Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr5D

Orthogroup ID

OG0001921

Physical Location & Seq

Genomic Coordinates Forward (+)

64408825 .. 64414207

Gene Structure 5383 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh5DG407200.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2289 bp

ATGGAGATGAGTGGCTTTAGGCCTAGTGAGTCCACCCATGTAGCTCAGCAAAGCCGCCGTGATAAGTTGCGAGTTCAACAAACATCAAACCTATCCCATCATCATTTAGATCATAATGTACCGAACAATTCACCAACCCACCAAAACCCAGATATTGTTCAAGTTCGAAATGTCAGAAATGCGAACAATCTACTTTTTGATCCGACCCTCTTCTCCCCAGAACTTCTCAATTTTTCGATAAATGCAAATCATGCATTTGCATCATCAGCTCAAGATTTAGGTGACGAGAGTCCTCAGAATTATGGTAATTGGAGAAATCTAAACCCACCCCAAAGCTTAGATAATTGGGTTACCAATTACACGAGTGGTACTACTTCAAACCACCATATGTTTGTTAGGGAAGTAAGTAACATTTCTCCATCCACACCATCAAATTTAAAGCCTGGTACTTACCATGGCTACCAGCAATTTTCTTCTCCGCCTCCAATTTACCATAATACCCTCCAAGATGTTGTCACTACAGCTTCCACCGGAGGAAGCCAGCAGGATCAGCTAGAAATGGCTTCATGGACTCAAGGCTATGTAAATCAATCCACTACATTGGGATTCGATAACTCGAGTTCTTGGATGGATCGTCCAATTGGGAGTAATCGCCATAACTGGAGCGGGGGCGAAGAATTGAGATGTGCTATGAGTGATTATTCCAATCAACAAGGCCTGTCTCTATCACTTTCTTCAAATCCTCCACCTTCACAGCAATTGCAAGCAAGTACTACTGGGAAATCTGGCGGTTACTTATGTTCGATGATGAAGCCATCAATTATAAGCAAAGCGTATGGAACTAGCAAATCACTTCAAGACATAGTTGGGACTAGTGCTACTTCTACTAGTAATGCTGCATATCATAGAAGCACGGGTCCTCTTGGTCCTTTCACTGGATATGCAACGATTTTGAAGAGTTCCAAGTTCTTGAAACCGGCACAACAGCTGTTGGAAGAATTCTGTAGAGCTAGTAATTCGAAAGAATTCAAAACGTGCGAGGCGTCTGAGAGGATATCTGGGGATCAAGCCAGTGCTTCTGCTTCTGTTTCCGTTACAGTCTCGAATGATCAAGCTGCTAATACAACTCAAAATTCCGTAGTTGCTAATATGGGGAGAAATAATTCTGGTGCATCATCCTCTGCATTTTACGGTTCGAATGAAATTAGCAGTGATGGTGGAGCTGCTGCTGCAAGCACGTCTTCTGAGTCGTTTAGGCCGGAGTACCAACAGAAGAAGGCAAAGCTCCTATGCATGCAGGAGGAGGTATGCAGAAAGTACAAGCAATATCATCAACAAATGGAAATGGTGGTCTCTTCCTTTGAGTCAGTTGCAGGTCTCAGTTCTGCGACTCCCTACATGTCATTGGCTCTCAACACAGTCTCAAGACACTTCAGATGTCTTACTGGTTCCATCAAAGATCAGCTCAAGCACATAAGAAAAGCTTTGGGGGAGGATTTCTCATCAGTTAAGATTGCCAGTAATACTGGCGGTAATAGTAGAAGTGATAAAACCATGGGCAAGCTAAAGTATATGGGCCTCGGCTTGCAAAAGCATAAAGCCGCGGCTGGGGTCGATGCCGGCTTCTCTGAACCCCAACAACACGTCTGGAGGCCCCAGAGAGGCCTACCGGATCGTTCAGTGGCCATCCTTAGAGCATGGCTCTTTGATCATTTTCTTCACCCGTATCCAACGGACACAGATAAGCACATGTTAGCCACTCAAACCGGTTTGTCTAGAAACCAGGTGTCAAACTGGTTCATAAATGCCCGAGTGCGTGTATGGAAGCCAATGGTTGAAGAAATACACATGCTTGAGACGCGAGGATCCATGGAAGCTTGCCAAGATCCTGGGAAAGAAGGAAATTCTCCAACCGAAGGCACCAGCAGGCCAAACAATGAGCAACTTGGCATGCATCATGTTATAACTGATAGGCAATTGGAATCCTCTGGTATAGGTTCCTTGAGAGATGCACCGAATGTAGAAGAAGTAGACCAAAGTCAGGAGATCAAGAGGTCCAGAATAGAATGTCAAGTTCCGTCAAGCATGGATGGTGCATTGATGGGTTTTGTGCCATACAATCGAAGCGGGCTTGAGGTTGGGGGACTTGGAGCGGTGTCACTGACATTGGGGCTCAGGCATGGTGTGGAAAACGCCCAACAACAGCAGCAGCAACAATTGCATCAACAGGAGGAATATCAGCTTAGGCGGCAACTTGGAGGTCACATGATTCATGATTTTGTGGGTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0001763 GO:0003006 GO:0003674 GO:0003700 GO:0006355 GO:0007275 GO:0007389 GO:0008150 GO:0009653 GO:0009791 GO:0009888 GO:0009889 GO:0010016 GO:0010073 GO:0010074 GO:0010076 GO:0010077 GO:0010223 GO:0010228 GO:0010229 GO:0010346 GO:0010468 GO:0010556 GO:0019219 GO:0019222 GO:0019827 GO:0022414 GO:0031323 GO:0031326 GO:0032501 GO:0032502 GO:0048367 GO:0048507 GO:0048608 GO:0048646 GO:0048731 GO:0048856 GO:0050789 GO:0050794 GO:0051171 GO:0051252 GO:0060255 GO:0061458 GO:0065007 GO:0080006 GO:0080090 GO:0090567 GO:0098727 GO:0140110 GO:1903506 GO:1905393 GO:2000112 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Homeobox_KN POX

Protein Analysis

762

Amino Acids

83.71

Weight (kDa)

7.17

Isoelectric Point (pI)

53.38

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
POX	PF07526	317 - 485	2e-39	Associated with HOX
Homeobox_KN	PF05920	567 - 606	2.2e-19	Homeobox KN domain
Homeodomain	PF00046	574 - 606	2.9e-06	Homeodomain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0001921)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G27990 AT2G27990
fragaria_vesca	FvH4_3g31640 FvH4_3g31640 FvH4_3g31640 FvH4_3g31640 FvH4_3g31640 FvH4_3g31640 FvH4_3g31640 FvH4_3g31640 FvH4_3g31640 FvH4_3g31640 FvH4_3g31640 FvH4_3g31640 FvH4_3g31640 FvH4_3g31640 FvH4_3g31640 FvH4_3g31640 FvH4_3g31640 FvH4_3g31640 FvH4_3g31640 FvH4_3g31640 FvH4_3g31640 FvH4_3g31640 FvH4_3g31640 FvH4_3g31640
malus_domestica	MD03G1122400.v1.1 MD11G1140600.v1.1
prunus_persica	Prupe.6G105100_v2.0.a1 Prupe.6G105100_v2.0.a1 Prupe.6G105100_v2.0.a1 Prupe.6G105100_v2.0.a1 Prupe.6G105100_v2.0.a1 Prupe.6G105100_v2.0.a1
pyrus_communis	pycom11g11630
rosa_chinensis	RchiOBHm_Chr5g0058351
rosa_laevigata	RLG00000035214
rosa_roxburghii	Rroxscaffold_1G00022230
rosa_rugosa	Rorug05G0313100 Rorug05G0313200 Rorug05G0313300
rosa_samantha	Rh5AG380800 Rh5BG392300 Rh5CG416300 Rh5DG407200
rosa_wichuraiana	Rw5G035880

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	824, 1964
Acc36I	ACCTGC	1 cut(s)	1364
AccB1I	GGYRCC	1 cut(s)	1919
AccBSI	CCGCTC	2 cut(s)	666, 2153
AccI	GTMKAC	1 cut(s)	2031
AccII	CGCG	2 cut(s)	1604, 1861
AclWI	GGATC	8 cut(s)	194, 555, 639, 1071, 1680, 1860, 1873, 1880
AcoI	YGGCCR	1 cut(s)	1683
AcsI	RAATTY	5 cut(s)	433, 998, 1025, 1132, 1903
AcuI	CTGAAG	1 cut(s)	1417
AcyI	GRCGYC	1 cut(s)	1043
AfaI	GTAC	6 cut(s)	120, 370, 448, 772, 1265, 1319
AfiI	CCNNNNNNNGG	5 cut(s)	935, 1608, 1661, 1916, 2041
AflIII	ACRYGT	3 cut(s)	1398, 1642, 1749
AgeI	ACCGGT	1 cut(s)	1766
AgsI	TTSAA	9 cut(s)	77, 161, 378, 738, 857, 955, 973, 1030, 1838
AhlI	ACTAGT	2 cut(s)	872, 887
AjiI	CACGTC	2 cut(s)	1239, 1645
AjnI	CCWGG	3 cut(s)	442, 1782, 1888
Alw26I	GTCTC	6 cut(s)	726, 1105, 1357, 1382, 1426, 1850
AlwI	GGATC	8 cut(s)	194, 555, 639, 1071, 1680, 1860, 1873, 1880
Ama87I	CYCGRG	2 cut(s)	616, 1809
AoxI	GGCC	8 cut(s)	20, 715, 1256, 1576, 1652, 1663, 1683, 1928
ApeKI	GCWGC	7 cut(s)	896, 1115, 1223, 1226, 1229, 2206, 2209
ApoI	RAATTY	5 cut(s)	433, 998, 1025, 1132, 1903
AsiGI	ACCGGT	1 cut(s)	1766
Asp700I	GAANNNNTTC	2 cut(s)	1025, 1162
AspS9I	GGNCC	5 cut(s)	917, 926, 1576, 1653, 2055
AsuHPI	GGTGA	3 cut(s)	123, 293, 1712
AsuII	TTCGAA	3 cut(s)	166, 1019, 1196
AvaI	CYCGRG	2 cut(s)	616, 1809
AvaII	GGWCC	3 cut(s)	917, 926, 2055
BalI	TGGCCA	1 cut(s)	1685
BamHI	GGATCC	1 cut(s)	1865
BanI	GGYRCC	1 cut(s)	1919
BanII	GRGCYC	1 cut(s)	2175
BauI	CACGAG	1 cut(s)	361
BbsI	GAAGAC	1 cut(s)	1233
BbvI	GCAGC	7 cut(s)	883, 1102, 1210, 1213, 1216, 2218, 2221
BceAI	ACGGC	1 cut(s)	42
BciT130I	CCWGG	3 cut(s)	444, 1784, 1890
BciVI	GTATCC	1 cut(s)	1737
BclI	TGATCA	2 cut(s)	1108, 1708
BcoDI	GTCTC	6 cut(s)	726, 1105, 1357, 1382, 1426, 1850
BcuI	ACTAGT	2 cut(s)	872, 887
BfaI	CTAG	7 cut(s)	24, 554, 843, 873, 888, 1011, 1776
BfmI	CTRYAG	2 cut(s)	519, 1003
BfuAI	ACCTGC	1 cut(s)	1364
BfuI	GTATCC	1 cut(s)	1737
BlpI	GCTNAGC	1 cut(s)	45
BmcAI	AGTACT	1 cut(s)	772
Bme1390I	CCNGG	3 cut(s)	444, 1784, 1890
Bme18I	GGWCC	3 cut(s)	917, 926, 2055
BmeT110I	CYCGRG	2 cut(s)	616, 1809
BmgBI	CACGTC	2 cut(s)	1239, 1645
BmgT120I	GGNCC	5 cut(s)	917, 926, 1576, 1653, 2055
BmiI	GGNNCC	6 cut(s)	918, 1450, 1655, 1867, 1921, 1999
BmrFI	CCNGG	3 cut(s)	444, 1784, 1890
BmrI	ACTGGG	1 cut(s)	786
BmsI	GCATC	6 cut(s)	269, 1181, 1606, 1963, 1999, 2230
BmuI	ACTGGG	1 cut(s)	786
BoxI	GACNNNNGTC	2 cut(s)	288, 2037
BpiI	GAAGAC	1 cut(s)	1233
BplI	GAGNNNNNCTC	4 cut(s)	1484, 1516, 1686, 1718
BpmI	CTGGAG	2 cut(s)	682, 1669
Bpu10I	CCTNAGC	2 cut(s)	2174, 2243
Bpu1102I	GCTNAGC	1 cut(s)	45
Bpu14I	TTCGAA	3 cut(s)	166, 1019, 1196
BpuEI	CTTGAG	7 cut(s)	255, 558, 1408, 1451, 1874, 2023, 2153
BsaHI	GRCGYC	1 cut(s)	1043
BsaI	GGTCTC	2 cut(s)	1357, 1382
BsaJI	CCNNGG	6 cut(s)	454, 1554, 1579, 1602, 1869, 1889
BsaWI	WCCGGW	3 cut(s)	530, 1669, 1766
Bsc4I	CCNNNNNNNGG	5 cut(s)	935, 1608, 1661, 1916, 2041
Bse118I	RCCGGY	3 cut(s)	976, 1619, 1766
Bse1I	ACTGG	8 cut(s)	665, 781, 940, 1071, 1450, 1518, 1531, 1799
BseBI	CCWGG	3 cut(s)	444, 1784, 1890
BseDI	CCNNGG	6 cut(s)	454, 1554, 1579, 1602, 1869, 1889
BseGI	GGATG	5 cut(s)	419, 633, 1175, 1686, 2095
BseLI	CCNNNNNNNGG	5 cut(s)	935, 1608, 1661, 1916, 2041
BseMII	CTCAG	6 cut(s)	59, 308, 1038, 1236, 1393, 2188
BseNI	ACTGG	8 cut(s)	665, 781, 940, 1071, 1450, 1518, 1531, 1799
BseRI	GAGGAG	1 cut(s)	1316
BseXI	GCAGC	7 cut(s)	883, 1102, 1210, 1213, 1216, 2218, 2221
BseYI	CCCAGC	1 cut(s)	1607
Bsh1236I	CGCG	2 cut(s)	1604, 1861
BshFI	GGCC	8 cut(s)	22, 717, 1258, 1578, 1654, 1665, 1685, 1930
BshNI	GGYRCC	1 cut(s)	1919
BshTI	ACCGGT	1 cut(s)	1766
BsiHKCI	CYCGRG	2 cut(s)	616, 1809
BsiSI	CCGG	6 cut(s)	531, 977, 1259, 1620, 1670, 1767
BslFI	GGGAC	2 cut(s)	883, 2157
BslI	CCNNNNNNNGG	5 cut(s)	935, 1608, 1661, 1916, 2041
BsmAI	GTCTC	6 cut(s)	726, 1105, 1357, 1382, 1426, 1850
BsmBI	CGTCTC	1 cut(s)	1850
BsmFI	GGGAC	2 cut(s)	883, 2157
BsnI	GGCC	8 cut(s)	22, 717, 1258, 1578, 1654, 1665, 1685, 1930
Bso31I	GGTCTC	2 cut(s)	1357, 1382
BsoBI	CYCGRG	2 cut(s)	616, 1809
Bsp119I	TTCGAA	3 cut(s)	166, 1019, 1196
Bsp1286I	GDGCHC	1 cut(s)	2175
Bsp1720I	GCTNAGC	1 cut(s)	45
Bsp19I	CCATGG	3 cut(s)	454, 1554, 1869
BspANI	GGCC	8 cut(s)	22, 717, 1258, 1578, 1654, 1665, 1685, 1930
BspCNI	CTCAG	6 cut(s)	58, 307, 1039, 1237, 1392, 2187
BspFNI	CGCG	2 cut(s)	1604, 1861
BspHI	TCATGA	1 cut(s)	2272
BspLI	GGNNCC	6 cut(s)	918, 1450, 1655, 1867, 1921, 1999
BspMI	ACCTGC	1 cut(s)	1364
BspPI	GGATC	8 cut(s)	194, 555, 639, 1071, 1680, 1860, 1873, 1880
BspT104I	TTCGAA	3 cut(s)	166, 1019, 1196
BspT107I	GGYRCC	1 cut(s)	1919
BspTNI	GGTCTC	2 cut(s)	1357, 1382
BsrBI	CCGCTC	2 cut(s)	666, 2153
BsrFI	RCCGGY	3 cut(s)	976, 1619, 1766
BsrI	ACTGG	8 cut(s)	665, 781, 940, 1071, 1450, 1518, 1531, 1799
BssAI	RCCGGY	3 cut(s)	976, 1619, 1766
BssECI	CCNNGG	6 cut(s)	454, 1554, 1579, 1602, 1869, 1889
BssNI	GRCGYC	1 cut(s)	1043
BssSI	CACGAG	1 cut(s)	361
BssT1I	CCWWGG	3 cut(s)	454, 1554, 1869
Bst2BI	CACGAG	1 cut(s)	361
Bst2UI	CCWGG	3 cut(s)	444, 1784, 1890
Bst4CI	ACNGT	3 cut(s)	1099, 1193, 1420
Bst6I	CTCTTC	3 cut(s)	215, 950, 1360
BstACI	GRCGYC	1 cut(s)	1043
BstAPI	GCANNNNNTGC	2 cut(s)	251, 2218
BstBI	TTCGAA	3 cut(s)	166, 1019, 1196
BstDEI	CTNAG	9 cut(s)	45, 294, 337, 1047, 1245, 1379, 1691, 2174, 2243
BstDSI	CCRYGG	4 cut(s)	454, 1554, 1602, 1869
BstEII	GGTNACC	1 cut(s)	349
BstF5I	GGATG	5 cut(s)	419, 633, 1175, 1686, 2095
BstFNI	CGCG	2 cut(s)	1604, 1861
BstMAI	GTCTC	6 cut(s)	726, 1105, 1357, 1382, 1426, 1850
BstMWI	GCNNNNNNNGC	7 cut(s)	251, 266, 550, 1229, 1858, 2218, 2248
BstNI	CCWGG	3 cut(s)	444, 1784, 1890
BstNSI	RCATGY	5 cut(s)	1297, 1402, 1753, 1852, 1954
BstPAI	GACNNNNGTC	2 cut(s)	288, 2037
BstPI	GGTNACC	1 cut(s)	349
BstSCI	CCNGG	3 cut(s)	442, 1782, 1888
BstSFI	CTRYAG	2 cut(s)	519, 1003
BstUI	CGCG	2 cut(s)	1604, 1861
BstV1I	GCAGC	7 cut(s)	883, 1102, 1210, 1213, 1216, 2218, 2221
BstV2I	GAAGAC	1 cut(s)	1233
BstX2I	RGATCY	2 cut(s)	1865, 1885
BstXI	CCANNNNNNTGG	2 cut(s)	601, 1889
BstYI	RGATCY	2 cut(s)	1865, 1885
BsuI	GTATCC	1 cut(s)	1737
BsuRI	GGCC	8 cut(s)	22, 717, 1258, 1578, 1654, 1665, 1685, 1930
BtgI	CCRYGG	4 cut(s)	454, 1554, 1602, 1869
BtrI	CACGTC	2 cut(s)	1239, 1645
BtsCI	GGATG	5 cut(s)	419, 633, 1175, 1686, 2095
BtsI	GCAGTG	1 cut(s)	1216
BtsIMutI	CAGTG	5 cut(s)	933, 1078, 1216, 1686, 2159
BveI	ACCTGC	1 cut(s)	1364
CciI	TCATGA	1 cut(s)	2272
Cfr10I	RCCGGY	3 cut(s)	976, 1619, 1766
Cfr13I	GGNCC	5 cut(s)	917, 926, 1576, 1653, 2055
Cfr42I	CCGCGG	1 cut(s)	1605
CseI	GACGC	2 cut(s)	1032, 1867
CsiI	ACCWGGT	1 cut(s)	1782
Csp6I	GTAC	6 cut(s)	119, 369, 447, 771, 1264, 1318
CspAI	ACCGGT	1 cut(s)	1766
CviQI	GTAC	6 cut(s)	119, 369, 447, 771, 1264, 1318
DdeI	CTNAG	9 cut(s)	45, 294, 337, 1047, 1245, 1379, 1691, 2174, 2243
DraI	TTTAAA	1 cut(s)	438
EaeI	YGGCCR	1 cut(s)	1683
Eam1104I	CTCTTC	3 cut(s)	215, 950, 1360
EarI	CTCTTC	3 cut(s)	215, 950, 1360
EciI	GGCGGA	1 cut(s)	468
Eco130I	CCWWGG	3 cut(s)	454, 1554, 1869
Eco147I	AGGCCT	3 cut(s)	22, 717, 1665
Eco24I	GRGCYC	1 cut(s)	2175
Eco31I	GGTCTC	2 cut(s)	1357, 1382
Eco32I	GATATC	1 cut(s)	1056
Eco47I	GGWCC	3 cut(s)	917, 926, 2055
Eco57I	CTGAAG	1 cut(s)	1417
Eco88I	CYCGRG	2 cut(s)	616, 1809
Eco91I	GGTNACC	1 cut(s)	349
EcoO109I	RGGNCCY	2 cut(s)	917, 1653
EcoO65I	GGTNACC	1 cut(s)	349
EcoRI	GAATTC	2 cut(s)	998, 1025
EcoRII	CCWGG	3 cut(s)	442, 1782, 1888
EcoRV	GATATC	1 cut(s)	1056
EcoT14I	CCWWGG	3 cut(s)	454, 1554, 1869
EcoT22I	ATGCAT	3 cut(s)	256, 1295, 1956
EcoT38I	GRGCYC	1 cut(s)	2175
ErhI	CCWWGG	3 cut(s)	454, 1554, 1869
Esp3I	CGTCTC	1 cut(s)	1850
FalI	AAGNNNNNCTT	4 cut(s)	1225, 1257, 1468, 1500
FaqI	GGGAC	2 cut(s)	883, 2157
FauI	CCCGC	2 cut(s)	659, 2120
FauNDI	CATATG	1 cut(s)	387
FbaI	TGATCA	2 cut(s)	1108, 1708
FblI	GTMKAC	1 cut(s)	2031
FokI	GGATG	5 cut(s)	406, 640, 1162, 1673, 2102
FriOI	GRGCYC	1 cut(s)	2175
FspBI	CTAG	7 cut(s)	24, 554, 843, 873, 888, 1011, 1776
GsaI	CCCAGC	1 cut(s)	1611
GsuI	CTGGAG	2 cut(s)	682, 1669
HaeIII	GGCC	8 cut(s)	22, 717, 1258, 1578, 1654, 1665, 1685, 1930
HapII	CCGG	6 cut(s)	531, 977, 1259, 1620, 1670, 1767
HgaI	GACGC	2 cut(s)	1032, 1867
Hin1I	GRCGYC	1 cut(s)	1043
HindIII	AAGCTT	3 cut(s)	334, 1482, 1875
HinfI	GANTC	9 cut(s)	29, 289, 571, 606, 1247, 1364, 1390, 1982, 2269
HpaII	CCGG	6 cut(s)	531, 977, 1259, 1620, 1670, 1767
HphI	GGTGA	3 cut(s)	123, 293, 1712
Hpy166II	GTNNAC	2 cut(s)	33, 2032
Hpy188I	TCNGA	7 cut(s)	176, 204, 297, 1048, 1246, 1436, 1630
Hpy8I	GTNNAC	2 cut(s)	33, 2032
HpyAV	CCTTC	4 cut(s)	759, 1270, 1892, 1910
HpyCH4III	ACNGT	3 cut(s)	1099, 1193, 1420
HpyCH4IV	ACGT	3 cut(s)	1034, 1238, 1644
HpyF10VI	GCNNNNNNNGC	7 cut(s)	251, 266, 550, 1229, 1858, 2218, 2248
HpyF3I	CTNAG	9 cut(s)	45, 294, 337, 1047, 1245, 1379, 1691, 2174, 2243
HpySE526I	ACGT	3 cut(s)	1034, 1238, 1644
Hsp92I	GRCGYC	1 cut(s)	1043
KroI	GCCGGC	1 cut(s)	1619
KroNI	GCCGGC	1 cut(s)	1621
Ksp22I	TGATCA	2 cut(s)	1108, 1708
KspI	CCGCGG	1 cut(s)	1605
LmnI	GCTCC	4 cut(s)	663, 1220, 1290, 2150
Lsp1109I	GCAGC	7 cut(s)	883, 1102, 1210, 1213, 1216, 2218, 2221
LweI	GCATC	6 cut(s)	269, 1181, 1606, 1963, 1999, 2230
MabI	ACCWGGT	1 cut(s)	1782
MaeI	CTAG	7 cut(s)	24, 554, 843, 873, 888, 1011, 1776
MaeII	ACGT	3 cut(s)	1034, 1238, 1644
MaeIII	GTNAC	8 cut(s)	281, 349, 407, 514, 791, 1093, 2157, 2261
MbiI	CCGCTC	2 cut(s)	666, 2153
MfeI	CAATTG	4 cut(s)	639, 758, 1976, 2216
MflI	RGATCY	2 cut(s)	1865, 1885
MhlI	GDGCHC	1 cut(s)	2175
MlsI	TGGCCA	1 cut(s)	1685
MluNI	TGGCCA	1 cut(s)	1685
MlyI	GAGTC	6 cut(s)	38, 298, 565, 1256, 1373, 1384
MmeI	TCCRAC	4 cut(s)	227, 972, 1754, 1934
Mox20I	TGGCCA	1 cut(s)	1685
Mph1103I	ATGCAT	3 cut(s)	256, 1295, 1956
MroNI	GCCGGC	1 cut(s)	1619
MroXI	GAANNNNTTC	2 cut(s)	1025, 1162
MscI	TGGCCA	1 cut(s)	1685
MseI	TTAA	3 cut(s)	437, 1509, 2287
Msp20I	TGGCCA	1 cut(s)	1685
MspA1I	CMGCKG	2 cut(s)	988, 1604
MspI	CCGG	6 cut(s)	531, 977, 1259, 1620, 1670, 1767
MspR9I	CCNGG	3 cut(s)	444, 1784, 1890
MunI	CAATTG	4 cut(s)	639, 758, 1976, 2216
MvaI	CCWGG	3 cut(s)	444, 1784, 1890
MvnI	CGCG	2 cut(s)	1604, 1861
MwoI	GCNNNNNNNGC	7 cut(s)	251, 266, 550, 1229, 1858, 2218, 2248
NaeI	GCCGGC	1 cut(s)	1621
NcoI	CCATGG	3 cut(s)	454, 1554, 1869
NdeI	CATATG	1 cut(s)	387
NgoMIV	GCCGGC	1 cut(s)	1619
NlaIV	GGNNCC	6 cut(s)	918, 1450, 1655, 1867, 1921, 1999
NmeAIII	GCCGAG	1 cut(s)	1560
NmuCI	GTSAC	4 cut(s)	281, 514, 2157, 2261
NsiI	ATGCAT	3 cut(s)	256, 1295, 1956
NspI	RCATGY	5 cut(s)	1297, 1402, 1753, 1852, 1954
NspV	TTCGAA	3 cut(s)	166, 1019, 1196
PaeI	GCATGC	2 cut(s)	1297, 1954
PaeR7I	CTCGAG	1 cut(s)	616
PagI	TCATGA	1 cut(s)	2272
PceI	AGGCCT	3 cut(s)	22, 717, 1665
PciI	ACATGT	2 cut(s)	1398, 1749
PcsI	WCGNNNNNNNCGW	1 cut(s)	1040
PdiI	GCCGGC	1 cut(s)	1621
PdmI	GAANNNNTTC	2 cut(s)	1025, 1162
PfeI	GAWTC	3 cut(s)	606, 1982, 2269
PinAI	ACCGGT	1 cut(s)	1766
PleI	GAGTC	6 cut(s)	37, 297, 565, 1255, 1372, 1384
PpsI	GAGTC	6 cut(s)	37, 297, 565, 1255, 1372, 1384
PpuMI	RGGWCCY	1 cut(s)	917
PscI	ACATGT	2 cut(s)	1398, 1749
PshAI	GACNNNNGTC	2 cut(s)	288, 2037
PsiI	TTATAA	2 cut(s)	824, 1964
Psp5II	RGGWCCY	1 cut(s)	917
Psp6I	CCWGG	3 cut(s)	442, 1782, 1888
PspEI	GGTNACC	1 cut(s)	349
PspFI	CCCAGC	1 cut(s)	1607
PspGI	CCWGG	3 cut(s)	442, 1782, 1888
PspN4I	GGNNCC	6 cut(s)	918, 1450, 1655, 1867, 1921, 1999
PspPI	GGNCC	5 cut(s)	917, 926, 1576, 1653, 2055
PspPPI	RGGWCCY	1 cut(s)	917
PspXI	VCTCGAGB	1 cut(s)	616
PsuI	RGATCY	2 cut(s)	1865, 1885
PvuII	CAGCTG	1 cut(s)	988
RsaI	GTAC	6 cut(s)	120, 370, 448, 772, 1265, 1319
RsaNI	GTAC	6 cut(s)	119, 369, 447, 771, 1264, 1318
SacII	CCGCGG	1 cut(s)	1605
SaqAI	TTAA	3 cut(s)	437, 1509, 2287
Sau96I	GGNCC	5 cut(s)	917, 926, 1576, 1653, 2055
ScaI	AGTACT	1 cut(s)	772
SchI	GAGTC	6 cut(s)	38, 298, 565, 1256, 1373, 1384
ScrFI	CCNGG	3 cut(s)	444, 1784, 1890
SduI	GDGCHC	1 cut(s)	2175
SexAI	ACCWGGT	1 cut(s)	1782
SfaNI	GCATC	6 cut(s)	269, 1181, 1606, 1963, 1999, 2230
SfcI	CTRYAG	2 cut(s)	519, 1003
Sfr274I	CTCGAG	1 cut(s)	616
Sfr303I	CCGCGG	1 cut(s)	1605
SfuI	TTCGAA	3 cut(s)	166, 1019, 1196
SgrBI	CCGCGG	1 cut(s)	1605
SinI	GGWCC	3 cut(s)	917, 926, 2055
SlaI	CTCGAG	1 cut(s)	616
SmlI	CTYRAG	8 cut(s)	270, 573, 616, 1423, 1466, 1853, 2002, 2132
SmoI	CTYRAG	8 cut(s)	270, 573, 616, 1423, 1466, 1853, 2002, 2132
SpeI	ACTAGT	2 cut(s)	872, 887
SphI	GCATGC	2 cut(s)	1297, 1954
SseBI	AGGCCT	3 cut(s)	22, 717, 1665
SspMI	CTAG	7 cut(s)	24, 554, 843, 873, 888, 1011, 1776
StuI	AGGCCT	3 cut(s)	22, 717, 1665
StyD4I	CCNGG	3 cut(s)	442, 1782, 1888
StyI	CCWWGG	3 cut(s)	454, 1554, 1869
TaaI	ACNGT	3 cut(s)	1099, 1193, 1420
TaiI	ACGT	3 cut(s)	1037, 1241, 1647
TatI	WGTACW	2 cut(s)	770, 1317
TauI	GCSGC	4 cut(s)	57, 1604, 1607, 2251
TfiI	GAWTC	3 cut(s)	606, 1982, 2269
Tru1I	TTAA	3 cut(s)	437, 1509, 2287
Tru9I	TTAA	3 cut(s)	437, 1509, 2287
TscAI	CASTG	5 cut(s)	940, 1078, 1216, 1686, 2166
TseFI	GTSAC	4 cut(s)	281, 514, 2157, 2261
TseI	GCWGC	7 cut(s)	896, 1115, 1223, 1226, 1229, 2206, 2209
Tsp45I	GTSAC	4 cut(s)	281, 514, 2157, 2261
TspDTI	ATGAA	5 cut(s)	555, 824, 1215, 1789, 2261
TspGWI	ACGGA	4 cut(s)	1081, 1126, 1748, 2067
TspRI	CASTG	5 cut(s)	940, 1078, 1216, 1686, 2166
VpaK11BI	GGWCC	3 cut(s)	917, 926, 2055
XapI	RAATTY	5 cut(s)	433, 998, 1025, 1132, 1903
XbaI	TCTAGA	1 cut(s)	1775
XceI	RCATGY	5 cut(s)	1297, 1402, 1753, 1852, 1954
XhoI	CTCGAG	1 cut(s)	616
XmiI	GTMKAC	1 cut(s)	2031
XmnI	GAANNNNTTC	2 cut(s)	1025, 1162
XspI	CTAG	7 cut(s)	24, 554, 843, 873, 888, 1011, 1776
ZrmI	AGTACT	1 cut(s)	772
Zsp2I	ATGCAT	3 cut(s)	256, 1295, 1956

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.