Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr03

Orthogroup ID

OG0016809

Physical Location & Seq

Genomic Coordinates Reverse (-)

5163463 .. 5164281

Gene Structure 819 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD03G1064500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 819 bp

ATGTTCAGTCCCAATAACTCCACCACCAACCCCCTCAAACAACTCGTCGACGCCATCTTTTCATACAACGGCAATGTCATGCTAGCCGCCGTGGTCTCTCTTCTTCTCGTCATTCTCTTCGTCCTCGTCCTCCACATCTACGCCAAGTGGTTCTTAGCTCAAGCCCACCACCGCCGCCGGGGCAGCTCCGTCACCGTATCTCATGTCCTCGGCCCGAACCGCTTCCAGAACTTCCACACCTTCTCATTGGACGCAAACTCGGCCACCATTAGCAATCTTTCGAGCTCTGCTTCATCCAAAGGCCTAGACTCGAAAACCATCTCCGCCATTCCTCTATTCGTGTACAAAACAGAGGAGCTGGAGGAGATGGTGGAGGAGGAAAATTACAAAAATAGGGTTTTGGAGATGGAGTGTGTAATTTGTTTGAGCCTGTTCGAGGACAACGACATGGGAAGGAGTTTGCCGAAATGTGGTCACTGTTTCCATGTCGAGTGTATCGATATGTGGCTGGGTTCTCACACGAACTGCCCAATTTGCCGAGGTCCGATAGCGGTTGATACGACGGCGGAACGGGTTGCCGGTGAAGAGGAATCGGTTGTGATTGATGTTTTGGATTCTGGGTATGTTTCAAATTGTGATATTGTTCATGTTGGAGCGATGGGTACTGAAACCGAATCGACACCTTCATCGTCTTCGTCTTCCTCGTCATCATCGCCATTAATGGTGGGGTATTCATTGAAGAGAATGCTGAGCAGAAATAGGCCGGAAAGTGGCAAGGTTTTCCCAACTACAAATGGGAGTGATCAAACAGATGCCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004842 GO:0006464 GO:0006508 GO:0006511 GO:0006807 GO:0006950 GO:0006952 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009987 GO:0010033 GO:0010200 GO:0010243 GO:0010498 GO:0016567 GO:0016740 GO:0019538 GO:0019787 GO:0019941 GO:0030163 GO:0032446 GO:0036211 GO:0042221 GO:0042493 GO:0043161 GO:0043170 GO:0043412 GO:0043632 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0050896 GO:0051603 GO:0061630 GO:0061659 GO:0070647 GO:0071704 GO:0140096 GO:1901564 GO:1901565 GO:1901575 GO:1901698 GO:1901700

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

zf-RING_2

Protein Analysis

273

Amino Acids

29.73

Weight (kDa)

5.33

Isoelectric Point (pI)

45.86

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
zf-rbx1	PF12678	135 - 180	4.1e-08	RING-H2 zinc finger domain
zf-RING_2	PF13639	137 - 180	1.2e-12	Ring finger domain
zf-RING_11	PF17123	137 - 166	2.6e-08	RING-like zinc finger
zf-C3HC4_2	PF13923	137 - 179	3e-07	Zinc finger, C3HC4 type (RING finger)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0016809)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_3g39090
malus_domestica	MD03G1064500.v1.1 MD11G1068600.v1.1
prunus_persica	Prupe.6G053700_v2.0.a1
pyrus_communis	pycom03g05180
rosa_chinensis	RchiOBHm_Chr5g0070411
rosa_laevigata	RLG00000036131
rosa_multiflora	Rmu_sc0007961.1_g000012
rosa_roxburghii	Rroxscaffold_1G00010790
rosa_rugosa	Rorug05G0405800
rosa_samantha	Rh5BG479500 Rh5CG503700 Rh5DG491500
rosa_wichuraiana	Rw5G042990

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccI	GTMKAC	1 cut(s)	48
AciI	CCGC	7 cut(s)	87, 172, 175, 220, 324, 551, 566
AcoI	YGGCCR	1 cut(s)	261
AcyI	GRCGYC	1 cut(s)	51
AfaI	GTAC	2 cut(s)	344, 664
AfiI	CCNNNNNNNGG	4 cut(s)	178, 436, 470, 770
AgsI	TTSAA	2 cut(s)	630, 739
AluBI	AGCT	4 cut(s)	158, 186, 285, 358
AluI	AGCT	4 cut(s)	158, 186, 285, 358
Alw21I	GWGCWC	1 cut(s)	287
Alw26I	GTCTC	1 cut(s)	100
AoxI	GGCC	4 cut(s)	211, 261, 301, 761
ApeKI	GCWGC	1 cut(s)	183
ArsI	GACNNNNNNTTYG	2 cut(s)	30, 62
AseI	ATTAAT	1 cut(s)	719
Asp700I	GAANNNNTTC	1 cut(s)	221
AspS9I	GGNCC	2 cut(s)	212, 542
AsuC2I	CCSGG	1 cut(s)	179
AsuHPI	GGTGA	2 cut(s)	184, 593
AsuNHI	GCTAGC	1 cut(s)	82
AvaII	GGWCC	1 cut(s)	542
BanII	GRGCYC	1 cut(s)	287
BbsI	GAAGAC	2 cut(s)	684, 690
Bbv12I	GWGCWC	1 cut(s)	287
BbvI	GCAGC	1 cut(s)	195
BccI	CCATC	5 cut(s)	62, 326, 361, 400, 652
BceAI	ACGGC	3 cut(s)	74, 85, 579
BclI	TGATCA	1 cut(s)	802
BcnI	CCSGG	1 cut(s)	179
BcoDI	GTCTC	1 cut(s)	100
BfaI	CTAG	2 cut(s)	83, 305
BglI	GCCNNNNNGGC	1 cut(s)	180
BisI	GCNGC	3 cut(s)	87, 175, 184
BlpI	GCTNAGC	1 cut(s)	749
BlsI	GCNGC	3 cut(s)	88, 176, 185
Bme1390I	CCNGG	1 cut(s)	179
Bme18I	GGWCC	1 cut(s)	542
BmgT120I	GGNCC	2 cut(s)	212, 542
BmrFI	CCNGG	1 cut(s)	179
BmsI	GCATC	1 cut(s)	802
BmtI	GCTAGC	1 cut(s)	86
BpiI	GAAGAC	2 cut(s)	684, 690
BpmI	CTGGAG	1 cut(s)	380
Bpu1102I	GCTNAGC	1 cut(s)	749
BpuEI	CTTGAG	1 cut(s)	144
BpuMI	CCSGG	1 cut(s)	179
Bsa29I	ATCGAT	1 cut(s)	498
BsaHI	GRCGYC	1 cut(s)	51
BsaI	GGTCTC	1 cut(s)	100
BsaJI	CCNNGG	4 cut(s)	90, 178, 208, 538
BsaXI	ACNNNNNCTCC	2 cut(s)	395, 425
Bsc4I	CCNNNNNNNGG	4 cut(s)	178, 436, 470, 770
Bse118I	RCCGGY	1 cut(s)	578
Bse3DI	GCAATG	1 cut(s)	79
BseCI	ATCGAT	1 cut(s)	498
BseDI	CCNNGG	4 cut(s)	90, 178, 208, 538
BseGI	GGATG	1 cut(s)	293
BseLI	CCNNNNNNNGG	4 cut(s)	178, 436, 470, 770
BseMI	GCAATG	1 cut(s)	79
BseMII	CTCAG	1 cut(s)	740
BseRI	GAGGAG	3 cut(s)	368, 377, 389
BseXI	GCAGC	1 cut(s)	195
BseYI	CCCAGC	1 cut(s)	508
BshFI	GGCC	4 cut(s)	213, 263, 303, 763
BshVI	ATCGAT	1 cut(s)	498
BsiHKAI	GWGCWC	1 cut(s)	287
BsiSI	CCGG	3 cut(s)	178, 579, 764
BslI	CCNNNNNNNGG	4 cut(s)	178, 436, 470, 770
BsmAI	GTCTC	1 cut(s)	100
BsmI	GAATGC	1 cut(s)	750
BsnI	GGCC	4 cut(s)	213, 263, 303, 763
Bso31I	GGTCTC	1 cut(s)	100
Bsp1286I	GDGCHC	1 cut(s)	287
Bsp1407I	TGTACA	1 cut(s)	342
Bsp143I	GATC	1 cut(s)	802
Bsp1720I	GCTNAGC	1 cut(s)	749
BspACI	CCGC	7 cut(s)	87, 172, 175, 220, 324, 551, 566
BspANI	GGCC	4 cut(s)	213, 263, 303, 763
BspCNI	CTCAG	1 cut(s)	741
BspDI	ATCGAT	1 cut(s)	498
BspOI	GCTAGC	1 cut(s)	86
BspTNI	GGTCTC	1 cut(s)	100
BsrDI	GCAATG	1 cut(s)	79
BsrFI	RCCGGY	1 cut(s)	578
BsrGI	TGTACA	1 cut(s)	342
BssAI	RCCGGY	1 cut(s)	578
BssECI	CCNNGG	4 cut(s)	90, 178, 208, 538
BssMI	GATC	1 cut(s)	802
BssNI	GRCGYC	1 cut(s)	51
Bst4CI	ACNGT	2 cut(s)	196, 479
Bst6I	CTCTTC	4 cut(s)	105, 122, 579, 734
BstACI	GRCGYC	1 cut(s)	51
BstAUI	TGTACA	1 cut(s)	342
BstC8I	GCNNGC	1 cut(s)	84
BstDEI	CTNAG	2 cut(s)	154, 749
BstDSI	CCRYGG	1 cut(s)	90
BstENI	CCTNNNNNAGG	1 cut(s)	434
BstF5I	GGATG	1 cut(s)	293
BstKTI	GATC	1 cut(s)	805
BstMAI	GTCTC	1 cut(s)	100
BstMBI	GATC	1 cut(s)	802
BstMWI	GCNNNNNNNGC	5 cut(s)	180, 183, 219, 260, 534
BstSCI	CCNGG	1 cut(s)	177
BstV1I	GCAGC	1 cut(s)	195
BstV2I	GAAGAC	2 cut(s)	684, 690
Bsu15I	ATCGAT	1 cut(s)	498
BsuRI	GGCC	4 cut(s)	213, 263, 303, 763
BsuTUI	ATCGAT	1 cut(s)	498
BtgI	CCRYGG	1 cut(s)	90
BtgZI	GCGATG	2 cut(s)	671, 696
BtsCI	GGATG	1 cut(s)	293
BtsIMutI	CAGTG	1 cut(s)	475
Cac8I	GCNNGC	1 cut(s)	84
Cfr10I	RCCGGY	1 cut(s)	578
Cfr13I	GGNCC	2 cut(s)	212, 542
ClaI	ATCGAT	1 cut(s)	498
CseI	GACGC	2 cut(s)	59, 260
Csp6I	GTAC	2 cut(s)	343, 663
CviAII	CATG	5 cut(s)	79, 203, 448, 485, 647
CviQI	GTAC	2 cut(s)	343, 663
DdeI	CTNAG	2 cut(s)	154, 749
DpnI	GATC	1 cut(s)	804
DpnII	GATC	1 cut(s)	802
EaeI	YGGCCR	1 cut(s)	261
Eam1104I	CTCTTC	4 cut(s)	105, 122, 579, 734
EarI	CTCTTC	4 cut(s)	105, 122, 579, 734
EciI	GGCGGA	2 cut(s)	313, 581
Ecl136II	GAGCTC	1 cut(s)	285
Eco147I	AGGCCT	1 cut(s)	303
Eco24I	GRGCYC	1 cut(s)	287
Eco31I	GGTCTC	1 cut(s)	100
Eco47I	GGWCC	1 cut(s)	542
Eco53kI	GAGCTC	1 cut(s)	285
EcoICRI	GAGCTC	1 cut(s)	285
EcoNI	CCTNNNNNAGG	1 cut(s)	434
EcoT38I	GRGCYC	1 cut(s)	287
FaeI	CATG	5 cut(s)	82, 206, 451, 488, 650
FaiI	YATR	8 cut(s)	64, 80, 204, 449, 486, 503, 624, 648
FalI	AAGNNNNNCTT	2 cut(s)	137, 169
FatI	CATG	5 cut(s)	78, 202, 447, 484, 646
FbaI	TGATCA	1 cut(s)	802
FblI	GTMKAC	1 cut(s)	48
Fnu4HI	GCNGC	3 cut(s)	87, 175, 184
FokI	GGATG	1 cut(s)	280
FriOI	GRGCYC	1 cut(s)	287
Fsp4HI	GCNGC	3 cut(s)	87, 175, 184
FspBI	CTAG	2 cut(s)	83, 305
GluI	GCNGC	3 cut(s)	87, 175, 184
GsaI	CCCAGC	1 cut(s)	512
GsuI	CTGGAG	1 cut(s)	380
HaeIII	GGCC	4 cut(s)	213, 263, 303, 763
HapII	CCGG	3 cut(s)	178, 579, 764
HgaI	GACGC	2 cut(s)	59, 260
Hin1I	GRCGYC	1 cut(s)	51
Hin1II	CATG	5 cut(s)	82, 206, 451, 488, 650
HincII	GTYRAC	1 cut(s)	49
HindII	GTYRAC	1 cut(s)	49
HinfI	GANTC	4 cut(s)	308, 590, 614, 674
HpaII	CCGG	3 cut(s)	178, 579, 764
HphI	GGTGA	2 cut(s)	184, 593
Hpy166II	GTNNAC	2 cut(s)	49, 343
Hpy188I	TCNGA	1 cut(s)	546
Hpy188III	TCNNGA	1 cut(s)	226
Hpy8I	GTNNAC	2 cut(s)	49, 343
Hpy99I	CGWCG	3 cut(s)	50, 53, 565
HpyAV	CCTTC	3 cut(s)	250, 447, 693
HpyCH4III	ACNGT	2 cut(s)	196, 479
HpyF10VI	GCNNNNNNNGC	5 cut(s)	180, 183, 219, 260, 534
HpyF3I	CTNAG	2 cut(s)	154, 749
Hsp92I	GRCGYC	1 cut(s)	51
Hsp92II	CATG	5 cut(s)	82, 206, 451, 488, 650
Ksp22I	TGATCA	1 cut(s)	802
Kzo9I	GATC	1 cut(s)	802
LmnI	GCTCC	3 cut(s)	191, 355, 653
LpnPI	CCDG	8 cut(s)	191, 239, 344, 443, 494, 592, 603, 777
Lsp1109I	GCAGC	1 cut(s)	195
LweI	GCATC	1 cut(s)	802
MaeI	CTAG	2 cut(s)	83, 305
MaeIII	GTNAC	2 cut(s)	190, 473
MalI	GATC	1 cut(s)	804
MboI	GATC	1 cut(s)	802
MboII	GAAGA	7 cut(s)	92, 95, 109, 596, 684, 690, 751
MhlI	GDGCHC	1 cut(s)	287
MluCI	AATT	4 cut(s)	382, 417, 531, 631
MlyI	GAGTC	1 cut(s)	302
MmeI	TCCRAC	1 cut(s)	631
MroXI	GAANNNNTTC	1 cut(s)	221
MseI	TTAA	1 cut(s)	719
MspI	CCGG	3 cut(s)	178, 579, 764
MspR9I	CCNGG	1 cut(s)	179
Mva1269I	GAATGC	1 cut(s)	750
MwoI	GCNNNNNNNGC	5 cut(s)	180, 183, 219, 260, 534
NciI	CCSGG	1 cut(s)	179
NdeII	GATC	1 cut(s)	802
NheI	GCTAGC	1 cut(s)	82
NlaIII	CATG	5 cut(s)	82, 206, 451, 488, 650
NmeAIII	GCCGAG	3 cut(s)	189, 239, 563
NmuCI	GTSAC	2 cut(s)	190, 473
PceI	AGGCCT	1 cut(s)	303
PcsI	WCGNNNNNNNCGW	3 cut(s)	441, 495, 701
PctI	GAATGC	1 cut(s)	750
PdmI	GAANNNNTTC	1 cut(s)	221
PfeI	GAWTC	3 cut(s)	590, 614, 674
PkrI	GCNGC	3 cut(s)	88, 176, 185
PleI	GAGTC	1 cut(s)	302
PpsI	GAGTC	1 cut(s)	302
PshBI	ATTAAT	1 cut(s)	719
Psp124BI	GAGCTC	1 cut(s)	287
PspFI	CCCAGC	1 cut(s)	508
PspPI	GGNCC	2 cut(s)	212, 542
RsaI	GTAC	2 cut(s)	344, 664
RsaNI	GTAC	2 cut(s)	343, 663
SacI	GAGCTC	1 cut(s)	287
SalI	GTCGAC	1 cut(s)	47
SaqAI	TTAA	1 cut(s)	719
SatI	GCNGC	3 cut(s)	87, 175, 184
Sau3AI	GATC	1 cut(s)	802
Sau96I	GGNCC	2 cut(s)	212, 542
SchI	GAGTC	1 cut(s)	302
ScrFI	CCNGG	1 cut(s)	179
SduI	GDGCHC	1 cut(s)	287
SetI	ASST	8 cut(s)	160, 188, 242, 287, 360, 544, 685, 780
SfaNI	GCATC	1 cut(s)	802
SgrDI	CGTCGACG	1 cut(s)	47
SinI	GGWCC	1 cut(s)	542
SmlI	CTYRAG	1 cut(s)	159
SmoI	CTYRAG	1 cut(s)	159
Sse9I	AATT	4 cut(s)	382, 417, 531, 631
SseBI	AGGCCT	1 cut(s)	303
SsiI	CCGC	7 cut(s)	87, 172, 175, 220, 324, 551, 566
SspMI	CTAG	2 cut(s)	83, 305
SstI	GAGCTC	1 cut(s)	287
StuI	AGGCCT	1 cut(s)	303
StyD4I	CCNGG	1 cut(s)	177
TaaI	ACNGT	2 cut(s)	196, 479
TaqI	TCGA	7 cut(s)	48, 281, 311, 435, 489, 498, 677
TasI	AATT	4 cut(s)	382, 417, 531, 631
TatI	WGTACW	1 cut(s)	342
TauI	GCSGC	2 cut(s)	89, 177
TfiI	GAWTC	3 cut(s)	590, 614, 674
Tru1I	TTAA	1 cut(s)	719
Tru9I	TTAA	1 cut(s)	719
TscAI	CASTG	1 cut(s)	482
TseFI	GTSAC	2 cut(s)	190, 473
TseI	GCWGC	1 cut(s)	183
Tsp45I	GTSAC	2 cut(s)	190, 473
TspDTI	ATGAA	5 cut(s)	51, 282, 635, 675, 723
TspGWI	ACGGA	1 cut(s)	178
TspRI	CASTG	1 cut(s)	482
VpaK11BI	GGWCC	1 cut(s)	542
VspI	ATTAAT	1 cut(s)	719
XagI	CCTNNNNNAGG	1 cut(s)	434
XmiI	GTMKAC	1 cut(s)	48
XmnI	GAANNNNTTC	1 cut(s)	221
XspI	CTAG	2 cut(s)	83, 305

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.