MD03G1095400.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0014466
Physical Location & Seq
Forward (+)
8096029 .. 8096568
540 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1095400.v1.1.491

Sequence Viewer

Length: 540 bp
ATGTTGGTTTCTGAGAAAAATGGTACTAATCACGGTCTTAGTCCCATGGAATTCAATTTCTTAGTGAGACCCGCATTGAATTTCAATAATCATGCATGCCAAATTGCTCATTGTGAAGACGAAGAAGAAGATGGAGACGGCCAATTACATAGCTCAGAGGAGAAGCAAGAAGATAATTTCGACATGTTTGTGTCATCGTTGAAGGTCAAAGTTCCATCAGCAGGAGAATTCTGTAGAGTCCAGCAGGAAGGTATTGGTGGTGATGGGGACGTGGAATATGGGTTGAAGACTCCAACATCTTTGGATCACAAAATCAAAGGGAGCCTGCAATGTCCACCAGCACCAAGAAAACCCAAATCACTTCCACTAAAGAAGAGAAAAGCAATACTTGACCCCAGAATTTTACTTGATTTTTCGAGTGATGAGATCGAATCGTTGTTTCCTCCGGTTGATGGTGTTCTTGCAGATCTTGGTAGTAAGATTATCATGAAGAAGAAAGTTAGAGTACAAGCAAGTGAAGGTCACGATCATATAATATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

180

Amino Acids

19.78

Weight (kDa)

5.49

Isoelectric Point (pI)

46.76

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014466)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G59360
fragaria_vesca FvH4_3g35950
malus_domestica MD03G1095400.v1.1 MD11G1110400.v1.1
prunus_persica Prupe.6G078900_v2.0.a1
pyrus_communis pycom03g07830 pycom11g09120
rosa_chinensis RchiOBHm_Chr5g0064301
rosa_laevigata RLG00000035714
rosa_multiflora Rmu_sc0002695.1_g000011
rosa_roxburghii Rroxscaffold_1G00016710
rosa_rugosa Rorug05G0363900
rosa_samantha Rh5AG421600 Rh5BG437400 Rh5CG460000 Rh5DG450600
rosa_wichuraiana Rw5G039700

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 72
AclWI GGATC 1 cut(s) 312
AcoI YGGCCR 1 cut(s) 139
AcsI RAATTY 4 cut(s) 50, 79, 227, 399
AfaI GTAC 2 cut(s) 25, 507
AfiI CCNNNNNNNGG 2 cut(s) 221, 452
AflIII ACRYGT 1 cut(s) 183
AgsI TTSAA 5 cut(s) 55, 79, 85, 202, 286
AjiI CACGTC 1 cut(s) 271
AluBI AGCT 1 cut(s) 153
AluI AGCT 1 cut(s) 153
Alw26I GTCTC 2 cut(s) 61, 129
AlwI GGATC 1 cut(s) 312
AoxI GGCC 1 cut(s) 139
ApoI RAATTY 4 cut(s) 50, 79, 227, 399
AsuHPI GGTGA 1 cut(s) 272
BbsI GAAGAC 2 cut(s) 123, 293
BccI CCATC 4 cut(s) 125, 223, 257, 446
BceAI ACGGC 1 cut(s) 154
BcoDI GTCTC 2 cut(s) 61, 129
BfmI CTRYAG 1 cut(s) 232
BglII AGATCT 1 cut(s) 466
BmgBI CACGTC 1 cut(s) 271
BmiI GGNNCC 1 cut(s) 323
BpiI GAAGAC 2 cut(s) 123, 293
BsaI GGTCTC 1 cut(s) 61
BsaJI CCNNGG 1 cut(s) 45
BsaWI WCCGGW 1 cut(s) 445
Bsc4I CCNNNNNNNGG 2 cut(s) 221, 452
Bse3DI GCAATG 1 cut(s) 335
BseDI CCNNGG 1 cut(s) 45
BseLI CCNNNNNNNGG 2 cut(s) 221, 452
BseMI GCAATG 1 cut(s) 335
BseMII CTCAG 1 cut(s) 168
BseRI GAGGAG 1 cut(s) 173
BshFI GGCC 1 cut(s) 141
BsiSI CCGG 1 cut(s) 446
BslFI GGGAC 2 cut(s) 27, 281
BslI CCNNNNNNNGG 2 cut(s) 221, 452
BsmAI GTCTC 2 cut(s) 61, 129
BsmBI CGTCTC 1 cut(s) 129
BsmFI GGGAC 2 cut(s) 27, 281
BsnI GGCC 1 cut(s) 141
Bso31I GGTCTC 1 cut(s) 61
Bsp143I GATC 4 cut(s) 304, 426, 466, 526
Bsp19I CCATGG 1 cut(s) 45
BspACI CCGC 1 cut(s) 72
BspANI GGCC 1 cut(s) 141
BspCNI CTCAG 2 cut(s) 4, 167
BspHI TCATGA 1 cut(s) 486
BspLI GGNNCC 1 cut(s) 323
BspPI GGATC 1 cut(s) 312
BspTNI GGTCTC 1 cut(s) 61
BsrDI GCAATG 1 cut(s) 335
BssECI CCNNGG 1 cut(s) 45
BssMI GATC 4 cut(s) 304, 426, 466, 526
BssT1I CCWWGG 1 cut(s) 45
Bst4CI ACNGT 1 cut(s) 35
Bst6I CTCTTC 1 cut(s) 368
BstC8I GCNNGC 2 cut(s) 97, 326
BstDEI CTNAG 4 cut(s) 12, 38, 61, 154
BstDSI CCRYGG 1 cut(s) 45
BstKTI GATC 4 cut(s) 307, 429, 469, 529
BstMAI GTCTC 2 cut(s) 61, 129
BstMBI GATC 4 cut(s) 304, 426, 466, 526
BstNSI RCATGY 2 cut(s) 99, 187
BstSFI CTRYAG 1 cut(s) 232
BstV2I GAAGAC 2 cut(s) 123, 293
BstX2I RGATCY 1 cut(s) 466
BstYI RGATCY 1 cut(s) 466
BsuRI GGCC 1 cut(s) 141
BtgI CCRYGG 1 cut(s) 45
BtrI CACGTC 1 cut(s) 271
Cac8I GCNNGC 2 cut(s) 97, 326
CciI TCATGA 1 cut(s) 486
Csp6I GTAC 2 cut(s) 24, 506
CviAII CATG 5 cut(s) 46, 92, 96, 184, 487
CviJI RGCY 3 cut(s) 141, 153, 324
CviKI_1 RGCY 3 cut(s) 141, 153, 324
CviQI GTAC 2 cut(s) 24, 506
DdeI CTNAG 4 cut(s) 12, 38, 61, 154
DpnI GATC 4 cut(s) 306, 428, 468, 528
DpnII GATC 4 cut(s) 304, 426, 466, 526
EaeI YGGCCR 1 cut(s) 139
Eam1104I CTCTTC 1 cut(s) 368
EarI CTCTTC 1 cut(s) 368
Eco130I CCWWGG 1 cut(s) 45
Eco31I GGTCTC 1 cut(s) 61
EcoRI GAATTC 2 cut(s) 50, 227
EcoT14I CCWWGG 1 cut(s) 45
EcoT22I ATGCAT 1 cut(s) 97
ErhI CCWWGG 1 cut(s) 45
Esp3I CGTCTC 1 cut(s) 129
FaeI CATG 5 cut(s) 49, 95, 99, 187, 490
FalI AAGNNNNNCTT 2 cut(s) 372, 404
FaqI GGGAC 2 cut(s) 27, 281
FatI CATG 5 cut(s) 45, 91, 95, 183, 486
FauI CCCGC 1 cut(s) 79
HaeIII GGCC 1 cut(s) 141
HapII CCGG 1 cut(s) 446
Hin1II CATG 5 cut(s) 49, 95, 99, 187, 490
HinfI GANTC 3 cut(s) 237, 289, 431
HpaII CCGG 1 cut(s) 446
HphI GGTGA 1 cut(s) 272
Hpy166II GTNNAC 1 cut(s) 335
Hpy188I TCNGA 2 cut(s) 13, 157
Hpy188III TCNNGA 2 cut(s) 487, 524
Hpy8I GTNNAC 1 cut(s) 335
HpyAV CCTTC 3 cut(s) 196, 242, 512
HpyCH4III ACNGT 1 cut(s) 35
HpyCH4IV ACGT 1 cut(s) 270
HpyCH4V TGCA 3 cut(s) 95, 328, 464
HpyF3I CTNAG 4 cut(s) 12, 38, 61, 154
HpySE526I ACGT 1 cut(s) 270
Hsp92II CATG 5 cut(s) 49, 95, 99, 187, 490
Kzo9I GATC 4 cut(s) 304, 426, 466, 526
LmnI GCTCC 1 cut(s) 321
LpnPI CCDG 7 cut(s) 207, 230, 254, 338, 351, 409, 459
MaeII ACGT 1 cut(s) 270
MaeIII GTNAC 1 cut(s) 521
MalI GATC 4 cut(s) 306, 428, 468, 528
MboI GATC 4 cut(s) 304, 426, 466, 526
MboII GAAGA 9 cut(s) 128, 134, 137, 140, 182, 298, 385, 502, 505
MflI RGATCY 1 cut(s) 466
MluCI AATT 8 cut(s) 50, 55, 79, 102, 143, 175, 227, 399
MlyI GAGTC 2 cut(s) 246, 283
MmeI TCCRAC 1 cut(s) 317
MnlI CCTC 2 cut(s) 151, 453
Mph1103I ATGCAT 1 cut(s) 97
MslI CAYNNNNRTG 1 cut(s) 188
MspI CCGG 1 cut(s) 446
NcoI CCATGG 1 cut(s) 45
NdeII GATC 4 cut(s) 304, 426, 466, 526
NlaIII CATG 5 cut(s) 49, 95, 99, 187, 490
NlaIV GGNNCC 1 cut(s) 323
NmuCI GTSAC 1 cut(s) 521
NsiI ATGCAT 1 cut(s) 97
NspI RCATGY 2 cut(s) 99, 187
PaeI GCATGC 1 cut(s) 99
PagI TCATGA 1 cut(s) 486
PciI ACATGT 1 cut(s) 183
PfeI GAWTC 1 cut(s) 431
PleI GAGTC 2 cut(s) 245, 283
PpsI GAGTC 2 cut(s) 245, 283
PscI ACATGT 1 cut(s) 183
PspN4I GGNNCC 1 cut(s) 323
PsuI RGATCY 1 cut(s) 466
RsaI GTAC 2 cut(s) 25, 507
RsaNI GTAC 2 cut(s) 24, 506
RseI CAYNNNNRTG 1 cut(s) 188
Sau3AI GATC 4 cut(s) 304, 426, 466, 526
SchI GAGTC 2 cut(s) 246, 283
SetI ASST 5 cut(s) 155, 207, 253, 273, 523
SfcI CTRYAG 1 cut(s) 232
SmiMI CAYNNNNRTG 1 cut(s) 188
SphI GCATGC 1 cut(s) 99
Sse9I AATT 8 cut(s) 50, 55, 79, 102, 143, 175, 227, 399
SsiI CCGC 1 cut(s) 72
StyI CCWWGG 1 cut(s) 45
TaaI ACNGT 1 cut(s) 35
TaiI ACGT 1 cut(s) 273
TaqI TCGA 3 cut(s) 180, 416, 429
TasI AATT 8 cut(s) 50, 55, 79, 102, 143, 175, 227, 399
TatI WGTACW 1 cut(s) 505
TfiI GAWTC 1 cut(s) 431
TseFI GTSAC 1 cut(s) 521
Tsp45I GTSAC 1 cut(s) 521
TspDTI ATGAA 1 cut(s) 503
XapI RAATTY 4 cut(s) 50, 79, 227, 399
XceI RCATGY 2 cut(s) 99, 187
Zsp2I ATGCAT 1 cut(s) 97
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.