III	ACNGT	5 cut(s)	328, 336, 446, 796, 997
HpyCH4IV	ACGT	1 cut(s)	646
HpyF10VI	GCNNNNNNNGC	9 cut(s)	350, 506, 878, 1085, 1484, 1487, 1493, 1502, 1772
HpyF3I	CTNAG	5 cut(s)	96, 119, 681, 1191, 1335
HpySE526I	ACGT	1 cut(s)	646
Hsp92I	GRCGYC	1 cut(s)	646
HspAI	GCGC	2 cut(s)	1088, 1124
Kzo9I	GATC	6 cut(s)	136, 251, 453, 730, 970, 1903
LmnI	GCTCC	3 cut(s)	1541, 1772, 1823
Lsp1109I	GCAGC	7 cut(s)	440, 496, 514, 517, 1474, 1490, 1679
LweI	GCATC	2 cut(s)	1000, 1492
MabI	ACCWGGT	1 cut(s)	1141
MaeI	CTAG	7 cut(s)	21, 255, 524, 717, 896, 1553, 1881
MaeII	ACGT	1 cut(s)	646
MaeIII	GTNAC	6 cut(s)	373, 440, 950, 958, 1156, 1585
MalI	GATC	6 cut(s)	138, 253, 455, 732, 972, 1905
MbiI	CCGCTC	1 cut(s)	1544
MboI	GATC	6 cut(s)	136, 251, 453, 730, 970, 1903
MboII	GAAGA	4 cut(s)	512, 710, 765, 824
MflI	RGATCY	1 cut(s)	251
MhlI	GDGCHC	4 cut(s)	8, 809, 1777, 1862
MlsI	TGGCCA	2 cut(s)	801, 1376
MluNI	TGGCCA	2 cut(s)	801, 1376
MlyI	GAGTC	3 cut(s)	1030, 1042, 1439
MmeI	TCCRAC	4 cut(s)	9, 310, 961, 1901
Mox20I	TGGCCA	2 cut(s)	801, 1376
Mph1103I	ATGCAT	1 cut(s)	1281
MroXI	GAANNNNTTC	1 cut(s)	1433
MscI	TGGCCA	2 cut(s)	801, 1376
MslI	CAYNNNNRTG	3 cut(s)	1237, 1284, 1527
Msp20I	TGGCCA	2 cut(s)	801, 1376
MspA1I	CMGCKG	3 cut(s)	53, 509, 1670
MspI	CCGG	3 cut(s)	775, 1216, 1292
MspR9I	CCNGG	6 cut(s)	748, 803, 1143, 1292, 1378, 1869
MvaI	CCWGG	5 cut(s)	748, 803, 1143, 1378, 1869
MwoI	GCNNNNNNNGC	9 cut(s)	350, 506, 878, 1085, 1484, 1487, 1493, 1502, 1772
NciI	CCSGG	1 cut(s)	1292
NcoI	CCATGG	2 cut(s)	459, 1528
NdeII	GATC	6 cut(s)	136, 251, 453, 730, 970, 1903
NlaIV	GGNNCC	3 cut(s)	807, 1140, 1852
NmuCI	GTSAC	3 cut(s)	373, 950, 1156
NsbI	TGCGCA	1 cut(s)	1089
NsiI	ATGCAT	1 cut(s)	1281
NspV	TTCGAA	1 cut(s)	1471
OliI	CACNNNNGTG	1 cut(s)	1237
PagI	TCATGA	1 cut(s)	1200
PaqCI	CACCTGC	1 cut(s)	422
PceI	AGGCCT	1 cut(s)	1885
PdmI	GAANNNNTTC	1 cut(s)	1433
PfeI	GAWTC	2 cut(s)	167, 737
PflFI	GACNNNGTC	1 cut(s)	336
PflMI	CCANNNNNTGG	1 cut(s)	410
PkrI	GCNGC	7 cut(s)	430, 511, 529, 532, 1480, 1489, 1669
PleI	GAGTC	3 cut(s)	1030, 1042, 1439
PpsI	GAGTC	3 cut(s)	1030, 1042, 1439
PpuMI	RGGWCCY	1 cut(s)	685
PshBI	ATTAAT	1 cut(s)	1028
Psp5II	RGGWCCY	1 cut(s)	685
Psp6I	CCWGG	5 cut(s)	746, 801, 1141, 1376, 1867
PspEI	GGTNACC	1 cut(s)	950
PspFI	CCCAGC	4 cut(s)	49, 658, 1304, 1490
PspGI	CCWGG	5 cut(s)	746, 801, 1141, 1376, 1867
PspN4I	GGNNCC	3 cut(s)	807, 1140, 1852
PspOMI	GGGCCC	1 cut(s)	805
PspPI	GGNCC	9 cut(s)	401, 685, 805, 806, 880, 1070, 1139, 1266, 1289
PspPPI	RGGWCCY	1 cut(s)	685
PstI	CTGCAG	1 cut(s)	1669
PstNI	CAGNNNCTG	2 cut(s)	962, 1166
PsuI	RGATCY	1 cut(s)	251
PsyI	GACNNNGTC	1 cut(s)	336
PvuII	CAGCTG	3 cut(s)	53, 509, 1670
RsaI	GTAC	2 cut(s)	1516, 1691
RsaNI	GTAC	2 cut(s)	1515, 1690
RseI	CAYNNNNRTG	3 cut(s)	1237, 1284, 1527
SatI	GCNGC	7 cut(s)	429, 510, 528, 531, 1479, 1488, 1668
Sau3AI	GATC	6 cut(s)	136, 251, 453, 730, 970, 1903
Sau96I	GGNCC	9 cut(s)	401, 685, 805, 806, 880, 1070, 1139, 1266, 1289
SchI	GAGTC	3 cut(s)	1030, 1042, 1439
ScrFI	CCNGG	6 cut(s)	748, 803, 1143, 1292, 1378, 1869
SduI	GDGCHC	4 cut(s)	8, 809, 1777, 1862
SexAI	ACCWGGT	1 cut(s)	1141
SfaNI	GCATC	2 cut(s)	1000, 1492
SfcI	CTRYAG	1 cut(s)	1665
SfuI	TTCGAA	1 cut(s)	1471
SinI	GGWCC	4 cut(s)	401, 685, 1070, 1139
SmiMI	CAYNNNNRTG	3 cut(s)	1237, 1284, 1527
SmlI	CTYRAG	1 cut(s)	1040
SmoI	CTYRAG	1 cut(s)	1040
SseBI	AGGCCT	1 cut(s)	1885
SsiI	CCGC	3 cut(s)	1235, 1544, 1562
SspI	AATATT	1 cut(s)	1084
SspMI	CTAG	7 cut(s)	21, 255, 524, 717, 896, 1553, 1881
StuI	AGGCCT	1 cut(s)	1885
StyD4I	CCNGG	6 cut(s)	746, 801, 1141, 1290, 1376, 1867
StyI	CCWWGG	3 cut(s)	459, 1528, 1552
TaaI	ACNGT	5 cut(s)	328, 336, 446, 796, 997
TaiI	ACGT	1 cut(s)	649
TaqI	TCGA	2 cut(s)	544, 1471
TatI	WGTACW	2 cut(s)	1514, 1689
TfiI	GAWTC	2 cut(s)	167, 737
TscAI	CASTG	6 cut(s)	331, 865, 1195, 1244, 1488, 1924
TseFI	GTSAC	3 cut(s)	373, 950, 1156
TseI	GCWGC	7 cut(s)	428, 509, 527, 530, 1478, 1487, 1667
Tsp45I	GTSAC	3 cut(s)	373, 950, 1156
TspRI	CASTG	6 cut(s)	331, 865, 1195, 1244, 1488, 1924
Tth111I	GACNNNGTC	1 cut(s)	336
Van91I	CCANNNNNTGG	1 cut(s)	410
VpaK11BI	GGWCC	4 cut(s)	401, 685, 1070, 1139
VspI	ATTAAT	1 cut(s)	1028
XagI	CCTNNNNNAGG	1 cut(s)	1845
XapI	RAATTY	5 cut(s)	710, 1096, 1196, 1369, 1619
XcmI	CCANNNNNNNNNTGG	3 cut(s)	25, 865, 1529
XmaJI	CCTAGG	1 cut(s)	1552
XmiI	GTMKAC	2 cut(s)	317, 1548
XmnI	GAANNNNTTC	1 cut(s)	1433
XspI	CTAG	7 cut(s)	21, 255, 524, 717, 896, 1553, 1881
ZraI	GACGTC	1 cut(s)	647
Zsp2I	ATGCAT	1 cut(s)	1281

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.