MD03G1146100.v1.1

Ring finger

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0010718
Physical Location & Seq
Forward (+)
16016550 .. 16020735
4186 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1146100.v1.1.491

Sequence Viewer

Length: 1314 bp
ATGGGATCAGCTTGCTGTGTTGCTGCAAAGAAAAGAACCCACCCAAATCAAAGTGAAGGTGAAGCATTGCACAGGAATGCCATTCATTCACCATCATGGAGTTTTCAGTGGGATAATCGTCGGCGTGTCGCTGGTGAAATTGATACTCCATATCAAGTCTCTGGTGGAATCAGTACTAATGATAGCATGGAGTTAAAAGGTGCATTAAGTTCTGAAAGGGGTAATTTCTCAGATCTGGGAAGCCCGCTGGAGAATTTTGGAACACCTATATCTCAAAAGTCCCCTGTTCATGGAGGAACAGGTAAAAATCTGAGGAGTCCATCTCCAGATCTAACCATTGCAAGAAATTATTCTCCAGACGTGAAGAGTTTGACAGAATCACCAGGAATTGCAAATTCAGCCGTGTCAAATCTTTTGTTGTCCATGCCTACAACTTTTTCAACACCAACTGCAGAAACATTACGTACCCCTAGCTACCCTCTATTTCCCAACTCAACCCCATCAAGACGAGCTCGTCGTTCACCAGGACATCAGCTGTTGAGACAGGTTTCTGATAGTCGGATATTGGGATTAAAATCTCCAAATAACAACTCAATCTCTGAAGGAAGGCAATCATTTGTACTCTCCACTTGCAGCAATGACTTGGCAACTGGATCTCTAGGCGGGTCTTCTGATGGCTGGTCAATGCGTACCTTTTCTGAGCTCGTGGCGTATTCTCAAAGGGAAAGATTGTCTTTTGACAGTGAGCACTTTGGTTCTATCCATGGAAAGCTAAGTGGATCAAGCAGCAGGTTCTCGTGTTCTCCTTCCATAGATTTGCAGTCTTGTGGTGTCTGCTCAAAGCTCTTAACGGAGAGATCTTCATGGAGCGCCCAGAAAATTATTGCTGGCAGCGACACTTCAGTGGTTTCTGTGCTGGTATGTGGACATATTTACCATGCTGAGTGCTTGGAGACTATGACCTTGGAGGTTGATAGGTATGACCCGGCTTGCCCAATTTGTATGGTCGGAGAGAAGCAAGTCTTAAAGATGTCGAGAAAAGCTTTGAGGGCAGAAGCAGAGCTCAAAGCCAAAAACCACAAGGTATCCAAAAATAGAGTTGTAGATAGCTACTTTGATAGTGATAACGATGTCTTTGATCAGCAGAAGAATGCCAATAGAAAAGGACAGGTTTCTAAGATGGAACCGAGTTCCAGCACTCGGAATTCCTCTGCGAAGCCTTTCTTGAAACGACACTTCTCGCTTGGATCAAGGTGGAGTAGATCCCTATCGGAAAACGATTCTGCCAGGAGGAAGGGGTTTTGGGCCAGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-C3HC4

Protein Analysis

438

Amino Acids

47.77

Weight (kDa)

9.45

Isoelectric Point (pI)

65.56

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010718)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G19680 AT1G75400
fragaria_vesca FvH4_7g07801 FvH4_7g07801 FvH4_7g07801
malus_domestica MD03G1146100.v1.1
pyrus_communis pycom03g10050
rosa_chinensis RchiOBHm_Chr5g0045191
rosa_laevigata RLG00000034342
rosa_multiflora Rmu_co8061458.1_g000001 Rmu_sc0001773.1_g000013
rosa_roxburghii Rroxscaffold_1G00035840
rosa_rugosa Rorug05G0221500.1 Rorug05G0221600
rosa_samantha Rh5AG302900 Rh5BG311400 Rh5CG337400 Rh5DG321600
rosa_wichuraiana Rw5G028110 Rw5G028470

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 513
Acc36I ACCTGC 1 cut(s) 780
AciI CCGC 2 cut(s) 245, 663
AclWI GGATC 5 cut(s) 13, 661, 787, 1255, 1257
AcsI RAATTY 3 cut(s) 253, 394, 1204
AcuI CTGAAG 2 cut(s) 621, 885
AfaI GTAC 4 cut(s) 175, 466, 621, 691
AfiI CCNNNNNNNGG 2 cut(s) 290, 1200
AgsI TTSAA 2 cut(s) 441, 1228
AjiI CACGTC 1 cut(s) 361
AjnI CCWGG 3 cut(s) 382, 523, 1286
AleI CACNNNNGTG 1 cut(s) 902
Alw21I GWGCWC 4 cut(s) 514, 705, 750, 1065
Alw26I GTCTC 3 cut(s) 163, 535, 947
AlwI GGATC 5 cut(s) 13, 661, 787, 1255, 1257
AoxI GGCC 1 cut(s) 1305
ApeKI GCWGC 4 cut(s) 23, 633, 786, 891
ApoI RAATTY 3 cut(s) 253, 394, 1204
Asp700I GAANNNNTTC 2 cut(s) 349, 1220
AspLEI GCGC 1 cut(s) 872
AspS9I GGNCC 1 cut(s) 1305
AsuC2I CCSGG 1 cut(s) 986
AsuHPI GGTGA 5 cut(s) 71, 81, 146, 372, 513
BanII GRGCYC 3 cut(s) 514, 705, 1065
BauI CACGAG 2 cut(s) 704, 796
BbsI GAAGAC 1 cut(s) 660
Bbv12I GWGCWC 4 cut(s) 514, 705, 750, 1065
BbvI GCAGC 4 cut(s) 10, 645, 798, 903
BccI CCATC 5 cut(s) 100, 328, 508, 668, 1174
BceAI ACGGC 1 cut(s) 386
BciT130I CCWGG 3 cut(s) 384, 525, 1288
BciVI GTATCC 1 cut(s) 1096
BclI TGATCA 1 cut(s) 1138
BcnI CCSGG 1 cut(s) 986
BcoDI GTCTC 3 cut(s) 163, 535, 947
BfaI CTAG 2 cut(s) 471, 659
BfmI CTRYAG 1 cut(s) 450
BfoI RGCGCY 1 cut(s) 873
BfuAI ACCTGC 1 cut(s) 780
BfuI GTATCC 1 cut(s) 1096
BglII AGATCT 3 cut(s) 232, 328, 857
BisI GCNGC 4 cut(s) 24, 634, 787, 892
BlsI GCNGC 4 cut(s) 25, 635, 788, 893
BmcAI AGTACT 1 cut(s) 175
Bme1390I CCNGG 4 cut(s) 384, 525, 986, 1288
BmgBI CACGTC 1 cut(s) 361
BmgT120I GGNCC 1 cut(s) 1305
BmiI GGNNCC 1 cut(s) 1185
BmrFI CCNGG 4 cut(s) 384, 525, 986, 1288
BpiI GAAGAC 1 cut(s) 660
BplI GAGNNNNNCTC 2 cut(s) 307, 339
BpmI CTGGAG 3 cut(s) 269, 309, 339
BpuMI CCSGG 1 cut(s) 986
BsaAI YACGTR 1 cut(s) 464
BsaBI GATNNNNATC 2 cut(s) 574, 1267
BsaJI CCNNGG 2 cut(s) 763, 963
Bsc4I CCNNNNNNNGG 2 cut(s) 290, 1200
Bse1I ACTGG 1 cut(s) 655
Bse3DI GCAATG 3 cut(s) 65, 336, 643
Bse8I GATNNNNATC 2 cut(s) 574, 1267
BseBI CCWGG 3 cut(s) 384, 525, 1288
BseDI CCNNGG 2 cut(s) 763, 963
BseJI GATNNNNATC 2 cut(s) 574, 1267
BseLI CCNNNNNNNGG 2 cut(s) 290, 1200
BseMI GCAATG 3 cut(s) 65, 336, 643
BseMII CTCAG 4 cut(s) 243, 302, 690, 933
BseNI ACTGG 1 cut(s) 655
BseRI GAGGAG 1 cut(s) 328
BseXI GCAGC 4 cut(s) 10, 645, 798, 903
BshFI GGCC 1 cut(s) 1307
BsiHKAI GWGCWC 4 cut(s) 514, 705, 750, 1065
BsiSI CCGG 1 cut(s) 986
BslFI GGGAC 1 cut(s) 265
BslI CCNNNNNNNGG 2 cut(s) 290, 1200
BsmAI GTCTC 3 cut(s) 163, 535, 947
BsmFI GGGAC 1 cut(s) 265
BsmI GAATGC 2 cut(s) 82, 1156
BsnI GGCC 1 cut(s) 1307
Bsp1286I GDGCHC 4 cut(s) 514, 705, 750, 1065
Bsp143I GATC 9 cut(s) 5, 232, 328, 653, 779, 857, 1138, 1247, 1262
Bsp19I CCATGG 1 cut(s) 763
BspACI CCGC 2 cut(s) 245, 663
BspANI GGCC 1 cut(s) 1307
BspCNI CTCAG 4 cut(s) 242, 303, 691, 934
BspLI GGNNCC 1 cut(s) 1185
BspMAI CTGCAG 1 cut(s) 454
BspMI ACCTGC 1 cut(s) 780
BspPI GGATC 5 cut(s) 13, 661, 787, 1255, 1257
BsrDI GCAATG 3 cut(s) 65, 336, 643
BsrI ACTGG 1 cut(s) 655
BssECI CCNNGG 2 cut(s) 763, 963
BssMI GATC 9 cut(s) 5, 232, 328, 653, 779, 857, 1138, 1247, 1262
BssSI CACGAG 2 cut(s) 704, 796
BssT1I CCWWGG 2 cut(s) 763, 963
Bst2BI CACGAG 2 cut(s) 704, 796
Bst2UI CCWGG 3 cut(s) 384, 525, 1288
Bst4CI ACNGT 1 cut(s) 743
Bst6I CTCTTC 1 cut(s) 359
BstBAI YACGTR 1 cut(s) 464
BstC8I GCNNGC 4 cut(s) 13, 245, 889, 991
BstDEI CTNAG 6 cut(s) 229, 311, 699, 773, 942, 1176
BstDSI CCRYGG 1 cut(s) 763
BstH2I RGCGCY 1 cut(s) 873
BstHHI GCGC 1 cut(s) 872
BstKTI GATC 9 cut(s) 8, 235, 331, 656, 782, 860, 1141, 1250, 1265
BstMAI GTCTC 3 cut(s) 163, 535, 947
BstMBI GATC 9 cut(s) 5, 232, 328, 653, 779, 857, 1138, 1247, 1262
BstMWI GCNNNNNNNGC 2 cut(s) 398, 1049
BstNI CCWGG 3 cut(s) 384, 525, 1288
BstSCI CCNGG 4 cut(s) 382, 523, 984, 1286
BstSFI CTRYAG 1 cut(s) 450
BstSNI TACGTA 1 cut(s) 464
BstV1I GCAGC 4 cut(s) 10, 645, 798, 903
BstV2I GAAGAC 1 cut(s) 660
BstX2I RGATCY 5 cut(s) 232, 328, 653, 857, 1262
BstYI RGATCY 5 cut(s) 232, 328, 653, 857, 1262
BsuI GTATCC 1 cut(s) 1096
BsuRI GGCC 1 cut(s) 1307
BtgI CCRYGG 1 cut(s) 763
BtrI CACGTC 1 cut(s) 361
BtsIMutI CAGTG 3 cut(s) 113, 748, 909
BveI ACCTGC 1 cut(s) 780
Cac8I GCNNGC 4 cut(s) 13, 245, 889, 991
CfoI GCGC 1 cut(s) 872
Cfr13I GGNCC 1 cut(s) 1305
Csp6I GTAC 4 cut(s) 174, 465, 620, 690
CviAII CATG 7 cut(s) 96, 187, 290, 424, 764, 864, 938
CviQI GTAC 4 cut(s) 174, 465, 620, 690
DdeI CTNAG 6 cut(s) 229, 311, 699, 773, 942, 1176
DpnI GATC 9 cut(s) 7, 234, 330, 655, 781, 859, 1140, 1249, 1264
DpnII GATC 9 cut(s) 5, 232, 328, 653, 779, 857, 1138, 1247, 1262
DrdI GACNNNNNNGTC 1 cut(s) 513
DseDI GACNNNNNNGTC 1 cut(s) 513
Eam1104I CTCTTC 1 cut(s) 359
EarI CTCTTC 1 cut(s) 359
Ecl136II GAGCTC 3 cut(s) 512, 703, 1063
Eco105I TACGTA 1 cut(s) 464
Eco130I CCWWGG 2 cut(s) 763, 963
Eco24I GRGCYC 3 cut(s) 514, 705, 1065
Eco53kI GAGCTC 3 cut(s) 512, 703, 1063
Eco57I CTGAAG 2 cut(s) 621, 885
EcoICRI GAGCTC 3 cut(s) 512, 703, 1063
EcoRI GAATTC 1 cut(s) 1204
EcoRII CCWGG 3 cut(s) 382, 523, 1286
EcoT14I CCWWGG 2 cut(s) 763, 963
EcoT38I GRGCYC 3 cut(s) 514, 705, 1065
ErhI CCWWGG 2 cut(s) 763, 963
FaeI CATG 7 cut(s) 99, 190, 293, 427, 767, 867, 941
FalI AAGNNNNNCTT 6 cut(s) 718, 750, 1007, 1039, 1208, 1240
FaqI GGGAC 1 cut(s) 265
FatI CATG 7 cut(s) 95, 186, 289, 423, 763, 863, 937
FauI CCCGC 2 cut(s) 252, 656
FbaI TGATCA 1 cut(s) 1138
Fnu4HI GCNGC 4 cut(s) 24, 634, 787, 892
FriOI GRGCYC 3 cut(s) 514, 705, 1065
Fsp4HI GCNGC 4 cut(s) 24, 634, 787, 892
FspBI CTAG 2 cut(s) 471, 659
GlaI GCGC 1 cut(s) 871
GluI GCNGC 4 cut(s) 24, 634, 787, 892
GsuI CTGGAG 3 cut(s) 269, 309, 339
HaeII RGCGCY 1 cut(s) 873
HaeIII GGCC 1 cut(s) 1307
HapII CCGG 1 cut(s) 986
HhaI GCGC 1 cut(s) 872
Hin1II CATG 7 cut(s) 99, 190, 293, 427, 767, 867, 941
Hin6I GCGC 1 cut(s) 870
HinP1I GCGC 1 cut(s) 870
HindIII AAGCTT 1 cut(s) 1041
HinfI GANTC 4 cut(s) 168, 316, 377, 1280
HpaII CCGG 1 cut(s) 986
HphI GGTGA 5 cut(s) 71, 81, 146, 372, 513
Hpy166II GTNNAC 2 cut(s) 521, 926
Hpy188III TCNNGA 5 cut(s) 326, 356, 504, 1035, 1225
Hpy8I GTNNAC 2 cut(s) 521, 926
Hpy99I CGWCG 2 cut(s) 123, 519
HpyAV CCTTC 5 cut(s) 50, 596, 600, 816, 1288
HpyCH4III ACNGT 1 cut(s) 743
HpyCH4IV ACGT 2 cut(s) 360, 463
HpyCH4V TGCA 8 cut(s) 26, 70, 203, 341, 392, 452, 633, 820
HpyF10VI GCNNNNNNNGC 2 cut(s) 398, 1049
HpyF3I CTNAG 6 cut(s) 229, 311, 699, 773, 942, 1176
HpySE526I ACGT 2 cut(s) 360, 463
Hsp92II CATG 7 cut(s) 99, 190, 293, 427, 767, 867, 941
HspAI GCGC 1 cut(s) 870
Ksp22I TGATCA 1 cut(s) 1138
Kzo9I GATC 9 cut(s) 5, 232, 328, 653, 779, 857, 1138, 1247, 1262
LmnI GCTCC 1 cut(s) 867
Lsp1109I GCAGC 4 cut(s) 10, 645, 798, 903
MaeI CTAG 2 cut(s) 471, 659
MaeII ACGT 2 cut(s) 360, 463
MalI GATC 9 cut(s) 7, 234, 330, 655, 781, 859, 1140, 1249, 1264
MboI GATC 9 cut(s) 5, 232, 328, 653, 779, 857, 1138, 1247, 1262
MboII GAAGA 4 cut(s) 376, 660, 852, 1159
MflI RGATCY 5 cut(s) 232, 328, 653, 857, 1262
MhlI GDGCHC 4 cut(s) 514, 705, 750, 1065
MluCI AATT 9 cut(s) 138, 223, 253, 346, 387, 394, 879, 996, 1204
MlyI GAGTC 1 cut(s) 325
MmeI TCCRAC 2 cut(s) 539, 988
MnlI CCTC 7 cut(s) 287, 306, 489, 961, 1041, 1219, 1284
MroXI GAANNNNTTC 2 cut(s) 349, 1220
MseI TTAA 5 cut(s) 194, 206, 572, 848, 1025
MslI CAYNNNNRTG 3 cut(s) 75, 94, 902
MspA1I CMGCKG 2 cut(s) 247, 535
MspI CCGG 1 cut(s) 986
MspR9I CCNGG 4 cut(s) 384, 525, 986, 1288
Mva1269I GAATGC 2 cut(s) 82, 1156
MvaI CCWGG 3 cut(s) 384, 525, 1288
MwoI GCNNNNNNNGC 2 cut(s) 398, 1049
NciI CCSGG 1 cut(s) 986
NcoI CCATGG 1 cut(s) 763
NdeII GATC 9 cut(s) 5, 232, 328, 653, 779, 857, 1138, 1247, 1262
NlaIII CATG 7 cut(s) 99, 190, 293, 427, 767, 867, 941
NlaIV GGNNCC 1 cut(s) 1185
OliI CACNNNNGTG 1 cut(s) 902
PcsI WCGNNNNNNNCGW 1 cut(s) 514
PctI GAATGC 2 cut(s) 82, 1156
PdmI GAANNNNTTC 2 cut(s) 349, 1220
PfeI GAWTC 3 cut(s) 168, 377, 1280
PkrI GCNGC 4 cut(s) 25, 635, 788, 893
PleI GAGTC 1 cut(s) 324
PpsI GAGTC 1 cut(s) 324
Ppu21I YACGTR 1 cut(s) 464
Psp124BI GAGCTC 3 cut(s) 514, 705, 1065
Psp6I CCWGG 3 cut(s) 382, 523, 1286
PspGI CCWGG 3 cut(s) 382, 523, 1286
PspN4I GGNNCC 1 cut(s) 1185
PspPI GGNCC 1 cut(s) 1305
PstI CTGCAG 1 cut(s) 454
PsuI RGATCY 5 cut(s) 232, 328, 653, 857, 1262
PvuII CAGCTG 1 cut(s) 535
RsaI GTAC 4 cut(s) 175, 466, 621, 691
RsaNI GTAC 4 cut(s) 174, 465, 620, 690
RseI CAYNNNNRTG 3 cut(s) 75, 94, 902
SacI GAGCTC 3 cut(s) 514, 705, 1065
SaqAI TTAA 5 cut(s) 194, 206, 572, 848, 1025
SatI GCNGC 4 cut(s) 24, 634, 787, 892
Sau3AI GATC 9 cut(s) 5, 232, 328, 653, 779, 857, 1138, 1247, 1262
Sau96I GGNCC 1 cut(s) 1305
ScaI AGTACT 1 cut(s) 175
SchI GAGTC 1 cut(s) 325
ScrFI CCNGG 4 cut(s) 384, 525, 986, 1288
SduI GDGCHC 4 cut(s) 514, 705, 750, 1065
SfcI CTRYAG 1 cut(s) 450
SmiMI CAYNNNNRTG 3 cut(s) 75, 94, 902
SnaBI TACGTA 1 cut(s) 464
Sse9I AATT 9 cut(s) 138, 223, 253, 346, 387, 394, 879, 996, 1204
SsiI CCGC 2 cut(s) 245, 663
SspMI CTAG 2 cut(s) 471, 659
SstI GAGCTC 3 cut(s) 514, 705, 1065
StyD4I CCNGG 4 cut(s) 382, 523, 984, 1286
StyI CCWWGG 2 cut(s) 763, 963
TaaI ACNGT 1 cut(s) 743
TaiI ACGT 2 cut(s) 363, 466
TaqI TCGA 1 cut(s) 1034
TasI AATT 9 cut(s) 138, 223, 253, 346, 387, 394, 879, 996, 1204
TatI WGTACW 2 cut(s) 173, 619
TfiI GAWTC 3 cut(s) 168, 377, 1280
Tru1I TTAA 5 cut(s) 194, 206, 572, 848, 1025
Tru9I TTAA 5 cut(s) 194, 206, 572, 848, 1025
TscAI CASTG 3 cut(s) 113, 748, 909
TseI GCWGC 4 cut(s) 23, 633, 786, 891
TspDTI ATGAA 3 cut(s) 74, 278, 852
TspGWI ACGGA 1 cut(s) 866
TspRI CASTG 3 cut(s) 113, 748, 909
XapI RAATTY 3 cut(s) 253, 394, 1204
XmnI GAANNNNTTC 2 cut(s) 349, 1220
XspI CTAG 2 cut(s) 471, 659
ZrmI AGTACT 1 cut(s) 175
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.