Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr11

Orthogroup ID

OG0014042

Physical Location & Seq

Genomic Coordinates Forward (+)

5300211 .. 5308724

Gene Structure 8514 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD11G1060900.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3984 bp

ATGAATCCCTCCACTCTCACTTCCCAGCGCTCCTCTCTCCGCTCGCCTTTCCTCAACCCCATCTCTCTCCCCGCCGCAACCTCCGTACAATCCCGACGACGCCCTTTCAGGGTCTCATTTTCCCGGAACTCTGCCGCCCCCTCGGAACAATCAACAGACGCCGCCTCATCGCCACCTGCCGATGTCTTCGGCGGTAAGAGAGAGCTCACCGGCGTACAGCCCGTTGTCGAGAAGCTGACGCCGCCGCTCCGGATAGTGACCTCGGCAATTGTTTTTGCCGGAGCTGTGGCTGCTGGATACGGGTTGGGTCTCCGGTTGGGGAAATCCCAAAACGCAGCGTATGGTGGTGCAGCGGTGCTCGGAGCCGCCGGTGGGGCAGCAGTCTACGCTTTGAACTCTTGCGCTCCCGAGGTCGCCGCCGTCAACTTGCACAATTACGTTGCTGGGTTGGACGACCCGAAAGCTGTTAAGAAGGAGGACATCGAAGGCATTGCGAGAAAATATGGTGTGAGCAAGCAAGACGAGGCGTTTAATGCCGAGCTCTGTGGTTTGTACTGTCGTTTTGTCACTTCTGTTCTTCCTCCTGGAGCTCAAGAACTCAAAGGCGATGAAGTGGATACCATCGTCAGCTTCAAGAATTCTCTGGGAATCGATGACCCTGAAGCAGCTTCAATGCATATGGAGATTGGTAGGCGAATTTTCAGACAGAGGCTTGAGACTGACCGCGAGGGTGATCTAGAGCAGCGTCGGGCATTTCAGAAGCTGATATATGTTTCGACTCTTGTGTTTGGGGACGCTTCATCTTTCCTCTTACCTTGGAAGCGTGTCTTCAAAGTTACCGATTCCCAGGTTGAGCTGGCTATCCGTGACAATGCCCAGCGTTTATATGCTTCCAAGCTAAAATCTGTTGGCAGAGATATTGGCGCAGAGCAGCTTGTTAAGCTTAAGGAAGCACAACGCATTTATCGGCTCTCTGATGAGCACGCAGAAGACTTGTTCAAAGAACACGCAAGAAAACTAGTTGAGGCAAATATATCAGCAGCACTTCGTATACTCAAATCCAGAACCAGATCAGCCACTGGAATCACAGAAGTTGTGGAAGAGCTTGATAAAATGCTAGAACTCAATAATTTGCTCATCTCACTAAAGAACCATCCAGATGCAGCTCGCTTTGCACCTGGGCTTGGGGCAGTTTCTTTACATGGTGGCGACTACGATGCTGATAGAAAGATGGACGACTTGAAGCTCCTCTTCAGAGCATATGTTACAGATACTTTATCCACAGGTCGCTTGGAAGAGAGTAAGCATTCCGCGTTGAATCAGTTAAGGAATATATTTGGTTTAGGCAAACGAGAGGCAGAATCCATTGTGCTTGATGTTACTTCGAAGGTTTACCGTAAACGTCTTTCACAGTCTGTTACATCGGGTGATTTAGAAGCAGCGGACAGCAAAGCAGCCTTCCTTCAAAATATCTGTGAGGAGTTGCATTTTGATCCACAAAGAGCCAGCCAGATTCATGAAGAAATATACCGACAAAAGCTTCAGCAGTGTGTTGCTGATGGAGAGCTGAATGATGATGATGTTGCTGCATTGTTAAGGCTACGGGTCATGCTTTGTATTCCTCAGCAGACTATTGAGGCTGCCCATTCAGATATCTGTGGCAGTTTGTTTGAAAAGGTTGTGAAGGATGCAATTGCATCTGGTGTTGATGGTTACGACGCTGATGTAAAACAAGCTGTGAGAAAGGCAGCTCACGGTCTGCGATTGTCTAGAGAGGCTGCCATGTCTATTGCTGGCAAGGCAGTAAGAAAGATTTTTATTAACTATGTAAAACGAGCGCGGTCAGTTGGTAGCCGTACTGAGTCTGCAAAAGAACTTAAAAAGATGATAGCCTTCAACACATTGGTTGTGACTGAATTGGTGGCAGACATAAAAGGGGAATCATCTGATTCCGCCACAGAAGAGCCAATAAAGGAGCCAGAGCCAGAGGTCCTGGAGGATGAGGAATGGGAATCTATTCAGACACTTAGAAAGATTAGACCTGACAAGGAGCTTGCTGCAAAGTTGGGGAAGCCCGGCCAGACTGAGATTACTCTTAAAGATGACCTGGAAGAAAGAGAGAGGACTGACCTATACAAAACATACTTATTGTTTTGTATAACTGGGGAAGTGAAGAGGATTCCGTTTGGTGCTCAGATCACTACAAAGAAGGATGATTCAGAATATGTGCTACTAAATCAGCTTGGGGCGATATTGGGCTTAAGCACTACGGAGATAGTGGAAGTACACAGGAGCCTGGCCGAGCAAGCTTTCAGGCAACAGGCAGAGGTAATTTTAGCCGATGGACAATTGACAAAAGCAAGGGTAGAGCAACTTAATGAATTGCAGAAGCAAGTTGGCTTGCCTCCTCAGTATGTGGAAAAGATAATAAAGAATATAACTACTACAAAAATGGCAGCTGCGATTGAAACGGCTATTGGTCAGGGGAGGCTTAATATTAAGCAGATAAGAGAACTTAAAGAATCAAGTGTTAATTTAGACAGCATGATATCTGAGAGCTTGCGAGAAAGTCTGTTTAAAAAGACCGTGGATGAGATATTCTCATCGGATCTCAATATTAATGCTGAGAAGGCCAAGAATGTTGTTCGAGAGCTTGCACAGACCAGATTATCAAACTCATTGATTCAAGCTGTGTCACTTCTGAGGCAGAGAAATCGTCAAGGAGTGGTATCCTCTCTTAATGACTTGCTGGCTTGTGACAAAGCTGTGCCTGCCAAGCCACTTTCATGGGATGTTCCAGAGGAGCTGGCCGATCTCTTCGCTATATACATGAAAAGTGGCGCGGCACCTGAGAAATTATCGCGCCTGCAGTATCTCCTGGGCATTAGTGATAGCATGGCAGCTACTCTCCAGGAGATGGGAGATAGATTACAAACCATCGGTGCGGAGGAGGAAAAGTTTGTCCATTCTACTGGAGAAAGAAAAAAGGAAAAGAAGTACATTGTACCCTCTATCTTTTTGGCTATGGCTTTTATCTCACGCTATATCAGATTCTGCTTCTTTGTGCTTTTATTCCTGTTTAGGATATCGTCTGCCCAATTGTCCTCCAATTACTACTCGACAACGTGCCCTCGAACCCTTTCCATTGTTCGGAATGCAGTTGTTAATGCAGTGGTGAAGGAGCATCGCATGGGGGCATCCTTACTCCGTCTCCATTTCCACGATTGCTTTGTCAATGGATGTGATGCGTCTGTTCTATTAGATGACACTGCAAATTTTACTGGGGAAAAGACGGCGCCACCAAATACAAACTCATTGAGGGGATTTGAATTGATCGATACCATAAAATCTCAGCTAGAAGGTGCCTGCCCCGGGGTTGTTTCCTGCGCAGATATACTTGCCATTGCAGCTCGCGATTCAGTTGTTTCATTGGGAGGGCCTTCATGGACGGTTCAGTTGGGCAGAAGGGACTCCACCACAGCAAGTTTAAGTGCTGCAACTAGTGAGCTCCCATCTCCAAGTCTAAATCTTAATGACCTCATCACTTCTTTCTCAACTAAAGGATTTACTACCAAAGAATTGGTAGCTCTCTCGGGATCTCACACAACGGGCCAAGCAAGGTGTCTAGTGTTTCGAGAACGGATACATAATGAGACTAACATCGATTCATCATTTGCTACATCTTTGAAATCAAACTGTACTGCGAGCTCGGGGACTGATGACAACCTTTCTTCACTAGACGTCACAAGCCCTTTCATCTTCGATAATGCTTATTATAAGAACCTGGTGAATAGCAAGGGTCTAATGCATTCTGATCAGCAGCTTTTCAGTGGTGGCTCAACAGATTCTCTGGTTAAAACTTATAGCACCACTGCCGAAACTTTTTATACAGATTTTGCAAATGCCATGCTGAAAATGGGGAGCCTTAGCCCGCTTACGGGAAAGAGTGGTCAAGTTCGTACCAACTGTCGCAAAATAAACTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006605 GO:0006810 GO:0006886 GO:0006996 GO:0008104 GO:0008150 GO:0009507 GO:0009526 GO:0009528 GO:0009536 GO:0009657 GO:0009658 GO:0009706 GO:0009941 GO:0009987 GO:0015031 GO:0015833 GO:0016020 GO:0016043 GO:0017038 GO:0019866 GO:0031090 GO:0031897 GO:0031967 GO:0031969 GO:0031975 GO:0032991 GO:0033036 GO:0033365 GO:0034613 GO:0042170 GO:0042886 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044425 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0045036 GO:0045037 GO:0045184 GO:0046907 GO:0051179 GO:0051234 GO:0051641 GO:0051649 GO:0055085 GO:0061927 GO:0065002 GO:0070727 GO:0071702 GO:0071705 GO:0071806 GO:0071840 GO:0072594 GO:0072596 GO:0072598 GO:0098796

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Tic110

Protein Analysis

1328

Amino Acids

145.61

Weight (kDa)

7.04

Isoelectric Point (pI)

42.7

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Tic110	PF16940	69 - 641	4e-289	Chloroplast envelope transporter
peroxidase	PF00141	1050 - 1289	2.3e-83	Peroxidase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0014042)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G06950
fragaria_vesca	FvH4_3g40080
malus_domestica	MD03G1058800.v1.1 MD11G1060900.v1.1
prunus_persica	Prupe.6G045000_v2.0.a1
pyrus_communis	pycom03g04710 pycom11g05040
rosa_chinensis	RchiOBHm_Chr5g0072271
rosa_laevigata	RLG00000036290
rosa_multiflora	Rmu_sc0000714.1_g000010
rosa_roxburghii	Rroxscaffold_1G00009000
rosa_rugosa	Rorug05G0417400
rosa_samantha	Rh5AG473200 Rh5BG493500 Rh5CG517500 Rh5DG505400
rosa_wichuraiana	Rw5G043970

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	3777
AarI	CACCTGC	1 cut(s)	184
AatII	GACGTC	1 cut(s)	3744
Acc16I	TGCGCA	1 cut(s)	3388
Acc36I	ACCTGC	1 cut(s)	184
AccB1I	GGYRCC	3 cut(s)	2845, 3295, 3362
AccB7I	CCANNNNNTGG	1 cut(s)	2917
AccBSI	CCGCTC	2 cut(s)	42, 247
AccI	GTMKAC	2 cut(s)	384, 1051
AccII	CGCG	6 cut(s)	726, 1313, 1840, 2843, 2863, 3414
AccIII	TCCGGA	1 cut(s)	249
AclWI	GGATC	3 cut(s)	1487, 2616, 3604
AcoI	YGGCCR	3 cut(s)	2077, 2298, 2808
AcsI	RAATTY	3 cut(s)	637, 696, 3274
AcuI	CTGAAG	3 cut(s)	681, 1237, 1526
AcyI	GRCGYC	5 cut(s)	100, 159, 239, 3296, 3741
AfaI	GTAC	9 cut(s)	87, 216, 554, 1858, 2286, 2999, 3006, 3700, 3961
AfeI	AGCGCT	1 cut(s)	29
AfiI	CCNNNNNNNGG	8 cut(s)	109, 372, 1184, 2917, 3150, 3372, 3937, 3938
AflII	CTTAAG	2 cut(s)	944, 2260
AhlI	ACTAGT	2 cut(s)	1018, 3500
AjnI	CCWGG	9 cut(s)	583, 846, 1177, 1992, 2106, 2295, 2877, 2910, 3783
AjuI	GAANNNNNNNTTGG	2 cut(s)	2460, 2492
Alw21I	GWGCWC	8 cut(s)	207, 360, 543, 592, 984, 2194, 3510, 3710
Alw26I	GTCTC	5 cut(s)	118, 314, 710, 3215, 3647
AlwI	GGATC	3 cut(s)	1487, 2616, 3604
AlwNI	CAGNNNCTG	3 cut(s)	763, 1079, 3054
Ama87I	CYCGRG	4 cut(s)	407, 3371, 3592, 3709
Aor13HI	TCCGGA	1 cut(s)	249
Aor51HI	AGCGCT	1 cut(s)	29
AoxI	GGCC	6 cut(s)	2077, 2298, 2631, 2808, 3437, 3610
ApoI	RAATTY	3 cut(s)	637, 696, 3274
AseI	ATTAAT	1 cut(s)	2619
Asp700I	GAANNNNTTC	2 cut(s)	2016, 3139
AspLEI	GCGC	8 cut(s)	30, 404, 926, 1840, 2843, 2865, 3298, 3389
AspS9I	GGNCC	3 cut(s)	1990, 3437, 3610
AsuC2I	CCSGG	4 cut(s)	124, 2076, 3372, 3373
AsuHPI	GGTGA	5 cut(s)	199, 743, 1439, 3187, 3799
AsuII	TTCGAA	1 cut(s)	1385
AvaI	CYCGRG	4 cut(s)	407, 3371, 3592, 3709
AvaII	GGWCC	1 cut(s)	1990
BaeGI	GKGCMC	1 cut(s)	3131
BaeI	ACNNNNGTAYC	2 cut(s)	609, 642
BanI	GGYRCC	3 cut(s)	2845, 3295, 3362
BanII	GRGCYC	5 cut(s)	207, 543, 592, 3510, 3710
BbsI	GAAGAC	3 cut(s)	178, 820, 996
Bbv12I	GWGCWC	8 cut(s)	207, 360, 543, 592, 984, 2194, 3510, 3710
BbvCI	CCTCAGC	1 cut(s)	1623
BceAI	ACGGC	4 cut(s)	404, 1839, 2487, 3309
BcgI	CGANNNNNNTGC	6 cut(s)	473, 507, 1199, 1233, 2696, 2730
BciT130I	CCWGG	9 cut(s)	585, 848, 1179, 1994, 2108, 2297, 2879, 2912, 3785
BciVI	GTATCC	4 cut(s)	290, 610, 2740, 3636
BclI	TGATCA	1 cut(s)	3814
BcnI	CCSGG	4 cut(s)	124, 2076, 3372, 3373
BcoDI	GTCTC	5 cut(s)	118, 314, 710, 3215, 3647
BcuI	ACTAGT	2 cut(s)	1018, 3500
BfaI	CTAG	8 cut(s)	737, 1019, 1118, 1770, 3356, 3501, 3626, 3737
BfmI	CTRYAG	1 cut(s)	2867
BfoI	RGCGCY	2 cut(s)	31, 3299
BfrI	CTTAAG	2 cut(s)	944, 2260
BfuAI	ACCTGC	1 cut(s)	184
BfuI	GTATCC	4 cut(s)	290, 610, 2740, 3636
BglI	GCCNNNNNGGC	1 cut(s)	374
Bme18I	GGWCC	1 cut(s)	1990
BmeT110I	CYCGRG	4 cut(s)	407, 3371, 3592, 3709
BmgT120I	GGNCC	3 cut(s)	1990, 3437, 3610
BmiI	GGNNCC	7 cut(s)	364, 1977, 2294, 2847, 3297, 3364, 3923
BmrI	ACTGGG	2 cut(s)	2172, 3291
BmsI	GCATC	7 cut(s)	1150, 1207, 1678, 1706, 3193, 3206, 3235
BmuI	ACTGGG	2 cut(s)	2172, 3291
BpiI	GAAGAC	3 cut(s)	178, 820, 996
BplI	GAGNNNNNCTC	2 cut(s)	2585, 2617
BpmI	CTGGAG	4 cut(s)	606, 2015, 2894, 2994
Bpu10I	CCTNAGC	2 cut(s)	1623, 3926
Bpu14I	TTCGAA	1 cut(s)	1385
BpuEI	CTTGAG	2 cut(s)	576, 734
BpuMI	CCSGG	4 cut(s)	124, 2076, 3372, 3373
Bsa29I	ATCGAT	3 cut(s)	651, 3336, 3663
BsaHI	GRCGYC	5 cut(s)	100, 159, 239, 3296, 3741
BsaI	GGTCTC	2 cut(s)	118, 314
BsaWI	WCCGGW	2 cut(s)	249, 312
BsaXI	ACNNNNNCTCC	4 cut(s)	2942, 2972, 3195, 3225
Bsc4I	CCNNNNNNNGG	8 cut(s)	109, 372, 1184, 2917, 3150, 3372, 3937, 3938
Bse118I	RCCGGY	2 cut(s)	209, 368
Bse1I	ACTGG	4 cut(s)	1084, 2167, 2977, 3286
Bse3DI	GCAATG	2 cut(s)	489, 3402
BseAI	TCCGGA	1 cut(s)	249
BseBI	CCWGG	9 cut(s)	585, 848, 1179, 1994, 2108, 2297, 2879, 2912, 3785
BseCI	ATCGAT	3 cut(s)	651, 3336, 3663
BseGI	GGATG	8 cut(s)	1153, 1693, 2005, 2218, 2596, 2797, 3197, 3245
BseLI	CCNNNNNNNGG	8 cut(s)	109, 372, 1184, 2917, 3150, 3372, 3937, 3938
BseMI	GCAATG	2 cut(s)	489, 3402
BseNI	ACTGG	4 cut(s)	1084, 2167, 2977, 3286
BseRI	GAGGAG	6 cut(s)	22, 1238, 1493, 2397, 2816, 2963
BseSI	GKGCMC	1 cut(s)	3131
BseYI	CCCAGC	3 cut(s)	24, 443, 876
BsgI	GTGCAG	1 cut(s)	369
Bsh1236I	CGCG	6 cut(s)	726, 1313, 1840, 2843, 2863, 3414
BshFI	GGCC	6 cut(s)	2079, 2300, 2633, 2810, 3439, 3612
BshNI	GGYRCC	3 cut(s)	2845, 3295, 3362
BshVI	ATCGAT	3 cut(s)	651, 3336, 3663
BsiHKAI	GWGCWC	8 cut(s)	207, 360, 543, 592, 984, 2194, 3510, 3710
BsiHKCI	CYCGRG	4 cut(s)	407, 3371, 3592, 3709
BsiSI	CCGG	8 cut(s)	124, 210, 250, 279, 313, 369, 2076, 3372
BslFI	GGGAC	3 cut(s)	806, 3482, 3727
BslI	CCNNNNNNNGG	8 cut(s)	109, 372, 1184, 2917, 3150, 3372, 3937, 3938
BsmAI	GTCTC	5 cut(s)	118, 314, 710, 3215, 3647
BsmBI	CGTCTC	1 cut(s)	3215
BsmFI	GGGAC	3 cut(s)	806, 3482, 3727
BsmI	GAATGC	3 cut(s)	1306, 3160, 3808
BsnI	GGCC	6 cut(s)	2079, 2300, 2633, 2810, 3439, 3612
Bso31I	GGTCTC	2 cut(s)	118, 314
BsoBI	CYCGRG	4 cut(s)	407, 3371, 3592, 3709
Bsp119I	TTCGAA	1 cut(s)	1385
Bsp1286I	GDGCHC	9 cut(s)	207, 360, 543, 592, 984, 2194, 3131, 3510, 3710
Bsp13I	TCCGGA	1 cut(s)	249
Bsp143I	GATC	9 cut(s)	733, 1070, 1492, 2196, 2608, 2812, 3333, 3596, 3814
Bsp68I	TCGCGA	1 cut(s)	3414
BspANI	GGCC	6 cut(s)	2079, 2300, 2633, 2810, 3439, 3612
BspDI	ATCGAT	3 cut(s)	651, 3336, 3663
BspEI	TCCGGA	1 cut(s)	249
BspFNI	CGCG	6 cut(s)	726, 1313, 1840, 2843, 2863, 3414
BspHI	TCATGA	1 cut(s)	1516
BspLI	GGNNCC	7 cut(s)	364, 1977, 2294, 2847, 3297, 3364, 3923
BspMAI	CTGCAG	1 cut(s)	2871
BspMI	ACCTGC	1 cut(s)	184
BspPI	GGATC	3 cut(s)	1487, 2616, 3604
BspQI	GCTCTTC	2 cut(s)	1095, 1956
BspT104I	TTCGAA	1 cut(s)	1385
BspT107I	GGYRCC	3 cut(s)	2845, 3295, 3362
BspTI	CTTAAG	2 cut(s)	944, 2260
BspTNI	GGTCTC	2 cut(s)	118, 314
BsrBI	CCGCTC	2 cut(s)	42, 247
BsrDI	GCAATG	2 cut(s)	489, 3402
BsrFI	RCCGGY	2 cut(s)	209, 368
BsrI	ACTGG	4 cut(s)	1084, 2167, 2977, 3286
BssAI	RCCGGY	2 cut(s)	209, 368
BssMI	GATC	9 cut(s)	733, 1070, 1492, 2196, 2608, 2812, 3333, 3596, 3814
BssNAI	GTATAC	1 cut(s)	1052
BssNI	GRCGYC	5 cut(s)	100, 159, 239, 3296, 3741
BssT1I	CCWWGG	1 cut(s)	815
Bst1107I	GTATAC	1 cut(s)	1052
Bst2UI	CCWGG	9 cut(s)	585, 848, 1179, 1994, 2108, 2297, 2879, 2912, 3785
Bst4CI	ACNGT	8 cut(s)	557, 1397, 1413, 1757, 2587, 3451, 3698, 3968
Bst6I	CTCTTC	6 cut(s)	1095, 1256, 1290, 1956, 2168, 2822
BstACI	GRCGYC	5 cut(s)	100, 159, 239, 3296, 3741
BstAFI	CTTAAG	2 cut(s)	944, 2260
BstBI	TTCGAA	1 cut(s)	1385
BstDSI	CCRYGG	1 cut(s)	2586
BstF5I	GGATG	8 cut(s)	1153, 1693, 2005, 2218, 2596, 2797, 3197, 3245
BstFNI	CGCG	6 cut(s)	726, 1313, 1840, 2843, 2863, 3414
BstH2I	RGCGCY	2 cut(s)	31, 3299
BstHHI	GCGC	8 cut(s)	30, 404, 926, 1840, 2843, 2865, 3298, 3389
BstKTI	GATC	9 cut(s)	736, 1073, 1495, 2199, 2611, 2815, 3336, 3599, 3817
BstMAI	GTCTC	5 cut(s)	118, 314, 710, 3215, 3647
BstMBI	GATC	9 cut(s)	733, 1070, 1492, 2196, 2608, 2812, 3333, 3596, 3814
BstNI	CCWGG	9 cut(s)	585, 848, 1179, 1994, 2108, 2297, 2879, 2912, 3785
BstSFI	CTRYAG	1 cut(s)	2867
BstSLI	GKGCMC	1 cut(s)	3131
BstUI	CGCG	6 cut(s)	726, 1313, 1840, 2843, 2863, 3414
BstV2I	GAAGAC	3 cut(s)	178, 820, 996
BstX2I	RGATCY	2 cut(s)	2608, 3596
BstXI	CCANNNNNNTGG	3 cut(s)	2787, 2972, 3580
BstYI	RGATCY	2 cut(s)	2608, 3596
BstZ17I	GTATAC	1 cut(s)	1052
Bsu15I	ATCGAT	3 cut(s)	651, 3336, 3663
BsuI	GTATCC	4 cut(s)	290, 610, 2740, 3636
BsuRI	GGCC	6 cut(s)	2079, 2300, 2633, 2810, 3439, 3612
BsuTUI	ATCGAT	3 cut(s)	651, 3336, 3663
BtgI	CCRYGG	1 cut(s)	2586
BtgZI	GCGATG	3 cut(s)	153, 621, 3170
BtsCI	GGATG	8 cut(s)	1153, 1693, 2005, 2218, 2596, 2797, 3197, 3245
BtsI	GCAGTG	4 cut(s)	1553, 3177, 3267, 3870
BtsIMutI	CAGTG	6 cut(s)	1077, 1553, 3177, 3267, 3835, 3870
BtuMI	TCGCGA	1 cut(s)	3414
BveI	ACCTGC	1 cut(s)	184
CaiI	CAGNNNCTG	3 cut(s)	763, 1079, 3054
CciI	TCATGA	1 cut(s)	1516
CfoI	GCGC	8 cut(s)	30, 404, 926, 1840, 2843, 2865, 3298, 3389
Cfr10I	RCCGGY	2 cut(s)	209, 368
Cfr13I	GGNCC	3 cut(s)	1990, 3437, 3610
Cfr9I	CCCGGG	1 cut(s)	3371
ClaI	ATCGAT	3 cut(s)	651, 3336, 3663
CseI	GACGC	7 cut(s)	108, 167, 247, 734, 803, 1727, 3237
CsiI	ACCWGGT	1 cut(s)	3783
Csp6I	GTAC	9 cut(s)	86, 215, 553, 1857, 2285, 2998, 3005, 3699, 3960
CviQI	GTAC	9 cut(s)	86, 215, 553, 1857, 2285, 2998, 3005, 3699, 3960
DinI	GGCGCC	1 cut(s)	3297
DpnI	GATC	9 cut(s)	735, 1072, 1494, 2198, 2610, 2814, 3335, 3598, 3816
DpnII	GATC	9 cut(s)	733, 1070, 1492, 2196, 2608, 2812, 3333, 3596, 3814
DraI	TTTAAA	1 cut(s)	2578
EaeI	YGGCCR	3 cut(s)	2077, 2298, 2808
Eam1104I	CTCTTC	6 cut(s)	1095, 1256, 1290, 1956, 2168, 2822
EarI	CTCTTC	6 cut(s)	1095, 1256, 1290, 1956, 2168, 2822
EciI	GGCGGA	1 cut(s)	1942
Ecl136II	GAGCTC	5 cut(s)	205, 541, 590, 3508, 3708
Eco130I	CCWWGG	1 cut(s)	815
Eco24I	GRGCYC	5 cut(s)	207, 543, 592, 3510, 3710
Eco31I	GGTCTC	2 cut(s)	118, 314
Eco32I	GATATC	3 cut(s)	1654, 2550, 3087
Eco47I	GGWCC	1 cut(s)	1990
Eco47III	AGCGCT	1 cut(s)	29
Eco53kI	GAGCTC	5 cut(s)	205, 541, 590, 3508, 3708
Eco57I	CTGAAG	3 cut(s)	681, 1237, 1526
Eco88I	CYCGRG	4 cut(s)	407, 3371, 3592, 3709
EcoICRI	GAGCTC	5 cut(s)	205, 541, 590, 3508, 3708
EcoO109I	RGGNCCY	2 cut(s)	1990, 3437
EcoRI	GAATTC	1 cut(s)	637
EcoRII	CCWGG	9 cut(s)	583, 846, 1177, 1992, 2106, 2295, 2877, 2910, 3783
EcoRV	GATATC	3 cut(s)	1654, 2550, 3087
EcoT14I	CCWWGG	1 cut(s)	815
EcoT22I	ATGCAT	2 cut(s)	678, 3810
EcoT38I	GRGCYC	5 cut(s)	207, 543, 592, 3510, 3710
EgeI	GGCGCC	1 cut(s)	3297
EheI	GGCGCC	1 cut(s)	3297
ErhI	CCWWGG	1 cut(s)	815
Esp3I	CGTCTC	1 cut(s)	3215
FalI	AAGNNNNNCTT	4 cut(s)	812, 844, 1235, 1267
FaqI	GGGAC	3 cut(s)	806, 3482, 3727
FauI	CCCGC	2 cut(s)	79, 3939
FauNDI	CATATG	2 cut(s)	678, 1261
FbaI	TGATCA	1 cut(s)	3814
FblI	GTMKAC	2 cut(s)	384, 1051
FokI	GGATG	8 cut(s)	1140, 1700, 2012, 2225, 2603, 2804, 3184, 3252
FriOI	GRGCYC	5 cut(s)	207, 543, 592, 3510, 3710
FspBI	CTAG	8 cut(s)	737, 1019, 1118, 1770, 3356, 3501, 3626, 3737
FspI	TGCGCA	1 cut(s)	3388
GlaI	GCGC	8 cut(s)	29, 403, 925, 1839, 2842, 2864, 3297, 3388
GsaI	CCCAGC	3 cut(s)	28, 447, 880
GsuI	CTGGAG	4 cut(s)	606, 2015, 2894, 2994
HaeII	RGCGCY	2 cut(s)	31, 3299
HaeIII	GGCC	6 cut(s)	2079, 2300, 2633, 2810, 3439, 3612
HapII	CCGG	8 cut(s)	124, 210, 250, 279, 313, 369, 2076, 3372
HgaI	GACGC	7 cut(s)	108, 167, 247, 734, 803, 1727, 3237
HhaI	GCGC	8 cut(s)	30, 404, 926, 1840, 2843, 2865, 3298, 3389
Hin1I	GRCGYC	5 cut(s)	100, 159, 239, 3296, 3741
Hin6I	GCGC	8 cut(s)	28, 402, 924, 1838, 2841, 2863, 3296, 3387
HinP1I	GCGC	8 cut(s)	28, 402, 924, 1838, 2841, 2863, 3296, 3387
HincII	GTYRAC	1 cut(s)	424
HindII	GTYRAC	1 cut(s)	424
HindIII	AAGCTT	3 cut(s)	941, 1538, 2307
HpaII	CCGG	8 cut(s)	124, 210, 250, 279, 313, 369, 2076, 3372
HphI	GGTGA	5 cut(s)	199, 743, 1439, 3187, 3799
Hpy166II	GTNNAC	6 cut(s)	385, 424, 1052, 1393, 1400, 2287
Hpy8I	GTNNAC	6 cut(s)	385, 424, 1052, 1393, 1400, 2287
Hpy99I	CGWCG	4 cut(s)	99, 102, 750, 1721
HpyCH4III	ACNGT	8 cut(s)	557, 1397, 1413, 1757, 2587, 3451, 3698, 3968
HpyCH4IV	ACGT	4 cut(s)	438, 1402, 3125, 3741
HpySE526I	ACGT	4 cut(s)	438, 1402, 3125, 3741
Hsp92I	GRCGYC	5 cut(s)	100, 159, 239, 3296, 3741
HspAI	GCGC	8 cut(s)	28, 402, 924, 1838, 2841, 2863, 3296, 3387
KasI	GGCGCC	1 cut(s)	3295
Kpn2I	TCCGGA	1 cut(s)	249
Ksp22I	TGATCA	1 cut(s)	3814
Kzo9I	GATC	9 cut(s)	733, 1070, 1492, 2196, 2608, 2812, 3333, 3596, 3814
LguI	GCTCTTC	2 cut(s)	1095, 1956
LweI	GCATC	7 cut(s)	1150, 1207, 1678, 1706, 3193, 3206, 3235
MabI	ACCWGGT	1 cut(s)	3783
MaeI	CTAG	8 cut(s)	737, 1019, 1118, 1770, 3356, 3501, 3626, 3737
MaeII	ACGT	4 cut(s)	438, 1402, 3125, 3741
MalI	GATC	9 cut(s)	735, 1072, 1494, 2198, 2610, 2814, 3335, 3598, 3816
MbiI	CCGCTC	2 cut(s)	42, 247
MboI	GATC	9 cut(s)	733, 1070, 1492, 2196, 2608, 2812, 3333, 3596, 3814
MfeI	CAATTG	4 cut(s)	267, 1692, 2348, 3098
MflI	RGATCY	2 cut(s)	2608, 3596
MhlI	GDGCHC	9 cut(s)	207, 360, 543, 592, 984, 2194, 3131, 3510, 3710
Mly113I	GGCGCC	1 cut(s)	3296
MlyI	GAGTC	3 cut(s)	772, 1871, 3464
MmeI	TCCRAC	1 cut(s)	429
Mph1103I	ATGCAT	2 cut(s)	678, 3810
MroI	TCCGGA	1 cut(s)	249
MroXI	GAANNNNTTC	2 cut(s)	2016, 3139
MslI	CAYNNNNRTG	2 cut(s)	1158, 2785
MspA1I	CMGCKG	3 cut(s)	353, 1442, 2459
MspCI	CTTAAG	2 cut(s)	944, 2260
MspI	CCGG	8 cut(s)	124, 210, 250, 279, 313, 369, 2076, 3372
MunI	CAATTG	4 cut(s)	267, 1692, 2348, 3098
Mva1269I	GAATGC	3 cut(s)	1306, 3160, 3808
MvaI	CCWGG	9 cut(s)	585, 848, 1179, 1994, 2108, 2297, 2879, 2912, 3785
MvnI	CGCG	6 cut(s)	726, 1313, 1840, 2843, 2863, 3414
NarI	GGCGCC	1 cut(s)	3296
NciI	CCSGG	4 cut(s)	124, 2076, 3372, 3373
NdeI	CATATG	2 cut(s)	678, 1261
NdeII	GATC	9 cut(s)	733, 1070, 1492, 2196, 2608, 2812, 3333, 3596, 3814
NlaIV	GGNNCC	7 cut(s)	364, 1977, 2294, 2847, 3297, 3364, 3923
NmeAIII	GCCGAG	3 cut(s)	242, 562, 2326
NmuCI	GTSAC	7 cut(s)	256, 565, 866, 1909, 2694, 2756, 3742
NruI	TCGCGA	1 cut(s)	3414
NsbI	TGCGCA	1 cut(s)	3388
NsiI	ATGCAT	2 cut(s)	678, 3810
NspV	TTCGAA	1 cut(s)	1385
PagI	TCATGA	1 cut(s)	1516
PaqCI	CACCTGC	1 cut(s)	184
PciSI	GCTCTTC	2 cut(s)	1095, 1956
PctI	GAATGC	3 cut(s)	1306, 3160, 3808
PdmI	GAANNNNTTC	2 cut(s)	2016, 3139
PflMI	CCANNNNNTGG	1 cut(s)	2917
PfoI	TCCNGGA	4 cut(s)	122, 583, 1992, 2910
PleI	GAGTC	3 cut(s)	772, 1870, 3464
PluTI	GGCGCC	1 cut(s)	3299
PpsI	GAGTC	3 cut(s)	772, 1870, 3464
PpuMI	RGGWCCY	1 cut(s)	1990
PshBI	ATTAAT	1 cut(s)	2619
PsiI	TTATAA	1 cut(s)	3777
Psp124BI	GAGCTC	5 cut(s)	207, 543, 592, 3510, 3710
Psp5II	RGGWCCY	1 cut(s)	1990
Psp6I	CCWGG	9 cut(s)	583, 846, 1177, 1992, 2106, 2295, 2877, 2910, 3783
PspFI	CCCAGC	3 cut(s)	24, 443, 876
PspGI	CCWGG	9 cut(s)	583, 846, 1177, 1992, 2106, 2295, 2877, 2910, 3783
PspN4I	GGNNCC	7 cut(s)	364, 1977, 2294, 2847, 3297, 3364, 3923
PspPI	GGNCC	3 cut(s)	1990, 3437, 3610
PspPPI	RGGWCCY	1 cut(s)	1990
PstI	CTGCAG	1 cut(s)	2871
PstNI	CAGNNNCTG	3 cut(s)	763, 1079, 3054
PsuI	RGATCY	2 cut(s)	2608, 3596
PvuII	CAGCTG	1 cut(s)	2459
RruI	TCGCGA	1 cut(s)	3414
RsaI	GTAC	9 cut(s)	87, 216, 554, 1858, 2286, 2999, 3006, 3700, 3961
RsaNI	GTAC	9 cut(s)	86, 215, 553, 1857, 2285, 2998, 3005, 3699, 3960
RseI	CAYNNNNRTG	2 cut(s)	1158, 2785
SacI	GAGCTC	5 cut(s)	207, 543, 592, 3510, 3710
SapI	GCTCTTC	2 cut(s)	1095, 1956
Sau3AI	GATC	9 cut(s)	733, 1070, 1492, 2196, 2608, 2812, 3333, 3596, 3814
Sau96I	GGNCC	3 cut(s)	1990, 3437, 3610
SchI	GAGTC	3 cut(s)	772, 1871, 3464
SduI	GDGCHC	9 cut(s)	207, 360, 543, 592, 984, 2194, 3131, 3510, 3710
SexAI	ACCWGGT	1 cut(s)	3783
SfaNI	GCATC	7 cut(s)	1150, 1207, 1678, 1706, 3193, 3206, 3235
SfcI	CTRYAG	1 cut(s)	2867
SfoI	GGCGCC	1 cut(s)	3297
SfuI	TTCGAA	1 cut(s)	1385
SgrAI	CRCCGGYG	2 cut(s)	209, 368
SinI	GGWCC	1 cut(s)	1990
SmaI	CCCGGG	1 cut(s)	3373
SmiMI	CAYNNNNRTG	2 cut(s)	1158, 2785
SmlI	CTYRAG	4 cut(s)	591, 713, 944, 2260
SmoI	CTYRAG	4 cut(s)	591, 713, 944, 2260
SpeI	ACTAGT	2 cut(s)	1018, 3500
SspDI	GGCGCC	1 cut(s)	3295
SspI	AATATT	2 cut(s)	2497, 2617
SspMI	CTAG	8 cut(s)	737, 1019, 1118, 1770, 3356, 3501, 3626, 3737
SstI	GAGCTC	5 cut(s)	207, 543, 592, 3510, 3710
StyI	CCWWGG	1 cut(s)	815
TaaI	ACNGT	8 cut(s)	557, 1397, 1413, 1757, 2587, 3451, 3698, 3968
TaiI	ACGT	4 cut(s)	441, 1405, 3128, 3744
TatI	WGTACW	4 cut(s)	552, 2284, 2997, 3698
TauI	GCSGC	8 cut(s)	77, 137, 164, 244, 247, 368, 419, 2846
TscAI	CASTG	6 cut(s)	1084, 1553, 3177, 3274, 3835, 3877
TseFI	GTSAC	7 cut(s)	256, 565, 866, 1909, 2694, 2756, 3742
Tsp45I	GTSAC	7 cut(s)	256, 565, 866, 1909, 2694, 2756, 3742
TspGWI	ACGGA	6 cut(s)	73, 854, 2172, 2285, 3197, 3655
TspMI	CCCGGG	1 cut(s)	3371
TspRI	CASTG	6 cut(s)	1084, 1553, 3177, 3274, 3835, 3877
Van91I	CCANNNNNTGG	1 cut(s)	2917
Vha464I	CTTAAG	2 cut(s)	944, 2260
VpaK11BI	GGWCC	1 cut(s)	1990
VspI	ATTAAT	1 cut(s)	2619
XapI	RAATTY	3 cut(s)	637, 696, 3274
XbaI	TCTAGA	2 cut(s)	736, 1769
XcmI	CCANNNNNNNNNTGG	2 cut(s)	1288, 3913
XmaI	CCCGGG	1 cut(s)	3371
XmiI	GTMKAC	2 cut(s)	384, 1051
XmnI	GAANNNNTTC	2 cut(s)	2016, 3139
XspI	CTAG	8 cut(s)	737, 1019, 1118, 1770, 3356, 3501, 3626, 3737
ZraI	GACGTC	1 cut(s)	3742
Zsp2I	ATGCAT	2 cut(s)	678, 3810

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.