MD11G1060700.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0012600
Physical Location & Seq
Forward (+)
5291041 .. 5292687
1647 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1060700.v1.1.491

Sequence Viewer

Length: 498 bp
ATGATCTCCAAGGTGCAGATGAAGAGAGCGCCAGTATCCAAGAAACTGCTAACCCTTCGCTCCATCTCTAAGTCCCATGCGCACAGTAAAACCTCAATTATATTGGATGCATCAAAGTACATACAAAACTTAAAGCGAAAAGTGGAGGAAATGAATCAACAGATGATTGCCTCAGCGGAAACCTCAACTTCCCTAAACTCCTTCCCTGTGCAACTCAAAGTCGAAGCTGGGGAGAGAGGCTTTCAGATTAAGATGTTCACTGAAAAAAGATGCAGTGGGTTACTTGTTTTCATATTGGAAGCTTTTGGAGAGCTAGGCCTTAATGTGCATCAAGCTAGGGCTTCTTGTTCCAACAATTTTCTTCTAGAAGCTGTTGGAACAGTCAATGATAATCCAAGTGCTGACCAGAAAGATGTTGAGGTAGTGAAAGAAGCGATGTTGCAAGCCATTCAAAACTGGAGTGAAGTTAACCAACAACAAGAACAATGCAAGGATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

166

Amino Acids

18.46

Weight (kDa)

8.77

Isoelectric Point (pI)

33.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
bHLH-TF_ACT-like_plant PF22754 71 - 151 1.8e-20 Plant bHLH transcription factor, ACT-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 81
AciI CCGC 1 cut(s) 176
AfaI GTAC 1 cut(s) 119
AgsI TTSAA 1 cut(s) 452
AluBI AGCT 5 cut(s) 227, 302, 313, 335, 371
AluI AGCT 5 cut(s) 227, 302, 313, 335, 371
AoxI GGCC 1 cut(s) 316
AspLEI GCGC 2 cut(s) 31, 82
BbvCI CCTCAGC 1 cut(s) 172
BccI CCATC 1 cut(s) 71
BciVI GTATCC 1 cut(s) 46
BfaI CTAG 3 cut(s) 314, 336, 365
BfoI RGCGCY 1 cut(s) 32
BfuI GTATCC 1 cut(s) 46
BmsI GCATC 4 cut(s) 97, 119, 260, 337
BpmI CTGGAG 1 cut(s) 478
Bpu10I CCTNAGC 1 cut(s) 172
BsaJI CCNNGG 1 cut(s) 9
Bse1I ACTGG 2 cut(s) 32, 461
BseDI CCNNGG 1 cut(s) 9
BseGI GGATG 1 cut(s) 112
BseMII CTCAG 1 cut(s) 186
BseNI ACTGG 2 cut(s) 32, 461
BseYI CCCAGC 1 cut(s) 227
BsgI GTGCAG 1 cut(s) 35
BshFI GGCC 1 cut(s) 318
BslFI GGGAC 1 cut(s) 58
BsmFI GGGAC 1 cut(s) 58
BsnI GGCC 1 cut(s) 318
Bsp143I GATC 1 cut(s) 3
BspACI CCGC 1 cut(s) 176
BspANI GGCC 1 cut(s) 318
BspCNI CTCAG 1 cut(s) 185
BsrI ACTGG 2 cut(s) 32, 461
BssECI CCNNGG 1 cut(s) 9
BssMI GATC 1 cut(s) 3
BssT1I CCWWGG 1 cut(s) 9
Bst4CI ACNGT 2 cut(s) 86, 382
Bst6I CTCTTC 1 cut(s) 17
BstC8I GCNNGC 1 cut(s) 444
BstDEI CTNAG 2 cut(s) 69, 172
BstF5I GGATG 1 cut(s) 112
BstH2I RGCGCY 1 cut(s) 32
BstHHI GCGC 2 cut(s) 31, 82
BstKTI GATC 1 cut(s) 6
BstMBI GATC 1 cut(s) 3
BsuI GTATCC 1 cut(s) 46
BsuRI GGCC 1 cut(s) 318
BtgZI GCGATG 1 cut(s) 449
BtsCI GGATG 1 cut(s) 112
BtsI GCAGTG 1 cut(s) 280
BtsIMutI CAGTG 2 cut(s) 258, 280
Cac8I GCNNGC 1 cut(s) 444
CfoI GCGC 2 cut(s) 31, 82
Csp6I GTAC 1 cut(s) 118
CviAII CATG 1 cut(s) 77
CviJI RGCY 9 cut(s) 227, 240, 302, 313, 318, 335, 341, 371, 446
CviKI_1 RGCY 9 cut(s) 227, 240, 302, 313, 318, 335, 341, 371, 446
CviQI GTAC 1 cut(s) 118
DdeI CTNAG 2 cut(s) 69, 172
DpnI GATC 1 cut(s) 5
DpnII GATC 1 cut(s) 3
Eam1104I CTCTTC 1 cut(s) 17
EarI CTCTTC 1 cut(s) 17
Eco130I CCWWGG 1 cut(s) 9
Eco147I AGGCCT 1 cut(s) 318
EcoT14I CCWWGG 1 cut(s) 9
EcoT22I ATGCAT 1 cut(s) 112
ErhI CCWWGG 1 cut(s) 9
FaeI CATG 1 cut(s) 80
FaiI YATR 4 cut(s) 78, 101, 122, 293
FaqI GGGAC 1 cut(s) 58
FatI CATG 1 cut(s) 76
FokI GGATG 1 cut(s) 119
FspAI RTGCGCAY 1 cut(s) 81
FspBI CTAG 3 cut(s) 314, 336, 365
FspI TGCGCA 1 cut(s) 81
GlaI GCGC 2 cut(s) 30, 81
GsaI CCCAGC 1 cut(s) 231
GsuI CTGGAG 1 cut(s) 478
HaeII RGCGCY 1 cut(s) 32
HaeIII GGCC 1 cut(s) 318
HhaI GCGC 2 cut(s) 31, 82
Hin1II CATG 1 cut(s) 80
Hin6I GCGC 2 cut(s) 29, 80
HinP1I GCGC 2 cut(s) 29, 80
HincII GTYRAC 1 cut(s) 469
HindII GTYRAC 1 cut(s) 469
HindIII AAGCTT 1 cut(s) 300
HinfI GANTC 1 cut(s) 154
HpaI GTTAAC 1 cut(s) 469
Hpy166II GTNNAC 2 cut(s) 258, 469
Hpy188I TCNGA 1 cut(s) 246
Hpy188III TCNNGA 1 cut(s) 365
Hpy8I GTNNAC 2 cut(s) 258, 469
HpyAV CCTTC 2 cut(s) 65, 211
HpyCH4III ACNGT 2 cut(s) 86, 382
HpyCH4V TGCA 7 cut(s) 16, 110, 211, 273, 328, 442, 489
HpyF3I CTNAG 2 cut(s) 69, 172
Hsp92II CATG 1 cut(s) 80
HspAI GCGC 2 cut(s) 29, 80
KspAI GTTAAC 1 cut(s) 469
Kzo9I GATC 1 cut(s) 3
LmnI GCTCC 1 cut(s) 65
LpnPI CCDG 5 cut(s) 45, 213, 219, 419, 442
LweI GCATC 4 cut(s) 97, 119, 260, 337
MaeI CTAG 3 cut(s) 314, 336, 365
MaeIII GTNAC 1 cut(s) 279
MalI GATC 1 cut(s) 5
MboI GATC 1 cut(s) 3
MboII GAAGA 2 cut(s) 34, 353
MluCI AATT 2 cut(s) 96, 355
MmeI TCCRAC 2 cut(s) 355, 375
MnlI CCTC 6 cut(s) 103, 139, 181, 193, 230, 412
Mph1103I ATGCAT 1 cut(s) 112
MseI TTAA 5 cut(s) 131, 249, 321, 468, 496
MspA1I CMGCKG 1 cut(s) 176
NdeII GATC 1 cut(s) 3
NlaIII CATG 1 cut(s) 80
NsbI TGCGCA 1 cut(s) 81
NsiI ATGCAT 1 cut(s) 112
PceI AGGCCT 1 cut(s) 318
PfeI GAWTC 1 cut(s) 154
PspFI CCCAGC 1 cut(s) 227
RsaI GTAC 1 cut(s) 119
RsaNI GTAC 1 cut(s) 118
SaqAI TTAA 5 cut(s) 131, 249, 321, 468, 496
Sau3AI GATC 1 cut(s) 3
SetI ASST 9 cut(s) 15, 95, 185, 229, 304, 315, 337, 373, 423
SfaNI GCATC 4 cut(s) 97, 119, 260, 337
Sse9I AATT 2 cut(s) 96, 355
SseBI AGGCCT 1 cut(s) 318
SsiI CCGC 1 cut(s) 176
SspMI CTAG 3 cut(s) 314, 336, 365
StuI AGGCCT 1 cut(s) 318
StyI CCWWGG 1 cut(s) 9
TaaI ACNGT 2 cut(s) 86, 382
TaqI TCGA 1 cut(s) 222
TasI AATT 2 cut(s) 96, 355
TatI WGTACW 1 cut(s) 117
TfiI GAWTC 1 cut(s) 154
Tru1I TTAA 5 cut(s) 131, 249, 321, 468, 496
Tru9I TTAA 5 cut(s) 131, 249, 321, 468, 496
TscAI CASTG 2 cut(s) 265, 280
TspDTI ATGAA 3 cut(s) 35, 167, 280
TspRI CASTG 2 cut(s) 265, 280
XbaI TCTAGA 1 cut(s) 364
XspI CTAG 3 cut(s) 314, 336, 365
Zsp2I ATGCAT 1 cut(s) 112
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.