MD03G1070300.v1.1

Protein of unknown function (DUF679)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0013979
Physical Location & Seq
Forward (+)
5736090 .. 5737828
1739 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1070300.v1.1.491

Sequence Viewer

Length: 648 bp
ATGGCAGCAGTCGAGGAAGCACAGCAACCACTTTTGGATAATAATGCAGGAGGAGGAGGAGAAGGATCAAGTTCGACTGCCCCAAAGCCAAAACAACCAAAAACCCCGGCACAAAAGGCCATTAGAAAGACCTTCAAAGCCACGGCCCATTTGTCTAATCTTCTCCCCACGGGGACCGTCCTCGTGTTCCAAATGCTCTCCCCCGCTTTCACATGCCAAGGGAAGTGCCCGGCCATCCTCAACCAAACCGTGACTCTGCTCCTCCTAACGTTCTGCAGCCTTACCTGCTTCCTTTTGTGCTTGACCGATAGCTTGAGGGACGAACGAGGCAAGGTGCGATATGGGTTGGCAACATTTAAAGGGTTGTGGATCCTCGACGGGTCCAAAATCACACTTTCGCCGGAGGAGGCAGCTAAATATAGGCTAAAGTTCATAGATTTTTTCCATGCCTTCATGTCCATACTTGTATTTGCTGCGGTGGCAATGTTGGATAAAAATGTCGTCAATTGCTTCTACCCCAAGCTATCGGAGGAGGCCAGGCAGCTTTTGGTGGCGTTCCATGTCGGAACTGGTATCGTATGCAGCTTGCTGTTTGTTTTATTCCCCTCCAGACGCCATGGAATTGGCTTCCCTCTCTCTCGTAGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF679

Protein Analysis

216

Amino Acids

23.44

Weight (kDa)

9.35

Isoelectric Point (pI)

44.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF679 PF05078 47 - 210 1.2e-65 Protein of unknown function (DUF679)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013979)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G28485 AT4G28485
fragaria_vesca FvH4_6g26701
malus_domestica MD03G1070300.v1.1 MD11G1074100.v1.1
prunus_persica Prupe.4G053000_v2.0.a1
pyrus_communis pycom11g06150
rosa_chinensis RchiOBHm_Chr0c19g0500031
rosa_laevigata RLG00000018985
rosa_multiflora Rmu_sc0008568.1_g000003
rosa_roxburghii Rroxscaffold_2G00115750
rosa_rugosa Rorug02G0277600
rosa_samantha Rh2AG328200 Rh2BG338300 Rh2CG315300 Rh2DG355200
rosa_wichuraiana Rw2G026620

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 293
AciI CCGC 2 cut(s) 204, 476
AclI AACGTT 1 cut(s) 269
AclWI GGATC 3 cut(s) 73, 364, 377
AcoI YGGCCR 1 cut(s) 231
AcyI GRCGYC 1 cut(s) 613
AgsI TTSAA 1 cut(s) 136
AjnI CCWGG 1 cut(s) 536
AluBI AGCT 6 cut(s) 312, 413, 523, 544, 585, 645
AluI AGCT 6 cut(s) 312, 413, 523, 544, 585, 645
AlwI GGATC 3 cut(s) 73, 364, 377
AoxI GGCC 4 cut(s) 117, 144, 231, 534
ApeKI GCWGC 6 cut(s) 5, 276, 410, 473, 541, 582
AspS9I GGNCC 3 cut(s) 145, 174, 381
AsuC2I CCSGG 2 cut(s) 107, 230
AvaII GGWCC 2 cut(s) 174, 381
BaeGI GKGCMC 1 cut(s) 230
BamHI GGATCC 1 cut(s) 369
BauI CACGAG 1 cut(s) 182
BbvI GCAGC 6 cut(s) 17, 288, 422, 460, 553, 594
BccI CCATC 1 cut(s) 242
BceAI ACGGC 1 cut(s) 159
BciT130I CCWGG 1 cut(s) 538
BcnI CCSGG 2 cut(s) 107, 230
BfaI CTAG 1 cut(s) 646
BfmI CTRYAG 1 cut(s) 274
BfuAI ACCTGC 1 cut(s) 293
BisI GCNGC 6 cut(s) 6, 277, 411, 474, 542, 583
BlsI GCNGC 6 cut(s) 7, 278, 412, 475, 543, 584
Bme1390I CCNGG 3 cut(s) 107, 230, 538
Bme18I GGWCC 2 cut(s) 174, 381
BmgT120I GGNCC 3 cut(s) 145, 174, 381
BmiI GGNNCC 3 cut(s) 175, 371, 382
BmrFI CCNGG 3 cut(s) 107, 230, 538
BpmI CTGGAG 1 cut(s) 592
BpuEI CTTGAG 1 cut(s) 334
BpuMI CCSGG 2 cut(s) 107, 230
BsaHI GRCGYC 1 cut(s) 613
BsaJI CCNNGG 5 cut(s) 105, 141, 168, 217, 616
Bse1I ACTGG 1 cut(s) 574
Bse3DI GCAATG 1 cut(s) 489
BseBI CCWGG 1 cut(s) 538
BseDI CCNNGG 5 cut(s) 105, 141, 168, 217, 616
BseGI GGATG 1 cut(s) 234
BseMI GCAATG 1 cut(s) 489
BseNI ACTGG 1 cut(s) 574
BseRI GAGGAG 6 cut(s) 66, 69, 72, 251, 419, 545
BseSI GKGCMC 1 cut(s) 230
BseXI GCAGC 6 cut(s) 17, 288, 422, 460, 553, 594
BshFI GGCC 4 cut(s) 119, 146, 233, 536
BsiSI CCGG 3 cut(s) 107, 230, 401
BslFI GGGAC 2 cut(s) 187, 332
BsmFI GGGAC 2 cut(s) 187, 332
BsnI GGCC 4 cut(s) 119, 146, 233, 536
Bsp1286I GDGCHC 1 cut(s) 230
Bsp143I GATC 2 cut(s) 65, 369
Bsp19I CCATGG 1 cut(s) 616
BspACI CCGC 2 cut(s) 204, 476
BspANI GGCC 4 cut(s) 119, 146, 233, 536
BspLI GGNNCC 3 cut(s) 175, 371, 382
BspMAI CTGCAG 1 cut(s) 278
BspMI ACCTGC 1 cut(s) 293
BspPI GGATC 3 cut(s) 73, 364, 377
BsrDI GCAATG 1 cut(s) 489
BsrI ACTGG 1 cut(s) 574
BssECI CCNNGG 5 cut(s) 105, 141, 168, 217, 616
BssMI GATC 2 cut(s) 65, 369
BssNI GRCGYC 1 cut(s) 613
BssSI CACGAG 1 cut(s) 182
BssT1I CCWWGG 2 cut(s) 217, 616
Bst2BI CACGAG 1 cut(s) 182
Bst2UI CCWGG 1 cut(s) 538
Bst4CI ACNGT 2 cut(s) 178, 250
BstACI GRCGYC 1 cut(s) 613
BstC8I GCNNGC 1 cut(s) 587
BstDSI CCRYGG 3 cut(s) 141, 168, 616
BstF5I GGATG 1 cut(s) 234
BstKTI GATC 2 cut(s) 68, 372
BstMBI GATC 2 cut(s) 65, 369
BstMWI GCNNNNNNNGC 3 cut(s) 116, 285, 479
BstNI CCWGG 1 cut(s) 538
BstNSI RCATGY 1 cut(s) 216
BstSCI CCNGG 3 cut(s) 105, 228, 536
BstSFI CTRYAG 1 cut(s) 274
BstSLI GKGCMC 1 cut(s) 230
BstV1I GCAGC 6 cut(s) 17, 288, 422, 460, 553, 594
BstX2I RGATCY 1 cut(s) 369
BstXI CCANNNNNNTGG 1 cut(s) 623
BstYI RGATCY 1 cut(s) 369
BsuRI GGCC 4 cut(s) 119, 146, 233, 536
BtgI CCRYGG 3 cut(s) 141, 168, 616
BtsCI GGATG 1 cut(s) 234
BveI ACCTGC 1 cut(s) 293
Cac8I GCNNGC 1 cut(s) 587
Cfr13I GGNCC 3 cut(s) 145, 174, 381
CseI GACGC 1 cut(s) 621
CviAII CATG 5 cut(s) 213, 446, 454, 560, 617
DpnI GATC 2 cut(s) 67, 371
DpnII GATC 2 cut(s) 65, 369
DraI TTTAAA 1 cut(s) 358
EaeI YGGCCR 1 cut(s) 231
Eco130I CCWWGG 2 cut(s) 217, 616
Eco47I GGWCC 2 cut(s) 174, 381
EcoRII CCWGG 1 cut(s) 536
EcoT14I CCWWGG 2 cut(s) 217, 616
ErhI CCWWGG 2 cut(s) 217, 616
FaeI CATG 5 cut(s) 216, 449, 457, 563, 620
FaqI GGGAC 2 cut(s) 187, 332
FatI CATG 5 cut(s) 212, 445, 453, 559, 616
FauI CCCGC 1 cut(s) 211
Fnu4HI GCNGC 6 cut(s) 6, 277, 411, 474, 542, 583
FokI GGATG 1 cut(s) 221
Fsp4HI GCNGC 6 cut(s) 6, 277, 411, 474, 542, 583
FspBI CTAG 1 cut(s) 646
GluI GCNGC 6 cut(s) 6, 277, 411, 474, 542, 583
GsuI CTGGAG 1 cut(s) 592
HaeIII GGCC 4 cut(s) 119, 146, 233, 536
HapII CCGG 3 cut(s) 107, 230, 401
HgaI GACGC 1 cut(s) 621
Hin1I GRCGYC 1 cut(s) 613
Hin1II CATG 5 cut(s) 216, 449, 457, 563, 620
HinfI GANTC 1 cut(s) 253
HpaII CCGG 3 cut(s) 107, 230, 401
Hpy188I TCNGA 2 cut(s) 529, 566
Hpy188III TCNNGA 1 cut(s) 609
Hpy99I CGWCG 1 cut(s) 380
HpyAV CCTTC 3 cut(s) 56, 142, 460
HpyCH4III ACNGT 2 cut(s) 178, 250
HpyCH4IV ACGT 1 cut(s) 269
HpyCH4V TGCA 3 cut(s) 47, 276, 582
HpyF10VI GCNNNNNNNGC 3 cut(s) 116, 285, 479
HpySE526I ACGT 1 cut(s) 269
Hsp92I GRCGYC 1 cut(s) 613
Hsp92II CATG 5 cut(s) 216, 449, 457, 563, 620
Kzo9I GATC 2 cut(s) 65, 369
LmnI GCTCC 1 cut(s) 264
LpnPI CCDG 9 cut(s) 33, 120, 243, 298, 414, 523, 550, 555, 622
Lsp1109I GCAGC 6 cut(s) 17, 288, 422, 460, 553, 594
MaeI CTAG 1 cut(s) 646
MaeII ACGT 1 cut(s) 269
MaeIII GTNAC 1 cut(s) 250
MalI GATC 2 cut(s) 67, 371
MboI GATC 2 cut(s) 65, 369
MboII GAAGA 1 cut(s) 152
MfeI CAATTG 1 cut(s) 505
MflI RGATCY 1 cut(s) 369
MhlI GDGCHC 1 cut(s) 230
MluCI AATT 2 cut(s) 505, 621
MlyI GAGTC 1 cut(s) 247
MmeI TCCRAC 2 cut(s) 468, 544
MseI TTAA 1 cut(s) 357
MspI CCGG 3 cut(s) 107, 230, 401
MspR9I CCNGG 3 cut(s) 107, 230, 538
MunI CAATTG 1 cut(s) 505
MvaI CCWGG 1 cut(s) 538
MwoI GCNNNNNNNGC 3 cut(s) 116, 285, 479
NciI CCSGG 2 cut(s) 107, 230
NcoI CCATGG 1 cut(s) 616
NdeII GATC 2 cut(s) 65, 369
NlaIII CATG 5 cut(s) 216, 449, 457, 563, 620
NlaIV GGNNCC 3 cut(s) 175, 371, 382
NmuCI GTSAC 1 cut(s) 250
NspI RCATGY 1 cut(s) 216
PkrI GCNGC 6 cut(s) 7, 278, 412, 475, 543, 584
PleI GAGTC 1 cut(s) 247
PpsI GAGTC 1 cut(s) 247
Psp1406I AACGTT 1 cut(s) 269
Psp6I CCWGG 1 cut(s) 536
PspGI CCWGG 1 cut(s) 536
PspN4I GGNNCC 3 cut(s) 175, 371, 382
PspPI GGNCC 3 cut(s) 145, 174, 381
PstI CTGCAG 1 cut(s) 278
PsuI RGATCY 1 cut(s) 369
SaqAI TTAA 1 cut(s) 357
SatI GCNGC 6 cut(s) 6, 277, 411, 474, 542, 583
Sau3AI GATC 2 cut(s) 65, 369
Sau96I GGNCC 3 cut(s) 145, 174, 381
SchI GAGTC 1 cut(s) 247
ScrFI CCNGG 3 cut(s) 107, 230, 538
SduI GDGCHC 1 cut(s) 230
SfcI CTRYAG 1 cut(s) 274
SinI GGWCC 2 cut(s) 174, 381
SmlI CTYRAG 1 cut(s) 313
SmoI CTYRAG 1 cut(s) 313
Sse9I AATT 2 cut(s) 505, 621
SsiI CCGC 2 cut(s) 204, 476
SspMI CTAG 1 cut(s) 646
StyD4I CCNGG 3 cut(s) 105, 228, 536
StyI CCWWGG 2 cut(s) 217, 616
TaaI ACNGT 2 cut(s) 178, 250
TaiI ACGT 1 cut(s) 272
TaqI TCGA 3 cut(s) 12, 74, 375
TaqII GACCGA 1 cut(s) 320
TasI AATT 2 cut(s) 505, 621
Tru1I TTAA 1 cut(s) 357
Tru9I TTAA 1 cut(s) 357
TseFI GTSAC 1 cut(s) 250
TseI GCWGC 6 cut(s) 5, 276, 410, 473, 541, 582
Tsp45I GTSAC 1 cut(s) 250
TspDTI ATGAA 2 cut(s) 421, 442
VpaK11BI GGWCC 2 cut(s) 174, 381
XceI RCATGY 1 cut(s) 216
XcmI CCANNNNNNNNNTGG 2 cut(s) 544, 566
XspI CTAG 1 cut(s) 646
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.