Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr5C

Orthogroup ID

OG0005676

Physical Location & Seq

Genomic Coordinates Reverse (-)

81210172 .. 81215334

Gene Structure 5163 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh5CG573400.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1731 bp

ATGCGACTCTATCAGTGTTCACCAACTGTCCCAGTTAGAGCTTTAGCCTCCAATCCTGGAATCTCAGTCGATAAGAAATCAGGGCACTCCATTCTATGTTCTTCGAGCCTCACTTTCCCATCATCAATAGGTCAACCTCAACAAACAGAACCACTTAAAACAGATCCATCAATCCCAGAAACCAGTTTTCACAGATTCTGGGAGCCCAAAATGAGCCCATCAAAGACCACTAGGAAGTTGGAGGTGGCTTCACCAGTCCCAGCTGACATAGACATTGCCAATTCAGTTGAGCCTGTTCACATCTCTGAGATTGCCAAAGAACTTAATCTCAGTCCTGACCACTATGATCTCTATGGAAAATACAAGGCCAAGGTTTTGTTATCTGTGATTGATGAACTGAAAGGATCTAGTGATGGGTATTATGTTGTTGTGGGAGGGATTACTCCTACACCTCTTGGAGAAGGCAAGTCTACCACCACTGTTGGACTGTGTCAAGCTTTGGGTGCTTTTCAAGATAAGAAGGTTGTCACTTGCCTTCGTCAACCATCACAAGGACCAACTTTTGGAATTAAAGGTGGTGCAGCAGGTGGTGGTTACAGTCAAGTGATCCCAATGGATGAGTTTAATCTGCACTTAACAGGAGATATTCATGCAATAACAGCTGCAAACAATCTTCTAGCTGCTGCCATTGATACTCGAATGTTCCATGAAGCAACCCAATCAGATAAGGCTCTAATGAACCGATTATGCCCACCAAACAAAGAAGGAAAAAAGAGCTTTAATGATATCATGTTTAGACGTCTAAAGAAGCTGGGTATCTCCAAGACACAGCCTGAGGACCTTACACCAGAAGAAGTTAAGAAATTTGCTAGGCTTGATATTGATCCAGATTCTATCACCTGGAGAAGAGTAATGGATATAAATGACCGGCTGTTGAGAAAAATAACAGTTGGCCAGGGCCCTGAAGAGAAAGGGATGGTGAGAGAAACAGGATTTGATATTTCAGTTGCCAGTGAGATAATGGCAGTTTTGGCCCTTACTACATCTCTAGCAGATATGAGGGAGAGGCTTGGCAAAATGGTAATCGGAAACAGCAGGGCTGGTGACCCAGTTACTGCCGACGATCTTGGTGTCGGAGGTGCATTAACGGTTCTAATGAAGGATGCTATTAACCCTACATTAATGCAGACTCTTGAGGGAACCCCAGTTCTTGTTCATGCTGGTCCTTTTGCAAATATTGCGCATGGAAATTCCTCCATTGTGGCTGATAAGATTGCGCTAAAGCTGGTGGGACCAGGTGGGTTTGTAGTCACAGAAGCTGGTTTTGGTGCTGATATTGGCACTGAGAAATTCATGAATATAAAATGCCGGTATAGTGGTTTAACACCGCAGTGCGCAATTATAGTTGCCACCATAAGGGCCTTGAAAATGCATGGTGGTGGCCCGGATGTTGTTGCTGGGAAGCCTCTTAATAGTGCCTACATAACTGAGAATGTGGCTCTGGTTGAGGCTGGCTGTGTGAATTTGGCCAGGCATATTACAAATACAAAAGCCTATGGTGTGAATGTTGTTGTTGCTGTGAACAAGTTCTCAACTGACTCTGACGCAGAATTGAATGCAGTTCGAAATGCAGCACTGGCTGCTGGGGCGTATGATGCTGTGATATGTACTCACCATGCCCATGGTGGAAAAGGAGCGGTTAGTCACTTTAACTTGAATTTGGTTGACTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004329 GO:0004477 GO:0004488 GO:0005488 GO:0005507 GO:0005575 GO:0005576 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005886 GO:0006082 GO:0006139 GO:0006144 GO:0006575 GO:0006725 GO:0006732 GO:0006760 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009108 GO:0009112 GO:0009113 GO:0009256 GO:0009257 GO:0009396 GO:0009507 GO:0009536 GO:0009719 GO:0009725 GO:0009735 GO:0009987 GO:0010033 GO:0010035 GO:0010038 GO:0016020 GO:0016053 GO:0016491 GO:0016645 GO:0016646 GO:0016787 GO:0016810 GO:0016814 GO:0016874 GO:0016879 GO:0018130 GO:0019238 GO:0019438 GO:0019752 GO:0034641 GO:0042221 GO:0042398 GO:0042440 GO:0042558 GO:0042559 GO:0043167 GO:0043169 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0043603 GO:0043604 GO:0043648 GO:0043650 GO:0044237 GO:0044238 GO:0044249 GO:0044271 GO:0044281 GO:0044283 GO:0044424 GO:0044444 GO:0044464 GO:0046112 GO:0046148 GO:0046394 GO:0046483 GO:0046653 GO:0046654 GO:0046686 GO:0046872 GO:0046914 GO:0048046 GO:0050896 GO:0051186 GO:0051188 GO:0055086 GO:0055114 GO:0071704 GO:0071944 GO:0072521 GO:0072522 GO:1901360 GO:1901362 GO:1901564 GO:1901566 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00670 ko00720 ko01100 ko01120 ko01200 map00670 map00720 map01100 map01120 map01200

Pfam Domains

Protein Families

FTHFS

Protein Analysis

576

Amino Acids

61.32

Weight (kDa)

7.23

Isoelectric Point (pI)

33.73

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
FTHFS	PF01268	88 - 567	1.5e-196	Formate--tetrahydrofolate ligase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Publication-ready

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Orthologous Genes (Group: OG0005676)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G50480
fragaria_vesca	FvH4_3g44470 FvH4_3g44470 FvH4_3g44470 FvH4_3g44470 FvH4_3g44470 FvH4_3g44470 FvH4_3g44470
malus_domestica	MD03G1012400.v1.1 MD11G1015100.v1.1
prunus_persica	Prupe.6G013000_v2.0.a1 Prupe.6G013000_v2.0.a1 Prupe.6G013000_v2.0.a1
pyrus_communis	pycom03g01100 pycom11g01040
rosa_chinensis	RchiOBHm_Chr5g0081161
rosa_laevigata	RLG00000036914
rosa_multiflora	Rmu_sc0016124.1_g000001 Rmu_ssc0000432.1_g000078
rosa_roxburghii	Rroxscaffold_1G00002300
rosa_rugosa	Rorug05G0473600
rosa_samantha	Rh1DG124300 Rh5AG526200 Rh5BG550500 Rh5CG573400 Rh5DG560200
rosa_wichuraiana	Rw1G009600 Rw5G048890

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	575
AatII	GACGTC	1 cut(s)	802
Acc16I	TGCGCA	2 cut(s)	1242, 1396
Acc36I	ACCTGC	1 cut(s)	575
AccB7I	CCANNNNNTGG	1 cut(s)	563
AccBSI	CCGCTC	1 cut(s)	1697
AccI	GTMKAC	1 cut(s)	470
AciI	CCGC	2 cut(s)	1388, 1697
AclWI	GGATC	4 cut(s)	158, 412, 601, 878
AcoI	YGGCCR	2 cut(s)	952, 1527
AcsI	RAATTY	5 cut(s)	863, 1249, 1349, 1522, 1717
AcuI	CTGAAG	1 cut(s)	984
AcyI	GRCGYC	1 cut(s)	799
AfaI	GTAC	1 cut(s)	1669
AfiI	CCNNNNNNNGG	3 cut(s)	551, 563, 1416
AgsI	TTSAA	4 cut(s)	512, 1426, 1615, 1717
AjnI	CCWGG	5 cut(s)	55, 899, 954, 1294, 1529
AjuI	GAANNNNNNNTTGG	2 cut(s)	1308, 1340
AleI	CACNNNNGTG	1 cut(s)	1390
AloI	GAACNNNNNNTCC	2 cut(s)	1191, 1223
AluBI	AGCT	9 cut(s)	41, 263, 497, 662, 680, 777, 811, 1285, 1319
AluI	AGCT	9 cut(s)	41, 263, 497, 662, 680, 777, 811, 1285, 1319
AlwI	GGATC	4 cut(s)	158, 412, 601, 878
AlwNI	CAGNNNCTG	3 cut(s)	198, 1115, 1319
AoxI	GGCC	7 cut(s)	366, 952, 958, 1032, 1419, 1441, 1527
ApaI	GGGCCC	1 cut(s)	962
ApeKI	GCWGC	6 cut(s)	581, 662, 680, 683, 1631, 1640
ApoI	RAATTY	5 cut(s)	863, 1249, 1349, 1522, 1717
AseI	ATTAAT	1 cut(s)	1181
Asp700I	GAANNNNTTC	1 cut(s)	1586
AspLEI	GCGC	3 cut(s)	1243, 1279, 1397
AspS9I	GGNCC	9 cut(s)	554, 838, 958, 959, 1033, 1223, 1292, 1419, 1442
AsuC2I	CCSGG	1 cut(s)	1445
AsuHPI	GGTGA	6 cut(s)	12, 243, 889, 991, 1115, 1664
AsuII	TTCGAA	1 cut(s)	1624
AvaII	GGWCC	4 cut(s)	554, 838, 1223, 1292
AxyI	CCTNAGG	1 cut(s)	834
BaeGI	GKGCMC	2 cut(s)	87, 962
BalI	TGGCCA	2 cut(s)	954, 1529
BanII	GRGCYC	3 cut(s)	207, 218, 962
BbvI	GCAGC	6 cut(s)	593, 649, 667, 670, 1627, 1643
BccI	CCATC	6 cut(s)	127, 175, 226, 407, 553, 970
BciT130I	CCWGG	5 cut(s)	57, 901, 956, 1296, 1531
BcnI	CCSGG	1 cut(s)	1445
BfaI	CTAG	5 cut(s)	231, 408, 677, 870, 1049
BfuAI	ACCTGC	1 cut(s)	575
BisI	GCNGC	6 cut(s)	582, 663, 681, 684, 1632, 1641
BlsI	GCNGC	6 cut(s)	583, 664, 682, 685, 1633, 1642
Bme1390I	CCNGG	6 cut(s)	57, 901, 956, 1296, 1445, 1531
Bme18I	GGWCC	4 cut(s)	554, 838, 1223, 1292
BmgT120I	GGNCC	9 cut(s)	554, 838, 958, 959, 1033, 1223, 1292, 1419, 1442
BmiI	GGNNCC	4 cut(s)	204, 960, 1201, 1293
BmrFI	CCNGG	6 cut(s)	57, 901, 956, 1296, 1445, 1531
BmrI	ACTGGG	3 cut(s)	26, 1103, 1199
BmsI	GCATC	2 cut(s)	1153, 1645
BmuI	ACTGGG	3 cut(s)	26, 1103, 1199
BpmI	CTGGAG	1 cut(s)	922
Bpu14I	TTCGAA	1 cut(s)	1624
BpuEI	CTTGAG	1 cut(s)	1214
BpuMI	CCSGG	1 cut(s)	1445
BsaBI	GATNNNNATC	1 cut(s)	882
BsaHI	GRCGYC	1 cut(s)	799
BsaJI	CCNNGG	3 cut(s)	369, 955, 1681
Bsc4I	CCNNNNNNNGG	3 cut(s)	551, 563, 1416
Bse118I	RCCGGY	2 cut(s)	927, 1368
Bse1I	ACTGG	7 cut(s)	32, 183, 254, 1011, 1109, 1205, 1641
Bse21I	CCTNAGG	1 cut(s)	834
Bse3DI	GCAATG	1 cut(s)	273
Bse8I	GATNNNNATC	1 cut(s)	882
BseBI	CCWGG	5 cut(s)	57, 901, 956, 1296, 1531
BseDI	CCNNGG	3 cut(s)	369, 955, 1681
BseGI	GGATG	4 cut(s)	622, 981, 1168, 1453
BseJI	GATNNNNATC	1 cut(s)	882
BseLI	CCNNNNNNNGG	3 cut(s)	551, 563, 1416
BseMI	GCAATG	1 cut(s)	273
BseMII	CTCAG	6 cut(s)	78, 297, 343, 825, 1335, 1479
BseNI	ACTGG	7 cut(s)	32, 183, 254, 1011, 1109, 1205, 1641
BseSI	GKGCMC	2 cut(s)	87, 962
BseXI	GCAGC	6 cut(s)	593, 649, 667, 670, 1627, 1643
BseYI	CCCAGC	4 cut(s)	259, 811, 1457, 1643
BsgI	GTGCAG	2 cut(s)	600, 614
BshFI	GGCC	7 cut(s)	368, 954, 960, 1034, 1421, 1443, 1529
BsiSI	CCGG	3 cut(s)	928, 1369, 1445
BslFI	GGGAC	3 cut(s)	14, 242, 1305
BslI	CCNNNNNNNGG	3 cut(s)	551, 563, 1416
BsmFI	GGGAC	3 cut(s)	14, 242, 1305
BsmI	GAATGC	1 cut(s)	1621
BsnI	GGCC	7 cut(s)	368, 954, 960, 1034, 1421, 1443, 1529
Bsp119I	TTCGAA	1 cut(s)	1624
Bsp120I	GGGCCC	1 cut(s)	958
Bsp1286I	GDGCHC	4 cut(s)	87, 207, 218, 962
Bsp143I	GATC	6 cut(s)	163, 346, 404, 606, 883, 1123
Bsp19I	CCATGG	1 cut(s)	1681
BspACI	CCGC	2 cut(s)	1388, 1697
BspANI	GGCC	7 cut(s)	368, 954, 960, 1034, 1421, 1443, 1529
BspCNI	CTCAG	6 cut(s)	77, 298, 342, 826, 1336, 1480
BspHI	TCATGA	1 cut(s)	1353
BspLI	GGNNCC	4 cut(s)	204, 960, 1201, 1293
BspMI	ACCTGC	1 cut(s)	575
BspPI	GGATC	4 cut(s)	158, 412, 601, 878
BspT104I	TTCGAA	1 cut(s)	1624
BsrBI	CCGCTC	1 cut(s)	1697
BsrDI	GCAATG	1 cut(s)	273
BsrFI	RCCGGY	2 cut(s)	927, 1368
BsrI	ACTGG	7 cut(s)	32, 183, 254, 1011, 1109, 1205, 1641
BssAI	RCCGGY	2 cut(s)	927, 1368
BssECI	CCNNGG	3 cut(s)	369, 955, 1681
BssMI	GATC	6 cut(s)	163, 346, 404, 606, 883, 1123
BssNI	GRCGYC	1 cut(s)	799
BssT1I	CCWWGG	2 cut(s)	369, 1681
Bst2UI	CCWGG	5 cut(s)	57, 901, 956, 1296, 1531
Bst4CI	ACNGT	6 cut(s)	28, 481, 489, 599, 949, 1150
Bst6I	CTCTTC	2 cut(s)	901, 960
BstACI	GRCGYC	1 cut(s)	799
BstAPI	GCANNNNNTGC	2 cut(s)	1238, 1640
BstBI	TTCGAA	1 cut(s)	1624
BstC8I	GCNNGC	1 cut(s)	1513
BstDEI	CTNAG	6 cut(s)	64, 306, 329, 834, 1344, 1488
BstDSI	CCRYGG	1 cut(s)	1681
BstEII	GGTNACC	1 cut(s)	1103
BstF5I	GGATG	4 cut(s)	622, 981, 1168, 1453
BstHHI	GCGC	3 cut(s)	1243, 1279, 1397
BstKTI	GATC	6 cut(s)	166, 349, 407, 609, 886, 1126
BstMBI	GATC	6 cut(s)	163, 346, 404, 606, 883, 1123
BstMWI	GCNNNNNNNGC	8 cut(s)	503, 659, 1031, 1238, 1637, 1640, 1646, 1655
BstNI	CCWGG	5 cut(s)	57, 901, 956, 1296, 1531
BstPI	GGTNACC	1 cut(s)	1103
BstSCI	CCNGG	6 cut(s)	55, 899, 954, 1294, 1443, 1529
BstSLI	GKGCMC	2 cut(s)	87, 962
BstV1I	GCAGC	6 cut(s)	593, 649, 667, 670, 1627, 1643
BstX2I	RGATCY	2 cut(s)	163, 404
BstXI	CCANNNNNNTGG	1 cut(s)	1682
BstYI	RGATCY	2 cut(s)	163, 404
Bsu36I	CCTNAGG	1 cut(s)	834
BsuRI	GGCC	7 cut(s)	368, 954, 960, 1034, 1421, 1443, 1529
BtgI	CCRYGG	1 cut(s)	1681
BtsCI	GGATG	4 cut(s)	622, 981, 1168, 1453
BtsI	GCAGTG	1 cut(s)	1397
BtsIMutI	CAGTG	6 cut(s)	20, 477, 1018, 1341, 1397, 1634
BveI	ACCTGC	1 cut(s)	575
Cac8I	GCNNGC	1 cut(s)	1513
CaiI	CAGNNNCTG	3 cut(s)	198, 1115, 1319
CciI	TCATGA	1 cut(s)	1353
CfoI	GCGC	3 cut(s)	1243, 1279, 1397
Cfr10I	RCCGGY	2 cut(s)	927, 1368
Cfr13I	GGNCC	9 cut(s)	554, 838, 958, 959, 1033, 1223, 1292, 1419, 1442
CseI	GACGC	1 cut(s)	1613
CsiI	ACCWGGT	1 cut(s)	1294
Csp6I	GTAC	1 cut(s)	1668
CspCI	CAANNNNNGTGG	2 cut(s)	463, 498
CviAII	CATG	9 cut(s)	650, 707, 790, 1217, 1244, 1354, 1433, 1676, 1682
CviQI	GTAC	1 cut(s)	1668
DdeI	CTNAG	6 cut(s)	64, 306, 329, 834, 1344, 1488
DpnI	GATC	6 cut(s)	165, 348, 406, 608, 885, 1125
DpnII	GATC	6 cut(s)	163, 346, 404, 606, 883, 1123
EaeI	YGGCCR	2 cut(s)	952, 1527
Eam1104I	CTCTTC	2 cut(s)	901, 960
EarI	CTCTTC	2 cut(s)	901, 960
Eco130I	CCWWGG	2 cut(s)	369, 1681
Eco24I	GRGCYC	3 cut(s)	207, 218, 962
Eco32I	GATATC	1 cut(s)	787
Eco47I	GGWCC	4 cut(s)	554, 838, 1223, 1292
Eco57I	CTGAAG	1 cut(s)	984
Eco81I	CCTNAGG	1 cut(s)	834
Eco91I	GGTNACC	1 cut(s)	1103
EcoO109I	RGGNCCY	4 cut(s)	838, 958, 959, 1419
EcoO65I	GGTNACC	1 cut(s)	1103
EcoRII	CCWGG	5 cut(s)	55, 899, 954, 1294, 1529
EcoRV	GATATC	1 cut(s)	787
EcoT14I	CCWWGG	2 cut(s)	369, 1681
EcoT22I	ATGCAT	1 cut(s)	1434
EcoT38I	GRGCYC	3 cut(s)	207, 218, 962
ErhI	CCWWGG	2 cut(s)	369, 1681
FaeI	CATG	9 cut(s)	653, 710, 793, 1220, 1247, 1357, 1436, 1679, 1685
FaqI	GGGAC	3 cut(s)	14, 242, 1305
FatI	CATG	9 cut(s)	649, 706, 789, 1216, 1243, 1353, 1432, 1675, 1681
FblI	GTMKAC	1 cut(s)	470
Fnu4HI	GCNGC	6 cut(s)	582, 663, 681, 684, 1632, 1641
FokI	GGATG	4 cut(s)	629, 988, 1175, 1460
FriOI	GRGCYC	3 cut(s)	207, 218, 962
Fsp4HI	GCNGC	6 cut(s)	582, 663, 681, 684, 1632, 1641
FspBI	CTAG	5 cut(s)	231, 408, 677, 870, 1049
FspI	TGCGCA	2 cut(s)	1242, 1396
GlaI	GCGC	3 cut(s)	1242, 1278, 1396
GluI	GCNGC	6 cut(s)	582, 663, 681, 684, 1632, 1641
GsaI	CCCAGC	4 cut(s)	263, 815, 1461, 1647
GsuI	CTGGAG	1 cut(s)	922
HaeIII	GGCC	7 cut(s)	368, 954, 960, 1034, 1421, 1443, 1529
HapII	CCGG	3 cut(s)	928, 1369, 1445
HgaI	GACGC	1 cut(s)	1613
HhaI	GCGC	3 cut(s)	1243, 1279, 1397
Hin1I	GRCGYC	1 cut(s)	799
Hin1II	CATG	9 cut(s)	653, 710, 793, 1220, 1247, 1357, 1436, 1679, 1685
Hin6I	GCGC	3 cut(s)	1241, 1277, 1395
HinP1I	GCGC	3 cut(s)	1241, 1277, 1395
HincII	GTYRAC	3 cut(s)	134, 542, 1726
HindII	GTYRAC	3 cut(s)	134, 542, 1726
HindIII	AAGCTT	1 cut(s)	495
HinfI	GANTC	6 cut(s)	6, 60, 195, 890, 1189, 1598
HpaII	CCGG	3 cut(s)	928, 1369, 1445
HphI	GGTGA	6 cut(s)	12, 243, 889, 991, 1115, 1664
Hpy166II	GTNNAC	7 cut(s)	20, 134, 298, 471, 542, 1582, 1726
Hpy188I	TCNGA	5 cut(s)	307, 724, 1088, 1136, 1603
Hpy188III	TCNNGA	5 cut(s)	335, 512, 887, 1193, 1354
Hpy8I	GTNNAC	7 cut(s)	20, 134, 298, 471, 542, 1582, 1726
Hpy99I	CGWCG	1 cut(s)	1124
HpyAV	CCTTC	5 cut(s)	455, 514, 545, 758, 1153
HpyCH4III	ACNGT	6 cut(s)	28, 481, 489, 599, 949, 1150
HpyCH4IV	ACGT	1 cut(s)	799
HpyF10VI	GCNNNNNNNGC	8 cut(s)	503, 659, 1031, 1238, 1637, 1640, 1646, 1655
HpyF3I	CTNAG	6 cut(s)	64, 306, 329, 834, 1344, 1488
HpySE526I	ACGT	1 cut(s)	799
Hsp92I	GRCGYC	1 cut(s)	799
Hsp92II	CATG	9 cut(s)	653, 710, 793, 1220, 1247, 1357, 1436, 1679, 1685
HspAI	GCGC	3 cut(s)	1241, 1277, 1395
Kzo9I	GATC	6 cut(s)	163, 346, 404, 606, 883, 1123
LmnI	GCTCC	2 cut(s)	202, 1694
Lsp1109I	GCAGC	6 cut(s)	593, 649, 667, 670, 1627, 1643
LweI	GCATC	2 cut(s)	1153, 1645
MabI	ACCWGGT	1 cut(s)	1294
MaeI	CTAG	5 cut(s)	231, 408, 677, 870, 1049
MaeII	ACGT	1 cut(s)	799
MaeIII	GTNAC	6 cut(s)	526, 593, 1103, 1111, 1309, 1703
MalI	GATC	6 cut(s)	165, 348, 406, 608, 885, 1125
MbiI	CCGCTC	1 cut(s)	1697
MboI	GATC	6 cut(s)	163, 346, 404, 606, 883, 1123
MboII	GAAGA	5 cut(s)	93, 665, 863, 918, 977
MflI	RGATCY	2 cut(s)	163, 404
MhlI	GDGCHC	4 cut(s)	87, 207, 218, 962
MlsI	TGGCCA	2 cut(s)	954, 1529
MluCI	AATT	9 cut(s)	280, 567, 863, 1249, 1349, 1398, 1522, 1610, 1717
MluNI	TGGCCA	2 cut(s)	954, 1529
MlyI	GAGTC	2 cut(s)	1183, 1592
MmeI	TCCRAC	3 cut(s)	219, 463, 1114
Mox20I	TGGCCA	2 cut(s)	954, 1529
Mph1103I	ATGCAT	1 cut(s)	1434
MroXI	GAANNNNTTC	1 cut(s)	1586
MscI	TGGCCA	2 cut(s)	954, 1529
MslI	CAYNNNNRTG	3 cut(s)	1390, 1437, 1680
Msp20I	TGGCCA	2 cut(s)	954, 1529
MspA1I	CMGCKG	2 cut(s)	263, 662
MspI	CCGG	3 cut(s)	928, 1369, 1445
MspR9I	CCNGG	6 cut(s)	57, 901, 956, 1296, 1445, 1531
Mva1269I	GAATGC	1 cut(s)	1621
MvaI	CCWGG	5 cut(s)	57, 901, 956, 1296, 1531
MwoI	GCNNNNNNNGC	8 cut(s)	503, 659, 1031, 1238, 1637, 1640, 1646, 1655
NciI	CCSGG	1 cut(s)	1445
NcoI	CCATGG	1 cut(s)	1681
NdeII	GATC	6 cut(s)	163, 346, 404, 606, 883, 1123
NlaIII	CATG	9 cut(s)	653, 710, 793, 1220, 1247, 1357, 1436, 1679, 1685
NlaIV	GGNNCC	4 cut(s)	204, 960, 1201, 1293
NmuCI	GTSAC	4 cut(s)	526, 1103, 1309, 1703
NsbI	TGCGCA	2 cut(s)	1242, 1396
NsiI	ATGCAT	1 cut(s)	1434
NspV	TTCGAA	1 cut(s)	1624
OliI	CACNNNNGTG	1 cut(s)	1390
PagI	TCATGA	1 cut(s)	1353
PaqCI	CACCTGC	1 cut(s)	575
PctI	GAATGC	1 cut(s)	1621
PdmI	GAANNNNTTC	1 cut(s)	1586
PfeI	GAWTC	3 cut(s)	60, 195, 890
PflFI	GACNNNGTC	1 cut(s)	489
PflMI	CCANNNNNTGG	1 cut(s)	563
PfoI	TCCNGGA	1 cut(s)	55
PkrI	GCNGC	6 cut(s)	583, 664, 682, 685, 1633, 1642
PleI	GAGTC	2 cut(s)	1183, 1592
PpsI	GAGTC	2 cut(s)	1183, 1592
PpuMI	RGGWCCY	1 cut(s)	838
PshBI	ATTAAT	1 cut(s)	1181
Psp5II	RGGWCCY	1 cut(s)	838
Psp6I	CCWGG	5 cut(s)	55, 899, 954, 1294, 1529
PspEI	GGTNACC	1 cut(s)	1103
PspFI	CCCAGC	4 cut(s)	259, 811, 1457, 1643
PspGI	CCWGG	5 cut(s)	55, 899, 954, 1294, 1529
PspN4I	GGNNCC	4 cut(s)	204, 960, 1201, 1293
PspOMI	GGGCCC	1 cut(s)	958
PspPI	GGNCC	9 cut(s)	554, 838, 958, 959, 1033, 1223, 1292, 1419, 1442
PspPPI	RGGWCCY	1 cut(s)	838
PstNI	CAGNNNCTG	3 cut(s)	198, 1115, 1319
PsuI	RGATCY	2 cut(s)	163, 404
PsyI	GACNNNGTC	1 cut(s)	489
PvuII	CAGCTG	2 cut(s)	263, 662
RsaI	GTAC	1 cut(s)	1669
RsaNI	GTAC	1 cut(s)	1668
RseI	CAYNNNNRTG	3 cut(s)	1390, 1437, 1680
SatI	GCNGC	6 cut(s)	582, 663, 681, 684, 1632, 1641
Sau3AI	GATC	6 cut(s)	163, 346, 404, 606, 883, 1123
Sau96I	GGNCC	9 cut(s)	554, 838, 958, 959, 1033, 1223, 1292, 1419, 1442
SchI	GAGTC	2 cut(s)	1183, 1592
ScrFI	CCNGG	6 cut(s)	57, 901, 956, 1296, 1445, 1531
SduI	GDGCHC	4 cut(s)	87, 207, 218, 962
SexAI	ACCWGGT	1 cut(s)	1294
SfaNI	GCATC	2 cut(s)	1153, 1645
SfuI	TTCGAA	1 cut(s)	1624
SinI	GGWCC	4 cut(s)	554, 838, 1223, 1292
SmiMI	CAYNNNNRTG	3 cut(s)	1390, 1437, 1680
SmlI	CTYRAG	1 cut(s)	1193
SmoI	CTYRAG	1 cut(s)	1193
Sse9I	AATT	9 cut(s)	280, 567, 863, 1249, 1349, 1398, 1522, 1610, 1717
SsiI	CCGC	2 cut(s)	1388, 1697
SspI	AATATT	1 cut(s)	1237
SspMI	CTAG	5 cut(s)	231, 408, 677, 870, 1049
StyD4I	CCNGG	6 cut(s)	55, 899, 954, 1294, 1443, 1529
StyI	CCWWGG	2 cut(s)	369, 1681
TaaI	ACNGT	6 cut(s)	28, 481, 489, 599, 949, 1150
TaiI	ACGT	1 cut(s)	802
TaqI	TCGA	4 cut(s)	69, 104, 697, 1624
TasI	AATT	9 cut(s)	280, 567, 863, 1249, 1349, 1398, 1522, 1610, 1717
TatI	WGTACW	1 cut(s)	1667
TfiI	GAWTC	3 cut(s)	60, 195, 890
TscAI	CASTG	6 cut(s)	20, 484, 1018, 1348, 1397, 1641
TseFI	GTSAC	4 cut(s)	526, 1103, 1309, 1703
TseI	GCWGC	6 cut(s)	581, 662, 680, 683, 1631, 1640
Tsp45I	GTSAC	4 cut(s)	526, 1103, 1309, 1703
TspDTI	ATGAA	8 cut(s)	408, 638, 723, 752, 1172, 1205, 1342, 1370
TspRI	CASTG	6 cut(s)	20, 484, 1018, 1348, 1397, 1641
Tth111I	GACNNNGTC	1 cut(s)	489
Van91I	CCANNNNNTGG	1 cut(s)	563
VpaK11BI	GGWCC	4 cut(s)	554, 838, 1223, 1292
VspI	ATTAAT	1 cut(s)	1181
XapI	RAATTY	5 cut(s)	863, 1249, 1349, 1522, 1717
XcmI	CCANNNNNNNNNTGG	3 cut(s)	235, 1018, 1682
XmiI	GTMKAC	1 cut(s)	470
XmnI	GAANNNNTTC	1 cut(s)	1586
XspI	CTAG	5 cut(s)	231, 408, 677, 870, 1049
ZraI	GACGTC	1 cut(s)	800
Zsp2I	ATGCAT	1 cut(s)	1434

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.