pycom03g10430

F-box protein At2g27310-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr3
OG0011837
Physical Location & Seq
Forward (+)
9600773 .. 9601771
999 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom03g10430.1

Sequence Viewer

Length: 999 bp
ATGAGCTTTTCTGTACCGGCTGTCGATCATGGCGACGGAGCTTCAATCTCCTCCGTCCATTCCGACATCATCACGGCTCACATCCTCACCCGCCTCGACGGCCAGACCTTGGCCTCCGCCGCCTGCGCTTCCTCCGAATTACACGCCTGCTCCGCCGAAGAAAAACTCTGGCGGGACGTCAACACCACCATGTGGCCTTCAATAACCGATCCTCGAGTCGACGACCTCATTTCCACTTTCCCTGCCGGTCACCGCTCTTTCTTCTCAGACTCGTTCCCGCTCCTCGACTACTCCCCCTCCCAATTCGTTTTCAGCCCATCGTGTCCCACCACGGAGTTAATCTCCGCCGTCGATATTTTTTACAAAGGCCAGGTCATTTTCTCGAAAGTTCAAGAGTCCGAGACCGAGTCCGGGTGGTTCCTCTGCTCGCCCTTCCGAGTTGACTTACTTGACCAAAAAGAAACCGTCCCAACACCAATCCGACATGTCGGGGAAGACCAGAAGTTGTTGGAGCACTTGGAGGACAACTTGAGCCTGAGCTGGATCGTGATCGACCGGGCCCGGAAACGCGCGGCGAACTTGTCGAGCCGGAGGGCGGTGACGGTGCAGCGGCACTGGCTAACGGGGGAGATACAGCTGCGCTTCGCGACGATTTTGGAGGCAGAGAAGAAGGGGGAGTATGTGCAGTGCGGGATGGTGGTCATGTGCAAGGGAAGCGAAGGAGGGGAGCTGCACGTGAGAGAGGTGAGCATGAAGGTGGAAGGAATGGAGGGGAATCACTTGAACGGGAGGGAGAGTTTGTTACTTCTGCAGAGGGCGATCGAGGGAGGGAAGAGGAGGAAGGAGGTAAACGGGAAGGCGAGATTCGATGAGTACTTGGAGATGAAGAGAGAGAGGAGAGAGAGAAAGGAGAGGAGAGAGAAGGCTTTGGACATGATCTGCATTGTTACTGGGGTTACTATTTTGATAGCGTTCTGGTCATTTGTCTTGTTCAGATAG

Protein Analysis

333

Amino Acids

37.52

Weight (kDa)

6.1

Isoelectric Point (pI)

48.15

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011837)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G27310
fragaria_vesca FvH4_3g25740
malus_domestica MD03G1150200.v1.1 MD03G1150400.v1.1 MD11G1169600.v1.1 MD11G1169800.v1.1
prunus_persica Prupe.6G137300_v2.0.a1
pyrus_communis pycom03g10430 pycom11g14220
rosa_chinensis RchiOBHm_Chr5g0046101
rosa_laevigata RLG00000034384
rosa_multiflora Rmu_sc0001035.1_g000053
rosa_roxburghii Rroxscaffold_1G00035360
rosa_rugosa Rorug05G0225800
rosa_samantha Rh5AG306500 Rh5BG314900 Rh5CG341200 Rh5DG325100
rosa_wichuraiana Rw5G028660

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 180
AccB7I CCANNNNNTGG 2 cut(s) 109, 192
AccBSI CCGCTC 2 cut(s) 255, 280
AccI GTMKAC 1 cut(s) 219
AccII CGCG 3 cut(s) 570, 572, 647
AclWI GGATC 2 cut(s) 203, 551
AcoI YGGCCR 1 cut(s) 100
AcvI CACGTG 1 cut(s) 736
AcyI GRCGYC 1 cut(s) 177
AdeI CACNNNGTG 1 cut(s) 192
AfaI GTAC 2 cut(s) 15, 875
AfiI CCNNNNNNNGG 4 cut(s) 109, 192, 411, 595
AflIII ACRYGT 1 cut(s) 484
AgsI TTSAA 4 cut(s) 45, 201, 392, 784
AjnI CCWGG 1 cut(s) 369
AluBI AGCT 5 cut(s) 6, 41, 540, 637, 730
AluI AGCT 5 cut(s) 6, 41, 540, 637, 730
Alw21I GWGCWC 1 cut(s) 516
Alw26I GTCTC 1 cut(s) 395
AlwI GGATC 2 cut(s) 203, 551
Ama87I CYCGRG 1 cut(s) 213
AoxI GGCC 5 cut(s) 100, 111, 194, 367, 558
ApaI GGGCCC 1 cut(s) 562
ApeKI GCWGC 3 cut(s) 607, 637, 730
ArsI GACNNNNNNTTYG 2 cut(s) 357, 389
AspLEI GCGC 3 cut(s) 128, 572, 642
AspS9I GGNCC 2 cut(s) 558, 559
AsuC2I CCSGG 3 cut(s) 412, 557, 562
AsuHPI GGTGA 4 cut(s) 79, 242, 610, 757
AvaI CYCGRG 1 cut(s) 213
BaeGI GKGCMC 1 cut(s) 562
BanII GRGCYC 1 cut(s) 562
BbrPI CACGTG 1 cut(s) 736
BbsI GAAGAC 1 cut(s) 501
Bbv12I GWGCWC 1 cut(s) 516
BbvI GCAGC 3 cut(s) 619, 624, 717
BccI CCATC 2 cut(s) 325, 688
BceAI ACGGC 3 cut(s) 90, 115, 332
BciT130I CCWGG 1 cut(s) 371
BcnI CCSGG 3 cut(s) 412, 557, 562
BcoDI GTCTC 1 cut(s) 395
BfmI CTRYAG 1 cut(s) 809
BglI GCCNNNNNGGC 1 cut(s) 99
BisI GCNGC 6 cut(s) 120, 573, 608, 611, 638, 731
BlsI GCNGC 6 cut(s) 121, 574, 609, 612, 639, 732
BmcAI AGTACT 1 cut(s) 875
Bme1390I CCNGG 4 cut(s) 371, 412, 557, 562
BmeT110I CYCGRG 1 cut(s) 213
BmgT120I GGNCC 2 cut(s) 558, 559
BmiI GGNNCC 2 cut(s) 419, 560
BmrFI CCNGG 4 cut(s) 371, 412, 557, 562
BmrI ACTGGG 1 cut(s) 960
BmuI ACTGGG 1 cut(s) 960
BpiI GAAGAC 1 cut(s) 501
BplI GAGNNNNNCTC 2 cut(s) 326, 358
Bpu10I CCTNAGC 1 cut(s) 536
BpuEI CTTGAG 1 cut(s) 550
BpuMI CCSGG 3 cut(s) 412, 557, 562
BsaAI YACGTR 1 cut(s) 736
BsaBI GATNNNNATC 1 cut(s) 548
BsaHI GRCGYC 1 cut(s) 177
BsaI GGTCTC 1 cut(s) 395
BsaJI CCNNGG 2 cut(s) 108, 330
BsaXI ACNNNNNCTCC 8 cut(s) 134, 164, 281, 311, 781, 785, 811, 815
Bsc4I CCNNNNNNNGG 4 cut(s) 109, 192, 411, 595
Bse118I RCCGGY 2 cut(s) 16, 245
Bse1I ACTGG 2 cut(s) 620, 955
Bse8I GATNNNNATC 1 cut(s) 548
BseBI CCWGG 1 cut(s) 371
BseDI CCNNGG 2 cut(s) 108, 330
BseGI GGATG 2 cut(s) 81, 699
BseJI GATNNNNATC 1 cut(s) 548
BseLI CCNNNNNNNGG 4 cut(s) 109, 192, 411, 595
BseMII CTCAG 2 cut(s) 279, 527
BseNI ACTGG 2 cut(s) 620, 955
BseRI GAGGAG 5 cut(s) 40, 272, 850, 910, 928
BseSI GKGCMC 1 cut(s) 562
BseXI GCAGC 3 cut(s) 619, 624, 717
BsgI GTGCAG 3 cut(s) 626, 704, 716
Bsh1236I CGCG 3 cut(s) 570, 572, 647
Bsh1285I CGRYCG 2 cut(s) 556, 822
BshFI GGCC 5 cut(s) 102, 113, 196, 369, 560
BsiEI CGRYCG 2 cut(s) 556, 822
BsiHKAI GWGCWC 1 cut(s) 516
BsiHKCI CYCGRG 1 cut(s) 213
BsiSI CCGG 6 cut(s) 17, 246, 411, 556, 562, 589
BslFI GGGAC 3 cut(s) 188, 309, 452
BslI CCNNNNNNNGG 4 cut(s) 109, 192, 411, 595
BsmAI GTCTC 1 cut(s) 395
BsmFI GGGAC 3 cut(s) 188, 309, 452
BsnI GGCC 5 cut(s) 102, 113, 196, 369, 560
Bso31I GGTCTC 1 cut(s) 395
BsoBI CYCGRG 1 cut(s) 213
Bsp120I GGGCCC 1 cut(s) 558
Bsp1286I GDGCHC 2 cut(s) 516, 562
Bsp143I GATC 6 cut(s) 25, 208, 543, 549, 819, 936
Bsp68I TCGCGA 1 cut(s) 647
BspANI GGCC 5 cut(s) 102, 113, 196, 369, 560
BspCNI CTCAG 2 cut(s) 278, 528
BspFNI CGCG 3 cut(s) 570, 572, 647
BspLI GGNNCC 2 cut(s) 419, 560
BspMAI CTGCAG 1 cut(s) 813
BspPI GGATC 2 cut(s) 203, 551
BspTNI GGTCTC 1 cut(s) 395
BsrBI CCGCTC 2 cut(s) 255, 280
BsrFI RCCGGY 2 cut(s) 16, 245
BsrI ACTGG 2 cut(s) 620, 955
BssAI RCCGGY 2 cut(s) 16, 245
BssECI CCNNGG 2 cut(s) 108, 330
BssMI GATC 6 cut(s) 25, 208, 543, 549, 819, 936
BssNI GRCGYC 1 cut(s) 177
BssT1I CCWWGG 1 cut(s) 108
Bst2UI CCWGG 1 cut(s) 371
Bst4CI ACNGT 2 cut(s) 466, 604
Bst6I CTCTTC 2 cut(s) 827, 881
BstACI GRCGYC 1 cut(s) 177
BstBAI YACGTR 1 cut(s) 736
BstC8I GCNNGC 3 cut(s) 124, 148, 428
BstDEI CTNAG 2 cut(s) 265, 536
BstDSI CCRYGG 1 cut(s) 330
BstEII GGTNACC 1 cut(s) 248
BstF5I GGATG 2 cut(s) 81, 699
BstFNI CGCG 3 cut(s) 570, 572, 647
BstHHI GCGC 3 cut(s) 128, 572, 642
BstKTI GATC 6 cut(s) 28, 211, 546, 552, 822, 939
BstMAI GTCTC 1 cut(s) 395
BstMBI GATC 6 cut(s) 25, 208, 543, 549, 819, 936
BstMCI CGRYCG 2 cut(s) 556, 822
BstMWI GCNNNNNNNGC 6 cut(s) 99, 119, 125, 152, 616, 714
BstNI CCWGG 1 cut(s) 371
BstNSI RCATGY 1 cut(s) 488
BstPI GGTNACC 1 cut(s) 248
BstSCI CCNGG 4 cut(s) 369, 410, 555, 560
BstSFI CTRYAG 1 cut(s) 809
BstSLI GKGCMC 1 cut(s) 562
BstUI CGCG 3 cut(s) 570, 572, 647
BstV1I GCAGC 3 cut(s) 619, 624, 717
BstV2I GAAGAC 1 cut(s) 501
BsuRI GGCC 5 cut(s) 102, 113, 196, 369, 560
BtgI CCRYGG 1 cut(s) 330
BtsCI GGATG 2 cut(s) 81, 699
BtsI GCAGTG 1 cut(s) 692
BtsIMutI CAGTG 2 cut(s) 613, 692
BtuMI TCGCGA 1 cut(s) 647
Cac8I GCNNGC 3 cut(s) 124, 148, 428
CfoI GCGC 3 cut(s) 128, 572, 642
Cfr10I RCCGGY 2 cut(s) 16, 245
Cfr13I GGNCC 2 cut(s) 558, 559
Csp6I GTAC 2 cut(s) 14, 874
CviAII CATG 6 cut(s) 29, 190, 485, 703, 751, 934
CviQI GTAC 2 cut(s) 14, 874
DdeI CTNAG 2 cut(s) 265, 536
DpnI GATC 6 cut(s) 27, 210, 545, 551, 821, 938
DpnII GATC 6 cut(s) 25, 208, 543, 549, 819, 936
DraIII CACNNNGTG 1 cut(s) 192
EaeI YGGCCR 1 cut(s) 100
Eam1104I CTCTTC 2 cut(s) 827, 881
EarI CTCTTC 2 cut(s) 827, 881
EciI GGCGGA 3 cut(s) 106, 142, 334
Eco130I CCWWGG 1 cut(s) 108
Eco24I GRGCYC 1 cut(s) 562
Eco31I GGTCTC 1 cut(s) 395
Eco72I CACGTG 1 cut(s) 736
Eco88I CYCGRG 1 cut(s) 213
Eco91I GGTNACC 1 cut(s) 248
EcoO65I GGTNACC 1 cut(s) 248
EcoRII CCWGG 1 cut(s) 369
EcoT14I CCWWGG 1 cut(s) 108
EcoT38I GRGCYC 1 cut(s) 562
ErhI CCWWGG 1 cut(s) 108
FaeI CATG 6 cut(s) 32, 193, 488, 706, 754, 937
FaiI YATR 7 cut(s) 30, 191, 486, 681, 704, 752, 935
FaqI GGGAC 3 cut(s) 188, 309, 452
FatI CATG 6 cut(s) 28, 189, 484, 702, 750, 933
FauI CCCGC 4 cut(s) 98, 165, 285, 683
FblI GTMKAC 1 cut(s) 219
Fnu4HI GCNGC 6 cut(s) 120, 573, 608, 611, 638, 731
FokI GGATG 2 cut(s) 68, 706
FriOI GRGCYC 1 cut(s) 562
Fsp4HI GCNGC 6 cut(s) 120, 573, 608, 611, 638, 731
GlaI GCGC 3 cut(s) 127, 571, 641
GluI GCNGC 6 cut(s) 120, 573, 608, 611, 638, 731
HaeIII GGCC 5 cut(s) 102, 113, 196, 369, 560
HapII CCGG 6 cut(s) 17, 246, 411, 556, 562, 589
HhaI GCGC 3 cut(s) 128, 572, 642
Hin1I GRCGYC 1 cut(s) 177
Hin1II CATG 6 cut(s) 32, 193, 488, 706, 754, 937
Hin6I GCGC 3 cut(s) 126, 570, 640
HinP1I GCGC 3 cut(s) 126, 570, 640
HincII GTYRAC 3 cut(s) 181, 220, 442
HindII GTYRAC 3 cut(s) 181, 220, 442
HinfI GANTC 6 cut(s) 216, 269, 395, 407, 775, 864
HpaII CCGG 6 cut(s) 17, 246, 411, 556, 562, 589
HphI GGTGA 4 cut(s) 79, 242, 610, 757
Hpy166II GTNNAC 4 cut(s) 181, 220, 442, 850
Hpy188I TCNGA 7 cut(s) 64, 136, 268, 400, 437, 482, 995
Hpy188III TCNNGA 4 cut(s) 382, 392, 547, 646
Hpy8I GTNNAC 4 cut(s) 181, 220, 442, 850
Hpy99I CGWCG 5 cut(s) 38, 101, 224, 353, 652
HpyAV CCTTC 9 cut(s) 207, 442, 664, 713, 748, 755, 835, 850, 916
HpyCH4III ACNGT 2 cut(s) 466, 604
HpyCH4IV ACGT 2 cut(s) 177, 735
HpyCH4V TGCA 6 cut(s) 607, 685, 708, 733, 811, 942
HpyF10VI GCNNNNNNNGC 6 cut(s) 99, 119, 125, 152, 616, 714
HpyF3I CTNAG 2 cut(s) 265, 536
HpySE526I ACGT 2 cut(s) 177, 735
Hsp92I GRCGYC 1 cut(s) 177
Hsp92II CATG 6 cut(s) 32, 193, 488, 706, 754, 937
HspAI GCGC 3 cut(s) 126, 570, 640
Kzo9I GATC 6 cut(s) 25, 208, 543, 549, 819, 936
LmnI GCTCC 5 cut(s) 38, 155, 285, 511, 727
Lsp1109I GCAGC 3 cut(s) 619, 624, 717
MaeII ACGT 2 cut(s) 177, 735
MaeIII GTNAC 5 cut(s) 248, 598, 801, 946, 955
MalI GATC 6 cut(s) 27, 210, 545, 551, 821, 938
MbiI CCGCTC 2 cut(s) 255, 280
MboI GATC 6 cut(s) 25, 208, 543, 549, 819, 936
MboII GAAGA 6 cut(s) 170, 253, 506, 679, 844, 898
MhlI GDGCHC 2 cut(s) 516, 562
MluCI AATT 2 cut(s) 137, 302
MlyI GAGTC 4 cut(s) 225, 263, 404, 416
MmeI TCCRAC 3 cut(s) 87, 489, 505
MseI TTAA 1 cut(s) 338
MslI CAYNNNNRTG 2 cut(s) 188, 755
MspA1I CMGCKG 2 cut(s) 610, 637
MspI CCGG 6 cut(s) 17, 246, 411, 556, 562, 589
MspR9I CCNGG 4 cut(s) 371, 412, 557, 562
MvaI CCWGG 1 cut(s) 371
MvnI CGCG 3 cut(s) 570, 572, 647
MwoI GCNNNNNNNGC 6 cut(s) 99, 119, 125, 152, 616, 714
NciI CCSGG 3 cut(s) 412, 557, 562
NdeII GATC 6 cut(s) 25, 208, 543, 549, 819, 936
NlaIII CATG 6 cut(s) 32, 193, 488, 706, 754, 937
NlaIV GGNNCC 2 cut(s) 419, 560
NmuCI GTSAC 2 cut(s) 248, 598
NruI TCGCGA 1 cut(s) 647
NspI RCATGY 1 cut(s) 488
PaeR7I CTCGAG 1 cut(s) 213
PciI ACATGT 1 cut(s) 484
PcsI WCGNNNNNNNCGW 1 cut(s) 30
PfeI GAWTC 2 cut(s) 775, 864
PflFI GACNNNGTC 1 cut(s) 406
PflMI CCANNNNNTGG 2 cut(s) 109, 192
PkrI GCNGC 6 cut(s) 121, 574, 609, 612, 639, 732
Ple19I CGATCG 1 cut(s) 822
PleI GAGTC 4 cut(s) 224, 263, 403, 415
PmaCI CACGTG 1 cut(s) 736
PmlI CACGTG 1 cut(s) 736
PpsI GAGTC 4 cut(s) 224, 263, 403, 415
Ppu21I YACGTR 1 cut(s) 736
PscI ACATGT 1 cut(s) 484
Psp6I CCWGG 1 cut(s) 369
PspCI CACGTG 1 cut(s) 736
PspEI GGTNACC 1 cut(s) 248
PspGI CCWGG 1 cut(s) 369
PspN4I GGNNCC 2 cut(s) 419, 560
PspOMI GGGCCC 1 cut(s) 558
PspPI GGNCC 2 cut(s) 558, 559
PspXI VCTCGAGB 1 cut(s) 213
PstI CTGCAG 1 cut(s) 813
PsyI GACNNNGTC 1 cut(s) 406
PvuI CGATCG 1 cut(s) 822
PvuII CAGCTG 1 cut(s) 637
RruI TCGCGA 1 cut(s) 647
RsaI GTAC 2 cut(s) 15, 875
RsaNI GTAC 2 cut(s) 14, 874
RseI CAYNNNNRTG 2 cut(s) 188, 755
SalI GTCGAC 1 cut(s) 218
SaqAI TTAA 1 cut(s) 338
SatI GCNGC 6 cut(s) 120, 573, 608, 611, 638, 731
Sau3AI GATC 6 cut(s) 25, 208, 543, 549, 819, 936
Sau96I GGNCC 2 cut(s) 558, 559
ScaI AGTACT 1 cut(s) 875
SchI GAGTC 4 cut(s) 225, 263, 404, 416
ScrFI CCNGG 4 cut(s) 371, 412, 557, 562
SduI GDGCHC 2 cut(s) 516, 562
SfcI CTRYAG 1 cut(s) 809
Sfr274I CTCGAG 1 cut(s) 213
SlaI CTCGAG 1 cut(s) 213
SmiMI CAYNNNNRTG 2 cut(s) 188, 755
SmlI CTYRAG 2 cut(s) 213, 529
SmoI CTYRAG 2 cut(s) 213, 529
Sse9I AATT 2 cut(s) 137, 302
StyD4I CCNGG 4 cut(s) 369, 410, 555, 560
StyI CCWWGG 1 cut(s) 108
TaaI ACNGT 2 cut(s) 466, 604
TaiI ACGT 2 cut(s) 180, 738
TaqII GACCGA 1 cut(s) 419
TasI AATT 2 cut(s) 137, 302
TatI WGTACW 1 cut(s) 873
TauI GCSGC 3 cut(s) 122, 575, 613
TfiI GAWTC 2 cut(s) 775, 864
Tru1I TTAA 1 cut(s) 338
Tru9I TTAA 1 cut(s) 338
TscAI CASTG 2 cut(s) 620, 692
TseFI GTSAC 2 cut(s) 248, 598
TseI GCWGC 3 cut(s) 607, 637, 730
Tsp45I GTSAC 2 cut(s) 248, 598
TspDTI ATGAA 2 cut(s) 767, 899
TspGWI ACGGA 3 cut(s) 43, 51, 347
TspRI CASTG 2 cut(s) 620, 692
Tth111I GACNNNGTC 1 cut(s) 406
Van91I CCANNNNNTGG 2 cut(s) 109, 192
XceI RCATGY 1 cut(s) 488
XhoI CTCGAG 1 cut(s) 213
XmiI GTMKAC 1 cut(s) 219
ZraI GACGTC 1 cut(s) 178
ZrmI AGTACT 1 cut(s) 875
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.