MD03G1042100.v1.1

PPR repeat

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0013877
Physical Location & Seq
Forward (+)
3320529 .. 3322208
1680 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1042100.v1.1.491

Sequence Viewer

Length: 1680 bp
ATGGCTGCTCTCGCTCCTCCGTCTCTTCTCAATCATAACAACCTGCCATCCTTCTCTCCGAAAATCGCAGTGAGAATGCCAACCACTCTGGTTTTGCTTCAAATGTGCTGCAACTTCCAAGAAGTGAGACAGGTGCACGCTCAGTTGGTTGTATCCGGCCTCCTTGACCGCCCTCTAAACGCCGGTAGGCTTCTCCAGTCCTACGTGATAATTTCGCAAATTCACCACGCCTTGTCGATTTTCAATAGAATATCTTATCCTGATGTATTTGCCTACAACAACATGATCAGAGGATTAATTTTGGTTAAGTCCCCATATCACTCGTTGTTATTGTATAACAAGCTTTTACTAGATAGATTGAAACCAGACAATTACACTTACACCTTTGTTCTTAAAGCATGCTCCCAACTGAATGCACTCTTTGAAGGCAGACAAGTGCATTGCCGGATAGTTAAGGCCGGGATAGCTATGGATACTCACGTTCACAGCTCACTAATACACATGTACTCGAGTTCAACGGACAGTTTGGATTGCGCAGAACAAGTACTCGCAGGGTTCCCCGAGGAAAACACGCTTGCAAAGAACTCCTTAATTTCGGGGTACCTTAGATGGGGTCAAGTAGAGAAGGCTAGAGCAATGTTTGATAACATGGTGGCAAAAGATGATGCATCTTGGAGCGCAATGATAGGAGGGTACACAAAGAACGGTATGTATGCAGATGCATTAGGGATTTTCCGAAAAATGACGATGATTTTGAAGGTTTCTGCAAACGAATCGACCCTAGTGAGCTCATTGTCTGCCTGTGCTCATTTGGGAGCTCTAGATCAAGGGAAATGGATACATACATATGCCGATAAAATCGGGGCTAAGATTAGTGTGACTCTTGGCACTGCTCTCATAGACATGTATGCCAAGTGTGGCAGCATTGAACACGGTTACGAAATCTTTCGGAAGATGTCACAAAAAGATACAGTTACTTGGGGAGCTATGATTTCTGGTTTTGCCATACATGGGGATGCCGAGAAATGTTTCGAATTGTTTGATGAAATGGTTGCGAACGGAATGTATCCGAACGAAGTCATTTTTGTGGCTATTTTATCTGTCTGTTCCCATGCCGGGCGTGTCGAAATGGGATACGAGTACTTTCACAAAATGGTGCATGACTATGGGATAAGACCATCCATTGAACATTATGGATGCATGGTAGACCTTCTCGGCCGTGCCGGGCGGCTAGAAGAGGCGGAAGAGCTCATCATGTCAATGCCGGAAAACCCCAATTCGGTTATTTGGGGTGCGTTGCTTGGCGCTTGTCGGACGCATGGTGACCTGAGGAGAGGGAACCTTGCATTCAAGCACTTGACTGAACTGGAACCAATGTCTGGTGACAGATACAAACTAGCAGGGATTATGTTTGCCAATACTGGAGAAAAGGAAAGTGCAACTGAAATTCAGAAGCTCATCGGAGAAAACGAATTGGAGACTAGTCGTGGGATGAGCTTCATCGAAATAGATGGCGTCGTCCATGAGTTTGTAGCGGGGACTATTCATCATAGCAGGCACAAAGAAATCTACAACATGTGGGACAAGGTTCATAGATTGTTGGTCATTGATGAAACCGAAATTGGAACTCCATTCTTTACGTTGGAAAATCGCAATAAAGATGAGGATGCCACATCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PPR PPR_2

Protein Analysis

560

Amino Acids

62.47

Weight (kDa)

6.18

Isoelectric Point (pI)

39.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 87 - 135 5.7e-07 PPR repeat family
PPR PF01535 224 - 248 8.1e-06 PPR repeat
PPR_long PF17177 270 - 387 1.9e-08 Pentacotripeptide-repeat region of PRORP
PPR_2 PF13041 322 - 360 3.8e-08 PPR repeat family
PPR PF01535 325 - 354 3.8e-08 PPR repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013877)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G28280 AT4G28280 AT4G28280
malus_domestica MD03G1042100.v1.1 MD11G1044400.v1.1 MD11G1045000.v1.1
prunus_persica Prupe.6G034000_v2.0.a1
pyrus_communis pycom03g03260
rosa_chinensis RchiOBHm_Chr5g0074761
rosa_laevigata RLG00000036502
rosa_multiflora Rmu_sc0000909.1_g000018
rosa_roxburghii Rroxscaffold_1G00006820
rosa_rugosa Rorug05G0433700
rosa_samantha Rh5AG491400 Rh5BG512800 Rh5CG536500
rosa_wichuraiana Rw5G045510

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 535
Acc36I ACCTGC 1 cut(s) 51
Acc65I GGTACC 1 cut(s) 600
AccB1I GGYRCC 1 cut(s) 600
AccB7I CCANNNNNTGG 1 cut(s) 1379
AccI GTMKAC 1 cut(s) 1206
AciI CCGC 4 cut(s) 169, 1228, 1241, 1535
AcoI YGGCCR 1 cut(s) 1216
AcsI RAATTY 2 cut(s) 219, 1446
AcyI GRCGYC 1 cut(s) 1515
AfaI GTAC 5 cut(s) 506, 546, 602, 695, 1142
AfiI CCNNNNNNNGG 5 cut(s) 610, 1011, 1116, 1279, 1379
AflIII ACRYGT 3 cut(s) 501, 903, 1575
AgsI TTSAA 9 cut(s) 101, 244, 361, 425, 516, 757, 929, 1187, 1351
AhlI ACTAGT 1 cut(s) 1481
AjuI GAANNNNNNNTTGG 2 cut(s) 1605, 1637
AluBI AGCT 9 cut(s) 343, 467, 489, 789, 818, 986, 1249, 1456, 1497
AluI AGCT 9 cut(s) 343, 467, 489, 789, 818, 986, 1249, 1456, 1497
Alw21I GWGCWC 5 cut(s) 138, 791, 808, 820, 1251
Alw26I GTCTC 3 cut(s) 27, 121, 1472
Alw44I GTGCAC 1 cut(s) 134
Ama87I CYCGRG 2 cut(s) 508, 560
AoxI GGCC 3 cut(s) 157, 456, 1216
ApaLI GTGCAC 1 cut(s) 134
ApeKI GCWGC 3 cut(s) 5, 108, 921
ApoI RAATTY 2 cut(s) 219, 1446
ArsI GACNNNNNNTTYG 2 cut(s) 736, 768
AseI ATTAAT 1 cut(s) 296
Asp700I GAANNNNTTC 2 cut(s) 945, 1028
Asp718I GGTACC 1 cut(s) 600
AspLEI GCGC 3 cut(s) 536, 680, 1307
AsuC2I CCSGG 3 cut(s) 460, 1117, 1225
AsuHPI GGTGA 3 cut(s) 215, 1334, 1394
AsuII TTCGAA 1 cut(s) 1032
AvaI CYCGRG 2 cut(s) 508, 560
AxyI CCTNAGG 1 cut(s) 1328
BaeGI GKGCMC 1 cut(s) 138
BaeI ACNNNNGTAYC 2 cut(s) 465, 498
BanI GGYRCC 1 cut(s) 600
BanII GRGCYC 3 cut(s) 791, 820, 1251
Bbv12I GWGCWC 5 cut(s) 138, 791, 808, 820, 1251
BbvI GCAGC 2 cut(s) 95, 933
BccI CCATC 4 cut(s) 55, 603, 1186, 1505
BceAI ACGGC 1 cut(s) 1203
BcgI CGANNNNNNTGC 2 cut(s) 756, 790
BciVI GTATCC 5 cut(s) 163, 466, 831, 1077, 1127
BclI TGATCA 1 cut(s) 285
BcnI CCSGG 3 cut(s) 460, 1117, 1225
BcoDI GTCTC 3 cut(s) 27, 121, 1472
BcuI ACTAGT 1 cut(s) 1481
BfaI CTAG 7 cut(s) 350, 630, 782, 821, 1232, 1397, 1482
BfoI RGCGCY 1 cut(s) 1308
BfuAI ACCTGC 1 cut(s) 51
BfuI GTATCC 5 cut(s) 163, 466, 831, 1077, 1127
BisI GCNGC 4 cut(s) 6, 109, 922, 1229
BlsI GCNGC 4 cut(s) 7, 110, 923, 1230
BmcAI AGTACT 2 cut(s) 546, 1142
Bme1390I CCNGG 3 cut(s) 460, 1117, 1225
BmeT110I CYCGRG 2 cut(s) 508, 560
BmiI GGNNCC 4 cut(s) 557, 602, 1340, 1371
BmrFI CCNGG 3 cut(s) 460, 1117, 1225
BmsI GCATC 6 cut(s) 655, 677, 709, 1006, 1187, 1657
BpmI CTGGAG 2 cut(s) 179, 1443
Bpu14I TTCGAA 1 cut(s) 1032
BpuMI CCSGG 3 cut(s) 460, 1117, 1225
BsaAI YACGTR 1 cut(s) 205
BsaHI GRCGYC 1 cut(s) 1515
BsaJI CCNNGG 1 cut(s) 561
Bsc4I CCNNNNNNNGG 5 cut(s) 610, 1011, 1116, 1279, 1379
Bse118I RCCGGY 1 cut(s) 182
Bse1I ACTGG 3 cut(s) 196, 1371, 1426
Bse21I CCTNAGG 1 cut(s) 1328
Bse3DI GCAATG 3 cut(s) 439, 642, 687
BseDI CCNNGG 1 cut(s) 561
BseGI GGATG 6 cut(s) 47, 1021, 1178, 1202, 1497, 1672
BseLI CCNNNNNNNGG 5 cut(s) 610, 1011, 1116, 1279, 1379
BseMI GCAATG 3 cut(s) 439, 642, 687
BseMII CTCAG 2 cut(s) 155, 1319
BseNI ACTGG 3 cut(s) 196, 1371, 1426
BseRI GAGGAG 2 cut(s) 6, 1345
BseSI GKGCMC 1 cut(s) 138
BseX3I CGGCCG 1 cut(s) 1216
BseXI GCAGC 2 cut(s) 95, 933
Bsh1285I CGRYCG 1 cut(s) 1219
BshFI GGCC 3 cut(s) 159, 458, 1218
BshNI GGYRCC 1 cut(s) 600
BsiEI CGRYCG 1 cut(s) 1219
BsiHKAI GWGCWC 5 cut(s) 138, 791, 808, 820, 1251
BsiHKCI CYCGRG 2 cut(s) 508, 560
BsiSI CCGG 7 cut(s) 156, 183, 445, 459, 1116, 1224, 1265
BslFI GGGAC 3 cut(s) 295, 1552, 1595
BslI CCNNNNNNNGG 5 cut(s) 610, 1011, 1116, 1279, 1379
BsmAI GTCTC 3 cut(s) 27, 121, 1472
BsmBI CGTCTC 1 cut(s) 27
BsmFI GGGAC 3 cut(s) 295, 1552, 1595
BsmI GAATGC 3 cut(s) 81, 418, 1346
BsnI GGCC 3 cut(s) 159, 458, 1218
BsoBI CYCGRG 2 cut(s) 508, 560
Bsp119I TTCGAA 1 cut(s) 1032
Bsp1286I GDGCHC 5 cut(s) 138, 791, 808, 820, 1251
Bsp143I GATC 2 cut(s) 285, 823
BspACI CCGC 4 cut(s) 169, 1228, 1241, 1535
BspANI GGCC 3 cut(s) 159, 458, 1218
BspCNI CTCAG 2 cut(s) 154, 1320
BspHI TCATGA 1 cut(s) 1676
BspLI GGNNCC 4 cut(s) 557, 602, 1340, 1371
BspMI ACCTGC 1 cut(s) 51
BspQI GCTCTTC 1 cut(s) 1239
BspT104I TTCGAA 1 cut(s) 1032
BspT107I GGYRCC 1 cut(s) 600
BsrDI GCAATG 3 cut(s) 439, 642, 687
BsrFI RCCGGY 1 cut(s) 182
BsrI ACTGG 3 cut(s) 196, 1371, 1426
BssAI RCCGGY 1 cut(s) 182
BssECI CCNNGG 1 cut(s) 561
BssMI GATC 2 cut(s) 285, 823
BssNI GRCGYC 1 cut(s) 1515
Bst4CI ACNGT 4 cut(s) 524, 707, 935, 973
Bst6I CTCTTC 3 cut(s) 30, 1230, 1239
BstACI GRCGYC 1 cut(s) 1515
BstBAI YACGTR 1 cut(s) 205
BstBI TTCGAA 1 cut(s) 1032
BstC8I GCNNGC 4 cut(s) 138, 400, 576, 1556
BstDEI CTNAG 4 cut(s) 141, 605, 867, 1328
BstEII GGTNACC 1 cut(s) 1322
BstF5I GGATG 6 cut(s) 47, 1021, 1178, 1202, 1497, 1672
BstH2I RGCGCY 1 cut(s) 1308
BstHHI GCGC 3 cut(s) 536, 680, 1307
BstKTI GATC 2 cut(s) 288, 826
BstMAI GTCTC 3 cut(s) 27, 121, 1472
BstMBI GATC 2 cut(s) 285, 823
BstMCI CGRYCG 1 cut(s) 1219
BstMWI GCNNNNNNNGC 2 cut(s) 11, 464
BstNSI RCATGY 4 cut(s) 402, 505, 907, 1579
BstPI GGTNACC 1 cut(s) 1322
BstSCI CCNGG 3 cut(s) 458, 1115, 1223
BstSLI GKGCMC 1 cut(s) 138
BstV1I GCAGC 2 cut(s) 95, 933
BstZI CGGCCG 1 cut(s) 1216
Bsu36I CCTNAGG 1 cut(s) 1328
BsuI GTATCC 5 cut(s) 163, 466, 831, 1077, 1127
BsuRI GGCC 3 cut(s) 159, 458, 1218
BtsCI GGATG 6 cut(s) 47, 1021, 1178, 1202, 1497, 1672
BtsI GCAGTG 2 cut(s) 75, 888
BtsIMutI CAGTG 2 cut(s) 75, 888
BveI ACCTGC 1 cut(s) 51
Cac8I GCNNGC 4 cut(s) 138, 400, 576, 1556
CciI TCATGA 1 cut(s) 1676
CfoI GCGC 3 cut(s) 536, 680, 1307
Cfr10I RCCGGY 1 cut(s) 182
CseI GACGC 2 cut(s) 1324, 1504
Csp6I GTAC 5 cut(s) 505, 545, 601, 694, 1141
CviQI GTAC 5 cut(s) 505, 545, 601, 694, 1141
DdeI CTNAG 4 cut(s) 141, 605, 867, 1328
DpnI GATC 2 cut(s) 287, 825
DpnII GATC 2 cut(s) 285, 823
EaeI YGGCCR 1 cut(s) 1216
EagI CGGCCG 1 cut(s) 1216
Eam1104I CTCTTC 3 cut(s) 30, 1230, 1239
EarI CTCTTC 3 cut(s) 30, 1230, 1239
EciI GGCGGA 1 cut(s) 1256
Ecl136II GAGCTC 3 cut(s) 789, 818, 1249
EclXI CGGCCG 1 cut(s) 1216
Eco24I GRGCYC 3 cut(s) 791, 820, 1251
Eco52I CGGCCG 1 cut(s) 1216
Eco53kI GAGCTC 3 cut(s) 789, 818, 1249
Eco81I CCTNAGG 1 cut(s) 1328
Eco88I CYCGRG 2 cut(s) 508, 560
Eco91I GGTNACC 1 cut(s) 1322
EcoICRI GAGCTC 3 cut(s) 789, 818, 1249
EcoO65I GGTNACC 1 cut(s) 1322
EcoT22I ATGCAT 3 cut(s) 670, 724, 1202
EcoT38I GRGCYC 3 cut(s) 791, 820, 1251
Esp3I CGTCTC 1 cut(s) 27
FalI AAGNNNNNCTT 2 cut(s) 572, 604
FaqI GGGAC 3 cut(s) 295, 1552, 1595
FauI CCCGC 1 cut(s) 1528
FauNDI CATATG 1 cut(s) 847
FbaI TGATCA 1 cut(s) 285
FblI GTMKAC 1 cut(s) 1206
Fnu4HI GCNGC 4 cut(s) 6, 109, 922, 1229
FokI GGATG 5 cut(s) 34, 1028, 1165, 1209, 1504
FriOI GRGCYC 3 cut(s) 791, 820, 1251
Fsp4HI GCNGC 4 cut(s) 6, 109, 922, 1229
FspBI CTAG 7 cut(s) 350, 630, 782, 821, 1232, 1397, 1482
FspI TGCGCA 1 cut(s) 535
GlaI GCGC 3 cut(s) 535, 679, 1306
GluI GCNGC 4 cut(s) 6, 109, 922, 1229
GsuI CTGGAG 2 cut(s) 179, 1443
HaeII RGCGCY 1 cut(s) 1308
HaeIII GGCC 3 cut(s) 159, 458, 1218
HapII CCGG 7 cut(s) 156, 183, 445, 459, 1116, 1224, 1265
HgaI GACGC 2 cut(s) 1324, 1504
HhaI GCGC 3 cut(s) 536, 680, 1307
Hin1I GRCGYC 1 cut(s) 1515
Hin6I GCGC 3 cut(s) 534, 678, 1305
HinP1I GCGC 3 cut(s) 534, 678, 1305
HindIII AAGCTT 1 cut(s) 341
HinfI GANTC 2 cut(s) 773, 880
HpaII CCGG 7 cut(s) 156, 183, 445, 459, 1116, 1224, 1265
HphI GGTGA 3 cut(s) 215, 1334, 1394
Hpy166II GTNNAC 4 cut(s) 136, 484, 696, 1207
Hpy188I TCNGA 8 cut(s) 60, 290, 737, 951, 1071, 1314, 1452, 1463
Hpy188III TCNNGA 3 cut(s) 260, 821, 1677
Hpy8I GTNNAC 4 cut(s) 136, 484, 696, 1207
Hpy99I CGWCG 1 cut(s) 1520
HpyAV CCTTC 5 cut(s) 61, 419, 619, 751, 1220
HpyCH4III ACNGT 4 cut(s) 524, 707, 935, 973
HpyCH4IV ACGT 3 cut(s) 204, 480, 1640
HpyF10VI GCNNNNNNNGC 2 cut(s) 11, 464
HpyF3I CTNAG 4 cut(s) 141, 605, 867, 1328
HpySE526I ACGT 3 cut(s) 204, 480, 1640
Hsp92I GRCGYC 1 cut(s) 1515
HspAI GCGC 3 cut(s) 534, 678, 1305
KpnI GGTACC 1 cut(s) 604
Ksp22I TGATCA 1 cut(s) 285
Kzo9I GATC 2 cut(s) 285, 823
LguI GCTCTTC 1 cut(s) 1239
LmnI GCTCC 5 cut(s) 19, 407, 675, 815, 983
Lsp1109I GCAGC 2 cut(s) 95, 933
LweI GCATC 6 cut(s) 655, 677, 709, 1006, 1187, 1657
MaeI CTAG 7 cut(s) 350, 630, 782, 821, 1232, 1397, 1482
MaeII ACGT 3 cut(s) 204, 480, 1640
MaeIII GTNAC 6 cut(s) 877, 935, 957, 973, 1322, 1382
MalI GATC 2 cut(s) 287, 825
MboI GATC 2 cut(s) 285, 823
MboII GAAGA 4 cut(s) 17, 964, 1247, 1256
MhlI GDGCHC 5 cut(s) 138, 791, 808, 820, 1251
MlyI GAGTC 1 cut(s) 874
MmeI TCCRAC 2 cut(s) 1292, 1623
Mph1103I ATGCAT 3 cut(s) 670, 724, 1202
MroXI GAANNNNTTC 2 cut(s) 945, 1028
MseI TTAA 5 cut(s) 296, 306, 393, 453, 590
MslI CAYNNNNRTG 6 cut(s) 846, 902, 1014, 1085, 1164, 1259
MspI CCGG 7 cut(s) 156, 183, 445, 459, 1116, 1224, 1265
MspR9I CCNGG 3 cut(s) 460, 1117, 1225
Mva1269I GAATGC 3 cut(s) 81, 418, 1346
MwoI GCNNNNNNNGC 2 cut(s) 11, 464
NciI CCSGG 3 cut(s) 460, 1117, 1225
NdeI CATATG 1 cut(s) 847
NdeII GATC 2 cut(s) 285, 823
NlaIV GGNNCC 4 cut(s) 557, 602, 1340, 1371
NmeAIII GCCGAG 2 cut(s) 1045, 1194
NmuCI GTSAC 4 cut(s) 877, 957, 1322, 1382
NsbI TGCGCA 1 cut(s) 535
NsiI ATGCAT 3 cut(s) 670, 724, 1202
NspI RCATGY 4 cut(s) 402, 505, 907, 1579
NspV TTCGAA 1 cut(s) 1032
PaeI GCATGC 1 cut(s) 402
PaeR7I CTCGAG 1 cut(s) 508
PagI TCATGA 1 cut(s) 1676
PciI ACATGT 3 cut(s) 501, 903, 1575
PciSI GCTCTTC 1 cut(s) 1239
PcsI WCGNNNNNNNCGW 1 cut(s) 1467
PctI GAATGC 3 cut(s) 81, 418, 1346
PdmI GAANNNNTTC 2 cut(s) 945, 1028
PfeI GAWTC 1 cut(s) 773
PflMI CCANNNNNTGG 1 cut(s) 1379
PkrI GCNGC 4 cut(s) 7, 110, 923, 1230
PleI GAGTC 1 cut(s) 874
PpsI GAGTC 1 cut(s) 874
Ppu21I YACGTR 1 cut(s) 205
PscI ACATGT 3 cut(s) 501, 903, 1575
PshBI ATTAAT 1 cut(s) 296
Psp124BI GAGCTC 3 cut(s) 791, 820, 1251
PspEI GGTNACC 1 cut(s) 1322
PspN4I GGNNCC 4 cut(s) 557, 602, 1340, 1371
PspXI VCTCGAGB 1 cut(s) 508
RsaI GTAC 5 cut(s) 506, 546, 602, 695, 1142
RsaNI GTAC 5 cut(s) 505, 545, 601, 694, 1141
RseI CAYNNNNRTG 6 cut(s) 846, 902, 1014, 1085, 1164, 1259
SacI GAGCTC 3 cut(s) 791, 820, 1251
SapI GCTCTTC 1 cut(s) 1239
SaqAI TTAA 5 cut(s) 296, 306, 393, 453, 590
SatI GCNGC 4 cut(s) 6, 109, 922, 1229
Sau3AI GATC 2 cut(s) 285, 823
ScaI AGTACT 2 cut(s) 546, 1142
SchI GAGTC 1 cut(s) 874
ScrFI CCNGG 3 cut(s) 460, 1117, 1225
SduI GDGCHC 5 cut(s) 138, 791, 808, 820, 1251
SfaNI GCATC 6 cut(s) 655, 677, 709, 1006, 1187, 1657
Sfr274I CTCGAG 1 cut(s) 508
SfuI TTCGAA 1 cut(s) 1032
SlaI CTCGAG 1 cut(s) 508
SmiMI CAYNNNNRTG 6 cut(s) 846, 902, 1014, 1085, 1164, 1259
SmlI CTYRAG 1 cut(s) 508
SmoI CTYRAG 1 cut(s) 508
SpeI ACTAGT 1 cut(s) 1481
SphI GCATGC 1 cut(s) 402
SsiI CCGC 4 cut(s) 169, 1228, 1241, 1535
SspMI CTAG 7 cut(s) 350, 630, 782, 821, 1232, 1397, 1482
SstI GAGCTC 3 cut(s) 791, 820, 1251
StyD4I CCNGG 3 cut(s) 458, 1115, 1223
TaaI ACNGT 4 cut(s) 524, 707, 935, 973
TaiI ACGT 3 cut(s) 207, 483, 1643
TaqI TCGA 6 cut(s) 236, 509, 776, 1032, 1125, 1503
TatI WGTACW 3 cut(s) 504, 544, 1140
TauI GCSGC 1 cut(s) 1231
TfiI GAWTC 1 cut(s) 773
Tru1I TTAA 5 cut(s) 296, 306, 393, 453, 590
Tru9I TTAA 5 cut(s) 296, 306, 393, 453, 590
TscAI CASTG 2 cut(s) 75, 895
TseFI GTSAC 4 cut(s) 877, 957, 1322, 1382
TseI GCWGC 3 cut(s) 5, 108, 921
Tsp45I GTSAC 4 cut(s) 877, 957, 1322, 1382
TspDTI ATGAA 5 cut(s) 1059, 1489, 1535, 1580, 1626
TspGWI ACGGA 3 cut(s) 9, 533, 1074
TspRI CASTG 2 cut(s) 75, 895
Van91I CCANNNNNTGG 1 cut(s) 1379
VneI GTGCAC 1 cut(s) 134
VspI ATTAAT 1 cut(s) 296
XapI RAATTY 2 cut(s) 219, 1446
XbaI TCTAGA 1 cut(s) 820
XceI RCATGY 4 cut(s) 402, 505, 907, 1579
XhoI CTCGAG 1 cut(s) 508
XmiI GTMKAC 1 cut(s) 1206
XmnI GAANNNNTTC 2 cut(s) 945, 1028
XspI CTAG 7 cut(s) 350, 630, 782, 821, 1232, 1397, 1482
ZrmI AGTACT 2 cut(s) 546, 1142
Zsp2I ATGCAT 3 cut(s) 670, 724, 1202
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.