Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr03

Orthogroup ID

OG0013869

Physical Location & Seq

Genomic Coordinates Forward (+)

4813144 .. 4815758

Gene Structure 2615 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD03G1059600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1389 bp

ATGGAGAATAAATCGCATGTGCTGATGCAAAGATATGAGTTGGGGAGATTGTTGGGTCAAGGGACTTTTGCCAGGGTTCACTATGCAAGGAGCTTGATAACGAATCAGGGTGTGGCCATTAAGGTTATTGACAAAGAAAAGATTATGAAGGTCGGGTTGATGGATCAGATCAAGCGAGAAATATCTGTTATGAGACTGGTTAGACACCCTAATATTATACACCTCTATGAAGTCATGGCAACCAAAACTAAGATATACTTTGTCATTGAATATGCTAAAGGCGGTGAGCTATTTAACAAGGTTGCTAAAGGAAAGCTGAAGGAGGACGTTGCAAGGAAATATTTTTCGCAGCTAATTGATGCTCTTGATTTCTGTCATAGTAGGGGTGTTTATCATCGGGATATAAAACCAGAGAACTTGTTGCTAGATGAGAATGATAATTTGAAGATCTCTGATTTTGGGTTAAGTGCCCTCGCTGAAAGCAAGCGCCAAGATGGGCTACTCCACACCACCTGTGGTACTCCTGCCTATGTTGCTCCGGAAGTTATTAACAGGAGAGGCTACGATGGTGTGAAAGCTGATATTTGGTCTTGTGGAGTTGTCTTGTATGTCTTATTGGCAGGCTGTCTACCATTTCAAGATTCAAATTTGATGGAGATGTACCGGAAGATTGGGAAAGCAGAATTTAAATGCCCAAATTTTTTCTCACCGGAAGCACGTAGGCTACTGTACAGGATGTTGGATCCAAATCCCAATTCTAGGATTTCCATAGCCAAAGTTGGGGAAAGTTCTTGGTACAGAAAGGGACCATACTCCAAAAATATGAAATCTGAAATAGGAAACAAGGATGCGGCTCGTATGAGTGCAGAAGCTTCCGGTTCCAGTGAGAATAGTATGGCTTCTGAGGAAAAGCAAGAACCAGGGAGACCTCCAAACATGAATGCTTTTGATATTATCTCCCTTTCTGCCGGATTTGATCTGTCTGGCTTGTTTGAAAAAGATCCTTTTCACAGAGTAGTAAGATTCACTTCCAGACAACCTGCTACGGTCATCATCTCCAAGTTAGAAGAAATGGCTAAGCAACTGGAGCTGAAAGTGAAGAAGAAGGATGATGGATTGTTGAAAATTGATGGATTAAAGGAAGGTAGAAAGGGGATTTTGTCCATAGATGCAGAGATATTTGAAATCACTCCAACTTTTCATCTGGTCGAGGTGAAGAAATCAAGTGGGGATACATTGGAATTCCAGAAGATGTTGGACGACATAAGACCTGCTCTTCGAGACATAGTCTGGGTTTGGCAAGGTGAGGAAGAGCAGCCACAGCAACAACAACCAGAAATCCCGCCGCAGCAACAAGAACAACTGCCTCAGCATCCTCCACGACAGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005575 GO:0005622 GO:0005623 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0009987 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0018105 GO:0018107 GO:0018193 GO:0018209 GO:0018210 GO:0019538 GO:0023052 GO:0035556 GO:0036211 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044464 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0065007 GO:0071704 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

ko04068 ko04113 ko04138 ko04140 ko04150 ko04151 ko04152 ko04211 ko04213 ko04371 ko04530 ko04710 ko04714 ko04910 ko04920 ko04921 ko04922 ko04931 ko04932 ko05410 ko05418 map04068 map04113 map04138 map04140 map04150 map04151 map04152 map04211 map04213 map04371 map04530 map04710 map04714 map04910 map04920 map04921 map04922 map04931 map04932 map05410 map05418

Pfam Domains

Protein Families

NAF Pkinase

Protein Analysis

463

Amino Acids

52.57

Weight (kDa)

8.79

Isoelectric Point (pI)

45.03

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Pkinase	PF00069	12 - 265	2.5e-74	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	13 - 260	1.2e-38	Protein tyrosine and serine/threonine kinase
Kinase-like	PF14531	104 - 254	1.2e-06	Kinase-like
NAF	PF03822	310 - 368	3e-24	NAF domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0013869)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G07070 AT5G58380
fragaria_vesca	FvH4_3g40220 FvH4_3g40220
malus_domestica	MD03G1059600.v1.1 MD11G1061700.v1.1
prunus_persica	Prupe.6G043700_v2.0.a1
rosa_chinensis	RchiOBHm_Chr5g0072431
rosa_laevigata	RLG00000036298
rosa_multiflora	Rmu_sc0008676.1_g000003
rosa_roxburghii	Rroxscaffold_1G00008780
rosa_rugosa	Rorug05G0418100 Rorug05G0418200
rosa_samantha	Rh5AG474600 Rh5BG494700 Rh5CG518700
rosa_wichuraiana	Rw5G044070

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	2 cut(s)	1048, 1279
AccI	GTMKAC	1 cut(s)	628
AccIII	TCCGGA	1 cut(s)	538
AciI	CCGC	4 cut(s)	282, 851, 1343, 1346
AclWI	GGATC	4 cut(s)	171, 737, 750, 995
AcoI	YGGCCR	1 cut(s)	114
AcsI	RAATTY	4 cut(s)	646, 683, 697, 1241
AcuI	CTGAAG	1 cut(s)	338
AfaI	GTAC	4 cut(s)	520, 662, 731, 797
AfiI	CCNNNNNNNGG	2 cut(s)	759, 780
AgsI	TTSAA	7 cut(s)	269, 445, 638, 645, 995, 1123, 1184
AjnI	CCWGG	2 cut(s)	71, 919
AjuI	GAANNNNNNNTTGG	2 cut(s)	739, 771
AluBI	AGCT	7 cut(s)	93, 289, 316, 352, 578, 872, 1090
AluI	AGCT	7 cut(s)	93, 289, 316, 352, 578, 872, 1090
Alw26I	GTCTC	3 cut(s)	187, 919, 1275
AlwI	GGATC	4 cut(s)	171, 737, 750, 995
Aor13HI	TCCGGA	1 cut(s)	538
AoxI	GGCC	1 cut(s)	114
ApeKI	GCWGC	3 cut(s)	349, 1315, 1348
ApoI	RAATTY	4 cut(s)	646, 683, 697, 1241
ArsI	GACNNNNNNTTYG	2 cut(s)	1260, 1292
AspLEI	GCGC	1 cut(s)	489
AspS9I	GGNCC	1 cut(s)	806
AsuHPI	GGTGA	4 cut(s)	296, 699, 1225, 1316
AvaII	GGWCC	1 cut(s)	806
BaeGI	GKGCMC	1 cut(s)	472
BalI	TGGCCA	1 cut(s)	116
BamHI	GGATCC	1 cut(s)	742
BbvCI	CCTCAGC	1 cut(s)	1368
BbvI	GCAGC	3 cut(s)	361, 1327, 1360
BccI	CCATC	6 cut(s)	154, 488, 560, 646, 1106, 1124
BciT130I	CCWGG	2 cut(s)	73, 921
BciVI	GTATCC	1 cut(s)	1225
BcoDI	GTCTC	3 cut(s)	187, 919, 1275
BfaI	CTAG	2 cut(s)	425, 759
BfoI	RGCGCY	1 cut(s)	490
BfuAI	ACCTGC	2 cut(s)	1048, 1279
BfuI	GTATCC	1 cut(s)	1225
BglII	AGATCT	1 cut(s)	447
BisI	GCNGC	5 cut(s)	350, 852, 1316, 1346, 1349
BlpI	GCTNAGC	1 cut(s)	1077
BlsI	GCNGC	5 cut(s)	351, 853, 1317, 1347, 1350
Bme1390I	CCNGG	2 cut(s)	73, 921
Bme18I	GGWCC	1 cut(s)	806
BmgT120I	GGNCC	1 cut(s)	806
BmiI	GGNNCC	3 cut(s)	744, 807, 880
BmrFI	CCNGG	2 cut(s)	73, 921
BmsI	GCATC	5 cut(s)	15, 349, 838, 1159, 1381
BpmI	CTGGAG	1 cut(s)	1106
Bpu10I	CCTNAGC	1 cut(s)	1368
Bpu1102I	GCTNAGC	1 cut(s)	1077
BsaAI	YACGTR	1 cut(s)	719
BsaBI	GATNNNNATC	1 cut(s)	747
BsaI	GGTCTC	1 cut(s)	919
BsaJI	CCNNGG	2 cut(s)	72, 920
BsaWI	WCCGGW	4 cut(s)	538, 663, 709, 875
Bsc4I	CCNNNNNNNGG	2 cut(s)	759, 780
Bse1I	ACTGG	3 cut(s)	201, 882, 1089
Bse8I	GATNNNNATC	1 cut(s)	747
BseAI	TCCGGA	1 cut(s)	538
BseBI	CCWGG	2 cut(s)	73, 921
BseDI	CCNNGG	2 cut(s)	72, 920
BseGI	GGATG	4 cut(s)	741, 853, 1114, 1372
BseJI	GATNNNNATC	1 cut(s)	747
BseLI	CCNNNNNNNGG	2 cut(s)	759, 780
BseMII	CTCAG	2 cut(s)	894, 1382
BseNI	ACTGG	3 cut(s)	201, 882, 1089
BseSI	GKGCMC	1 cut(s)	472
BseXI	GCAGC	3 cut(s)	361, 1327, 1360
BsgI	GTGCAG	1 cut(s)	885
BshFI	GGCC	1 cut(s)	116
BsiSI	CCGG	5 cut(s)	539, 664, 710, 876, 969
BslFI	GGGAC	2 cut(s)	76, 819
BslI	CCNNNNNNNGG	2 cut(s)	759, 780
BsmAI	GTCTC	3 cut(s)	187, 919, 1275
BsmFI	GGGAC	2 cut(s)	76, 819
BsmI	GAATGC	1 cut(s)	946
BsnI	GGCC	1 cut(s)	116
Bso31I	GGTCTC	1 cut(s)	919
Bsp1286I	GDGCHC	1 cut(s)	472
Bsp13I	TCCGGA	1 cut(s)	538
Bsp1407I	TGTACA	1 cut(s)	729
Bsp143I	GATC	6 cut(s)	163, 168, 447, 742, 976, 1000
Bsp1720I	GCTNAGC	1 cut(s)	1077
BspACI	CCGC	4 cut(s)	282, 851, 1343, 1346
BspANI	GGCC	1 cut(s)	116
BspCNI	CTCAG	2 cut(s)	895, 1381
BspEI	TCCGGA	1 cut(s)	538
BspLI	GGNNCC	3 cut(s)	744, 807, 880
BspMI	ACCTGC	2 cut(s)	1048, 1279
BspPI	GGATC	4 cut(s)	171, 737, 750, 995
BspQI	GCTCTTC	2 cut(s)	1281, 1305
BspTNI	GGTCTC	1 cut(s)	919
BsrGI	TGTACA	1 cut(s)	729
BsrI	ACTGG	3 cut(s)	201, 882, 1089
BssECI	CCNNGG	2 cut(s)	72, 920
BssMI	GATC	6 cut(s)	163, 168, 447, 742, 976, 1000
Bst2UI	CCWGG	2 cut(s)	73, 921
Bst4CI	ACNGT	3 cut(s)	729, 1048, 1386
Bst6I	CTCTTC	2 cut(s)	1281, 1305
BstAUI	TGTACA	1 cut(s)	729
BstBAI	YACGTR	1 cut(s)	719
BstC8I	GCNNGC	2 cut(s)	485, 622
BstDEI	CTNAG	4 cut(s)	249, 903, 1077, 1368
BstF5I	GGATG	4 cut(s)	741, 853, 1114, 1372
BstH2I	RGCGCY	1 cut(s)	490
BstHHI	GCGC	1 cut(s)	489
BstKTI	GATC	6 cut(s)	166, 171, 450, 745, 979, 1003
BstMAI	GTCTC	3 cut(s)	187, 919, 1275
BstMBI	GATC	6 cut(s)	163, 168, 447, 742, 976, 1000
BstMWI	GCNNNNNNNGC	3 cut(s)	533, 1087, 1321
BstNI	CCWGG	2 cut(s)	73, 921
BstNSI	RCATGY	1 cut(s)	20
BstSCI	CCNGG	2 cut(s)	71, 919
BstSLI	GKGCMC	1 cut(s)	472
BstV1I	GCAGC	3 cut(s)	361, 1327, 1360
BstX2I	RGATCY	3 cut(s)	447, 742, 1000
BstYI	RGATCY	3 cut(s)	447, 742, 1000
BsuI	GTATCC	1 cut(s)	1225
BsuRI	GGCC	1 cut(s)	116
BtsCI	GGATG	4 cut(s)	741, 853, 1114, 1372
BtsIMutI	CAGTG	1 cut(s)	889
BveI	ACCTGC	2 cut(s)	1048, 1279
Cac8I	GCNNGC	2 cut(s)	485, 622
CfoI	GCGC	1 cut(s)	489
Cfr13I	GGNCC	1 cut(s)	806
Csp6I	GTAC	4 cut(s)	519, 661, 730, 796
CviAII	CATG	3 cut(s)	17, 235, 937
CviQI	GTAC	4 cut(s)	519, 661, 730, 796
DdeI	CTNAG	4 cut(s)	249, 903, 1077, 1368
DpnI	GATC	6 cut(s)	165, 170, 449, 744, 978, 1002
DpnII	GATC	6 cut(s)	163, 168, 447, 742, 976, 1000
DraI	TTTAAA	1 cut(s)	688
EaeI	YGGCCR	1 cut(s)	114
Eam1104I	CTCTTC	2 cut(s)	1281, 1305
EarI	CTCTTC	2 cut(s)	1281, 1305
Eco31I	GGTCTC	1 cut(s)	919
Eco47I	GGWCC	1 cut(s)	806
Eco57I	CTGAAG	1 cut(s)	338
EcoRI	GAATTC	1 cut(s)	1241
EcoRII	CCWGG	2 cut(s)	71, 919
FaeI	CATG	3 cut(s)	20, 238, 940
FalI	AAGNNNNNCTT	4 cut(s)	242, 274, 1012, 1044
FaqI	GGGAC	2 cut(s)	76, 819
FatI	CATG	3 cut(s)	16, 234, 936
FauI	CCCGC	1 cut(s)	1350
FblI	GTMKAC	1 cut(s)	628
Fnu4HI	GCNGC	5 cut(s)	350, 852, 1316, 1346, 1349
FokI	GGATG	4 cut(s)	748, 860, 1121, 1359
Fsp4HI	GCNGC	5 cut(s)	350, 852, 1316, 1346, 1349
FspBI	CTAG	2 cut(s)	425, 759
GlaI	GCGC	1 cut(s)	488
GluI	GCNGC	5 cut(s)	350, 852, 1316, 1346, 1349
GsuI	CTGGAG	1 cut(s)	1106
HaeII	RGCGCY	1 cut(s)	490
HaeIII	GGCC	1 cut(s)	116
HapII	CCGG	5 cut(s)	539, 664, 710, 876, 969
HhaI	GCGC	1 cut(s)	489
Hin1II	CATG	3 cut(s)	20, 238, 940
Hin6I	GCGC	1 cut(s)	487
HinP1I	GCGC	1 cut(s)	487
HindIII	AAGCTT	1 cut(s)	870
HinfI	GANTC	3 cut(s)	103, 641, 1023
HpaII	CCGG	5 cut(s)	539, 664, 710, 876, 969
HphI	GGTGA	4 cut(s)	296, 699, 1225, 1316
Hpy166II	GTNNAC	2 cut(s)	79, 629
Hpy188I	TCNGA	4 cut(s)	168, 454, 832, 904
Hpy188III	TCNNGA	7 cut(s)	365, 398, 539, 638, 1032, 1246, 1280
Hpy8I	GTNNAC	2 cut(s)	79, 629
HpyAV	CCTTC	4 cut(s)	142, 313, 1099, 1136
HpyCH4III	ACNGT	3 cut(s)	729, 1048, 1386
HpyCH4IV	ACGT	2 cut(s)	327, 718
HpyCH4V	TGCA	5 cut(s)	28, 86, 332, 866, 1172
HpyF10VI	GCNNNNNNNGC	3 cut(s)	533, 1087, 1321
HpyF3I	CTNAG	4 cut(s)	249, 903, 1077, 1368
HpySE526I	ACGT	2 cut(s)	327, 718
Hsp92II	CATG	3 cut(s)	20, 238, 940
HspAI	GCGC	1 cut(s)	487
Kpn2I	TCCGGA	1 cut(s)	538
Kzo9I	GATC	6 cut(s)	163, 168, 447, 742, 976, 1000
LguI	GCTCTTC	2 cut(s)	1281, 1305
LmnI	GCTCC	3 cut(s)	90, 541, 1087
Lsp1109I	GCAGC	3 cut(s)	361, 1327, 1360
LweI	GCATC	5 cut(s)	15, 349, 838, 1159, 1381
MaeI	CTAG	2 cut(s)	425, 759
MaeII	ACGT	2 cut(s)	327, 718
MalI	GATC	6 cut(s)	165, 170, 449, 744, 978, 1002
MboI	GATC	6 cut(s)	163, 168, 447, 742, 976, 1000
MboII	GAAGA	9 cut(s)	457, 679, 1079, 1111, 1114, 1228, 1261, 1268, 1322
MflI	RGATCY	3 cut(s)	447, 742, 1000
MhlI	GDGCHC	1 cut(s)	472
MlsI	TGGCCA	1 cut(s)	116
MluCI	AATT	8 cut(s)	354, 439, 646, 683, 697, 754, 1125, 1241
MluNI	TGGCCA	1 cut(s)	116
MmeI	TCCRAC	3 cut(s)	720, 1217, 1236
Mox20I	TGGCCA	1 cut(s)	116
MroI	TCCGGA	1 cut(s)	538
MscI	TGGCCA	1 cut(s)	116
MseI	TTAA	6 cut(s)	120, 294, 464, 549, 687, 1136
MslI	CAYNNNNRTG	1 cut(s)	225
Msp20I	TGGCCA	1 cut(s)	116
MspI	CCGG	5 cut(s)	539, 664, 710, 876, 969
MspR9I	CCNGG	2 cut(s)	73, 921
Mva1269I	GAATGC	1 cut(s)	946
MvaI	CCWGG	2 cut(s)	73, 921
MwoI	GCNNNNNNNGC	3 cut(s)	533, 1087, 1321
NdeII	GATC	6 cut(s)	163, 168, 447, 742, 976, 1000
NlaIII	CATG	3 cut(s)	20, 238, 940
NlaIV	GGNNCC	3 cut(s)	744, 807, 880
NspI	RCATGY	1 cut(s)	20
PciSI	GCTCTTC	2 cut(s)	1281, 1305
PctI	GAATGC	1 cut(s)	946
PfeI	GAWTC	3 cut(s)	103, 641, 1023
PflFI	GACNNNGTC	1 cut(s)	1286
PkrI	GCNGC	5 cut(s)	351, 853, 1317, 1347, 1350
Ppu21I	YACGTR	1 cut(s)	719
Psp6I	CCWGG	2 cut(s)	71, 919
PspGI	CCWGG	2 cut(s)	71, 919
PspN4I	GGNNCC	3 cut(s)	744, 807, 880
PspPI	GGNCC	1 cut(s)	806
PsuI	RGATCY	3 cut(s)	447, 742, 1000
PsyI	GACNNNGTC	1 cut(s)	1286
RsaI	GTAC	4 cut(s)	520, 662, 731, 797
RsaNI	GTAC	4 cut(s)	519, 661, 730, 796
RseI	CAYNNNNRTG	1 cut(s)	225
SapI	GCTCTTC	2 cut(s)	1281, 1305
SaqAI	TTAA	6 cut(s)	120, 294, 464, 549, 687, 1136
SatI	GCNGC	5 cut(s)	350, 852, 1316, 1346, 1349
Sau3AI	GATC	6 cut(s)	163, 168, 447, 742, 976, 1000
Sau96I	GGNCC	1 cut(s)	806
ScrFI	CCNGG	2 cut(s)	73, 921
SduI	GDGCHC	1 cut(s)	472
SfaNI	GCATC	5 cut(s)	15, 349, 838, 1159, 1381
SinI	GGWCC	1 cut(s)	806
SmiI	ATTTAAAT	1 cut(s)	688
SmiMI	CAYNNNNRTG	1 cut(s)	225
Sse9I	AATT	8 cut(s)	354, 439, 646, 683, 697, 754, 1125, 1241
SsiI	CCGC	4 cut(s)	282, 851, 1343, 1346
SspI	AATATT	2 cut(s)	214, 341
SspMI	CTAG	2 cut(s)	425, 759
StyD4I	CCNGG	2 cut(s)	71, 919
SwaI	ATTTAAAT	1 cut(s)	688
TaaI	ACNGT	3 cut(s)	729, 1048, 1386
TaiI	ACGT	2 cut(s)	330, 721
TaqI	TCGA	2 cut(s)	1209, 1279
TasI	AATT	8 cut(s)	354, 439, 646, 683, 697, 754, 1125, 1241
TatI	WGTACW	1 cut(s)	729
TauI	GCSGC	2 cut(s)	854, 1348
TfiI	GAWTC	3 cut(s)	103, 641, 1023
Tru1I	TTAA	6 cut(s)	120, 294, 464, 549, 687, 1136
Tru9I	TTAA	6 cut(s)	120, 294, 464, 549, 687, 1136
TscAI	CASTG	1 cut(s)	889
TseI	GCWGC	3 cut(s)	349, 1315, 1348
TspDTI	ATGAA	5 cut(s)	161, 243, 839, 953, 1190
TspRI	CASTG	1 cut(s)	889
Tth111I	GACNNNGTC	1 cut(s)	1286
VpaK11BI	GGWCC	1 cut(s)	806
XapI	RAATTY	4 cut(s)	646, 683, 697, 1241
XceI	RCATGY	1 cut(s)	20
XcmI	CCANNNNNNNNNTGG	1 cut(s)	512
XmiI	GTMKAC	1 cut(s)	628
XspI	CTAG	2 cut(s)	425, 759

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.