Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000001

Orthogroup ID

OG0013053

Physical Location & Seq

Genomic Coordinates Forward (+)

20409161 .. 20410867

Gene Structure 1707 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_1G00016400.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1707 bp

ATGTCACCAACGAACGAAGCTCTTGTCCTCATTCAAAAAATGGTGAAAAGCCCGCCACTGAAAGCCTTGTCAATCTACAACTCCTCCACTCTCCAAGGCTTCAACCACACCCACCACTCCATCTCCTTCATTCTTCACAACCTCATCTCCTCCAATATGCCAATGCACGCCCAGTCCCTCATTCTTCAACTCCTCTCCGGTCGAATCTCATCCCCATTCTTCACTCCCTCGTCTCTCCTCCACGATTTAACTCAACCCAGGTTGAGCTCAATCCCCACCTCTGGTCATCTATACGAAGCAATCATCAACGCACATGTTCAATCTCAGTTGCCAGAGCAAGCCCTTTACTATTTGAACAAAATGGTTGAGCAAGGCCTCGTTCCCAGATCAAACACTTTCAATAAAGTATTGGGTTTCCTTGTTAAGTCGAGAGATTTTGAGAAGGCTTGGTGGGTGTTCAATGAGTTTAAAGGTAAGGTTGGATTGGACTTGTATAGTTTTGGGATAGTTATAAAATGTTGCTGCGAGGGTGGTGAATTGGATAGGGGTTTTAGGGTTTTGGATGAGATGGAGGAAATGGGTTGGTCTCCGAATGTGGTTATATACACTACATTGATTGATGGGTGTTGCAAGAATGGTGATTTAGAGAGGGCAGAGAAGATGTTTTGTAAGATGGGGGAGCTTGGTTTGGTTGCTAATCAGTTTACATATACTGTGTTGATTGATGGGCTTTTCAAGAAAGGTCGGAATAAAGAAGGGTTTGAGTTGTATGAGAAGATGAAGATTAATGGGGTTGTTCCTAACATGCGTACTTATACTTGTTTGATTAGTGGGTGTTGCAACGATGGAAAAATGAATAGTGCCTTGAACCTGTTCGATGAAATGCGTGAGGGAGGAATGGCCTGCAATGTGGTCACATACAACATCCTAATTGGTGGGTTGTGTCGAGAAATGAGGGTTTGGGAAGCAGAGAAGTTTGTGAATGAGATGAAGAGGGATGGTATTAGTCCTAGTATAGTTACTTTCAACACTCTGATTGATGGGTTTTGTGGTCTTGGAAAGTTGGACAAGGCGTTGAGTTTGTTTGATCAGCTGAAGTCGAGTGGACAATCTCTGTCTCTGGTGACTTATAATGTTCTGATTCAAGGCTTTGCCAAAGCACGAAACTCTGCTGGAGTTATTGATTTGGTGAGAGAGATGGAGAACAGAGGCATGTCTCCTTCTAAAGTGACGTATACAATTGTAATTGATGCCTTGGTTCGATCTGATGACATGGAAAGAGCATTTCATGTGTTTTCCTCCATGGGGAAGGCTGGTCTGGTGCCTGATGTCTACACCTATGGGGTCTTGATACATGGATTATGCATGAAAGGTAATATGATTGAAGCATCAAAGCTGTTACAATCAATGAGCGATTCACATTTAGAGCCAAGTGATGTCATATTTAATATAATGATTCATGGCTACTGCAAAGAGGGTAGCTCTTACAGGGCACTGAGGTTGCTCAAAGAAATGAGAAAGAAAGGAATGGTTCCGAATGTGGCTAGTTACAGTTCGACCATTGGAGTTCTTTGTAATGATGGGAAATGGGAAGAGGCTGAAATGCTACTTCAAGAAATGATGGAGTCAGATTTGAAACCATCAGTTTCTTTGTATAATATAATTTCTAGAGTGAAAAATGATACACTGGTGAAGTTATCAACCTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000959 GO:0001101 GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0004518 GO:0004519 GO:0005488 GO:0006139 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009451 GO:0009719 GO:0009725 GO:0009737 GO:0009987 GO:0010033 GO:0016070 GO:0016071 GO:0016556 GO:0016787 GO:0016788 GO:0033993 GO:0034641 GO:0042221 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0046483 GO:0050896 GO:0071704 GO:0080156 GO:0090304 GO:0090305 GO:0097159 GO:0097305 GO:1900864 GO:1901360 GO:1901363 GO:1901700

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PPR PPR_1 PPR_2 PPR_3 PPR_long

Protein Analysis

568

Amino Acids

63.45

Weight (kDa)

7.14

Isoelectric Point (pI)

40.41

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PPR_long	PF17177	159 - 283	1.2e-08	Pentacotripeptide-repeat region of PRORP
PPR_3	PF13812	185 - 242	1.4e-08	Pentatricopeptide repeat domain
PPR_1	PF12854	194 - 225	1e-12	PPR repeat
PPR_2	PF13041	197 - 246	3.9e-17	PPR repeat family
PPR	PF01535	200 - 230	1.2e-09	PPR repeat
PPR_long	PF17177	205 - 319	5.9e-08	Pentacotripeptide-repeat region of PRORP
PPR_1	PF12854	229 - 261	1.1e-07	PPR repeat
PPR_2	PF13041	233 - 281	1.4e-16	PPR repeat family
PPR	PF01535	235 - 265	2.9e-06	PPR repeat
PPR_3	PF13812	256 - 312	1.2e-11	Pentatricopeptide repeat domain
PPR_1	PF12854	263 - 296	2.4e-12	PPR repeat
PPR_2	PF13041	271 - 316	1.4e-17	PPR repeat family
PPR	PF01535	271 - 299	2.7e-08	PPR repeat
TPR_24	PF23276	272 - 364	5.1e-06	Fungal tetratrico peptide repeats
PPR_1	PF12854	299 - 331	2.5e-12	PPR repeat
PPR_2	PF13041	303 - 350	1.2e-17	PPR repeat family
PPR	PF01535	305 - 335	6e-08	PPR repeat
PPR_3	PF13812	327 - 384	7.4e-10	Pentatricopeptide repeat domain
PPR_1	PF12854	333 - 365	2.1e-11	PPR repeat
PPR_2	PF13041	337 - 386	1.2e-15	PPR repeat family
PPR	PF01535	340 - 369	5.1e-08	PPR repeat
PPR_long	PF17177	355 - 479	5.4e-11	Pentacotripeptide-repeat region of PRORP
PPR_2	PF13041	374 - 421	5.6e-13	PPR repeat family
PPR_3	PF13812	396 - 452	9.3e-09	Pentatricopeptide repeat domain
PPR_2	PF13041	410 - 455	8.6e-15	PPR repeat family
PPR_3	PF13812	437 - 489	5.2e-06	Pentatricopeptide repeat domain
PPR_1	PF12854	439 - 470	1.2e-09	PPR repeat
PPR_3	PF13812	465 - 524	5e-08	Pentatricopeptide repeat domain
PPR_1	PF12854	475 - 506	1.9e-08	PPR repeat
PPR_2	PF13041	477 - 526	4.2e-14	PPR repeat family
PPR	PF01535	480 - 509	3.4e-08	PPR repeat
PPR_3	PF13812	500 - 555	1.9e-06	Pentatricopeptide repeat domain
PPR_1	PF12854	508 - 540	3.5e-06	PPR repeat

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0013053)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G11690 AT4G11690
fragaria_vesca	FvH4_3g36170
malus_domestica	MD03G1092900.v1.1
prunus_persica	Prupe.6G076800_v2.0.a1
pyrus_communis	pycom03g07420
rosa_chinensis	RchiOBHm_Chr5g0064671 RchiOBHm_Chr5g0064911
rosa_laevigata	RLG00000035740 RLG00000035742
rosa_multiflora	Rmu_sc0020903.1_g000003 Rmu_sc0025431.1_g000001
rosa_roxburghii	Rroxscaffold_1G00016400
rosa_rugosa	Rorug05G0366600
rosa_samantha	Rh5AG425100 Rh5CG462600 Rh5DG453100
rosa_wichuraiana	Rw5G039940

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	512, 1131
AccB1I	GGYRCC	1 cut(s)	1321
AccI	GTMKAC	2 cut(s)	1235, 1332
AciI	CCGC	1 cut(s)	53
AcuI	CTGAAG	1 cut(s)	1115
AfaI	GTAC	1 cut(s)	811
AfiI	CCNNNNNNNGG	2 cut(s)	281, 1305
AflIII	ACRYGT	1 cut(s)	313
AjnI	CCWGG	1 cut(s)	257
AluBI	AGCT	6 cut(s)	20, 267, 682, 1093, 1396, 1482
AluI	AGCT	6 cut(s)	20, 267, 682, 1093, 1396, 1482
Alw21I	GWGCWC	1 cut(s)	269
Alw26I	GTCTC	4 cut(s)	237, 591, 1122, 1221
AoxI	GGCC	2 cut(s)	373, 900
ApeKI	GCWGC	1 cut(s)	522
ArsI	GACNNNNNNTTYG	2 cut(s)	9, 41
AseI	ATTAAT	1 cut(s)	786
Asp700I	GAANNNNTTC	1 cut(s)	872
AsuHPI	GGTGA	6 cut(s)	55, 545, 650, 1135, 1201, 1702
BaeGI	GKGCMC	1 cut(s)	1495
BaeI	ACNNNNGTAYC	2 cut(s)	1674, 1707
BanI	GGYRCC	1 cut(s)	1321
BanII	GRGCYC	1 cut(s)	269
Bbv12I	GWGCWC	1 cut(s)	269
BbvI	GCAGC	1 cut(s)	509
BciT130I	CCWGG	1 cut(s)	259
BclI	TGATCA	1 cut(s)	1087
BcoDI	GTCTC	4 cut(s)	237, 591, 1122, 1221
BfaI	CTAG	3 cut(s)	1011, 1545, 1668
BisI	GCNGC	1 cut(s)	523
BlsI	GCNGC	1 cut(s)	524
Bme1390I	CCNGG	1 cut(s)	259
BmiI	GGNNCC	2 cut(s)	1323, 1533
BmrFI	CCNGG	1 cut(s)	259
BmrI	ACTGGG	1 cut(s)	166
BmsI	GCATC	2 cut(s)	1240, 1397
BmuI	ACTGGG	1 cut(s)	166
BplI	GAGNNNNNCTC	2 cut(s)	1466, 1498
BpmI	CTGGAG	1 cut(s)	1194
BsaI	GGTCTC	1 cut(s)	591
BsaJI	CCNNGG	4 cut(s)	94, 257, 1254, 1302
BsaWI	WCCGGW	1 cut(s)	197
BsaXI	ACNNNNNCTCC	6 cut(s)	68, 98, 107, 131, 137, 161
Bsc4I	CCNNNNNNNGG	2 cut(s)	281, 1305
Bse1I	ACTGG	2 cut(s)	172, 1692
Bse3DI	GCAATG	1 cut(s)	913
BseBI	CCWGG	1 cut(s)	259
BseDI	CCNNGG	4 cut(s)	94, 257, 1254, 1302
BseGI	GGATG	4 cut(s)	209, 568, 924, 1003
BseLI	CCNNNNNNNGG	2 cut(s)	281, 1305
BseMI	GCAATG	1 cut(s)	913
BseMII	CTCAG	2 cut(s)	338, 1487
BseNI	ACTGG	2 cut(s)	172, 1692
BseRI	GAGGAG	4 cut(s)	73, 139, 182, 227
BseSI	GKGCMC	1 cut(s)	1495
BseXI	GCAGC	1 cut(s)	509
Bsh1285I	CGRYCG	1 cut(s)	202
BshFI	GGCC	2 cut(s)	375, 902
BshNI	GGYRCC	1 cut(s)	1321
BsiEI	CGRYCG	1 cut(s)	202
BsiHKAI	GWGCWC	1 cut(s)	269
BsiSI	CCGG	1 cut(s)	198
BslFI	GGGAC	1 cut(s)	160
BslI	CCNNNNNNNGG	2 cut(s)	281, 1305
BsmAI	GTCTC	4 cut(s)	237, 591, 1122, 1221
BsmBI	CGTCTC	1 cut(s)	237
BsmFI	GGGAC	1 cut(s)	160
BsnI	GGCC	2 cut(s)	375, 902
Bso31I	GGTCTC	1 cut(s)	591
Bsp1286I	GDGCHC	2 cut(s)	269, 1495
Bsp143I	GATC	3 cut(s)	386, 1087, 1262
Bsp19I	CCATGG	1 cut(s)	1302
BspACI	CCGC	1 cut(s)	53
BspANI	GGCC	2 cut(s)	375, 902
BspCNI	CTCAG	2 cut(s)	337, 1488
BspLI	GGNNCC	2 cut(s)	1323, 1533
BspT107I	GGYRCC	1 cut(s)	1321
BspTNI	GGTCTC	1 cut(s)	591
BsrDI	GCAATG	1 cut(s)	913
BsrI	ACTGG	2 cut(s)	172, 1692
BssECI	CCNNGG	4 cut(s)	94, 257, 1254, 1302
BssMI	GATC	3 cut(s)	386, 1087, 1262
BssNAI	GTATAC	1 cut(s)	1236
BssT1I	CCWWGG	3 cut(s)	94, 1254, 1302
Bst1107I	GTATAC	1 cut(s)	1236
Bst2UI	CCWGG	1 cut(s)	259
Bst4CI	ACNGT	2 cut(s)	715, 1553
Bst6I	CTCTTC	2 cut(s)	986, 1587
BstC8I	GCNNGC	4 cut(s)	53, 168, 339, 904
BstDEI	CTNAG	2 cut(s)	324, 1496
BstDSI	CCRYGG	1 cut(s)	1302
BstF5I	GGATG	4 cut(s)	209, 568, 924, 1003
BstKTI	GATC	3 cut(s)	389, 1090, 1265
BstMAI	GTCTC	4 cut(s)	237, 591, 1122, 1221
BstMBI	GATC	3 cut(s)	386, 1087, 1262
BstMCI	CGRYCG	1 cut(s)	202
BstNI	CCWGG	1 cut(s)	259
BstNSI	RCATGY	3 cut(s)	317, 808, 1216
BstSCI	CCNGG	1 cut(s)	257
BstSLI	GKGCMC	1 cut(s)	1495
BstV1I	GCAGC	1 cut(s)	509
BstZ17I	GTATAC	1 cut(s)	1236
BsuRI	GGCC	2 cut(s)	375, 902
BtgI	CCRYGG	1 cut(s)	1302
BtsCI	GGATG	4 cut(s)	209, 568, 924, 1003
BtsIMutI	CAGTG	3 cut(s)	56, 1493, 1685
Cac8I	GCNNGC	4 cut(s)	53, 168, 339, 904
Csp6I	GTAC	1 cut(s)	810
CviAII	CATG	9 cut(s)	314, 805, 1213, 1273, 1289, 1303, 1355, 1366, 1460
CviQI	GTAC	1 cut(s)	810
DdeI	CTNAG	2 cut(s)	324, 1496
DpnI	GATC	3 cut(s)	388, 1089, 1264
DpnII	GATC	3 cut(s)	386, 1087, 1262
DraI	TTTAAA	1 cut(s)	469
Eam1104I	CTCTTC	2 cut(s)	986, 1587
EarI	CTCTTC	2 cut(s)	986, 1587
Ecl136II	GAGCTC	1 cut(s)	267
Eco130I	CCWWGG	3 cut(s)	94, 1254, 1302
Eco147I	AGGCCT	1 cut(s)	375
Eco24I	GRGCYC	1 cut(s)	269
Eco31I	GGTCTC	1 cut(s)	591
Eco53kI	GAGCTC	1 cut(s)	267
Eco57I	CTGAAG	1 cut(s)	1115
EcoICRI	GAGCTC	1 cut(s)	267
EcoRII	CCWGG	1 cut(s)	257
EcoT14I	CCWWGG	3 cut(s)	94, 1254, 1302
EcoT22I	ATGCAT	1 cut(s)	1367
EcoT38I	GRGCYC	1 cut(s)	269
ErhI	CCWWGG	3 cut(s)	94, 1254, 1302
Esp3I	CGTCTC	1 cut(s)	237
FaeI	CATG	9 cut(s)	317, 808, 1216, 1276, 1292, 1306, 1358, 1369, 1463
FaqI	GGGAC	1 cut(s)	160
FatI	CATG	9 cut(s)	313, 804, 1212, 1272, 1288, 1302, 1354, 1365, 1459
FauI	CCCGC	1 cut(s)	60
FbaI	TGATCA	1 cut(s)	1087
FblI	GTMKAC	2 cut(s)	1235, 1332
Fnu4HI	GCNGC	1 cut(s)	523
FokI	GGATG	4 cut(s)	196, 575, 911, 1010
FriOI	GRGCYC	1 cut(s)	269
Fsp4HI	GCNGC	1 cut(s)	523
FspBI	CTAG	3 cut(s)	1011, 1545, 1668
GluI	GCNGC	1 cut(s)	523
GsuI	CTGGAG	1 cut(s)	1194
HaeIII	GGCC	2 cut(s)	375, 902
HapII	CCGG	1 cut(s)	198
Hin1II	CATG	9 cut(s)	317, 808, 1216, 1276, 1292, 1306, 1358, 1369, 1463
HinfI	GANTC	5 cut(s)	204, 1141, 1415, 1456, 1625
HpaII	CCGG	1 cut(s)	198
HphI	GGTGA	6 cut(s)	55, 545, 650, 1135, 1201, 1702
Hpy166II	GTNNAC	4 cut(s)	705, 1106, 1236, 1333
Hpy188I	TCNGA	7 cut(s)	591, 747, 1035, 1140, 1267, 1536, 1630
Hpy188III	TCNNGA	6 cut(s)	429, 736, 947, 1348, 1613, 1668
Hpy8I	GTNNAC	4 cut(s)	705, 1106, 1236, 1333
HpyAV	CCTTC	5 cut(s)	136, 436, 749, 1230, 1303
HpyCH4III	ACNGT	2 cut(s)	715, 1553
HpyCH4IV	ACGT	1 cut(s)	1232
HpyCH4V	TGCA	6 cut(s)	166, 630, 840, 906, 1365, 1470
HpyF3I	CTNAG	2 cut(s)	324, 1496
HpySE526I	ACGT	1 cut(s)	1232
Hsp92II	CATG	9 cut(s)	317, 808, 1216, 1276, 1292, 1306, 1358, 1369, 1463
Ksp22I	TGATCA	1 cut(s)	1087
Kzo9I	GATC	3 cut(s)	386, 1087, 1262
LmnI	GCTCC	1 cut(s)	679
Lsp1109I	GCAGC	1 cut(s)	509
LweI	GCATC	2 cut(s)	1240, 1397
MaeI	CTAG	3 cut(s)	1011, 1545, 1668
MaeII	ACGT	1 cut(s)	1232
MaeIII	GTNAC	7 cut(s)	3, 913, 1018, 1123, 1228, 1398, 1547
MalI	GATC	3 cut(s)	388, 1089, 1264
MboI	GATC	3 cut(s)	386, 1087, 1262
MboII	GAAGA	8 cut(s)	125, 176, 211, 670, 787, 793, 1003, 1604
MfeI	CAATTG	1 cut(s)	1239
MhlI	GDGCHC	2 cut(s)	269, 1495
MluCI	AATT	5 cut(s)	536, 930, 1239, 1245, 1662
MlyI	GAGTC	1 cut(s)	1634
MmeI	TCCRAC	3 cut(s)	460, 725, 1044
Mph1103I	ATGCAT	1 cut(s)	1367
MroXI	GAANNNNTTC	1 cut(s)	872
MseI	TTAA	5 cut(s)	248, 423, 468, 786, 1446
MspA1I	CMGCKG	1 cut(s)	1093
MspI	CCGG	1 cut(s)	198
MspR9I	CCNGG	1 cut(s)	259
MunI	CAATTG	1 cut(s)	1239
MvaI	CCWGG	1 cut(s)	259
NcoI	CCATGG	1 cut(s)	1302
NdeII	GATC	3 cut(s)	386, 1087, 1262
NlaIII	CATG	9 cut(s)	317, 808, 1216, 1276, 1292, 1306, 1358, 1369, 1463
NlaIV	GGNNCC	2 cut(s)	1323, 1533
NmuCI	GTSAC	4 cut(s)	3, 913, 1123, 1228
NsiI	ATGCAT	1 cut(s)	1367
NspI	RCATGY	3 cut(s)	317, 808, 1216
PceI	AGGCCT	1 cut(s)	375
PciI	ACATGT	1 cut(s)	313
PdmI	GAANNNNTTC	1 cut(s)	872
PfeI	GAWTC	4 cut(s)	204, 1141, 1415, 1456
PkrI	GCNGC	1 cut(s)	524
PleI	GAGTC	1 cut(s)	1633
PpsI	GAGTC	1 cut(s)	1633
PscI	ACATGT	1 cut(s)	313
PshBI	ATTAAT	1 cut(s)	786
PsiI	TTATAA	2 cut(s)	512, 1131
Psp124BI	GAGCTC	1 cut(s)	269
Psp6I	CCWGG	1 cut(s)	257
PspGI	CCWGG	1 cut(s)	257
PspN4I	GGNNCC	2 cut(s)	1323, 1533
PvuII	CAGCTG	1 cut(s)	1093
RsaI	GTAC	1 cut(s)	811
RsaNI	GTAC	1 cut(s)	810
SacI	GAGCTC	1 cut(s)	269
SaqAI	TTAA	5 cut(s)	248, 423, 468, 786, 1446
SatI	GCNGC	1 cut(s)	523
Sau3AI	GATC	3 cut(s)	386, 1087, 1262
SchI	GAGTC	1 cut(s)	1634
ScrFI	CCNGG	1 cut(s)	259
SduI	GDGCHC	2 cut(s)	269, 1495
SfaNI	GCATC	2 cut(s)	1240, 1397
Sse9I	AATT	5 cut(s)	536, 930, 1239, 1245, 1662
SseBI	AGGCCT	1 cut(s)	375
SsiI	CCGC	1 cut(s)	53
SspMI	CTAG	3 cut(s)	1011, 1545, 1668
SstI	GAGCTC	1 cut(s)	269
StuI	AGGCCT	1 cut(s)	375
StyD4I	CCNGG	1 cut(s)	257
StyI	CCWWGG	3 cut(s)	94, 1254, 1302
TaaI	ACNGT	2 cut(s)	715, 1553
TaiI	ACGT	1 cut(s)	1235
TaqI	TCGA	7 cut(s)	202, 428, 876, 946, 1100, 1261, 1556
TasI	AATT	5 cut(s)	536, 930, 1239, 1245, 1662
TfiI	GAWTC	4 cut(s)	204, 1141, 1415, 1456
Tru1I	TTAA	5 cut(s)	248, 423, 468, 786, 1446
Tru9I	TTAA	5 cut(s)	248, 423, 468, 786, 1446
TscAI	CASTG	3 cut(s)	63, 1500, 1692
TseFI	GTSAC	4 cut(s)	3, 913, 1123, 1228
TseI	GCWGC	1 cut(s)	522
Tsp45I	GTSAC	4 cut(s)	3, 913, 1123, 1228
TspDTI	ATGAA	8 cut(s)	118, 794, 869, 894, 1004, 1277, 1382, 1448
TspRI	CASTG	3 cut(s)	63, 1500, 1692
VspI	ATTAAT	1 cut(s)	786
XbaI	TCTAGA	1 cut(s)	1667
XceI	RCATGY	3 cut(s)	317, 808, 1216
XmiI	GTMKAC	2 cut(s)	1235, 1332
XmnI	GAANNNNTTC	1 cut(s)	872
XspI	CTAG	3 cut(s)	1011, 1545, 1668
Zsp2I	ATGCAT	1 cut(s)	1367

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.