Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr03

Orthogroup ID

OG0002488

Physical Location & Seq

Genomic Coordinates Reverse (-)

6005939 .. 6009489

Gene Structure 3551 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD03G1074000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2100 bp

ATGCTACAACGTAATCCTAGGCAGGTGCTCAGATTGTCTTTGAAATTTTCCGTAGTGAGAGAACAAGATAAGACTGTGAGAAGCCTCCTTCTTCTACCAGCCCCAGGTTCCAGGTTCCAGGAAATTGATATGGCATCGGTGCATAGTGCCCTGACACCGGTTGTGTGCAAGAATGGCAGTCATTCTTCTGCAGCAAAGTTCCCGACGACTACCTTCTTGCCCGGGTTTGATGCAGTTGGGCGTCTTTCAAGTCCGTTTAAGAAGGAAGTGTGCCTTAGTTCCCCGAGCTCAGGTGCTAAAGCTACACTAACCTTTGATCCCCCAGCAACCAATTCTGAGAAACCCAAACTACCAAGGCATACAGTTGATCCCGCTTCTCCAGATTTTCTGCCTCTTCCATCTTTCGAACAATGTTTTCCCAAGAGCACAAAAGAACACATGGAAGTTGTTCATGAAGAAACTGGTCATGTGCTAAAAGTTCCCTTTCGACGAGTCCACCTGGCTGGGGATGAACCTCCATTTGACAACTATGATACCAGTGGTCCACAAAACATTAGCCCGCGTGTTGGACTCCCTGAACTGCGGAAAGATTGGATTGAGAGGCGAGACAAATTAGGTGCACCAAGATACACTCAGATGTACTATGCTAAGCAGGGAATTATAACTGAGGAGATGTTGTACTGCGCCACTCGTGAGAAGCTTGATCCAGAGTTTGTGAGATCAGAAGTAGCTCGTGGGCGGGCAATTATCCCTTCCAATAAGAAACACTTGGAGCTGGAGCCAATGATTGTCGGAAGAAAATTTTTGGTCAAAGTTAATGCAAACATTGGAAATTCTGCTGTTGCCAGCTCTATCGAAGAGGAAGTTTACAAGGTCCAATGGGCAACTATGTGGGGTGCTGACACTGTTATGGACCTCTCTACAGGACGCCACATCCATGAGACCCGTGAATGGATCCTGCGTAACTCTCCTGTGCCAGTAGGGACCGTACCCATTTATCAAGCGCTTGAAAAAGTGGACGGAATTGCAGAAAACCTTAACTGGGAAGTGTTCAGAGAAACTCTTATTGAACAAGCCGAGCAGGGTGTAGATTACTTCACCATCCATGCTGGGGTTCTACTACGGTACATCCCACTAACAGCAAAGCGTATGACTGGTATTGTTTCACGAGGAGGATCCATTCATGCAAAGTGGTGCTTAGCTTATCACAAAGAGAATTTCGCTTACGAGCACTGGGATGACATACTTGACATCTGCAATCAATATGATGTGGCCCTGTCAATTGGTGACGGGCTGAGACCTGGTTCCATTTATGATGCCAACGACACTGCACAGTTCGCAGAACTCTTAACTCAGGGCGAACTGACCCGTAGGGCATGGGAAAAGGATGTTCAGGTCATGAATGAAGGACCTGGACATGTTCCAATGCATAAAATTCCTGAAAACATGAAAAAACAGCTTGAATGGTGTAACGAAGCGCCTTTCTACACTCTTGGTCCTCTAACAACTGATATTGCTCCAGGATATGATCACATCACCTCTGCCATTGGTGCTGCCAACATTGGGGCTCTAGGCACCGCCCTTCTCTGTTATGTAACCCCAAAAGAGCACCTTGGGTTGCCAAACCGTGATGATGTGAAGGCTGGAGTTATAGCATATAAGATAGCTGCTCATGCCGCTGATCTAGCCAAAGGTCACCCATATGCTCAAGCCTGGGATGATGCACTGAGCAAGGCAAGATTTGAGTTCCGGTGGATGGACCAGTTCGCCTTGTCACTAGACCCTATGACTGCCATGTCATTCCATGATGAAACCCTGCCAGCTGATGGTGCCAAGGTGGCCCATTTTTGCTCAATGTGCGGTCCAAAGTTCTGTTCTATGAAGATAACGGAGGACGTGAGGAAGTACGCTGAAGAGCATGGTTACGGGAGTGCTGAGGAAGCTGTGATTCGTGGAATGGATGCTATGAGTGCTGAGTTTCTTGCTGCCAAGAAAACTGTCAGTGGAGAACAACATGGTGAAGTTGGGGGGGAAATTTACTTGCCAGAAAGTTATGTGAAAGCCGCAGAGAGGTCCACAGAGAGTTGCAGGTTTGCGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006725 GO:0006766 GO:0006767 GO:0006772 GO:0006790 GO:0006807 GO:0007584 GO:0008150 GO:0008152 GO:0009058 GO:0009110 GO:0009228 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009605 GO:0009987 GO:0009991 GO:0010033 GO:0010243 GO:0010266 GO:0014070 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0017144 GO:0018130 GO:0019144 GO:0019438 GO:0019904 GO:0031667 GO:0033273 GO:0034641 GO:0042221 GO:0042364 GO:0042493 GO:0042723 GO:0042724 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044249 GO:0044271 GO:0044272 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0046483 GO:0048037 GO:0050896 GO:0051536 GO:0051540 GO:0071704 GO:0072527 GO:0072528 GO:0080041 GO:1901360 GO:1901362 GO:1901564 GO:1901566 GO:1901576 GO:1901698

KEGG Pathways

Metabolic & Signaling

ko00730 ko01100 map00730 map01100

Pfam Domains

Protein Families

ThiC-associated ThiC_Rad_SAM

Protein Analysis

700

Amino Acids

77.82

Weight (kDa)

6.03

Isoelectric Point (pI)

45.35

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
ThiC_Rad_SAM	PF01964	211 - 631	5.7e-184	Radical SAM ThiC family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0002488)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G29630 AT2G29630 AT2G29630 AT2G29630
fragaria_vesca	FvH4_3g38200 FvH4_3g38200 FvH4_3g38200 FvH4_3g38200
malus_domestica	MD03G1074000.v1.1 MD11G1080300.v1.1
prunus_persica	Prupe.6G060700_v2.0.a1 Prupe.6G060700_v2.0.a1 Prupe.6G060700_v2.0.a1 Prupe.6G060700_v2.0.a1 Prupe.6G060700_v2.0.a1 Prupe.6G060700_v2.0.a1 Prupe.6G060700_v2.0.a1 Prupe.6G060700_v2.0.a1 Prupe.6G060700_v2.0.a1 Prupe.6G060700_v2.0.a1 Prupe.6G060700_v2.0.a1
pyrus_communis	pycom03g05980 pycom11g06760
rosa_chinensis	RchiOBHm_Chr5g0068641 RchiOBHm_Chr5g0068681
rosa_laevigata	RLG00000035986 RLG00000035996
rosa_multiflora	Rmu_sc0000376.1_g000006
rosa_roxburghii	Rroxscaffold_1G00012160 Rroxscaffold_1G00012240
rosa_rugosa	Rorug05G0392100 Rorug05G0392200 Rorug05G0392200 Rorug05G0392200 Rorug05G0392200 Rorug05G0392200
rosa_samantha	Rh5AG449800 Rh5BG468000 Rh5CG489900 Rh5DG481300
rosa_wichuraiana	Rw5G041990

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	662
AarI	CACCTGC	1 cut(s)	13
AasI	GACNNNNNNGTC	1 cut(s)	1795
Acc36I	ACCTGC	2 cut(s)	13, 2079
AccB1I	GGYRCC	2 cut(s)	1574, 1829
AccB7I	CCANNNNNTGG	1 cut(s)	1826
AccII	CGCG	1 cut(s)	562
AciI	CCGC	8 cut(s)	372, 560, 583, 739, 1578, 1677, 1860, 2064
AclWI	GGATC	7 cut(s)	311, 362, 698, 949, 962, 1170, 1183
AcsI	RAATTY	6 cut(s)	44, 800, 832, 1216, 1434, 2034
AcuI	CTGAAG	1 cut(s)	1932
AcyI	GRCGYC	2 cut(s)	241, 928
AfaI	GTAC	5 cut(s)	641, 680, 990, 1127, 1907
AfeI	AGCGCT	1 cut(s)	1005
AflIII	ACRYGT	1 cut(s)	1417
AgeI	ACCGGT	1 cut(s)	157
AgsI	TTSAA	5 cut(s)	43, 249, 1010, 1070, 1463
AjiI	CACGTC	1 cut(s)	1897
AjnI	CCWGG	8 cut(s)	103, 110, 117, 498, 1300, 1411, 1519, 1712
AjuI	GAANNNNNNNTTGG	2 cut(s)	1858, 1890
Alw21I	GWGCWC	6 cut(s)	30, 290, 428, 622, 1233, 1611
Alw26I	GTCTC	3 cut(s)	600, 935, 1291
Alw44I	GTGCAC	1 cut(s)	618
AlwI	GGATC	7 cut(s)	311, 362, 698, 949, 962, 1170, 1183
Ama87I	CYCGRG	2 cut(s)	221, 283
Aor51HI	AGCGCT	1 cut(s)	1005
AoxI	GGCC	2 cut(s)	1272, 1839
ApaLI	GTGCAC	1 cut(s)	618
ApeKI	GCWGC	4 cut(s)	191, 1553, 1667, 1985
ApoI	RAATTY	6 cut(s)	44, 800, 832, 1216, 1434, 2034
AsiGI	ACCGGT	1 cut(s)	157
Asp700I	GAANNNNTTC	1 cut(s)	447
AspA2I	CCTAGG	1 cut(s)	17
AspLEI	GCGC	3 cut(s)	686, 1006, 1480
AsuC2I	CCSGG	2 cut(s)	222, 223
AsuHPI	GGTGA	5 cut(s)	1090, 1298, 1528, 1688, 2030
AsuII	TTCGAA	1 cut(s)	405
AvaI	CYCGRG	2 cut(s)	221, 283
AvaII	GGWCC	9 cut(s)	542, 874, 913, 984, 1409, 1496, 1759, 1862, 2073
AvrII	CCTAGG	1 cut(s)	17
BaeGI	GKGCMC	2 cut(s)	151, 622
BamHI	GGATCC	2 cut(s)	954, 1175
BanI	GGYRCC	2 cut(s)	1574, 1829
BanII	GRGCYC	2 cut(s)	290, 1570
BauI	CACGAG	3 cut(s)	690, 732, 1167
Bbv12I	GWGCWC	6 cut(s)	30, 290, 428, 622, 1233, 1611
BbvCI	CCTCAGC	1 cut(s)	1935
BbvI	GCAGC	4 cut(s)	203, 1540, 1654, 1972
BccI	CCATC	4 cut(s)	406, 1109, 1750, 1820
BciT130I	CCWGG	8 cut(s)	105, 112, 119, 500, 1302, 1413, 1521, 1714
BclI	TGATCA	1 cut(s)	1528
BcnI	CCSGG	2 cut(s)	222, 223
BcoDI	GTCTC	3 cut(s)	600, 935, 1291
BfaI	CTAG	4 cut(s)	18, 1571, 1685, 1778
BfmI	CTRYAG	2 cut(s)	189, 921
BfoI	RGCGCY	2 cut(s)	1007, 1481
BfuAI	ACCTGC	2 cut(s)	13, 2079
BglI	GCCNNNNNGGC	1 cut(s)	1838
BisI	GCNGC	6 cut(s)	192, 1554, 1668, 1677, 1986, 2064
BlnI	CCTAGG	1 cut(s)	17
BlpI	GCTNAGC	2 cut(s)	648, 1198
BlsI	GCNGC	6 cut(s)	193, 1555, 1669, 1678, 1987, 2065
Bme18I	GGWCC	9 cut(s)	542, 874, 913, 984, 1409, 1496, 1759, 1862, 2073
BmeT110I	CYCGRG	2 cut(s)	221, 283
BmgBI	CACGTC	1 cut(s)	1897
BmiI	GGNNCC	9 cut(s)	109, 116, 780, 956, 985, 1177, 1306, 1576, 1831
BmrI	ACTGGG	2 cut(s)	1051, 1243
BmsI	GCATC	5 cut(s)	143, 220, 1306, 1711, 1951
BmuI	ACTGGG	2 cut(s)	1051, 1243
BpmI	CTGGAG	4 cut(s)	363, 797, 1503, 1665
Bpu10I	CCTNAGC	2 cut(s)	289, 1935
Bpu1102I	GCTNAGC	2 cut(s)	648, 1198
Bpu14I	TTCGAA	1 cut(s)	405
BpuEI	CTTGAG	1 cut(s)	1692
BpuMI	CCSGG	2 cut(s)	222, 223
BsaHI	GRCGYC	2 cut(s)	241, 928
BsaI	GGTCTC	2 cut(s)	935, 1291
BsaJI	CCNNGG	7 cut(s)	17, 103, 221, 353, 1612, 1713, 1833
BsaWI	WCCGGW	2 cut(s)	157, 1749
BsaXI	ACNNNNNCTCC	2 cut(s)	662, 692
Bse118I	RCCGGY	1 cut(s)	157
Bse1I	ACTGG	7 cut(s)	466, 537, 977, 1046, 1159, 1238, 1762
BseBI	CCWGG	8 cut(s)	105, 112, 119, 500, 1302, 1413, 1521, 1714
BseDI	CCNNGG	7 cut(s)	17, 103, 221, 353, 1612, 1713, 1833
BseGI	GGATG	9 cut(s)	514, 933, 1101, 1128, 1243, 1393, 1723, 1761, 1966
BseNI	ACTGG	7 cut(s)	466, 537, 977, 1046, 1159, 1238, 1762
BseRI	GAGGAG	2 cut(s)	683, 1185
BseSI	GKGCMC	2 cut(s)	151, 622
BseXI	GCAGC	4 cut(s)	203, 1540, 1654, 1972
BseYI	CCCAGC	3 cut(s)	322, 503, 1109
BsgI	GTGCAG	1 cut(s)	1314
Bsh1236I	CGCG	1 cut(s)	562
BshFI	GGCC	2 cut(s)	1274, 1841
BshNI	GGYRCC	2 cut(s)	1574, 1829
BshTI	ACCGGT	1 cut(s)	157
BsiHKAI	GWGCWC	6 cut(s)	30, 290, 428, 622, 1233, 1611
BsiHKCI	CYCGRG	2 cut(s)	221, 283
BsiSI	CCGG	3 cut(s)	158, 222, 1750
BslFI	GGGAC	1 cut(s)	997
BsmAI	GTCTC	3 cut(s)	600, 935, 1291
BsmFI	GGGAC	1 cut(s)	997
BsnI	GGCC	2 cut(s)	1274, 1841
Bso31I	GGTCTC	2 cut(s)	935, 1291
BsoBI	CYCGRG	2 cut(s)	221, 283
Bsp119I	TTCGAA	1 cut(s)	405
Bsp1286I	GDGCHC	8 cut(s)	30, 151, 290, 428, 622, 1233, 1570, 1611
Bsp143I	GATC	8 cut(s)	316, 367, 703, 719, 954, 1175, 1528, 1681
Bsp1720I	GCTNAGC	2 cut(s)	648, 1198
BspACI	CCGC	8 cut(s)	372, 560, 583, 739, 1578, 1677, 1860, 2064
BspANI	GGCC	2 cut(s)	1274, 1841
BspFNI	CGCG	1 cut(s)	562
BspHI	TCATGA	2 cut(s)	451, 1398
BspLI	GGNNCC	9 cut(s)	109, 116, 780, 956, 985, 1177, 1306, 1576, 1831
BspMAI	CTGCAG	1 cut(s)	193
BspMI	ACCTGC	2 cut(s)	13, 2079
BspPI	GGATC	7 cut(s)	311, 362, 698, 949, 962, 1170, 1183
BspQI	GCTCTTC	1 cut(s)	1908
BspT104I	TTCGAA	1 cut(s)	405
BspT107I	GGYRCC	2 cut(s)	1574, 1829
BspTNI	GGTCTC	2 cut(s)	935, 1291
BsrFI	RCCGGY	1 cut(s)	157
BsrI	ACTGG	7 cut(s)	466, 537, 977, 1046, 1159, 1238, 1762
BssAI	RCCGGY	1 cut(s)	157
BssECI	CCNNGG	7 cut(s)	17, 103, 221, 353, 1612, 1713, 1833
BssMI	GATC	8 cut(s)	316, 367, 703, 719, 954, 1175, 1528, 1681
BssNI	GRCGYC	2 cut(s)	241, 928
BssSI	CACGAG	3 cut(s)	690, 732, 1167
BssT1I	CCWWGG	4 cut(s)	17, 353, 1612, 1833
Bst2BI	CACGAG	3 cut(s)	690, 732, 1167
Bst2UI	CCWGG	8 cut(s)	105, 112, 119, 500, 1302, 1413, 1521, 1714
Bst4CI	ACNGT	8 cut(s)	76, 364, 907, 988, 1125, 1335, 1628, 1999
Bst6I	CTCTTC	3 cut(s)	399, 852, 1908
BstACI	GRCGYC	2 cut(s)	241, 928
BstBI	TTCGAA	1 cut(s)	405
BstC8I	GCNNGC	4 cut(s)	560, 741, 847, 1821
BstEII	GGTNACC	1 cut(s)	1694
BstF5I	GGATG	9 cut(s)	514, 933, 1101, 1128, 1243, 1393, 1723, 1761, 1966
BstFNI	CGCG	1 cut(s)	562
BstH2I	RGCGCY	2 cut(s)	1007, 1481
BstHHI	GCGC	3 cut(s)	686, 1006, 1480
BstKTI	GATC	8 cut(s)	319, 370, 706, 722, 957, 1178, 1531, 1684
BstMAI	GTCTC	3 cut(s)	600, 935, 1291
BstMBI	GATC	8 cut(s)	316, 367, 703, 719, 954, 1175, 1528, 1681
BstNI	CCWGG	8 cut(s)	105, 112, 119, 500, 1302, 1413, 1521, 1714
BstNSI	RCATGY	1 cut(s)	1421
BstPI	GGTNACC	1 cut(s)	1694
BstSFI	CTRYAG	2 cut(s)	189, 921
BstSLI	GKGCMC	2 cut(s)	151, 622
BstUI	CGCG	1 cut(s)	562
BstV1I	GCAGC	4 cut(s)	203, 1540, 1654, 1972
BstX2I	RGATCY	2 cut(s)	954, 1175
BstXI	CCANNNNNNTGG	1 cut(s)	503
BstYI	RGATCY	2 cut(s)	954, 1175
BsuRI	GGCC	2 cut(s)	1274, 1841
BtrI	CACGTC	1 cut(s)	1897
BtsCI	GGATG	9 cut(s)	514, 933, 1101, 1128, 1243, 1393, 1723, 1761, 1966
BtsI	GCAGTG	1 cut(s)	1326
BtsIMutI	CAGTG	6 cut(s)	544, 903, 1231, 1326, 1724, 2008
BveI	ACCTGC	2 cut(s)	13, 2079
Cac8I	GCNNGC	4 cut(s)	560, 741, 847, 1821
CciI	TCATGA	2 cut(s)	451, 1398
CfoI	GCGC	3 cut(s)	686, 1006, 1480
Cfr10I	RCCGGY	1 cut(s)	157
Cfr9I	CCCGGG	1 cut(s)	221
CseI	GACGC	2 cut(s)	230, 936
CsiI	ACCWGGT	1 cut(s)	1300
Csp6I	GTAC	5 cut(s)	640, 679, 989, 1126, 1906
CspAI	ACCGGT	1 cut(s)	157
CviQI	GTAC	5 cut(s)	640, 679, 989, 1126, 1906
DpnI	GATC	8 cut(s)	318, 369, 705, 721, 956, 1177, 1530, 1683
DpnII	GATC	8 cut(s)	316, 367, 703, 719, 954, 1175, 1528, 1681
DrdI	GACNNNNNNGTC	1 cut(s)	1795
DseDI	GACNNNNNNGTC	1 cut(s)	1795
Eam1104I	CTCTTC	3 cut(s)	399, 852, 1908
EarI	CTCTTC	3 cut(s)	399, 852, 1908
Ecl136II	GAGCTC	1 cut(s)	288
Eco130I	CCWWGG	4 cut(s)	17, 353, 1612, 1833
Eco24I	GRGCYC	2 cut(s)	290, 1570
Eco31I	GGTCTC	2 cut(s)	935, 1291
Eco47I	GGWCC	9 cut(s)	542, 874, 913, 984, 1409, 1496, 1759, 1862, 2073
Eco47III	AGCGCT	1 cut(s)	1005
Eco53kI	GAGCTC	1 cut(s)	288
Eco57I	CTGAAG	1 cut(s)	1932
Eco88I	CYCGRG	2 cut(s)	221, 283
Eco91I	GGTNACC	1 cut(s)	1694
EcoICRI	GAGCTC	1 cut(s)	288
EcoO109I	RGGNCCY	1 cut(s)	1409
EcoO65I	GGTNACC	1 cut(s)	1694
EcoRII	CCWGG	8 cut(s)	103, 110, 117, 498, 1300, 1411, 1519, 1712
EcoT14I	CCWWGG	4 cut(s)	17, 353, 1612, 1833
EcoT22I	ATGCAT	1 cut(s)	1431
EcoT38I	GRGCYC	2 cut(s)	290, 1570
ErhI	CCWWGG	4 cut(s)	17, 353, 1612, 1833
FalI	AAGNNNNNCTT	8 cut(s)	258, 290, 752, 784, 1181, 1213, 1596, 1628
FaqI	GGGAC	1 cut(s)	997
FauI	CCCGC	3 cut(s)	379, 567, 732
FauNDI	CATATG	1 cut(s)	1702
FbaI	TGATCA	1 cut(s)	1528
Fnu4HI	GCNGC	6 cut(s)	192, 1554, 1668, 1677, 1986, 2064
FokI	GGATG	9 cut(s)	521, 920, 1088, 1115, 1250, 1400, 1730, 1768, 1973
FriOI	GRGCYC	2 cut(s)	290, 1570
Fsp4HI	GCNGC	6 cut(s)	192, 1554, 1668, 1677, 1986, 2064
FspBI	CTAG	4 cut(s)	18, 1571, 1685, 1778
GlaI	GCGC	3 cut(s)	685, 1005, 1479
GluI	GCNGC	6 cut(s)	192, 1554, 1668, 1677, 1986, 2064
GsaI	CCCAGC	3 cut(s)	326, 507, 1113
GsuI	CTGGAG	4 cut(s)	363, 797, 1503, 1665
HaeII	RGCGCY	2 cut(s)	1007, 1481
HaeIII	GGCC	2 cut(s)	1274, 1841
HapII	CCGG	3 cut(s)	158, 222, 1750
HgaI	GACGC	2 cut(s)	230, 936
HhaI	GCGC	3 cut(s)	686, 1006, 1480
Hin1I	GRCGYC	2 cut(s)	241, 928
Hin6I	GCGC	3 cut(s)	684, 1004, 1478
HinP1I	GCGC	3 cut(s)	684, 1004, 1478
HindIII	AAGCTT	1 cut(s)	698
HinfI	GANTC	3 cut(s)	492, 570, 1948
HpaII	CCGG	3 cut(s)	158, 222, 1750
HphI	GGTGA	5 cut(s)	1090, 1298, 1528, 1688, 2030
Hpy166II	GTNNAC	6 cut(s)	496, 545, 620, 868, 1018, 2076
Hpy188I	TCNGA	6 cut(s)	32, 337, 636, 724, 794, 1055
Hpy188III	TCNNGA	8 cut(s)	202, 380, 452, 692, 707, 1167, 1399, 1439
Hpy8I	GTNNAC	6 cut(s)	496, 545, 620, 868, 1018, 2076
Hpy99I	CGWCG	2 cut(s)	208, 492
HpyAV	CCTTC	7 cut(s)	98, 223, 256, 762, 1400, 1592, 1633
HpyCH4III	ACNGT	8 cut(s)	76, 364, 907, 988, 1125, 1335, 1628, 1999
HpyCH4IV	ACGT	2 cut(s)	10, 1896
HpySE526I	ACGT	2 cut(s)	10, 1896
Hsp92I	GRCGYC	2 cut(s)	241, 928
HspAI	GCGC	3 cut(s)	684, 1004, 1478
Ksp22I	TGATCA	1 cut(s)	1528
Kzo9I	GATC	8 cut(s)	316, 367, 703, 719, 954, 1175, 1528, 1681
LguI	GCTCTTC	1 cut(s)	1908
LmnI	GCTCC	3 cut(s)	772, 778, 1522
Lsp1109I	GCAGC	4 cut(s)	203, 1540, 1654, 1972
LweI	GCATC	5 cut(s)	143, 220, 1306, 1711, 1951
MabI	ACCWGGT	1 cut(s)	1300
MaeI	CTAG	4 cut(s)	18, 1571, 1685, 1778
MaeII	ACGT	2 cut(s)	10, 1896
MaeIII	GTNAC	7 cut(s)	962, 1286, 1469, 1594, 1694, 1773, 1922
MalI	GATC	8 cut(s)	318, 369, 705, 721, 956, 1177, 1530, 1683
MboI	GATC	8 cut(s)	316, 367, 703, 719, 954, 1175, 1528, 1681
MboII	GAAGA	8 cut(s)	83, 177, 386, 467, 807, 869, 1894, 1925
MfeI	CAATTG	1 cut(s)	1281
MflI	RGATCY	2 cut(s)	954, 1175
MhlI	GDGCHC	8 cut(s)	30, 151, 290, 428, 622, 1233, 1570, 1611
MlyI	GAGTC	2 cut(s)	501, 564
MmeI	TCCRAC	2 cut(s)	547, 772
Mph1103I	ATGCAT	1 cut(s)	1431
MroXI	GAANNNNTTC	1 cut(s)	447
MseI	TTAA	4 cut(s)	258, 816, 1038, 1349
MslI	CAYNNNNRTG	5 cut(s)	635, 908, 936, 1236, 1701
MspA1I	CMGCKG	2 cut(s)	1679, 1823
MspI	CCGG	3 cut(s)	158, 222, 1750
MunI	CAATTG	1 cut(s)	1281
MvaI	CCWGG	8 cut(s)	105, 112, 119, 500, 1302, 1413, 1521, 1714
MvnI	CGCG	1 cut(s)	562
NciI	CCSGG	2 cut(s)	222, 223
NdeI	CATATG	1 cut(s)	1702
NdeII	GATC	8 cut(s)	316, 367, 703, 719, 954, 1175, 1528, 1681
NlaIV	GGNNCC	9 cut(s)	109, 116, 780, 956, 985, 1177, 1306, 1576, 1831
NmeAIII	GCCGAG	1 cut(s)	1102
NmuCI	GTSAC	3 cut(s)	1286, 1694, 1773
NsiI	ATGCAT	1 cut(s)	1431
NspI	RCATGY	1 cut(s)	1421
NspV	TTCGAA	1 cut(s)	405
PagI	TCATGA	2 cut(s)	451, 1398
PaqCI	CACCTGC	1 cut(s)	13
PciI	ACATGT	1 cut(s)	1417
PciSI	GCTCTTC	1 cut(s)	1908
PdmI	GAANNNNTTC	1 cut(s)	447
PfeI	GAWTC	1 cut(s)	1948
PflMI	CCANNNNNTGG	1 cut(s)	1826
PfoI	TCCNGGA	2 cut(s)	117, 1519
PinAI	ACCGGT	1 cut(s)	157
PkrI	GCNGC	6 cut(s)	193, 1555, 1669, 1678, 1987, 2065
PleI	GAGTC	2 cut(s)	500, 564
PpsI	GAGTC	2 cut(s)	500, 564
PpuMI	RGGWCCY	1 cut(s)	1409
PscI	ACATGT	1 cut(s)	1417
PsiI	TTATAA	1 cut(s)	662
Psp124BI	GAGCTC	1 cut(s)	290
Psp5II	RGGWCCY	1 cut(s)	1409
Psp6I	CCWGG	8 cut(s)	103, 110, 117, 498, 1300, 1411, 1519, 1712
PspEI	GGTNACC	1 cut(s)	1694
PspFI	CCCAGC	3 cut(s)	322, 503, 1109
PspGI	CCWGG	8 cut(s)	103, 110, 117, 498, 1300, 1411, 1519, 1712
PspN4I	GGNNCC	9 cut(s)	109, 116, 780, 956, 985, 1177, 1306, 1576, 1831
PspPPI	RGGWCCY	1 cut(s)	1409
PstI	CTGCAG	1 cut(s)	193
PsuI	RGATCY	2 cut(s)	954, 1175
PvuII	CAGCTG	1 cut(s)	1823
RsaI	GTAC	5 cut(s)	641, 680, 990, 1127, 1907
RsaNI	GTAC	5 cut(s)	640, 679, 989, 1126, 1906
RseI	CAYNNNNRTG	5 cut(s)	635, 908, 936, 1236, 1701
SacI	GAGCTC	1 cut(s)	290
SapI	GCTCTTC	1 cut(s)	1908
SaqAI	TTAA	4 cut(s)	258, 816, 1038, 1349
SatI	GCNGC	6 cut(s)	192, 1554, 1668, 1677, 1986, 2064
Sau3AI	GATC	8 cut(s)	316, 367, 703, 719, 954, 1175, 1528, 1681
SchI	GAGTC	2 cut(s)	501, 564
SduI	GDGCHC	8 cut(s)	30, 151, 290, 428, 622, 1233, 1570, 1611
SexAI	ACCWGGT	1 cut(s)	1300
SfaNI	GCATC	5 cut(s)	143, 220, 1306, 1711, 1951
SfcI	CTRYAG	2 cut(s)	189, 921
SfuI	TTCGAA	1 cut(s)	405
SinI	GGWCC	9 cut(s)	542, 874, 913, 984, 1409, 1496, 1759, 1862, 2073
SmaI	CCCGGG	1 cut(s)	223
SmiMI	CAYNNNNRTG	5 cut(s)	635, 908, 936, 1236, 1701
SmlI	CTYRAG	1 cut(s)	1707
SmoI	CTYRAG	1 cut(s)	1707
SsiI	CCGC	8 cut(s)	372, 560, 583, 739, 1578, 1677, 1860, 2064
SspMI	CTAG	4 cut(s)	18, 1571, 1685, 1778
SstI	GAGCTC	1 cut(s)	290
StyI	CCWWGG	4 cut(s)	17, 353, 1612, 1833
TaaI	ACNGT	8 cut(s)	76, 364, 907, 988, 1125, 1335, 1628, 1999
TaiI	ACGT	2 cut(s)	13, 1899
TaqI	TCGA	3 cut(s)	405, 487, 855
TatI	WGTACW	2 cut(s)	639, 678
TauI	GCSGC	2 cut(s)	1679, 2066
TfiI	GAWTC	1 cut(s)	1948
Tru1I	TTAA	4 cut(s)	258, 816, 1038, 1349
Tru9I	TTAA	4 cut(s)	258, 816, 1038, 1349
TscAI	CASTG	6 cut(s)	544, 910, 1238, 1333, 1731, 2008
TseFI	GTSAC	3 cut(s)	1286, 1694, 1773
TseI	GCWGC	4 cut(s)	191, 1553, 1667, 1985
Tsp45I	GTSAC	3 cut(s)	1286, 1694, 1773
TspDTI	ATGAA	9 cut(s)	440, 468, 525, 1172, 1415, 1419, 1463, 1824, 1895
TspGWI	ACGGA	4 cut(s)	40, 243, 1035, 1904
TspMI	CCCGGG	1 cut(s)	221
TspRI	CASTG	6 cut(s)	544, 910, 1238, 1333, 1731, 2008
Van91I	CCANNNNNTGG	1 cut(s)	1826
VneI	GTGCAC	1 cut(s)	618
VpaK11BI	GGWCC	9 cut(s)	542, 874, 913, 984, 1409, 1496, 1759, 1862, 2073
XapI	RAATTY	6 cut(s)	44, 800, 832, 1216, 1434, 2034
XceI	RCATGY	1 cut(s)	1421
XmaI	CCCGGG	1 cut(s)	221
XmaJI	CCTAGG	1 cut(s)	17
XmnI	GAANNNNTTC	1 cut(s)	447
XspI	CTAG	4 cut(s)	18, 1571, 1685, 1778
Zsp2I	ATGCAT	1 cut(s)	1431

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.