MD11G1142300.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0007829
Physical Location & Seq
Reverse (-)
13276574 .. 13279732
3159 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1142300.v1.1.491

Sequence Viewer

Length: 438 bp
ATGGAGCTGGCTGTGCGCAAAATATCATTTTCTTCCCTTCCTAAGATCAACGTAAAGCCGCAAGAGCAAAGAAGACCATCATCCTTGGTATCTTCTGCTCTACCTGAAACAGTGGCTTCAATAGCAATTGCTGCTGCAGTTGTTGGTACAGCAGCTACGATTCTTGCGAAGAGAACCAAAGCTTCTGAGGAAGCAGAGGTTCCTATGAAAATATGCGAAGCCTGTCGTGGGTCTGGGATATGTCCTGAATGCAAAGGTGAAGGCTTTGTACTCAAGAAACTCTCAGACGAAAGTGCTGAGAGGGCAAGATTGGCTTCTAAGAATATGGCCACTCGATACACAGCAGGGCTTCCAAAGAAATGGAGCTACTGCACAAAGTGCTCTTCTGCGCGATCATGCCTTGCCTGCAATGGCCGTGGAAAGATAGTTTTGCTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

146

Amino Acids

15.43

Weight (kDa)

9.6

Isoelectric Point (pI)

50.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7895 PF25433 69 - 142 2.4e-40 Domain of unknown function (DUF7895)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 17
AccII CGCG 1 cut(s) 391
AciI CCGC 1 cut(s) 59
AcoI YGGCCR 2 cut(s) 327, 412
AdeI CACNNNGTG 1 cut(s) 378
AfaI GTAC 2 cut(s) 148, 270
AfiI CCNNNNNNNGG 1 cut(s) 228
AgsI TTSAA 1 cut(s) 120
AluBI AGCT 4 cut(s) 7, 155, 182, 366
AluI AGCT 4 cut(s) 7, 155, 182, 366
Alw21I GWGCWC 1 cut(s) 383
AoxI GGCC 2 cut(s) 327, 412
ApeKI GCWGC 3 cut(s) 131, 134, 152
AspLEI GCGC 2 cut(s) 18, 391
AsuHPI GGTGA 1 cut(s) 269
BalI TGGCCA 1 cut(s) 329
BarI GAAGNNNNNNTAC 2 cut(s) 252, 284
BbsI GAAGAC 1 cut(s) 79
Bbv12I GWGCWC 1 cut(s) 383
BbvI GCAGC 3 cut(s) 118, 121, 164
BccI CCATC 1 cut(s) 85
BceAI ACGGC 1 cut(s) 399
BfmI CTRYAG 1 cut(s) 135
BisI GCNGC 4 cut(s) 59, 132, 135, 153
BlsI GCNGC 4 cut(s) 60, 133, 136, 154
BmiI GGNNCC 1 cut(s) 201
BpiI GAAGAC 1 cut(s) 79
BpuEI CTTGAG 1 cut(s) 257
BsaJI CCNNGG 2 cut(s) 84, 415
Bsc4I CCNNNNNNNGG 1 cut(s) 228
Bse3DI GCAATG 1 cut(s) 415
BseDI CCNNGG 2 cut(s) 84, 415
BseGI GGATG 1 cut(s) 80
BseLI CCNNNNNNNGG 1 cut(s) 228
BseMI GCAATG 1 cut(s) 415
BseMII CTCAG 3 cut(s) 177, 288, 297
BseXI GCAGC 3 cut(s) 118, 121, 164
BsgI GTGCAG 1 cut(s) 355
Bsh1236I CGCG 1 cut(s) 391
BshFI GGCC 2 cut(s) 329, 414
BsiHKAI GWGCWC 1 cut(s) 383
BslI CCNNNNNNNGG 1 cut(s) 228
BsmI GAATGC 1 cut(s) 254
BsnI GGCC 2 cut(s) 329, 414
Bsp1286I GDGCHC 1 cut(s) 383
Bsp143I GATC 2 cut(s) 45, 392
BspACI CCGC 1 cut(s) 59
BspANI GGCC 2 cut(s) 329, 414
BspCNI CTCAG 3 cut(s) 178, 289, 296
BspFNI CGCG 1 cut(s) 391
BspLI GGNNCC 1 cut(s) 201
BspMAI CTGCAG 1 cut(s) 139
BspQI GCTCTTC 1 cut(s) 388
BsrDI GCAATG 1 cut(s) 415
BssECI CCNNGG 2 cut(s) 84, 415
BssMI GATC 2 cut(s) 45, 392
BssT1I CCWWGG 1 cut(s) 84
Bst4CI ACNGT 1 cut(s) 112
Bst6I CTCTTC 2 cut(s) 164, 388
BstAPI GCANNNNNTGC 2 cut(s) 131, 378
BstC8I GCNNGC 2 cut(s) 9, 406
BstDEI CTNAG 5 cut(s) 42, 186, 283, 297, 318
BstDSI CCRYGG 1 cut(s) 415
BstF5I GGATG 1 cut(s) 80
BstFNI CGCG 1 cut(s) 391
BstHHI GCGC 2 cut(s) 18, 391
BstKTI GATC 2 cut(s) 48, 395
BstMBI GATC 2 cut(s) 45, 392
BstMWI GCNNNNNNNGC 8 cut(s) 13, 64, 122, 131, 302, 311, 378, 405
BstSFI CTRYAG 1 cut(s) 135
BstUI CGCG 1 cut(s) 391
BstV1I GCAGC 3 cut(s) 118, 121, 164
BstV2I GAAGAC 1 cut(s) 79
BstXI CCANNNNNNTGG 1 cut(s) 360
BsuRI GGCC 2 cut(s) 329, 414
BtgI CCRYGG 1 cut(s) 415
BtsCI GGATG 1 cut(s) 80
BtsIMutI CAGTG 1 cut(s) 117
Cac8I GCNNGC 2 cut(s) 9, 406
CfoI GCGC 2 cut(s) 18, 391
Csp6I GTAC 2 cut(s) 147, 269
CspCI CAANNNNNGTGG 2 cut(s) 397, 432
CviAII CATG 1 cut(s) 396
CviQI GTAC 2 cut(s) 147, 269
DdeI CTNAG 5 cut(s) 42, 186, 283, 297, 318
DpnI GATC 2 cut(s) 47, 394
DpnII GATC 2 cut(s) 45, 392
DraIII CACNNNGTG 1 cut(s) 378
EaeI YGGCCR 2 cut(s) 327, 412
Eam1104I CTCTTC 2 cut(s) 164, 388
EarI CTCTTC 2 cut(s) 164, 388
Eco130I CCWWGG 1 cut(s) 84
EcoT14I CCWWGG 1 cut(s) 84
ErhI CCWWGG 1 cut(s) 84
FaeI CATG 1 cut(s) 399
FaiI YATR 5 cut(s) 206, 214, 241, 326, 397
FalI AAGNNNNNCTT 2 cut(s) 298, 330
FatI CATG 1 cut(s) 395
Fnu4HI GCNGC 4 cut(s) 59, 132, 135, 153
FokI GGATG 1 cut(s) 67
Fsp4HI GCNGC 4 cut(s) 59, 132, 135, 153
FspI TGCGCA 1 cut(s) 17
GlaI GCGC 2 cut(s) 17, 390
GluI GCNGC 4 cut(s) 59, 132, 135, 153
HaeIII GGCC 2 cut(s) 329, 414
HhaI GCGC 2 cut(s) 18, 391
Hin1II CATG 1 cut(s) 399
Hin6I GCGC 2 cut(s) 16, 389
HinP1I GCGC 2 cut(s) 16, 389
HindIII AAGCTT 1 cut(s) 180
HinfI GANTC 1 cut(s) 160
HphI GGTGA 1 cut(s) 269
Hpy188I TCNGA 2 cut(s) 187, 286
Hpy188III TCNNGA 2 cut(s) 245, 274
HpyAV CCTTC 2 cut(s) 47, 254
HpyCH4III ACNGT 1 cut(s) 112
HpyCH4IV ACGT 1 cut(s) 51
HpyCH4V TGCA 4 cut(s) 137, 252, 372, 408
HpyF10VI GCNNNNNNNGC 8 cut(s) 13, 64, 122, 131, 302, 311, 378, 405
HpyF3I CTNAG 5 cut(s) 42, 186, 283, 297, 318
HpySE526I ACGT 1 cut(s) 51
Hsp92II CATG 1 cut(s) 399
HspAI GCGC 2 cut(s) 16, 389
Kzo9I GATC 2 cut(s) 45, 392
LguI GCTCTTC 1 cut(s) 388
LmnI GCTCC 2 cut(s) 4, 363
LpnPI CCDG 6 cut(s) 117, 219, 235, 258, 330, 418
Lsp1109I GCAGC 3 cut(s) 118, 121, 164
MaeII ACGT 1 cut(s) 51
MalI GATC 2 cut(s) 47, 394
MboI GATC 2 cut(s) 45, 392
MboII GAAGA 5 cut(s) 24, 84, 84, 181, 375
MfeI CAATTG 1 cut(s) 126
MhlI GDGCHC 1 cut(s) 383
MlsI TGGCCA 1 cut(s) 329
MluCI AATT 1 cut(s) 126
MluNI TGGCCA 1 cut(s) 329
MnlI CCTC 3 cut(s) 181, 190, 294
Mox20I TGGCCA 1 cut(s) 329
MscI TGGCCA 1 cut(s) 329
Msp20I TGGCCA 1 cut(s) 329
MunI CAATTG 1 cut(s) 126
Mva1269I GAATGC 1 cut(s) 254
MvnI CGCG 1 cut(s) 391
MwoI GCNNNNNNNGC 8 cut(s) 13, 64, 122, 131, 302, 311, 378, 405
NdeII GATC 2 cut(s) 45, 392
NlaIII CATG 1 cut(s) 399
NlaIV GGNNCC 1 cut(s) 201
NsbI TGCGCA 1 cut(s) 17
PciSI GCTCTTC 1 cut(s) 388
PcsI WCGNNNNNNNCGW 1 cut(s) 164
PctI GAATGC 1 cut(s) 254
PfeI GAWTC 1 cut(s) 160
PkrI GCNGC 4 cut(s) 60, 133, 136, 154
PspN4I GGNNCC 1 cut(s) 201
PstI CTGCAG 1 cut(s) 139
RsaI GTAC 2 cut(s) 148, 270
RsaNI GTAC 2 cut(s) 147, 269
SapI GCTCTTC 1 cut(s) 388
SatI GCNGC 4 cut(s) 59, 132, 135, 153
Sau3AI GATC 2 cut(s) 45, 392
SduI GDGCHC 1 cut(s) 383
SetI ASST 8 cut(s) 9, 54, 106, 157, 184, 201, 259, 368
SfcI CTRYAG 1 cut(s) 135
SmlI CTYRAG 1 cut(s) 272
SmoI CTYRAG 1 cut(s) 272
Sse9I AATT 1 cut(s) 126
SsiI CCGC 1 cut(s) 59
StyI CCWWGG 1 cut(s) 84
TaaI ACNGT 1 cut(s) 112
TaiI ACGT 1 cut(s) 54
TaqI TCGA 1 cut(s) 334
TasI AATT 1 cut(s) 126
TatI WGTACW 1 cut(s) 268
TauI GCSGC 1 cut(s) 61
TfiI GAWTC 1 cut(s) 160
TscAI CASTG 1 cut(s) 117
TseI GCWGC 3 cut(s) 131, 134, 152
TspDTI ATGAA 1 cut(s) 221
TspRI CASTG 1 cut(s) 117
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.