Rw5G045510

PPR repeat

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0013877
Physical Location & Seq
Reverse (-)
79525331 .. 79526965
1635 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G045510.1

Sequence Viewer

Length: 1635 bp
ATGGCTGCTGTTGCTCCTCCATCACTTCCCAACCAGAACAAATTACCATCCTTCTCGTCGAGAATTCTAGCTGGAATGCCGGCCCCCGTCGTTTTGCTTCAAATGTGCAGCAACTTTCAAGAAGTAAGACAAGTTCATGCTCAATTGGTTGTATCAGGACTAATTGAGCGCCCTATAAATGGTGGGAGGCTTCTTCAGGCCTATGTCAGAGTGTCAAACTTTAACTATGCATTATCAACTTTTGATAGCATATCTAATCCTGATGTGTTTGCCTACAATACCATGATGAGAGGTTTAATACTGGGTAAGTCCCCTTATCATGCACTGAAGCTCTATAGTAGGCTTTTACTAGATGGTTTAAAACCAGACAATTACACCTACACCTTTGTTCTCAAAGCATGTTCCCAAGTGAATGCGCTCTTCGAAGGTAGAGAAGTGCATGGCCGGATAATTAAGGCCGGAATAGCACCAGATACTTACATTCATAGTTCACTGATAAACATGTACTCGAGTTCATCAGATGGTTTGGTCTGTGCAGAGCGAGTTTTAGCTGGCTTTTCCGAGGATAACACACTTGCTAAGAACTCCTTGATTTCGGGTTACCTCAGTCAGGGTCAGGTAGAAAGTGCTAGAAAAATGTTCGATAACCTGGTAGCAAAGGATGATGCATCTTGGAGTGCAATGGTAGCAGGGTACACAAAGAATTGTATGTATGCAGAGGCATTAGTCATCTTTCGAAAGATGTTGAGTTCTCAAGTTTCTCCAAATGAATCAGCACTTGTGAGCTCATTGTGTGCTTGTGCTAATTTGGGAGCACTAGACCAAGGCAAATGGATACATGCATACATTGATAGAATTGGGGCTAGGATTAGTGTCACTGTGGGTACTGCTCTTATAGACATGTATGCAAAGTGTGGCAGCATAGAAAATGCCTATGAAGTTTTCAGGAAGATGCCACAGAAAGACGTAGTTTCATGGGGAGCTATCATTTCTGGTTTCGCCATTCATGGGCGAGCTGCAAAATGTTTTGAATTGTTTGATGATATGGTTGCTGGTGGAATTCACCCAAATGAAGTAATTTTTGTAGGTATTTTATCGGCTTGTTCTCATGCCGGGTATGTTGAAACAGGATACAAGTACTTTGACCAAATGGTTTATCATTATGGGATAAAACCATCCATTGAACATTATGGATGCATGGTGGATCTTCTTGGTCGTGCAGGGCGGCTGGCAGAGGCAGAAGGGTTCATCATGTCAATGCCGGAACAGCCTAACTCGGTTATATGGGGTGCATTTCTTGGTGCTTGTAGAATGTACAGTGACGTGAGGAGAGGTAACCTTGCTTTCAAGCACTTGACTGATCTTGAACCAACGTCTGGTGACAGATACAAACTTGCAGGAATTATGTTTGCTAATGCTGGGGAAAAGGAAAATGCTACTAAAATTAGGACGATCATCACAGATAATGACTTGGAGACAACTCGTGGAGTAAGCTCAATTGAGATAGATGGTGTAGTCCACGAGTTTGTAGCAGGTACCATCAACCATAGCAGGCACAAAGAAATTTACAGAATGTGGGAGAGGGTTAATAGGTTATTAGAAATCCATGAGCTTGAAATTTGCATTCCATTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PPR PPR_2

Protein Analysis

544

Amino Acids

60.01

Weight (kDa)

6.73

Isoelectric Point (pI)

40.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 87 - 135 1.4e-09 PPR repeat family
PPR_2 PF13041 321 - 369 8.2e-08 PPR repeat family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013877)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G28280 AT4G28280 AT4G28280
malus_domestica MD03G1042100.v1.1 MD11G1044400.v1.1 MD11G1045000.v1.1
prunus_persica Prupe.6G034000_v2.0.a1
pyrus_communis pycom03g03260
rosa_chinensis RchiOBHm_Chr5g0074761
rosa_laevigata RLG00000036502
rosa_multiflora Rmu_sc0000909.1_g000018
rosa_roxburghii Rroxscaffold_1G00006820
rosa_rugosa Rorug05G0433700
rosa_samantha Rh5AG491400 Rh5BG512800 Rh5CG536500
rosa_wichuraiana Rw5G045510

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1523
Acc65I GGTACC 1 cut(s) 1535
AccB1I GGYRCC 1 cut(s) 1535
AccB7I CCANNNNNTGG 1 cut(s) 1376
AciI CCGC 1 cut(s) 1225
AclWI GGATC 1 cut(s) 1212
AcoI YGGCCR 1 cut(s) 442
AcsI RAATTY 4 cut(s) 63, 1059, 1563, 1617
AcuI CTGAAG 2 cut(s) 179, 347
AfaI GTAC 6 cut(s) 506, 695, 886, 1139, 1316, 1537
AfiI CCNNNNNNNGG 4 cut(s) 179, 610, 1008, 1376
AflIII ACRYGT 2 cut(s) 501, 900
AgsI TTSAA 8 cut(s) 101, 119, 1031, 1124, 1184, 1348, 1367, 1616
AjiI CACGTC 1 cut(s) 1324
AjnI CCWGG 1 cut(s) 648
AluBI AGCT 8 cut(s) 71, 331, 551, 786, 983, 1016, 1494, 1612
AluI AGCT 8 cut(s) 71, 331, 551, 786, 983, 1016, 1494, 1612
Alw21I GWGCWC 2 cut(s) 788, 817
Alw26I GTCTC 1 cut(s) 1469
AlwI GGATC 1 cut(s) 1212
Ama87I CYCGRG 1 cut(s) 508
AoxI GGCC 4 cut(s) 81, 198, 442, 456
ApeKI GCWGC 4 cut(s) 5, 108, 918, 1016
ApoI RAATTY 4 cut(s) 63, 1059, 1563, 1617
Asp718I GGTACC 1 cut(s) 1535
AspLEI GCGC 2 cut(s) 171, 418
AspS9I GGNCC 1 cut(s) 82
AsuC2I CCSGG 1 cut(s) 1114
AsuHPI GGTGA 2 cut(s) 1055, 1391
AsuII TTCGAA 2 cut(s) 423, 736
AvaI CYCGRG 1 cut(s) 508
BanI GGYRCC 1 cut(s) 1535
BanII GRGCYC 1 cut(s) 788
BauI CACGAG 2 cut(s) 1482, 1520
Bbv12I GWGCWC 2 cut(s) 788, 817
BbvI GCAGC 3 cut(s) 120, 930, 1003
BccI CCATC 7 cut(s) 28, 55, 347, 515, 1183, 1502, 1547
BciT130I CCWGG 1 cut(s) 650
BciVI GTATCC 2 cut(s) 828, 1124
BcnI CCSGG 1 cut(s) 1114
BcoDI GTCTC 1 cut(s) 1469
BfaI CTAG 6 cut(s) 68, 350, 630, 818, 864, 1633
BfmI CTRYAG 1 cut(s) 334
BfoI RGCGCY 1 cut(s) 172
BfuAI ACCTGC 1 cut(s) 1523
BfuI GTATCC 2 cut(s) 828, 1124
BisI GCNGC 5 cut(s) 6, 109, 919, 1017, 1226
BlsI GCNGC 5 cut(s) 7, 110, 920, 1018, 1227
BmcAI AGTACT 1 cut(s) 1139
Bme1390I CCNGG 2 cut(s) 650, 1114
BmeT110I CYCGRG 1 cut(s) 508
BmgBI CACGTC 1 cut(s) 1324
BmgT120I GGNCC 1 cut(s) 82
BmiI GGNNCC 2 cut(s) 84, 1537
BmrFI CCNGG 2 cut(s) 650, 1114
BmrI ACTGGG 1 cut(s) 311
BmsI GCATC 4 cut(s) 655, 677, 942, 1184
BmuI ACTGGG 1 cut(s) 311
Bpu14I TTCGAA 2 cut(s) 423, 736
BpuEI CTTGAG 1 cut(s) 738
BpuMI CCSGG 1 cut(s) 1114
BsaJI CCNNGG 2 cut(s) 561, 823
Bsc4I CCNNNNNNNGG 4 cut(s) 179, 610, 1008, 1376
Bse118I RCCGGY 1 cut(s) 79
Bse1I ACTGG 1 cut(s) 306
Bse3DI GCAATG 1 cut(s) 687
BseBI CCWGG 1 cut(s) 650
BseDI CCNNGG 2 cut(s) 561, 823
BseGI GGATG 4 cut(s) 47, 667, 1175, 1199
BseLI CCNNNNNNNGG 4 cut(s) 179, 610, 1008, 1376
BseMI GCAATG 1 cut(s) 687
BseMII CTCAG 1 cut(s) 619
BseNI ACTGG 1 cut(s) 306
BseRI GAGGAG 2 cut(s) 6, 1342
BseXI GCAGC 3 cut(s) 120, 930, 1003
BseYI CCCAGC 1 cut(s) 1418
BsgI GTGCAG 3 cut(s) 127, 555, 1239
BshFI GGCC 4 cut(s) 83, 200, 444, 458
BshNI GGYRCC 1 cut(s) 1535
BsiHKAI GWGCWC 2 cut(s) 788, 817
BsiHKCI CYCGRG 1 cut(s) 508
BsiSI CCGG 5 cut(s) 80, 445, 459, 1113, 1262
BslFI GGGAC 1 cut(s) 295
BslI CCNNNNNNNGG 4 cut(s) 179, 610, 1008, 1376
BsmAI GTCTC 1 cut(s) 1469
BsmFI GGGAC 1 cut(s) 295
BsmI GAATGC 3 cut(s) 81, 418, 1623
BsnI GGCC 4 cut(s) 83, 200, 444, 458
BsoBI CYCGRG 1 cut(s) 508
Bsp119I TTCGAA 2 cut(s) 423, 736
Bsp1286I GDGCHC 2 cut(s) 788, 817
Bsp1407I TGTACA 1 cut(s) 1314
Bsp143I GATC 3 cut(s) 1204, 1360, 1452
BspACI CCGC 1 cut(s) 1225
BspANI GGCC 4 cut(s) 83, 200, 444, 458
BspCNI CTCAG 1 cut(s) 618
BspLI GGNNCC 2 cut(s) 84, 1537
BspMI ACCTGC 1 cut(s) 1523
BspPI GGATC 1 cut(s) 1212
BspQI GCTCTTC 1 cut(s) 425
BspT104I TTCGAA 2 cut(s) 423, 736
BspT107I GGYRCC 1 cut(s) 1535
BsrDI GCAATG 1 cut(s) 687
BsrFI RCCGGY 1 cut(s) 79
BsrGI TGTACA 1 cut(s) 1314
BsrI ACTGG 1 cut(s) 306
BssAI RCCGGY 1 cut(s) 79
BssECI CCNNGG 2 cut(s) 561, 823
BssMI GATC 3 cut(s) 1204, 1360, 1452
BssSI CACGAG 2 cut(s) 1482, 1520
BssT1I CCWWGG 1 cut(s) 823
Bst2BI CACGAG 2 cut(s) 1482, 1520
Bst2UI CCWGG 1 cut(s) 650
Bst4CI ACNGT 2 cut(s) 880, 1319
Bst6I CTCTTC 1 cut(s) 425
BstAUI TGTACA 1 cut(s) 1314
BstBI TTCGAA 2 cut(s) 423, 736
BstC8I GCNNGC 5 cut(s) 81, 553, 1014, 1230, 1553
BstDEI CTNAG 2 cut(s) 579, 605
BstEII GGTNACC 2 cut(s) 599, 1334
BstENI CCTNNNNNAGG 1 cut(s) 608
BstF5I GGATG 4 cut(s) 47, 667, 1175, 1199
BstH2I RGCGCY 1 cut(s) 172
BstHHI GCGC 2 cut(s) 171, 418
BstKTI GATC 3 cut(s) 1207, 1363, 1455
BstMAI GTCTC 1 cut(s) 1469
BstMBI GATC 3 cut(s) 1204, 1360, 1452
BstMWI GCNNNNNNNGC 4 cut(s) 11, 464, 686, 1267
BstNI CCWGG 1 cut(s) 650
BstNSI RCATGY 4 cut(s) 402, 505, 842, 904
BstPI GGTNACC 2 cut(s) 599, 1334
BstSCI CCNGG 2 cut(s) 648, 1112
BstSFI CTRYAG 1 cut(s) 334
BstV1I GCAGC 3 cut(s) 120, 930, 1003
BstX2I RGATCY 1 cut(s) 1204
BstYI RGATCY 1 cut(s) 1204
BsuI GTATCC 2 cut(s) 828, 1124
BsuRI GGCC 4 cut(s) 83, 200, 444, 458
BtrI CACGTC 1 cut(s) 1324
BtsCI GGATG 4 cut(s) 47, 667, 1175, 1199
BtsIMutI CAGTG 4 cut(s) 323, 491, 876, 1324
BveI ACCTGC 1 cut(s) 1523
Cac8I GCNNGC 5 cut(s) 81, 553, 1014, 1230, 1553
CfoI GCGC 2 cut(s) 171, 418
Cfr10I RCCGGY 1 cut(s) 79
Cfr13I GGNCC 1 cut(s) 82
CsiI ACCWGGT 1 cut(s) 648
Csp6I GTAC 6 cut(s) 505, 694, 885, 1138, 1315, 1536
CviQI GTAC 6 cut(s) 505, 694, 885, 1138, 1315, 1536
DdeI CTNAG 2 cut(s) 579, 605
DpnI GATC 3 cut(s) 1206, 1362, 1454
DpnII GATC 3 cut(s) 1204, 1360, 1452
DraI TTTAAA 1 cut(s) 360
EaeI YGGCCR 1 cut(s) 442
Eam1104I CTCTTC 1 cut(s) 425
EarI CTCTTC 1 cut(s) 425
Ecl136II GAGCTC 1 cut(s) 786
Eco130I CCWWGG 1 cut(s) 823
Eco147I AGGCCT 1 cut(s) 200
Eco24I GRGCYC 1 cut(s) 788
Eco53kI GAGCTC 1 cut(s) 786
Eco57I CTGAAG 2 cut(s) 179, 347
Eco88I CYCGRG 1 cut(s) 508
Eco91I GGTNACC 2 cut(s) 599, 1334
EcoICRI GAGCTC 1 cut(s) 786
EcoNI CCTNNNNNAGG 1 cut(s) 608
EcoO65I GGTNACC 2 cut(s) 599, 1334
EcoRI GAATTC 2 cut(s) 63, 1059
EcoRII CCWGG 1 cut(s) 648
EcoT14I CCWWGG 1 cut(s) 823
EcoT22I ATGCAT 4 cut(s) 232, 670, 844, 1199
EcoT38I GRGCYC 1 cut(s) 788
ErhI CCWWGG 1 cut(s) 823
FalI AAGNNNNNCTT 2 cut(s) 572, 604
FaqI GGGAC 1 cut(s) 295
Fnu4HI GCNGC 5 cut(s) 6, 109, 919, 1017, 1226
FokI GGATG 4 cut(s) 34, 674, 1162, 1206
FriOI GRGCYC 1 cut(s) 788
Fsp4HI GCNGC 5 cut(s) 6, 109, 919, 1017, 1226
FspBI CTAG 6 cut(s) 68, 350, 630, 818, 864, 1633
GlaI GCGC 2 cut(s) 170, 417
GluI GCNGC 5 cut(s) 6, 109, 919, 1017, 1226
GsaI CCCAGC 1 cut(s) 1422
HaeII RGCGCY 1 cut(s) 172
HaeIII GGCC 4 cut(s) 83, 200, 444, 458
HapII CCGG 5 cut(s) 80, 445, 459, 1113, 1262
HhaI GCGC 2 cut(s) 171, 418
Hin6I GCGC 2 cut(s) 169, 416
HinP1I GCGC 2 cut(s) 169, 416
HinfI GANTC 1 cut(s) 770
HpaII CCGG 5 cut(s) 80, 445, 459, 1113, 1262
HphI GGTGA 2 cut(s) 1055, 1391
Hpy166II GTNNAC 3 cut(s) 491, 696, 1519
Hpy188I TCNGA 3 cut(s) 209, 520, 562
Hpy188III TCNNGA 6 cut(s) 60, 119, 156, 260, 946, 1364
Hpy8I GTNNAC 3 cut(s) 491, 696, 1519
Hpy99I CGWCG 2 cut(s) 61, 92
HpyAV CCTTC 3 cut(s) 61, 419, 1235
HpyCH4III ACNGT 2 cut(s) 880, 1319
HpyCH4IV ACGT 3 cut(s) 966, 1323, 1373
HpyF10VI GCNNNNNNNGC 4 cut(s) 11, 464, 686, 1267
HpyF3I CTNAG 2 cut(s) 579, 605
HpySE526I ACGT 3 cut(s) 966, 1323, 1373
HspAI GCGC 2 cut(s) 169, 416
KpnI GGTACC 1 cut(s) 1539
KroI GCCGGC 1 cut(s) 79
KroNI GCCGGC 1 cut(s) 81
Kzo9I GATC 3 cut(s) 1204, 1360, 1452
LguI GCTCTTC 1 cut(s) 425
LmnI GCTCC 3 cut(s) 19, 812, 980
Lsp1109I GCAGC 3 cut(s) 120, 930, 1003
LweI GCATC 4 cut(s) 655, 677, 942, 1184
MabI ACCWGGT 1 cut(s) 648
MaeI CTAG 6 cut(s) 68, 350, 630, 818, 864, 1633
MaeII ACGT 3 cut(s) 966, 1323, 1373
MaeIII GTNAC 5 cut(s) 599, 874, 1319, 1334, 1379
MalI GATC 3 cut(s) 1206, 1362, 1454
MboI GATC 3 cut(s) 1204, 1360, 1452
MboII GAAGA 4 cut(s) 185, 412, 961, 1199
MfeI CAATTG 2 cut(s) 143, 1497
MflI RGATCY 1 cut(s) 1204
MhlI GDGCHC 2 cut(s) 788, 817
Mph1103I ATGCAT 4 cut(s) 232, 670, 844, 1199
MroNI GCCGGC 1 cut(s) 79
MseI TTAA 5 cut(s) 222, 296, 359, 453, 1587
MslI CAYNNNNRTG 2 cut(s) 1068, 1256
MspI CCGG 5 cut(s) 80, 445, 459, 1113, 1262
MspR9I CCNGG 2 cut(s) 650, 1114
MunI CAATTG 2 cut(s) 143, 1497
Mva1269I GAATGC 3 cut(s) 81, 418, 1623
MvaI CCWGG 1 cut(s) 650
MwoI GCNNNNNNNGC 4 cut(s) 11, 464, 686, 1267
NaeI GCCGGC 1 cut(s) 81
NciI CCSGG 1 cut(s) 1114
NdeII GATC 3 cut(s) 1204, 1360, 1452
NgoMIV GCCGGC 1 cut(s) 79
NlaIV GGNNCC 2 cut(s) 84, 1537
NmuCI GTSAC 3 cut(s) 874, 1319, 1379
NsiI ATGCAT 4 cut(s) 232, 670, 844, 1199
NspI RCATGY 4 cut(s) 402, 505, 842, 904
NspV TTCGAA 2 cut(s) 423, 736
PaeR7I CTCGAG 1 cut(s) 508
PceI AGGCCT 1 cut(s) 200
PciI ACATGT 2 cut(s) 501, 900
PciSI GCTCTTC 1 cut(s) 425
PctI GAATGC 3 cut(s) 81, 418, 1623
PdiI GCCGGC 1 cut(s) 81
PfeI GAWTC 1 cut(s) 770
PflMI CCANNNNNTGG 1 cut(s) 1376
PkrI GCNGC 5 cut(s) 7, 110, 920, 1018, 1227
PscI ACATGT 2 cut(s) 501, 900
Psp124BI GAGCTC 1 cut(s) 788
Psp6I CCWGG 1 cut(s) 648
PspEI GGTNACC 2 cut(s) 599, 1334
PspFI CCCAGC 1 cut(s) 1418
PspGI CCWGG 1 cut(s) 648
PspN4I GGNNCC 2 cut(s) 84, 1537
PspPI GGNCC 1 cut(s) 82
PspXI VCTCGAGB 1 cut(s) 508
PsrI GAACNNNNNNTAC 2 cut(s) 117, 149
PsuI RGATCY 1 cut(s) 1204
RsaI GTAC 6 cut(s) 506, 695, 886, 1139, 1316, 1537
RsaNI GTAC 6 cut(s) 505, 694, 885, 1138, 1315, 1536
RseI CAYNNNNRTG 2 cut(s) 1068, 1256
SacI GAGCTC 1 cut(s) 788
SapI GCTCTTC 1 cut(s) 425
SaqAI TTAA 5 cut(s) 222, 296, 359, 453, 1587
SatI GCNGC 5 cut(s) 6, 109, 919, 1017, 1226
Sau3AI GATC 3 cut(s) 1204, 1360, 1452
Sau96I GGNCC 1 cut(s) 82
ScaI AGTACT 1 cut(s) 1139
ScrFI CCNGG 2 cut(s) 650, 1114
SduI GDGCHC 2 cut(s) 788, 817
SexAI ACCWGGT 1 cut(s) 648
SfaNI GCATC 4 cut(s) 655, 677, 942, 1184
SfcI CTRYAG 1 cut(s) 334
Sfr274I CTCGAG 1 cut(s) 508
SfuI TTCGAA 2 cut(s) 423, 736
SlaI CTCGAG 1 cut(s) 508
SmiMI CAYNNNNRTG 2 cut(s) 1068, 1256
SmlI CTYRAG 2 cut(s) 508, 753
SmoI CTYRAG 2 cut(s) 508, 753
SseBI AGGCCT 1 cut(s) 200
SsiI CCGC 1 cut(s) 1225
SspMI CTAG 6 cut(s) 68, 350, 630, 818, 864, 1633
SstI GAGCTC 1 cut(s) 788
StuI AGGCCT 1 cut(s) 200
StyD4I CCNGG 2 cut(s) 648, 1112
StyI CCWWGG 1 cut(s) 823
TaaI ACNGT 2 cut(s) 880, 1319
TaiI ACGT 3 cut(s) 969, 1326, 1376
TaqI TCGA 5 cut(s) 59, 423, 509, 642, 736
TatI WGTACW 3 cut(s) 504, 1137, 1314
TauI GCSGC 1 cut(s) 1228
TfiI GAWTC 1 cut(s) 770
Tru1I TTAA 5 cut(s) 222, 296, 359, 453, 1587
Tru9I TTAA 5 cut(s) 222, 296, 359, 453, 1587
TscAI CASTG 4 cut(s) 330, 498, 883, 1324
TseFI GTSAC 3 cut(s) 874, 1319, 1379
TseI GCWGC 4 cut(s) 5, 108, 918, 1016
Tsp45I GTSAC 3 cut(s) 874, 1319, 1379
TspDTI ATGAA 9 cut(s) 125, 473, 504, 783, 951, 963, 995, 1086, 1237
TspRI CASTG 4 cut(s) 330, 498, 883, 1324
Van91I CCANNNNNTGG 1 cut(s) 1376
XagI CCTNNNNNAGG 1 cut(s) 608
XapI RAATTY 4 cut(s) 63, 1059, 1563, 1617
XceI RCATGY 4 cut(s) 402, 505, 842, 904
XhoI CTCGAG 1 cut(s) 508
XspI CTAG 6 cut(s) 68, 350, 630, 818, 864, 1633
ZrmI AGTACT 1 cut(s) 1139
Zsp2I ATGCAT 4 cut(s) 232, 670, 844, 1199
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.