pycom03g03260

PPR repeat

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr3
OG0013877
Physical Location & Seq
Forward (+)
2581219 .. 2582877
1659 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom03g03260.1

Sequence Viewer

Length: 1659 bp
ATGGCTGCTCTCGCTCCTCCGTCTCTTCCCAATCATAACAACCTGCCATCCTTCTCTCCGAAAATCACAGTGAGAATGCCAACCACTCTGATTTTGCTTCAAATGTGCTGCAACCTCCAAGAAGTGAGACAGGTGCATGCTCAGTTGGTTGTATCCGGCCTCCTTGACCGCCCTCTAAATGCCGGTAGGCTTCTCCAGTCCTATGTGATAGTCTCGCAAATTCACCACGCCTTGTCGATTTTCGATAGAATATCTTATCCTGATGTATTTGCCTACAACAACATGATCAGAGGATTAATTTTGGTTAAGTCCCCATATCACTCGTTGTTATTGTATAACAAACTTTTACTAGATAGATTGAAACCAGACAATTACACTTACACCTTTGTTCTTAAAGCATGTTCCCAACTGAATGCACTCTTTGAAGGCAGACAGGTGCATTGCCGGATAGTTAAGGCCGGCGTAGCTATGGATACTCACGTTCACAGCTCACTAATACACATGTACTCGAGTTCAACAGACAGTTTGGATTGCGCAGAACAAGTACTCGCAGGGTTCTCTGAGGAAAACACGCTTGCAAAGAACTCCTTAATTTCTGGGTACCTTAGATGGGGTCAAGTAGAGAAGGCTAGGGCAATGTTTGATAACATGGTGGCAAAAGATGATGCATCTTGGAGCGCAATGGTAGGAGGGTACACAAAGAACGGTATGTATGCAGATGCATTAGGGATTTTCCGAAAAATGACGATGATTTTGAAGGTTTCTGCAAACGAATCGACCCTAGTGAGCTCATTGTCTGCCTGTGCTCATTTGGGAGCTCTAGATCAAGGGAAATGGATACATACATATGCTGATAAAATCGGGGCTAAGATTAGTGTGACTCTTGGCACTGCTCTCATAGACATGTATGCTAAATGTGGCAGCATTGAACACGGTTACGAAATCTTTCGGAAGATGTCACAAAAAGATGCAGTTACTTGGGGAGCTATGATTTCTGGTTTTGCCATACATGGGGATGCCGAGAAATGTTTCGAACTGTTTGATGAAATGGTTGCGAACGGAATGTATCCGAACGAAGTCATTTTTGTGGCTATTTTATCTGCTTGTTCCCATGCCGGGCGTGTCGAAATGGGATACGAGTACTTTTACAAAATGGTGCATGACTATGGGATAAGACCGTCCATTGAACATTATGGATGCATGGTAGACCTTCTCGGCCGTGCCGGGCGGCTAGAAGAGGCGGAAGAGCTCATCATGTCAATGCCGGAAAACCCCAATTCAGTTATCTGGGGTGCGTTGCTTGGCGCTTGTCGGACGCATGGTGACCTGAGGAGAGGGAACCTTGCATTCAAGCACTTGACTGAACTGGAACCAATGTCTGGTGACAGATACAAACTAGCAGGGATTATGTTTGCCAATACTGGAGAAAAGGAAAGTGCAACTGAAATTCAGAAGCTCATCGGAGAAAACGAATTGGAGACTACTCGTGGGATGAGCTTAATCGAAATAGATGGCGTCGTCCATGAGTTTGTAGCGGGGAGTATTCATCATAGCAGGCACAAAGAAATCTACAACATGTGGGACAAGGTTCATAGATTGTTGGTCATTGATGAAACCGCAAAAAATAGCAATACTCCTTACATGCAAACGAGCTTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PPR PPR_2

Protein Analysis

553

Amino Acids

61.55

Weight (kDa)

6.37

Isoelectric Point (pI)

41.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 87 - 135 5.6e-07 PPR repeat family
PPR_long PF17177 270 - 386 8e-07 Pentacotripeptide-repeat region of PRORP
PPR_2 PF13041 322 - 370 9e-09 PPR repeat family
PPR PF01535 325 - 354 3.7e-08 PPR repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013877)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G28280 AT4G28280 AT4G28280
malus_domestica MD03G1042100.v1.1 MD11G1044400.v1.1 MD11G1045000.v1.1
prunus_persica Prupe.6G034000_v2.0.a1
pyrus_communis pycom03g03260
rosa_chinensis RchiOBHm_Chr5g0074761
rosa_laevigata RLG00000036502
rosa_multiflora Rmu_sc0000909.1_g000018
rosa_roxburghii Rroxscaffold_1G00006820
rosa_rugosa Rorug05G0433700
rosa_samantha Rh5AG491400 Rh5BG512800 Rh5CG536500
rosa_wichuraiana Rw5G045510

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 535
Acc36I ACCTGC 1 cut(s) 51
Acc65I GGTACC 1 cut(s) 600
AccB1I GGYRCC 1 cut(s) 600
AccB7I CCANNNNNTGG 1 cut(s) 1379
AccI GTMKAC 1 cut(s) 1206
AciI CCGC 5 cut(s) 169, 1228, 1241, 1535, 1617
AcoI YGGCCR 1 cut(s) 1216
AcsI RAATTY 2 cut(s) 219, 1446
AcyI GRCGYC 1 cut(s) 1515
AfaI GTAC 5 cut(s) 506, 546, 602, 695, 1142
AfiI CCNNNNNNNGG 4 cut(s) 610, 1011, 1116, 1379
AflIII ACRYGT 3 cut(s) 501, 903, 1575
AgsI TTSAA 8 cut(s) 101, 361, 425, 516, 757, 929, 1187, 1351
AluBI AGCT 9 cut(s) 467, 489, 789, 818, 986, 1249, 1456, 1497, 1653
AluI AGCT 9 cut(s) 467, 489, 789, 818, 986, 1249, 1456, 1497, 1653
Alw21I GWGCWC 4 cut(s) 791, 808, 820, 1251
Alw26I GTCTC 4 cut(s) 27, 121, 217, 1472
Ama87I CYCGRG 1 cut(s) 508
AoxI GGCC 3 cut(s) 157, 456, 1216
ApeKI GCWGC 3 cut(s) 5, 108, 921
ApoI RAATTY 2 cut(s) 219, 1446
ArsI GACNNNNNNTTYG 2 cut(s) 736, 768
AseI ATTAAT 1 cut(s) 296
Asp700I GAANNNNTTC 2 cut(s) 945, 1028
Asp718I GGTACC 1 cut(s) 600
AspLEI GCGC 3 cut(s) 536, 680, 1307
AsuC2I CCSGG 2 cut(s) 1117, 1225
AsuHPI GGTGA 3 cut(s) 215, 1334, 1394
AsuII TTCGAA 1 cut(s) 1032
AvaI CYCGRG 1 cut(s) 508
AxyI CCTNAGG 1 cut(s) 1328
BaeI ACNNNNGTAYC 2 cut(s) 465, 498
BanI GGYRCC 1 cut(s) 600
BanII GRGCYC 3 cut(s) 791, 820, 1251
BauI CACGAG 1 cut(s) 1485
Bbv12I GWGCWC 4 cut(s) 791, 808, 820, 1251
BbvI GCAGC 2 cut(s) 95, 933
BccI CCATC 3 cut(s) 55, 603, 1505
BceAI ACGGC 1 cut(s) 1203
BcgI CGANNNNNNTGC 2 cut(s) 756, 790
BciVI GTATCC 5 cut(s) 163, 466, 831, 1077, 1127
BclI TGATCA 1 cut(s) 285
BcnI CCSGG 2 cut(s) 1117, 1225
BcoDI GTCTC 4 cut(s) 27, 121, 217, 1472
BfaI CTAG 6 cut(s) 350, 630, 782, 821, 1232, 1397
BfoI RGCGCY 1 cut(s) 1308
BfuAI ACCTGC 1 cut(s) 51
BfuI GTATCC 5 cut(s) 163, 466, 831, 1077, 1127
BisI GCNGC 4 cut(s) 6, 109, 922, 1229
BlsI GCNGC 4 cut(s) 7, 110, 923, 1230
BmcAI AGTACT 2 cut(s) 546, 1142
Bme1390I CCNGG 2 cut(s) 1117, 1225
BmeT110I CYCGRG 1 cut(s) 508
BmiI GGNNCC 3 cut(s) 602, 1340, 1371
BmrFI CCNGG 2 cut(s) 1117, 1225
BmsI GCATC 6 cut(s) 655, 677, 709, 958, 1006, 1187
BpmI CTGGAG 2 cut(s) 179, 1443
Bpu14I TTCGAA 1 cut(s) 1032
BpuMI CCSGG 2 cut(s) 1117, 1225
BsaHI GRCGYC 1 cut(s) 1515
Bsc4I CCNNNNNNNGG 4 cut(s) 610, 1011, 1116, 1379
Bse118I RCCGGY 2 cut(s) 182, 458
Bse1I ACTGG 3 cut(s) 196, 1371, 1426
Bse21I CCTNAGG 1 cut(s) 1328
Bse3DI GCAATG 3 cut(s) 439, 642, 687
BseGI GGATG 4 cut(s) 47, 1021, 1202, 1497
BseLI CCNNNNNNNGG 4 cut(s) 610, 1011, 1116, 1379
BseMI GCAATG 3 cut(s) 439, 642, 687
BseMII CTCAG 3 cut(s) 155, 552, 1319
BseNI ACTGG 3 cut(s) 196, 1371, 1426
BseRI GAGGAG 2 cut(s) 6, 1345
BseX3I CGGCCG 1 cut(s) 1216
BseXI GCAGC 2 cut(s) 95, 933
Bsh1285I CGRYCG 1 cut(s) 1219
BshFI GGCC 3 cut(s) 159, 458, 1218
BshNI GGYRCC 1 cut(s) 600
BsiEI CGRYCG 1 cut(s) 1219
BsiHKAI GWGCWC 4 cut(s) 791, 808, 820, 1251
BsiHKCI CYCGRG 1 cut(s) 508
BsiSI CCGG 7 cut(s) 156, 183, 445, 459, 1116, 1224, 1265
BslFI GGGAC 2 cut(s) 295, 1595
BslI CCNNNNNNNGG 4 cut(s) 610, 1011, 1116, 1379
BsmAI GTCTC 4 cut(s) 27, 121, 217, 1472
BsmBI CGTCTC 1 cut(s) 27
BsmFI GGGAC 2 cut(s) 295, 1595
BsmI GAATGC 3 cut(s) 81, 418, 1346
BsnI GGCC 3 cut(s) 159, 458, 1218
BsoBI CYCGRG 1 cut(s) 508
Bsp119I TTCGAA 1 cut(s) 1032
Bsp1286I GDGCHC 4 cut(s) 791, 808, 820, 1251
Bsp143I GATC 2 cut(s) 285, 823
BspACI CCGC 5 cut(s) 169, 1228, 1241, 1535, 1617
BspANI GGCC 3 cut(s) 159, 458, 1218
BspCNI CTCAG 3 cut(s) 154, 553, 1320
BspLI GGNNCC 3 cut(s) 602, 1340, 1371
BspMI ACCTGC 1 cut(s) 51
BspQI GCTCTTC 1 cut(s) 1239
BspT104I TTCGAA 1 cut(s) 1032
BspT107I GGYRCC 1 cut(s) 600
BsrDI GCAATG 3 cut(s) 439, 642, 687
BsrFI RCCGGY 2 cut(s) 182, 458
BsrI ACTGG 3 cut(s) 196, 1371, 1426
BssAI RCCGGY 2 cut(s) 182, 458
BssMI GATC 2 cut(s) 285, 823
BssNI GRCGYC 1 cut(s) 1515
BssSI CACGAG 1 cut(s) 1485
Bst2BI CACGAG 1 cut(s) 1485
Bst4CI ACNGT 6 cut(s) 70, 524, 707, 935, 1038, 1179
Bst6I CTCTTC 3 cut(s) 30, 1230, 1239
BstACI GRCGYC 1 cut(s) 1515
BstBI TTCGAA 1 cut(s) 1032
BstC8I GCNNGC 4 cut(s) 138, 460, 576, 1556
BstDEI CTNAG 5 cut(s) 141, 561, 605, 867, 1328
BstEII GGTNACC 1 cut(s) 1322
BstF5I GGATG 4 cut(s) 47, 1021, 1202, 1497
BstH2I RGCGCY 1 cut(s) 1308
BstHHI GCGC 3 cut(s) 536, 680, 1307
BstKTI GATC 2 cut(s) 288, 826
BstMAI GTCTC 4 cut(s) 27, 121, 217, 1472
BstMBI GATC 2 cut(s) 285, 823
BstMCI CGRYCG 1 cut(s) 1219
BstMWI GCNNNNNNNGC 2 cut(s) 11, 464
BstNSI RCATGY 6 cut(s) 140, 402, 505, 907, 1579, 1645
BstPI GGTNACC 1 cut(s) 1322
BstSCI CCNGG 2 cut(s) 1115, 1223
BstV1I GCAGC 2 cut(s) 95, 933
BstZI CGGCCG 1 cut(s) 1216
Bsu36I CCTNAGG 1 cut(s) 1328
BsuI GTATCC 5 cut(s) 163, 466, 831, 1077, 1127
BsuRI GGCC 3 cut(s) 159, 458, 1218
BtsCI GGATG 4 cut(s) 47, 1021, 1202, 1497
BtsI GCAGTG 1 cut(s) 888
BtsIMutI CAGTG 2 cut(s) 75, 888
BveI ACCTGC 1 cut(s) 51
Cac8I GCNNGC 4 cut(s) 138, 460, 576, 1556
CfoI GCGC 3 cut(s) 536, 680, 1307
Cfr10I RCCGGY 2 cut(s) 182, 458
CseI GACGC 2 cut(s) 1324, 1504
Csp6I GTAC 5 cut(s) 505, 545, 601, 694, 1141
CviQI GTAC 5 cut(s) 505, 545, 601, 694, 1141
DdeI CTNAG 5 cut(s) 141, 561, 605, 867, 1328
DpnI GATC 2 cut(s) 287, 825
DpnII GATC 2 cut(s) 285, 823
EaeI YGGCCR 1 cut(s) 1216
EagI CGGCCG 1 cut(s) 1216
Eam1104I CTCTTC 3 cut(s) 30, 1230, 1239
EarI CTCTTC 3 cut(s) 30, 1230, 1239
EciI GGCGGA 1 cut(s) 1256
Ecl136II GAGCTC 3 cut(s) 789, 818, 1249
EclXI CGGCCG 1 cut(s) 1216
Eco24I GRGCYC 3 cut(s) 791, 820, 1251
Eco52I CGGCCG 1 cut(s) 1216
Eco53kI GAGCTC 3 cut(s) 789, 818, 1249
Eco81I CCTNAGG 1 cut(s) 1328
Eco88I CYCGRG 1 cut(s) 508
Eco91I GGTNACC 1 cut(s) 1322
EcoICRI GAGCTC 3 cut(s) 789, 818, 1249
EcoO65I GGTNACC 1 cut(s) 1322
EcoT22I ATGCAT 3 cut(s) 670, 724, 1202
EcoT38I GRGCYC 3 cut(s) 791, 820, 1251
Esp3I CGTCTC 1 cut(s) 27
FalI AAGNNNNNCTT 2 cut(s) 572, 604
FaqI GGGAC 2 cut(s) 295, 1595
FauI CCCGC 1 cut(s) 1528
FauNDI CATATG 1 cut(s) 847
FbaI TGATCA 1 cut(s) 285
FblI GTMKAC 1 cut(s) 1206
Fnu4HI GCNGC 4 cut(s) 6, 109, 922, 1229
FokI GGATG 4 cut(s) 34, 1028, 1209, 1504
FriOI GRGCYC 3 cut(s) 791, 820, 1251
Fsp4HI GCNGC 4 cut(s) 6, 109, 922, 1229
FspBI CTAG 6 cut(s) 350, 630, 782, 821, 1232, 1397
FspI TGCGCA 1 cut(s) 535
GlaI GCGC 3 cut(s) 535, 679, 1306
GluI GCNGC 4 cut(s) 6, 109, 922, 1229
GsuI CTGGAG 2 cut(s) 179, 1443
HaeII RGCGCY 1 cut(s) 1308
HaeIII GGCC 3 cut(s) 159, 458, 1218
HapII CCGG 7 cut(s) 156, 183, 445, 459, 1116, 1224, 1265
HgaI GACGC 2 cut(s) 1324, 1504
HhaI GCGC 3 cut(s) 536, 680, 1307
Hin1I GRCGYC 1 cut(s) 1515
Hin6I GCGC 3 cut(s) 534, 678, 1305
HinP1I GCGC 3 cut(s) 534, 678, 1305
HinfI GANTC 2 cut(s) 773, 880
HpaII CCGG 7 cut(s) 156, 183, 445, 459, 1116, 1224, 1265
HphI GGTGA 3 cut(s) 215, 1334, 1394
Hpy166II GTNNAC 3 cut(s) 484, 696, 1207
Hpy188III TCNNGA 2 cut(s) 260, 821
Hpy8I GTNNAC 3 cut(s) 484, 696, 1207
Hpy99I CGWCG 1 cut(s) 1520
HpyAV CCTTC 5 cut(s) 61, 419, 619, 751, 1220
HpyCH4III ACNGT 6 cut(s) 70, 524, 707, 935, 1038, 1179
HpyCH4IV ACGT 1 cut(s) 480
HpyF10VI GCNNNNNNNGC 2 cut(s) 11, 464
HpyF3I CTNAG 5 cut(s) 141, 561, 605, 867, 1328
HpySE526I ACGT 1 cut(s) 480
Hsp92I GRCGYC 1 cut(s) 1515
HspAI GCGC 3 cut(s) 534, 678, 1305
KpnI GGTACC 1 cut(s) 604
KroI GCCGGC 1 cut(s) 458
KroNI GCCGGC 1 cut(s) 460
Ksp22I TGATCA 1 cut(s) 285
Kzo9I GATC 2 cut(s) 285, 823
LguI GCTCTTC 1 cut(s) 1239
LmnI GCTCC 4 cut(s) 19, 675, 815, 983
Lsp1109I GCAGC 2 cut(s) 95, 933
LweI GCATC 6 cut(s) 655, 677, 709, 958, 1006, 1187
MaeI CTAG 6 cut(s) 350, 630, 782, 821, 1232, 1397
MaeII ACGT 1 cut(s) 480
MaeIII GTNAC 6 cut(s) 877, 935, 957, 973, 1322, 1382
MalI GATC 2 cut(s) 287, 825
MboI GATC 2 cut(s) 285, 823
MboII GAAGA 4 cut(s) 17, 964, 1247, 1256
MhlI GDGCHC 4 cut(s) 791, 808, 820, 1251
MluCI AATT 7 cut(s) 219, 297, 370, 591, 1276, 1446, 1472
MlyI GAGTC 1 cut(s) 874
MmeI TCCRAC 1 cut(s) 1292
Mph1103I ATGCAT 3 cut(s) 670, 724, 1202
MroNI GCCGGC 1 cut(s) 458
MroXI GAANNNNTTC 2 cut(s) 945, 1028
MseI TTAA 6 cut(s) 296, 306, 393, 453, 590, 1499
MslI CAYNNNNRTG 6 cut(s) 846, 902, 1014, 1085, 1164, 1259
MspI CCGG 7 cut(s) 156, 183, 445, 459, 1116, 1224, 1265
MspR9I CCNGG 2 cut(s) 1117, 1225
Mva1269I GAATGC 3 cut(s) 81, 418, 1346
MwoI GCNNNNNNNGC 2 cut(s) 11, 464
NaeI GCCGGC 1 cut(s) 460
NciI CCSGG 2 cut(s) 1117, 1225
NdeI CATATG 1 cut(s) 847
NdeII GATC 2 cut(s) 285, 823
NgoMIV GCCGGC 1 cut(s) 458
NlaIV GGNNCC 3 cut(s) 602, 1340, 1371
NmeAIII GCCGAG 2 cut(s) 1045, 1194
NmuCI GTSAC 4 cut(s) 877, 957, 1322, 1382
NsbI TGCGCA 1 cut(s) 535
NsiI ATGCAT 3 cut(s) 670, 724, 1202
NspI RCATGY 6 cut(s) 140, 402, 505, 907, 1579, 1645
NspV TTCGAA 1 cut(s) 1032
PaeI GCATGC 1 cut(s) 140
PaeR7I CTCGAG 1 cut(s) 508
PciI ACATGT 3 cut(s) 501, 903, 1575
PciSI GCTCTTC 1 cut(s) 1239
PcsI WCGNNNNNNNCGW 1 cut(s) 1467
PctI GAATGC 3 cut(s) 81, 418, 1346
PdiI GCCGGC 1 cut(s) 460
PdmI GAANNNNTTC 2 cut(s) 945, 1028
PfeI GAWTC 1 cut(s) 773
PflMI CCANNNNNTGG 1 cut(s) 1379
PkrI GCNGC 4 cut(s) 7, 110, 923, 1230
PleI GAGTC 1 cut(s) 874
PpsI GAGTC 1 cut(s) 874
PscI ACATGT 3 cut(s) 501, 903, 1575
PshBI ATTAAT 1 cut(s) 296
Psp124BI GAGCTC 3 cut(s) 791, 820, 1251
PspEI GGTNACC 1 cut(s) 1322
PspN4I GGNNCC 3 cut(s) 602, 1340, 1371
PspXI VCTCGAGB 1 cut(s) 508
RsaI GTAC 5 cut(s) 506, 546, 602, 695, 1142
RsaNI GTAC 5 cut(s) 505, 545, 601, 694, 1141
RseI CAYNNNNRTG 6 cut(s) 846, 902, 1014, 1085, 1164, 1259
SacI GAGCTC 3 cut(s) 791, 820, 1251
SapI GCTCTTC 1 cut(s) 1239
SaqAI TTAA 6 cut(s) 296, 306, 393, 453, 590, 1499
SatI GCNGC 4 cut(s) 6, 109, 922, 1229
Sau3AI GATC 2 cut(s) 285, 823
ScaI AGTACT 2 cut(s) 546, 1142
SchI GAGTC 1 cut(s) 874
ScrFI CCNGG 2 cut(s) 1117, 1225
SduI GDGCHC 4 cut(s) 791, 808, 820, 1251
SfaNI GCATC 6 cut(s) 655, 677, 709, 958, 1006, 1187
Sfr274I CTCGAG 1 cut(s) 508
SfuI TTCGAA 1 cut(s) 1032
SlaI CTCGAG 1 cut(s) 508
SmiMI CAYNNNNRTG 6 cut(s) 846, 902, 1014, 1085, 1164, 1259
SmlI CTYRAG 1 cut(s) 508
SmoI CTYRAG 1 cut(s) 508
SphI GCATGC 1 cut(s) 140
Sse9I AATT 7 cut(s) 219, 297, 370, 591, 1276, 1446, 1472
SsiI CCGC 5 cut(s) 169, 1228, 1241, 1535, 1617
SspMI CTAG 6 cut(s) 350, 630, 782, 821, 1232, 1397
SstI GAGCTC 3 cut(s) 791, 820, 1251
StyD4I CCNGG 2 cut(s) 1115, 1223
TaaI ACNGT 6 cut(s) 70, 524, 707, 935, 1038, 1179
TaiI ACGT 1 cut(s) 483
TaqI TCGA 7 cut(s) 236, 243, 509, 776, 1032, 1125, 1503
TasI AATT 7 cut(s) 219, 297, 370, 591, 1276, 1446, 1472
TatI WGTACW 3 cut(s) 504, 544, 1140
TauI GCSGC 1 cut(s) 1231
TfiI GAWTC 1 cut(s) 773
Tru1I TTAA 6 cut(s) 296, 306, 393, 453, 590, 1499
Tru9I TTAA 6 cut(s) 296, 306, 393, 453, 590, 1499
TscAI CASTG 2 cut(s) 75, 895
TseFI GTSAC 4 cut(s) 877, 957, 1322, 1382
TseI GCWGC 3 cut(s) 5, 108, 921
Tsp45I GTSAC 4 cut(s) 877, 957, 1322, 1382
TspDTI ATGAA 4 cut(s) 1059, 1535, 1580, 1626
TspGWI ACGGA 2 cut(s) 9, 1074
TspRI CASTG 2 cut(s) 75, 895
Van91I CCANNNNNTGG 1 cut(s) 1379
VspI ATTAAT 1 cut(s) 296
XapI RAATTY 2 cut(s) 219, 1446
XbaI TCTAGA 1 cut(s) 820
XceI RCATGY 6 cut(s) 140, 402, 505, 907, 1579, 1645
XhoI CTCGAG 1 cut(s) 508
XmiI GTMKAC 1 cut(s) 1206
XmnI GAANNNNTTC 2 cut(s) 945, 1028
XspI CTAG 6 cut(s) 350, 630, 782, 821, 1232, 1397
ZrmI AGTACT 2 cut(s) 546, 1142
Zsp2I ATGCAT 3 cut(s) 670, 724, 1202
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.