MD03G1135700.v1.1

Plant protein of unknown function (DUF868)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0012206
Physical Location & Seq
Forward (+)
13588608 .. 13589513
906 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1135700.v1.1.491

Sequence Viewer

Length: 906 bp
ATGAGAGACAAAGTTGCTTGTTTCAATGAAAATGCAGTAAATGTGTCTCATCCTTCATGTTCTAGCTATGCAAACACTTCTTGTGTTTCTCCAAACTTAACTCCTTCAGTCCAAAATGCAGTCTCATGCTTCTACAAAATCACTCTCTCCTCCACTCAAAAGCAGCTTTTGGTCACAGTCTCCTGGTGCAAAAACCACTACGCACAAGGCTTGACCATTAAGCTTTCTGACGATCCCTCAGCAGCATCCTTCAAGTTGAACACAAATTCGGGTATCTTCAGAAAGAAGAAAGGCACCAAAGTCATCGAATCTGATAATTTGAAGACAGAAATCTTTTGGGATCTCTCTAGAGCAAAGTATGATGTTAGTGGTCCTGAACCTGTTGAGGGTTTTTACATTTTGGTCATGATTGATTCAGAAATAGGCCTAGTTCTTGGTGACATGGCCGGAGAAGCTGTAAGCAAGAAGTTCAAGACCAGCGGTGTTGCGAAAGCCTCTCTTGTTTCGAGGCAAGAGCATTGTTCCGGAAATGCTCTTTATTCCACCCGAGCTCAATTCAGTGACACAGGCATTGAACATGACATTTTGATAAAATATAGTGGGGAAAATGAAGGCTTAAAACATCCGGTTTTATCCGTTTGCATCGATAAAAAGACGGTCATTCGAGTAAAGAGGCTGAAGTGGAATTTTAGAGGGAATCAGACAATTTTTGTTGATGGGTTGCTTGTTGATCTGATGTGGGATGTTCATAACTGGTTCTTCAATCCTTCATCAAGGTATGCAGTGTTCATGTTCAGAACAAGACGTGGGACGGATAGCAGATTGTGGTTGGAAGAGAAGATGGTGCACAAGGAGCACGACAAAGTTGAATTCTCCTTGTTGATGTATGCCTGTAAGATCTCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF868

Protein Analysis

302

Amino Acids

33.94

Weight (kDa)

8.98

Isoelectric Point (pI)

40.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF868 PF05910 37 - 299 1.8e-88 Plant protein of unknown function (DUF868)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012206)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G27770
fragaria_vesca FvH4_3g24180
malus_domestica MD03G1135700.v1.1 MD11G1158200.v1.1
prunus_persica Prupe.6G120000_v2.0.a1 Prupe.6G120000_v2.0.a1 Prupe.6G120000_v2.0.a1
pyrus_communis pycom03g09160 pycom11g12850
rosa_chinensis RchiOBHm_Chr5g0043291
rosa_laevigata RLG00000034177
rosa_multiflora Rmu_sc0005936.1_g000006
rosa_roxburghii Rroxscaffold_1G00037530
rosa_rugosa Rorug05G0205100
rosa_samantha Rh5BG297100 Rh5CG326900 Rh5DG306700
rosa_wichuraiana Rw5G026880 Rw5G026950

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 293
AccIII TCCGGA 1 cut(s) 524
AciI CCGC 1 cut(s) 480
AclWI GGATC 2 cut(s) 227, 348
AcoI YGGCCR 1 cut(s) 444
AcsI RAATTY 3 cut(s) 265, 685, 869
AcuI CTGAAG 3 cut(s) 90, 262, 698
AfiI CCNNNNNNNGG 1 cut(s) 386
AgsI TTSAA 8 cut(s) 25, 253, 259, 322, 472, 575, 763, 869
AjiI CACGTC 1 cut(s) 806
AjnI CCWGG 1 cut(s) 182
AluBI AGCT 5 cut(s) 66, 166, 223, 455, 551
AluI AGCT 5 cut(s) 66, 166, 223, 455, 551
Alw21I GWGCWC 3 cut(s) 553, 849, 858
Alw26I GTCTC 3 cut(s) 51, 127, 184
Alw44I GTGCAC 1 cut(s) 845
AlwI GGATC 2 cut(s) 227, 348
Ama87I CYCGRG 1 cut(s) 546
Aor13HI TCCGGA 1 cut(s) 524
AoxI GGCC 2 cut(s) 424, 444
ApaLI GTGCAC 1 cut(s) 845
ApeKI GCWGC 2 cut(s) 163, 242
ApoI RAATTY 3 cut(s) 265, 685, 869
AspS9I GGNCC 1 cut(s) 371
AsuHPI GGTGA 1 cut(s) 449
AvaI CYCGRG 1 cut(s) 546
AvaII GGWCC 1 cut(s) 371
BaeGI GKGCMC 1 cut(s) 849
BanI GGYRCC 1 cut(s) 293
BanII GRGCYC 1 cut(s) 553
BbsI GAAGAC 1 cut(s) 329
Bbv12I GWGCWC 3 cut(s) 553, 849, 858
BbvCI CCTCAGC 1 cut(s) 238
BbvI GCAGC 2 cut(s) 175, 254
BccI CCATC 2 cut(s) 710, 835
BciT130I CCWGG 1 cut(s) 184
BcoDI GTCTC 3 cut(s) 51, 127, 184
BfaI CTAG 3 cut(s) 63, 348, 428
BglII AGATCT 1 cut(s) 897
BisI GCNGC 2 cut(s) 164, 243
BlsI GCNGC 2 cut(s) 165, 244
Bme1390I CCNGG 1 cut(s) 184
Bme18I GGWCC 1 cut(s) 371
BmeT110I CYCGRG 1 cut(s) 546
BmgBI CACGTC 1 cut(s) 806
BmgT120I GGNCC 1 cut(s) 371
BmiI GGNNCC 1 cut(s) 295
BmrFI CCNGG 1 cut(s) 184
BmsI GCATC 2 cut(s) 254, 651
BpiI GAAGAC 1 cut(s) 329
Bpu10I CCTNAGC 1 cut(s) 238
Bsa29I ATCGAT 1 cut(s) 645
BsaWI WCCGGW 2 cut(s) 524, 625
BsaXI ACNNNNNCTCC 2 cut(s) 441, 471
Bsc4I CCNNNNNNNGG 1 cut(s) 386
Bse1I ACTGG 1 cut(s) 758
BseAI TCCGGA 1 cut(s) 524
BseBI CCWGG 1 cut(s) 184
BseCI ATCGAT 1 cut(s) 645
BseGI GGATG 4 cut(s) 49, 245, 622, 748
BseLI CCNNNNNNNGG 1 cut(s) 386
BseMII CTCAG 1 cut(s) 252
BseNI ACTGG 1 cut(s) 758
BseRI GAGGAG 1 cut(s) 139
BseSI GKGCMC 1 cut(s) 849
BseXI GCAGC 2 cut(s) 175, 254
BshFI GGCC 2 cut(s) 426, 446
BshNI GGYRCC 1 cut(s) 293
BshVI ATCGAT 1 cut(s) 645
BsiHKAI GWGCWC 3 cut(s) 553, 849, 858
BsiHKCI CYCGRG 1 cut(s) 546
BsiSI CCGG 3 cut(s) 447, 525, 626
BslFI GGGAC 1 cut(s) 823
BslI CCNNNNNNNGG 1 cut(s) 386
BsmAI GTCTC 3 cut(s) 51, 127, 184
BsmFI GGGAC 1 cut(s) 823
BsnI GGCC 2 cut(s) 426, 446
BsoBI CYCGRG 1 cut(s) 546
Bsp1286I GDGCHC 3 cut(s) 553, 849, 858
Bsp13I TCCGGA 1 cut(s) 524
Bsp143I GATC 4 cut(s) 232, 340, 730, 897
BspACI CCGC 1 cut(s) 480
BspANI GGCC 2 cut(s) 426, 446
BspCNI CTCAG 1 cut(s) 251
BspDI ATCGAT 1 cut(s) 645
BspEI TCCGGA 1 cut(s) 524
BspHI TCATGA 1 cut(s) 405
BspLI GGNNCC 1 cut(s) 295
BspPI GGATC 2 cut(s) 227, 348
BspT107I GGYRCC 1 cut(s) 293
BsrI ACTGG 1 cut(s) 758
BssMI GATC 4 cut(s) 232, 340, 730, 897
Bst2UI CCWGG 1 cut(s) 184
Bst4CI ACNGT 2 cut(s) 178, 658
Bst6I CTCTTC 1 cut(s) 828
BstDEI CTNAG 1 cut(s) 238
BstF5I GGATG 4 cut(s) 49, 245, 622, 748
BstKTI GATC 4 cut(s) 235, 343, 733, 900
BstMAI GTCTC 3 cut(s) 51, 127, 184
BstMBI GATC 4 cut(s) 232, 340, 730, 897
BstMWI GCNNNNNNNGC 2 cut(s) 452, 853
BstNI CCWGG 1 cut(s) 184
BstSCI CCNGG 1 cut(s) 182
BstSLI GKGCMC 1 cut(s) 849
BstV1I GCAGC 2 cut(s) 175, 254
BstV2I GAAGAC 1 cut(s) 329
BstX2I RGATCY 2 cut(s) 340, 897
BstYI RGATCY 2 cut(s) 340, 897
Bsu15I ATCGAT 1 cut(s) 645
BsuRI GGCC 2 cut(s) 426, 446
BsuTUI ATCGAT 1 cut(s) 645
BtrI CACGTC 1 cut(s) 806
BtsCI GGATG 4 cut(s) 49, 245, 622, 748
BtsI GCAGTG 1 cut(s) 789
BtsIMutI CAGTG 2 cut(s) 565, 789
CciI TCATGA 1 cut(s) 405
Cfr13I GGNCC 1 cut(s) 371
ClaI ATCGAT 1 cut(s) 645
CviAII CATG 6 cut(s) 57, 126, 406, 442, 578, 790
DdeI CTNAG 1 cut(s) 238
DpnI GATC 4 cut(s) 234, 342, 732, 899
DpnII GATC 4 cut(s) 232, 340, 730, 897
EaeI YGGCCR 1 cut(s) 444
Eam1104I CTCTTC 1 cut(s) 828
EarI CTCTTC 1 cut(s) 828
Ecl136II GAGCTC 1 cut(s) 551
Eco147I AGGCCT 1 cut(s) 426
Eco24I GRGCYC 1 cut(s) 553
Eco47I GGWCC 1 cut(s) 371
Eco53kI GAGCTC 1 cut(s) 551
Eco57I CTGAAG 3 cut(s) 90, 262, 698
Eco88I CYCGRG 1 cut(s) 546
EcoICRI GAGCTC 1 cut(s) 551
EcoRI GAATTC 1 cut(s) 869
EcoRII CCWGG 1 cut(s) 182
EcoT38I GRGCYC 1 cut(s) 553
FaeI CATG 6 cut(s) 60, 129, 409, 445, 581, 793
FalI AAGNNNNNCTT 2 cut(s) 483, 515
FaqI GGGAC 1 cut(s) 823
FatI CATG 6 cut(s) 56, 125, 405, 441, 577, 789
Fnu4HI GCNGC 2 cut(s) 164, 243
FokI GGATG 4 cut(s) 36, 232, 609, 755
FriOI GRGCYC 1 cut(s) 553
Fsp4HI GCNGC 2 cut(s) 164, 243
FspBI CTAG 3 cut(s) 63, 348, 428
GluI GCNGC 2 cut(s) 164, 243
HaeIII GGCC 2 cut(s) 426, 446
HapII CCGG 3 cut(s) 447, 525, 626
Hin1II CATG 6 cut(s) 60, 129, 409, 445, 581, 793
HindIII AAGCTT 1 cut(s) 221
HinfI GANTC 3 cut(s) 308, 413, 697
HpaII CCGG 3 cut(s) 447, 525, 626
HphI GGTGA 1 cut(s) 449
Hpy166II GTNNAC 1 cut(s) 847
Hpy188I TCNGA 7 cut(s) 229, 281, 313, 418, 702, 735, 797
Hpy188III TCNNGA 5 cut(s) 348, 374, 406, 472, 525
Hpy8I GTNNAC 1 cut(s) 847
HpyAV CCTTC 5 cut(s) 63, 114, 259, 605, 777
HpyCH4III ACNGT 2 cut(s) 178, 658
HpyCH4IV ACGT 1 cut(s) 805
HpyCH4V TGCA 7 cut(s) 35, 71, 119, 189, 642, 782, 847
HpyF10VI GCNNNNNNNGC 2 cut(s) 452, 853
HpyF3I CTNAG 1 cut(s) 238
HpySE526I ACGT 1 cut(s) 805
Hsp92II CATG 6 cut(s) 60, 129, 409, 445, 581, 793
Kpn2I TCCGGA 1 cut(s) 524
Kzo9I GATC 4 cut(s) 232, 340, 730, 897
LmnI GCTCC 1 cut(s) 853
Lsp1109I GCAGC 2 cut(s) 175, 254
LweI GCATC 2 cut(s) 254, 651
MaeI CTAG 3 cut(s) 63, 348, 428
MaeII ACGT 1 cut(s) 805
MaeIII GTNAC 3 cut(s) 172, 437, 560
MalI GATC 4 cut(s) 234, 342, 732, 899
MboI GATC 4 cut(s) 232, 340, 730, 897
MboII GAAGA 6 cut(s) 268, 298, 334, 751, 845, 850
MflI RGATCY 2 cut(s) 340, 897
MhlI GDGCHC 3 cut(s) 553, 849, 858
MluCI AATT 6 cut(s) 265, 316, 554, 685, 705, 869
MmeI TCCRAC 1 cut(s) 810
MnlI CCTC 7 cut(s) 160, 247, 379, 501, 505, 666, 686
MroI TCCGGA 1 cut(s) 524
MseI TTAA 3 cut(s) 98, 219, 617
MspA1I CMGCKG 1 cut(s) 480
MspI CCGG 3 cut(s) 447, 525, 626
MspR9I CCNGG 1 cut(s) 184
MvaI CCWGG 1 cut(s) 184
MwoI GCNNNNNNNGC 2 cut(s) 452, 853
NdeII GATC 4 cut(s) 232, 340, 730, 897
NlaIII CATG 6 cut(s) 60, 129, 409, 445, 581, 793
NlaIV GGNNCC 1 cut(s) 295
NmuCI GTSAC 3 cut(s) 172, 437, 560
PagI TCATGA 1 cut(s) 405
PceI AGGCCT 1 cut(s) 426
PfeI GAWTC 3 cut(s) 308, 413, 697
PkrI GCNGC 2 cut(s) 165, 244
Psp124BI GAGCTC 1 cut(s) 553
Psp6I CCWGG 1 cut(s) 182
PspGI CCWGG 1 cut(s) 182
PspN4I GGNNCC 1 cut(s) 295
PspPI GGNCC 1 cut(s) 371
PsrI GAACNNNNNNTAC 2 cut(s) 770, 802
PsuI RGATCY 2 cut(s) 340, 897
SacI GAGCTC 1 cut(s) 553
SaqAI TTAA 3 cut(s) 98, 219, 617
SatI GCNGC 2 cut(s) 164, 243
Sau3AI GATC 4 cut(s) 232, 340, 730, 897
Sau96I GGNCC 1 cut(s) 371
ScrFI CCNGG 1 cut(s) 184
SduI GDGCHC 3 cut(s) 553, 849, 858
SetI ASST 8 cut(s) 68, 168, 225, 382, 457, 553, 779, 808
SfaNI GCATC 2 cut(s) 254, 651
SinI GGWCC 1 cut(s) 371
Sse9I AATT 6 cut(s) 265, 316, 554, 685, 705, 869
SseBI AGGCCT 1 cut(s) 426
SsiI CCGC 1 cut(s) 480
SspMI CTAG 3 cut(s) 63, 348, 428
SstI GAGCTC 1 cut(s) 553
StuI AGGCCT 1 cut(s) 426
StyD4I CCNGG 1 cut(s) 182
TaaI ACNGT 2 cut(s) 178, 658
TaiI ACGT 1 cut(s) 808
TaqI TCGA 4 cut(s) 306, 506, 645, 664
TasI AATT 6 cut(s) 265, 316, 554, 685, 705, 869
TfiI GAWTC 3 cut(s) 308, 413, 697
Tru1I TTAA 3 cut(s) 98, 219, 617
Tru9I TTAA 3 cut(s) 98, 219, 617
TscAI CASTG 2 cut(s) 565, 789
TseFI GTSAC 3 cut(s) 172, 437, 560
TseI GCWGC 2 cut(s) 163, 242
Tsp45I GTSAC 3 cut(s) 172, 437, 560
TspDTI ATGAA 6 cut(s) 42, 45, 624, 737, 759, 778
TspGWI ACGGA 2 cut(s) 625, 827
TspRI CASTG 2 cut(s) 565, 789
VneI GTGCAC 1 cut(s) 845
VpaK11BI GGWCC 1 cut(s) 371
XapI RAATTY 3 cut(s) 265, 685, 869
XbaI TCTAGA 1 cut(s) 347
XspI CTAG 3 cut(s) 63, 348, 428
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.