Rorug05G0221600

Ring finger

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0010718
Physical Location & Seq
Forward (+)
22872321 .. 22874986
2666 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0221600.1

Sequence Viewer

Length: 1332 bp
ATGGGTAAAGAGGAAATAACCCAGAAGTGTACTAGTCCTACTGGTGGCTTTGGTAGATCCTCAAATTCCAAAAAGACTAATAAGCAAAAGAAAGTGCCACAGAGAGGTTTAGGTGTGGCTCAGCTTGAGAAGATCAGATTAGAAGAACAGCAAAAGAAAGCTGCTTCTGTTTTGACATCACCAACCCCACCTGCCTCTAATTCCTTACCATCACCAACCAAATCATCTCCATGTGTTCCTAATCCCGCTCCAATTAGACATTATCAACCACCCTCTTCAATCCCATTTCCATCTCTGCCATCACTGGATCTGCCCTCACCAAACTCGATTTTCAGGCCACCCAATGCCGATGTTGTTCGAAAGCCGAACAGTATTAGTACTGTTCCATTGCCTAACAAGGGGACTCATGGGTTTGAGATTCCGGGACATGGAAATGTTGTTGTTCCCAACTTGTGGAATCCCAATTGTGAGTACAACAGTGCTGATCTCAATAAGGAGAGTTCATCAGGAATTTTGGATCCCGGATTCGCATTTCGGCCTAGTTTGAATAATTTGCCTACTTTCGATAACTCCAACGCGATTTGGGCTCTCCCCAATTTTCCACAAAGAACACACCAATTTCATCACCAACCACCACCACCACCACCTCCTCCTTCATTGGTGAATGTCTCATCAGCTACTTCATTATTGTCATCATCATCTGTACTAAACTTTCACACAGAGCCCCCTTCAAACCAAAGCTATCACAGCAACTATACTACACCAATGTGGTCAGAGGAAGAAAGGATGGTTGCCATGAAAAGGCCATATCCCTTTTCTCACGACAATGAGGCTCCCTCAATCAACTTCAAATTCCCAACCTTTGCTCCTCCTCCTATGAGGACAGATGAAACAACTTCATGTGGGAACAGAGGAGGCACCTTCAATTTAGACCCTGACGGCAACACAATCTTCAGAGAAGGCTCTACCTGCTCAACTTCGATTTCGGAGTCCAATTCGAAGATTAGCATAAAAGAAAATGGGAGTGTTAATGGAGATTTTCTCACACTTGCCCCTCCTACAACTAATAGGACATGTCCGAGCCCAAAGTTTAATTTTCCTCCAGCTTATCTAGCATTCCATAACCCTGAAGTCCCCGATTTCGCCTCATTTCCTTATAAAGGAAGTTCAGAAGATACAATTTTACAGTCAGGACCAAGTGGTTCAACTCCACAACAGGCTTTCTACACCTTCATCCCACCAGCAAAATCACAAATTGTTAAACCAGCAGCAACCATAAACAATTGTAATGGTGAAGTAGGAGAAACTGTTGATCTCAATTTGAAATTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-C3HC4

Protein Analysis

443

Amino Acids

48.0

Weight (kDa)

8.38

Isoelectric Point (pI)

58.88

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010718)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G19680 AT1G75400
fragaria_vesca FvH4_7g07801 FvH4_7g07801 FvH4_7g07801
malus_domestica MD03G1146100.v1.1
pyrus_communis pycom03g10050
rosa_chinensis RchiOBHm_Chr5g0045191
rosa_laevigata RLG00000034342
rosa_multiflora Rmu_co8061458.1_g000001 Rmu_sc0001773.1_g000013
rosa_roxburghii Rroxscaffold_1G00035840
rosa_rugosa Rorug05G0221500.1 Rorug05G0221600
rosa_samantha Rh5AG302900 Rh5BG311400 Rh5CG337400 Rh5DG321600
rosa_wichuraiana Rw5G028110 Rw5G028470

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1158, 1330
AarI CACCTGC 1 cut(s) 199
Acc36I ACCTGC 2 cut(s) 199, 977
AccB1I GGYRCC 1 cut(s) 917
AccB7I CCANNNNNTGG 1 cut(s) 453
AccBSI CCGCTC 1 cut(s) 248
AccII CGCG 1 cut(s) 578
AciI CCGC 1 cut(s) 246
AclWI GGATC 4 cut(s) 51, 315, 512, 525
AcsI RAATTY 3 cut(s) 64, 510, 851
AcuI CTGAAG 2 cut(s) 937, 1149
AfaI GTAC 4 cut(s) 31, 379, 473, 705
AfiI CCNNNNNNNGG 7 cut(s) 44, 104, 398, 428, 453, 801, 1160
AflIII ACRYGT 1 cut(s) 1073
AgsI TTSAA 7 cut(s) 279, 547, 732, 850, 925, 1206, 1324
AhlI ACTAGT 1 cut(s) 32
AleI CACNNNNGTG 1 cut(s) 766
AluBI AGCT 5 cut(s) 124, 161, 677, 741, 1106
AluI AGCT 5 cut(s) 124, 161, 677, 741, 1106
Alw26I GTCTC 1 cut(s) 673
AlwI GGATC 4 cut(s) 51, 315, 512, 525
AoxI GGCC 3 cut(s) 335, 536, 803
ApeKI GCWGC 2 cut(s) 161, 1268
ApoI RAATTY 3 cut(s) 64, 510, 851
AspS9I GGNCC 1 cut(s) 1193
AsuC2I CCSGG 2 cut(s) 423, 522
AsuHPI GGTGA 6 cut(s) 171, 204, 309, 617, 673, 1304
AsuII TTCGAA 2 cut(s) 358, 998
AvaII GGWCC 1 cut(s) 1193
BamHI GGATCC 1 cut(s) 517
BanI GGYRCC 1 cut(s) 917
BanII GRGCYC 3 cut(s) 589, 726, 1085
BbvI GCAGC 2 cut(s) 148, 1280
BccI CCATC 4 cut(s) 217, 298, 307, 781
BceAI ACGGC 1 cut(s) 955
BcnI CCSGG 2 cut(s) 423, 522
BcoDI GTCTC 1 cut(s) 673
BcuI ACTAGT 1 cut(s) 32
BfaI CTAG 3 cut(s) 33, 540, 1112
BfuAI ACCTGC 2 cut(s) 199, 977
BisI GCNGC 2 cut(s) 162, 1269
BlpI GCTNAGC 1 cut(s) 120
BlsI GCNGC 2 cut(s) 163, 1270
BmcAI AGTACT 1 cut(s) 379
Bme1390I CCNGG 2 cut(s) 423, 522
Bme18I GGWCC 1 cut(s) 1193
BmgT120I GGNCC 1 cut(s) 1193
BmiI GGNNCC 3 cut(s) 519, 834, 919
BmrFI CCNGG 2 cut(s) 423, 522
BplI GAGNNNNNCTC 4 cut(s) 821, 853, 1026, 1058
BpmI CTGGAG 1 cut(s) 1086
Bpu1102I GCTNAGC 1 cut(s) 120
Bpu14I TTCGAA 2 cut(s) 358, 998
BpuEI CTTGAG 1 cut(s) 146
BpuMI CCSGG 2 cut(s) 423, 522
BsaXI ACNNNNNCTCC 4 cut(s) 850, 880, 1293, 1323
Bsc4I CCNNNNNNNGG 7 cut(s) 44, 104, 398, 428, 453, 801, 1160
Bse1I ACTGG 2 cut(s) 46, 309
Bse3DI GCAATG 1 cut(s) 386
BseGI GGATG 2 cut(s) 792, 1233
BseLI CCNNNNNNNGG 7 cut(s) 44, 104, 398, 428, 453, 801, 1160
BseMI GCAATG 1 cut(s) 386
BseMII CTCAG 1 cut(s) 134
BseNI ACTGG 2 cut(s) 46, 309
BseRI GAGGAG 4 cut(s) 639, 858, 861, 927
BseXI GCAGC 2 cut(s) 148, 1280
Bsh1236I CGCG 1 cut(s) 578
BshFI GGCC 3 cut(s) 337, 538, 805
BshNI GGYRCC 1 cut(s) 917
BsiSI CCGG 2 cut(s) 422, 522
BslFI GGGAC 3 cut(s) 415, 438, 1118
BslI CCNNNNNNNGG 7 cut(s) 44, 104, 398, 428, 453, 801, 1160
BsmAI GTCTC 1 cut(s) 673
BsmFI GGGAC 3 cut(s) 415, 438, 1118
BsmI GAATGC 1 cut(s) 1115
BsnI GGCC 3 cut(s) 337, 538, 805
Bsp119I TTCGAA 2 cut(s) 358, 998
Bsp1286I GDGCHC 3 cut(s) 589, 726, 1085
Bsp143I GATC 6 cut(s) 56, 132, 307, 484, 517, 1312
Bsp1720I GCTNAGC 1 cut(s) 120
BspACI CCGC 1 cut(s) 246
BspANI GGCC 3 cut(s) 337, 538, 805
BspCNI CTCAG 1 cut(s) 133
BspFNI CGCG 1 cut(s) 578
BspLI GGNNCC 3 cut(s) 519, 834, 919
BspMI ACCTGC 2 cut(s) 199, 977
BspPI GGATC 4 cut(s) 51, 315, 512, 525
BspT104I TTCGAA 2 cut(s) 358, 998
BspT107I GGYRCC 1 cut(s) 917
BsrBI CCGCTC 1 cut(s) 248
BsrDI GCAATG 1 cut(s) 386
BsrI ACTGG 2 cut(s) 46, 309
BssMI GATC 6 cut(s) 56, 132, 307, 484, 517, 1312
Bst4CI ACNGT 5 cut(s) 371, 382, 479, 1188, 1309
Bst6I CTCTTC 1 cut(s) 280
BstBI TTCGAA 2 cut(s) 358, 998
BstDEI CTNAG 1 cut(s) 120
BstENI CCTNNNNNAGG 1 cut(s) 1158
BstF5I GGATG 2 cut(s) 792, 1233
BstFNI CGCG 1 cut(s) 578
BstKTI GATC 6 cut(s) 59, 135, 310, 487, 520, 1315
BstMAI GTCTC 1 cut(s) 673
BstMBI GATC 6 cut(s) 56, 132, 307, 484, 517, 1312
BstMWI GCNNNNNNNGC 4 cut(s) 584, 747, 969, 1112
BstNSI RCATGY 1 cut(s) 1077
BstSCI CCNGG 2 cut(s) 421, 520
BstUI CGCG 1 cut(s) 578
BstV1I GCAGC 2 cut(s) 148, 1280
BstX2I RGATCY 3 cut(s) 56, 307, 517
BstYI RGATCY 3 cut(s) 56, 307, 517
BsuRI GGCC 3 cut(s) 337, 538, 805
BtsCI GGATG 2 cut(s) 792, 1233
BtsIMutI CAGTG 2 cut(s) 302, 484
BveI ACCTGC 2 cut(s) 199, 977
Cfr13I GGNCC 1 cut(s) 1193
Csp6I GTAC 4 cut(s) 30, 378, 472, 704
CviAII CATG 6 cut(s) 231, 407, 428, 796, 900, 1074
CviQI GTAC 4 cut(s) 30, 378, 472, 704
DdeI CTNAG 1 cut(s) 120
DpnI GATC 6 cut(s) 58, 134, 309, 486, 519, 1314
DpnII GATC 6 cut(s) 56, 132, 307, 484, 517, 1312
Eam1104I CTCTTC 1 cut(s) 280
EarI CTCTTC 1 cut(s) 280
Eco24I GRGCYC 3 cut(s) 589, 726, 1085
Eco47I GGWCC 1 cut(s) 1193
Eco57I CTGAAG 2 cut(s) 937, 1149
EcoNI CCTNNNNNAGG 1 cut(s) 1158
EcoT38I GRGCYC 3 cut(s) 589, 726, 1085
FaeI CATG 6 cut(s) 234, 410, 431, 799, 903, 1077
FaqI GGGAC 3 cut(s) 415, 438, 1118
FatI CATG 6 cut(s) 230, 406, 427, 795, 899, 1073
FauI CCCGC 1 cut(s) 253
Fnu4HI GCNGC 2 cut(s) 162, 1269
FokI GGATG 2 cut(s) 799, 1220
FriOI GRGCYC 3 cut(s) 589, 726, 1085
Fsp4HI GCNGC 2 cut(s) 162, 1269
FspBI CTAG 3 cut(s) 33, 540, 1112
GluI GCNGC 2 cut(s) 162, 1269
GsuI CTGGAG 1 cut(s) 1086
HaeIII GGCC 3 cut(s) 337, 538, 805
HapII CCGG 2 cut(s) 422, 522
Hin1II CATG 6 cut(s) 234, 410, 431, 799, 903, 1077
HinfI GANTC 5 cut(s) 403, 418, 457, 525, 989
HpaII CCGG 2 cut(s) 422, 522
HphI GGTGA 6 cut(s) 171, 204, 309, 617, 673, 1304
Hpy166II GTNNAC 1 cut(s) 30
Hpy188I TCNGA 6 cut(s) 137, 775, 956, 988, 1080, 1171
Hpy188III TCNNGA 3 cut(s) 507, 821, 1191
Hpy8I GTNNAC 1 cut(s) 30
HpyAV CCTTC 5 cut(s) 663, 738, 931, 953, 1240
HpyCH4III ACNGT 5 cut(s) 371, 382, 479, 1188, 1309
HpyF10VI GCNNNNNNNGC 4 cut(s) 584, 747, 969, 1112
HpyF3I CTNAG 1 cut(s) 120
Hsp92II CATG 6 cut(s) 234, 410, 431, 799, 903, 1077
Kzo9I GATC 6 cut(s) 56, 132, 307, 484, 517, 1312
LmnI GCTCC 3 cut(s) 253, 838, 871
Lsp1109I GCAGC 2 cut(s) 148, 1280
MaeI CTAG 3 cut(s) 33, 540, 1112
MalI GATC 6 cut(s) 58, 134, 309, 486, 519, 1314
MbiI CCGCTC 1 cut(s) 248
MboI GATC 6 cut(s) 56, 132, 307, 484, 517, 1312
MboII GAAGA 7 cut(s) 142, 155, 267, 791, 943, 1012, 1184
MfeI CAATTG 2 cut(s) 463, 1282
MflI RGATCY 3 cut(s) 56, 307, 517
MhlI GDGCHC 3 cut(s) 589, 726, 1085
MlyI GAGTC 2 cut(s) 397, 998
MmeI TCCRAC 1 cut(s) 597
MseI TTAA 3 cut(s) 1029, 1092, 1260
MslI CAYNNNNRTG 4 cut(s) 229, 432, 766, 825
MspI CCGG 2 cut(s) 422, 522
MspR9I CCNGG 2 cut(s) 423, 522
MunI CAATTG 2 cut(s) 463, 1282
Mva1269I GAATGC 1 cut(s) 1115
MvnI CGCG 1 cut(s) 578
MwoI GCNNNNNNNGC 4 cut(s) 584, 747, 969, 1112
NciI CCSGG 2 cut(s) 423, 522
NdeII GATC 6 cut(s) 56, 132, 307, 484, 517, 1312
NlaIII CATG 6 cut(s) 234, 410, 431, 799, 903, 1077
NlaIV GGNNCC 3 cut(s) 519, 834, 919
NspI RCATGY 1 cut(s) 1077
NspV TTCGAA 2 cut(s) 358, 998
OliI CACNNNNGTG 1 cut(s) 766
PaqCI CACCTGC 1 cut(s) 199
PciI ACATGT 1 cut(s) 1073
PctI GAATGC 1 cut(s) 1115
PfeI GAWTC 3 cut(s) 418, 457, 525
PflMI CCANNNNNTGG 1 cut(s) 453
PfoI TCCNGGA 2 cut(s) 421, 520
PkrI GCNGC 2 cut(s) 163, 1270
PleI GAGTC 2 cut(s) 397, 997
PpsI GAGTC 2 cut(s) 397, 997
PscI ACATGT 1 cut(s) 1073
PsiI TTATAA 2 cut(s) 1158, 1330
PspN4I GGNNCC 3 cut(s) 519, 834, 919
PspPI GGNCC 1 cut(s) 1193
PsuI RGATCY 3 cut(s) 56, 307, 517
RsaI GTAC 4 cut(s) 31, 379, 473, 705
RsaNI GTAC 4 cut(s) 30, 378, 472, 704
RseI CAYNNNNRTG 4 cut(s) 229, 432, 766, 825
SaqAI TTAA 3 cut(s) 1029, 1092, 1260
SatI GCNGC 2 cut(s) 162, 1269
Sau3AI GATC 6 cut(s) 56, 132, 307, 484, 517, 1312
Sau96I GGNCC 1 cut(s) 1193
ScaI AGTACT 1 cut(s) 379
SchI GAGTC 2 cut(s) 397, 998
ScrFI CCNGG 2 cut(s) 423, 522
SduI GDGCHC 3 cut(s) 589, 726, 1085
SfuI TTCGAA 2 cut(s) 358, 998
SinI GGWCC 1 cut(s) 1193
SmiMI CAYNNNNRTG 4 cut(s) 229, 432, 766, 825
SmlI CTYRAG 1 cut(s) 125
SmoI CTYRAG 1 cut(s) 125
SpeI ACTAGT 1 cut(s) 32
SsiI CCGC 1 cut(s) 246
SspMI CTAG 3 cut(s) 33, 540, 1112
StyD4I CCNGG 2 cut(s) 421, 520
TaaI ACNGT 5 cut(s) 371, 382, 479, 1188, 1309
TaqI TCGA 5 cut(s) 326, 358, 564, 980, 998
TatI WGTACW 4 cut(s) 29, 377, 471, 703
TfiI GAWTC 3 cut(s) 418, 457, 525
Tru1I TTAA 3 cut(s) 1029, 1092, 1260
Tru9I TTAA 3 cut(s) 1029, 1092, 1260
TscAI CASTG 2 cut(s) 309, 484
TseI GCWGC 2 cut(s) 161, 1268
TspDTI ATGAA 8 cut(s) 492, 611, 645, 672, 812, 888, 903, 1222
TspRI CASTG 2 cut(s) 309, 484
Van91I CCANNNNNTGG 1 cut(s) 453
VpaK11BI GGWCC 1 cut(s) 1193
XagI CCTNNNNNAGG 1 cut(s) 1158
XapI RAATTY 3 cut(s) 64, 510, 851
XceI RCATGY 1 cut(s) 1077
XspI CTAG 3 cut(s) 33, 540, 1112
ZrmI AGTACT 1 cut(s) 379
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.