MD11G1160000.v1.1
ERF Family

Belongs to the protein kinase superfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0010356
Physical Location & Seq
Forward (+)
15680285 .. 15684738
4454 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1160000.v1.1.491

Sequence Viewer

Length: 1380 bp
ATGGAGCCCTGTCTGGACGACTTAGCCGACGACCTCCAAAGCCTGAGCTTCACCTCCACCACAACCACCAACACCACCACCACCAAGCGCAGCACCAGCTCCTGCTCCGAATCCACGCTCCCGCCCTCCGCCTCCTCCTCTGCTCGCCTCCCCGCCTCCGTAGTCCCCAAGTCCCGACCCCAATCCTCTTCCTCCGACCCTCTCTGGTCAGCAATCTACAAGATCCGGTCCGAATCCCCGACCCGGCAACTCTCCCTTTCTGACCTCAAATTCGCGGAGCGCCTCGGCTCCGGCGACATCGGCTCCGTCTACTTGGCCGAGTTGAAGTCCCCCGGTGGGGTGCAGGGGTGCATATTTGCGGCGAAGGTGATGGACAAGAAGGAATTGATTAGCCGGAGAAAGGAAGGGCGGGCGAGGACAGAGAGGGAAATATTGGAAATGTTGGAGCACCCGTTCTTGCCCACGCTCTACGCGAATATCGACGCGCCCAACTGGTTTTTCCTGCTGACAGAGTTCTGCCCCGGCGGCGACCTCCACGTCCTCCGCCAGCGCCAGCCGCTCAAACGCTTCGACGAACCTGCCGTCAGGTTCTACGCATCGGAGGTGATTGTAGCTCTTGAGTACCTCCACATGATGGGGATTGTGTACCGTGATCTGAAGCCGGAAAACGTTTTAGTTCGATCTGACGGTCACATCATGCTTACGGATTTTGACCTCTCATTAAAATGCGACGATGGTACATCAACGCCTCAGATCATCTCTACTCAAAATACCCCAAATGCTGCACCACAAAAAGACTACTCACTTGATCCACCCCCATTTGCATCATCTTCATGCATACTTCCCAGTTGTATGGTCCCTTCCGTGTCATGTTTTCACCCGAAAAACAAGCGGAAGAAGAAGCAGAGCCATAAAAATGGCCCCGAGTTTGTGTCCGAGCCCGTTGACGTCCGGTCCATGTCGTTTGTCGGGACACACGAGTACTTGGCTCCGGAGATTGTGTCCGGTGAGGGACATGGTAGTGCAGTGGACTGGTGGACGTTGGGGATATTTATTTTTGAACTATTCTATGGTGTGACACCCTTTAGGGGCGTGGACAATGAGCTAACCCTAGCAAATATTGTGGCTCGAGCCCTAGAGCTTCCGAAAGAACCGGCTGTGCCGGCCACAGCCAAGGACCTCATCTCACAACTGCTGGTCAAGGATCCGGCTCGGCGATTGGGATCGTTGATGGGTGCATCGGCAGTCAAGAGGCACCCATTTTTCCAAGGGGTTAATTGGGCGTTGTTGAGATGTACACCTCCACCATTTATTCCCCCACCATTTAGTAGACGAGTTGTATCTGATCAAAGTTGTTCTGAGAAAATCGAGTATTATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pkinase

Protein Analysis

460

Amino Acids

50.83

Weight (kDa)

6.77

Isoelectric Point (pI)

65.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PK_Tyr_Ser-Thr PF07714 89 - 244 2.8e-18 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 91 - 256 2.5e-32 Protein kinase domain
Pkinase PF00069 319 - 422 9.3e-20 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 327 - 406 1.9e-06 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 536
AatII GACGTC 1 cut(s) 951
Acc36I ACCTGC 1 cut(s) 586
AccB1I GGYRCC 1 cut(s) 1254
AccB7I CCANNNNNTGG 1 cut(s) 634
AccBSI CCGCTC 1 cut(s) 559
AccI GTMKAC 2 cut(s) 309, 1330
AccII CGCG 3 cut(s) 275, 473, 485
AccIII TCCGGA 1 cut(s) 991
AclI AACGTT 1 cut(s) 669
AclWI GGATC 5 cut(s) 217, 803, 1199, 1212, 1231
AcoI YGGCCR 2 cut(s) 315, 1164
AcsI RAATTY 1 cut(s) 269
AcuI CTGAAG 1 cut(s) 677
AcyI GRCGYC 1 cut(s) 948
AfaI GTAC 5 cut(s) 623, 647, 739, 983, 1297
AfiI CCNNNNNNNGG 6 cut(s) 243, 336, 337, 400, 634, 1088
AgsI TTSAA 2 cut(s) 325, 1061
AhdI GACNNNNNGTC 1 cut(s) 952
AjiI CACGTC 1 cut(s) 538
AjuI GAANNNNNNNTTGG 2 cut(s) 482, 514
AloI GAACNNNNNNTCC 2 cut(s) 437, 469
AluBI AGCT 5 cut(s) 48, 99, 614, 1105, 1141
AluI AGCT 5 cut(s) 48, 99, 614, 1105, 1141
Alw21I GWGCWC 1 cut(s) 450
AlwI GGATC 5 cut(s) 217, 803, 1199, 1212, 1231
AlwNI CAGNNNCTG 1 cut(s) 102
Ama87I CYCGRG 2 cut(s) 923, 1128
Aor13HI TCCGGA 1 cut(s) 991
AoxI GGCC 3 cut(s) 315, 919, 1164
ApeKI GCWGC 2 cut(s) 90, 782
ApoI RAATTY 1 cut(s) 269
ArsI GACNNNNNNTTYG 4 cut(s) 254, 286, 567, 599
AspLEI GCGC 4 cut(s) 90, 282, 487, 552
AspS9I GGNCC 5 cut(s) 228, 856, 920, 954, 1177
AsuC2I CCSGG 3 cut(s) 244, 333, 522
AsuHPI GGTGA 5 cut(s) 43, 379, 616, 869, 1019
AvaI CYCGRG 2 cut(s) 923, 1128
AvaII GGWCC 4 cut(s) 228, 856, 954, 1177
BamHI GGATCC 1 cut(s) 1204
BanI GGYRCC 1 cut(s) 1254
BanII GRGCYC 3 cut(s) 9, 942, 1135
BarI GAAGNNNNNNTAC 2 cut(s) 844, 876
BauI CACGAG 1 cut(s) 977
Bbv12I GWGCWC 1 cut(s) 450
BbvI GCAGC 2 cut(s) 102, 769
BccI CCATC 4 cut(s) 364, 628, 728, 1225
BceAI ACGGC 1 cut(s) 566
BcgI CGANNNNNNTGC 2 cut(s) 560, 594
BclI TGATCA 1 cut(s) 1345
BcnI CCSGG 3 cut(s) 244, 333, 522
BfaI CTAG 2 cut(s) 1112, 1136
BfoI RGCGCY 2 cut(s) 283, 553
BfuAI ACCTGC 1 cut(s) 586
BglI GCCNNNNNGGC 1 cut(s) 525
BisI GCNGC 5 cut(s) 91, 360, 526, 557, 783
BlsI GCNGC 5 cut(s) 92, 361, 527, 558, 784
BmcAI AGTACT 1 cut(s) 983
Bme1390I CCNGG 3 cut(s) 244, 333, 522
Bme18I GGWCC 4 cut(s) 228, 856, 954, 1177
BmeRI GACNNNNNGTC 1 cut(s) 952
BmeT110I CYCGRG 2 cut(s) 923, 1128
BmgBI CACGTC 1 cut(s) 538
BmgT120I GGNCC 5 cut(s) 228, 856, 920, 954, 1177
BmiI GGNNCC 8 cut(s) 6, 289, 304, 858, 922, 990, 1206, 1256
BmrFI CCNGG 3 cut(s) 244, 333, 522
BmrI ACTGGG 1 cut(s) 840
BmsI GCATC 3 cut(s) 605, 833, 1247
BmuI ACTGGG 1 cut(s) 840
Bpu10I CCTNAGC 1 cut(s) 44
BpuEI CTTGAG 1 cut(s) 638
BpuMI CCSGG 3 cut(s) 244, 333, 522
BsaBI GATNNNNATC 1 cut(s) 1222
BsaHI GRCGYC 1 cut(s) 948
BsaJI CCNNGG 5 cut(s) 283, 331, 520, 1173, 1267
BsaWI WCCGGW 4 cut(s) 225, 951, 991, 1004
BsaXI ACNNNNNCTCC 6 cut(s) 287, 317, 437, 467, 986, 1016
Bsc4I CCNNNNNNNGG 6 cut(s) 243, 336, 337, 400, 634, 1088
Bse118I RCCGGY 2 cut(s) 1153, 1162
Bse1I ACTGG 3 cut(s) 497, 846, 1037
Bse8I GATNNNNATC 1 cut(s) 1222
BseAI TCCGGA 1 cut(s) 991
BseDI CCNNGG 5 cut(s) 283, 331, 520, 1173, 1267
BseJI GATNNNNATC 1 cut(s) 1222
BseLI CCNNNNNNNGG 6 cut(s) 243, 336, 337, 400, 634, 1088
BseMII CTCAG 3 cut(s) 35, 764, 1350
BseNI ACTGG 3 cut(s) 497, 846, 1037
BseRI GAGGAG 2 cut(s) 124, 127
BseXI GCAGC 2 cut(s) 102, 769
BsgI GTGCAG 3 cut(s) 362, 768, 1044
Bsh1236I CGCG 3 cut(s) 275, 473, 485
BshFI GGCC 3 cut(s) 317, 921, 1166
BshNI GGYRCC 1 cut(s) 1254
BsiHKAI GWGCWC 1 cut(s) 450
BsiHKCI CYCGRG 2 cut(s) 923, 1128
BslFI GGGAC 6 cut(s) 149, 157, 313, 842, 985, 1026
BslI CCNNNNNNNGG 6 cut(s) 243, 336, 337, 400, 634, 1088
BsmFI GGGAC 6 cut(s) 149, 157, 313, 842, 985, 1026
BsnI GGCC 3 cut(s) 317, 921, 1166
BsoBI CYCGRG 2 cut(s) 923, 1128
Bsp1286I GDGCHC 4 cut(s) 9, 450, 942, 1135
Bsp13I TCCGGA 1 cut(s) 991
Bsp1407I TGTACA 1 cut(s) 1295
Bsp143I GATC 8 cut(s) 222, 652, 680, 753, 808, 1204, 1223, 1345
BspANI GGCC 3 cut(s) 317, 921, 1166
BspCNI CTCAG 3 cut(s) 36, 763, 1351
BspEI TCCGGA 1 cut(s) 991
BspFNI CGCG 3 cut(s) 275, 473, 485
BspLI GGNNCC 8 cut(s) 6, 289, 304, 858, 922, 990, 1206, 1256
BspMI ACCTGC 1 cut(s) 586
BspPI GGATC 5 cut(s) 217, 803, 1199, 1212, 1231
BspT107I GGYRCC 1 cut(s) 1254
BsrBI CCGCTC 1 cut(s) 559
BsrFI RCCGGY 2 cut(s) 1153, 1162
BsrGI TGTACA 1 cut(s) 1295
BsrI ACTGG 3 cut(s) 497, 846, 1037
BssAI RCCGGY 2 cut(s) 1153, 1162
BssECI CCNNGG 5 cut(s) 283, 331, 520, 1173, 1267
BssMI GATC 8 cut(s) 222, 652, 680, 753, 808, 1204, 1223, 1345
BssNI GRCGYC 1 cut(s) 948
BssSI CACGAG 1 cut(s) 977
BssT1I CCWWGG 2 cut(s) 1173, 1267
Bst2BI CACGAG 1 cut(s) 977
Bst4CI ACNGT 2 cut(s) 650, 689
Bst6I CTCTTC 1 cut(s) 193
BstACI GRCGYC 1 cut(s) 948
BstAUI TGTACA 1 cut(s) 1295
BstC8I GCNNGC 5 cut(s) 145, 411, 548, 554, 1164
BstDEI CTNAG 4 cut(s) 22, 44, 750, 1359
BstFNI CGCG 3 cut(s) 275, 473, 485
BstH2I RGCGCY 2 cut(s) 283, 553
BstHHI GCGC 4 cut(s) 90, 282, 487, 552
BstKTI GATC 8 cut(s) 225, 655, 683, 756, 811, 1207, 1226, 1348
BstMBI GATC 8 cut(s) 222, 652, 680, 753, 808, 1204, 1223, 1345
BstMWI GCNNNNNNNGC 5 cut(s) 96, 300, 525, 556, 1163
BstSCI CCNGG 3 cut(s) 242, 331, 520
BstUI CGCG 3 cut(s) 275, 473, 485
BstV1I GCAGC 2 cut(s) 102, 769
BstX2I RGATCY 2 cut(s) 222, 1204
BstXI CCANNNNNNTGG 2 cut(s) 853, 917
BstYI RGATCY 2 cut(s) 222, 1204
BsuRI GGCC 3 cut(s) 317, 921, 1166
BtrI CACGTC 1 cut(s) 538
BtsI GCAGTG 1 cut(s) 1032
BtsIMutI CAGTG 1 cut(s) 1032
BveI ACCTGC 1 cut(s) 586
Cac8I GCNNGC 5 cut(s) 145, 411, 548, 554, 1164
CaiI CAGNNNCTG 1 cut(s) 102
CfoI GCGC 4 cut(s) 90, 282, 487, 552
Cfr10I RCCGGY 2 cut(s) 1153, 1162
Cfr13I GGNCC 5 cut(s) 228, 856, 920, 954, 1177
CpoI CGGWCCG 1 cut(s) 228
CseI GACGC 1 cut(s) 491
Csp6I GTAC 5 cut(s) 622, 646, 738, 982, 1296
CspCI CAANNNNNGTGG 2 cut(s) 1104, 1139
CspI CGGWCCG 1 cut(s) 228
CviAII CATG 6 cut(s) 631, 697, 834, 870, 958, 1016
CviQI GTAC 5 cut(s) 622, 646, 738, 982, 1296
DdeI CTNAG 4 cut(s) 22, 44, 750, 1359
DpnI GATC 8 cut(s) 224, 654, 682, 755, 810, 1206, 1225, 1347
DpnII GATC 8 cut(s) 222, 652, 680, 753, 808, 1204, 1223, 1345
DrdI GACNNNNNNGTC 1 cut(s) 536
DriI GACNNNNNGTC 1 cut(s) 952
DseDI GACNNNNNNGTC 1 cut(s) 536
EaeI YGGCCR 2 cut(s) 315, 1164
Eam1104I CTCTTC 1 cut(s) 193
Eam1105I GACNNNNNGTC 1 cut(s) 952
EarI CTCTTC 1 cut(s) 193
EciI GGCGGA 2 cut(s) 118, 533
Eco130I CCWWGG 2 cut(s) 1173, 1267
Eco24I GRGCYC 3 cut(s) 9, 942, 1135
Eco47I GGWCC 4 cut(s) 228, 856, 954, 1177
Eco57I CTGAAG 1 cut(s) 677
Eco88I CYCGRG 2 cut(s) 923, 1128
EcoO109I RGGNCCY 1 cut(s) 1177
EcoT14I CCWWGG 2 cut(s) 1173, 1267
EcoT22I ATGCAT 1 cut(s) 839
EcoT38I GRGCYC 3 cut(s) 9, 942, 1135
ErhI CCWWGG 2 cut(s) 1173, 1267
FaeI CATG 6 cut(s) 634, 700, 837, 873, 961, 1019
FaqI GGGAC 6 cut(s) 149, 157, 313, 842, 985, 1026
FatI CATG 6 cut(s) 630, 696, 833, 869, 957, 1015
FauI CCCGC 3 cut(s) 129, 160, 402
FbaI TGATCA 1 cut(s) 1345
FblI GTMKAC 2 cut(s) 309, 1330
Fnu4HI GCNGC 5 cut(s) 91, 360, 526, 557, 783
FriOI GRGCYC 3 cut(s) 9, 942, 1135
Fsp4HI GCNGC 5 cut(s) 91, 360, 526, 557, 783
FspBI CTAG 2 cut(s) 1112, 1136
GlaI GCGC 4 cut(s) 89, 281, 486, 551
GluI GCNGC 5 cut(s) 91, 360, 526, 557, 783
HaeII RGCGCY 2 cut(s) 283, 553
HaeIII GGCC 3 cut(s) 317, 921, 1166
HgaI GACGC 1 cut(s) 491
HhaI GCGC 4 cut(s) 90, 282, 487, 552
Hin1I GRCGYC 1 cut(s) 948
Hin1II CATG 6 cut(s) 634, 700, 837, 873, 961, 1019
Hin6I GCGC 4 cut(s) 88, 280, 485, 550
HinP1I GCGC 4 cut(s) 88, 280, 485, 550
HincII GTYRAC 1 cut(s) 946
HindII GTYRAC 1 cut(s) 946
HinfI GANTC 2 cut(s) 110, 233
HphI GGTGA 5 cut(s) 43, 379, 616, 869, 1019
Hpy166II GTNNAC 8 cut(s) 310, 646, 946, 1030, 1038, 1096, 1298, 1331
Hpy188III TCNNGA 6 cut(s) 14, 174, 617, 970, 992, 1249
Hpy8I GTNNAC 8 cut(s) 310, 646, 946, 1030, 1038, 1096, 1298, 1331
Hpy99I CGWCG 4 cut(s) 32, 485, 575, 734
HpyAV CCTTC 4 cut(s) 358, 373, 398, 870
HpyCH4III ACNGT 2 cut(s) 650, 689
HpyCH4IV ACGT 4 cut(s) 537, 669, 948, 1040
HpyCH4V TGCA 7 cut(s) 343, 351, 785, 824, 837, 1025, 1238
HpyF10VI GCNNNNNNNGC 5 cut(s) 96, 300, 525, 556, 1163
HpyF3I CTNAG 4 cut(s) 22, 44, 750, 1359
HpySE526I ACGT 4 cut(s) 537, 669, 948, 1040
Hsp92I GRCGYC 1 cut(s) 948
Hsp92II CATG 6 cut(s) 634, 700, 837, 873, 961, 1019
HspAI GCGC 4 cut(s) 88, 280, 485, 550
Kpn2I TCCGGA 1 cut(s) 991
KroI GCCGGC 1 cut(s) 1162
KroNI GCCGGC 1 cut(s) 1164
Ksp22I TGATCA 1 cut(s) 1345
Kzo9I GATC 8 cut(s) 222, 652, 680, 753, 808, 1204, 1223, 1345
LmnI GCTCC 9 cut(s) 4, 104, 110, 123, 277, 293, 308, 445, 994
Lsp1109I GCAGC 2 cut(s) 102, 769
LweI GCATC 3 cut(s) 605, 833, 1247
MaeI CTAG 2 cut(s) 1112, 1136
MaeII ACGT 4 cut(s) 537, 669, 948, 1040
MaeIII GTNAC 2 cut(s) 689, 1075
MalI GATC 8 cut(s) 224, 654, 682, 755, 810, 1206, 1225, 1347
MbiI CCGCTC 1 cut(s) 559
MboI GATC 8 cut(s) 222, 652, 680, 753, 808, 1204, 1223, 1345
MboII GAAGA 4 cut(s) 180, 822, 907, 910
MflI RGATCY 2 cut(s) 222, 1204
MhlI GDGCHC 4 cut(s) 9, 450, 942, 1135
MluCI AATT 3 cut(s) 269, 383, 1276
MmeI TCCRAC 2 cut(s) 219, 423
Mph1103I ATGCAT 1 cut(s) 839
MroI TCCGGA 1 cut(s) 991
MroNI GCCGGC 1 cut(s) 1162
MseI TTAA 2 cut(s) 722, 1275
MslI CAYNNNNRTG 4 cut(s) 724, 832, 915, 1020
MspR9I CCNGG 3 cut(s) 244, 333, 522
MvnI CGCG 3 cut(s) 275, 473, 485
MwoI GCNNNNNNNGC 5 cut(s) 96, 300, 525, 556, 1163
NaeI GCCGGC 1 cut(s) 1164
NciI CCSGG 3 cut(s) 244, 333, 522
NdeII GATC 8 cut(s) 222, 652, 680, 753, 808, 1204, 1223, 1345
NgoMIV GCCGGC 1 cut(s) 1162
NlaIII CATG 6 cut(s) 634, 700, 837, 873, 961, 1019
NlaIV GGNNCC 8 cut(s) 6, 289, 304, 858, 922, 990, 1206, 1256
NmeAIII GCCGAG 3 cut(s) 264, 343, 1192
NmuCI GTSAC 2 cut(s) 689, 1075
NsiI ATGCAT 1 cut(s) 839
PaeR7I CTCGAG 1 cut(s) 1128
PcsI WCGNNNNNNNCGW 6 cut(s) 24, 291, 470, 477, 579, 975
PdiI GCCGGC 1 cut(s) 1164
PfeI GAWTC 2 cut(s) 110, 233
PflMI CCANNNNNTGG 1 cut(s) 634
PkrI GCNGC 5 cut(s) 92, 361, 527, 558, 784
PpuMI RGGWCCY 1 cut(s) 1177
Psp1406I AACGTT 1 cut(s) 669
Psp5II RGGWCCY 1 cut(s) 1177
PspN4I GGNNCC 8 cut(s) 6, 289, 304, 858, 922, 990, 1206, 1256
PspPI GGNCC 5 cut(s) 228, 856, 920, 954, 1177
PspPPI RGGWCCY 1 cut(s) 1177
PspXI VCTCGAGB 1 cut(s) 1128
PstNI CAGNNNCTG 1 cut(s) 102
PsuI RGATCY 2 cut(s) 222, 1204
RsaI GTAC 5 cut(s) 623, 647, 739, 983, 1297
RsaNI GTAC 5 cut(s) 622, 646, 738, 982, 1296
RseI CAYNNNNRTG 4 cut(s) 724, 832, 915, 1020
Rsr2I CGGWCCG 1 cut(s) 228
RsrII CGGWCCG 1 cut(s) 228
SaqAI TTAA 2 cut(s) 722, 1275
SatI GCNGC 5 cut(s) 91, 360, 526, 557, 783
Sau3AI GATC 8 cut(s) 222, 652, 680, 753, 808, 1204, 1223, 1345
Sau96I GGNCC 5 cut(s) 228, 856, 920, 954, 1177
ScaI AGTACT 1 cut(s) 983
ScrFI CCNGG 3 cut(s) 244, 333, 522
SduI GDGCHC 4 cut(s) 9, 450, 942, 1135
SfaNI GCATC 3 cut(s) 605, 833, 1247
Sfr274I CTCGAG 1 cut(s) 1128
SinI GGWCC 4 cut(s) 228, 856, 954, 1177
SlaI CTCGAG 1 cut(s) 1128
SmiMI CAYNNNNRTG 4 cut(s) 724, 832, 915, 1020
SmlI CTYRAG 2 cut(s) 617, 1128
SmoI CTYRAG 2 cut(s) 617, 1128
Sse9I AATT 3 cut(s) 269, 383, 1276
SspI AATATT 2 cut(s) 432, 1120
SspMI CTAG 2 cut(s) 1112, 1136
StyD4I CCNGG 3 cut(s) 242, 331, 520
StyI CCWWGG 2 cut(s) 1173, 1267
TaaI ACNGT 2 cut(s) 650, 689
TaiI ACGT 4 cut(s) 540, 672, 951, 1043
TaqI TCGA 5 cut(s) 480, 570, 679, 1129, 1368
TasI AATT 3 cut(s) 269, 383, 1276
TatI WGTACW 2 cut(s) 981, 1295
TauI GCSGC 3 cut(s) 362, 528, 559
TfiI GAWTC 2 cut(s) 110, 233
Tru1I TTAA 2 cut(s) 722, 1275
Tru9I TTAA 2 cut(s) 722, 1275
TscAI CASTG 1 cut(s) 1032
TseFI GTSAC 2 cut(s) 689, 1075
TseI GCWGC 2 cut(s) 90, 782
Tsp45I GTSAC 2 cut(s) 689, 1075
TspDTI ATGAA 1 cut(s) 822
TspGWI ACGGA 4 cut(s) 148, 295, 719, 853
TspRI CASTG 1 cut(s) 1032
Van91I CCANNNNNTGG 1 cut(s) 634
VpaK11BI GGWCC 4 cut(s) 228, 856, 954, 1177
XapI RAATTY 1 cut(s) 269
XhoI CTCGAG 1 cut(s) 1128
XmiI GTMKAC 2 cut(s) 309, 1330
XspI CTAG 2 cut(s) 1112, 1136
ZraI GACGTC 1 cut(s) 949
ZrmI AGTACT 1 cut(s) 983
Zsp2I ATGCAT 1 cut(s) 839
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.