MD11G1158200.v1.1

Plant protein of unknown function (DUF868)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0012206
Physical Location & Seq
Forward (+)
15383707 .. 15385059
1353 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1158200.v1.1.491

Sequence Viewer

Length: 1068 bp
ATGGTACCATACGGTCCAGACCAGATAAACAAGGCAAGCTTGAAGGTTTTCTGTCTAAATCTTCTACCTCTTTCCTTTCTCAAGATGCAGTTTTCAAGTGGTGCCTGCCTGCCTGCTGGGTTTTGTTTATTGTTTAGAAACTTGGGAATTGTTGTTTACACAATGAGAGACATAGTTTCTTGTTTCAATGAAAATGCAGTGAACGTGTCTCATCCTTCATGTTCTAGCTATGCAAACACTGCTTGTGTTTCTCCAAACTTAACTCCTTCGGTCCAAAATGCAGTCTCGTGTGTCTACAGAATCACTCTCTCCTCCGCTCAAAAGCAGCTCATGGTTACAGTCTCCTGGTGCAAAAACCACTACGTACAAGGGTTGACCATTAAGCTAGCGGATGATCCATCAGCAGCATCCTTCAAGTTGAACACGAATTCGCGTATCTTCCGAAAGAAGAAAGGCACCAAACTCATCGAATCCGATAAATTGAGGATGGAAATCTTTTGGGATCTCTCTAGAGCAAAGTATGATGTTAGTGGTCCTGAACCTGTTGAGGGATTTTACATTTTGGTCTTGGTTGATTCAGAAATAGGCCTAGTTCTAGGTGACATGGCTGAAGAAGCTGTAAGCAAGAAGTTCAAGACTAGTGGTGTTGCGAAAGCCTGTCTTGTTTCGAGGCAAGAGCATTGTTCCGGAAATGCTCTTTATTCTACAAGAGCTCAATTCTGTGACACAGGCATTGCACATGACATTCTGATAAAATGTAGTGGAGAAAATGAAGGCTTAAAACATCCGGTTTTATCTGTTTGCATCGATAAAAAGACGGTGATCCGAGTAAAGAGGCTGCAGTGGAATTTTAGAGGGAATCAGACAATTTTTGTTGATGGGTTGCTTGTTGATCTGATGTGGGATGTTCATAATTGGTTCTTCAATCCTTCATCAAGGTATGCAGTGTTCATGTTCAGAACAAGAAGTGGGATGGACAGCCGATTGTGGTTGGAAGAGAAGATGGTGCACAAAGAGCAGGACAAAGTTGAATTCTCCTTGTTGATGTATGCCTGTAAGAGCTCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF868

Protein Analysis

356

Amino Acids

39.89

Weight (kDa)

8.91

Isoelectric Point (pI)

43.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF868 PF05910 91 - 353 1.1e-90 Plant protein of unknown function (DUF868)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012206)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G27770
fragaria_vesca FvH4_3g24180
malus_domestica MD03G1135700.v1.1 MD11G1158200.v1.1
prunus_persica Prupe.6G120000_v2.0.a1 Prupe.6G120000_v2.0.a1 Prupe.6G120000_v2.0.a1
pyrus_communis pycom03g09160 pycom11g12850
rosa_chinensis RchiOBHm_Chr5g0043291
rosa_laevigata RLG00000034177
rosa_multiflora Rmu_sc0005936.1_g000006
rosa_roxburghii Rroxscaffold_1G00037530
rosa_rugosa Rorug05G0205100
rosa_samantha Rh5BG297100 Rh5CG326900 Rh5DG306700
rosa_wichuraiana Rw5G026880 Rw5G026950

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 4
AccB1I GGYRCC 3 cut(s) 4, 101, 455
AccBSI CCGCTC 1 cut(s) 317
AccI GTMKAC 1 cut(s) 294
AccII CGCG 1 cut(s) 433
AccIII TCCGGA 1 cut(s) 686
AciI CCGC 2 cut(s) 315, 389
AclWI GGATC 3 cut(s) 389, 510, 817
AcsI RAATTY 3 cut(s) 427, 847, 1031
AcuI CTGAAG 1 cut(s) 630
AfaI GTAC 2 cut(s) 6, 366
AfiI CCNNNNNNNGG 1 cut(s) 548
AflIII ACRYGT 1 cut(s) 204
AgsI TTSAA 8 cut(s) 43, 96, 187, 415, 421, 634, 925, 1031
AhlI ACTAGT 1 cut(s) 638
AjnI CCWGG 1 cut(s) 344
AluBI AGCT 7 cut(s) 39, 228, 328, 385, 617, 713, 1062
AluI AGCT 7 cut(s) 39, 228, 328, 385, 617, 713, 1062
Alw21I GWGCWC 3 cut(s) 715, 1011, 1064
Alw26I GTCTC 4 cut(s) 162, 213, 289, 346
Alw44I GTGCAC 1 cut(s) 1007
AlwI GGATC 3 cut(s) 389, 510, 817
Aor13HI TCCGGA 1 cut(s) 686
AoxI GGCC 1 cut(s) 586
ApaLI GTGCAC 1 cut(s) 1007
ApeKI GCWGC 3 cut(s) 325, 404, 838
ApoI RAATTY 3 cut(s) 427, 847, 1031
Asp700I GAANNNNTTC 1 cut(s) 47
Asp718I GGTACC 1 cut(s) 4
AspS9I GGNCC 3 cut(s) 14, 271, 533
AsuHPI GGTGA 2 cut(s) 611, 832
AsuNHI GCTAGC 1 cut(s) 385
AvaII GGWCC 3 cut(s) 14, 271, 533
BaeGI GKGCMC 1 cut(s) 1011
BanI GGYRCC 3 cut(s) 4, 101, 455
BanII GRGCYC 2 cut(s) 715, 1064
BauI CACGAG 1 cut(s) 286
Bbv12I GWGCWC 3 cut(s) 715, 1011, 1064
BbvI GCAGC 3 cut(s) 337, 416, 825
BccI CCATC 5 cut(s) 406, 481, 872, 967, 997
BciT130I CCWGG 1 cut(s) 346
BcoDI GTCTC 4 cut(s) 162, 213, 289, 346
BcuI ACTAGT 1 cut(s) 638
BfaI CTAG 6 cut(s) 225, 386, 510, 590, 596, 639
BfmI CTRYAG 2 cut(s) 295, 839
BisI GCNGC 3 cut(s) 326, 405, 839
BlsI GCNGC 3 cut(s) 327, 406, 840
Bme1390I CCNGG 1 cut(s) 346
Bme18I GGWCC 3 cut(s) 14, 271, 533
BmgT120I GGNCC 3 cut(s) 14, 271, 533
BmiI GGNNCC 3 cut(s) 6, 103, 457
BmrFI CCNGG 1 cut(s) 346
BmsI GCATC 3 cut(s) 75, 416, 813
BmtI GCTAGC 1 cut(s) 389
BpuEI CTTGAG 1 cut(s) 65
Bsa29I ATCGAT 1 cut(s) 807
BsaAI YACGTR 1 cut(s) 364
BsaBI GATNNNNATC 1 cut(s) 491
BsaWI WCCGGW 2 cut(s) 686, 787
Bsc4I CCNNNNNNNGG 1 cut(s) 548
Bse3DI GCAATG 1 cut(s) 732
Bse8I GATNNNNATC 1 cut(s) 491
BseAI TCCGGA 1 cut(s) 686
BseBI CCWGG 1 cut(s) 346
BseCI ATCGAT 1 cut(s) 807
BseGI GGATG 7 cut(s) 211, 397, 407, 492, 784, 910, 978
BseJI GATNNNNATC 1 cut(s) 491
BseLI CCNNNNNNNGG 1 cut(s) 548
BseMI GCAATG 1 cut(s) 732
BseRI GAGGAG 1 cut(s) 301
BseSI GKGCMC 1 cut(s) 1011
BseXI GCAGC 3 cut(s) 337, 416, 825
BseYI CCCAGC 1 cut(s) 116
Bsh1236I CGCG 1 cut(s) 433
BshFI GGCC 1 cut(s) 588
BshNI GGYRCC 3 cut(s) 4, 101, 455
BshVI ATCGAT 1 cut(s) 807
BsiHKAI GWGCWC 3 cut(s) 715, 1011, 1064
BsiSI CCGG 2 cut(s) 687, 788
BslI CCNNNNNNNGG 1 cut(s) 548
BsmAI GTCTC 4 cut(s) 162, 213, 289, 346
BsnI GGCC 1 cut(s) 588
Bsp1286I GDGCHC 3 cut(s) 715, 1011, 1064
Bsp13I TCCGGA 1 cut(s) 686
Bsp143I GATC 4 cut(s) 394, 502, 822, 892
BspACI CCGC 2 cut(s) 315, 389
BspANI GGCC 1 cut(s) 588
BspDI ATCGAT 1 cut(s) 807
BspEI TCCGGA 1 cut(s) 686
BspFNI CGCG 1 cut(s) 433
BspLI GGNNCC 3 cut(s) 6, 103, 457
BspMAI CTGCAG 1 cut(s) 843
BspOI GCTAGC 1 cut(s) 389
BspPI GGATC 3 cut(s) 389, 510, 817
BspT107I GGYRCC 3 cut(s) 4, 101, 455
BsrBI CCGCTC 1 cut(s) 317
BsrDI GCAATG 1 cut(s) 732
BssMI GATC 4 cut(s) 394, 502, 822, 892
BssSI CACGAG 1 cut(s) 286
Bst2BI CACGAG 1 cut(s) 286
Bst2UI CCWGG 1 cut(s) 346
Bst4CI ACNGT 3 cut(s) 14, 340, 820
Bst6I CTCTTC 1 cut(s) 990
BstAPI GCANNNNNTGC 1 cut(s) 239
BstBAI YACGTR 1 cut(s) 364
BstC8I GCNNGC 5 cut(s) 37, 106, 110, 114, 387
BstF5I GGATG 7 cut(s) 211, 397, 407, 492, 784, 910, 978
BstFNI CGCG 1 cut(s) 433
BstKTI GATC 4 cut(s) 397, 505, 825, 895
BstMAI GTCTC 4 cut(s) 162, 213, 289, 346
BstMBI GATC 4 cut(s) 394, 502, 822, 892
BstMWI GCNNNNNNNGC 3 cut(s) 239, 614, 1015
BstNI CCWGG 1 cut(s) 346
BstSCI CCNGG 1 cut(s) 344
BstSFI CTRYAG 2 cut(s) 295, 839
BstSLI GKGCMC 1 cut(s) 1011
BstSNI TACGTA 1 cut(s) 364
BstUI CGCG 1 cut(s) 433
BstV1I GCAGC 3 cut(s) 337, 416, 825
BstX2I RGATCY 1 cut(s) 502
BstYI RGATCY 1 cut(s) 502
Bsu15I ATCGAT 1 cut(s) 807
BsuRI GGCC 1 cut(s) 588
BsuTUI ATCGAT 1 cut(s) 807
BtsCI GGATG 7 cut(s) 211, 397, 407, 492, 784, 910, 978
BtsI GCAGTG 4 cut(s) 204, 237, 848, 951
BtsIMutI CAGTG 4 cut(s) 204, 237, 848, 951
Cac8I GCNNGC 5 cut(s) 37, 106, 110, 114, 387
Cfr13I GGNCC 3 cut(s) 14, 271, 533
ClaI ATCGAT 1 cut(s) 807
Csp6I GTAC 2 cut(s) 5, 365
CspCI CAANNNNNGTGG 2 cut(s) 622, 657
CviAII CATG 5 cut(s) 219, 331, 604, 740, 952
CviQI GTAC 2 cut(s) 5, 365
DpnI GATC 4 cut(s) 396, 504, 824, 894
DpnII GATC 4 cut(s) 394, 502, 822, 892
Eam1104I CTCTTC 1 cut(s) 990
EarI CTCTTC 1 cut(s) 990
Ecl136II GAGCTC 2 cut(s) 713, 1062
Eco105I TACGTA 1 cut(s) 364
Eco147I AGGCCT 1 cut(s) 588
Eco24I GRGCYC 2 cut(s) 715, 1064
Eco47I GGWCC 3 cut(s) 14, 271, 533
Eco53kI GAGCTC 2 cut(s) 713, 1062
Eco57I CTGAAG 1 cut(s) 630
EcoICRI GAGCTC 2 cut(s) 713, 1062
EcoRI GAATTC 2 cut(s) 427, 1031
EcoRII CCWGG 1 cut(s) 344
EcoT38I GRGCYC 2 cut(s) 715, 1064
FaeI CATG 5 cut(s) 222, 334, 607, 743, 955
FalI AAGNNNNNCTT 4 cut(s) 23, 55, 645, 677
FatI CATG 5 cut(s) 218, 330, 603, 739, 951
FblI GTMKAC 1 cut(s) 294
Fnu4HI GCNGC 3 cut(s) 326, 405, 839
FokI GGATG 7 cut(s) 198, 394, 404, 499, 771, 917, 985
FriOI GRGCYC 2 cut(s) 715, 1064
Fsp4HI GCNGC 3 cut(s) 326, 405, 839
FspBI CTAG 6 cut(s) 225, 386, 510, 590, 596, 639
GluI GCNGC 3 cut(s) 326, 405, 839
GsaI CCCAGC 1 cut(s) 120
HaeIII GGCC 1 cut(s) 588
HapII CCGG 2 cut(s) 687, 788
Hin1II CATG 5 cut(s) 222, 334, 607, 743, 955
HincII GTYRAC 1 cut(s) 375
HindII GTYRAC 1 cut(s) 375
HindIII AAGCTT 1 cut(s) 37
HinfI GANTC 4 cut(s) 300, 470, 575, 859
HpaII CCGG 2 cut(s) 687, 788
HphI GGTGA 2 cut(s) 611, 832
Hpy166II GTNNAC 5 cut(s) 157, 202, 295, 375, 1009
Hpy188I TCNGA 8 cut(s) 443, 475, 580, 750, 827, 864, 897, 959
Hpy188III TCNNGA 6 cut(s) 17, 82, 510, 536, 634, 687
Hpy8I GTNNAC 5 cut(s) 157, 202, 295, 375, 1009
HpyAV CCTTC 6 cut(s) 37, 225, 276, 421, 767, 939
HpyCH4III ACNGT 3 cut(s) 14, 340, 820
HpyCH4IV ACGT 2 cut(s) 204, 363
HpyF10VI GCNNNNNNNGC 3 cut(s) 239, 614, 1015
HpySE526I ACGT 2 cut(s) 204, 363
Hsp92II CATG 5 cut(s) 222, 334, 607, 743, 955
Kpn2I TCCGGA 1 cut(s) 686
KpnI GGTACC 1 cut(s) 8
Kzo9I GATC 4 cut(s) 394, 502, 822, 892
Lsp1109I GCAGC 3 cut(s) 337, 416, 825
LweI GCATC 3 cut(s) 75, 416, 813
MaeI CTAG 6 cut(s) 225, 386, 510, 590, 596, 639
MaeII ACGT 2 cut(s) 204, 363
MaeIII GTNAC 3 cut(s) 334, 599, 722
MalI GATC 4 cut(s) 396, 504, 824, 894
MbiI CCGCTC 1 cut(s) 317
MboI GATC 4 cut(s) 394, 502, 822, 892
MboII GAAGA 7 cut(s) 53, 430, 460, 623, 913, 1007, 1012
MflI RGATCY 1 cut(s) 502
MhlI GDGCHC 3 cut(s) 715, 1011, 1064
MluCI AATT 8 cut(s) 147, 427, 479, 716, 847, 867, 913, 1031
MmeI TCCRAC 1 cut(s) 972
MnlI CCTC 7 cut(s) 78, 322, 477, 541, 663, 828, 848
MroI TCCGGA 1 cut(s) 686
MroXI GAANNNNTTC 1 cut(s) 47
MseI TTAA 4 cut(s) 260, 381, 779, 1066
MspI CCGG 2 cut(s) 687, 788
MspR9I CCNGG 1 cut(s) 346
MvaI CCWGG 1 cut(s) 346
MvnI CGCG 1 cut(s) 433
MwoI GCNNNNNNNGC 3 cut(s) 239, 614, 1015
NdeII GATC 4 cut(s) 394, 502, 822, 892
NheI GCTAGC 1 cut(s) 385
NlaIII CATG 5 cut(s) 222, 334, 607, 743, 955
NlaIV GGNNCC 3 cut(s) 6, 103, 457
NmuCI GTSAC 2 cut(s) 599, 722
PceI AGGCCT 1 cut(s) 588
PdmI GAANNNNTTC 1 cut(s) 47
PfeI GAWTC 4 cut(s) 300, 470, 575, 859
PkrI GCNGC 3 cut(s) 327, 406, 840
Ppu21I YACGTR 1 cut(s) 364
Psp124BI GAGCTC 2 cut(s) 715, 1064
Psp6I CCWGG 1 cut(s) 344
PspFI CCCAGC 1 cut(s) 116
PspGI CCWGG 1 cut(s) 344
PspN4I GGNNCC 3 cut(s) 6, 103, 457
PspPI GGNCC 3 cut(s) 14, 271, 533
PsrI GAACNNNNNNTAC 2 cut(s) 932, 964
PstI CTGCAG 1 cut(s) 843
PsuI RGATCY 1 cut(s) 502
RsaI GTAC 2 cut(s) 6, 366
RsaNI GTAC 2 cut(s) 5, 365
SacI GAGCTC 2 cut(s) 715, 1064
SaqAI TTAA 4 cut(s) 260, 381, 779, 1066
SatI GCNGC 3 cut(s) 326, 405, 839
Sau3AI GATC 4 cut(s) 394, 502, 822, 892
Sau96I GGNCC 3 cut(s) 14, 271, 533
ScrFI CCNGG 1 cut(s) 346
SduI GDGCHC 3 cut(s) 715, 1011, 1064
SfaNI GCATC 3 cut(s) 75, 416, 813
SfcI CTRYAG 2 cut(s) 295, 839
SinI GGWCC 3 cut(s) 14, 271, 533
SmlI CTYRAG 1 cut(s) 80
SmoI CTYRAG 1 cut(s) 80
SnaBI TACGTA 1 cut(s) 364
SpeI ACTAGT 1 cut(s) 638
Sse9I AATT 8 cut(s) 147, 427, 479, 716, 847, 867, 913, 1031
SseBI AGGCCT 1 cut(s) 588
SsiI CCGC 2 cut(s) 315, 389
SspMI CTAG 6 cut(s) 225, 386, 510, 590, 596, 639
SstI GAGCTC 2 cut(s) 715, 1064
StuI AGGCCT 1 cut(s) 588
StyD4I CCNGG 1 cut(s) 344
TaaI ACNGT 3 cut(s) 14, 340, 820
TaiI ACGT 2 cut(s) 207, 366
TaqI TCGA 3 cut(s) 468, 668, 807
TaqII GACCGA 1 cut(s) 259
TasI AATT 8 cut(s) 147, 427, 479, 716, 847, 867, 913, 1031
TfiI GAWTC 4 cut(s) 300, 470, 575, 859
Tru1I TTAA 4 cut(s) 260, 381, 779, 1066
Tru9I TTAA 4 cut(s) 260, 381, 779, 1066
TscAI CASTG 4 cut(s) 204, 244, 848, 951
TseFI GTSAC 2 cut(s) 599, 722
TseI GCWGC 3 cut(s) 325, 404, 838
Tsp45I GTSAC 2 cut(s) 599, 722
TspDTI ATGAA 6 cut(s) 204, 207, 786, 899, 921, 940
TspRI CASTG 4 cut(s) 204, 244, 848, 951
VneI GTGCAC 1 cut(s) 1007
VpaK11BI GGWCC 3 cut(s) 14, 271, 533
XapI RAATTY 3 cut(s) 427, 847, 1031
XbaI TCTAGA 1 cut(s) 509
XmiI GTMKAC 1 cut(s) 294
XmnI GAANNNNTTC 1 cut(s) 47
XspI CTAG 6 cut(s) 225, 386, 510, 590, 596, 639
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.