Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr03

Orthogroup ID

OG0014042

Physical Location & Seq

Genomic Coordinates Forward (+)

4740131 .. 4745445

Gene Structure 5315 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD03G1058800.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3015 bp

ATGAATCCCTCCACTCTCACTCCCCACCGCTCCGCTCTCCGCTCGCCCTTCCTCAACCCCATCTCTCTCCCCGCCGCAACCTCCGTACACTCCCGACGACGCCGTTTCAGGGTCTCATTTCCCCGGAATTCTGCCGCCTCCTCGGAACAACCAACAGATGCGTCACCGCCGCCTCCCGATGTCTTCGGCGGCAAGAGAGAGCTCACCGGTATCCAGCCCGTTGTGGAGAAGCTTTCGCCGCCGCTCCGGATAGTGACGTCGGCAATTGTGTTCGCTGGAGCTGTGGCTGCGGGTTATGGGTTGGGTCTCCGATTGGGGAAGTCCCAAAATGCAGCTTATGGCGGTGCAGCGGTGCTCGGAGCCGCCGGTGGGGCCGCCTTATACGCTATGAACTCTTGTGCTCCCGAGGTCGCCGCGGTTGACTTGCACAATTACGTTGCCGGGTTCGACGACCCGAAAGCTGTTAAGAAGGAGGACATTGAAGGCATTGCGAGAAAATATGGTGTGAGCAAGCAAGACGAGGCGTTTAATGCCGAGCTCTGTGATTTGTACTGTCGTTTTGTCACTTCTGTTCTTCCTCCTGGAGCTCAAGAACTCAAAGGCGACGAAGTCGACACAATCGTCAGCTTCAAGAATTCTCTGGGAATCGATGACCCCGAAGCAGCTTCAATGCATATGGAGATTGGAAGGCGAATTTTCAGACAGCGACTTGAGACTGACCGTGAGGGTGATCTAGAGCAGCGTCGGGCGTTTCAGAAGCTGATTTATGTTTCGACTCTTGTGTTTGGGGATGCTTCATCTTTCCTCTTACCTTGGAAGCGTGTTTTTAAAGTTACTGATTCCCAGGTTGAGCTGGCTATCCGTGATAATGCCCAGCGTTTATACGCTTCCAAGTTAAAATCCGTTGGCAGAGATATTGGAGTAGAGCAGCTTGTTAAGCTTAAAGAAGCACAACGCATGTATCGGCTCTCTGACGAGCACGCAGAGGACTTGTTCAAGGAACATGCAAGAAAACTAGTTGAGGCAAATATTTCAGCAGCACTTCGTATACTCAAGTCCAGAACCAGATCAGCTGCTGGAATCACAGAAGTTGTGGAAGAGCTCGATAAAATGCTAGAACTCAATAGTTTGCTCATCTCACTAAAGAACCAGCCAGATGCAGCTCGCTTTGCACCTGGGGTTGGCCCAGTTTCTTTACTTGGTGGCGACTACGATGCTGATAGAAAGATGGACGACTTGAAGCTCCTCTTCAGAGCATATGTTACGGATTCTTTATCCACAGGTCGCTTGGAAGAGAGTAAGCTTTCGGCCTTGAATCAGTTAAGGAATATATTTGGTTTGGGCAAACGAGAGGCAGAATCCATTGTGCTTGATGTTACTTCGAAGGTATACCGTAAATGTCTTTCACAATCTGTTGCATCGGGTGAGTTAGAAGCTGCAGACAGCAAAGCAGCCTTCCTTCAAAATATCTGTGAGGAGCTGCATTTTGATCCACAAAGAGCCAGCCAGATTCATGAAGAAATTTACCGGCAAAAGCTTCAGCAGTGTGTTGCTGATGGAGAGCTGAATGAGGATGATGTTGCTGCATTGTTAAGGCTACGGGTCATGCTTTGTATACCTCAGCAGACGGTTGAGGCTGCCCATTCAGATATCTGTGGCAGTTTGTTTGAAAAGGTTGTGAAGGACGCAATTGCATCTGGTGTTGATGGTTACGACGCTGATGTAAAACAAGCCGTGAGAAAGGCAGCTCATGGTCTGCGATTGTCTAGGGAGGCTGCCATGTCTATTGCTGGCAAGGCAGTAAGAAAGATTTTTATTAACTATGTAAAACGAGCACGGTCAGTTGGTAGCCGTACTGAGTCTGCAAAAGAACTTAAAAAGATGATAGCTTTCAACACATTGGTTGTGACTGAATTGGTGGCAGACATAAAAGGGGAATCATCTGATTCCGCCACAGAAGAGCCAATAAAGGAGCCAGAGACAGAAGTCCTGGAGGATGAGGAATGGGAATCTATTCAGACACTTCGAAAGATTAGACCCGACAAGGAGCTTGCTGCAAAGTTGGGGAAGCCTGGCCAGACTGAGATAACTCTTAAAGATGATCTGGAAGAAAGAGAGAGGACTGACCTATACAAAACATACTTGCTGTTTTGTATAACTGGGGAAGTGAAGAAGATTCCCTTTGGTGCTCAGATCACTACAAAGAAGGATGATTCAGAATATGTGCTACTAAATCAGCTTGGGGCGATATTGGGTTTAAGCACTACGGAGATAGTTGAAGTACACAGGAGCCTGGCCGAGCAAGCTTTCAGGCAACAGGCAGAGGTGATTTTAGCTGATGGACAATTGACAAAAGCCAGGGTAGAGCAACTTAATGAATTGCAGAAGCAAGTTGGCTTGCCTCCACAGTACGTGGAAAAGATAATAAAGAATATAACTACTACAAAAATGGCAGCTGCGATTGAAACCGCTATTGGTCAGGGGAGGCTCAATATTAAGCAGATAAGAGAACTTAAAGAATCAAGTGTTAATTTAGACAGCATGATATCTGAGAGCTTGCGAGAAAGTCTGTTCAAAAAGACCGTGGATGAGATATTCTCATTAGGCACCGGAGAGTTTGATGAGGAAGAAGTCTATGAGAAAATCCCAGCAGATCTCAATATTAATGCTGAGAAGACCAAGAATGTTGTTCGAGAGCTTGCACAGACCAGATTATCAAACTCACTGATTCAGGCTGTGTCACTTCTGAGGCAGAGAAATCGTCAAGGAGTGGTTTCCTCTCTTAATGACTTGCTAGCTTGCGACAAAGCTGTGCCTGCCAAGCCACTTTCATGGGAAGTTCCAGAGGAGCTGGCCGATCTCTTTGGCATATACATGAAAAGCGACGCGGCACCAGAGAAATTATCGCGCTTGCAGTATCTCTTGGGCATTAGTGACAGCATGGCCACTGCTCTCCTGGAGATGGGAGATAGATTACAAACCATCGGTGCGGAGGAGGAAAAGTTTGTATTCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006605 GO:0006810 GO:0006886 GO:0006996 GO:0008104 GO:0008150 GO:0009507 GO:0009526 GO:0009528 GO:0009536 GO:0009657 GO:0009658 GO:0009706 GO:0009941 GO:0009987 GO:0015031 GO:0015833 GO:0016020 GO:0016043 GO:0017038 GO:0019866 GO:0031090 GO:0031897 GO:0031967 GO:0031969 GO:0031975 GO:0032991 GO:0033036 GO:0033365 GO:0034613 GO:0042170 GO:0042886 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044425 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0045036 GO:0045037 GO:0045184 GO:0046907 GO:0051179 GO:0051234 GO:0051641 GO:0051649 GO:0055085 GO:0061927 GO:0065002 GO:0070727 GO:0071702 GO:0071705 GO:0071806 GO:0071840 GO:0072594 GO:0072596 GO:0072598 GO:0098796

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Tic110

Protein Analysis

1005

Amino Acids

110.85

Weight (kDa)

5.66

Isoelectric Point (pI)

44.75

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Tic110	PF16940	68 - 640	1.1e-288	Chloroplast envelope transporter

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0014042)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G06950
fragaria_vesca	FvH4_3g40080
malus_domestica	MD03G1058800.v1.1 MD11G1060900.v1.1
prunus_persica	Prupe.6G045000_v2.0.a1
pyrus_communis	pycom03g04710 pycom11g05040
rosa_chinensis	RchiOBHm_Chr5g0072271
rosa_laevigata	RLG00000036290
rosa_multiflora	Rmu_sc0000714.1_g000010
rosa_roxburghii	Rroxscaffold_1G00009000
rosa_rugosa	Rorug05G0417400
rosa_samantha	Rh5AG473200 Rh5BG493500 Rh5CG517500 Rh5DG505400
rosa_wichuraiana	Rw5G043970

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	620
AatII	GACGTC	1 cut(s)	260
AccB1I	GGYRCC	2 cut(s)	2606, 2890
AccBSI	CCGCTC	4 cut(s)	30, 35, 42, 244
AccI	GTMKAC	4 cut(s)	612, 1048, 1389, 1615
AccII	CGCG	3 cut(s)	416, 2888, 2908
AccIII	TCCGGA	1 cut(s)	246
AclWI	GGATC	1 cut(s)	1484
AcoI	YGGCCR	4 cut(s)	2074, 2295, 2853, 2943
AcsI	RAATTY	4 cut(s)	127, 634, 693, 1521
AcuI	CTGAAG	2 cut(s)	1234, 1523
AcyI	GRCGYC	2 cut(s)	100, 257
AfaI	GTAC	5 cut(s)	87, 551, 1855, 2283, 2411
AfiI	CCNNNNNNNGG	4 cut(s)	109, 369, 1181, 2962
AgeI	ACCGGT	1 cut(s)	206
AhlI	ACTAGT	1 cut(s)	1015
AjnI	CCWGG	8 cut(s)	580, 843, 1174, 1989, 2071, 2292, 2357, 2955
AjuI	GAANNNNNNNTTGG	2 cut(s)	2457, 2489
Alw21I	GWGCWC	9 cut(s)	204, 357, 403, 540, 589, 981, 1104, 1837, 2191
Alw26I	GTCTC	4 cut(s)	118, 311, 707, 1973
AlwI	GGATC	1 cut(s)	1484
AlwNI	CAGNNNCTG	2 cut(s)	760, 1076
Ama87I	CYCGRG	1 cut(s)	404
Aor13HI	TCCGGA	1 cut(s)	246
AoxI	GGCC	7 cut(s)	372, 1183, 1308, 2074, 2295, 2853, 2943
ApoI	RAATTY	4 cut(s)	127, 634, 693, 1521
AseI	ATTAAT	1 cut(s)	2664
AsiGI	ACCGGT	1 cut(s)	206
Asp700I	GAANNNNTTC	1 cut(s)	2013
AspLEI	GCGC	1 cut(s)	2910
AspS9I	GGNCC	2 cut(s)	372, 1184
AsuC2I	CCSGG	2 cut(s)	124, 442
AsuHPI	GGTGA	5 cut(s)	156, 196, 740, 1436, 2338
AsuII	TTCGAA	2 cut(s)	1382, 2026
AsuNHI	GCTAGC	1 cut(s)	2794
AvaI	CYCGRG	1 cut(s)	404
BaeI	ACNNNNGTAYC	2 cut(s)	944, 977
BalI	TGGCCA	2 cut(s)	2076, 2945
BanI	GGYRCC	2 cut(s)	2606, 2890
BanII	GRGCYC	4 cut(s)	204, 540, 589, 1104
BbsI	GAAGAC	2 cut(s)	175, 2681
Bbv12I	GWGCWC	9 cut(s)	204, 357, 403, 540, 589, 981, 1104, 1837, 2191
BbvCI	CCTCAGC	1 cut(s)	1620
BccI	CCATC	7 cut(s)	68, 1222, 1550, 1700, 2333, 2956, 2990
BceAI	ACGGC	3 cut(s)	87, 1718, 1836
BcgI	CGANNNNNNTGC	4 cut(s)	1196, 1230, 2741, 2775
BciT130I	CCWGG	8 cut(s)	582, 845, 1176, 1991, 2073, 2294, 2359, 2957
BciVI	GTATCC	1 cut(s)	221
BcnI	CCSGG	2 cut(s)	124, 442
BcoDI	GTCTC	4 cut(s)	118, 311, 707, 1973
BcuI	ACTAGT	1 cut(s)	1015
BfaI	CTAG	5 cut(s)	734, 1016, 1115, 1767, 2795
BfmI	CTRYAG	1 cut(s)	1437
BfuI	GTATCC	1 cut(s)	221
BglI	GCCNNNNNGGC	1 cut(s)	371
BglII	AGATCT	1 cut(s)	2653
BmeT110I	CYCGRG	1 cut(s)	404
BmgT120I	GGNCC	2 cut(s)	372, 1184
BmiI	GGNNCC	6 cut(s)	361, 373, 1974, 2291, 2608, 2892
BmrI	ACTGGG	2 cut(s)	1181, 2169
BmsI	GCATC	6 cut(s)	148, 781, 1147, 1204, 1427, 1703
BmtI	GCTAGC	1 cut(s)	2798
BmuI	ACTGGG	2 cut(s)	1181, 2169
BoxI	GACNNNNGTC	1 cut(s)	1985
BpiI	GAAGAC	2 cut(s)	175, 2681
BplI	GAGNNNNNCTC	2 cut(s)	2582, 2614
BpmI	CTGGAG	4 cut(s)	297, 603, 2012, 2978
Bpu10I	CCTNAGC	1 cut(s)	1620
Bpu14I	TTCGAA	2 cut(s)	1382, 2026
BpuEI	CTTGAG	3 cut(s)	573, 731, 1037
BpuMI	CCSGG	2 cut(s)	124, 442
Bsa29I	ATCGAT	1 cut(s)	648
BsaAI	YACGTR	1 cut(s)	2413
BsaHI	GRCGYC	2 cut(s)	100, 257
BsaI	GGTCTC	2 cut(s)	118, 311
BsaJI	CCNNGG	9 cut(s)	122, 141, 405, 414, 812, 843, 1175, 2358, 2583
BsaWI	WCCGGW	3 cut(s)	206, 246, 2609
BsaXI	ACNNNNNCTCC	2 cut(s)	34, 2987
Bsc4I	CCNNNNNNNGG	4 cut(s)	109, 369, 1181, 2962
Bse118I	RCCGGY	3 cut(s)	206, 365, 1527
Bse1I	ACTGG	2 cut(s)	1187, 2164
Bse3DI	GCAATG	1 cut(s)	486
BseAI	TCCGGA	1 cut(s)	246
BseBI	CCWGG	8 cut(s)	582, 845, 1176, 1991, 2073, 2294, 2359, 2957
BseCI	ATCGAT	1 cut(s)	648
BseDI	CCNNGG	9 cut(s)	122, 141, 405, 414, 812, 843, 1175, 2358, 2583
BseGI	GGATG	5 cut(s)	796, 1579, 2002, 2215, 2593
BseLI	CCNNNNNNNGG	4 cut(s)	109, 369, 1181, 2962
BseMI	GCAATG	1 cut(s)	486
BseMII	CTCAG	7 cut(s)	1634, 1848, 2073, 2204, 2541, 2661, 2738
BseNI	ACTGG	2 cut(s)	1187, 2164
BseRI	GAGGAG	5 cut(s)	130, 1235, 1490, 2861, 3008
BseYI	CCCAGC	2 cut(s)	873, 2647
BsgI	GTGCAG	1 cut(s)	366
Bsh1236I	CGCG	3 cut(s)	416, 2888, 2908
BshFI	GGCC	7 cut(s)	374, 1185, 1310, 2076, 2297, 2855, 2945
BshNI	GGYRCC	2 cut(s)	2606, 2890
BshTI	ACCGGT	1 cut(s)	206
BshVI	ATCGAT	1 cut(s)	648
BsiHKAI	GWGCWC	9 cut(s)	204, 357, 403, 540, 589, 981, 1104, 1837, 2191
BsiHKCI	CYCGRG	1 cut(s)	404
BsiSI	CCGG	7 cut(s)	124, 207, 247, 366, 441, 1528, 2610
BslFI	GGGAC	1 cut(s)	307
BslI	CCNNNNNNNGG	4 cut(s)	109, 369, 1181, 2962
BsmAI	GTCTC	4 cut(s)	118, 311, 707, 1973
BsmFI	GGGAC	1 cut(s)	307
BsnI	GGCC	7 cut(s)	374, 1185, 1310, 2076, 2297, 2855, 2945
Bso31I	GGTCTC	2 cut(s)	118, 311
BsoBI	CYCGRG	1 cut(s)	404
Bsp119I	TTCGAA	2 cut(s)	1382, 2026
Bsp1286I	GDGCHC	9 cut(s)	204, 357, 403, 540, 589, 981, 1104, 1837, 2191
Bsp13I	TCCGGA	1 cut(s)	246
Bsp143I	GATC	7 cut(s)	730, 1067, 1489, 2101, 2193, 2653, 2857
BspANI	GGCC	7 cut(s)	374, 1185, 1310, 2076, 2297, 2855, 2945
BspCNI	CTCAG	7 cut(s)	1633, 1849, 2074, 2203, 2542, 2662, 2739
BspDI	ATCGAT	1 cut(s)	648
BspEI	TCCGGA	1 cut(s)	246
BspFNI	CGCG	3 cut(s)	416, 2888, 2908
BspHI	TCATGA	1 cut(s)	1513
BspLI	GGNNCC	6 cut(s)	361, 373, 1974, 2291, 2608, 2892
BspMAI	CTGCAG	1 cut(s)	1441
BspOI	GCTAGC	1 cut(s)	2798
BspPI	GGATC	1 cut(s)	1484
BspQI	GCTCTTC	2 cut(s)	1092, 1953
BspT104I	TTCGAA	2 cut(s)	1382, 2026
BspT107I	GGYRCC	2 cut(s)	2606, 2890
BspTNI	GGTCTC	2 cut(s)	118, 311
BsrBI	CCGCTC	4 cut(s)	30, 35, 42, 244
BsrDI	GCAATG	1 cut(s)	486
BsrFI	RCCGGY	3 cut(s)	206, 365, 1527
BsrI	ACTGG	2 cut(s)	1187, 2164
BssAI	RCCGGY	3 cut(s)	206, 365, 1527
BssECI	CCNNGG	9 cut(s)	122, 141, 405, 414, 812, 843, 1175, 2358, 2583
BssMI	GATC	7 cut(s)	730, 1067, 1489, 2101, 2193, 2653, 2857
BssNAI	GTATAC	3 cut(s)	1049, 1390, 1616
BssNI	GRCGYC	2 cut(s)	100, 257
BssT1I	CCWWGG	1 cut(s)	812
Bst1107I	GTATAC	3 cut(s)	1049, 1390, 1616
Bst2UI	CCWGG	8 cut(s)	582, 845, 1176, 1991, 2073, 2294, 2359, 2957
Bst4CI	ACNGT	7 cut(s)	554, 722, 1394, 1630, 1839, 2409, 2584
Bst6I	CTCTTC	4 cut(s)	1092, 1253, 1287, 1953
BstACI	GRCGYC	2 cut(s)	100, 257
BstBAI	YACGTR	1 cut(s)	2413
BstBI	TTCGAA	2 cut(s)	1382, 2026
BstDEI	CTNAG	7 cut(s)	1620, 1857, 2082, 2190, 2550, 2670, 2747
BstDSI	CCRYGG	2 cut(s)	414, 2583
BstF5I	GGATG	5 cut(s)	796, 1579, 2002, 2215, 2593
BstFNI	CGCG	3 cut(s)	416, 2888, 2908
BstHHI	GCGC	1 cut(s)	2910
BstKTI	GATC	7 cut(s)	733, 1070, 1492, 2104, 2196, 2656, 2860
BstMAI	GTCTC	4 cut(s)	118, 311, 707, 1973
BstMBI	GATC	7 cut(s)	730, 1067, 1489, 2101, 2193, 2653, 2857
BstNI	CCWGG	8 cut(s)	582, 845, 1176, 1991, 2073, 2294, 2359, 2957
BstNSI	RCATGY	2 cut(s)	961, 1007
BstPAI	GACNNNNGTC	1 cut(s)	1985
BstSFI	CTRYAG	1 cut(s)	1437
BstUI	CGCG	3 cut(s)	416, 2888, 2908
BstV2I	GAAGAC	2 cut(s)	175, 2681
BstX2I	RGATCY	1 cut(s)	2653
BstXI	CCANNNNNNTGG	1 cut(s)	2832
BstYI	RGATCY	1 cut(s)	2653
BstZ17I	GTATAC	3 cut(s)	1049, 1390, 1616
Bsu15I	ATCGAT	1 cut(s)	648
BsuI	GTATCC	1 cut(s)	221
BsuRI	GGCC	7 cut(s)	374, 1185, 1310, 2076, 2297, 2855, 2945
BsuTUI	ATCGAT	1 cut(s)	648
BtgI	CCRYGG	2 cut(s)	414, 2583
BtsCI	GGATG	5 cut(s)	796, 1579, 2002, 2215, 2593
BtsI	GCAGTG	2 cut(s)	1550, 2946
BtsIMutI	CAGTG	3 cut(s)	1550, 2723, 2946
CaiI	CAGNNNCTG	2 cut(s)	760, 1076
CciI	TCATGA	1 cut(s)	1513
CfoI	GCGC	1 cut(s)	2910
Cfr10I	RCCGGY	3 cut(s)	206, 365, 1527
Cfr13I	GGNCC	2 cut(s)	372, 1184
Cfr42I	CCGCGG	1 cut(s)	417
ClaI	ATCGAT	1 cut(s)	648
CseI	GACGC	6 cut(s)	108, 150, 731, 1694, 1724, 2894
Csp6I	GTAC	5 cut(s)	86, 550, 1854, 2282, 2410
CspAI	ACCGGT	1 cut(s)	206
CviQI	GTAC	5 cut(s)	86, 550, 1854, 2282, 2410
DdeI	CTNAG	7 cut(s)	1620, 1857, 2082, 2190, 2550, 2670, 2747
DpnI	GATC	7 cut(s)	732, 1069, 1491, 2103, 2195, 2655, 2859
DpnII	GATC	7 cut(s)	730, 1067, 1489, 2101, 2193, 2653, 2857
DraI	TTTAAA	1 cut(s)	829
DrdI	GACNNNNNNGTC	1 cut(s)	620
DseDI	GACNNNNNNGTC	1 cut(s)	620
EaeI	YGGCCR	4 cut(s)	2074, 2295, 2853, 2943
Eam1104I	CTCTTC	4 cut(s)	1092, 1253, 1287, 1953
EarI	CTCTTC	4 cut(s)	1092, 1253, 1287, 1953
EciI	GGCGGA	1 cut(s)	1939
Ecl136II	GAGCTC	4 cut(s)	202, 538, 587, 1102
Eco130I	CCWWGG	1 cut(s)	812
Eco24I	GRGCYC	4 cut(s)	204, 540, 589, 1104
Eco31I	GGTCTC	2 cut(s)	118, 311
Eco32I	GATATC	2 cut(s)	1651, 2547
Eco53kI	GAGCTC	4 cut(s)	202, 538, 587, 1102
Eco57I	CTGAAG	2 cut(s)	1234, 1523
Eco88I	CYCGRG	1 cut(s)	404
EcoICRI	GAGCTC	4 cut(s)	202, 538, 587, 1102
EcoRI	GAATTC	2 cut(s)	127, 634
EcoRII	CCWGG	8 cut(s)	580, 843, 1174, 1989, 2071, 2292, 2357, 2955
EcoRV	GATATC	2 cut(s)	1651, 2547
EcoT14I	CCWWGG	1 cut(s)	812
EcoT22I	ATGCAT	1 cut(s)	675
EcoT38I	GRGCYC	4 cut(s)	204, 540, 589, 1104
ErhI	CCWWGG	1 cut(s)	812
FalI	AAGNNNNNCTT	4 cut(s)	1232, 1264, 2165, 2197
FaqI	GGGAC	1 cut(s)	307
FauI	CCCGC	2 cut(s)	79, 283
FauNDI	CATATG	2 cut(s)	675, 1258
FblI	GTMKAC	4 cut(s)	612, 1048, 1389, 1615
FokI	GGATG	5 cut(s)	803, 1586, 2009, 2222, 2600
FriOI	GRGCYC	4 cut(s)	204, 540, 589, 1104
FspBI	CTAG	5 cut(s)	734, 1016, 1115, 1767, 2795
GlaI	GCGC	1 cut(s)	2909
GsaI	CCCAGC	2 cut(s)	877, 2651
GsuI	CTGGAG	4 cut(s)	297, 603, 2012, 2978
HaeIII	GGCC	7 cut(s)	374, 1185, 1310, 2076, 2297, 2855, 2945
HapII	CCGG	7 cut(s)	124, 207, 247, 366, 441, 1528, 2610
HgaI	GACGC	6 cut(s)	108, 150, 731, 1694, 1724, 2894
HhaI	GCGC	1 cut(s)	2910
Hin1I	GRCGYC	2 cut(s)	100, 257
Hin6I	GCGC	1 cut(s)	2908
HinP1I	GCGC	1 cut(s)	2908
HincII	GTYRAC	2 cut(s)	421, 613
HindII	GTYRAC	2 cut(s)	421, 613
HindIII	AAGCTT	5 cut(s)	230, 938, 1301, 1535, 2304
HpaII	CCGG	7 cut(s)	124, 207, 247, 366, 441, 1528, 2610
HphI	GGTGA	5 cut(s)	156, 196, 740, 1436, 2338
Hpy166II	GTNNAC	7 cut(s)	88, 421, 613, 1049, 1390, 1616, 2284
Hpy8I	GTNNAC	7 cut(s)	88, 421, 613, 1049, 1390, 1616, 2284
Hpy99I	CGWCG	8 cut(s)	99, 102, 262, 452, 608, 747, 1718, 2888
HpyAV	CCTTC	9 cut(s)	58, 463, 476, 681, 1378, 1465, 1469, 1675, 2200
HpyCH4III	ACNGT	7 cut(s)	554, 722, 1394, 1630, 1839, 2409, 2584
HpyCH4IV	ACGT	3 cut(s)	257, 435, 2412
HpyF3I	CTNAG	7 cut(s)	1620, 1857, 2082, 2190, 2550, 2670, 2747
HpySE526I	ACGT	3 cut(s)	257, 435, 2412
Hsp92I	GRCGYC	2 cut(s)	100, 257
HspAI	GCGC	1 cut(s)	2908
Kpn2I	TCCGGA	1 cut(s)	246
KspI	CCGCGG	1 cut(s)	417
Kzo9I	GATC	7 cut(s)	730, 1067, 1489, 2101, 2193, 2653, 2857
LguI	GCTCTTC	2 cut(s)	1092, 1953
LweI	GCATC	6 cut(s)	148, 781, 1147, 1204, 1427, 1703
MaeI	CTAG	5 cut(s)	734, 1016, 1115, 1767, 2795
MaeII	ACGT	3 cut(s)	257, 435, 2412
MalI	GATC	7 cut(s)	732, 1069, 1491, 2103, 2195, 2655, 2859
MbiI	CCGCTC	4 cut(s)	30, 35, 42, 244
MboI	GATC	7 cut(s)	730, 1067, 1489, 2101, 2193, 2653, 2857
MfeI	CAATTG	3 cut(s)	264, 1689, 2345
MflI	RGATCY	1 cut(s)	2653
MhlI	GDGCHC	9 cut(s)	204, 357, 403, 540, 589, 981, 1104, 1837, 2191
MlsI	TGGCCA	2 cut(s)	2076, 2945
MluNI	TGGCCA	2 cut(s)	2076, 2945
MlyI	GAGTC	2 cut(s)	769, 1868
Mox20I	TGGCCA	2 cut(s)	2076, 2945
Mph1103I	ATGCAT	1 cut(s)	675
MroI	TCCGGA	1 cut(s)	246
MroXI	GAANNNNTTC	1 cut(s)	2013
MscI	TGGCCA	2 cut(s)	2076, 2945
MslI	CAYNNNNRTG	2 cut(s)	2830, 2873
Msp20I	TGGCCA	2 cut(s)	2076, 2945
MspA1I	CMGCKG	4 cut(s)	350, 416, 1073, 2456
MspI	CCGG	7 cut(s)	124, 207, 247, 366, 441, 1528, 2610
MunI	CAATTG	3 cut(s)	264, 1689, 2345
MvaI	CCWGG	8 cut(s)	582, 845, 1176, 1991, 2073, 2294, 2359, 2957
MvnI	CGCG	3 cut(s)	416, 2888, 2908
NciI	CCSGG	2 cut(s)	124, 442
NdeI	CATATG	2 cut(s)	675, 1258
NdeII	GATC	7 cut(s)	730, 1067, 1489, 2101, 2193, 2653, 2857
NheI	GCTAGC	1 cut(s)	2794
NlaIV	GGNNCC	6 cut(s)	361, 373, 1974, 2291, 2608, 2892
NmeAIII	GCCGAG	2 cut(s)	559, 2323
NmuCI	GTSAC	6 cut(s)	162, 253, 562, 1906, 2739, 2933
NsiI	ATGCAT	1 cut(s)	675
NspI	RCATGY	2 cut(s)	961, 1007
NspV	TTCGAA	2 cut(s)	1382, 2026
PagI	TCATGA	1 cut(s)	1513
PciSI	GCTCTTC	2 cut(s)	1092, 1953
PcsI	WCGNNNNNNNCGW	2 cut(s)	618, 654
PdmI	GAANNNNTTC	1 cut(s)	2013
PflFI	GACNNNGTC	1 cut(s)	608
PfoI	TCCNGGA	3 cut(s)	580, 1989, 2955
PinAI	ACCGGT	1 cut(s)	206
PleI	GAGTC	2 cut(s)	769, 1867
PpsI	GAGTC	2 cut(s)	769, 1867
Ppu21I	YACGTR	1 cut(s)	2413
PshAI	GACNNNNGTC	1 cut(s)	1985
PshBI	ATTAAT	1 cut(s)	2664
Psp124BI	GAGCTC	4 cut(s)	204, 540, 589, 1104
Psp6I	CCWGG	8 cut(s)	580, 843, 1174, 1989, 2071, 2292, 2357, 2955
PspFI	CCCAGC	2 cut(s)	873, 2647
PspGI	CCWGG	8 cut(s)	580, 843, 1174, 1989, 2071, 2292, 2357, 2955
PspN4I	GGNNCC	6 cut(s)	361, 373, 1974, 2291, 2608, 2892
PspPI	GGNCC	2 cut(s)	372, 1184
PstI	CTGCAG	1 cut(s)	1441
PstNI	CAGNNNCTG	2 cut(s)	760, 1076
PsuI	RGATCY	1 cut(s)	2653
PsyI	GACNNNGTC	1 cut(s)	608
PvuII	CAGCTG	2 cut(s)	1073, 2456
RsaI	GTAC	5 cut(s)	87, 551, 1855, 2283, 2411
RsaNI	GTAC	5 cut(s)	86, 550, 1854, 2282, 2410
RseI	CAYNNNNRTG	2 cut(s)	2830, 2873
SacI	GAGCTC	4 cut(s)	204, 540, 589, 1104
SacII	CCGCGG	1 cut(s)	417
SalI	GTCGAC	1 cut(s)	611
SapI	GCTCTTC	2 cut(s)	1092, 1953
Sau3AI	GATC	7 cut(s)	730, 1067, 1489, 2101, 2193, 2653, 2857
Sau96I	GGNCC	2 cut(s)	372, 1184
SchI	GAGTC	2 cut(s)	769, 1868
SduI	GDGCHC	9 cut(s)	204, 357, 403, 540, 589, 981, 1104, 1837, 2191
SfaNI	GCATC	6 cut(s)	148, 781, 1147, 1204, 1427, 1703
SfcI	CTRYAG	1 cut(s)	1437
Sfr303I	CCGCGG	1 cut(s)	417
SfuI	TTCGAA	2 cut(s)	1382, 2026
SgrAI	CRCCGGYG	1 cut(s)	365
SgrBI	CCGCGG	1 cut(s)	417
SmiMI	CAYNNNNRTG	2 cut(s)	2830, 2873
SmlI	CTYRAG	3 cut(s)	588, 710, 1052
SmoI	CTYRAG	3 cut(s)	588, 710, 1052
SpeI	ACTAGT	1 cut(s)	1015
SspI	AATATT	3 cut(s)	1030, 2494, 2662
SspMI	CTAG	5 cut(s)	734, 1016, 1115, 1767, 2795
SstI	GAGCTC	4 cut(s)	204, 540, 589, 1104
StyI	CCWWGG	1 cut(s)	812
TaaI	ACNGT	7 cut(s)	554, 722, 1394, 1630, 1839, 2409, 2584
TaiI	ACGT	3 cut(s)	260, 438, 2415
TaqI	TCGA	8 cut(s)	447, 612, 648, 773, 1104, 1382, 2026, 2692
TatI	WGTACW	2 cut(s)	549, 2281
TscAI	CASTG	3 cut(s)	1550, 2730, 2953
TseFI	GTSAC	6 cut(s)	162, 253, 562, 1906, 2739, 2933
Tsp45I	GTSAC	6 cut(s)	162, 253, 562, 1906, 2739, 2933
TspDTI	ATGAA	8 cut(s)	17, 404, 786, 1502, 1530, 2391, 2820, 2891
TspGWI	ACGGA	5 cut(s)	73, 851, 892, 1280, 2282
TspRI	CASTG	3 cut(s)	1550, 2730, 2953
Tth111I	GACNNNGTC	1 cut(s)	608
VspI	ATTAAT	1 cut(s)	2664
XapI	RAATTY	4 cut(s)	127, 634, 693, 1521
XbaI	TCTAGA	1 cut(s)	733
XceI	RCATGY	2 cut(s)	961, 1007
XcmI	CCANNNNNNNNNTGG	2 cut(s)	1285, 2953
XmiI	GTMKAC	4 cut(s)	612, 1048, 1389, 1615
XmnI	GAANNNNTTC	1 cut(s)	2013
XspI	CTAG	5 cut(s)	734, 1016, 1115, 1767, 2795
ZraI	GACGTC	1 cut(s)	258
Zsp2I	ATGCAT	1 cut(s)	675

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.