Rh5CG516800

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5C
OG0012600
Physical Location & Seq
Forward (+)
72225991 .. 72227731
1741 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5CG516800.1

Sequence Viewer

Length: 351 bp
ATGAATCAAGAGATCGTTGCTGCCAGAGCTCAAGTCTCAACACCCGCCCAAAACCCGTTCCCTATGCAACTGAAAGTTGAACCTCGAGAGGAGGATCAAGCAGGTTTTCAAATAAAGATGTTCACTGAAAAGAGTTGCAGTGGGTTGCTTGTTTTCGTATTGGAAGCGTTTGAAGAGTTAGGCCTAGATGTTCATCAAGCTAGGGTTTCTTGCTCCAACAATTTTCTTTTAGAAGCAGTGGCCACAATTAACAATGACGACCAAAATGGTGATGATCATAACAAAGATGCCGAGGTAGTAAGAAAAGCGATGCTGCAAGCCATTCAAAGCTGGAGTGAAGTTTCCCAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

116

Amino Acids

12.94

Weight (kDa)

4.51

Isoelectric Point (pI)

42.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
bHLH-TF_ACT-like_plant PF22754 25 - 109 4.9e-19 Plant bHLH transcription factor, ACT-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 92
AciI CCGC 1 cut(s) 45
AclWI GGATC 1 cut(s) 102
AcoI YGGCCR 1 cut(s) 240
AgsI TTSAA 4 cut(s) 80, 110, 173, 326
AluBI AGCT 3 cut(s) 29, 200, 330
AluI AGCT 3 cut(s) 29, 200, 330
Alw21I GWGCWC 1 cut(s) 31
Alw26I GTCTC 1 cut(s) 40
AlwI GGATC 1 cut(s) 102
Ama87I CYCGRG 1 cut(s) 84
AoxI GGCC 2 cut(s) 181, 240
ApeKI GCWGC 2 cut(s) 20, 313
AsuHPI GGTGA 1 cut(s) 281
AvaI CYCGRG 1 cut(s) 84
BalI TGGCCA 1 cut(s) 242
BanII GRGCYC 1 cut(s) 31
Bbv12I GWGCWC 1 cut(s) 31
BbvI GCAGC 2 cut(s) 7, 300
BclI TGATCA 1 cut(s) 274
BcoDI GTCTC 1 cut(s) 40
BfaI CTAG 2 cut(s) 185, 201
BfuAI ACCTGC 1 cut(s) 92
BisI GCNGC 2 cut(s) 21, 314
BlsI GCNGC 2 cut(s) 22, 315
BmeT110I CYCGRG 1 cut(s) 84
BmsI GCATC 2 cut(s) 277, 300
BpuEI CTTGAG 1 cut(s) 15
BsaBI GATNNNNATC 1 cut(s) 192
BsaJI CCNNGG 1 cut(s) 291
Bse8I GATNNNNATC 1 cut(s) 192
BseDI CCNNGG 1 cut(s) 291
BseJI GATNNNNATC 1 cut(s) 192
BseRI GAGGAG 1 cut(s) 104
BseXI GCAGC 2 cut(s) 7, 300
BshFI GGCC 2 cut(s) 183, 242
BsiHKAI GWGCWC 1 cut(s) 31
BsiHKCI CYCGRG 1 cut(s) 84
BsmAI GTCTC 1 cut(s) 40
BsnI GGCC 2 cut(s) 183, 242
BsoBI CYCGRG 1 cut(s) 84
Bsp1286I GDGCHC 1 cut(s) 31
Bsp143I GATC 3 cut(s) 12, 94, 274
BspACI CCGC 1 cut(s) 45
BspANI GGCC 2 cut(s) 183, 242
BspMI ACCTGC 1 cut(s) 92
BspPI GGATC 1 cut(s) 102
BssECI CCNNGG 1 cut(s) 291
BssMI GATC 3 cut(s) 12, 94, 274
Bst6I CTCTTC 1 cut(s) 168
BstC8I GCNNGC 1 cut(s) 318
BstKTI GATC 3 cut(s) 15, 97, 277
BstMAI GTCTC 1 cut(s) 40
BstMBI GATC 3 cut(s) 12, 94, 274
BstMWI GCNNNNNNNGC 1 cut(s) 26
BstV1I GCAGC 2 cut(s) 7, 300
BsuRI GGCC 2 cut(s) 183, 242
BtgZI GCGATG 1 cut(s) 323
BtsI GCAGTG 2 cut(s) 145, 243
BtsIMutI CAGTG 3 cut(s) 123, 145, 243
BveI ACCTGC 1 cut(s) 92
Cac8I GCNNGC 1 cut(s) 318
CviJI RGCY 6 cut(s) 29, 183, 200, 242, 320, 330
CviKI_1 RGCY 6 cut(s) 29, 183, 200, 242, 320, 330
DpnI GATC 3 cut(s) 14, 96, 276
DpnII GATC 3 cut(s) 12, 94, 274
EaeI YGGCCR 1 cut(s) 240
Eam1104I CTCTTC 1 cut(s) 168
EarI CTCTTC 1 cut(s) 168
Ecl136II GAGCTC 1 cut(s) 29
Eco147I AGGCCT 1 cut(s) 183
Eco24I GRGCYC 1 cut(s) 31
Eco53kI GAGCTC 1 cut(s) 29
Eco88I CYCGRG 1 cut(s) 84
EcoICRI GAGCTC 1 cut(s) 29
EcoT38I GRGCYC 1 cut(s) 31
FaiI YATR 2 cut(s) 65, 279
FauI CCCGC 1 cut(s) 52
FbaI TGATCA 1 cut(s) 274
Fnu4HI GCNGC 2 cut(s) 21, 314
FriOI GRGCYC 1 cut(s) 31
Fsp4HI GCNGC 2 cut(s) 21, 314
FspBI CTAG 2 cut(s) 185, 201
GluI GCNGC 2 cut(s) 21, 314
HaeIII GGCC 2 cut(s) 183, 242
HinfI GANTC 1 cut(s) 4
HphI GGTGA 1 cut(s) 281
Hpy166II GTNNAC 1 cut(s) 123
Hpy188III TCNNGA 2 cut(s) 8, 86
Hpy8I GTNNAC 1 cut(s) 123
HpyCH4V TGCA 3 cut(s) 67, 138, 316
HpyF10VI GCNNNNNNNGC 1 cut(s) 26
Ksp22I TGATCA 1 cut(s) 274
Kzo9I GATC 3 cut(s) 12, 94, 274
LmnI GCTCC 1 cut(s) 218
LpnPI CCDG 3 cut(s) 37, 87, 316
Lsp1109I GCAGC 2 cut(s) 7, 300
LweI GCATC 2 cut(s) 277, 300
MaeI CTAG 2 cut(s) 185, 201
MalI GATC 3 cut(s) 14, 96, 276
MboI GATC 3 cut(s) 12, 94, 274
MboII GAAGA 1 cut(s) 185
MhlI GDGCHC 1 cut(s) 31
MlsI TGGCCA 1 cut(s) 242
MluCI AATT 2 cut(s) 220, 246
MluNI TGGCCA 1 cut(s) 242
MmeI TCCRAC 1 cut(s) 240
MnlI CCTC 4 cut(s) 82, 85, 93, 286
Mox20I TGGCCA 1 cut(s) 242
MscI TGGCCA 1 cut(s) 242
MseI TTAA 1 cut(s) 249
Msp20I TGGCCA 1 cut(s) 242
MwoI GCNNNNNNNGC 1 cut(s) 26
NdeII GATC 3 cut(s) 12, 94, 274
NmeAIII GCCGAG 1 cut(s) 316
PaeR7I CTCGAG 1 cut(s) 84
PceI AGGCCT 1 cut(s) 183
PfeI GAWTC 1 cut(s) 4
PkrI GCNGC 2 cut(s) 22, 315
Psp124BI GAGCTC 1 cut(s) 31
SacI GAGCTC 1 cut(s) 31
SaqAI TTAA 1 cut(s) 249
SatI GCNGC 2 cut(s) 21, 314
Sau3AI GATC 3 cut(s) 12, 94, 274
SduI GDGCHC 1 cut(s) 31
SetI ASST 6 cut(s) 31, 85, 106, 202, 297, 332
SfaNI GCATC 2 cut(s) 277, 300
Sfr274I CTCGAG 1 cut(s) 84
SlaI CTCGAG 1 cut(s) 84
SmlI CTYRAG 2 cut(s) 30, 84
SmoI CTYRAG 2 cut(s) 30, 84
Sse9I AATT 2 cut(s) 220, 246
SseBI AGGCCT 1 cut(s) 183
SsiI CCGC 1 cut(s) 45
SspMI CTAG 2 cut(s) 185, 201
SstI GAGCTC 1 cut(s) 31
StuI AGGCCT 1 cut(s) 183
TaqI TCGA 1 cut(s) 85
TasI AATT 2 cut(s) 220, 246
TfiI GAWTC 1 cut(s) 4
Tru1I TTAA 1 cut(s) 249
Tru9I TTAA 1 cut(s) 249
TscAI CASTG 3 cut(s) 130, 145, 243
TseI GCWGC 2 cut(s) 20, 313
TspDTI ATGAA 2 cut(s) 17, 182
TspRI CASTG 3 cut(s) 130, 145, 243
XhoI CTCGAG 1 cut(s) 84
XspI CTAG 2 cut(s) 185, 201
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.