Rroxscaffold_1G00037380
ERF Family

Belongs to the protein kinase superfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0010356
Physical Location & Seq
Forward (+)
54505897 .. 54509952
4056 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00037380.1

Sequence Viewer

Length: 1371 bp
ATGGAGCCGTGGCTCGATGACTTAGCCGACGATCTCCAGAGCATTAGCTTCACCTCCACGGCCACAACCGCAGCCACGGAATCCAAACGAAGCACAAGCTCCGGCTCCGAAGCCACGCTAACCGCCTCGTGCTCCTCCTCGGCTCGCCTCGGCTCCCTCGTCGCCAAGCCCCGAGCCCCGTCCTCGGACCCCCGCTGGTCCGCAATCCACAAGATCCGATCCGAGTCCCCGACCCGGCAGCTGGCCCTCTCGGACCTCAAATTCTCCGAGCGGCTCGGCTCCGGCGACATCGGGTCGGTGTACCTGGCCGAGCTGAAGTCCGGCGACGGCAAGGGGTGCGTGTTCGCGGCGAAAGTGATGGACAAGAAGGAGCTGATTAGTCGGAGCAAAGAAGGGAGGGCGAGGACAGAGAGGGAAATACTGGAATTGTTGGAGCACCCATTTTTGCCCACGCTCTACGCCAGTATAGACGCTCCCAACTGGCTTTTCTTGCTCACCGAGTTCTGCCCCGGCGGTGACCTCCACGTACTCCGACAACGCCAGCCTTTGAAACGCTTCCATGAGTCTGCCGTCAGGTTCTATGCATCTGAGGTGGTTGTAGCTCTAGAGTACCTTCACATGATGGGAATTGTGTATCGTGATCTCAAGCCCGAAAACGTTTTAGTTCGATCCGATGGCCACATCATGCTTACAGATTTTGACCTCTCATTGAAATGCGATGATGTTGCATCTGCTGCTCATATAATTTCTGATCAAAACACTCCAGTCGCCGCCCCTCAAAAAGACTATGCAGTTGATCCACCTCGCTTTACCTCATCTTCATGCATCATTCCAAATTGTATAGTTCCTGCCGTGTCATGTTTCCACCCGAGGAGCAAGCGCAAGAAGAAGAAGCAGGGCCATCGGGACGGACCTGAGTTTGTGGCCGAGCCTGTTGACGTCCGGTCCATGTCTTTTGTTGGGACTCACGAATACTTGGCCCCTGAGATTGTGTCTGGCGAGGGGCATGGTAGTGCAGTGGATTGGTGGACATTAGGGATATTTATATTTGAACTCTTTTATGGCGTGACGCCCTTCAAGGGTGTGGACAATGAGCTAACCCTAGCTAATATTGTCGCTCGAGCCTTGGAGCTTCCGAAAGAACCTGCCGTGCCTTCTACGGCAAAGGACCTGATCTCACAGCTTTTGGCCAAGGACCCGGACAGGCGGTTGGGATCGTTAATGGGTGCATCAGCTATCAAACGACACCCATTTTTCCAAGGGGTCAATTGGGCACTGTTGAGATGTACTCCTCCACCATTTATTCCCCCACCATTTGCTAGACGCCTTGTATCTAATCAAACTTGTCCTGAGAAGATGGATTATTATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pkinase

Protein Analysis

456

Amino Acids

50.38

Weight (kDa)

6.87

Isoelectric Point (pI)

53.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PK_Tyr_Ser-Thr PF07714 86 - 240 3.8e-19 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 88 - 241 1.1e-32 Protein kinase domain
Pkinase PF00069 316 - 419 1.8e-20 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 324 - 403 2.6e-07 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 942
Acc36I ACCTGC 1 cut(s) 1153
AccBSI CCGCTC 1 cut(s) 271
AccII CGCG 1 cut(s) 347
AciI CCGC 9 cut(s) 69, 123, 193, 201, 271, 347, 513, 771, 1207
AclI AACGTT 1 cut(s) 657
AclWI GGATC 5 cut(s) 208, 213, 663, 791, 1222
AcoI YGGCCR 5 cut(s) 60, 306, 676, 924, 1188
AcsI RAATTY 1 cut(s) 260
AcuI CTGAAG 1 cut(s) 335
AcyI GRCGYC 3 cut(s) 939, 1070, 1324
AfaI GTAC 4 cut(s) 302, 528, 611, 1288
AfiI CCNNNNNNNGG 4 cut(s) 184, 234, 241, 1079
AgsI TTSAA 4 cut(s) 550, 712, 1052, 1078
AhdI GACNNNNNGTC 2 cut(s) 292, 943
AjnI CCWGG 1 cut(s) 303
AjuI GAANNNNNNNTTGG 2 cut(s) 470, 502
Alw21I GWGCWC 2 cut(s) 134, 438
AlwI GGATC 5 cut(s) 208, 213, 663, 791, 1222
Ama87I CYCGRG 3 cut(s) 171, 868, 1119
AoxI GGCC 8 cut(s) 60, 243, 306, 676, 898, 924, 978, 1188
ApeKI GCWGC 3 cut(s) 71, 238, 734
ApoI RAATTY 1 cut(s) 260
Asp700I GAANNNNTTC 1 cut(s) 554
AspLEI GCGC 1 cut(s) 882
AsuC2I CCSGG 3 cut(s) 235, 510, 1199
AsuHPI GGTGA 3 cut(s) 43, 487, 527
AvaI CYCGRG 3 cut(s) 171, 868, 1119
AvaII GGWCC 7 cut(s) 187, 198, 253, 911, 945, 1168, 1195
BaeGI GKGCMC 1 cut(s) 1276
BalI TGGCCA 2 cut(s) 678, 1190
BanII GRGCYC 1 cut(s) 178
BauI CACGAG 1 cut(s) 127
Bbv12I GWGCWC 2 cut(s) 134, 438
BbvI GCAGC 3 cut(s) 83, 250, 721
BccI CCATC 5 cut(s) 352, 616, 668, 909, 1351
BceAI ACGGC 6 cut(s) 75, 343, 554, 836, 1133, 1176
BcgI CGANNNNNNTGC 4 cut(s) 707, 741, 884, 918
BciT130I CCWGG 1 cut(s) 305
BclI TGATCA 1 cut(s) 751
BcnI CCSGG 3 cut(s) 235, 510, 1199
BfaI CTAG 3 cut(s) 605, 1103, 1320
BfuAI ACCTGC 1 cut(s) 1153
BisI GCNGC 6 cut(s) 72, 239, 272, 348, 735, 771
BlsI GCNGC 6 cut(s) 73, 240, 273, 349, 736, 772
Bme1390I CCNGG 4 cut(s) 235, 305, 510, 1199
Bme18I GGWCC 7 cut(s) 187, 198, 253, 911, 945, 1168, 1195
BmeRI GACNNNNNGTC 2 cut(s) 292, 943
BmeT110I CYCGRG 3 cut(s) 171, 868, 1119
BmiI GGNNCC 7 cut(s) 6, 106, 154, 189, 280, 981, 1197
BmrFI CCNGG 4 cut(s) 235, 305, 510, 1199
BmsI GCATC 4 cut(s) 593, 737, 834, 1238
BplI GAGNNNNNCTC 2 cut(s) 1273, 1305
BpmI CTGGAG 2 cut(s) 20, 747
BpuEI CTTGAG 1 cut(s) 629
BpuMI CCSGG 3 cut(s) 235, 510, 1199
BsaAI YACGTR 1 cut(s) 526
BsaHI GRCGYC 3 cut(s) 939, 1070, 1324
BsaWI WCCGGW 1 cut(s) 942
Bsc4I CCNNNNNNNGG 4 cut(s) 184, 234, 241, 1079
Bse1I ACTGG 4 cut(s) 426, 462, 485, 764
BseBI CCWGG 1 cut(s) 305
BseLI CCNNNNNNNGG 4 cut(s) 184, 234, 241, 1079
BseMII CTCAG 4 cut(s) 579, 906, 975, 1341
BseNI ACTGG 4 cut(s) 426, 462, 485, 764
BseRI GAGGAG 4 cut(s) 124, 127, 886, 1281
BseSI GKGCMC 1 cut(s) 1276
BseXI GCAGC 3 cut(s) 83, 250, 721
BsgI GTGCAG 1 cut(s) 1035
Bsh1236I CGCG 1 cut(s) 347
BshFI GGCC 8 cut(s) 62, 245, 308, 678, 900, 926, 980, 1190
BsiHKAI GWGCWC 2 cut(s) 134, 438
BsiHKCI CYCGRG 3 cut(s) 171, 868, 1119
BsiSI CCGG 7 cut(s) 102, 235, 282, 321, 510, 943, 1199
BslFI GGGAC 3 cut(s) 211, 920, 976
BslI CCNNNNNNNGG 4 cut(s) 184, 234, 241, 1079
BsmFI GGGAC 3 cut(s) 211, 920, 976
BsnI GGCC 8 cut(s) 62, 245, 308, 678, 900, 926, 980, 1190
BsoBI CYCGRG 3 cut(s) 171, 868, 1119
Bsp1286I GDGCHC 4 cut(s) 134, 178, 438, 1276
Bsp143I GATC 9 cut(s) 31, 213, 218, 640, 668, 751, 796, 1173, 1214
BspACI CCGC 9 cut(s) 69, 123, 193, 201, 271, 347, 513, 771, 1207
BspANI GGCC 8 cut(s) 62, 245, 308, 678, 900, 926, 980, 1190
BspCNI CTCAG 4 cut(s) 580, 907, 976, 1342
BspFNI CGCG 1 cut(s) 347
BspLI GGNNCC 7 cut(s) 6, 106, 154, 189, 280, 981, 1197
BspMI ACCTGC 1 cut(s) 1153
BspPI GGATC 5 cut(s) 208, 213, 663, 791, 1222
BsrBI CCGCTC 1 cut(s) 271
BsrI ACTGG 4 cut(s) 426, 462, 485, 764
BssMI GATC 9 cut(s) 31, 213, 218, 640, 668, 751, 796, 1173, 1214
BssNI GRCGYC 3 cut(s) 939, 1070, 1324
BssSI CACGAG 1 cut(s) 127
BssT1I CCWWGG 3 cut(s) 1125, 1191, 1258
Bst2BI CACGAG 1 cut(s) 127
Bst2UI CCWGG 1 cut(s) 305
Bst4CI ACNGT 1 cut(s) 1278
BstACI GRCGYC 3 cut(s) 939, 1070, 1324
BstAPI GCANNNNNTGC 2 cut(s) 336, 734
BstBAI YACGTR 1 cut(s) 526
BstC8I GCNNGC 4 cut(s) 145, 243, 542, 878
BstDEI CTNAG 5 cut(s) 22, 588, 915, 984, 1350
BstDSI CCRYGG 3 cut(s) 8, 57, 75
BstEII GGTNACC 1 cut(s) 515
BstFNI CGCG 1 cut(s) 347
BstHHI GCGC 1 cut(s) 882
BstKTI GATC 9 cut(s) 34, 216, 221, 643, 671, 754, 799, 1176, 1217
BstMBI GATC 9 cut(s) 31, 213, 218, 640, 668, 751, 796, 1173, 1214
BstMWI GCNNNNNNNGC 4 cut(s) 68, 336, 490, 734
BstNI CCWGG 1 cut(s) 305
BstPI GGTNACC 1 cut(s) 515
BstSCI CCNGG 4 cut(s) 233, 303, 508, 1197
BstSLI GKGCMC 1 cut(s) 1276
BstUI CGCG 1 cut(s) 347
BstV1I GCAGC 3 cut(s) 83, 250, 721
BstX2I RGATCY 1 cut(s) 213
BstYI RGATCY 1 cut(s) 213
BsuRI GGCC 8 cut(s) 62, 245, 308, 678, 900, 926, 980, 1190
BtgI CCRYGG 3 cut(s) 8, 57, 75
BtgZI GCGATG 1 cut(s) 732
BtsI GCAGTG 1 cut(s) 1023
BtsIMutI CAGTG 2 cut(s) 1023, 1274
BveI ACCTGC 1 cut(s) 1153
Cac8I GCNNGC 4 cut(s) 145, 243, 542, 878
CfoI GCGC 1 cut(s) 882
CseI GACGC 3 cut(s) 479, 1078, 1332
Csp6I GTAC 4 cut(s) 301, 527, 610, 1287
CviAII CATG 7 cut(s) 560, 619, 685, 822, 858, 949, 1007
CviQI GTAC 4 cut(s) 301, 527, 610, 1287
DdeI CTNAG 5 cut(s) 22, 588, 915, 984, 1350
DpnI GATC 9 cut(s) 33, 215, 220, 642, 670, 753, 798, 1175, 1216
DpnII GATC 9 cut(s) 31, 213, 218, 640, 668, 751, 796, 1173, 1214
DriI GACNNNNNGTC 2 cut(s) 292, 943
EaeI YGGCCR 5 cut(s) 60, 306, 676, 924, 1188
Eam1105I GACNNNNNGTC 2 cut(s) 292, 943
Eco130I CCWWGG 3 cut(s) 1125, 1191, 1258
Eco24I GRGCYC 1 cut(s) 178
Eco47I GGWCC 7 cut(s) 187, 198, 253, 911, 945, 1168, 1195
Eco57I CTGAAG 1 cut(s) 335
Eco88I CYCGRG 3 cut(s) 171, 868, 1119
Eco91I GGTNACC 1 cut(s) 515
EcoO109I RGGNCCY 2 cut(s) 1168, 1195
EcoO65I GGTNACC 1 cut(s) 515
EcoRII CCWGG 1 cut(s) 303
EcoT14I CCWWGG 3 cut(s) 1125, 1191, 1258
EcoT22I ATGCAT 2 cut(s) 586, 827
EcoT38I GRGCYC 1 cut(s) 178
ErhI CCWWGG 3 cut(s) 1125, 1191, 1258
FaeI CATG 7 cut(s) 563, 622, 688, 825, 861, 952, 1010
FaqI GGGAC 3 cut(s) 211, 920, 976
FatI CATG 7 cut(s) 559, 618, 684, 821, 857, 948, 1006
FauI CCCGC 1 cut(s) 200
FbaI TGATCA 1 cut(s) 751
Fnu4HI GCNGC 6 cut(s) 72, 239, 272, 348, 735, 771
FriOI GRGCYC 1 cut(s) 178
Fsp4HI GCNGC 6 cut(s) 72, 239, 272, 348, 735, 771
FspBI CTAG 3 cut(s) 605, 1103, 1320
GlaI GCGC 1 cut(s) 881
GluI GCNGC 6 cut(s) 72, 239, 272, 348, 735, 771
GsuI CTGGAG 2 cut(s) 20, 747
HaeIII GGCC 8 cut(s) 62, 245, 308, 678, 900, 926, 980, 1190
HapII CCGG 7 cut(s) 102, 235, 282, 321, 510, 943, 1199
HgaI GACGC 3 cut(s) 479, 1078, 1332
HhaI GCGC 1 cut(s) 882
Hin1I GRCGYC 3 cut(s) 939, 1070, 1324
Hin1II CATG 7 cut(s) 563, 622, 688, 825, 861, 952, 1010
Hin6I GCGC 1 cut(s) 880
HinP1I GCGC 1 cut(s) 880
HincII GTYRAC 1 cut(s) 937
HindII GTYRAC 1 cut(s) 937
HinfI GANTC 4 cut(s) 80, 224, 563, 964
HpaII CCGG 7 cut(s) 102, 235, 282, 321, 510, 943, 1199
HphI GGTGA 3 cut(s) 43, 487, 527
Hpy166II GTNNAC 4 cut(s) 301, 937, 1029, 1087
Hpy188III TCNNGA 6 cut(s) 37, 605, 638, 905, 968, 1349
Hpy8I GTNNAC 4 cut(s) 301, 937, 1029, 1087
Hpy99I CGWCG 3 cut(s) 32, 164, 329
HpyAV CCTTC 5 cut(s) 361, 386, 623, 1084, 1164
HpyCH4III ACNGT 1 cut(s) 1278
HpyCH4IV ACGT 3 cut(s) 525, 657, 939
HpyCH4V TGCA 6 cut(s) 584, 728, 791, 825, 1016, 1229
HpyF10VI GCNNNNNNNGC 4 cut(s) 68, 336, 490, 734
HpyF3I CTNAG 5 cut(s) 22, 588, 915, 984, 1350
HpySE526I ACGT 3 cut(s) 525, 657, 939
Hsp92I GRCGYC 3 cut(s) 939, 1070, 1324
Hsp92II CATG 7 cut(s) 563, 622, 688, 825, 861, 952, 1010
HspAI GCGC 1 cut(s) 880
Ksp22I TGATCA 1 cut(s) 751
Kzo9I GATC 9 cut(s) 31, 213, 218, 640, 668, 751, 796, 1173, 1214
Lsp1109I GCAGC 3 cut(s) 83, 250, 721
LweI GCATC 4 cut(s) 593, 737, 834, 1238
MaeI CTAG 3 cut(s) 605, 1103, 1320
MaeII ACGT 3 cut(s) 525, 657, 939
MaeIII GTNAC 2 cut(s) 515, 1066
MalI GATC 9 cut(s) 33, 215, 220, 642, 670, 753, 798, 1175, 1216
MbiI CCGCTC 1 cut(s) 271
MboI GATC 9 cut(s) 31, 213, 218, 640, 668, 751, 796, 1173, 1214
MboII GAAGA 4 cut(s) 810, 898, 901, 1366
MfeI CAATTG 1 cut(s) 1267
MflI RGATCY 1 cut(s) 213
MhlI GDGCHC 4 cut(s) 134, 178, 438, 1276
MlsI TGGCCA 2 cut(s) 678, 1190
MluCI AATT 6 cut(s) 260, 425, 627, 744, 835, 1267
MluNI TGGCCA 2 cut(s) 678, 1190
MlyI GAGTC 3 cut(s) 233, 572, 958
MmeI TCCRAC 3 cut(s) 362, 411, 556
Mox20I TGGCCA 2 cut(s) 678, 1190
Mph1103I ATGCAT 2 cut(s) 586, 827
MroXI GAANNNNTTC 1 cut(s) 554
MscI TGGCCA 2 cut(s) 678, 1190
MseI TTAA 1 cut(s) 1220
MslI CAYNNNNRTG 3 cut(s) 712, 820, 1011
Msp20I TGGCCA 2 cut(s) 678, 1190
MspA1I CMGCKG 2 cut(s) 195, 241
MspI CCGG 7 cut(s) 102, 235, 282, 321, 510, 943, 1199
MspR9I CCNGG 4 cut(s) 235, 305, 510, 1199
MunI CAATTG 1 cut(s) 1267
MvaI CCWGG 1 cut(s) 305
MvnI CGCG 1 cut(s) 347
MwoI GCNNNNNNNGC 4 cut(s) 68, 336, 490, 734
NciI CCSGG 3 cut(s) 235, 510, 1199
NdeII GATC 9 cut(s) 31, 213, 218, 640, 668, 751, 796, 1173, 1214
NlaIII CATG 7 cut(s) 563, 622, 688, 825, 861, 952, 1010
NlaIV GGNNCC 7 cut(s) 6, 106, 154, 189, 280, 981, 1197
NmeAIII GCCGAG 5 cut(s) 119, 129, 255, 334, 952
NmuCI GTSAC 2 cut(s) 515, 1066
NsiI ATGCAT 2 cut(s) 586, 827
PaeR7I CTCGAG 1 cut(s) 1119
PcsI WCGNNNNNNNCGW 2 cut(s) 156, 282
PdmI GAANNNNTTC 1 cut(s) 554
PfeI GAWTC 1 cut(s) 80
PkrI GCNGC 6 cut(s) 73, 240, 273, 349, 736, 772
PleI GAGTC 3 cut(s) 232, 571, 958
PpsI GAGTC 3 cut(s) 232, 571, 958
Ppu21I YACGTR 1 cut(s) 526
PpuMI RGGWCCY 2 cut(s) 1168, 1195
Psp1406I AACGTT 1 cut(s) 657
Psp5II RGGWCCY 2 cut(s) 1168, 1195
Psp6I CCWGG 1 cut(s) 303
PspEI GGTNACC 1 cut(s) 515
PspGI CCWGG 1 cut(s) 303
PspN4I GGNNCC 7 cut(s) 6, 106, 154, 189, 280, 981, 1197
PspPPI RGGWCCY 2 cut(s) 1168, 1195
PspXI VCTCGAGB 1 cut(s) 1119
PsuI RGATCY 1 cut(s) 213
PvuII CAGCTG 1 cut(s) 241
RsaI GTAC 4 cut(s) 302, 528, 611, 1288
RsaNI GTAC 4 cut(s) 301, 527, 610, 1287
RseI CAYNNNNRTG 3 cut(s) 712, 820, 1011
SaqAI TTAA 1 cut(s) 1220
SatI GCNGC 6 cut(s) 72, 239, 272, 348, 735, 771
Sau3AI GATC 9 cut(s) 31, 213, 218, 640, 668, 751, 796, 1173, 1214
SchI GAGTC 3 cut(s) 233, 572, 958
ScrFI CCNGG 4 cut(s) 235, 305, 510, 1199
SduI GDGCHC 4 cut(s) 134, 178, 438, 1276
SfaNI GCATC 4 cut(s) 593, 737, 834, 1238
Sfr274I CTCGAG 1 cut(s) 1119
SinI GGWCC 7 cut(s) 187, 198, 253, 911, 945, 1168, 1195
SlaI CTCGAG 1 cut(s) 1119
SmiMI CAYNNNNRTG 3 cut(s) 712, 820, 1011
SmlI CTYRAG 2 cut(s) 644, 1119
SmoI CTYRAG 2 cut(s) 644, 1119
Sse9I AATT 6 cut(s) 260, 425, 627, 744, 835, 1267
SsiI CCGC 9 cut(s) 69, 123, 193, 201, 271, 347, 513, 771, 1207
SspI AATATT 1 cut(s) 1111
SspMI CTAG 3 cut(s) 605, 1103, 1320
StyD4I CCNGG 4 cut(s) 233, 303, 508, 1197
StyI CCWWGG 3 cut(s) 1125, 1191, 1258
TaaI ACNGT 1 cut(s) 1278
TaiI ACGT 3 cut(s) 528, 660, 942
TaqI TCGA 3 cut(s) 15, 667, 1120
TasI AATT 6 cut(s) 260, 425, 627, 744, 835, 1267
TatI WGTACW 1 cut(s) 1286
TauI GCSGC 3 cut(s) 274, 350, 773
TfiI GAWTC 1 cut(s) 80
Tru1I TTAA 1 cut(s) 1220
Tru9I TTAA 1 cut(s) 1220
TscAI CASTG 2 cut(s) 1023, 1281
TseFI GTSAC 2 cut(s) 515, 1066
TseI GCWGC 3 cut(s) 71, 238, 734
Tsp45I GTSAC 2 cut(s) 515, 1066
TspDTI ATGAA 1 cut(s) 810
TspGWI ACGGA 2 cut(s) 92, 924
TspRI CASTG 2 cut(s) 1023, 1281
VpaK11BI GGWCC 7 cut(s) 187, 198, 253, 911, 945, 1168, 1195
XapI RAATTY 1 cut(s) 260
XbaI TCTAGA 1 cut(s) 604
XhoI CTCGAG 1 cut(s) 1119
XmnI GAANNNNTTC 1 cut(s) 554
XspI CTAG 3 cut(s) 605, 1103, 1320
ZraI GACGTC 1 cut(s) 940
Zsp2I ATGCAT 2 cut(s) 586, 827
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.