Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr03

Orthogroup ID

OG0013053

Physical Location & Seq

Genomic Coordinates Reverse (-)

7773721 .. 7775424

Gene Structure 1704 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD03G1092900.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1704 bp

ATGTCGCTAACGAGGGAAGCTCAGCTTCTGATTCAAAAAATGGTTAAAAGCCCGCCACTAAAAGCCTTCTCAATCTTCAACTCCTCCACCCTCCGCGGCTTCCATCACTCCCATCAATCCATTTCCTTCATTCTCCACCACCTCCTCGCCTCCAACTTGATCTCCCACGCCCAGTCCCTCATCCTCCAAGTCCTCTCCGGCCGCCTTTCCTCACCGTTCTTCACTCCTTCCTCCCTCCTCGGCCACTTAACTCAGCCCAATCTGAATCCAACCTCCGTCTGCGGCAATCTATACGAAGCAATAGTCAATGCCCATGCTCAATCTCAGTCTCCGGAGCAAGCCCTTTACTTTTTGAAGCAGATGGTTGAGCAGGGCCTCGTTCCCAGATCGAACACTTTCAATAATCTGTTGGGGTCTCTCGTGAAGGCGAAAGATCACGGAAAAGCGTGGCGGGTTTTCGATGAGTTTAAGGGCAAAGTTGAATTGGATGTGTATAGTTTTGGGATAGTGATGAAAAGTTGCTGCGAGGCCGGCAATTTGGATCGGGGATTTGAGCTTTTGATTGAATTAGAGGAATTAGGTTGGGTGCCGAATGTTGTTTTGTACACTACATTGATCGATGGATGCTGCAAAAATGGCGATTTGGAGCGTGCCAAGAAGATGTTTTCAAAAATAGAGGAGGTTGGTTTGGTTGCCAACCAGTATACTTACACTGTTTTGATTCATGGGTTTTTCAAGAAAGGGTATAAAAAAGATGGGTTCGAGCTCTACGAGAAGATGAAAAGTAATGGGGTTGTTCCGAATGTGTGTACTTATACTTGTTTGATCAATGAGTGTTGCAGTAATGGGGAAGTGAAGAGAGCCCTGAAACTGTTCGACGAAATGCGTGAAAGAGGGGTGGCTCGCAATGTGGTAGCGTACAACACCGTGATCGGTGGGTTGTGCAAGGAAACAAGGATTTGGGAAGCTGATAACTTTGTGAATCGGATGAAGAGGGAGGGTATTAGTCCGAATGTAGTTACGTACAATGCGCTGATTGAGGGGTTTTGCAACGTCGGAGAGTTGGACAGGGCTTCGAGTTTATTTAATCAGTTGAAGTCAAGTGGTCTGTCTCCAACTTTGGTGACTTACAATGTTTTGATTCAAGGCTTTGCTAGAGCACAAAATTCTGCAAGAGCTTCTGATTTGGTGAGAGAGATGAGCGACAGAGGCGTTTCTCCTTCGAAAGTGACATACACAATCTTGATCGATGACTTGGTTCGATCTGGTTACACGGAAAGAGCATTTCAGATATTCTCCTCCATGGAGAAGGCCGGAGTAGTGCCTGATGTTTACACCTACGGTGTCTTGATTCATGGGTTATGCACGAAAGGTGATATGAAGGAAGCATCGAAGCTGTTCAAATCAATGGGTGACAAGCATTTGAACCCAAATGATGTCGTATTCAATATGATGATTCATGGTTACTGCAGAGAGGGTAGCTCTTACAGGGCACTGAGGCTGCTCAAAGAGATGAGAAGGAACGGAATGATTCCAAATGTGGCCAGCTACAGTTCGACCATTGGAGTTCTCTGTAATGATGGGAAGTGGGAGGATGCCGAAATGCTGCTCAAGGACATGACAGAGTCAGGTTTAAAACCACCAGTTTCTTTGTATAATGTAATATCTCGAGTGAAATTTAATAAGACACTGGTGAAGTTATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000959 GO:0001101 GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0004518 GO:0004519 GO:0005488 GO:0006139 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009451 GO:0009719 GO:0009725 GO:0009737 GO:0009987 GO:0010033 GO:0016070 GO:0016071 GO:0016556 GO:0016787 GO:0016788 GO:0033993 GO:0034641 GO:0042221 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0046483 GO:0050896 GO:0071704 GO:0080156 GO:0090304 GO:0090305 GO:0097159 GO:0097305 GO:1900864 GO:1901360 GO:1901363 GO:1901700

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PPR PPR_1 PPR_2 PPR_3 PPR_long

Protein Analysis

568

Amino Acids

63.38

Weight (kDa)

8.87

Isoelectric Point (pI)

31.97

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
TPR_24	PF23276	98 - 186	9.3e-06	Fungal tetratrico peptide repeats
PPR_2	PF13041	130 - 175	2.2e-07	PPR repeat family
PPR_1	PF12854	196 - 225	4.9e-11	PPR repeat
PPR_2	PF13041	197 - 246	1.3e-15	PPR repeat family
PPR	PF01535	200 - 229	6.6e-08	PPR repeat
PPR_long	PF17177	204 - 317	1.2e-10	Pentacotripeptide-repeat region of PRORP
TPR_24	PF23276	208 - 315	1e-05	Fungal tetratrico peptide repeats
PPR_1	PF12854	229 - 261	9.8e-06	PPR repeat
PPR_3	PF13812	232 - 279	4.7e-10	Pentatricopeptide repeat domain
PPR	PF01535	235 - 265	3.9e-06	PPR repeat
PPR_2	PF13041	236 - 280	8.7e-15	PPR repeat family
PPR_1	PF12854	263 - 296	2.5e-13	PPR repeat
PPR_2	PF13041	267 - 303	2.1e-11	PPR repeat family
PPR	PF01535	271 - 300	5.6e-09	PPR repeat
PPR_3	PF13812	271 - 312	1.8e-06	Pentatricopeptide repeat domain
TPR_24	PF23276	271 - 364	8e-06	Fungal tetratrico peptide repeats
PPR_long	PF17177	274 - 417	5.6e-14	Pentacotripeptide-repeat region of PRORP
PPR_1	PF12854	298 - 331	5e-07	PPR repeat
PPR_2	PF13041	302 - 351	1.2e-17	PPR repeat family
PPR_3	PF13812	328 - 382	1.2e-15	Pentatricopeptide repeat domain
PPR_1	PF12854	334 - 365	2.8e-12	PPR repeat
PPR_2	PF13041	337 - 386	1.4e-17	PPR repeat family
PPR	PF01535	340 - 370	9e-07	PPR repeat
PPR_1	PF12854	369 - 401	4.7e-07	PPR repeat
PPR_2	PF13041	375 - 420	5.6e-13	PPR repeat family
PPR_long	PF17177	392 - 477	2.8e-06	Pentacotripeptide-repeat region of PRORP
PPR_3	PF13812	396 - 451	7.4e-10	Pentatricopeptide repeat domain
PPR_1	PF12854	403 - 436	4e-06	PPR repeat
PPR_2	PF13041	415 - 455	2.8e-11	PPR repeat family
PPR_3	PF13812	435 - 489	7.7e-09	Pentatricopeptide repeat domain
PPR_1	PF12854	439 - 470	2e-11	PPR repeat
PPR_2	PF13041	450 - 480	4.7e-06	PPR repeat family
TPR_24	PF23276	457 - 555	5.4e-06	Fungal tetratrico peptide repeats
PPR_1	PF12854	473 - 506	5e-09	PPR repeat
PPR_2	PF13041	477 - 526	3.6e-13	PPR repeat family
PPR	PF01535	480 - 509	1.4e-07	PPR repeat
PPR_3	PF13812	500 - 555	1e-06	Pentatricopeptide repeat domain
PPR_2	PF13041	512 - 548	1.9e-06	PPR repeat family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

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Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0013053)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G11690 AT4G11690
fragaria_vesca	FvH4_3g36170
malus_domestica	MD03G1092900.v1.1
prunus_persica	Prupe.6G076800_v2.0.a1
pyrus_communis	pycom03g07420
rosa_chinensis	RchiOBHm_Chr5g0064671 RchiOBHm_Chr5g0064911
rosa_laevigata	RLG00000035740 RLG00000035742
rosa_multiflora	Rmu_sc0020903.1_g000003 Rmu_sc0025431.1_g000001
rosa_roxburghii	Rroxscaffold_1G00016400
rosa_rugosa	Rorug05G0366600
rosa_samantha	Rh5AG425100 Rh5CG462600 Rh5DG453100
rosa_wichuraiana	Rw5G039940

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	1 cut(s)	586
AccI	GTMKAC	1 cut(s)	704
AccII	CGCG	1 cut(s)	96
AccIII	TCCGGA	1 cut(s)	331
AciI	CCGC	6 cut(s)	53, 94, 96, 202, 282, 451
AclWI	GGATC	1 cut(s)	549
AcoI	YGGCCR	3 cut(s)	199, 241, 1542
AcsI	RAATTY	2 cut(s)	1165, 1676
AfaI	GTAC	4 cut(s)	605, 811, 920, 1025
AluBI	AGCT	9 cut(s)	20, 25, 556, 766, 968, 1178, 1396, 1482, 1548
AluI	AGCT	9 cut(s)	20, 25, 556, 766, 968, 1178, 1396, 1482, 1548
Alw21I	GWGCWC	2 cut(s)	768, 1162
Alw26I	GTCTC	3 cut(s)	333, 420, 1116
AlwI	GGATC	1 cut(s)	549
AlwNI	CAGNNNCTG	1 cut(s)	28
Ama87I	CYCGRG	1 cut(s)	1668
Aor13HI	TCCGGA	1 cut(s)	331
AoxI	GGCC	6 cut(s)	199, 241, 373, 528, 1311, 1542
ApeKI	GCWGC	4 cut(s)	522, 627, 1501, 1606
ApoI	RAATTY	2 cut(s)	1165, 1676
ArsI	GACNNNNNNTTYG	2 cut(s)	288, 320
Asp700I	GAANNNNTTC	3 cut(s)	395, 872, 1397
AspLEI	GCGC	1 cut(s)	1033
AspS9I	GGNCC	1 cut(s)	373
AsuHPI	GGTGA	5 cut(s)	204, 1135, 1201, 1385, 1424
AsuII	TTCGAA	1 cut(s)	1223
AvaI	CYCGRG	1 cut(s)	1668
BaeGI	GKGCMC	1 cut(s)	1495
BalI	TGGCCA	1 cut(s)	1544
BanI	GGYRCC	1 cut(s)	586
BanII	GRGCYC	2 cut(s)	768, 865
BauI	CACGAG	1 cut(s)	419
Bbv12I	GWGCWC	2 cut(s)	768, 1162
BbvI	GCAGC	4 cut(s)	509, 614, 1488, 1593
BccI	CCATC	6 cut(s)	111, 120, 355, 614, 749, 1574
BclI	TGATCA	1 cut(s)	825
BcoDI	GTCTC	3 cut(s)	333, 420, 1116
BfaI	CTAG	1 cut(s)	1155
BfmI	CTRYAG	2 cut(s)	1468, 1549
BisI	GCNGC	7 cut(s)	97, 202, 283, 523, 628, 1502, 1607
BlpI	GCTNAGC	1 cut(s)	21
BlsI	GCNGC	7 cut(s)	98, 203, 284, 524, 629, 1503, 1608
BmeT110I	CYCGRG	1 cut(s)	1668
BmgT120I	GGNCC	1 cut(s)	373
BmiI	GGNNCC	1 cut(s)	588
BmrI	ACTGGG	1 cut(s)	166
BmsI	GCATC	3 cut(s)	614, 1397, 1585
BmuI	ACTGGG	1 cut(s)	166
BplI	GAGNNNNNCTC	3 cut(s)	36, 1466, 1498
Bpu1102I	GCTNAGC	1 cut(s)	21
Bpu14I	TTCGAA	1 cut(s)	1223
BpuEI	CTTGAG	1 cut(s)	1595
Bsa29I	ATCGAT	2 cut(s)	618, 1248
BsaAI	YACGTR	1 cut(s)	1023
BsaI	GGTCTC	1 cut(s)	420
BsaJI	CCNNGG	3 cut(s)	94, 238, 1302
BsaWI	WCCGGW	1 cut(s)	331
BsaXI	ACNNNNNCTCC	2 cut(s)	146, 176
Bse118I	RCCGGY	1 cut(s)	530
Bse1I	ACTGG	4 cut(s)	172, 700, 1643, 1695
Bse3DI	GCAATG	1 cut(s)	913
BseAI	TCCGGA	1 cut(s)	331
BseCI	ATCGAT	2 cut(s)	618, 1248
BseDI	CCNNGG	3 cut(s)	94, 238, 1302
BseGI	GGATG	5 cut(s)	180, 493, 629, 993, 1600
BseMI	GCAATG	1 cut(s)	913
BseMII	CTCAG	4 cut(s)	35, 266, 338, 1487
BseNI	ACTGG	4 cut(s)	172, 700, 1643, 1695
BseRI	GAGGAG	5 cut(s)	73, 134, 227, 692, 1288
BseSI	GKGCMC	1 cut(s)	1495
BseX3I	CGGCCG	1 cut(s)	199
BseXI	GCAGC	4 cut(s)	509, 614, 1488, 1593
Bsh1236I	CGCG	1 cut(s)	96
Bsh1285I	CGRYCG	1 cut(s)	202
BshFI	GGCC	6 cut(s)	201, 243, 375, 530, 1313, 1544
BshNI	GGYRCC	1 cut(s)	586
BshVI	ATCGAT	2 cut(s)	618, 1248
BsiEI	CGRYCG	1 cut(s)	202
BsiHKAI	GWGCWC	2 cut(s)	768, 1162
BsiHKCI	CYCGRG	1 cut(s)	1668
BsiSI	CCGG	4 cut(s)	198, 332, 531, 1314
BslFI	GGGAC	1 cut(s)	160
BsmAI	GTCTC	3 cut(s)	333, 420, 1116
BsmFI	GGGAC	1 cut(s)	160
BsnI	GGCC	6 cut(s)	201, 243, 375, 530, 1313, 1544
Bso31I	GGTCTC	1 cut(s)	420
BsoBI	CYCGRG	1 cut(s)	1668
Bsp119I	TTCGAA	1 cut(s)	1223
Bsp1286I	GDGCHC	4 cut(s)	768, 865, 1162, 1495
Bsp13I	TCCGGA	1 cut(s)	331
Bsp1407I	TGTACA	1 cut(s)	603
Bsp143I	GATC	9 cut(s)	159, 386, 433, 541, 615, 825, 930, 1245, 1262
Bsp1720I	GCTNAGC	1 cut(s)	21
Bsp19I	CCATGG	1 cut(s)	1302
BspACI	CCGC	6 cut(s)	53, 94, 96, 202, 282, 451
BspANI	GGCC	6 cut(s)	201, 243, 375, 530, 1313, 1544
BspCNI	CTCAG	4 cut(s)	34, 265, 337, 1488
BspDI	ATCGAT	2 cut(s)	618, 1248
BspEI	TCCGGA	1 cut(s)	331
BspFNI	CGCG	1 cut(s)	96
BspLI	GGNNCC	1 cut(s)	588
BspMAI	CTGCAG	1 cut(s)	1472
BspPI	GGATC	1 cut(s)	549
BspT104I	TTCGAA	1 cut(s)	1223
BspT107I	GGYRCC	1 cut(s)	586
BspTNI	GGTCTC	1 cut(s)	420
BsrDI	GCAATG	1 cut(s)	913
BsrFI	RCCGGY	1 cut(s)	530
BsrGI	TGTACA	1 cut(s)	603
BsrI	ACTGG	4 cut(s)	172, 700, 1643, 1695
BssAI	RCCGGY	1 cut(s)	530
BssECI	CCNNGG	3 cut(s)	94, 238, 1302
BssMI	GATC	9 cut(s)	159, 386, 433, 541, 615, 825, 930, 1245, 1262
BssNAI	GTATAC	1 cut(s)	705
BssSI	CACGAG	1 cut(s)	419
BssT1I	CCWWGG	1 cut(s)	1302
Bst1107I	GTATAC	1 cut(s)	705
Bst2BI	CACGAG	1 cut(s)	419
Bst4CI	ACNGT	6 cut(s)	216, 715, 873, 928, 1343, 1553
Bst6I	CTCTTC	2 cut(s)	851, 986
BstAUI	TGTACA	1 cut(s)	603
BstBAI	YACGTR	1 cut(s)	1023
BstBI	TTCGAA	1 cut(s)	1223
BstC8I	GCNNGC	6 cut(s)	53, 339, 532, 651, 904, 1546
BstDEI	CTNAG	4 cut(s)	21, 252, 324, 1496
BstDSI	CCRYGG	2 cut(s)	94, 1302
BstF5I	GGATG	5 cut(s)	180, 493, 629, 993, 1600
BstFNI	CGCG	1 cut(s)	96
BstHHI	GCGC	1 cut(s)	1033
BstKTI	GATC	9 cut(s)	162, 389, 436, 544, 618, 828, 933, 1248, 1265
BstMAI	GTCTC	3 cut(s)	333, 420, 1116
BstMBI	GATC	9 cut(s)	159, 386, 433, 541, 615, 825, 930, 1245, 1262
BstMCI	CGRYCG	1 cut(s)	202
BstMWI	GCNNNNNNNGC	3 cut(s)	531, 636, 1209
BstSFI	CTRYAG	2 cut(s)	1468, 1549
BstSLI	GKGCMC	1 cut(s)	1495
BstSNI	TACGTA	1 cut(s)	1023
BstUI	CGCG	1 cut(s)	96
BstV1I	GCAGC	4 cut(s)	509, 614, 1488, 1593
BstZ17I	GTATAC	1 cut(s)	705
BstZI	CGGCCG	1 cut(s)	199
Bsu15I	ATCGAT	2 cut(s)	618, 1248
BsuRI	GGCC	6 cut(s)	201, 243, 375, 530, 1313, 1544
BsuTUI	ATCGAT	2 cut(s)	618, 1248
BtgI	CCRYGG	2 cut(s)	94, 1302
BtsCI	GGATG	5 cut(s)	180, 493, 629, 993, 1600
BtsIMutI	CAGTG	3 cut(s)	711, 1493, 1688
Cac8I	GCNNGC	6 cut(s)	53, 339, 532, 651, 904, 1546
CaiI	CAGNNNCTG	1 cut(s)	28
CfoI	GCGC	1 cut(s)	1033
Cfr10I	RCCGGY	1 cut(s)	530
Cfr13I	GGNCC	1 cut(s)	373
Cfr42I	CCGCGG	1 cut(s)	97
ClaI	ATCGAT	2 cut(s)	618, 1248
Csp6I	GTAC	4 cut(s)	604, 810, 919, 1024
CviAII	CATG	6 cut(s)	314, 725, 1303, 1355, 1460, 1618
CviQI	GTAC	4 cut(s)	604, 810, 919, 1024
DdeI	CTNAG	4 cut(s)	21, 252, 324, 1496
DpnI	GATC	9 cut(s)	161, 388, 435, 543, 617, 827, 932, 1247, 1264
DpnII	GATC	9 cut(s)	159, 386, 433, 541, 615, 825, 930, 1245, 1262
DraI	TTTAAA	1 cut(s)	1635
EaeI	YGGCCR	3 cut(s)	199, 241, 1542
EagI	CGGCCG	1 cut(s)	199
Eam1104I	CTCTTC	2 cut(s)	851, 986
EarI	CTCTTC	2 cut(s)	851, 986
Ecl136II	GAGCTC	1 cut(s)	766
EclXI	CGGCCG	1 cut(s)	199
Eco105I	TACGTA	1 cut(s)	1023
Eco130I	CCWWGG	1 cut(s)	1302
Eco24I	GRGCYC	2 cut(s)	768, 865
Eco31I	GGTCTC	1 cut(s)	420
Eco52I	CGGCCG	1 cut(s)	199
Eco53kI	GAGCTC	1 cut(s)	766
Eco88I	CYCGRG	1 cut(s)	1668
EcoICRI	GAGCTC	1 cut(s)	766
EcoO109I	RGGNCCY	1 cut(s)	373
EcoT14I	CCWWGG	1 cut(s)	1302
EcoT38I	GRGCYC	2 cut(s)	768, 865
ErhI	CCWWGG	1 cut(s)	1302
FaeI	CATG	6 cut(s)	317, 728, 1306, 1358, 1463, 1621
FalI	AAGNNNNNCTT	2 cut(s)	9, 41
FaqI	GGGAC	1 cut(s)	160
FatI	CATG	6 cut(s)	313, 724, 1302, 1354, 1459, 1617
FauI	CCCGC	2 cut(s)	60, 444
FbaI	TGATCA	1 cut(s)	825
FblI	GTMKAC	1 cut(s)	704
Fnu4HI	GCNGC	7 cut(s)	97, 202, 283, 523, 628, 1502, 1607
FokI	GGATG	5 cut(s)	167, 500, 636, 1000, 1607
FriOI	GRGCYC	2 cut(s)	768, 865
Fsp4HI	GCNGC	7 cut(s)	97, 202, 283, 523, 628, 1502, 1607
FspBI	CTAG	1 cut(s)	1155
GlaI	GCGC	1 cut(s)	1032
GluI	GCNGC	7 cut(s)	97, 202, 283, 523, 628, 1502, 1607
HaeIII	GGCC	6 cut(s)	201, 243, 375, 530, 1313, 1544
HapII	CCGG	4 cut(s)	198, 332, 531, 1314
HhaI	GCGC	1 cut(s)	1033
Hin1II	CATG	6 cut(s)	317, 728, 1306, 1358, 1463, 1621
Hin6I	GCGC	1 cut(s)	1031
HinP1I	GCGC	1 cut(s)	1031
HinfI	GANTC	9 cut(s)	31, 265, 721, 982, 1141, 1351, 1456, 1531, 1625
HpaII	CCGG	4 cut(s)	198, 332, 531, 1314
HphI	GGTGA	5 cut(s)	204, 1135, 1201, 1385, 1424
Hpy166II	GTNNAC	4 cut(s)	606, 705, 810, 1333
Hpy188I	TCNGA	8 cut(s)	30, 264, 801, 987, 1011, 1058, 1183, 1290
Hpy188III	TCNNGA	6 cut(s)	332, 421, 736, 1243, 1348, 1668
Hpy8I	GTNNAC	4 cut(s)	606, 705, 810, 1333
Hpy99I	CGWCG	2 cut(s)	881, 1058
HpyAV	CCTTC	8 cut(s)	76, 136, 237, 418, 1230, 1303, 1375, 1512
HpyCH4III	ACNGT	6 cut(s)	216, 715, 873, 928, 1343, 1553
HpyCH4IV	ACGT	2 cut(s)	1022, 1053
HpyCH4V	TGCA	7 cut(s)	630, 840, 945, 1050, 1172, 1365, 1470
HpyF10VI	GCNNNNNNNGC	3 cut(s)	531, 636, 1209
HpyF3I	CTNAG	4 cut(s)	21, 252, 324, 1496
HpySE526I	ACGT	2 cut(s)	1022, 1053
Hsp92II	CATG	6 cut(s)	317, 728, 1306, 1358, 1463, 1621
HspAI	GCGC	1 cut(s)	1031
Kpn2I	TCCGGA	1 cut(s)	331
KroI	GCCGGC	1 cut(s)	530
KroNI	GCCGGC	1 cut(s)	532
Ksp22I	TGATCA	1 cut(s)	825
KspI	CCGCGG	1 cut(s)	97
Kzo9I	GATC	9 cut(s)	159, 386, 433, 541, 615, 825, 930, 1245, 1262
LmnI	GCTCC	2 cut(s)	334, 646
Lsp1109I	GCAGC	4 cut(s)	509, 614, 1488, 1593
LweI	GCATC	3 cut(s)	614, 1397, 1585
MaeI	CTAG	1 cut(s)	1155
MaeII	ACGT	2 cut(s)	1022, 1053
MaeIII	GTNAC	6 cut(s)	1018, 1123, 1228, 1268, 1412, 1463
MalI	GATC	9 cut(s)	161, 388, 435, 543, 617, 827, 932, 1247, 1264
MboI	GATC	9 cut(s)	159, 386, 433, 541, 615, 825, 930, 1245, 1262
MboII	GAAGA	6 cut(s)	67, 211, 670, 787, 868, 1003
MhlI	GDGCHC	4 cut(s)	768, 865, 1162, 1495
MlsI	TGGCCA	1 cut(s)	1544
MluCI	AATT	6 cut(s)	482, 535, 566, 575, 1165, 1676
MluNI	TGGCCA	1 cut(s)	1544
MlyI	GAGTC	1 cut(s)	1634
MmeI	TCCRAC	5 cut(s)	177, 293, 1036, 1044, 1139
Mox20I	TGGCCA	1 cut(s)	1544
MroI	TCCGGA	1 cut(s)	331
MroNI	GCCGGC	1 cut(s)	530
MroXI	GAANNNNTTC	3 cut(s)	395, 872, 1397
MscI	TGGCCA	1 cut(s)	1544
MseI	TTAA	6 cut(s)	45, 248, 468, 1086, 1634, 1680
Msp20I	TGGCCA	1 cut(s)	1544
MspA1I	CMGCKG	1 cut(s)	96
MspI	CCGG	4 cut(s)	198, 332, 531, 1314
MvnI	CGCG	1 cut(s)	96
MwoI	GCNNNNNNNGC	3 cut(s)	531, 636, 1209
NaeI	GCCGGC	1 cut(s)	532
NcoI	CCATGG	1 cut(s)	1302
NdeII	GATC	9 cut(s)	159, 386, 433, 541, 615, 825, 930, 1245, 1262
NgoMIV	GCCGGC	1 cut(s)	530
NlaIII	CATG	6 cut(s)	317, 728, 1306, 1358, 1463, 1621
NlaIV	GGNNCC	1 cut(s)	588
NmeAIII	GCCGAG	1 cut(s)	219
NmuCI	GTSAC	3 cut(s)	1123, 1228, 1412
NspV	TTCGAA	1 cut(s)	1223
PaeR7I	CTCGAG	1 cut(s)	1668
PcsI	WCGNNNNNNNCGW	1 cut(s)	768
PdiI	GCCGGC	1 cut(s)	532
PdmI	GAANNNNTTC	3 cut(s)	395, 872, 1397
PfeI	GAWTC	8 cut(s)	31, 265, 721, 982, 1141, 1351, 1456, 1531
PflFI	GACNNNGTC	1 cut(s)	1624
PkrI	GCNGC	7 cut(s)	98, 203, 284, 524, 629, 1503, 1608
PleI	GAGTC	1 cut(s)	1633
PpsI	GAGTC	1 cut(s)	1633
Ppu21I	YACGTR	1 cut(s)	1023
Psp124BI	GAGCTC	1 cut(s)	768
PspN4I	GGNNCC	1 cut(s)	588
PspPI	GGNCC	1 cut(s)	373
PstI	CTGCAG	1 cut(s)	1472
PstNI	CAGNNNCTG	1 cut(s)	28
PsyI	GACNNNGTC	1 cut(s)	1624
RsaI	GTAC	4 cut(s)	605, 811, 920, 1025
RsaNI	GTAC	4 cut(s)	604, 810, 919, 1024
SacI	GAGCTC	1 cut(s)	768
SacII	CCGCGG	1 cut(s)	97
SaqAI	TTAA	6 cut(s)	45, 248, 468, 1086, 1634, 1680
SatI	GCNGC	7 cut(s)	97, 202, 283, 523, 628, 1502, 1607
Sau3AI	GATC	9 cut(s)	159, 386, 433, 541, 615, 825, 930, 1245, 1262
Sau96I	GGNCC	1 cut(s)	373
SchI	GAGTC	1 cut(s)	1634
SduI	GDGCHC	4 cut(s)	768, 865, 1162, 1495
SfaNI	GCATC	3 cut(s)	614, 1397, 1585
SfcI	CTRYAG	2 cut(s)	1468, 1549
Sfr274I	CTCGAG	1 cut(s)	1668
Sfr303I	CCGCGG	1 cut(s)	97
SfuI	TTCGAA	1 cut(s)	1223
SgrBI	CCGCGG	1 cut(s)	97
SlaI	CTCGAG	1 cut(s)	1668
SmlI	CTYRAG	2 cut(s)	1610, 1668
SmoI	CTYRAG	2 cut(s)	1610, 1668
SnaBI	TACGTA	1 cut(s)	1023
Sse9I	AATT	6 cut(s)	482, 535, 566, 575, 1165, 1676
SsiI	CCGC	6 cut(s)	53, 94, 96, 202, 282, 451
SspMI	CTAG	1 cut(s)	1155
SstI	GAGCTC	1 cut(s)	768
StyI	CCWWGG	1 cut(s)	1302
TaaI	ACNGT	6 cut(s)	216, 715, 873, 928, 1343, 1553
TaiI	ACGT	2 cut(s)	1025, 1056
TasI	AATT	6 cut(s)	482, 535, 566, 575, 1165, 1676
TatI	WGTACW	2 cut(s)	603, 809
TauI	GCSGC	3 cut(s)	99, 204, 285
TfiI	GAWTC	8 cut(s)	31, 265, 721, 982, 1141, 1351, 1456, 1531
Tru1I	TTAA	6 cut(s)	45, 248, 468, 1086, 1634, 1680
Tru9I	TTAA	6 cut(s)	45, 248, 468, 1086, 1634, 1680
TscAI	CASTG	3 cut(s)	718, 1500, 1695
TseFI	GTSAC	3 cut(s)	1123, 1228, 1412
TseI	GCWGC	4 cut(s)	522, 627, 1501, 1606
Tsp45I	GTSAC	3 cut(s)	1123, 1228, 1412
TspDTI	ATGAA	8 cut(s)	118, 527, 713, 794, 1004, 1343, 1394, 1448
TspGWI	ACGGA	4 cut(s)	265, 453, 1289, 1539
TspRI	CASTG	3 cut(s)	718, 1500, 1695
Tth111I	GACNNNGTC	1 cut(s)	1624
XapI	RAATTY	2 cut(s)	1165, 1676
XhoI	CTCGAG	1 cut(s)	1668
XmiI	GTMKAC	1 cut(s)	704
XmnI	GAANNNNTTC	3 cut(s)	395, 872, 1397
XspI	CTAG	1 cut(s)	1155

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.