pycom03g09160

Plant protein of unknown function (DUF868)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr3
OG0012206
Physical Location & Seq
Forward (+)
7616191 .. 7617600
1410 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom03g09160.2

Sequence Viewer

Length: 1107 bp
ATGATTTTTTTTTCACATCAATTTTTCAATCACAAAACCCAATGGAAAGAATTAAAAGCAGCCATGATCCTGCCACAAATAGTGATGGTACCTGACACTCCTTTGTTAGCCTTTCCTTTCATTTTTCTTTTGAATCTTTTGTCTCGTTTGAACTGGCAAAGCAGCCAAACAAGGCAAAAACTTAGAATTGTTGTTTACACAATGAGAGACAAAGTTGCTTGTTTCAATGAAAATGCAGTAAATGTGTCTCATCCTTCATGTTCTAGGTATGAAAACACTGCTTGTGTTTCTCCAAACTTAACTCCTTCAGTCCAAAATGCAGTCTCATGCTTCTACAAAATCACTCTCTCCTCCACTCAAAAGCAGCTTTTGATCACAGTCTCCTGGTGCAAAAACCACTACGCACAAGGCTTGACCATTAAGCTTTCTGACGATCCCTCAGCAGCATCCTTCAAGTTGAACACGAATTCGCGTATCTTCAGAAAGAAGAAAGGCACCAAAGTCATCGAATCTGATAATTTGAAGACAGAAATCTTTTGGGATCTCTCTAGAGCAAAGTATGATGTTATTGGTCCTGAACCGGTTGAGGGTTTTTACATTTTGGTCATGATTGATTCAGAAATAGGCCTAGTTCTTGGTGACATGGCCGGAGAAGCTGTAAGCAAGAAGTTCAAGACCAGTGGTGTTGCGAAAGCCTCTCTTGTTTCGAGGCAAGAGCATTGTTCCGGAAATGCTCTTTATTCCACCAGAGCTCAATTCAGTGACACAGGCATTGAACATGACATTTTGATAAAATATAGTGGGGAAAATGAAGGCTTAAAACATCCGGTTTTATCCGTTTGCATCGATAAAAAGACGGTCATTCGAGTGAAGAGGCTGCAGTGGAATTTTAGAGGGAATCAGACAATTTTTGTTGATGGGTTGCTTGTTGATCTGATGTGGGATGTTCATAACTGGTTCTTCAATCCTTCATCAAGGTATGCAGTGTTCATGTTCAGAACAAGACGTGGGACGGATAGCAGATTGTGGTTGGAAGAGAAGATGGTGCACAAGGAGGACGACAAAGTTGAATTCTCCTTGTTGATGTATGCCTGTAAGATCTCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF868

Protein Analysis

369

Amino Acids

42.18

Weight (kDa)

9.25

Isoelectric Point (pI)

41.63

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012206)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G27770
fragaria_vesca FvH4_3g24180
malus_domestica MD03G1135700.v1.1 MD11G1158200.v1.1
prunus_persica Prupe.6G120000_v2.0.a1 Prupe.6G120000_v2.0.a1 Prupe.6G120000_v2.0.a1
pyrus_communis pycom03g09160 pycom11g12850
rosa_chinensis RchiOBHm_Chr5g0043291
rosa_laevigata RLG00000034177
rosa_multiflora Rmu_sc0005936.1_g000006
rosa_roxburghii Rroxscaffold_1G00037530
rosa_rugosa Rorug05G0205100
rosa_samantha Rh5BG297100 Rh5CG326900 Rh5DG306700
rosa_wichuraiana Rw5G026880 Rw5G026950

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 88
AccB1I GGYRCC 2 cut(s) 88, 494
AccII CGCG 1 cut(s) 472
AccIII TCCGGA 1 cut(s) 725
AclWI GGATC 3 cut(s) 61, 428, 549
AcoI YGGCCR 1 cut(s) 645
AcsI RAATTY 3 cut(s) 466, 886, 1070
AcuI CTGAAG 2 cut(s) 291, 463
AfaI GTAC 1 cut(s) 90
AfiI CCNNNNNNNGG 1 cut(s) 587
AgeI ACCGGT 1 cut(s) 580
AjiI CACGTC 1 cut(s) 1007
AjnI CCWGG 1 cut(s) 383
AluBI AGCT 4 cut(s) 367, 424, 656, 752
AluI AGCT 4 cut(s) 367, 424, 656, 752
Alw21I GWGCWC 2 cut(s) 754, 1050
Alw26I GTCTC 5 cut(s) 147, 201, 252, 328, 385
Alw44I GTGCAC 1 cut(s) 1046
AlwI GGATC 3 cut(s) 61, 428, 549
Aor13HI TCCGGA 1 cut(s) 725
AoxI GGCC 2 cut(s) 625, 645
ApaLI GTGCAC 1 cut(s) 1046
ApeKI GCWGC 5 cut(s) 59, 162, 364, 443, 877
ApoI RAATTY 3 cut(s) 466, 886, 1070
AsiGI ACCGGT 1 cut(s) 580
Asp718I GGTACC 1 cut(s) 88
AspS9I GGNCC 1 cut(s) 572
AsuHPI GGTGA 1 cut(s) 650
AvaII GGWCC 1 cut(s) 572
BaeGI GKGCMC 1 cut(s) 1050
BanI GGYRCC 2 cut(s) 88, 494
BanII GRGCYC 1 cut(s) 754
BbsI GAAGAC 1 cut(s) 530
Bbv12I GWGCWC 2 cut(s) 754, 1050
BbvCI CCTCAGC 1 cut(s) 439
BbvI GCAGC 5 cut(s) 71, 174, 376, 455, 864
BccI CCATC 3 cut(s) 79, 911, 1036
BciT130I CCWGG 1 cut(s) 385
BclI TGATCA 1 cut(s) 372
BcoDI GTCTC 5 cut(s) 147, 201, 252, 328, 385
BfaI CTAG 3 cut(s) 264, 549, 629
BfmI CTRYAG 1 cut(s) 878
BglII AGATCT 1 cut(s) 1098
BisI GCNGC 5 cut(s) 60, 163, 365, 444, 878
BlsI GCNGC 5 cut(s) 61, 164, 366, 445, 879
Bme1390I CCNGG 1 cut(s) 385
Bme18I GGWCC 1 cut(s) 572
BmgBI CACGTC 1 cut(s) 1007
BmgT120I GGNCC 1 cut(s) 572
BmiI GGNNCC 2 cut(s) 90, 496
BmrFI CCNGG 1 cut(s) 385
BmsI GCATC 2 cut(s) 455, 852
BpiI GAAGAC 1 cut(s) 530
Bpu10I CCTNAGC 1 cut(s) 439
Bsa29I ATCGAT 1 cut(s) 846
BsaWI WCCGGW 3 cut(s) 580, 725, 826
BsaXI ACNNNNNCTCC 2 cut(s) 642, 672
Bsc4I CCNNNNNNNGG 1 cut(s) 587
Bse118I RCCGGY 1 cut(s) 580
Bse1I ACTGG 3 cut(s) 158, 678, 959
BseAI TCCGGA 1 cut(s) 725
BseBI CCWGG 1 cut(s) 385
BseCI ATCGAT 1 cut(s) 846
BseGI GGATG 4 cut(s) 250, 446, 823, 949
BseLI CCNNNNNNNGG 1 cut(s) 587
BseMII CTCAG 1 cut(s) 453
BseNI ACTGG 3 cut(s) 158, 678, 959
BseRI GAGGAG 1 cut(s) 340
BseSI GKGCMC 1 cut(s) 1050
BseXI GCAGC 5 cut(s) 71, 174, 376, 455, 864
Bsh1236I CGCG 1 cut(s) 472
BshFI GGCC 2 cut(s) 627, 647
BshNI GGYRCC 2 cut(s) 88, 494
BshTI ACCGGT 1 cut(s) 580
BshVI ATCGAT 1 cut(s) 846
BsiHKAI GWGCWC 2 cut(s) 754, 1050
BsiSI CCGG 4 cut(s) 581, 648, 726, 827
BslFI GGGAC 1 cut(s) 1024
BslI CCNNNNNNNGG 1 cut(s) 587
BsmAI GTCTC 5 cut(s) 147, 201, 252, 328, 385
BsmFI GGGAC 1 cut(s) 1024
BsnI GGCC 2 cut(s) 627, 647
Bsp1286I GDGCHC 2 cut(s) 754, 1050
Bsp13I TCCGGA 1 cut(s) 725
Bsp143I GATC 6 cut(s) 66, 372, 433, 541, 931, 1098
BspANI GGCC 2 cut(s) 627, 647
BspCNI CTCAG 1 cut(s) 452
BspDI ATCGAT 1 cut(s) 846
BspEI TCCGGA 1 cut(s) 725
BspFNI CGCG 1 cut(s) 472
BspHI TCATGA 1 cut(s) 606
BspLI GGNNCC 2 cut(s) 90, 496
BspMAI CTGCAG 1 cut(s) 882
BspPI GGATC 3 cut(s) 61, 428, 549
BspT107I GGYRCC 2 cut(s) 88, 494
BsrFI RCCGGY 1 cut(s) 580
BsrI ACTGG 3 cut(s) 158, 678, 959
BssAI RCCGGY 1 cut(s) 580
BssMI GATC 6 cut(s) 66, 372, 433, 541, 931, 1098
Bst2UI CCWGG 1 cut(s) 385
Bst4CI ACNGT 2 cut(s) 379, 859
Bst6I CTCTTC 2 cut(s) 866, 1029
BstDEI CTNAG 2 cut(s) 182, 439
BstF5I GGATG 4 cut(s) 250, 446, 823, 949
BstFNI CGCG 1 cut(s) 472
BstKTI GATC 6 cut(s) 69, 375, 436, 544, 934, 1101
BstMAI GTCTC 5 cut(s) 147, 201, 252, 328, 385
BstMBI GATC 6 cut(s) 66, 372, 433, 541, 931, 1098
BstMWI GCNNNNNNNGC 1 cut(s) 653
BstNI CCWGG 1 cut(s) 385
BstSCI CCNGG 1 cut(s) 383
BstSFI CTRYAG 1 cut(s) 878
BstSLI GKGCMC 1 cut(s) 1050
BstUI CGCG 1 cut(s) 472
BstV1I GCAGC 5 cut(s) 71, 174, 376, 455, 864
BstV2I GAAGAC 1 cut(s) 530
BstX2I RGATCY 2 cut(s) 541, 1098
BstYI RGATCY 2 cut(s) 541, 1098
Bsu15I ATCGAT 1 cut(s) 846
BsuRI GGCC 2 cut(s) 627, 647
BsuTUI ATCGAT 1 cut(s) 846
BtrI CACGTC 1 cut(s) 1007
BtsCI GGATG 4 cut(s) 250, 446, 823, 949
BtsI GCAGTG 3 cut(s) 276, 887, 990
BtsIMutI CAGTG 5 cut(s) 276, 685, 766, 887, 990
CciI TCATGA 1 cut(s) 606
Cfr10I RCCGGY 1 cut(s) 580
Cfr13I GGNCC 1 cut(s) 572
ClaI ATCGAT 1 cut(s) 846
Csp6I GTAC 1 cut(s) 89
CspAI ACCGGT 1 cut(s) 580
CspCI CAANNNNNGTGG 2 cut(s) 661, 696
CviAII CATG 7 cut(s) 64, 258, 327, 607, 643, 779, 991
CviQI GTAC 1 cut(s) 89
DdeI CTNAG 2 cut(s) 182, 439
DpnI GATC 6 cut(s) 68, 374, 435, 543, 933, 1100
DpnII GATC 6 cut(s) 66, 372, 433, 541, 931, 1098
EaeI YGGCCR 1 cut(s) 645
Eam1104I CTCTTC 2 cut(s) 866, 1029
EarI CTCTTC 2 cut(s) 866, 1029
Ecl136II GAGCTC 1 cut(s) 752
Eco147I AGGCCT 1 cut(s) 627
Eco24I GRGCYC 1 cut(s) 754
Eco47I GGWCC 1 cut(s) 572
Eco53kI GAGCTC 1 cut(s) 752
Eco57I CTGAAG 2 cut(s) 291, 463
EcoICRI GAGCTC 1 cut(s) 752
EcoRI GAATTC 2 cut(s) 466, 1070
EcoRII CCWGG 1 cut(s) 383
EcoT38I GRGCYC 1 cut(s) 754
FaeI CATG 7 cut(s) 67, 261, 330, 610, 646, 782, 994
FalI AAGNNNNNCTT 2 cut(s) 684, 716
FaqI GGGAC 1 cut(s) 1024
FatI CATG 7 cut(s) 63, 257, 326, 606, 642, 778, 990
FbaI TGATCA 1 cut(s) 372
Fnu4HI GCNGC 5 cut(s) 60, 163, 365, 444, 878
FokI GGATG 4 cut(s) 237, 433, 810, 956
FriOI GRGCYC 1 cut(s) 754
Fsp4HI GCNGC 5 cut(s) 60, 163, 365, 444, 878
FspBI CTAG 3 cut(s) 264, 549, 629
GluI GCNGC 5 cut(s) 60, 163, 365, 444, 878
HaeIII GGCC 2 cut(s) 627, 647
HapII CCGG 4 cut(s) 581, 648, 726, 827
Hin1II CATG 7 cut(s) 67, 261, 330, 610, 646, 782, 994
HindIII AAGCTT 1 cut(s) 422
HinfI GANTC 4 cut(s) 133, 509, 614, 898
HpaII CCGG 4 cut(s) 581, 648, 726, 827
HphI GGTGA 1 cut(s) 650
Hpy166II GTNNAC 2 cut(s) 196, 1048
Hpy188I TCNGA 7 cut(s) 430, 482, 514, 619, 903, 936, 998
Hpy188III TCNNGA 5 cut(s) 549, 575, 607, 673, 726
Hpy8I GTNNAC 2 cut(s) 196, 1048
HpyAV CCTTC 5 cut(s) 264, 315, 460, 806, 978
HpyCH4III ACNGT 2 cut(s) 379, 859
HpyCH4IV ACGT 1 cut(s) 1006
HpyCH4V TGCA 7 cut(s) 236, 320, 390, 843, 880, 983, 1048
HpyF10VI GCNNNNNNNGC 1 cut(s) 653
HpyF3I CTNAG 2 cut(s) 182, 439
HpySE526I ACGT 1 cut(s) 1006
Hsp92II CATG 7 cut(s) 67, 261, 330, 610, 646, 782, 994
Kpn2I TCCGGA 1 cut(s) 725
KpnI GGTACC 1 cut(s) 92
Ksp22I TGATCA 1 cut(s) 372
Kzo9I GATC 6 cut(s) 66, 372, 433, 541, 931, 1098
Lsp1109I GCAGC 5 cut(s) 71, 174, 376, 455, 864
LweI GCATC 2 cut(s) 455, 852
MaeI CTAG 3 cut(s) 264, 549, 629
MaeII ACGT 1 cut(s) 1006
MaeIII GTNAC 2 cut(s) 638, 761
MalI GATC 6 cut(s) 68, 374, 435, 543, 933, 1100
MboI GATC 6 cut(s) 66, 372, 433, 541, 931, 1098
MboII GAAGA 7 cut(s) 469, 499, 535, 883, 952, 1046, 1051
MflI RGATCY 2 cut(s) 541, 1098
MhlI GDGCHC 2 cut(s) 754, 1050
MluCI AATT 9 cut(s) 20, 50, 186, 466, 517, 755, 886, 906, 1070
MmeI TCCRAC 1 cut(s) 1011
MnlI CCTC 8 cut(s) 361, 448, 580, 702, 706, 867, 887, 1048
MroI TCCGGA 1 cut(s) 725
MseI TTAA 4 cut(s) 53, 299, 420, 818
MslI CAYNNNNRTG 1 cut(s) 866
MspI CCGG 4 cut(s) 581, 648, 726, 827
MspR9I CCNGG 1 cut(s) 385
MvaI CCWGG 1 cut(s) 385
MvnI CGCG 1 cut(s) 472
MwoI GCNNNNNNNGC 1 cut(s) 653
NdeII GATC 6 cut(s) 66, 372, 433, 541, 931, 1098
NlaIII CATG 7 cut(s) 67, 261, 330, 610, 646, 782, 994
NlaIV GGNNCC 2 cut(s) 90, 496
NmuCI GTSAC 2 cut(s) 638, 761
PagI TCATGA 1 cut(s) 606
PceI AGGCCT 1 cut(s) 627
PfeI GAWTC 4 cut(s) 133, 509, 614, 898
PinAI ACCGGT 1 cut(s) 580
PkrI GCNGC 5 cut(s) 61, 164, 366, 445, 879
Psp124BI GAGCTC 1 cut(s) 754
Psp6I CCWGG 1 cut(s) 383
PspGI CCWGG 1 cut(s) 383
PspN4I GGNNCC 2 cut(s) 90, 496
PspPI GGNCC 1 cut(s) 572
PsrI GAACNNNNNNTAC 2 cut(s) 971, 1003
PstI CTGCAG 1 cut(s) 882
PsuI RGATCY 2 cut(s) 541, 1098
RsaI GTAC 1 cut(s) 90
RsaNI GTAC 1 cut(s) 89
RseI CAYNNNNRTG 1 cut(s) 866
SacI GAGCTC 1 cut(s) 754
SaqAI TTAA 4 cut(s) 53, 299, 420, 818
SatI GCNGC 5 cut(s) 60, 163, 365, 444, 878
Sau3AI GATC 6 cut(s) 66, 372, 433, 541, 931, 1098
Sau96I GGNCC 1 cut(s) 572
ScrFI CCNGG 1 cut(s) 385
SduI GDGCHC 2 cut(s) 754, 1050
SetI ASST 8 cut(s) 94, 269, 369, 426, 658, 754, 980, 1009
SfaNI GCATC 2 cut(s) 455, 852
SfcI CTRYAG 1 cut(s) 878
SinI GGWCC 1 cut(s) 572
SmiMI CAYNNNNRTG 1 cut(s) 866
Sse9I AATT 9 cut(s) 20, 50, 186, 466, 517, 755, 886, 906, 1070
SseBI AGGCCT 1 cut(s) 627
SspMI CTAG 3 cut(s) 264, 549, 629
SstI GAGCTC 1 cut(s) 754
StuI AGGCCT 1 cut(s) 627
StyD4I CCNGG 1 cut(s) 383
TaaI ACNGT 2 cut(s) 379, 859
TaiI ACGT 1 cut(s) 1009
TaqI TCGA 4 cut(s) 507, 707, 846, 865
TasI AATT 9 cut(s) 20, 50, 186, 466, 517, 755, 886, 906, 1070
TfiI GAWTC 4 cut(s) 133, 509, 614, 898
Tru1I TTAA 4 cut(s) 53, 299, 420, 818
Tru9I TTAA 4 cut(s) 53, 299, 420, 818
TscAI CASTG 5 cut(s) 283, 685, 766, 887, 990
TseFI GTSAC 2 cut(s) 638, 761
TseI GCWGC 5 cut(s) 59, 162, 364, 443, 877
Tsp45I GTSAC 2 cut(s) 638, 761
TspDTI ATGAA 8 cut(s) 109, 243, 246, 285, 825, 938, 960, 979
TspGWI ACGGA 2 cut(s) 826, 1028
TspRI CASTG 5 cut(s) 283, 685, 766, 887, 990
VneI GTGCAC 1 cut(s) 1046
VpaK11BI GGWCC 1 cut(s) 572
XapI RAATTY 3 cut(s) 466, 886, 1070
XbaI TCTAGA 1 cut(s) 548
XspI CTAG 3 cut(s) 264, 549, 629
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.