MD03G1090100.v1.1

protein C20orf24 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0015391
Physical Location & Seq
Forward (+)
7426178 .. 7428660
2483 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1090100.v1.1.491

Sequence Viewer

Length: 396 bp
ATGAAAGAAGGTAAATCAGTGAAATTGAACGGTCAACAGCAGCAGTCGCAGCAGCACCAGAACGGTCACTTGTCGCCGTTCAAATTCGCCAAGTTGTTGGATCCGGAAGCTACCTGGGACAAGGATCAATTGGGAGATGTCTTGCATTGGATTCGACAAGCAATGGCCCTCGCATGTGGGCTGCTGTGGGGTGCAATACCGTTGGTCGGAGGTATATGGTTCGTCATTTTTCTGGTGATATCAACTGGAATTATATATGGCTACTATGCAATGATACTAAAGGTTGATGAAGAAGACTTTGGTGGTCATGGAGCTCTTCTCCAAGAGGGGCTTTTCGCTTCTATAACTCTTTTTCTGCTTGCCTGGGTTCTAGTATACAGCTTGGGACACTTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Rab5ip

Protein Analysis

132

Amino Acids

14.62

Weight (kDa)

5.88

Isoelectric Point (pI)

28.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EMC6 PF07019 47 - 126 1.5e-17 EMC6
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015391)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G59410
fragaria_vesca FvH4_3g36490
malus_domestica MD03G1090100.v1.1 MD11G1099700.v1.1
prunus_persica Prupe.6G074300_v2.0.a1
pyrus_communis pycom03g07170
rosa_chinensis RchiOBHm_Chr5g0065351
rosa_laevigata RLG00000035785
rosa_multiflora Rmu_sc0009942.1_g000004
rosa_roxburghii Rroxscaffold_1G00015840
rosa_rugosa Rorug05G0371400
rosa_samantha Rh5AG429300 Rh5BG444600 Rh5CG466900 Rh5DG456900
rosa_wichuraiana Rw5G040300

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 375
AccIII TCCGGA 1 cut(s) 103
AclWI GGATC 3 cut(s) 95, 108, 132
AcsI RAATTY 1 cut(s) 83
AfiI CCNNNNNNNGG 1 cut(s) 206
AgsI TTSAA 2 cut(s) 28, 82
AjnI CCWGG 2 cut(s) 113, 362
AjuI GAANNNNNNNTTGG 2 cut(s) 282, 314
AluBI AGCT 3 cut(s) 110, 314, 381
AluI AGCT 3 cut(s) 110, 314, 381
Alw21I GWGCWC 1 cut(s) 316
AlwI GGATC 3 cut(s) 95, 108, 132
Aor13HI TCCGGA 1 cut(s) 103
AoxI GGCC 1 cut(s) 165
ApeKI GCWGC 4 cut(s) 40, 49, 52, 181
ApoI RAATTY 1 cut(s) 83
AspS9I GGNCC 1 cut(s) 166
AsuHPI GGTGA 1 cut(s) 247
BamHI GGATCC 1 cut(s) 100
BanII GRGCYC 1 cut(s) 316
BbsI GAAGAC 1 cut(s) 300
Bbv12I GWGCWC 1 cut(s) 316
BbvI GCAGC 4 cut(s) 52, 61, 64, 168
BceAI ACGGC 1 cut(s) 61
BcgI CGANNNNNNTGC 2 cut(s) 134, 168
BciT130I CCWGG 2 cut(s) 115, 364
BfaI CTAG 1 cut(s) 371
BisI GCNGC 4 cut(s) 41, 50, 53, 182
BlsI GCNGC 4 cut(s) 42, 51, 54, 183
Bme1390I CCNGG 2 cut(s) 115, 364
BmgT120I GGNCC 1 cut(s) 166
BmiI GGNNCC 1 cut(s) 102
BmrFI CCNGG 2 cut(s) 115, 364
BpiI GAAGAC 1 cut(s) 300
BplI GAGNNNNNCTC 2 cut(s) 303, 335
BsaJI CCNNGG 2 cut(s) 114, 363
BsaWI WCCGGW 1 cut(s) 103
Bsc4I CCNNNNNNNGG 1 cut(s) 206
Bse1I ACTGG 1 cut(s) 250
Bse3DI GCAATG 2 cut(s) 168, 276
BseAI TCCGGA 1 cut(s) 103
BseBI CCWGG 2 cut(s) 115, 364
BseDI CCNNGG 2 cut(s) 114, 363
BseLI CCNNNNNNNGG 1 cut(s) 206
BseMI GCAATG 2 cut(s) 168, 276
BseNI ACTGG 1 cut(s) 250
BseXI GCAGC 4 cut(s) 52, 61, 64, 168
BshFI GGCC 1 cut(s) 167
BsiHKAI GWGCWC 1 cut(s) 316
BsiSI CCGG 1 cut(s) 104
BslFI GGGAC 1 cut(s) 131
BslI CCNNNNNNNGG 1 cut(s) 206
BsmFI GGGAC 1 cut(s) 131
BsnI GGCC 1 cut(s) 167
Bsp1286I GDGCHC 1 cut(s) 316
Bsp13I TCCGGA 1 cut(s) 103
Bsp143I GATC 2 cut(s) 100, 124
BspANI GGCC 1 cut(s) 167
BspEI TCCGGA 1 cut(s) 103
BspLI GGNNCC 1 cut(s) 102
BspPI GGATC 3 cut(s) 95, 108, 132
BspQI GCTCTTC 1 cut(s) 321
BsrDI GCAATG 2 cut(s) 168, 276
BsrI ACTGG 1 cut(s) 250
BssECI CCNNGG 2 cut(s) 114, 363
BssMI GATC 2 cut(s) 100, 124
BssNAI GTATAC 1 cut(s) 376
Bst1107I GTATAC 1 cut(s) 376
Bst2UI CCWGG 2 cut(s) 115, 364
Bst4CI ACNGT 3 cut(s) 32, 65, 201
Bst6I CTCTTC 1 cut(s) 321
BstC8I GCNNGC 1 cut(s) 360
BstKTI GATC 2 cut(s) 103, 127
BstMBI GATC 2 cut(s) 100, 124
BstMWI GCNNNNNNNGC 2 cut(s) 46, 49
BstNI CCWGG 2 cut(s) 115, 364
BstNSI RCATGY 1 cut(s) 177
BstSCI CCNGG 2 cut(s) 113, 362
BstV1I GCAGC 4 cut(s) 52, 61, 64, 168
BstV2I GAAGAC 1 cut(s) 300
BstX2I RGATCY 1 cut(s) 100
BstXI CCANNNNNNTGG 1 cut(s) 97
BstYI RGATCY 1 cut(s) 100
BstZ17I GTATAC 1 cut(s) 376
BsuRI GGCC 1 cut(s) 167
BtsIMutI CAGTG 1 cut(s) 24
Cac8I GCNNGC 1 cut(s) 360
Cfr13I GGNCC 1 cut(s) 166
CviAII CATG 2 cut(s) 174, 308
CviJI RGCY 7 cut(s) 110, 167, 181, 261, 314, 331, 381
CviKI_1 RGCY 7 cut(s) 110, 167, 181, 261, 314, 331, 381
DpnI GATC 2 cut(s) 102, 126
DpnII GATC 2 cut(s) 100, 124
Eam1104I CTCTTC 1 cut(s) 321
EarI CTCTTC 1 cut(s) 321
Ecl136II GAGCTC 1 cut(s) 314
Eco24I GRGCYC 1 cut(s) 316
Eco32I GATATC 1 cut(s) 240
Eco53kI GAGCTC 1 cut(s) 314
EcoICRI GAGCTC 1 cut(s) 314
EcoRII CCWGG 2 cut(s) 113, 362
EcoRV GATATC 1 cut(s) 240
EcoT38I GRGCYC 1 cut(s) 316
FaeI CATG 2 cut(s) 177, 311
FalI AAGNNNNNCTT 2 cut(s) 315, 347
FaqI GGGAC 1 cut(s) 131
FatI CATG 2 cut(s) 173, 307
FblI GTMKAC 1 cut(s) 375
Fnu4HI GCNGC 4 cut(s) 41, 50, 53, 182
FriOI GRGCYC 1 cut(s) 316
Fsp4HI GCNGC 4 cut(s) 41, 50, 53, 182
FspBI CTAG 1 cut(s) 371
GluI GCNGC 4 cut(s) 41, 50, 53, 182
HaeIII GGCC 1 cut(s) 167
HapII CCGG 1 cut(s) 104
Hin1II CATG 2 cut(s) 177, 311
HincII GTYRAC 1 cut(s) 35
HindII GTYRAC 1 cut(s) 35
HinfI GANTC 1 cut(s) 151
HpaII CCGG 1 cut(s) 104
HphI GGTGA 1 cut(s) 247
Hpy166II GTNNAC 2 cut(s) 35, 376
Hpy188I TCNGA 2 cut(s) 209, 395
Hpy188III TCNNGA 1 cut(s) 104
Hpy8I GTNNAC 2 cut(s) 35, 376
HpyCH4III ACNGT 3 cut(s) 32, 65, 201
HpyCH4V TGCA 3 cut(s) 145, 194, 269
HpyF10VI GCNNNNNNNGC 2 cut(s) 46, 49
Hsp92II CATG 2 cut(s) 177, 311
Kpn2I TCCGGA 1 cut(s) 103
Kzo9I GATC 2 cut(s) 100, 124
LguI GCTCTTC 1 cut(s) 321
LmnI GCTCC 1 cut(s) 311
LpnPI CCDG 8 cut(s) 71, 100, 117, 127, 218, 231, 349, 376
Lsp1109I GCAGC 4 cut(s) 52, 61, 64, 168
MaeI CTAG 1 cut(s) 371
MaeIII GTNAC 1 cut(s) 65
MalI GATC 2 cut(s) 102, 126
MboI GATC 2 cut(s) 100, 124
MboII GAAGA 3 cut(s) 302, 305, 308
MfeI CAATTG 1 cut(s) 128
MflI RGATCY 1 cut(s) 100
MhlI GDGCHC 1 cut(s) 316
MluCI AATT 4 cut(s) 23, 83, 128, 249
MmeI TCCRAC 2 cut(s) 78, 187
MnlI CCTC 3 cut(s) 179, 203, 319
MroI TCCGGA 1 cut(s) 103
MspI CCGG 1 cut(s) 104
MspR9I CCNGG 2 cut(s) 115, 364
MunI CAATTG 1 cut(s) 128
MvaI CCWGG 2 cut(s) 115, 364
MwoI GCNNNNNNNGC 2 cut(s) 46, 49
NdeII GATC 2 cut(s) 100, 124
NlaIII CATG 2 cut(s) 177, 311
NlaIV GGNNCC 1 cut(s) 102
NmuCI GTSAC 1 cut(s) 65
NspI RCATGY 1 cut(s) 177
PciSI GCTCTTC 1 cut(s) 321
PfeI GAWTC 1 cut(s) 151
PkrI GCNGC 4 cut(s) 42, 51, 54, 183
Psp124BI GAGCTC 1 cut(s) 316
Psp6I CCWGG 2 cut(s) 113, 362
PspGI CCWGG 2 cut(s) 113, 362
PspN4I GGNNCC 1 cut(s) 102
PspPI GGNCC 1 cut(s) 166
PsuI RGATCY 1 cut(s) 100
SacI GAGCTC 1 cut(s) 316
SapI GCTCTTC 1 cut(s) 321
SatI GCNGC 4 cut(s) 41, 50, 53, 182
Sau3AI GATC 2 cut(s) 100, 124
Sau96I GGNCC 1 cut(s) 166
ScrFI CCNGG 2 cut(s) 115, 364
SduI GDGCHC 1 cut(s) 316
SetI ASST 7 cut(s) 13, 112, 116, 214, 285, 316, 383
Sse9I AATT 4 cut(s) 23, 83, 128, 249
SspMI CTAG 1 cut(s) 371
SstI GAGCTC 1 cut(s) 316
StyD4I CCNGG 2 cut(s) 113, 362
TaaI ACNGT 3 cut(s) 32, 65, 201
TaqI TCGA 1 cut(s) 154
TasI AATT 4 cut(s) 23, 83, 128, 249
TfiI GAWTC 1 cut(s) 151
TscAI CASTG 1 cut(s) 24
TseFI GTSAC 1 cut(s) 65
TseI GCWGC 4 cut(s) 40, 49, 52, 181
Tsp45I GTSAC 1 cut(s) 65
TspDTI ATGAA 2 cut(s) 17, 303
TspRI CASTG 1 cut(s) 24
XapI RAATTY 1 cut(s) 83
XceI RCATGY 1 cut(s) 177
XmiI GTMKAC 1 cut(s) 375
XspI CTAG 1 cut(s) 371
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.