MD03G1083100.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0009807
Physical Location & Seq
Reverse (-)
6731544 .. 6734703
3160 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1083100.v1.1.491

Sequence Viewer

Length: 486 bp
ATGGAGAGTAACAATAAGTATCAAGGCAATTCAGGTTCCTCATTGACTGCTGATCTTTTTGGTGCCAAGGACAAGGAGTCTCCCCCCAAGTCTTCTACTGGGATTTTTGCTTCCATTTTCCCACCTCCATCACAGGTGGTTGGGAGGAACTCTTCAAGCTCTGAGCTGACAGAATATTGGCAGAAGCAGTCGTCCTTGGGGAATCATGCATGGAACTCCAAGCAAGCTATAAGTGGCGAAGGTGCACACTATAGCTTACCCAACAAGGACCGGAGTTCAGTTCTTCAGGAAGAAAGAGCAGAACCATGTCATCTAAGTTCATCTATTTACTATGGTGGGCAAGAGGTGTATTCCCAGTCCCCAAGTACCCGTTCCTCTGGATCCTATCCTATATTCAAGAAAGATGGGGGAGAAGATGATCCAAATGGAACCAACTCGAACAGTGCTGCCCGAGGAAATTGGTGGCAAGGTTCGCTCTATTATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

162

Amino Acids

17.47

Weight (kDa)

5.9

Isoelectric Point (pI)

64.38

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0009807)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G46880 AT3G46880 AT5G59080 AT5G59080
fragaria_vesca FvH4_3g37190
malus_domestica MD03G1083100.v1.1 MD11G1091200.v1.1
prunus_persica Prupe.6G067400_v2.0.a1 Prupe.6G067400_v2.0.a1
pyrus_communis pycom03g06610 pycom11g07580
rosa_chinensis RchiOBHm_Chr5g0066931
rosa_laevigata RLG00000035894
rosa_multiflora Rmu_sc0008378.1_g000008
rosa_roxburghii Rroxscaffold_1G00014190
rosa_rugosa Rorug05G0379900 Rorug05G0379900
rosa_samantha Rh5BG456500 Rh5CG478600 Rh5DG471300
rosa_wichuraiana Rw5G041100

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 62
AclWI GGATC 3 cut(s) 375, 388, 413
AcuI CTGAAG 1 cut(s) 269
AfaI GTAC 1 cut(s) 367
AgsI TTSAA 2 cut(s) 156, 397
AhdI GACNNNNNGTC 1 cut(s) 76
AjuI GAANNNNNNNTTGG 2 cut(s) 355, 387
AluBI AGCT 4 cut(s) 159, 166, 227, 255
AluI AGCT 4 cut(s) 159, 166, 227, 255
Alw21I GWGCWC 1 cut(s) 247
Alw26I GTCTC 1 cut(s) 84
Alw44I GTGCAC 1 cut(s) 243
AlwI GGATC 3 cut(s) 375, 388, 413
Ama87I CYCGRG 1 cut(s) 450
ApaLI GTGCAC 1 cut(s) 243
ApeKI GCWGC 1 cut(s) 446
AspS9I GGNCC 1 cut(s) 268
AvaI CYCGRG 1 cut(s) 450
AvaII GGWCC 1 cut(s) 268
BaeGI GKGCMC 1 cut(s) 247
BamHI GGATCC 1 cut(s) 380
BanI GGYRCC 1 cut(s) 62
BbsI GAAGAC 1 cut(s) 84
Bbv12I GWGCWC 1 cut(s) 247
BbvI GCAGC 1 cut(s) 433
BccI CCATC 2 cut(s) 136, 398
BcoDI GTCTC 1 cut(s) 84
BfmI CTRYAG 1 cut(s) 250
BisI GCNGC 1 cut(s) 447
BlsI GCNGC 1 cut(s) 448
Bme18I GGWCC 1 cut(s) 268
BmeRI GACNNNNNGTC 1 cut(s) 76
BmeT110I CYCGRG 1 cut(s) 450
BmgT120I GGNCC 1 cut(s) 268
BmiI GGNNCC 4 cut(s) 37, 64, 382, 430
BmrI ACTGGG 2 cut(s) 108, 349
BmuI ACTGGG 2 cut(s) 108, 349
BpiI GAAGAC 1 cut(s) 84
BsaJI CCNNGG 3 cut(s) 66, 195, 451
BsaWI WCCGGW 1 cut(s) 270
Bse1I ACTGG 2 cut(s) 103, 355
BseDI CCNNGG 3 cut(s) 66, 195, 451
BseMII CTCAG 1 cut(s) 153
BseNI ACTGG 2 cut(s) 103, 355
BseSI GKGCMC 1 cut(s) 247
BseXI GCAGC 1 cut(s) 433
BshNI GGYRCC 1 cut(s) 62
BsiHKAI GWGCWC 1 cut(s) 247
BsiHKCI CYCGRG 1 cut(s) 450
BsiSI CCGG 1 cut(s) 271
BslFI GGGAC 1 cut(s) 343
BsmAI GTCTC 1 cut(s) 84
BsmFI GGGAC 1 cut(s) 343
BsoBI CYCGRG 1 cut(s) 450
Bsp1286I GDGCHC 1 cut(s) 247
Bsp143I GATC 3 cut(s) 52, 380, 418
BspCNI CTCAG 1 cut(s) 154
BspLI GGNNCC 4 cut(s) 37, 64, 382, 430
BspPI GGATC 3 cut(s) 375, 388, 413
BspT107I GGYRCC 1 cut(s) 62
BsrI ACTGG 2 cut(s) 103, 355
BssECI CCNNGG 3 cut(s) 66, 195, 451
BssMI GATC 3 cut(s) 52, 380, 418
BssT1I CCWWGG 2 cut(s) 66, 195
Bst4CI ACNGT 1 cut(s) 443
Bst6I CTCTTC 1 cut(s) 157
BstC8I GCNNGC 1 cut(s) 225
BstDEI CTNAG 2 cut(s) 162, 314
BstKTI GATC 3 cut(s) 55, 383, 421
BstMAI GTCTC 1 cut(s) 84
BstMBI GATC 3 cut(s) 52, 380, 418
BstMWI GCNNNNNNNGC 1 cut(s) 472
BstSFI CTRYAG 1 cut(s) 250
BstSLI GKGCMC 1 cut(s) 247
BstV1I GCAGC 1 cut(s) 433
BstV2I GAAGAC 1 cut(s) 84
BstX2I RGATCY 1 cut(s) 380
BstYI RGATCY 1 cut(s) 380
BtsIMutI CAGTG 1 cut(s) 448
Cac8I GCNNGC 1 cut(s) 225
Cfr13I GGNCC 1 cut(s) 268
Csp6I GTAC 1 cut(s) 366
CviAII CATG 3 cut(s) 206, 210, 306
CviJI RGCY 4 cut(s) 159, 166, 227, 255
CviKI_1 RGCY 4 cut(s) 159, 166, 227, 255
CviQI GTAC 1 cut(s) 366
DdeI CTNAG 2 cut(s) 162, 314
DpnI GATC 3 cut(s) 54, 382, 420
DpnII GATC 3 cut(s) 52, 380, 418
DriI GACNNNNNGTC 1 cut(s) 76
Eam1104I CTCTTC 1 cut(s) 157
Eam1105I GACNNNNNGTC 1 cut(s) 76
EarI CTCTTC 1 cut(s) 157
Eco130I CCWWGG 2 cut(s) 66, 195
Eco47I GGWCC 1 cut(s) 268
Eco57I CTGAAG 1 cut(s) 269
Eco88I CYCGRG 1 cut(s) 450
EcoT14I CCWWGG 2 cut(s) 66, 195
EcoT22I ATGCAT 1 cut(s) 211
ErhI CCWWGG 2 cut(s) 66, 195
FaeI CATG 3 cut(s) 209, 213, 309
FaiI YATR 7 cut(s) 207, 211, 230, 252, 307, 333, 392
FaqI GGGAC 1 cut(s) 343
FatI CATG 3 cut(s) 205, 209, 305
Fnu4HI GCNGC 1 cut(s) 447
Fsp4HI GCNGC 1 cut(s) 447
GluI GCNGC 1 cut(s) 447
HapII CCGG 1 cut(s) 271
Hin1II CATG 3 cut(s) 209, 213, 309
HinfI GANTC 2 cut(s) 77, 202
HpaII CCGG 1 cut(s) 271
Hpy166II GTNNAC 1 cut(s) 245
Hpy188I TCNGA 1 cut(s) 163
Hpy188III TCNNGA 3 cut(s) 287, 378, 397
Hpy8I GTNNAC 1 cut(s) 245
HpyAV CCTTC 1 cut(s) 233
HpyCH4III ACNGT 1 cut(s) 443
HpyCH4V TGCA 2 cut(s) 209, 245
HpyF10VI GCNNNNNNNGC 1 cut(s) 472
HpyF3I CTNAG 2 cut(s) 162, 314
Hsp92II CATG 3 cut(s) 209, 213, 309
Kzo9I GATC 3 cut(s) 52, 380, 418
LpnPI CCDG 7 cut(s) 18, 84, 119, 272, 284, 363, 368
Lsp1109I GCAGC 1 cut(s) 433
MaeIII GTNAC 1 cut(s) 8
MalI GATC 3 cut(s) 54, 382, 420
MboI GATC 3 cut(s) 52, 380, 418
MboII GAAGA 5 cut(s) 84, 144, 275, 302, 425
MflI RGATCY 1 cut(s) 380
MhlI GDGCHC 1 cut(s) 247
MluCI AATT 2 cut(s) 28, 457
MlyI GAGTC 1 cut(s) 86
MnlI CCTC 6 cut(s) 49, 135, 138, 337, 385, 446
Mph1103I ATGCAT 1 cut(s) 211
MspI CCGG 1 cut(s) 271
MwoI GCNNNNNNNGC 1 cut(s) 472
NdeII GATC 3 cut(s) 52, 380, 418
NlaIII CATG 3 cut(s) 209, 213, 309
NlaIV GGNNCC 4 cut(s) 37, 64, 382, 430
NsiI ATGCAT 1 cut(s) 211
PfeI GAWTC 1 cut(s) 202
PkrI GCNGC 1 cut(s) 448
PleI GAGTC 1 cut(s) 85
PpsI GAGTC 1 cut(s) 85
PspN4I GGNNCC 4 cut(s) 37, 64, 382, 430
PspPI GGNCC 1 cut(s) 268
PsuI RGATCY 1 cut(s) 380
RsaI GTAC 1 cut(s) 367
RsaNI GTAC 1 cut(s) 366
SatI GCNGC 1 cut(s) 447
Sau3AI GATC 3 cut(s) 52, 380, 418
Sau96I GGNCC 1 cut(s) 268
SchI GAGTC 1 cut(s) 86
SduI GDGCHC 1 cut(s) 247
SfcI CTRYAG 1 cut(s) 250
SinI GGWCC 1 cut(s) 268
Sse9I AATT 2 cut(s) 28, 457
SspI AATATT 1 cut(s) 176
StyI CCWWGG 2 cut(s) 66, 195
TaaI ACNGT 1 cut(s) 443
TaqI TCGA 1 cut(s) 437
TasI AATT 2 cut(s) 28, 457
TfiI GAWTC 1 cut(s) 202
TscAI CASTG 1 cut(s) 448
TseI GCWGC 1 cut(s) 446
TspDTI ATGAA 1 cut(s) 309
TspRI CASTG 1 cut(s) 448
VneI GTGCAC 1 cut(s) 243
VpaK11BI GGWCC 1 cut(s) 268
Zsp2I ATGCAT 1 cut(s) 211
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.