MD03G1125100.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0007829
Physical Location & Seq
Reverse (-)
11795963 .. 11800649
4687 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1125100.v1.1.491

Sequence Viewer

Length: 441 bp
ATGGAGTTGGCTGTGCGCCAAATATCATTATCTTCCCTTCCTAAGATCAATGTAAAGCCACGAGAGCAAAGAAGAAGACAATCATCATTGGTATCTTCTGCCCTACCTGAAACAGTGGCTTCAATAGCGATTGCTGCTACAGTCGTTGGTACAGCAGCTGCGGTTCTTGCAAAGAGAACCAAAGCTGCTGAAGAAGCTGAGGTTCCTATGAAAATATGTGAAGCCTGTCGTGGTTCTGGTATATGTCCTGAATGCAAAGGCGAAGGCTTTGTACTCAAGAAACTCTCTGACGAAAGTGCTGAGAGGGCAAGAATGGCTTCTAAGAATATGGCCACTCGATACACAGCAGGGCTTCCAAAGAAATGGAGCTATTGCACAAAATGCTCTTCTGCGCGATCATGCCTTGCCTGCAGTGGCCGTGGAAAGATAGCTTTGCTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

147

Amino Acids

15.55

Weight (kDa)

9.73

Isoelectric Point (pI)

53.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7895 PF25433 70 - 143 2.7e-40 Domain of unknown function (DUF7895)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 394
AciI CCGC 1 cut(s) 161
AcoI YGGCCR 2 cut(s) 330, 415
AcuI CTGAAG 1 cut(s) 210
AfaI GTAC 2 cut(s) 151, 273
AgsI TTSAA 1 cut(s) 123
AluBI AGCT 5 cut(s) 158, 185, 197, 369, 431
AluI AGCT 5 cut(s) 158, 185, 197, 369, 431
AlwNI CAGNNNCTG 1 cut(s) 158
AoxI GGCC 2 cut(s) 330, 415
ApeKI GCWGC 4 cut(s) 134, 155, 158, 185
Asp700I GAANNNNTTC 1 cut(s) 316
AspLEI GCGC 2 cut(s) 18, 394
BalI TGGCCA 1 cut(s) 332
BarI GAAGNNNNNNTAC 2 cut(s) 255, 287
BauI CACGAG 1 cut(s) 60
BbsI GAAGAC 1 cut(s) 82
BbvCI CCTCAGC 1 cut(s) 198
BbvI GCAGC 4 cut(s) 121, 145, 167, 172
BceAI ACGGC 1 cut(s) 402
BfmI CTRYAG 2 cut(s) 138, 409
BisI GCNGC 4 cut(s) 135, 156, 159, 186
BlsI GCNGC 4 cut(s) 136, 157, 160, 187
BmiI GGNNCC 1 cut(s) 204
BpiI GAAGAC 1 cut(s) 82
Bpu10I CCTNAGC 1 cut(s) 198
BpuEI CTTGAG 1 cut(s) 260
BsaJI CCNNGG 1 cut(s) 418
BseDI CCNNGG 1 cut(s) 418
BseMII CTCAG 2 cut(s) 189, 291
BseXI GCAGC 4 cut(s) 121, 145, 167, 172
Bsh1236I CGCG 1 cut(s) 394
BshFI GGCC 2 cut(s) 332, 417
BsmI GAATGC 1 cut(s) 257
BsnI GGCC 2 cut(s) 332, 417
Bsp143I GATC 2 cut(s) 45, 395
BspACI CCGC 1 cut(s) 161
BspANI GGCC 2 cut(s) 332, 417
BspCNI CTCAG 2 cut(s) 190, 292
BspFNI CGCG 1 cut(s) 394
BspLI GGNNCC 1 cut(s) 204
BspMAI CTGCAG 1 cut(s) 413
BspQI GCTCTTC 1 cut(s) 391
BssECI CCNNGG 1 cut(s) 418
BssMI GATC 2 cut(s) 45, 395
BssSI CACGAG 1 cut(s) 60
Bst2BI CACGAG 1 cut(s) 60
Bst4CI ACNGT 2 cut(s) 115, 142
Bst6I CTCTTC 1 cut(s) 391
BstAPI GCANNNNNTGC 1 cut(s) 381
BstC8I GCNNGC 1 cut(s) 409
BstDEI CTNAG 4 cut(s) 42, 198, 300, 321
BstDSI CCRYGG 1 cut(s) 418
BstFNI CGCG 1 cut(s) 394
BstHHI GCGC 2 cut(s) 18, 394
BstKTI GATC 2 cut(s) 48, 398
BstMBI GATC 2 cut(s) 45, 395
BstMWI GCNNNNNNNGC 9 cut(s) 64, 125, 134, 167, 194, 305, 314, 381, 408
BstSFI CTRYAG 2 cut(s) 138, 409
BstUI CGCG 1 cut(s) 394
BstV1I GCAGC 4 cut(s) 121, 145, 167, 172
BstV2I GAAGAC 1 cut(s) 82
BstXI CCANNNNNNTGG 1 cut(s) 363
BsuRI GGCC 2 cut(s) 332, 417
BtgI CCRYGG 1 cut(s) 418
BtsI GCAGTG 1 cut(s) 418
BtsIMutI CAGTG 2 cut(s) 120, 418
Cac8I GCNNGC 1 cut(s) 409
CaiI CAGNNNCTG 1 cut(s) 158
CfoI GCGC 2 cut(s) 18, 394
Csp6I GTAC 2 cut(s) 150, 272
CviAII CATG 1 cut(s) 399
CviQI GTAC 2 cut(s) 150, 272
DdeI CTNAG 4 cut(s) 42, 198, 300, 321
DpnI GATC 2 cut(s) 47, 397
DpnII GATC 2 cut(s) 45, 395
EaeI YGGCCR 2 cut(s) 330, 415
Eam1104I CTCTTC 1 cut(s) 391
EarI CTCTTC 1 cut(s) 391
Eco57I CTGAAG 1 cut(s) 210
FaeI CATG 1 cut(s) 402
FaiI YATR 6 cut(s) 209, 217, 242, 244, 329, 400
FalI AAGNNNNNCTT 2 cut(s) 301, 333
FatI CATG 1 cut(s) 398
Fnu4HI GCNGC 4 cut(s) 135, 156, 159, 186
Fsp4HI GCNGC 4 cut(s) 135, 156, 159, 186
GlaI GCGC 2 cut(s) 17, 393
GluI GCNGC 4 cut(s) 135, 156, 159, 186
HaeIII GGCC 2 cut(s) 332, 417
HhaI GCGC 2 cut(s) 18, 394
Hin1II CATG 1 cut(s) 402
Hin6I GCGC 2 cut(s) 16, 392
HinP1I GCGC 2 cut(s) 16, 392
Hpy188I TCNGA 1 cut(s) 289
Hpy188III TCNNGA 2 cut(s) 248, 277
HpyAV CCTTC 2 cut(s) 47, 257
HpyCH4III ACNGT 2 cut(s) 115, 142
HpyCH4V TGCA 4 cut(s) 170, 255, 375, 411
HpyF10VI GCNNNNNNNGC 9 cut(s) 64, 125, 134, 167, 194, 305, 314, 381, 408
HpyF3I CTNAG 4 cut(s) 42, 198, 300, 321
Hsp92II CATG 1 cut(s) 402
HspAI GCGC 2 cut(s) 16, 392
Kzo9I GATC 2 cut(s) 45, 395
LguI GCTCTTC 1 cut(s) 391
LmnI GCTCC 1 cut(s) 366
LpnPI CCDG 6 cut(s) 120, 222, 238, 261, 333, 421
Lsp1109I GCAGC 4 cut(s) 121, 145, 167, 172
MalI GATC 2 cut(s) 47, 397
MboI GATC 2 cut(s) 45, 395
MboII GAAGA 6 cut(s) 24, 84, 87, 87, 203, 378
MlsI TGGCCA 1 cut(s) 332
MluNI TGGCCA 1 cut(s) 332
MnlI CCTC 2 cut(s) 193, 297
Mox20I TGGCCA 1 cut(s) 332
MroXI GAANNNNTTC 1 cut(s) 316
MscI TGGCCA 1 cut(s) 332
MseI TTAA 1 cut(s) 439
Msp20I TGGCCA 1 cut(s) 332
MspA1I CMGCKG 1 cut(s) 158
Mva1269I GAATGC 1 cut(s) 257
MvnI CGCG 1 cut(s) 394
MwoI GCNNNNNNNGC 9 cut(s) 64, 125, 134, 167, 194, 305, 314, 381, 408
NdeII GATC 2 cut(s) 45, 395
NlaIII CATG 1 cut(s) 402
NlaIV GGNNCC 1 cut(s) 204
PciSI GCTCTTC 1 cut(s) 391
PctI GAATGC 1 cut(s) 257
PdmI GAANNNNTTC 1 cut(s) 316
PkrI GCNGC 4 cut(s) 136, 157, 160, 187
PspN4I GGNNCC 1 cut(s) 204
PstI CTGCAG 1 cut(s) 413
PstNI CAGNNNCTG 1 cut(s) 158
PvuII CAGCTG 1 cut(s) 158
RsaI GTAC 2 cut(s) 151, 273
RsaNI GTAC 2 cut(s) 150, 272
SapI GCTCTTC 1 cut(s) 391
SaqAI TTAA 1 cut(s) 439
SatI GCNGC 4 cut(s) 135, 156, 159, 186
Sau3AI GATC 2 cut(s) 45, 395
SetI ASST 7 cut(s) 109, 160, 187, 199, 204, 371, 433
SfcI CTRYAG 2 cut(s) 138, 409
SmlI CTYRAG 1 cut(s) 275
SmoI CTYRAG 1 cut(s) 275
SsiI CCGC 1 cut(s) 161
TaaI ACNGT 2 cut(s) 115, 142
TaqI TCGA 1 cut(s) 337
TatI WGTACW 1 cut(s) 271
Tru1I TTAA 1 cut(s) 439
Tru9I TTAA 1 cut(s) 439
TscAI CASTG 2 cut(s) 120, 418
TseI GCWGC 4 cut(s) 134, 155, 158, 185
TspDTI ATGAA 1 cut(s) 224
TspRI CASTG 2 cut(s) 120, 418
XmnI GAANNNNTTC 1 cut(s) 316
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.