MD03G1137900.v1.1
ERF Family

Belongs to the protein kinase superfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0010356
Physical Location & Seq
Forward (+)
14077916 .. 14084479
6564 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1137900.v1.1.491

Sequence Viewer

Length: 1380 bp
ATGGAGCCGTGGCTTGACGACTTAGCCGACGACCTCCAAAGCCTGAGCTTCACGTCCACCACAACCACCAACACCAACACTACCAAGCGCAGCACTAGCTCCTGCTCCGAATCCACGCACCCGCCCTCCGCCTCCTCCTCCGCTCGCCTCCCCGCCTCCGTAATCCCCAAGCCCCGACCCCAATCCTCCTCCTCCGACCCCCTCTGGTCCGCAATCTACAAGATCCGATCCGAATCCCCGACCCGGCAACTCTCCCTCTCCGACCTCAAATTCGCAGAGCGCCTCGGCTCCGGCGACATCGGTTCCGTCTACTTAGCCGAGTTGAAGTCCCCCGCTGGGGCCCAGGGGTGCGTATTTGCGGCGAAGGTGATGGACAAGAAGGAGCTGATCAGCCGGAGCAAGGAAGGGCGGGCCAGGACAGAGAGGGAAATACTGGAAATGTTGGAGCACCCTTTTTTGCCCACGCTCTACGCAAGCATCGACGCGCCCAACTGGCTTTTCCTGCTCACCGAGTTCTGCCCCGGCGGCGACCTCCACGTTCTCCGCCAGCGCCAGCCGCTCAAACGCTTCGAAGAACCTGCCGTCAGGTTCTACGCATCGGAGGTGGTTGTAGCTCTAGAGTACCTCCACATGATGGGAATTGTGTACCGTGATCTGAAGCCGGAAAATGTGTTGGTTCGATCCGATGGCCATATCATGCTTACAGATTTTGACCTCTCGTTAAAATGCGACGATGGTACATCAACGCCTCAGATCATCTCTACCCAAAATACCCCAAATGCTGCACTACAAAAAGGCTACTCACTCGATCCGCCCCCGTTTGCATCCTCTTCATGCATTCTTCCCAGTTGTATGGTCCCCTCCGTGTCATGTTTCCACCCGAAAAGCAAGCGGAAGAAGAAGCAGAGCCATAAAAATGGCCCCGAGTTTGTGTCCGAGCCCGTGGACGTTCGGTCCATGTCGTTTGTCGGGACCCACGAGTACTTGGCCCCGGAGATTGTGTCCGGCGAGGGGCATGGTAGTGCAGTGGACTGGTGGACGTTAGGGATATTTATTTTTGAACTGTTCTATGGTGTGACACCCTTTAGGGGTGTGGACAATGAGCTAACCCTAGCTAACATTGTGGCTCGAGCCCTGGAGCTTCCGAAAGAACCGGCGGTGCCGAGCACGGCCAAGGACCTCATCTCACAGTTGTTGGTCAAGGATCCGGCTCGGCGTTTGGGATCGTTGATGGGTGCATCAGCAGTCAAGAGGCACCCATTTTTCCAAGGGGTTAATTGGGCATTGTTGAGATGTACACCTCCACCATTTATTCCCCCACCATTTAGTAGACGAGTTGTATCTGATCAAAGTTGTACTGAGAAAATGGAATATTATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pkinase

Protein Analysis

460

Amino Acids

50.8

Weight (kDa)

6.8

Isoelectric Point (pI)

62.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PK_Tyr_Ser-Thr PF07714 89 - 244 3.9e-19 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 91 - 256 3.6e-33 Protein kinase domain
Pkinase PF00069 319 - 422 4.6e-20 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 327 - 406 1.8e-06 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 586
AccB1I GGYRCC 2 cut(s) 1159, 1254
AccB7I CCANNNNNTGG 1 cut(s) 634
AccBSI CCGCTC 2 cut(s) 143, 559
AccI GTMKAC 2 cut(s) 309, 1330
AccII CGCG 1 cut(s) 485
AclWI GGATC 7 cut(s) 217, 222, 675, 803, 1199, 1212, 1231
AcoI YGGCCR 2 cut(s) 688, 1170
AcsI RAATTY 1 cut(s) 269
AcuI CTGAAG 1 cut(s) 677
AfaI GTAC 6 cut(s) 623, 647, 739, 983, 1297, 1357
AfiI CCNNNNNNNGG 7 cut(s) 243, 336, 337, 400, 634, 1011, 1088
AgsI TTSAA 2 cut(s) 325, 1061
AhdI GACNNNNNGTC 1 cut(s) 952
AjiI CACGTC 1 cut(s) 54
AjnI CCWGG 3 cut(s) 342, 413, 1134
AjuI GAANNNNNNNTTGG 2 cut(s) 482, 514
AluBI AGCT 7 cut(s) 48, 99, 385, 614, 1105, 1115, 1141
AluI AGCT 7 cut(s) 48, 99, 385, 614, 1105, 1115, 1141
Alw21I GWGCWC 2 cut(s) 450, 1169
AlwI GGATC 7 cut(s) 217, 222, 675, 803, 1199, 1212, 1231
Ama87I CYCGRG 2 cut(s) 923, 1128
AoxI GGCC 6 cut(s) 339, 411, 688, 919, 987, 1170
ApaI GGGCCC 1 cut(s) 343
ApeKI GCWGC 2 cut(s) 90, 782
ApoI RAATTY 1 cut(s) 269
ArsI GACNNNNNNTTYG 2 cut(s) 254, 286
AspLEI GCGC 4 cut(s) 90, 282, 487, 552
AsuC2I CCSGG 3 cut(s) 244, 522, 992
AsuHPI GGTGA 2 cut(s) 379, 499
AsuII TTCGAA 1 cut(s) 570
AvaI CYCGRG 2 cut(s) 923, 1128
AvaII GGWCC 5 cut(s) 207, 856, 954, 972, 1177
BaeGI GKGCMC 1 cut(s) 343
BalI TGGCCA 1 cut(s) 690
BamHI GGATCC 1 cut(s) 1204
BanI GGYRCC 2 cut(s) 1159, 1254
BanII GRGCYC 3 cut(s) 343, 942, 1135
BauI CACGAG 1 cut(s) 977
Bbv12I GWGCWC 2 cut(s) 450, 1169
BbvI GCAGC 2 cut(s) 102, 769
BccI CCATC 5 cut(s) 364, 628, 680, 728, 1225
BceAI ACGGC 2 cut(s) 566, 1185
BcgI CGANNNNNNTGC 2 cut(s) 560, 594
BciT130I CCWGG 3 cut(s) 344, 415, 1136
BclI TGATCA 2 cut(s) 387, 1345
BcnI CCSGG 3 cut(s) 244, 522, 992
BfaI CTAG 3 cut(s) 96, 617, 1112
BfoI RGCGCY 2 cut(s) 283, 553
BfuAI ACCTGC 1 cut(s) 586
BglI GCCNNNNNGGC 2 cut(s) 493, 525
BisI GCNGC 5 cut(s) 91, 360, 526, 557, 783
BlsI GCNGC 5 cut(s) 92, 361, 527, 558, 784
BmcAI AGTACT 1 cut(s) 983
Bme1390I CCNGG 6 cut(s) 244, 344, 415, 522, 992, 1136
Bme18I GGWCC 5 cut(s) 207, 856, 954, 972, 1177
BmeRI GACNNNNNGTC 1 cut(s) 952
BmeT110I CYCGRG 2 cut(s) 923, 1128
BmgBI CACGTC 1 cut(s) 54
BmrFI CCNGG 6 cut(s) 244, 344, 415, 522, 992, 1136
BmrI ACTGGG 1 cut(s) 840
BmsI GCATC 4 cut(s) 486, 605, 833, 1247
BmuI ACTGGG 1 cut(s) 840
BpmI CTGGAG 1 cut(s) 1157
Bpu10I CCTNAGC 1 cut(s) 44
Bpu14I TTCGAA 1 cut(s) 570
BpuMI CCSGG 3 cut(s) 244, 522, 992
BsaBI GATNNNNATC 1 cut(s) 232
BsaXI ACNNNNNCTCC 4 cut(s) 110, 140, 986, 1016
Bsc4I CCNNNNNNNGG 7 cut(s) 243, 336, 337, 400, 634, 1011, 1088
Bse118I RCCGGY 1 cut(s) 1153
Bse1I ACTGG 4 cut(s) 438, 497, 846, 1037
Bse8I GATNNNNATC 1 cut(s) 232
BseBI CCWGG 3 cut(s) 344, 415, 1136
BseGI GGATG 1 cut(s) 824
BseJI GATNNNNATC 1 cut(s) 232
BseLI CCNNNNNNNGG 7 cut(s) 243, 336, 337, 400, 634, 1011, 1088
BseMII CTCAG 3 cut(s) 35, 764, 1350
BseNI ACTGG 4 cut(s) 438, 497, 846, 1037
BseRI GAGGAG 4 cut(s) 124, 127, 178, 181
BseSI GKGCMC 1 cut(s) 343
BseXI GCAGC 2 cut(s) 102, 769
BseYI CCCAGC 1 cut(s) 335
BsgI GTGCAG 2 cut(s) 768, 1044
Bsh1236I CGCG 1 cut(s) 485
BshFI GGCC 6 cut(s) 341, 413, 690, 921, 989, 1172
BshNI GGYRCC 2 cut(s) 1159, 1254
BsiHKAI GWGCWC 2 cut(s) 450, 1169
BsiHKCI CYCGRG 2 cut(s) 923, 1128
BsiSI CCGG 9 cut(s) 244, 291, 394, 522, 662, 992, 1005, 1154, 1208
BslFI GGGAC 3 cut(s) 313, 842, 985
BslI CCNNNNNNNGG 7 cut(s) 243, 336, 337, 400, 634, 1011, 1088
BsmFI GGGAC 3 cut(s) 313, 842, 985
BsmI GAATGC 1 cut(s) 837
BsnI GGCC 6 cut(s) 341, 413, 690, 921, 989, 1172
BsoBI CYCGRG 2 cut(s) 923, 1128
Bsp119I TTCGAA 1 cut(s) 570
Bsp120I GGGCCC 1 cut(s) 339
Bsp1286I GDGCHC 5 cut(s) 343, 450, 942, 1135, 1169
Bsp1407I TGTACA 1 cut(s) 1295
BspANI GGCC 6 cut(s) 341, 413, 690, 921, 989, 1172
BspCNI CTCAG 3 cut(s) 36, 763, 1351
BspFNI CGCG 1 cut(s) 485
BspMI ACCTGC 1 cut(s) 586
BspPI GGATC 7 cut(s) 217, 222, 675, 803, 1199, 1212, 1231
BspT104I TTCGAA 1 cut(s) 570
BspT107I GGYRCC 2 cut(s) 1159, 1254
BsrBI CCGCTC 2 cut(s) 143, 559
BsrFI RCCGGY 1 cut(s) 1153
BsrGI TGTACA 1 cut(s) 1295
BsrI ACTGG 4 cut(s) 438, 497, 846, 1037
BssAI RCCGGY 1 cut(s) 1153
BssSI CACGAG 1 cut(s) 977
BssT1I CCWWGG 2 cut(s) 1173, 1267
Bst2BI CACGAG 1 cut(s) 977
Bst2UI CCWGG 3 cut(s) 344, 415, 1136
Bst4CI ACNGT 3 cut(s) 650, 1065, 1191
Bst6I CTCTTC 1 cut(s) 835
BstAUI TGTACA 1 cut(s) 1295
BstBI TTCGAA 1 cut(s) 570
BstC8I GCNNGC 6 cut(s) 145, 411, 475, 548, 554, 890
BstDEI CTNAG 5 cut(s) 22, 44, 313, 750, 1359
BstDSI CCRYGG 2 cut(s) 8, 942
BstF5I GGATG 1 cut(s) 824
BstFNI CGCG 1 cut(s) 485
BstH2I RGCGCY 2 cut(s) 283, 553
BstHHI GCGC 4 cut(s) 90, 282, 487, 552
BstMWI GCNNNNNNNGC 5 cut(s) 96, 493, 502, 525, 556
BstNI CCWGG 3 cut(s) 344, 415, 1136
BstSCI CCNGG 6 cut(s) 242, 342, 413, 520, 990, 1134
BstSLI GKGCMC 1 cut(s) 343
BstUI CGCG 1 cut(s) 485
BstV1I GCAGC 2 cut(s) 102, 769
BstX2I RGATCY 2 cut(s) 222, 1204
BstXI CCANNNNNNTGG 2 cut(s) 853, 917
BstYI RGATCY 2 cut(s) 222, 1204
BsuRI GGCC 6 cut(s) 341, 413, 690, 921, 989, 1172
BtgI CCRYGG 2 cut(s) 8, 942
BtrI CACGTC 1 cut(s) 54
BtsCI GGATG 1 cut(s) 824
BtsI GCAGTG 1 cut(s) 1032
BtsIMutI CAGTG 1 cut(s) 1032
BveI ACCTGC 1 cut(s) 586
Cac8I GCNNGC 6 cut(s) 145, 411, 475, 548, 554, 890
CfoI GCGC 4 cut(s) 90, 282, 487, 552
Cfr10I RCCGGY 1 cut(s) 1153
CseI GACGC 1 cut(s) 491
Csp6I GTAC 6 cut(s) 622, 646, 738, 982, 1296, 1356
CviAII CATG 6 cut(s) 631, 697, 834, 870, 958, 1016
CviQI GTAC 6 cut(s) 622, 646, 738, 982, 1296, 1356
DdeI CTNAG 5 cut(s) 22, 44, 313, 750, 1359
DriI GACNNNNNGTC 1 cut(s) 952
EaeI YGGCCR 2 cut(s) 688, 1170
Eam1104I CTCTTC 1 cut(s) 835
Eam1105I GACNNNNNGTC 1 cut(s) 952
EarI CTCTTC 1 cut(s) 835
EciI GGCGGA 3 cut(s) 118, 533, 801
Eco130I CCWWGG 2 cut(s) 1173, 1267
Eco24I GRGCYC 3 cut(s) 343, 942, 1135
Eco47I GGWCC 5 cut(s) 207, 856, 954, 972, 1177
Eco57I CTGAAG 1 cut(s) 677
Eco88I CYCGRG 2 cut(s) 923, 1128
EcoO109I RGGNCCY 3 cut(s) 339, 972, 1177
EcoRII CCWGG 3 cut(s) 342, 413, 1134
EcoT14I CCWWGG 2 cut(s) 1173, 1267
EcoT22I ATGCAT 1 cut(s) 839
EcoT38I GRGCYC 3 cut(s) 343, 942, 1135
ErhI CCWWGG 2 cut(s) 1173, 1267
FaeI CATG 6 cut(s) 634, 700, 837, 873, 961, 1019
FaqI GGGAC 3 cut(s) 313, 842, 985
FatI CATG 6 cut(s) 630, 696, 833, 869, 957, 1015
FauI CCCGC 4 cut(s) 129, 160, 340, 402
FbaI TGATCA 2 cut(s) 387, 1345
FblI GTMKAC 2 cut(s) 309, 1330
Fnu4HI GCNGC 5 cut(s) 91, 360, 526, 557, 783
FokI GGATG 1 cut(s) 811
FriOI GRGCYC 3 cut(s) 343, 942, 1135
Fsp4HI GCNGC 5 cut(s) 91, 360, 526, 557, 783
FspBI CTAG 3 cut(s) 96, 617, 1112
GlaI GCGC 4 cut(s) 89, 281, 486, 551
GluI GCNGC 5 cut(s) 91, 360, 526, 557, 783
GsaI CCCAGC 1 cut(s) 339
GsuI CTGGAG 1 cut(s) 1157
HaeII RGCGCY 2 cut(s) 283, 553
HaeIII GGCC 6 cut(s) 341, 413, 690, 921, 989, 1172
HapII CCGG 9 cut(s) 244, 291, 394, 522, 662, 992, 1005, 1154, 1208
HgaI GACGC 1 cut(s) 491
HhaI GCGC 4 cut(s) 90, 282, 487, 552
Hin1II CATG 6 cut(s) 634, 700, 837, 873, 961, 1019
Hin6I GCGC 4 cut(s) 88, 280, 485, 550
HinP1I GCGC 4 cut(s) 88, 280, 485, 550
HinfI GANTC 2 cut(s) 110, 233
HpaII CCGG 9 cut(s) 244, 291, 394, 522, 662, 992, 1005, 1154, 1208
HphI GGTGA 2 cut(s) 379, 499
Hpy166II GTNNAC 9 cut(s) 57, 310, 646, 946, 1030, 1038, 1096, 1298, 1331
Hpy188III TCNNGA 3 cut(s) 617, 970, 1249
Hpy8I GTNNAC 9 cut(s) 57, 310, 646, 946, 1030, 1038, 1096, 1298, 1331
Hpy99I CGWCG 3 cut(s) 32, 485, 734
HpyAV CCTTC 3 cut(s) 358, 373, 398
HpyCH4III ACNGT 3 cut(s) 650, 1065, 1191
HpyCH4IV ACGT 4 cut(s) 53, 537, 948, 1040
HpyCH4V TGCA 5 cut(s) 785, 824, 837, 1025, 1238
HpyF10VI GCNNNNNNNGC 5 cut(s) 96, 493, 502, 525, 556
HpyF3I CTNAG 5 cut(s) 22, 44, 313, 750, 1359
HpySE526I ACGT 4 cut(s) 53, 537, 948, 1040
Hsp92II CATG 6 cut(s) 634, 700, 837, 873, 961, 1019
HspAI GCGC 4 cut(s) 88, 280, 485, 550
KflI GGGWCCC 1 cut(s) 972
Ksp22I TGATCA 2 cut(s) 387, 1345
LmnI GCTCC 8 cut(s) 4, 104, 110, 293, 382, 396, 445, 1138
Lsp1109I GCAGC 2 cut(s) 102, 769
LweI GCATC 4 cut(s) 486, 605, 833, 1247
MaeI CTAG 3 cut(s) 96, 617, 1112
MaeII ACGT 4 cut(s) 53, 537, 948, 1040
MaeIII GTNAC 1 cut(s) 1075
MbiI CCGCTC 2 cut(s) 143, 559
MboII GAAGA 5 cut(s) 584, 822, 833, 907, 910
MflI RGATCY 2 cut(s) 222, 1204
MhlI GDGCHC 5 cut(s) 343, 450, 942, 1135, 1169
MlsI TGGCCA 1 cut(s) 690
MluCI AATT 3 cut(s) 269, 639, 1276
MluNI TGGCCA 1 cut(s) 690
MmeI TCCRAC 3 cut(s) 219, 285, 423
Mox20I TGGCCA 1 cut(s) 690
Mph1103I ATGCAT 1 cut(s) 839
MscI TGGCCA 1 cut(s) 690
MseI TTAA 2 cut(s) 722, 1275
MslI CAYNNNNRTG 2 cut(s) 915, 1020
Msp20I TGGCCA 1 cut(s) 690
MspA1I CMGCKG 1 cut(s) 335
MspI CCGG 9 cut(s) 244, 291, 394, 522, 662, 992, 1005, 1154, 1208
MspR9I CCNGG 6 cut(s) 244, 344, 415, 522, 992, 1136
Mva1269I GAATGC 1 cut(s) 837
MvaI CCWGG 3 cut(s) 344, 415, 1136
MvnI CGCG 1 cut(s) 485
MwoI GCNNNNNNNGC 5 cut(s) 96, 493, 502, 525, 556
NciI CCSGG 3 cut(s) 244, 522, 992
NlaIII CATG 6 cut(s) 634, 700, 837, 873, 961, 1019
NmeAIII GCCGAG 4 cut(s) 264, 343, 1188, 1192
NmuCI GTSAC 1 cut(s) 1075
NsiI ATGCAT 1 cut(s) 839
NspV TTCGAA 1 cut(s) 570
PaeR7I CTCGAG 1 cut(s) 1128
PasI CCCWGGG 1 cut(s) 343
PcsI WCGNNNNNNNCGW 4 cut(s) 24, 291, 477, 975
PctI GAATGC 1 cut(s) 837
PfeI GAWTC 2 cut(s) 110, 233
PflMI CCANNNNNTGG 1 cut(s) 634
PkrI GCNGC 5 cut(s) 92, 361, 527, 558, 784
PpuMI RGGWCCY 2 cut(s) 972, 1177
Psp5II RGGWCCY 2 cut(s) 972, 1177
Psp6I CCWGG 3 cut(s) 342, 413, 1134
PspFI CCCAGC 1 cut(s) 335
PspGI CCWGG 3 cut(s) 342, 413, 1134
PspOMI GGGCCC 1 cut(s) 339
PspPPI RGGWCCY 2 cut(s) 972, 1177
PspXI VCTCGAGB 1 cut(s) 1128
PsuI RGATCY 2 cut(s) 222, 1204
RsaI GTAC 6 cut(s) 623, 647, 739, 983, 1297, 1357
RsaNI GTAC 6 cut(s) 622, 646, 738, 982, 1296, 1356
RseI CAYNNNNRTG 2 cut(s) 915, 1020
SaqAI TTAA 2 cut(s) 722, 1275
SatI GCNGC 5 cut(s) 91, 360, 526, 557, 783
ScaI AGTACT 1 cut(s) 983
ScrFI CCNGG 6 cut(s) 244, 344, 415, 522, 992, 1136
SduI GDGCHC 5 cut(s) 343, 450, 942, 1135, 1169
SfaNI GCATC 4 cut(s) 486, 605, 833, 1247
Sfr274I CTCGAG 1 cut(s) 1128
SfuI TTCGAA 1 cut(s) 570
SinI GGWCC 5 cut(s) 207, 856, 954, 972, 1177
SlaI CTCGAG 1 cut(s) 1128
SmiMI CAYNNNNRTG 2 cut(s) 915, 1020
SmlI CTYRAG 1 cut(s) 1128
SmoI CTYRAG 1 cut(s) 1128
Sse9I AATT 3 cut(s) 269, 639, 1276
SspI AATATT 1 cut(s) 1373
SspMI CTAG 3 cut(s) 96, 617, 1112
StyD4I CCNGG 6 cut(s) 242, 342, 413, 520, 990, 1134
StyI CCWWGG 2 cut(s) 1173, 1267
TaaI ACNGT 3 cut(s) 650, 1065, 1191
TaiI ACGT 4 cut(s) 56, 540, 951, 1043
TaqI TCGA 5 cut(s) 480, 570, 679, 807, 1129
TaqII GACCGA 1 cut(s) 942
TasI AATT 3 cut(s) 269, 639, 1276
TatI WGTACW 3 cut(s) 981, 1295, 1355
TauI GCSGC 3 cut(s) 362, 528, 559
TfiI GAWTC 2 cut(s) 110, 233
Tru1I TTAA 2 cut(s) 722, 1275
Tru9I TTAA 2 cut(s) 722, 1275
TscAI CASTG 1 cut(s) 1032
TseFI GTSAC 1 cut(s) 1075
TseI GCWGC 2 cut(s) 90, 782
Tsp45I GTSAC 1 cut(s) 1075
TspDTI ATGAA 1 cut(s) 822
TspGWI ACGGA 3 cut(s) 148, 295, 853
TspRI CASTG 1 cut(s) 1032
Van91I CCANNNNNTGG 1 cut(s) 634
VpaK11BI GGWCC 5 cut(s) 207, 856, 954, 972, 1177
XapI RAATTY 1 cut(s) 269
XbaI TCTAGA 1 cut(s) 616
XhoI CTCGAG 1 cut(s) 1128
XmiI GTMKAC 2 cut(s) 309, 1330
XspI CTAG 3 cut(s) 96, 617, 1112
ZrmI AGTACT 1 cut(s) 983
Zsp2I ATGCAT 1 cut(s) 839
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.