MD03G1058700.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0012600
Physical Location & Seq
Forward (+)
4724982 .. 4726341
1360 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1058700.v1.1.491

Sequence Viewer

Length: 486 bp
ATGGTCTCCAAGGTGCAGATGAAGAGAGCGCCAGTATCCAAGAAACTGCTAACCCTTCGTTCCATCTCTAAGTCTCATGCGCACAGTAAAACCGCAATTGTATTCGATGCATCAAAATATATACAAAACTTAAAGCGCAAAGTGGAGGAAATGAATCTACAGACGATTGCTTCAGCGCAAACCTCAACTTCCCATAACCCCTTTTCTGTGCAACTGAAAGTCGAAGCTCGTGAGGAAGGCTTTCTGATTAAGATGTTCAGTGAAAAAAGCTGCAGCGGGTTACTTGTTTTCGTATTGGAAGCTTTTGAAGAGCTAGGCCTTGATGTGCATCAAGCTAGGGTTTCTTGTTCCAACAATTTTCTTCTAGAAGCTGTTGGAACAATCAATGATAATCAAAGTGCTGAACAGAAAGATGTTGAGGTAGTGAAAGAAGCGATGTTGCAGGCCATTCAAAACTGGAGTGAAGTTTCCCAACAACAAGAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

162

Amino Acids

18.04

Weight (kDa)

6.91

Isoelectric Point (pI)

42.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
bHLH-TF_ACT-like_plant PF22754 71 - 151 2.8e-22 Plant bHLH transcription factor, ACT-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 81
AciI CCGC 2 cut(s) 93, 276
AcuI CTGAAG 1 cut(s) 156
AgsI TTSAA 2 cut(s) 308, 452
AluBI AGCT 6 cut(s) 227, 270, 302, 313, 335, 371
AluI AGCT 6 cut(s) 227, 270, 302, 313, 335, 371
Alw26I GTCTC 2 cut(s) 10, 78
AoxI GGCC 2 cut(s) 316, 444
ApeKI GCWGC 2 cut(s) 270, 273
Asp700I GAANNNNTTC 1 cut(s) 240
AspLEI GCGC 4 cut(s) 31, 82, 138, 178
BauI CACGAG 1 cut(s) 228
BbvI GCAGC 2 cut(s) 257, 285
BccI CCATC 1 cut(s) 71
BciVI GTATCC 1 cut(s) 46
BcoDI GTCTC 2 cut(s) 10, 78
BfaI CTAG 3 cut(s) 314, 336, 365
BfmI CTRYAG 2 cut(s) 158, 271
BfoI RGCGCY 1 cut(s) 32
BfuI GTATCC 1 cut(s) 46
BisI GCNGC 2 cut(s) 271, 274
BlsI GCNGC 2 cut(s) 272, 275
BmsI GCATC 3 cut(s) 97, 119, 337
BpmI CTGGAG 1 cut(s) 478
BsaBI GATNNNNATC 1 cut(s) 327
BsaI GGTCTC 1 cut(s) 10
BsaJI CCNNGG 1 cut(s) 9
Bse1I ACTGG 2 cut(s) 32, 461
Bse8I GATNNNNATC 1 cut(s) 327
BseDI CCNNGG 1 cut(s) 9
BseJI GATNNNNATC 1 cut(s) 327
BseNI ACTGG 2 cut(s) 32, 461
BseXI GCAGC 2 cut(s) 257, 285
BsgI GTGCAG 1 cut(s) 35
BshFI GGCC 2 cut(s) 318, 446
BsmAI GTCTC 2 cut(s) 10, 78
BsnI GGCC 2 cut(s) 318, 446
Bso31I GGTCTC 1 cut(s) 10
BspACI CCGC 2 cut(s) 93, 276
BspANI GGCC 2 cut(s) 318, 446
BspMAI CTGCAG 1 cut(s) 275
BspQI GCTCTTC 1 cut(s) 303
BspTNI GGTCTC 1 cut(s) 10
BsrI ACTGG 2 cut(s) 32, 461
BssECI CCNNGG 1 cut(s) 9
BssSI CACGAG 1 cut(s) 228
BssT1I CCWWGG 1 cut(s) 9
Bst2BI CACGAG 1 cut(s) 228
Bst4CI ACNGT 1 cut(s) 86
Bst6I CTCTTC 2 cut(s) 17, 303
BstC8I GCNNGC 1 cut(s) 444
BstDEI CTNAG 1 cut(s) 69
BstH2I RGCGCY 1 cut(s) 32
BstHHI GCGC 4 cut(s) 31, 82, 138, 178
BstMAI GTCTC 2 cut(s) 10, 78
BstSFI CTRYAG 2 cut(s) 158, 271
BstV1I GCAGC 2 cut(s) 257, 285
BsuI GTATCC 1 cut(s) 46
BsuRI GGCC 2 cut(s) 318, 446
BtgZI GCGATG 1 cut(s) 449
BtsIMutI CAGTG 1 cut(s) 265
Cac8I GCNNGC 1 cut(s) 444
CfoI GCGC 4 cut(s) 31, 82, 138, 178
CviAII CATG 1 cut(s) 77
CviJI RGCY 9 cut(s) 227, 240, 270, 302, 313, 318, 335, 371, 446
CviKI_1 RGCY 9 cut(s) 227, 240, 270, 302, 313, 318, 335, 371, 446
DdeI CTNAG 1 cut(s) 69
Eam1104I CTCTTC 2 cut(s) 17, 303
EarI CTCTTC 2 cut(s) 17, 303
Eco130I CCWWGG 1 cut(s) 9
Eco147I AGGCCT 1 cut(s) 318
Eco31I GGTCTC 1 cut(s) 10
Eco57I CTGAAG 1 cut(s) 156
EcoT14I CCWWGG 1 cut(s) 9
EcoT22I ATGCAT 1 cut(s) 112
ErhI CCWWGG 1 cut(s) 9
FaeI CATG 1 cut(s) 80
FaiI YATR 4 cut(s) 78, 120, 122, 195
FatI CATG 1 cut(s) 76
FauI CCCGC 1 cut(s) 269
Fnu4HI GCNGC 2 cut(s) 271, 274
Fsp4HI GCNGC 2 cut(s) 271, 274
FspAI RTGCGCAY 1 cut(s) 81
FspBI CTAG 3 cut(s) 314, 336, 365
FspI TGCGCA 1 cut(s) 81
GlaI GCGC 4 cut(s) 30, 81, 137, 177
GluI GCNGC 2 cut(s) 271, 274
GsuI CTGGAG 1 cut(s) 478
HaeII RGCGCY 1 cut(s) 32
HaeIII GGCC 2 cut(s) 318, 446
HhaI GCGC 4 cut(s) 31, 82, 138, 178
Hin1II CATG 1 cut(s) 80
Hin6I GCGC 4 cut(s) 29, 80, 136, 176
HinP1I GCGC 4 cut(s) 29, 80, 136, 176
HindIII AAGCTT 1 cut(s) 300
HinfI GANTC 1 cut(s) 154
Hpy188I TCNGA 1 cut(s) 246
Hpy188III TCNNGA 2 cut(s) 230, 365
HpyAV CCTTC 2 cut(s) 65, 230
HpyCH4III ACNGT 1 cut(s) 86
HpyCH4V TGCA 6 cut(s) 16, 110, 211, 273, 328, 442
HpyF3I CTNAG 1 cut(s) 69
Hsp92II CATG 1 cut(s) 80
HspAI GCGC 4 cut(s) 29, 80, 136, 176
LguI GCTCTTC 1 cut(s) 303
LpnPI CCDG 3 cut(s) 45, 428, 442
Lsp1109I GCAGC 2 cut(s) 257, 285
LweI GCATC 3 cut(s) 97, 119, 337
MaeI CTAG 3 cut(s) 314, 336, 365
MaeIII GTNAC 1 cut(s) 279
MboII GAAGA 3 cut(s) 34, 320, 353
MfeI CAATTG 1 cut(s) 96
MluCI AATT 2 cut(s) 96, 355
MmeI TCCRAC 2 cut(s) 355, 375
MnlI CCTC 4 cut(s) 139, 193, 226, 412
Mph1103I ATGCAT 1 cut(s) 112
MroXI GAANNNNTTC 1 cut(s) 240
MseI TTAA 2 cut(s) 131, 249
MspA1I CMGCKG 1 cut(s) 276
MunI CAATTG 1 cut(s) 96
NlaIII CATG 1 cut(s) 80
NsbI TGCGCA 1 cut(s) 81
NsiI ATGCAT 1 cut(s) 112
PceI AGGCCT 1 cut(s) 318
PciSI GCTCTTC 1 cut(s) 303
PdmI GAANNNNTTC 1 cut(s) 240
PfeI GAWTC 1 cut(s) 154
PkrI GCNGC 2 cut(s) 272, 275
PstI CTGCAG 1 cut(s) 275
SapI GCTCTTC 1 cut(s) 303
SaqAI TTAA 2 cut(s) 131, 249
SatI GCNGC 2 cut(s) 271, 274
SetI ASST 9 cut(s) 15, 185, 229, 272, 304, 315, 337, 373, 423
SfaNI GCATC 3 cut(s) 97, 119, 337
SfcI CTRYAG 2 cut(s) 158, 271
Sse9I AATT 2 cut(s) 96, 355
SseBI AGGCCT 1 cut(s) 318
SsiI CCGC 2 cut(s) 93, 276
SspMI CTAG 3 cut(s) 314, 336, 365
StuI AGGCCT 1 cut(s) 318
StyI CCWWGG 1 cut(s) 9
TaaI ACNGT 1 cut(s) 86
TaqI TCGA 2 cut(s) 105, 222
TasI AATT 2 cut(s) 96, 355
TfiI GAWTC 1 cut(s) 154
Tru1I TTAA 2 cut(s) 131, 249
Tru9I TTAA 2 cut(s) 131, 249
TscAI CASTG 1 cut(s) 265
TseI GCWGC 2 cut(s) 270, 273
TspDTI ATGAA 2 cut(s) 35, 167
TspRI CASTG 1 cut(s) 265
XbaI TCTAGA 1 cut(s) 364
XmnI GAANNNNTTC 1 cut(s) 240
XspI CTAG 3 cut(s) 314, 336, 365
Zsp2I ATGCAT 1 cut(s) 112
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.