MD03G1150400.v1.1

F-box protein At2g27310-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0011837
Physical Location & Seq
Forward (+)
16795804 .. 16796811
1008 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1150400.v1.1.491

Sequence Viewer

Length: 1008 bp
ATGAGCTTTTCAGCTGTAACAACATCGGCCGTCGATCAGGGCGACGGAGCTTCAATCTCCGCCATCCATTCCGACATCATCACTGCTCACATCCTTACCCGCCTCGACGGCCAGACCTTGGCTGCCGCGGCCTGCGCTTCCTCCGAATTACGCGCCTTCTCCGCCGAAGAAAAACTCTGGCGGGACGTCAGCACCTCCACTTGGCCTTCCATCACCGATCCACGTGTCGATGACCTCATTTCCACTTTCCCTGCCGGTCACCGCTCATTCTTCTCCGACTCTTTCCCGCTCCTCGACCACTCCCCCTCCCAATACAATCCCAGATGTTCGTCTCCCACCGCGGAGTTAATCTCGGCCGTCGATATTTTTTACAAAGACCAGCTCATTTTCTCGAAAGTTGAAGAGTCCGAAACCGAGTCCGGGTGGTTCCTCTTCTCGCCCTTCCGAGTTGAATTACTTGACAGGAAAGAAACCGTCCCAACACCAATCCGACATGTCGGGGAAGACCAAAAGTCGTTGAAGAACTTGGAGGACAACTTGAGCCTGAGCTGGATAGTGATCGACCGGGCCCGGAAACGGGCGGCGAACTTGTCGAGCCGGAGGGCGGTGAAGGTGCAGCGGCACTGGCTAACGGGGGAGATACAGCTGCGCTTCGCGACGATTTTGGCGGGGGAGAAGAAGGGGGAGTATGTACAGTGTGGGATGGTGGTCACGTGCAAGGGAAGCGAAGGAGGGGAGCTGCACGTGAGAGAGGTGAGCATGCAGTTGGAGGGAATGGAGGGGAACCACTTGAACGGGAGGGAGAGTTTGGTAATTTTGCAGAGAGCGATCGAGGGAGGGATGAGGAGGACGGAGGTAAACGAGAAGGCGAGGTTCGATGAATACTTGGAGATGAAGAGAGAGAGGAGAGAGAGAAAGGAGAGGAGAGAGAAGGCCTTGGACATGATCTGCATTGCTACTGGGGTTACTATTTTCTTGGCGTTCTGGTCATTTGTCTTGTTCAGATAA

Protein Analysis

336

Amino Acids

37.82

Weight (kDa)

6.12

Isoelectric Point (pI)

44.77

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011837)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G27310
fragaria_vesca FvH4_3g25740
malus_domestica MD03G1150200.v1.1 MD03G1150400.v1.1 MD11G1169600.v1.1 MD11G1169800.v1.1
prunus_persica Prupe.6G137300_v2.0.a1
pyrus_communis pycom03g10430 pycom11g14220
rosa_chinensis RchiOBHm_Chr5g0046101
rosa_laevigata RLG00000034384
rosa_multiflora Rmu_sc0001035.1_g000053
rosa_roxburghii Rroxscaffold_1G00035360
rosa_rugosa Rorug05G0225800
rosa_samantha Rh5AG306500 Rh5BG314900 Rh5CG341200 Rh5DG325100
rosa_wichuraiana Rw5G028660

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 189
AccB7I CCANNNNNTGG 1 cut(s) 118
AccBSI CCGCTC 2 cut(s) 264, 289
AccII CGCG 4 cut(s) 128, 153, 341, 656
AclWI GGATC 1 cut(s) 212
AcoI YGGCCR 3 cut(s) 27, 109, 354
AcvI CACGTG 3 cut(s) 224, 714, 745
AcyI GRCGYC 1 cut(s) 186
AfaI GTAC 1 cut(s) 693
AfiI CCNNNNNNNGG 6 cut(s) 118, 201, 420, 576, 577, 604
AflIII ACRYGT 2 cut(s) 223, 493
AgsI TTSAA 5 cut(s) 54, 401, 452, 520, 793
AhdI GACNNNNNGTC 1 cut(s) 511
AluBI AGCT 7 cut(s) 6, 14, 50, 382, 549, 646, 739
AluI AGCT 7 cut(s) 6, 14, 50, 382, 549, 646, 739
Alw26I GTCTC 1 cut(s) 336
AlwI GGATC 1 cut(s) 212
AoxI GGCC 7 cut(s) 27, 109, 129, 203, 354, 567, 933
ApaI GGGCCC 1 cut(s) 571
ApeKI GCWGC 4 cut(s) 122, 616, 646, 739
AspLEI GCGC 3 cut(s) 137, 155, 651
AspS9I GGNCC 2 cut(s) 567, 568
AsuC2I CCSGG 3 cut(s) 421, 566, 571
AsuHPI GGTGA 4 cut(s) 205, 251, 619, 766
BaeGI GKGCMC 1 cut(s) 571
BanII GRGCYC 1 cut(s) 571
BbrPI CACGTG 3 cut(s) 224, 714, 745
BbsI GAAGAC 1 cut(s) 510
BbvI GCAGC 4 cut(s) 109, 628, 633, 726
BccI CCATC 3 cut(s) 71, 218, 697
BceAI ACGGC 3 cut(s) 14, 124, 341
BcnI CCSGG 3 cut(s) 421, 566, 571
BcoDI GTCTC 1 cut(s) 336
BglI GCCNNNNNGGC 1 cut(s) 108
BisI GCNGC 8 cut(s) 123, 126, 129, 582, 617, 620, 647, 740
BlsI GCNGC 8 cut(s) 124, 127, 130, 583, 618, 621, 648, 741
Bme1390I CCNGG 3 cut(s) 421, 566, 571
BmeRI GACNNNNNGTC 1 cut(s) 511
BmgT120I GGNCC 2 cut(s) 567, 568
BmiI GGNNCC 3 cut(s) 428, 569, 785
BmrFI CCNGG 3 cut(s) 421, 566, 571
BmrI ACTGGG 1 cut(s) 969
BmuI ACTGGG 1 cut(s) 969
BpiI GAAGAC 1 cut(s) 510
BplI GAGNNNNNCTC 2 cut(s) 335, 367
Bpu10I CCTNAGC 1 cut(s) 545
BpuEI CTTGAG 1 cut(s) 559
BpuMI CCSGG 3 cut(s) 421, 566, 571
BsaAI YACGTR 3 cut(s) 224, 714, 745
BsaBI GATNNNNATC 1 cut(s) 557
BsaHI GRCGYC 1 cut(s) 186
BsaJI CCNNGG 4 cut(s) 117, 126, 339, 936
BsaXI ACNNNNNCTCC 4 cut(s) 290, 320, 790, 820
Bsc4I CCNNNNNNNGG 6 cut(s) 118, 201, 420, 576, 577, 604
Bse118I RCCGGY 1 cut(s) 254
Bse1I ACTGG 2 cut(s) 629, 964
Bse3DI GCAATG 1 cut(s) 951
Bse8I GATNNNNATC 1 cut(s) 557
BseDI CCNNGG 4 cut(s) 117, 126, 339, 936
BseGI GGATG 4 cut(s) 63, 90, 708, 846
BseJI GATNNNNATC 1 cut(s) 557
BseLI CCNNNNNNNGG 6 cut(s) 118, 201, 420, 576, 577, 604
BseMI GCAATG 1 cut(s) 951
BseMII CTCAG 1 cut(s) 536
BseNI ACTGG 2 cut(s) 629, 964
BseRI GAGGAG 4 cut(s) 281, 859, 919, 937
BseSI GKGCMC 1 cut(s) 571
BseX3I CGGCCG 2 cut(s) 27, 354
BseXI GCAGC 4 cut(s) 109, 628, 633, 726
BsgI GTGCAG 2 cut(s) 635, 725
Bsh1236I CGCG 4 cut(s) 128, 153, 341, 656
Bsh1285I CGRYCG 4 cut(s) 30, 357, 565, 831
BshFI GGCC 7 cut(s) 29, 111, 131, 205, 356, 569, 935
BsiEI CGRYCG 4 cut(s) 30, 357, 565, 831
BsiSI CCGG 5 cut(s) 255, 420, 565, 571, 598
BslFI GGGAC 2 cut(s) 197, 461
BslI CCNNNNNNNGG 6 cut(s) 118, 201, 420, 576, 577, 604
BsmAI GTCTC 1 cut(s) 336
BsmBI CGTCTC 1 cut(s) 336
BsmFI GGGAC 2 cut(s) 197, 461
BsnI GGCC 7 cut(s) 29, 111, 131, 205, 356, 569, 935
Bsp120I GGGCCC 1 cut(s) 567
Bsp1286I GDGCHC 1 cut(s) 571
Bsp1407I TGTACA 1 cut(s) 691
Bsp143I GATC 5 cut(s) 34, 217, 558, 828, 945
Bsp68I TCGCGA 1 cut(s) 656
BspANI GGCC 7 cut(s) 29, 111, 131, 205, 356, 569, 935
BspCNI CTCAG 1 cut(s) 537
BspFNI CGCG 4 cut(s) 128, 153, 341, 656
BspLI GGNNCC 3 cut(s) 428, 569, 785
BspPI GGATC 1 cut(s) 212
BsrBI CCGCTC 2 cut(s) 264, 289
BsrDI GCAATG 1 cut(s) 951
BsrFI RCCGGY 1 cut(s) 254
BsrGI TGTACA 1 cut(s) 691
BsrI ACTGG 2 cut(s) 629, 964
BssAI RCCGGY 1 cut(s) 254
BssECI CCNNGG 4 cut(s) 117, 126, 339, 936
BssMI GATC 5 cut(s) 34, 217, 558, 828, 945
BssNI GRCGYC 1 cut(s) 186
BssT1I CCWWGG 2 cut(s) 117, 936
Bst4CI ACNGT 2 cut(s) 475, 696
Bst6I CTCTTC 3 cut(s) 396, 437, 890
BstACI GRCGYC 1 cut(s) 186
BstAUI TGTACA 1 cut(s) 691
BstBAI YACGTR 3 cut(s) 224, 714, 745
BstC8I GCNNGC 2 cut(s) 133, 761
BstDEI CTNAG 1 cut(s) 545
BstDSI CCRYGG 2 cut(s) 126, 339
BstEII GGTNACC 1 cut(s) 257
BstF5I GGATG 4 cut(s) 63, 90, 708, 846
BstFNI CGCG 4 cut(s) 128, 153, 341, 656
BstHHI GCGC 3 cut(s) 137, 155, 651
BstKTI GATC 5 cut(s) 37, 220, 561, 831, 948
BstMAI GTCTC 1 cut(s) 336
BstMBI GATC 5 cut(s) 34, 217, 558, 828, 945
BstMCI CGRYCG 4 cut(s) 30, 357, 565, 831
BstMWI GCNNNNNNNGC 6 cut(s) 108, 128, 134, 161, 625, 723
BstNSI RCATGY 2 cut(s) 497, 763
BstPI GGTNACC 1 cut(s) 257
BstSCI CCNGG 3 cut(s) 419, 564, 569
BstSLI GKGCMC 1 cut(s) 571
BstUI CGCG 4 cut(s) 128, 153, 341, 656
BstV1I GCAGC 4 cut(s) 109, 628, 633, 726
BstV2I GAAGAC 1 cut(s) 510
BstZI CGGCCG 2 cut(s) 27, 354
BsuRI GGCC 7 cut(s) 29, 111, 131, 205, 356, 569, 935
BtgI CCRYGG 2 cut(s) 126, 339
BtsCI GGATG 4 cut(s) 63, 90, 708, 846
BtsI GCAGTG 1 cut(s) 81
BtsIMutI CAGTG 3 cut(s) 81, 622, 701
BtuMI TCGCGA 1 cut(s) 656
Cac8I GCNNGC 2 cut(s) 133, 761
CfoI GCGC 3 cut(s) 137, 155, 651
Cfr10I RCCGGY 1 cut(s) 254
Cfr13I GGNCC 2 cut(s) 567, 568
Cfr42I CCGCGG 2 cut(s) 129, 342
Csp6I GTAC 1 cut(s) 692
CviAII CATG 3 cut(s) 494, 760, 943
CviQI GTAC 1 cut(s) 692
DdeI CTNAG 1 cut(s) 545
DpnI GATC 5 cut(s) 36, 219, 560, 830, 947
DpnII GATC 5 cut(s) 34, 217, 558, 828, 945
DriI GACNNNNNGTC 1 cut(s) 511
EaeI YGGCCR 3 cut(s) 27, 109, 354
EagI CGGCCG 2 cut(s) 27, 354
Eam1104I CTCTTC 3 cut(s) 396, 437, 890
Eam1105I GACNNNNNGTC 1 cut(s) 511
EarI CTCTTC 3 cut(s) 396, 437, 890
EciI GGCGGA 2 cut(s) 49, 151
EclXI CGGCCG 2 cut(s) 27, 354
Eco130I CCWWGG 2 cut(s) 117, 936
Eco147I AGGCCT 1 cut(s) 935
Eco24I GRGCYC 1 cut(s) 571
Eco52I CGGCCG 2 cut(s) 27, 354
Eco72I CACGTG 3 cut(s) 224, 714, 745
Eco91I GGTNACC 1 cut(s) 257
EcoO65I GGTNACC 1 cut(s) 257
EcoT14I CCWWGG 2 cut(s) 117, 936
EcoT38I GRGCYC 1 cut(s) 571
ErhI CCWWGG 2 cut(s) 117, 936
Esp3I CGTCTC 1 cut(s) 336
FaeI CATG 3 cut(s) 497, 763, 946
FaiI YATR 4 cut(s) 495, 690, 761, 944
FaqI GGGAC 2 cut(s) 197, 461
FatI CATG 3 cut(s) 493, 759, 942
FauI CCCGC 4 cut(s) 107, 174, 294, 661
Fnu4HI GCNGC 8 cut(s) 123, 126, 129, 582, 617, 620, 647, 740
FokI GGATG 4 cut(s) 50, 77, 715, 853
FriOI GRGCYC 1 cut(s) 571
Fsp4HI GCNGC 8 cut(s) 123, 126, 129, 582, 617, 620, 647, 740
GlaI GCGC 3 cut(s) 136, 154, 650
GluI GCNGC 8 cut(s) 123, 126, 129, 582, 617, 620, 647, 740
HaeIII GGCC 7 cut(s) 29, 111, 131, 205, 356, 569, 935
HapII CCGG 5 cut(s) 255, 420, 565, 571, 598
HhaI GCGC 3 cut(s) 137, 155, 651
Hin1I GRCGYC 1 cut(s) 186
Hin1II CATG 3 cut(s) 497, 763, 946
Hin6I GCGC 3 cut(s) 135, 153, 649
HinP1I GCGC 3 cut(s) 135, 153, 649
HinfI GANTC 3 cut(s) 278, 404, 416
HpaII CCGG 5 cut(s) 255, 420, 565, 571, 598
HphI GGTGA 4 cut(s) 205, 251, 619, 766
Hpy166II GTNNAC 1 cut(s) 859
Hpy188I TCNGA 7 cut(s) 73, 145, 277, 409, 446, 491, 1004
Hpy188III TCNNGA 2 cut(s) 391, 655
Hpy8I GTNNAC 1 cut(s) 859
Hpy99I CGWCG 5 cut(s) 35, 47, 110, 362, 661
HpyAV CCTTC 8 cut(s) 166, 216, 451, 604, 673, 722, 859, 925
HpyCH4III ACNGT 2 cut(s) 475, 696
HpyCH4IV ACGT 4 cut(s) 186, 223, 713, 744
HpyCH4V TGCA 6 cut(s) 616, 717, 742, 763, 820, 951
HpyF10VI GCNNNNNNNGC 6 cut(s) 108, 128, 134, 161, 625, 723
HpyF3I CTNAG 1 cut(s) 545
HpySE526I ACGT 4 cut(s) 186, 223, 713, 744
Hsp92I GRCGYC 1 cut(s) 186
Hsp92II CATG 3 cut(s) 497, 763, 946
HspAI GCGC 3 cut(s) 135, 153, 649
KspI CCGCGG 2 cut(s) 129, 342
Kzo9I GATC 5 cut(s) 34, 217, 558, 828, 945
LmnI GCTCC 3 cut(s) 47, 294, 736
Lsp1109I GCAGC 4 cut(s) 109, 628, 633, 726
MaeII ACGT 4 cut(s) 186, 223, 713, 744
MaeIII GTNAC 4 cut(s) 16, 257, 709, 964
MalI GATC 5 cut(s) 36, 219, 560, 830, 947
MbiI CCGCTC 2 cut(s) 264, 289
MboI GATC 5 cut(s) 34, 217, 558, 828, 945
MboII GAAGA 8 cut(s) 179, 262, 413, 424, 515, 532, 688, 907
MhlI GDGCHC 1 cut(s) 571
MluCI AATT 3 cut(s) 146, 452, 813
MlyI GAGTC 3 cut(s) 272, 413, 425
MmeI TCCRAC 4 cut(s) 96, 300, 514, 747
MseI TTAA 1 cut(s) 347
MspA1I CMGCKG 5 cut(s) 14, 128, 341, 619, 646
MspI CCGG 5 cut(s) 255, 420, 565, 571, 598
MspR9I CCNGG 3 cut(s) 421, 566, 571
MvnI CGCG 4 cut(s) 128, 153, 341, 656
MwoI GCNNNNNNNGC 6 cut(s) 108, 128, 134, 161, 625, 723
NciI CCSGG 3 cut(s) 421, 566, 571
NdeII GATC 5 cut(s) 34, 217, 558, 828, 945
NlaIII CATG 3 cut(s) 497, 763, 946
NlaIV GGNNCC 3 cut(s) 428, 569, 785
NmeAIII GCCGAG 1 cut(s) 332
NmuCI GTSAC 2 cut(s) 257, 709
NruI TCGCGA 1 cut(s) 656
NspI RCATGY 2 cut(s) 497, 763
PaeI GCATGC 1 cut(s) 763
PceI AGGCCT 1 cut(s) 935
PciI ACATGT 1 cut(s) 493
PcsI WCGNNNNNNNCGW 1 cut(s) 39
PflMI CCANNNNNTGG 1 cut(s) 118
PkrI GCNGC 8 cut(s) 124, 127, 130, 583, 618, 621, 648, 741
Ple19I CGATCG 1 cut(s) 831
PleI GAGTC 3 cut(s) 272, 412, 424
PmaCI CACGTG 3 cut(s) 224, 714, 745
PmlI CACGTG 3 cut(s) 224, 714, 745
PpsI GAGTC 3 cut(s) 272, 412, 424
Ppu21I YACGTR 3 cut(s) 224, 714, 745
PscI ACATGT 1 cut(s) 493
PspCI CACGTG 3 cut(s) 224, 714, 745
PspEI GGTNACC 1 cut(s) 257
PspN4I GGNNCC 3 cut(s) 428, 569, 785
PspOMI GGGCCC 1 cut(s) 567
PspPI GGNCC 2 cut(s) 567, 568
PvuI CGATCG 1 cut(s) 831
PvuII CAGCTG 2 cut(s) 14, 646
RruI TCGCGA 1 cut(s) 656
RsaI GTAC 1 cut(s) 693
RsaNI GTAC 1 cut(s) 692
SacII CCGCGG 2 cut(s) 129, 342
SaqAI TTAA 1 cut(s) 347
SatI GCNGC 8 cut(s) 123, 126, 129, 582, 617, 620, 647, 740
Sau3AI GATC 5 cut(s) 34, 217, 558, 828, 945
Sau96I GGNCC 2 cut(s) 567, 568
SchI GAGTC 3 cut(s) 272, 413, 425
ScrFI CCNGG 3 cut(s) 421, 566, 571
SduI GDGCHC 1 cut(s) 571
Sfr303I CCGCGG 2 cut(s) 129, 342
SgrBI CCGCGG 2 cut(s) 129, 342
SmlI CTYRAG 1 cut(s) 538
SmoI CTYRAG 1 cut(s) 538
SphI GCATGC 1 cut(s) 763
Sse9I AATT 3 cut(s) 146, 452, 813
SseBI AGGCCT 1 cut(s) 935
StuI AGGCCT 1 cut(s) 935
StyD4I CCNGG 3 cut(s) 419, 564, 569
StyI CCWWGG 2 cut(s) 117, 936
TaaI ACNGT 2 cut(s) 475, 696
TaiI ACGT 4 cut(s) 189, 226, 716, 747
TasI AATT 3 cut(s) 146, 452, 813
TatI WGTACW 1 cut(s) 691
TauI GCSGC 4 cut(s) 128, 131, 584, 622
Tru1I TTAA 1 cut(s) 347
Tru9I TTAA 1 cut(s) 347
TscAI CASTG 3 cut(s) 88, 629, 701
TseFI GTSAC 2 cut(s) 257, 709
TseI GCWGC 4 cut(s) 122, 616, 646, 739
Tsp45I GTSAC 2 cut(s) 257, 709
TspDTI ATGAA 2 cut(s) 894, 908
TspGWI ACGGA 2 cut(s) 60, 866
TspRI CASTG 3 cut(s) 88, 629, 701
Van91I CCANNNNNTGG 1 cut(s) 118
XceI RCATGY 2 cut(s) 497, 763
ZraI GACGTC 1 cut(s) 187
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.