RLG00000035740

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0013053
Physical Location & Seq
Reverse (-)
71098730 .. 71100199
1470 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000035740

Sequence Viewer

Length: 1470 bp
ATGGTGAAAAGCCCGCCACTGAAAGCCTTGTCAATCTTCAACTCCTCAACTCTCCAAGGCTTCCACCACACCCACCACTCCATCTCCTTCATTCTTCACAACCTCATCTCCTCCAATATGCCAATGCACGCCCAGTCCCTCATTCTTCAACTCCTCTCCGGTCGAATCTCATCCCCATTCTTCACTCCCTCGTCTCTCCTCCACGATTTAACTCAATCCAAGTTGAGCTCAGTCCCCACCTCTGGTCATCTATACGAAGCAATCATCAACGCCCATGTTCAATCTCAGTTGCCAGAGCAAGCCCTTTACTATTTGAACAAAATGGTTGAGCAAGGCCTCGTTCCCAGATCAAACACTTTCAATAACGTATTGGGTTTTCTTGTAAAGTCGAGAGATTTTGAAAAGGCTTGGTGGGTGTTCAATGAGTTTAAAGGCAAGGTGGGATTGGATTTGTATAGTTTTGGGATAGTTATAAAAAGTTGCTGCGAGGGTAGTGAATTGGATAGGGGTTTTGGGGTTTTGGATGAGATGGAGGAAATGGGTTGGTCTCCTAATGTGGTTATATATACTACATTGATTGATGGGTGTTGCAAGAATGGTGATTTAGAGAGGGCAATTAAGATGTTTTGTAAAATGGGGGAGCTTGGTTTGGTTGCTAATCAGTTTACATATACTGTGTTGATTGATGGGCTTTTCAAGAAAGGTCGGAATAAAGAAGGGTTTGAGTTGTATGAGAAGATGAAGATTAATGGGGTTGTTCCTAACATGCGTACTTATACTTGTTTGATCAGTGGGTGTTGCAACGATGGAAAAATGAATACTGCCTTGAAACTGTTCGATGAAATGCGTGAGAGAGGAATGGCCTGCAATGTGGTCACATACAACATCCTAATTGGTGGGTTGTGTCGAGAAATGAGGGTTTGGGAAGCTGAGAAGTTTGTGAATGAGATGAAGAGGGATGGTATTAGTCCAAGTATAGTTACTTTCAACACTCTGATTGATGGGTTTTGTGGTCATGGAAAGTTGGACAAGGCGTTGAGTTTGTTTGATCAGTTGAAGTCGAGTGGAGAGTCTCCGTCTCTGGTGACTTATAATGTTCTGATTCAAGGATTTGCCAAAGCTCGAAACTCTGCTGGAGTTACTGATTTGGTTAGAGAGATGGAGAACAGAGGCATGTCTCCTTCTAAAGTGACATATACAATTGTAATTGATGCGTTGGTTCGATCTGATGACATGGAAAGAGCATTTCATGTGTTCTCCTCCATGAAGAAGGCTGGTCTGGTGCCTGATGTCTACACCTATGGGGTGTTAATACATGGATTATGCATGAAAGGTAATATGATTGAAGCATCAAAGCTGTTACAATCAATGAGCGATTCACATTTGGAGCCAAGTGATGTCATATTTAATATAATGATTCATGGCTACTGCAAGGAGGGTAGCTCTTACAGGGCACTGAGGTTGCTTTAA

Protein Analysis

490

Amino Acids

54.64

Weight (kDa)

7.55

Isoelectric Point (pI)

41.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_long PF17177 147 - 271 8.8e-08 Pentacotripeptide-repeat region of PRORP
PPR_3 PF13812 173 - 229 5.3e-08 Pentatricopeptide repeat domain
PPR_1 PF12854 181 - 212 7.9e-13 PPR repeat
PPR_2 PF13041 184 - 233 1.4e-17 PPR repeat family
PPR PF01535 187 - 217 1.3e-09 PPR repeat
PPR_long PF17177 192 - 305 5.6e-10 Pentacotripeptide-repeat region of PRORP
PPR_1 PF12854 216 - 248 9e-08 PPR repeat
PPR_2 PF13041 220 - 268 1.2e-16 PPR repeat family
PPR PF01535 222 - 252 2.4e-06 PPR repeat
PPR_3 PF13812 243 - 299 1.8e-12 Pentatricopeptide repeat domain
PPR_1 PF12854 250 - 283 6.9e-13 PPR repeat
PPR_2 PF13041 258 - 303 9.5e-19 PPR repeat family
PPR PF01535 258 - 286 1.5e-09 PPR repeat
TPR_24 PF23276 259 - 351 5.3e-06 Fungal tetratrico peptide repeats
PPR_1 PF12854 285 - 318 1e-12 PPR repeat
PPR_2 PF13041 290 - 337 1e-17 PPR repeat family
PPR PF01535 292 - 322 4.9e-08 PPR repeat
PPR_3 PF13812 314 - 371 1e-12 Pentatricopeptide repeat domain
PPR_1 PF12854 320 - 352 2.8e-12 PPR repeat
PPR_2 PF13041 324 - 373 1.9e-17 PPR repeat family
PPR PF01535 327 - 356 1.3e-08 PPR repeat
PPR_long PF17177 345 - 482 6.8e-10 Pentacotripeptide-repeat region of PRORP
PPR_2 PF13041 359 - 408 5.7e-13 PPR repeat family
TPR_24 PF23276 380 - 488 9.9e-09 Fungal tetratrico peptide repeats
PPR_3 PF13812 383 - 438 3.2e-10 Pentatricopeptide repeat domain
PPR PF01535 397 - 427 3.5e-06 PPR repeat
PPR_2 PF13041 397 - 442 3.6e-16 PPR repeat family
PPR_3 PF13812 422 - 476 1.4e-06 Pentatricopeptide repeat domain
PPR_1 PF12854 426 - 457 1e-09 PPR repeat
PPR_2 PF13041 437 - 478 2.3e-08 PPR repeat family
PPR_1 PF12854 462 - 489 9.4e-06 PPR repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0013053)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G11690 AT4G11690
fragaria_vesca FvH4_3g36170
malus_domestica MD03G1092900.v1.1
prunus_persica Prupe.6G076800_v2.0.a1
pyrus_communis pycom03g07420
rosa_chinensis RchiOBHm_Chr5g0064671 RchiOBHm_Chr5g0064911
rosa_laevigata RLG00000035740 RLG00000035742
rosa_multiflora Rmu_sc0020903.1_g000003 Rmu_sc0025431.1_g000001
rosa_roxburghii Rroxscaffold_1G00016400
rosa_rugosa Rorug05G0366600
rosa_samantha Rh5AG425100 Rh5CG462600 Rh5DG453100
rosa_wichuraiana Rw5G039940

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 473, 1092
AccB1I GGYRCC 1 cut(s) 1282
AccI GTMKAC 1 cut(s) 1293
AciI CCGC 1 cut(s) 14
AfaI GTAC 1 cut(s) 772
AfiI CCNNNNNNNGG 1 cut(s) 242
AluBI AGCT 6 cut(s) 228, 643, 929, 1121, 1357, 1443
AluI AGCT 6 cut(s) 228, 643, 929, 1121, 1357, 1443
Alw21I GWGCWC 1 cut(s) 230
Alw26I GTCTC 5 cut(s) 198, 552, 1077, 1083, 1182
AoxI GGCC 2 cut(s) 334, 861
ApeKI GCWGC 1 cut(s) 483
AseI ATTAAT 1 cut(s) 747
Asp700I GAANNNNTTC 1 cut(s) 833
AsuHPI GGTGA 3 cut(s) 16, 611, 1096
BaeGI GKGCMC 1 cut(s) 1456
BanI GGYRCC 1 cut(s) 1282
BanII GRGCYC 1 cut(s) 230
Bbv12I GWGCWC 1 cut(s) 230
BbvI GCAGC 1 cut(s) 470
BccI CCATC 8 cut(s) 89, 523, 575, 680, 800, 953, 995, 1153
BclI TGATCA 2 cut(s) 786, 1048
BcoDI GTCTC 5 cut(s) 198, 552, 1077, 1083, 1182
BisI GCNGC 1 cut(s) 484
BlsI GCNGC 1 cut(s) 485
BmiI GGNNCC 2 cut(s) 1284, 1389
BmrI ACTGGG 1 cut(s) 127
BmsI GCATC 2 cut(s) 1201, 1358
BmuI ACTGGG 1 cut(s) 127
BplI GAGNNNNNCTC 2 cut(s) 1427, 1459
BpmI CTGGAG 1 cut(s) 1155
BsaI GGTCTC 1 cut(s) 552
BsaJI CCNNGG 1 cut(s) 55
BsaWI WCCGGW 1 cut(s) 158
BsaXI ACNNNNNCTCC 4 cut(s) 68, 92, 98, 122
Bsc4I CCNNNNNNNGG 1 cut(s) 242
Bse1I ACTGG 1 cut(s) 133
Bse3DI GCAATG 1 cut(s) 874
BseDI CCNNGG 1 cut(s) 55
BseGI GGATG 4 cut(s) 170, 529, 885, 964
BseLI CCNNNNNNNGG 1 cut(s) 242
BseMI GCAATG 1 cut(s) 874
BseMII CTCAG 4 cut(s) 243, 299, 921, 1448
BseNI ACTGG 1 cut(s) 133
BseRI GAGGAG 5 cut(s) 34, 100, 143, 188, 1249
BseSI GKGCMC 1 cut(s) 1456
BseXI GCAGC 1 cut(s) 470
Bsh1285I CGRYCG 1 cut(s) 163
BshFI GGCC 2 cut(s) 336, 863
BshNI GGYRCC 1 cut(s) 1282
BsiEI CGRYCG 1 cut(s) 163
BsiHKAI GWGCWC 1 cut(s) 230
BsiSI CCGG 1 cut(s) 159
BslFI GGGAC 2 cut(s) 121, 218
BslI CCNNNNNNNGG 1 cut(s) 242
BsmAI GTCTC 5 cut(s) 198, 552, 1077, 1083, 1182
BsmBI CGTCTC 2 cut(s) 198, 1083
BsmFI GGGAC 2 cut(s) 121, 218
BsnI GGCC 2 cut(s) 336, 863
Bso31I GGTCTC 1 cut(s) 552
Bsp1286I GDGCHC 2 cut(s) 230, 1456
Bsp143I GATC 4 cut(s) 347, 786, 1048, 1223
BspACI CCGC 1 cut(s) 14
BspANI GGCC 2 cut(s) 336, 863
BspCNI CTCAG 4 cut(s) 242, 298, 922, 1449
BspLI GGNNCC 2 cut(s) 1284, 1389
BspT107I GGYRCC 1 cut(s) 1282
BspTNI GGTCTC 1 cut(s) 552
BsrDI GCAATG 1 cut(s) 874
BsrI ACTGG 1 cut(s) 133
BssECI CCNNGG 1 cut(s) 55
BssMI GATC 4 cut(s) 347, 786, 1048, 1223
BssT1I CCWWGG 1 cut(s) 55
Bst4CI ACNGT 2 cut(s) 676, 834
Bst6I CTCTTC 1 cut(s) 947
BstC8I GCNNGC 4 cut(s) 14, 129, 300, 865
BstDEI CTNAG 4 cut(s) 229, 285, 930, 1457
BstF5I GGATG 4 cut(s) 170, 529, 885, 964
BstKTI GATC 4 cut(s) 350, 789, 1051, 1226
BstMAI GTCTC 5 cut(s) 198, 552, 1077, 1083, 1182
BstMBI GATC 4 cut(s) 347, 786, 1048, 1223
BstMCI CGRYCG 1 cut(s) 163
BstNSI RCATGY 2 cut(s) 769, 1177
BstSLI GKGCMC 1 cut(s) 1456
BstV1I GCAGC 1 cut(s) 470
BsuRI GGCC 2 cut(s) 336, 863
BtsCI GGATG 4 cut(s) 170, 529, 885, 964
BtsIMutI CAGTG 3 cut(s) 17, 796, 1454
Cac8I GCNNGC 4 cut(s) 14, 129, 300, 865
Csp6I GTAC 1 cut(s) 771
CviQI GTAC 1 cut(s) 771
DdeI CTNAG 4 cut(s) 229, 285, 930, 1457
DpnI GATC 4 cut(s) 349, 788, 1050, 1225
DpnII GATC 4 cut(s) 347, 786, 1048, 1223
DraI TTTAAA 1 cut(s) 430
Eam1104I CTCTTC 1 cut(s) 947
EarI CTCTTC 1 cut(s) 947
Ecl136II GAGCTC 1 cut(s) 228
Eco130I CCWWGG 1 cut(s) 55
Eco147I AGGCCT 1 cut(s) 336
Eco24I GRGCYC 1 cut(s) 230
Eco31I GGTCTC 1 cut(s) 552
Eco53kI GAGCTC 1 cut(s) 228
EcoICRI GAGCTC 1 cut(s) 228
EcoT14I CCWWGG 1 cut(s) 55
EcoT22I ATGCAT 1 cut(s) 1328
EcoT38I GRGCYC 1 cut(s) 230
ErhI CCWWGG 1 cut(s) 55
Esp3I CGTCTC 2 cut(s) 198, 1083
FaqI GGGAC 2 cut(s) 121, 218
FauI CCCGC 1 cut(s) 21
FbaI TGATCA 2 cut(s) 786, 1048
FblI GTMKAC 1 cut(s) 1293
Fnu4HI GCNGC 1 cut(s) 484
FokI GGATG 4 cut(s) 157, 536, 872, 971
FriOI GRGCYC 1 cut(s) 230
Fsp4HI GCNGC 1 cut(s) 484
GluI GCNGC 1 cut(s) 484
GsuI CTGGAG 1 cut(s) 1155
HaeIII GGCC 2 cut(s) 336, 863
HapII CCGG 1 cut(s) 159
HinfI GANTC 5 cut(s) 165, 1070, 1102, 1376, 1417
HpaII CCGG 1 cut(s) 159
HphI GGTGA 3 cut(s) 16, 611, 1096
Hpy166II GTNNAC 2 cut(s) 666, 1294
Hpy188I TCNGA 4 cut(s) 708, 996, 1101, 1228
Hpy188III TCNNGA 3 cut(s) 390, 697, 908
Hpy8I GTNNAC 2 cut(s) 666, 1294
HpyAV CCTTC 4 cut(s) 97, 710, 1191, 1264
HpyCH4III ACNGT 2 cut(s) 676, 834
HpyCH4IV ACGT 1 cut(s) 366
HpyCH4V TGCA 6 cut(s) 127, 591, 801, 867, 1326, 1431
HpyF3I CTNAG 4 cut(s) 229, 285, 930, 1457
HpySE526I ACGT 1 cut(s) 366
Ksp22I TGATCA 2 cut(s) 786, 1048
Kzo9I GATC 4 cut(s) 347, 786, 1048, 1223
LmnI GCTCC 2 cut(s) 640, 1387
Lsp1109I GCAGC 1 cut(s) 470
LweI GCATC 2 cut(s) 1201, 1358
MaeII ACGT 1 cut(s) 366
MaeIII GTNAC 6 cut(s) 874, 979, 1084, 1138, 1189, 1359
MalI GATC 4 cut(s) 349, 788, 1050, 1225
MboI GATC 4 cut(s) 347, 786, 1048, 1223
MboII GAAGA 8 cut(s) 28, 86, 137, 172, 748, 754, 964, 1279
MfeI CAATTG 1 cut(s) 1200
MhlI GDGCHC 2 cut(s) 230, 1456
MluCI AATT 5 cut(s) 497, 615, 891, 1200, 1206
MlyI GAGTC 1 cut(s) 1079
MmeI TCCRAC 2 cut(s) 686, 1005
Mph1103I ATGCAT 1 cut(s) 1328
MroXI GAANNNNTTC 1 cut(s) 833
MseI TTAA 7 cut(s) 209, 429, 618, 747, 1310, 1407, 1468
MspI CCGG 1 cut(s) 159
MunI CAATTG 1 cut(s) 1200
NdeII GATC 4 cut(s) 347, 786, 1048, 1223
NlaIV GGNNCC 2 cut(s) 1284, 1389
NmuCI GTSAC 3 cut(s) 874, 1084, 1189
NsiI ATGCAT 1 cut(s) 1328
NspI RCATGY 2 cut(s) 769, 1177
PceI AGGCCT 1 cut(s) 336
PdmI GAANNNNTTC 1 cut(s) 833
PfeI GAWTC 4 cut(s) 165, 1102, 1376, 1417
PkrI GCNGC 1 cut(s) 485
PleI GAGTC 1 cut(s) 1078
PpsI GAGTC 1 cut(s) 1078
PshBI ATTAAT 1 cut(s) 747
PsiI TTATAA 2 cut(s) 473, 1092
Psp124BI GAGCTC 1 cut(s) 230
PspN4I GGNNCC 2 cut(s) 1284, 1389
RsaI GTAC 1 cut(s) 772
RsaNI GTAC 1 cut(s) 771
SacI GAGCTC 1 cut(s) 230
SaqAI TTAA 7 cut(s) 209, 429, 618, 747, 1310, 1407, 1468
SatI GCNGC 1 cut(s) 484
Sau3AI GATC 4 cut(s) 347, 786, 1048, 1223
SchI GAGTC 1 cut(s) 1079
SduI GDGCHC 2 cut(s) 230, 1456
SfaNI GCATC 2 cut(s) 1201, 1358
Sse9I AATT 5 cut(s) 497, 615, 891, 1200, 1206
SseBI AGGCCT 1 cut(s) 336
SsiI CCGC 1 cut(s) 14
SstI GAGCTC 1 cut(s) 230
StuI AGGCCT 1 cut(s) 336
StyI CCWWGG 1 cut(s) 55
TaaI ACNGT 2 cut(s) 676, 834
TaiI ACGT 1 cut(s) 369
TaqI TCGA 7 cut(s) 163, 389, 837, 907, 1061, 1123, 1222
TasI AATT 5 cut(s) 497, 615, 891, 1200, 1206
TfiI GAWTC 4 cut(s) 165, 1102, 1376, 1417
Tru1I TTAA 7 cut(s) 209, 429, 618, 747, 1310, 1407, 1468
Tru9I TTAA 7 cut(s) 209, 429, 618, 747, 1310, 1407, 1468
TscAI CASTG 3 cut(s) 24, 796, 1461
TseFI GTSAC 3 cut(s) 874, 1084, 1189
TseI GCWGC 1 cut(s) 483
Tsp45I GTSAC 3 cut(s) 874, 1084, 1189
TspDTI ATGAA 9 cut(s) 79, 755, 830, 855, 965, 1238, 1280, 1343, 1409
TspGWI ACGGA 1 cut(s) 1065
TspRI CASTG 3 cut(s) 24, 796, 1461
VspI ATTAAT 1 cut(s) 747
XceI RCATGY 2 cut(s) 769, 1177
XmiI GTMKAC 1 cut(s) 1293
XmnI GAANNNNTTC 1 cut(s) 833
Zsp2I ATGCAT 1 cut(s) 1328
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.