MD03G1049700.v1.1

Late embryogenesis abundant protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0016133
Physical Location & Seq
Forward (+)
3970573 .. 3971307
735 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1049700.v1.1.491

Sequence Viewer

Length: 735 bp
ATGGTACATGGTCCATCCTTGCCTCCACCTCCTGGCCGCAACCAATCAAAGGCCAGCAGTGCGCGGCACGTTGCCATCTCTGAAGCTGCCTCAGCTCCCAGCAAGGTCCGCGATCCGGCCTATATGGTTCCTGACCTTGACCATGAGCGCCCCAAGCGTCGTGCAGGCTACGCTTGCTGCGCATGGTGCTGCTTGGTCTTCTTCGCCATTGTGATTCTCGCTCTAATTATTGCTTTCATTGCCATGGCCATCTTCCATTCGTACCTGCCAGAAATCTACATCCGAAGATTCAACGCCACCAGCCTCAACTTTACTAAAGACCACAATAAACTTGTCTTGAGGGGAAATGTACATTTTCTTGTGGAGTTCTTTAACAAGAATGATAAAACTGACCTCAAGTATGGTGCCTTCAAGATCAAGGTAGCGTCCGCGCATGTCCCGCTAGGTATCACCGGGTTTCCTGAGTTTGCTCAGGGCCAGAAGAACACGAAGTCGTTGAATGGAACCATTAAGGTTAATAAATCCGGGATTACCAAAGACGATGTAGATCAACTCAAAACCGACATAAACAATAGGGAGATGACACTGAACTTGGCCATGACCGGAAGCGTTAGCTTCCCCATTGCTGGAATTTTCTTTAACGGCATTCCCATCATTTCAAACTGCGATGCCAAGCAAAGGGAAGTTGATTTCGGAAACAAGGCTAAATGCGATTACAAGATTATCCCCATATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LEA_2

Protein Analysis

245

Amino Acids

26.99

Weight (kDa)

9.44

Isoelectric Point (pI)

33.32

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016133)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G30505
fragaria_vesca FvH4_3g40830
malus_domestica MD03G1049700.v1.1 MD11G1051900.v1.1
prunus_persica Prupe.6G039500_v2.0.a1
rosa_chinensis RchiOBHm_Chr5g0073561
rosa_laevigata RLG00000036395
rosa_multiflora Rmu_ssc0000226.1_g000002
rosa_roxburghii Rroxscaffold_1G00007720
rosa_rugosa Rorug05G0424800
rosa_samantha Rh5AG481900 Rh5BG502600 Rh5CG525700 Rh5DG514800
rosa_wichuraiana Rw5G044760

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 181
Acc36I ACCTGC 1 cut(s) 273
AccB1I GGYRCC 1 cut(s) 404
AccB7I CCANNNNNTGG 1 cut(s) 32
AccII CGCG 3 cut(s) 64, 111, 431
AciI CCGC 5 cut(s) 37, 64, 109, 429, 440
AclWI GGATC 1 cut(s) 107
AcoI YGGCCR 3 cut(s) 34, 246, 594
AcsI RAATTY 1 cut(s) 630
AcuI CTGAAG 1 cut(s) 102
AfaI GTAC 3 cut(s) 6, 263, 351
AfiI CCNNNNNNNGG 5 cut(s) 32, 49, 115, 626, 678
AgsI TTSAA 4 cut(s) 292, 412, 499, 660
AjnI CCWGG 1 cut(s) 31
AluBI AGCT 3 cut(s) 86, 95, 615
AluI AGCT 3 cut(s) 86, 95, 615
AlwI GGATC 1 cut(s) 107
AoxI GGCC 6 cut(s) 34, 51, 117, 246, 475, 594
ApeKI GCWGC 3 cut(s) 86, 177, 189
ApoI RAATTY 1 cut(s) 630
AspLEI GCGC 4 cut(s) 64, 150, 182, 433
AspS9I GGNCC 3 cut(s) 11, 106, 475
AsuC2I CCSGG 2 cut(s) 454, 526
AsuHPI GGTGA 1 cut(s) 442
AvaII GGWCC 2 cut(s) 11, 106
BalI TGGCCA 2 cut(s) 248, 596
BanI GGYRCC 1 cut(s) 404
BarI GAAGNNNNNNTAC 2 cut(s) 392, 424
BbsI GAAGAC 1 cut(s) 190
BbvCI CCTCAGC 1 cut(s) 91
BbvI GCAGC 3 cut(s) 73, 164, 176
BccI CCATC 4 cut(s) 22, 83, 257, 659
BceAI ACGGC 1 cut(s) 658
BciT130I CCWGG 1 cut(s) 33
BcnI CCSGG 2 cut(s) 454, 526
BfaI CTAG 1 cut(s) 443
BfoI RGCGCY 1 cut(s) 151
BfuAI ACCTGC 1 cut(s) 273
BisI GCNGC 5 cut(s) 37, 65, 87, 178, 190
BlsI GCNGC 5 cut(s) 38, 66, 88, 179, 191
Bme1390I CCNGG 3 cut(s) 33, 454, 526
Bme18I GGWCC 2 cut(s) 11, 106
BmgT120I GGNCC 3 cut(s) 11, 106, 475
BmiI GGNNCC 3 cut(s) 129, 406, 505
BmrFI CCNGG 3 cut(s) 33, 454, 526
BmsI GCATC 1 cut(s) 658
BpiI GAAGAC 1 cut(s) 190
Bpu10I CCTNAGC 2 cut(s) 91, 471
BpuEI CTTGAG 2 cut(s) 358, 380
BpuMI CCSGG 2 cut(s) 454, 526
BsaBI GATNNNNATC 1 cut(s) 546
BsaJI CCNNGG 1 cut(s) 243
BsaWI WCCGGW 1 cut(s) 602
Bsc4I CCNNNNNNNGG 5 cut(s) 32, 49, 115, 626, 678
Bse3DI GCAATG 2 cut(s) 237, 621
Bse8I GATNNNNATC 1 cut(s) 546
BseBI CCWGG 1 cut(s) 33
BseDI CCNNGG 1 cut(s) 243
BseGI GGATG 2 cut(s) 14, 279
BseJI GATNNNNATC 1 cut(s) 546
BseLI CCNNNNNNNGG 5 cut(s) 32, 49, 115, 626, 678
BseMI GCAATG 2 cut(s) 237, 621
BseMII CTCAG 3 cut(s) 105, 453, 485
BseXI GCAGC 3 cut(s) 73, 164, 176
BseYI CCCAGC 1 cut(s) 98
BsgI GTGCAG 1 cut(s) 183
Bsh1236I CGCG 3 cut(s) 64, 111, 431
BshFI GGCC 6 cut(s) 36, 53, 119, 248, 477, 596
BshNI GGYRCC 1 cut(s) 404
BsiSI CCGG 4 cut(s) 116, 453, 525, 603
BslFI GGGAC 1 cut(s) 422
BslI CCNNNNNNNGG 5 cut(s) 32, 49, 115, 626, 678
BsmFI GGGAC 1 cut(s) 422
BsmI GAATGC 1 cut(s) 645
BsnI GGCC 6 cut(s) 36, 53, 119, 248, 477, 596
Bsp1407I TGTACA 1 cut(s) 349
Bsp143I GATC 3 cut(s) 112, 414, 547
Bsp19I CCATGG 1 cut(s) 243
BspACI CCGC 5 cut(s) 37, 64, 109, 429, 440
BspANI GGCC 6 cut(s) 36, 53, 119, 248, 477, 596
BspCNI CTCAG 3 cut(s) 104, 454, 484
BspFNI CGCG 3 cut(s) 64, 111, 431
BspLI GGNNCC 3 cut(s) 129, 406, 505
BspMI ACCTGC 1 cut(s) 273
BspPI GGATC 1 cut(s) 107
BspT107I GGYRCC 1 cut(s) 404
BsrDI GCAATG 2 cut(s) 237, 621
BsrGI TGTACA 1 cut(s) 349
BssECI CCNNGG 1 cut(s) 243
BssMI GATC 3 cut(s) 112, 414, 547
BssT1I CCWWGG 1 cut(s) 243
Bst2UI CCWGG 1 cut(s) 33
BstAUI TGTACA 1 cut(s) 349
BstC8I GCNNGC 3 cut(s) 55, 166, 175
BstDEI CTNAG 3 cut(s) 91, 462, 471
BstDSI CCRYGG 1 cut(s) 243
BstF5I GGATG 2 cut(s) 14, 279
BstFNI CGCG 3 cut(s) 64, 111, 431
BstH2I RGCGCY 1 cut(s) 151
BstHHI GCGC 4 cut(s) 64, 150, 182, 433
BstKTI GATC 3 cut(s) 115, 417, 550
BstMBI GATC 3 cut(s) 112, 414, 547
BstNI CCWGG 1 cut(s) 33
BstNSI RCATGY 1 cut(s) 437
BstSCI CCNGG 3 cut(s) 31, 452, 524
BstUI CGCG 3 cut(s) 64, 111, 431
BstV1I GCAGC 3 cut(s) 73, 164, 176
BstV2I GAAGAC 1 cut(s) 190
BsuRI GGCC 6 cut(s) 36, 53, 119, 248, 477, 596
BtgI CCRYGG 1 cut(s) 243
BtgZI GCGATG 1 cut(s) 681
BtsCI GGATG 2 cut(s) 14, 279
BtsI GCAGTG 1 cut(s) 64
BtsIMutI CAGTG 2 cut(s) 64, 584
BveI ACCTGC 1 cut(s) 273
Cac8I GCNNGC 3 cut(s) 55, 166, 175
CfoI GCGC 4 cut(s) 64, 150, 182, 433
Cfr13I GGNCC 3 cut(s) 11, 106, 475
CseI GACGC 2 cut(s) 146, 414
Csp6I GTAC 3 cut(s) 5, 262, 350
CviAII CATG 6 cut(s) 8, 143, 183, 244, 434, 598
CviQI GTAC 3 cut(s) 5, 262, 350
DdeI CTNAG 3 cut(s) 91, 462, 471
DpnI GATC 3 cut(s) 114, 416, 549
DpnII GATC 3 cut(s) 112, 414, 547
EaeI YGGCCR 3 cut(s) 34, 246, 594
Eco130I CCWWGG 1 cut(s) 243
Eco47I GGWCC 2 cut(s) 11, 106
Eco57I CTGAAG 1 cut(s) 102
EcoRII CCWGG 1 cut(s) 31
EcoT14I CCWWGG 1 cut(s) 243
ErhI CCWWGG 1 cut(s) 243
FaeI CATG 6 cut(s) 11, 146, 186, 247, 437, 601
FaqI GGGAC 1 cut(s) 422
FatI CATG 6 cut(s) 7, 142, 182, 243, 433, 597
FauI CCCGC 1 cut(s) 447
FauNDI CATATG 1 cut(s) 731
Fnu4HI GCNGC 5 cut(s) 37, 65, 87, 178, 190
FokI GGATG 1 cut(s) 266
Fsp4HI GCNGC 5 cut(s) 37, 65, 87, 178, 190
FspBI CTAG 1 cut(s) 443
FspI TGCGCA 1 cut(s) 181
GlaI GCGC 4 cut(s) 63, 149, 181, 432
GluI GCNGC 5 cut(s) 37, 65, 87, 178, 190
GsaI CCCAGC 1 cut(s) 102
HaeII RGCGCY 1 cut(s) 151
HaeIII GGCC 6 cut(s) 36, 53, 119, 248, 477, 596
HapII CCGG 4 cut(s) 116, 453, 525, 603
HgaI GACGC 2 cut(s) 146, 414
HhaI GCGC 4 cut(s) 64, 150, 182, 433
Hin1II CATG 6 cut(s) 11, 146, 186, 247, 437, 601
Hin6I GCGC 4 cut(s) 62, 148, 180, 431
HinP1I GCGC 4 cut(s) 62, 148, 180, 431
HinfI GANTC 2 cut(s) 214, 288
HpaII CCGG 4 cut(s) 116, 453, 525, 603
HphI GGTGA 1 cut(s) 442
Hpy188I TCNGA 3 cut(s) 82, 284, 695
Hpy188III TCNNGA 4 cut(s) 131, 337, 412, 461
Hpy99I CGWCG 1 cut(s) 162
HpyAV CCTTC 1 cut(s) 418
HpyCH4IV ACGT 1 cut(s) 69
HpyCH4V TGCA 1 cut(s) 164
HpyF3I CTNAG 3 cut(s) 91, 462, 471
HpySE526I ACGT 1 cut(s) 69
Hsp92II CATG 6 cut(s) 11, 146, 186, 247, 437, 601
HspAI GCGC 4 cut(s) 62, 148, 180, 431
Kzo9I GATC 3 cut(s) 112, 414, 547
LmnI GCTCC 1 cut(s) 100
Lsp1109I GCAGC 3 cut(s) 73, 164, 176
LweI GCATC 1 cut(s) 658
MaeI CTAG 1 cut(s) 443
MaeII ACGT 1 cut(s) 69
MalI GATC 3 cut(s) 114, 416, 549
MboI GATC 3 cut(s) 112, 414, 547
MboII GAAGA 5 cut(s) 190, 193, 244, 297, 493
MlsI TGGCCA 2 cut(s) 248, 596
MluCI AATT 2 cut(s) 225, 630
MluNI TGGCCA 2 cut(s) 248, 596
MnlI CCTC 6 cut(s) 33, 39, 100, 314, 333, 404
Mox20I TGGCCA 2 cut(s) 248, 596
MscI TGGCCA 2 cut(s) 248, 596
MseI TTAA 4 cut(s) 372, 510, 516, 639
MslI CAYNNNNRTG 1 cut(s) 242
Msp20I TGGCCA 2 cut(s) 248, 596
MspI CCGG 4 cut(s) 116, 453, 525, 603
MspR9I CCNGG 3 cut(s) 33, 454, 526
Mva1269I GAATGC 1 cut(s) 645
MvaI CCWGG 1 cut(s) 33
MvnI CGCG 3 cut(s) 64, 111, 431
NciI CCSGG 2 cut(s) 454, 526
NcoI CCATGG 1 cut(s) 243
NdeI CATATG 1 cut(s) 731
NdeII GATC 3 cut(s) 112, 414, 547
NlaIII CATG 6 cut(s) 11, 146, 186, 247, 437, 601
NlaIV GGNNCC 3 cut(s) 129, 406, 505
NsbI TGCGCA 1 cut(s) 181
NspI RCATGY 1 cut(s) 437
PctI GAATGC 1 cut(s) 645
PfeI GAWTC 2 cut(s) 214, 288
PflMI CCANNNNNTGG 1 cut(s) 32
PfoI TCCNGGA 1 cut(s) 524
PkrI GCNGC 5 cut(s) 38, 66, 88, 179, 191
Psp6I CCWGG 1 cut(s) 31
PspFI CCCAGC 1 cut(s) 98
PspGI CCWGG 1 cut(s) 31
PspN4I GGNNCC 3 cut(s) 129, 406, 505
PspPI GGNCC 3 cut(s) 11, 106, 475
RsaI GTAC 3 cut(s) 6, 263, 351
RsaNI GTAC 3 cut(s) 5, 262, 350
RseI CAYNNNNRTG 1 cut(s) 242
SaqAI TTAA 4 cut(s) 372, 510, 516, 639
SatI GCNGC 5 cut(s) 37, 65, 87, 178, 190
Sau3AI GATC 3 cut(s) 112, 414, 547
Sau96I GGNCC 3 cut(s) 11, 106, 475
ScrFI CCNGG 3 cut(s) 33, 454, 526
SfaNI GCATC 1 cut(s) 658
SinI GGWCC 2 cut(s) 11, 106
SmiMI CAYNNNNRTG 1 cut(s) 242
SmlI CTYRAG 2 cut(s) 337, 395
SmoI CTYRAG 2 cut(s) 337, 395
Sse9I AATT 2 cut(s) 225, 630
SsiI CCGC 5 cut(s) 37, 64, 109, 429, 440
SspMI CTAG 1 cut(s) 443
StyD4I CCNGG 3 cut(s) 31, 452, 524
StyI CCWWGG 1 cut(s) 243
TaiI ACGT 1 cut(s) 72
TasI AATT 2 cut(s) 225, 630
TatI WGTACW 1 cut(s) 349
TauI GCSGC 2 cut(s) 39, 67
TfiI GAWTC 2 cut(s) 214, 288
Tru1I TTAA 4 cut(s) 372, 510, 516, 639
Tru9I TTAA 4 cut(s) 372, 510, 516, 639
TscAI CASTG 2 cut(s) 64, 591
TseI GCWGC 3 cut(s) 86, 177, 189
TspDTI ATGAA 1 cut(s) 226
TspRI CASTG 2 cut(s) 64, 591
Van91I CCANNNNNTGG 1 cut(s) 32
VpaK11BI GGWCC 2 cut(s) 11, 106
XapI RAATTY 1 cut(s) 630
XceI RCATGY 1 cut(s) 437
XspI CTAG 1 cut(s) 443
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.