Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr03

Orthogroup ID

OG0001921

Physical Location & Seq

Genomic Coordinates Reverse (-)

11448245 .. 11455437

Gene Structure 7193 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD03G1122400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2424 bp

ATGGAGATGAGTGGCTTTAGGCATGAATCACATGTAGCACAACAAAGTCGGCGAGATAAGTTGAGAGTTAATCAAACTTCAAGCCCACCTCACCATCTTGACAATTTAGCAAACAATTCTGGGAAGTTGCCACTCCATCCAGGACTAAACCCAGATATCGTGCAAGTTCGAAATGTGAGGAATGCGAATTTGCTCTATGATCCAACTATGTTTTCTTCTGAAATGCTCGATTTTTCAATCAATGCAAATGCTCCGTTGTCAGCTCACAGAATCACATTGGCATGTCATGATCAAGATCTAGGGGCAGCTCAACCCGCCGGCGGCGAGAGTCAGAATTTTGGTAATTGGAGAAGTACTCTTAATCCACCTCAAAGCATAGATTGGGTGAATAATTACACAAGTGGATATCAGGATGTAGTTCAGTCTAGTTTGGCCAACTCATCTGCTGATCAAATTTCTAGTCATCATGTTGCCCAAAAGCACTTGGGAGGAGGAACAATGCACTTTTCGTCCCCTTCGATAAAATTCGTTAACACTCTCCAAGATGTTGTCACTTCAGCTTCTCAAGGGACGCAGCAGGATCAGCTAGAAATGGCTTCAATTGTGCACCAAAGAATCATGGAAAATGAACTTGTTCGCGTTCCAAGCTACGGGAATCAATCGAACACATTGCGCTTCAATAGTGCGAGTAATTCTTGGATGGATCGGCAGCCTATCGAGAATCGCCATAATTGGAGTAGTGGTGGTGGCGGGGGACTAGAATTTAGTACTGCAAACAGTGTTGATGAAGAAATGAGGACCGGTATGAGTAGCGATTCCAACCAACAAGGGCTGTCTCTATCACTCTCATCAAATCCACCATCTAATAAACTGCCTGTGGCTCAATTAATTGGGTCTCAAGATTTACATGCGAGCACTGATGATGATGATGATCATCATCATCAAGCACTTAAGGATCTTCAAAATTCGAAAATGGGGAAATCTTCCGGTGGATACTTGTGTTCCATAACAAAGCCATCTATTATTAGCAAAACTTGTGGAAAATCACTTAAAGACATTGTGGGGAATTCTAGTACTAGCGCCTATCGAAGCACTGGTCCTCTTGGTCCTTTCACTGGATATGCAACGATTCTGAAGAGTTCGAAGTTCTTGAAACCGGCTCAACAGCTACTGGATGAATTCTGTTGGGTTAGTGATTCAAAGATACTAAAAACATGTGAGGCATCTGAAAGGATGTCTGGAGATGTGAGTGCTTCGGCCTCGGTTTCAATTTCCATTGAAGCTGGTAATGCAACCGAAACTGAAGTAGTGGCCAAGAGGAACAACTCCGGTGCATCTTCCTCTACGTTTTACGGTTCAACGGAAATTAGCAGTGATGGTGGAGCTGCAAGCATATCTTCTGAGTCCTTTTGGCCGGAGTATCAACAAAAGAAAGCAAAGCTCCTATATATGCAGGAGGAGGTTAGCAGAAGGTACAAGCAATATCATCAACAAATGCAAATGGTGGTTGCTTCCTTCGAATCAGTTTCCGGTCTGAGTTCCGCTACGCCGTACATATGCATGGCCCTAAACGCAGTCTCAAGACACTTCAGGTGTCTTACAAATTCCATCAAGGACCAGCTCAAGCACACAAGGAAGGCTTTGGGAGAGGAATATATGTCATCCTCTGTAACTGCTGCTGGTACTGCTGAGTGCAGTAGTAAAGGTGAGAAAAATTTCGCCAAGCTAAAGTATATGGGCCTAGGTTTCCAAAAGCATAATAAGTCTGGCGGTGGGTCCCTTGTGGGTTTCTCTGAACCCCAACAACACGTCTGGAGACCCCAGAGAGGCCTACCTGAACGTTCAGTAGCCATTCTCAGAACTTGGCTCTTCGAGCATTTTCTTCACCCGTATCCTACTGACACAGATAAGCACATGTTAGCCACTCAAACGGGTCTATCTCGAAACCAGGTGTCAAACTGGTTCATAAACGCCCGAGTGCGCGTTTGGAAGCCCATGGTTGAAGAAATACACATGCTCGAGACCAGAGGAGGCTCCTTCGAAGCAGGCCAAGATCCTATCATTAAGGATGGAAATTCTGCAAATGAAGGCACCAGCAGCAGGCCAAACAATGATCACCAACTAAGCATGAATGTGATGCCCAATAGACAATTAGAGTGCTCAGTTGATGCAGAGCAACGAAATCAGGAGATGAAGAGGTCTAGATTGGAGTGCCAAGTTCCATCAAACATGGACGGAGGATTAATGGGTTTTGTCCAGGACCAGCGAGGAGGGTTTGAGGCTGGGGGACTTGGAGCCGTGTCCCTTACTTTGGGTCTCAGACATGGGGTGGAAAGTGCCCAGCAGCAGCAACAACTGCAGCAACAAGAGGATCAGCTTCGGCGGCAACTTGGAGGTCAAATGATACGTGATTATGCGGGGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0001763 GO:0003006 GO:0003674 GO:0003700 GO:0006355 GO:0007275 GO:0007389 GO:0008150 GO:0009653 GO:0009791 GO:0009888 GO:0009889 GO:0010016 GO:0010073 GO:0010074 GO:0010076 GO:0010077 GO:0010223 GO:0010228 GO:0010229 GO:0010346 GO:0010468 GO:0010556 GO:0019219 GO:0019222 GO:0019827 GO:0022414 GO:0031323 GO:0031326 GO:0032501 GO:0032502 GO:0048367 GO:0048507 GO:0048608 GO:0048646 GO:0048731 GO:0048856 GO:0050789 GO:0050794 GO:0051171 GO:0051252 GO:0060255 GO:0061458 GO:0065007 GO:0080006 GO:0080090 GO:0090567 GO:0098727 GO:0140110 GO:1903506 GO:1905393 GO:2000112 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Homeobox_KN POX

Protein Analysis

808

Amino Acids

88.57

Weight (kDa)

6.64

Isoelectric Point (pI)

54.14

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
POX	PF07526	377 - 537	1.1e-38	Associated with HOX
Homeobox_KN	PF05920	622 - 661	2.2e-19	Homeobox KN domain
Homeodomain	PF00046	629 - 661	2.4e-06	Homeodomain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001921)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G27990 AT2G27990
fragaria_vesca	FvH4_3g31640 FvH4_3g31640 FvH4_3g31640 FvH4_3g31640 FvH4_3g31640 FvH4_3g31640 FvH4_3g31640 FvH4_3g31640 FvH4_3g31640 FvH4_3g31640 FvH4_3g31640 FvH4_3g31640 FvH4_3g31640 FvH4_3g31640 FvH4_3g31640 FvH4_3g31640 FvH4_3g31640 FvH4_3g31640 FvH4_3g31640 FvH4_3g31640 FvH4_3g31640 FvH4_3g31640 FvH4_3g31640 FvH4_3g31640
malus_domestica	MD03G1122400.v1.1 MD11G1140600.v1.1
prunus_persica	Prupe.6G105100_v2.0.a1 Prupe.6G105100_v2.0.a1 Prupe.6G105100_v2.0.a1 Prupe.6G105100_v2.0.a1 Prupe.6G105100_v2.0.a1 Prupe.6G105100_v2.0.a1
pyrus_communis	pycom11g11630
rosa_chinensis	RchiOBHm_Chr5g0058351
rosa_laevigata	RLG00000035214
rosa_roxburghii	Rroxscaffold_1G00022230
rosa_rugosa	Rorug05G0313100 Rorug05G0313200 Rorug05G0313300
rosa_samantha	Rh5AG380800 Rh5BG392300 Rh5CG416300 Rh5DG407200
rosa_wichuraiana	Rw5G035880

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	1 cut(s)	2090
AccII	CGCG	2 cut(s)	639, 1983
AciI	CCGC	7 cut(s)	315, 321, 750, 1542, 1770, 2383, 2417
AclI	AACGTT	1 cut(s)	1840
AclWI	GGATC	6 cut(s)	194, 588, 711, 963, 2048, 2379
AcoI	YGGCCR	3 cut(s)	432, 1311, 1412
AcuI	CTGAAG	4 cut(s)	540, 1154, 1323, 1573
AfaI	GTAC	6 cut(s)	355, 769, 1075, 1475, 1553, 1684
AfiI	CCNNNNNNNGG	6 cut(s)	320, 650, 1115, 1826, 2100, 2311
AflII	CTTAAG	1 cut(s)	950
AflIII	ACRYGT	4 cut(s)	31, 1214, 1807, 1914
AgeI	ACCGGT	1 cut(s)	800
AjiI	CACGTC	1 cut(s)	1810
AjnI	CCWGG	3 cut(s)	139, 1947, 2256
AjuI	GAANNNNNNNTTGG	2 cut(s)	196, 228
Alw21I	GWGCWC	3 cut(s)	609, 917, 2162
Alw26I	GTCTC	6 cut(s)	840, 900, 1582, 1810, 2015, 2321
Alw44I	GTGCAC	1 cut(s)	605
AlwI	GGATC	6 cut(s)	194, 588, 711, 963, 2048, 2379
AlwNI	CAGNNNCTG	1 cut(s)	1171
Ama87I	CYCGRG	2 cut(s)	1974, 2018
AoxI	GGCC	9 cut(s)	432, 1257, 1311, 1412, 1563, 1738, 1828, 2047, 2102
ApaLI	GTGCAC	1 cut(s)	605
ApeKI	GCWGC	9 cut(s)	305, 574, 709, 1385, 1676, 2097, 2344, 2347, 2359
ArsI	GACNNNNNNTTYG	4 cut(s)	445, 477, 1081, 1113
AseI	ATTAAT	2 cut(s)	887, 2243
AsiGI	ACCGGT	1 cut(s)	800
Asp700I	GAANNNNTTC	2 cut(s)	633, 1715
AspA2I	CCTAGG	1 cut(s)	1741
AspLEI	GCGC	3 cut(s)	675, 1082, 1983
AspS9I	GGNCC	8 cut(s)	798, 1097, 1106, 1564, 1615, 1738, 1776, 2260
AsuHPI	GGTGA	5 cut(s)	83, 397, 1718, 1877, 2108
AsuII	TTCGAA	5 cut(s)	169, 968, 1142, 1518, 2040
AvaI	CYCGRG	2 cut(s)	1974, 2018
AvaII	GGWCC	6 cut(s)	798, 1097, 1106, 1615, 1776, 2260
AvrII	CCTAGG	1 cut(s)	1741
BaeGI	GKGCMC	2 cut(s)	609, 2341
BaeI	ACNNNNGTAYC	2 cut(s)	985, 1018
BalI	TGGCCA	2 cut(s)	434, 1313
BanI	GGYRCC	1 cut(s)	2090
Bbv12I	GWGCWC	3 cut(s)	609, 917, 2162
BbvI	GCAGC	9 cut(s)	317, 586, 721, 1372, 1663, 2109, 2356, 2359, 2371
BccI	CCATC	9 cut(s)	102, 144, 694, 868, 1024, 1370, 1616, 2063, 2230
BceAI	ACGGC	2 cut(s)	1534, 2282
BcgI	CGANNNNNNTGC	2 cut(s)	652, 686
BciT130I	CCWGG	3 cut(s)	141, 1949, 2258
BciVI	GTATCC	2 cut(s)	986, 1902
BclI	TGATCA	4 cut(s)	289, 448, 931, 2113
BcoDI	GTCTC	6 cut(s)	840, 900, 1582, 1810, 2015, 2321
BfaI	CTAG	9 cut(s)	299, 426, 459, 587, 758, 1071, 1077, 1742, 2202
BfmI	CTRYAG	1 cut(s)	2357
BfoI	RGCGCY	1 cut(s)	1083
BfrI	CTTAAG	1 cut(s)	950
BfuI	GTATCC	2 cut(s)	986, 1902
BglII	AGATCT	1 cut(s)	295
BlnI	CCTAGG	1 cut(s)	1741
BmcAI	AGTACT	3 cut(s)	355, 769, 1075
Bme1390I	CCNGG	3 cut(s)	141, 1949, 2258
Bme18I	GGWCC	6 cut(s)	798, 1097, 1106, 1615, 1776, 2260
BmeT110I	CYCGRG	2 cut(s)	1974, 2018
BmgBI	CACGTC	1 cut(s)	1810
BmgT120I	GGNCC	8 cut(s)	798, 1097, 1106, 1564, 1615, 1738, 1776, 2260
BmiI	GGNNCC	5 cut(s)	1777, 1778, 2035, 2092, 2296
BmrFI	CCNGG	3 cut(s)	141, 1949, 2258
BmsI	GCATC	4 cut(s)	1232, 1343, 2127, 2158
BplI	GAGNNNNNCTC	2 cut(s)	340, 372
BpmI	CTGGAG	2 cut(s)	1260, 1834
Bpu14I	TTCGAA	5 cut(s)	169, 968, 1142, 1518, 2040
BpuEI	CTTGAG	4 cut(s)	549, 882, 1564, 1607
BsaAI	YACGTR	1 cut(s)	2408
BsaBI	GATNNNNATC	4 cut(s)	294, 930, 933, 936
BsaI	GGTCTC	4 cut(s)	900, 1810, 2015, 2321
BsaJI	CCNNGG	3 cut(s)	1260, 1741, 1995
BsaWI	WCCGGW	4 cut(s)	800, 986, 1328, 1529
BsaXI	ACNNNNNCTCC	2 cut(s)	727, 757
Bsc4I	CCNNNNNNNGG	6 cut(s)	320, 650, 1115, 1826, 2100, 2311
Bse118I	RCCGGY	3 cut(s)	317, 800, 1156
Bse1I	ACTGG	4 cut(s)	1099, 1120, 1176, 1964
Bse3DI	GCAATG	1 cut(s)	668
Bse8I	GATNNNNATC	4 cut(s)	294, 930, 933, 936
BseBI	CCWGG	3 cut(s)	141, 1949, 2258
BseDI	CCNNGG	3 cut(s)	1260, 1741, 1995
BseGI	GGATG	7 cut(s)	136, 418, 705, 1180, 1239, 1661, 2074
BseJI	GATNNNNATC	4 cut(s)	294, 930, 933, 936
BseLI	CCNNNNNNNGG	6 cut(s)	320, 650, 1115, 1826, 2100, 2311
BseMI	GCAATG	1 cut(s)	668
BseMII	CTCAG	6 cut(s)	1392, 1526, 1680, 1870, 2175, 2332
BseNI	ACTGG	4 cut(s)	1099, 1120, 1176, 1964
BseRI	GAGGAG	4 cut(s)	504, 1472, 2043, 2283
BseSI	GKGCMC	2 cut(s)	609, 2341
BseXI	GCAGC	9 cut(s)	317, 586, 721, 1372, 1663, 2109, 2356, 2359, 2371
BseYI	CCCAGC	2 cut(s)	2282, 2340
BsgI	GTGCAG	1 cut(s)	1714
Bsh1236I	CGCG	2 cut(s)	639, 1983
BshFI	GGCC	9 cut(s)	434, 1259, 1313, 1414, 1565, 1740, 1830, 2049, 2104
BshNI	GGYRCC	1 cut(s)	2090
BshTI	ACCGGT	1 cut(s)	800
BsiHKAI	GWGCWC	3 cut(s)	609, 917, 2162
BsiHKCI	CYCGRG	2 cut(s)	1974, 2018
BsiSI	CCGG	7 cut(s)	318, 801, 987, 1157, 1329, 1415, 1530
BslFI	GGGAC	6 cut(s)	496, 583, 768, 1762, 2287, 2301
BslI	CCNNNNNNNGG	6 cut(s)	320, 650, 1115, 1826, 2100, 2311
BsmAI	GTCTC	6 cut(s)	840, 900, 1582, 1810, 2015, 2321
BsmFI	GGGAC	6 cut(s)	496, 583, 768, 1762, 2287, 2301
BsmI	GAATGC	1 cut(s)	187
BsnI	GGCC	9 cut(s)	434, 1259, 1313, 1414, 1565, 1740, 1830, 2049, 2104
Bso31I	GGTCTC	4 cut(s)	900, 1810, 2015, 2321
BsoBI	CYCGRG	2 cut(s)	1974, 2018
Bsp119I	TTCGAA	5 cut(s)	169, 968, 1142, 1518, 2040
Bsp1286I	GDGCHC	4 cut(s)	609, 917, 2162, 2341
Bsp19I	CCATGG	1 cut(s)	1995
BspACI	CCGC	7 cut(s)	315, 321, 750, 1542, 1770, 2383, 2417
BspANI	GGCC	9 cut(s)	434, 1259, 1313, 1414, 1565, 1740, 1830, 2049, 2104
BspCNI	CTCAG	6 cut(s)	1393, 1527, 1681, 1869, 2174, 2331
BspFNI	CGCG	2 cut(s)	639, 1983
BspHI	TCATGA	1 cut(s)	286
BspLI	GGNNCC	5 cut(s)	1777, 1778, 2035, 2092, 2296
BspMAI	CTGCAG	1 cut(s)	2361
BspPI	GGATC	6 cut(s)	194, 588, 711, 963, 2048, 2379
BspQI	GCTCTTC	1 cut(s)	1874
BspT104I	TTCGAA	5 cut(s)	169, 968, 1142, 1518, 2040
BspT107I	GGYRCC	1 cut(s)	2090
BspTI	CTTAAG	1 cut(s)	950
BspTNI	GGTCTC	4 cut(s)	900, 1810, 2015, 2321
BsrDI	GCAATG	1 cut(s)	668
BsrFI	RCCGGY	3 cut(s)	317, 800, 1156
BsrI	ACTGG	4 cut(s)	1099, 1120, 1176, 1964
BssAI	RCCGGY	3 cut(s)	317, 800, 1156
BssECI	CCNNGG	3 cut(s)	1260, 1741, 1995
BssT1I	CCWWGG	2 cut(s)	1741, 1995
Bst2UI	CCWGG	3 cut(s)	141, 1949, 2258
Bst4CI	ACNGT	2 cut(s)	779, 1355
Bst6I	CTCTTC	3 cut(s)	1130, 1874, 2189
BstAFI	CTTAAG	1 cut(s)	950
BstAPI	GCANNNNNTGC	1 cut(s)	2356
BstBAI	YACGTR	1 cut(s)	2408
BstBI	TTCGAA	5 cut(s)	169, 968, 1142, 1518, 2040
BstC8I	GCNNGC	5 cut(s)	319, 913, 1390, 2047, 2102
BstDEI	CTNAG	7 cut(s)	1401, 1535, 1689, 1856, 2123, 2161, 2318
BstDSI	CCRYGG	1 cut(s)	1995
BstF5I	GGATG	7 cut(s)	136, 418, 705, 1180, 1239, 1661, 2074
BstFNI	CGCG	2 cut(s)	639, 1983
BstH2I	RGCGCY	1 cut(s)	1083
BstHHI	GCGC	3 cut(s)	675, 1082, 1983
BstMAI	GTCTC	6 cut(s)	840, 900, 1582, 1810, 2015, 2321
BstNI	CCWGG	3 cut(s)	141, 1949, 2258
BstNSI	RCATGY	6 cut(s)	35, 285, 911, 1218, 1918, 2017
BstSCI	CCNGG	3 cut(s)	139, 1947, 2256
BstSFI	CTRYAG	1 cut(s)	2357
BstSLI	GKGCMC	2 cut(s)	609, 2341
BstUI	CGCG	2 cut(s)	639, 1983
BstV1I	GCAGC	9 cut(s)	317, 586, 721, 1372, 1663, 2109, 2356, 2359, 2371
BstX2I	RGATCY	3 cut(s)	295, 955, 2053
BstYI	RGATCY	3 cut(s)	295, 955, 2053
BsuI	GTATCC	2 cut(s)	986, 1902
BsuRI	GGCC	9 cut(s)	434, 1259, 1313, 1414, 1565, 1740, 1830, 2049, 2104
BtgI	CCRYGG	1 cut(s)	1995
BtrI	CACGTC	1 cut(s)	1810
BtsCI	GGATG	7 cut(s)	136, 418, 705, 1180, 1239, 1661, 2074
BtsI	GCAGTG	1 cut(s)	1378
BtsIMutI	CAGTG	5 cut(s)	784, 915, 1092, 1113, 1378
Cac8I	GCNNGC	5 cut(s)	319, 913, 1390, 2047, 2102
CaiI	CAGNNNCTG	1 cut(s)	1171
CciI	TCATGA	1 cut(s)	286
CfoI	GCGC	3 cut(s)	675, 1082, 1983
Cfr10I	RCCGGY	3 cut(s)	317, 800, 1156
Cfr13I	GGNCC	8 cut(s)	798, 1097, 1106, 1564, 1615, 1738, 1776, 2260
CseI	GACGC	1 cut(s)	580
CsiI	ACCWGGT	1 cut(s)	1947
Csp6I	GTAC	6 cut(s)	354, 768, 1074, 1474, 1552, 1683
CspAI	ACCGGT	1 cut(s)	800
CspCI	CAANNNNNGTGG	2 cut(s)	1018, 1053
CviQI	GTAC	6 cut(s)	354, 768, 1074, 1474, 1552, 1683
DdeI	CTNAG	7 cut(s)	1401, 1535, 1689, 1856, 2123, 2161, 2318
EaeI	YGGCCR	3 cut(s)	432, 1311, 1412
Eam1104I	CTCTTC	3 cut(s)	1130, 1874, 2189
EarI	CTCTTC	3 cut(s)	1130, 1874, 2189
Eco130I	CCWWGG	2 cut(s)	1741, 1995
Eco147I	AGGCCT	1 cut(s)	1830
Eco31I	GGTCTC	4 cut(s)	900, 1810, 2015, 2321
Eco32I	GATATC	2 cut(s)	157, 407
Eco47I	GGWCC	6 cut(s)	798, 1097, 1106, 1615, 1776, 2260
Eco57I	CTGAAG	4 cut(s)	540, 1154, 1323, 1573
Eco88I	CYCGRG	2 cut(s)	1974, 2018
EcoO109I	RGGNCCY	1 cut(s)	1776
EcoRI	GAATTC	2 cut(s)	1066, 1178
EcoRII	CCWGG	3 cut(s)	139, 1947, 2256
EcoRV	GATATC	2 cut(s)	157, 407
EcoT14I	CCWWGG	2 cut(s)	1741, 1995
EcoT22I	ATGCAT	1 cut(s)	1562
ErhI	CCWWGG	2 cut(s)	1741, 1995
FalI	AAGNNNNNCTT	4 cut(s)	1381, 1413, 1624, 1656
FaqI	GGGAC	6 cut(s)	496, 583, 768, 1762, 2287, 2301
FauI	CCCGC	3 cut(s)	322, 743, 2410
FauNDI	CATATG	1 cut(s)	1556
FbaI	TGATCA	4 cut(s)	289, 448, 931, 2113
FokI	GGATG	7 cut(s)	123, 425, 712, 1187, 1246, 1648, 2081
FspBI	CTAG	9 cut(s)	299, 426, 459, 587, 758, 1071, 1077, 1742, 2202
GlaI	GCGC	3 cut(s)	674, 1081, 1982
GsaI	CCCAGC	2 cut(s)	2286, 2344
GsuI	CTGGAG	2 cut(s)	1260, 1834
HaeII	RGCGCY	1 cut(s)	1083
HaeIII	GGCC	9 cut(s)	434, 1259, 1313, 1414, 1565, 1740, 1830, 2049, 2104
HapII	CCGG	7 cut(s)	318, 801, 987, 1157, 1329, 1415, 1530
HgaI	GACGC	1 cut(s)	580
HhaI	GCGC	3 cut(s)	675, 1082, 1983
Hin6I	GCGC	3 cut(s)	673, 1080, 1981
HinP1I	GCGC	3 cut(s)	673, 1080, 1981
HincII	GTYRAC	1 cut(s)	532
HindII	GTYRAC	1 cut(s)	532
HpaI	GTTAAC	1 cut(s)	532
HpaII	CCGG	7 cut(s)	318, 801, 987, 1157, 1329, 1415, 1530
HphI	GGTGA	5 cut(s)	83, 397, 1718, 1877, 2108
Hpy166II	GTNNAC	2 cut(s)	532, 607
Hpy188I	TCNGA	9 cut(s)	220, 333, 1134, 1228, 1402, 1536, 1795, 1859, 2321
Hpy8I	GTNNAC	2 cut(s)	532, 607
HpyAV	CCTTC	6 cut(s)	525, 1464, 1525, 1630, 2047, 2081
HpyCH4III	ACNGT	2 cut(s)	779, 1355
HpyCH4IV	ACGT	4 cut(s)	1346, 1809, 1840, 2407
HpyF3I	CTNAG	7 cut(s)	1401, 1535, 1689, 1856, 2123, 2161, 2318
HpySE526I	ACGT	4 cut(s)	1346, 1809, 1840, 2407
HspAI	GCGC	3 cut(s)	673, 1080, 1981
KflI	GGGWCCC	1 cut(s)	1776
KroI	GCCGGC	1 cut(s)	317
KroNI	GCCGGC	1 cut(s)	319
Ksp22I	TGATCA	4 cut(s)	289, 448, 931, 2113
KspAI	GTTAAC	1 cut(s)	532
LguI	GCTCTTC	1 cut(s)	1874
LmnI	GCTCC	5 cut(s)	256, 1382, 1446, 2039, 2294
Lsp1109I	GCAGC	9 cut(s)	317, 586, 721, 1372, 1663, 2109, 2356, 2359, 2371
LweI	GCATC	4 cut(s)	1232, 1343, 2127, 2158
MabI	ACCWGGT	1 cut(s)	1947
MaeI	CTAG	9 cut(s)	299, 426, 459, 587, 758, 1071, 1077, 1742, 2202
MaeII	ACGT	4 cut(s)	1346, 1809, 1840, 2407
MaeIII	GTNAC	2 cut(s)	550, 1669
MfeI	CAATTG	1 cut(s)	600
MflI	RGATCY	3 cut(s)	295, 955, 2053
MhlI	GDGCHC	4 cut(s)	609, 917, 2162, 2341
MlsI	TGGCCA	2 cut(s)	434, 1313
MluNI	TGGCCA	2 cut(s)	434, 1313
MlyI	GAGTC	2 cut(s)	337, 1412
MmeI	TCCRAC	2 cut(s)	227, 843
Mox20I	TGGCCA	2 cut(s)	434, 1313
Mph1103I	ATGCAT	1 cut(s)	1562
MreI	CGCCGGCG	1 cut(s)	317
MroNI	GCCGGC	1 cut(s)	317
MroXI	GAANNNNTTC	2 cut(s)	633, 1715
MscI	TGGCCA	2 cut(s)	434, 1313
MseI	TTAA	8 cut(s)	69, 360, 531, 887, 951, 1050, 2064, 2243
MslI	CAYNNNNRTG	3 cut(s)	280, 1559, 2132
Msp20I	TGGCCA	2 cut(s)	434, 1313
MspCI	CTTAAG	1 cut(s)	950
MspI	CCGG	7 cut(s)	318, 801, 987, 1157, 1329, 1415, 1530
MspR9I	CCNGG	3 cut(s)	141, 1949, 2258
MunI	CAATTG	1 cut(s)	600
Mva1269I	GAATGC	1 cut(s)	187
MvaI	CCWGG	3 cut(s)	141, 1949, 2258
MvnI	CGCG	2 cut(s)	639, 1983
NaeI	GCCGGC	1 cut(s)	319
NcoI	CCATGG	1 cut(s)	1995
NdeI	CATATG	1 cut(s)	1556
NgoMIV	GCCGGC	1 cut(s)	317
NlaIV	GGNNCC	5 cut(s)	1777, 1778, 2035, 2092, 2296
NmuCI	GTSAC	1 cut(s)	550
NsiI	ATGCAT	1 cut(s)	1562
NspI	RCATGY	6 cut(s)	35, 285, 911, 1218, 1918, 2017
NspV	TTCGAA	5 cut(s)	169, 968, 1142, 1518, 2040
PaeR7I	CTCGAG	1 cut(s)	2018
PagI	TCATGA	1 cut(s)	286
PceI	AGGCCT	1 cut(s)	1830
PciI	ACATGT	3 cut(s)	31, 1214, 1914
PciSI	GCTCTTC	1 cut(s)	1874
PcsI	WCGNNNNNNNCGW	1 cut(s)	515
PctI	GAATGC	1 cut(s)	187
PdiI	GCCGGC	1 cut(s)	319
PdmI	GAANNNNTTC	2 cut(s)	633, 1715
PfeI	GAWTC	9 cut(s)	26, 270, 615, 655, 721, 815, 1129, 1196, 1520
PfoI	TCCNGGA	2 cut(s)	139, 2256
PinAI	ACCGGT	1 cut(s)	800
PleI	GAGTC	2 cut(s)	336, 1411
PpsI	GAGTC	2 cut(s)	336, 1411
Ppu21I	YACGTR	1 cut(s)	2408
PpuMI	RGGWCCY	1 cut(s)	1776
PscI	ACATGT	3 cut(s)	31, 1214, 1914
PshBI	ATTAAT	2 cut(s)	887, 2243
Psp1406I	AACGTT	1 cut(s)	1840
Psp5II	RGGWCCY	1 cut(s)	1776
Psp6I	CCWGG	3 cut(s)	139, 1947, 2256
PspFI	CCCAGC	2 cut(s)	2282, 2340
PspGI	CCWGG	3 cut(s)	139, 1947, 2256
PspN4I	GGNNCC	5 cut(s)	1777, 1778, 2035, 2092, 2296
PspPI	GGNCC	8 cut(s)	798, 1097, 1106, 1564, 1615, 1738, 1776, 2260
PspPPI	RGGWCCY	1 cut(s)	1776
PstI	CTGCAG	1 cut(s)	2361
PstNI	CAGNNNCTG	1 cut(s)	1171
PsuI	RGATCY	3 cut(s)	295, 955, 2053
RsaI	GTAC	6 cut(s)	355, 769, 1075, 1475, 1553, 1684
RsaNI	GTAC	6 cut(s)	354, 768, 1074, 1474, 1552, 1683
RseI	CAYNNNNRTG	3 cut(s)	280, 1559, 2132
SapI	GCTCTTC	1 cut(s)	1874
SaqAI	TTAA	8 cut(s)	69, 360, 531, 887, 951, 1050, 2064, 2243
Sau96I	GGNCC	8 cut(s)	798, 1097, 1106, 1564, 1615, 1738, 1776, 2260
ScaI	AGTACT	3 cut(s)	355, 769, 1075
SchI	GAGTC	2 cut(s)	337, 1412
ScrFI	CCNGG	3 cut(s)	141, 1949, 2258
SduI	GDGCHC	4 cut(s)	609, 917, 2162, 2341
SexAI	ACCWGGT	1 cut(s)	1947
SfaNI	GCATC	4 cut(s)	1232, 1343, 2127, 2158
SfcI	CTRYAG	1 cut(s)	2357
Sfr274I	CTCGAG	1 cut(s)	2018
SfuI	TTCGAA	5 cut(s)	169, 968, 1142, 1518, 2040
SgrAI	CRCCGGYG	1 cut(s)	317
SinI	GGWCC	6 cut(s)	798, 1097, 1106, 1615, 1776, 2260
SlaI	CTCGAG	1 cut(s)	2018
SmiMI	CAYNNNNRTG	3 cut(s)	280, 1559, 2132
SmlI	CTYRAG	6 cut(s)	564, 897, 950, 1579, 1622, 2018
SmoI	CTYRAG	6 cut(s)	564, 897, 950, 1579, 1622, 2018
SseBI	AGGCCT	1 cut(s)	1830
SsiI	CCGC	7 cut(s)	315, 321, 750, 1542, 1770, 2383, 2417
SspMI	CTAG	9 cut(s)	299, 426, 459, 587, 758, 1071, 1077, 1742, 2202
StuI	AGGCCT	1 cut(s)	1830
StyD4I	CCNGG	3 cut(s)	139, 1947, 2256
StyI	CCWWGG	2 cut(s)	1741, 1995
TaaI	ACNGT	2 cut(s)	779, 1355
TaiI	ACGT	4 cut(s)	1349, 1812, 1843, 2410
TatI	WGTACW	3 cut(s)	353, 767, 1073
TauI	GCSGC	2 cut(s)	324, 2386
TfiI	GAWTC	9 cut(s)	26, 270, 615, 655, 721, 815, 1129, 1196, 1520
Tru1I	TTAA	8 cut(s)	69, 360, 531, 887, 951, 1050, 2064, 2243
Tru9I	TTAA	8 cut(s)	69, 360, 531, 887, 951, 1050, 2064, 2243
TscAI	CASTG	5 cut(s)	784, 922, 1099, 1120, 1378
TseFI	GTSAC	1 cut(s)	550
TseI	GCWGC	9 cut(s)	305, 574, 709, 1385, 1676, 2097, 2344, 2347, 2359
Tsp45I	GTSAC	1 cut(s)	550
TspDTI	ATGAA	8 cut(s)	39, 642, 801, 1191, 1954, 2100, 2144, 2207
TspGWI	ACGGA	3 cut(s)	243, 1376, 2250
TspRI	CASTG	5 cut(s)	784, 922, 1099, 1120, 1378
Vha464I	CTTAAG	1 cut(s)	950
VneI	GTGCAC	1 cut(s)	605
VpaK11BI	GGWCC	6 cut(s)	798, 1097, 1106, 1615, 1776, 2260
VspI	ATTAAT	2 cut(s)	887, 2243
XbaI	TCTAGA	1 cut(s)	2201
XceI	RCATGY	6 cut(s)	35, 285, 911, 1218, 1918, 2017
XhoI	CTCGAG	1 cut(s)	2018
XmaJI	CCTAGG	1 cut(s)	1741
XmnI	GAANNNNTTC	2 cut(s)	633, 1715
XspI	CTAG	9 cut(s)	299, 426, 459, 587, 758, 1071, 1077, 1742, 2202
ZrmI	AGTACT	3 cut(s)	355, 769, 1075
Zsp2I	ATGCAT	1 cut(s)	1562

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.