Rh5AG472900

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0012600
Physical Location & Seq
Forward (+)
81246763 .. 81248648
1886 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG472900.1

Sequence Viewer

Length: 489 bp
ATGCATCAGCAAGAAAGAAGAGAGAGTCAGAGGGAGAGAGAGAGAGAGATCGAAGCCATGGTATCCAAACCGGTGCAGAAGAGAGATCCAGTATACAAGAAACTTCAATCCCTTCGTTCAATCTCCAACTCCCCTGCGCACAGTAAAAGCTCAATTATATTGGATGCAGTAACATATATACAAGAACTGAAGCGAAAAGTGGAGGAAATGAATCAAGAGATCGTTGCTGCCAGAGCTCAACTCTCAACACCCGCCCAAAACCCGTTCCCTATGCAACTGAAAGTTGAACCTCGAGAGGAGGATCAAGCAGGTTTTCAAATAAAGATGTTCACTGAAAAGAGTTGCAGTGGGTTGCTTGTTTTCGTATTGGAAGCGTTTGAAGAGTTAGGCCTAGATGTTCATCAAGCTAGGGTTTCTTGCTCCAACAATTTTCTTTTAGAAGCAGTGGCCACAATTAACAATGTTAGCATATATAAAACCGAGTCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

162

Amino Acids

18.55

Weight (kDa)

6.34

Isoelectric Point (pI)

63.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
bHLH-TF_ACT-like_plant PF22754 94 - 149 6e-15 Plant bHLH transcription factor, ACT-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 138
Acc36I ACCTGC 1 cut(s) 299
AccI GTMKAC 1 cut(s) 93
AciI CCGC 1 cut(s) 252
AclWI GGATC 2 cut(s) 80, 309
AcoI YGGCCR 1 cut(s) 447
AcuI CTGAAG 1 cut(s) 209
AgeI ACCGGT 1 cut(s) 70
AgsI TTSAA 5 cut(s) 107, 120, 287, 317, 380
AluBI AGCT 3 cut(s) 150, 236, 407
AluI AGCT 3 cut(s) 150, 236, 407
Alw21I GWGCWC 1 cut(s) 238
AlwI GGATC 2 cut(s) 80, 309
Ama87I CYCGRG 1 cut(s) 291
AoxI GGCC 2 cut(s) 388, 447
ApeKI GCWGC 1 cut(s) 227
AsiGI ACCGGT 1 cut(s) 70
AspLEI GCGC 1 cut(s) 139
AvaI CYCGRG 1 cut(s) 291
BalI TGGCCA 1 cut(s) 449
BanII GRGCYC 1 cut(s) 238
Bbv12I GWGCWC 1 cut(s) 238
BbvI GCAGC 1 cut(s) 214
BciVI GTATCC 1 cut(s) 73
BfaI CTAG 2 cut(s) 392, 408
BfuAI ACCTGC 1 cut(s) 299
BfuI GTATCC 1 cut(s) 73
BisI GCNGC 1 cut(s) 228
BlsI GCNGC 1 cut(s) 229
BmeT110I CYCGRG 1 cut(s) 291
BmsI GCATC 2 cut(s) 13, 154
BplI GAGNNNNNCTC 2 cut(s) 225, 257
BsaBI GATNNNNATC 1 cut(s) 399
BsaJI CCNNGG 1 cut(s) 57
BsaWI WCCGGW 1 cut(s) 70
Bse118I RCCGGY 1 cut(s) 70
Bse1I ACTGG 1 cut(s) 89
Bse8I GATNNNNATC 1 cut(s) 399
BseDI CCNNGG 1 cut(s) 57
BseGI GGATG 1 cut(s) 169
BseJI GATNNNNATC 1 cut(s) 399
BseNI ACTGG 1 cut(s) 89
BseRI GAGGAG 1 cut(s) 311
BseXI GCAGC 1 cut(s) 214
BsgI GTGCAG 1 cut(s) 95
BshFI GGCC 2 cut(s) 390, 449
BshTI ACCGGT 1 cut(s) 70
BsiHKAI GWGCWC 1 cut(s) 238
BsiHKCI CYCGRG 1 cut(s) 291
BsiSI CCGG 1 cut(s) 71
BsnI GGCC 2 cut(s) 390, 449
BsoBI CYCGRG 1 cut(s) 291
Bsp1286I GDGCHC 1 cut(s) 238
Bsp143I GATC 4 cut(s) 48, 85, 219, 301
Bsp19I CCATGG 1 cut(s) 57
BspACI CCGC 1 cut(s) 252
BspANI GGCC 2 cut(s) 390, 449
BspMI ACCTGC 1 cut(s) 299
BspPI GGATC 2 cut(s) 80, 309
BsrFI RCCGGY 1 cut(s) 70
BsrI ACTGG 1 cut(s) 89
BssAI RCCGGY 1 cut(s) 70
BssECI CCNNGG 1 cut(s) 57
BssMI GATC 4 cut(s) 48, 85, 219, 301
BssNAI GTATAC 1 cut(s) 94
BssT1I CCWWGG 1 cut(s) 57
Bst1107I GTATAC 1 cut(s) 94
Bst4CI ACNGT 1 cut(s) 143
Bst6I CTCTTC 3 cut(s) 13, 74, 375
BstDEI CTNAG 1 cut(s) 486
BstDSI CCRYGG 1 cut(s) 57
BstF5I GGATG 1 cut(s) 169
BstHHI GCGC 1 cut(s) 139
BstKTI GATC 4 cut(s) 51, 88, 222, 304
BstMBI GATC 4 cut(s) 48, 85, 219, 301
BstMWI GCNNNNNNNGC 1 cut(s) 233
BstV1I GCAGC 1 cut(s) 214
BstX2I RGATCY 1 cut(s) 85
BstYI RGATCY 1 cut(s) 85
BstZ17I GTATAC 1 cut(s) 94
BsuI GTATCC 1 cut(s) 73
BsuRI GGCC 2 cut(s) 390, 449
BtgI CCRYGG 1 cut(s) 57
BtsCI GGATG 1 cut(s) 169
BtsI GCAGTG 2 cut(s) 352, 450
BtsIMutI CAGTG 3 cut(s) 330, 352, 450
BveI ACCTGC 1 cut(s) 299
CfoI GCGC 1 cut(s) 139
Cfr10I RCCGGY 1 cut(s) 70
CspAI ACCGGT 1 cut(s) 70
CviAII CATG 1 cut(s) 58
CviJI RGCY 6 cut(s) 56, 150, 236, 390, 407, 449
CviKI_1 RGCY 6 cut(s) 56, 150, 236, 390, 407, 449
DdeI CTNAG 1 cut(s) 486
DpnI GATC 4 cut(s) 50, 87, 221, 303
DpnII GATC 4 cut(s) 48, 85, 219, 301
EaeI YGGCCR 1 cut(s) 447
Eam1104I CTCTTC 3 cut(s) 13, 74, 375
EarI CTCTTC 3 cut(s) 13, 74, 375
Ecl136II GAGCTC 1 cut(s) 236
Eco130I CCWWGG 1 cut(s) 57
Eco147I AGGCCT 1 cut(s) 390
Eco24I GRGCYC 1 cut(s) 238
Eco53kI GAGCTC 1 cut(s) 236
Eco57I CTGAAG 1 cut(s) 209
Eco88I CYCGRG 1 cut(s) 291
EcoICRI GAGCTC 1 cut(s) 236
EcoT14I CCWWGG 1 cut(s) 57
EcoT22I ATGCAT 1 cut(s) 6
EcoT38I GRGCYC 1 cut(s) 238
ErhI CCWWGG 1 cut(s) 57
FaeI CATG 1 cut(s) 61
FatI CATG 1 cut(s) 57
FauI CCCGC 1 cut(s) 259
FblI GTMKAC 1 cut(s) 93
Fnu4HI GCNGC 1 cut(s) 228
FokI GGATG 1 cut(s) 176
FriOI GRGCYC 1 cut(s) 238
Fsp4HI GCNGC 1 cut(s) 228
FspBI CTAG 2 cut(s) 392, 408
FspI TGCGCA 1 cut(s) 138
GlaI GCGC 1 cut(s) 138
GluI GCNGC 1 cut(s) 228
HaeIII GGCC 2 cut(s) 390, 449
HapII CCGG 1 cut(s) 71
HhaI GCGC 1 cut(s) 139
Hin1II CATG 1 cut(s) 61
Hin6I GCGC 1 cut(s) 137
HinP1I GCGC 1 cut(s) 137
HinfI GANTC 3 cut(s) 25, 211, 482
HpaII CCGG 1 cut(s) 71
Hpy166II GTNNAC 2 cut(s) 94, 330
Hpy188I TCNGA 1 cut(s) 30
Hpy188III TCNNGA 2 cut(s) 215, 293
Hpy8I GTNNAC 2 cut(s) 94, 330
HpyAV CCTTC 1 cut(s) 122
HpyCH4III ACNGT 1 cut(s) 143
HpyCH4V TGCA 5 cut(s) 4, 76, 167, 274, 345
HpyF10VI GCNNNNNNNGC 1 cut(s) 233
HpyF3I CTNAG 1 cut(s) 486
Hsp92II CATG 1 cut(s) 61
HspAI GCGC 1 cut(s) 137
Kzo9I GATC 4 cut(s) 48, 85, 219, 301
LmnI GCTCC 1 cut(s) 425
LpnPI CCDG 5 cut(s) 84, 102, 147, 244, 294
Lsp1109I GCAGC 1 cut(s) 214
LweI GCATC 2 cut(s) 13, 154
MaeI CTAG 2 cut(s) 392, 408
MaeIII GTNAC 1 cut(s) 169
MalI GATC 4 cut(s) 50, 87, 221, 303
MboI GATC 4 cut(s) 48, 85, 219, 301
MboII GAAGA 3 cut(s) 30, 91, 392
MflI RGATCY 1 cut(s) 85
MhlI GDGCHC 1 cut(s) 238
MlsI TGGCCA 1 cut(s) 449
MluCI AATT 3 cut(s) 153, 427, 453
MluNI TGGCCA 1 cut(s) 449
MlyI GAGTC 1 cut(s) 34
MmeI TCCRAC 2 cut(s) 150, 447
MnlI CCTC 5 cut(s) 24, 196, 289, 292, 300
Mox20I TGGCCA 1 cut(s) 449
Mph1103I ATGCAT 1 cut(s) 6
MscI TGGCCA 1 cut(s) 449
MseI TTAA 1 cut(s) 456
Msp20I TGGCCA 1 cut(s) 449
MspI CCGG 1 cut(s) 71
MwoI GCNNNNNNNGC 1 cut(s) 233
NcoI CCATGG 1 cut(s) 57
NdeII GATC 4 cut(s) 48, 85, 219, 301
NlaIII CATG 1 cut(s) 61
NsbI TGCGCA 1 cut(s) 138
NsiI ATGCAT 1 cut(s) 6
PaeR7I CTCGAG 1 cut(s) 291
PceI AGGCCT 1 cut(s) 390
PfeI GAWTC 1 cut(s) 211
PinAI ACCGGT 1 cut(s) 70
PkrI GCNGC 1 cut(s) 229
PleI GAGTC 1 cut(s) 33
PpsI GAGTC 1 cut(s) 33
Psp124BI GAGCTC 1 cut(s) 238
PsuI RGATCY 1 cut(s) 85
SacI GAGCTC 1 cut(s) 238
SaqAI TTAA 1 cut(s) 456
SatI GCNGC 1 cut(s) 228
Sau3AI GATC 4 cut(s) 48, 85, 219, 301
SchI GAGTC 1 cut(s) 34
SduI GDGCHC 1 cut(s) 238
SetI ASST 5 cut(s) 152, 238, 292, 313, 409
SfaNI GCATC 2 cut(s) 13, 154
Sfr274I CTCGAG 1 cut(s) 291
SlaI CTCGAG 1 cut(s) 291
SmlI CTYRAG 1 cut(s) 291
SmoI CTYRAG 1 cut(s) 291
Sse9I AATT 3 cut(s) 153, 427, 453
SseBI AGGCCT 1 cut(s) 390
SsiI CCGC 1 cut(s) 252
SspMI CTAG 2 cut(s) 392, 408
SstI GAGCTC 1 cut(s) 238
StuI AGGCCT 1 cut(s) 390
StyI CCWWGG 1 cut(s) 57
TaaI ACNGT 1 cut(s) 143
TaqI TCGA 2 cut(s) 51, 292
TasI AATT 3 cut(s) 153, 427, 453
TfiI GAWTC 1 cut(s) 211
Tru1I TTAA 1 cut(s) 456
Tru9I TTAA 1 cut(s) 456
TscAI CASTG 3 cut(s) 337, 352, 450
TseI GCWGC 1 cut(s) 227
TspDTI ATGAA 2 cut(s) 224, 389
TspRI CASTG 3 cut(s) 337, 352, 450
XhoI CTCGAG 1 cut(s) 291
XmiI GTMKAC 1 cut(s) 93
XspI CTAG 2 cut(s) 392, 408
Zsp2I ATGCAT 1 cut(s) 6
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.